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Low temperature extrusion-multienzyme synergistic degradation material pretreatment method

A technology of synergistic degradation and pretreatment, applied in the fields of biofuel, food science, sugar production, etc., can solve problems such as research reports on plant fiber materials that have not yet been found, and achieve an increase in soluble substances, a reduction in the residual oil rate of the meal, and a high yield. rate increase effect

Inactive Publication Date: 2016-07-13
ZIBOHUICHUANG BIOLOGICAL SCI TECH LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] As for the pretreatment method of oil and plant fiber materials, no research report has been found on the use of low temperature extrusion-multi-enzyme synergistic degradation method to pretreat oil and plant fiber materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: A kind of low-temperature extrusion-multi-enzyme synergistic degradation dry process degerminated corn, the method for producing glucose syrup from the extrudate

[0047] This patent technology produces glucose syrup method as follows:

[0048] Firstly, the raw corn grain is degerminated by dry method to obtain dry degermed corn flour, adjusted to a moisture content of ≤70%, and at the same time adding high temperature resistant α-amylase, glucose glucoamylase and pullulanase to adjust the temperature of the extrusion sleeve ≤80°C, then, the material to be extruded is fed to the screw extrusion equipment for low-temperature extrusion processing, the extruded product is mixed with the production water for syrup production, the pH is adjusted, and high-temperature-resistant α-amylase or medium-temperature α-amylase is added. Raise the temperature to 85°C-95°C, keep it warm for 1min.-20min., cool down to 55°C-65°C, pour the above liquefied liquid into the sac...

Embodiment 2

[0049] Example 2: A method of low-temperature extrusion-multi-enzyme synergistic degradation of cell walls in soybeans, and the extrudates are used for leaching to make oil

[0050] First, soybeans are crushed to obtain soybean powder, adjusted to a moisture content of ≤70%, and cellulase, hemicellulase and pectinase are added at the same time, and the temperature of the extrusion sleeve is adjusted to ≤80°C. Then, the material to be extruded is fed The screw extrusion equipment carries out low-temperature extrusion processing. The extrudate is dried, and the moisture content is ≤10%. The dried soybean extrudate enters the extractor to extract oil, and obtain soybean meal and soybean oil. The residual oil rate of soybean meal is 0.3%. ~1.0%.

Embodiment 3

[0051] Example 3: A method for low temperature extrusion-multi-enzyme synergistic degradation of plant fibers in corn stems, leaves and roots

[0052] First, crush corn stems, leaves, and roots to obtain a crushed product, adjust it to a water content of ≤70%, add cellulase, hemicellulase, pectinase and xylanase at the same time, and adjust the temperature of the extrusion sleeve to ≤80 ℃, and then, the material to be extruded is fed to the screw extrusion equipment for low-temperature extrusion processing, and the extruded product is mixed with water for saccharification treatment to obtain glucose liquid. The soluble matter increases by 3% to 30%, and the extruded product can be used to produce feed, alcohol, sugar and its derivatives.

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Abstract

The invention belongs to the technical field of pretreatment of materials. The materials comprise starch materials, oil materials and plant fiber materials. According to the main technical characteristics, a low temperature extrusion-multienzyme synergistic degradation material pretreatment method is provided, a to-be-extruded material added with enzyme preparation and with a certain moisture content is blended before extrusion, the to-be-extruded material is fed into screw extrusion equipment to be subjected to low temperature extrusion process, temperature of an extrusion sleeve is less than or equal to 80 DEG C, material moisture content is less than or equal to 70%, multiple enzyme preparations, comprising one or more of high temperature resistant alpha-amylase, glucose saccharifying enzyme, maltase, pullulanase and protease, are added in the material before extrusion, and substances such as starch and cellulose are always degraded under the synergistic action of the added multiple enzyme preparations in a material extrusion process. Compared with the prior art, the method provided by the invention has the advantages that yield of glucose and maltose converted from starch is improved by 0.5-3%, residual oil rate of oil material meal is reduced to 0.3-1.0%, and soluble substances of the plant fiber materials are increased by 3-30%.

Description

technical field [0001] The invention belongs to the technical field of material pretreatment, and the material includes: starch materials, oil plants, and plant fiber materials. Background technique [0002] Scholars at home and abroad have shown through a large number of experimental studies that the starch degradation rate of the extruded starchy grains and their starches added with enzyme preparations is significantly faster than that of starchy grains and their starches without enzyme preparations. [0003] Extrusion enzymolysis studies by foreign scholars are all limited to extruding starchy grains and starch extrudates with only one enzyme preparation added, gelatinization, liquefaction, and saccharification processes. The representative results are as follows: P. Linko and colleagues used twin-screw extruder ClextralBC45 to extrude barley and wheat starch added with Termamylα-amylase, and the DE value was 97% after 22 hours of saccharification after extrusion. Linko e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/22C12P19/14C12P19/02C12P19/12
CPCC12P19/02C12P19/12C12P19/14C12P19/22C12P2201/00C12P21/06C12P7/06C12P7/10C12P2203/00C12P7/6445C12P7/64C13K1/00C08H99/00C08H1/00C08L91/00C08L97/02C08L89/00Y02E50/10C12N1/06C12P7/04
Inventor 申勋业申勋宇申德超
Owner ZIBOHUICHUANG BIOLOGICAL SCI TECH LTD
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