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Method for screening antidiabetic active compounds

An anti-diabetic, compound technology, applied in the field of drug screening, achieves the effects of good reproducibility, optimized conditions, and reduced preparation and separation steps

Active Publication Date: 2012-12-19
ZHEJIANG WANBANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The invention provides a method for screening antidiabetic active compounds, which overcomes the problem in the prior art that only single target or enzyme active compounds can be screened

Method used

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  • Method for screening antidiabetic active compounds
  • Method for screening antidiabetic active compounds
  • Method for screening antidiabetic active compounds

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Embodiment Construction

[0032] The present invention will be further explained below in conjunction with the accompanying drawings and embodiments, which are only used to illustrate the present invention rather than limit the present invention.

[0033] Instruments used in the examples: AgiLent 100 type liquid chromatography (AgiLent Company); Finnigan LCQ Deca XPpLus liquid chromatography-mass spectrometry instrument (Finnigan Company).

[0034]1. Active compound screening system based on multiple enzyme magnetic beads

[0035] 1. Reagent and solution preparation:

[0036] Maltase, sucrase, porcine pancreatic lipase (Simga); carboxyl magnetic beads Dynabeads® MyOne CarboxyLic Acid, specification: 10mg / mL (invitrogen); 1-ethyl-3-(3-dimethylaminopropyl) Carbodiimide hydrochloride (EDC, sigma company); 2-(N-morpholine) ethanesulfonic acid (MES, sigma company); N-hydroxysuccinimide (NHS, sigma company).

[0037] Preparation of 25mmol / L MES aqueous solution: Weigh an appropriate amount of MES, add wate...

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Abstract

The invention discloses a method for screening antidiabetic active compounds. The method is characterized by comprising the steps of: (1) preparing bonding enzyme magnetic beads; (2) using at least two kinds of the bonding enzyme magnetic beads to conduct preferential adsorption on a traditional Chinese medicine solution or a natural extractive solution, enabling target proteins bonded on different bonding enzyme magnetic beads to be mutually different, and enabling the target proteins to be maltase, sucrase or lipase; (3) separating compounds jointly absorbed by all bonding enzyme magnetic beads to serve as a sample set; and (4) screening the antidiabetic active compounds from the sample set through inhibitory activity tests directing at the target proteins. The method for screening antidiabetic active compounds can screen the antidiabetic active compounds in a traditional Chinese medicine or a natural extractive aiming at multiple target points or enzymes, is applicable to development of anti-diabetic medicines, simultaneously reduces compound preparation and separation steps during initial screening in a traditional method, improves screening efficiency and shortens screening times.

Description

technical field [0001] The invention relates to the field of drug screening, in particular to a method for screening antidiabetic active compounds. Background technique [0002] Diabetes is a chronic metabolic disorder. If diabetes is not well controlled, it will lead to various complications, which can damage multiple tissues and organs in the human body, resulting in disability and high mortality. Diabetes has become a global epidemic. According to the report of WHO, there are about 160 million diabetic patients in the world. The total number of diabetics in my country has reached more than 40 million, second only to India, ranking second in the world, and the harm is huge. [0003] At present, the main intervention strategies for the treatment of diabetes are diet control, reducing the content of ingested carbohydrates, etc. Type I diabetes can only rely on insulin-dependent treatment because the cells of the pancreas that produce insulin have been completely damaged. T...

Claims

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Application Information

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IPC IPC(8): G01N33/15
Inventor 程翼宇王毅
Owner ZHEJIANG WANBANG PHARMA
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