277 results about "Ethanesulfonic acid" patented technology

The invention relates to a ring structure betaine and double-tailed hydrophobic association polymer fracturing thickening agent and a preparation method thereof. The thickening agent is formed by copolymerization of four monomers, namely acrylamide, 2-allyl methylamino sodium ethanesulfonate, methyl diallyl sulfoethyl betaine and hydrophobic monomer double-tailed long-chain alkyl methyl allyl ammonium chloride. The preparation method comprises the following steps: firstly adding distilled water, then sequentially adding acrylamide, 2-allyl methylamino sodium ethanesulfonate, methyl diallyl sulfoethyl betaine and a hydrophobic monomer double-tailed long-chain alkyl methyl allyl ammonium chloride, adjusting the total concentration, introducing N2 to remove oxygen, adding an initiator, and reacting to prepare an AM / AMES / MDASBA / DCnMAAC quadripolymer, namely the polymer fracturing thickening agent. The thickening agent provided by the invention has good water solubility, and shows good salt resistance in mixed brine with the mineralization degree of greater than or equal to 30000 mg / L. The prepared linear solution and gel formed after crosslinking have high viscosity retention capacity at the temperature of 150 DEG C and the shearing rate of 170 s <-1 >, and the actual construction requirement for fracturing yield increase with the on-site mineralization degree larger than or equal to 30000 mg / L is met.

The invention relates to a preparation method of a high-hardness and high-sulfate-radical water quality scale inhibitor, and belongs to the technical field of water treatment.The preparation method comprises the steps that sodium carboxymethyl cellulose-sulfur quantum dots are prepared by using carboxymethyl cellulose as a stabilizer and then subjected to a copolymerization reaction with 2-acrylamido-2-methylpropanesulfonic acid and itaconic acid, and the high-hardness and high-sulfate-radical water quality scale inhibitor is obtained. The preparation method comprises the following steps: carrying out a reaction on sodium carboxymethyl cellulose and sulfur quantum dots to obtain modified sodium carboxymethyl cellulose-sulfur quantum dots, and carrying out a reaction on polysuccinimide, 4-amino phthalic acid and 2-aminoethanesulfonic acid to obtain a polyaspartic acid derivative; the modified sodium carboxymethyl cellulose-sulfur quantum dots and the polyaspartic acid derivative are mixed to serve as the scale inhibitor, the scale inhibition rate of the scale inhibitor is remarkably increased through the synergistic effect of the modified sodium carboxymethyl cellulose-sulfur quantum dots and the polyaspartic acid derivative, and meanwhile, due to the fact that a large number of new hydrogen bonds are generated between the modified sodium carboxymethyl cellulose-sulfur quantum dots and the polyaspartic acid derivative, the scale inhibition rate of the scale inhibitor is increased. The stability of a polymer structure is improved, so that the temperature resistance of the scale inhibitor is improved. The scale inhibitor is green and environment-friendly, and has excellent scale inhibition efficiency on high-hardness and high-sulfate-radical water.

The invention relates to a betaine type double-tailed hydrophobic association polymer fracturing thickening agent and a preparation method thereof. The thickening agent is prepared by the following steps: copolymerizing four monomers, namely acrylamide, 2-allyl methylamino sodium ethanesulfonate, methyl diallyl hydroxypropyl sulfobetaine and double-tailed long-chain alkyl allyl sulfoethyl betaine to form gel, chopping, hydrolyzing, neutralizing and purifying. The preparation method comprises the following steps: firstly adding distilled water, adding acrylamide, 2-allylmethylamino sodium ethanesulfonate, methyldiallyl hydroxypropyl sulfobetaine and other remaining raw materials, adjusting the total concentration, introducing N2 to remove oxygen, adding an initiator, reacting to prepare gel, chopping the gel, and hydrolyzing to obtain the water-soluble polymer. The fracturing thickening agent has good water solubility and shows good salt resistance in mixed saline water with the mineralization degree larger than or equal to 30000 mg / L, a prepared linear solution and jelly formed after crosslinking have high viscosity retention capacity at the temperature of 150 DEG C and the shearing rate of 170 s <-1 >, and the actual construction requirement for fracturing yield increase with the on-site mineralization degree larger than or equal to 30000 mg / L is met.

The invention discloses a high-sensitivity cardiac troponin-T electrochemiluminescence detection kit. The kit comprises a magnetic microsphere reagent coated by a high-sensitivity cardiac troponin-T capture antibody and a high-sensitivity cardiac troponin-T detection antibody reagent labeled by an electrically neutral ruthenium compound, the structural general formula of the electrically neutral ruthenium compound is # imgabs0 # n = 0-9; the magnetic microsphere reagent coated with the high-sensitivity cardiac troponin-T capture antibody comprises a magnetic microsphere coated with the high-sensitivity cardiac troponin-T capture antibody and a diluent; the electrically neutral ruthenium compound labeled high-sensitivity cardiac troponin-T detection antibody reagent comprises an electrically neutral ruthenium compound labeled high-sensitivity cardiac troponin-T detection antibody and a diluent; the diluent is prepared from piperazine-1, 4-diethyl sulfonic acid or 3-(N-morpholinyl)-2-hydroxy propanesulfonic acid. When the high-sensitivity cardiac troponin-T electrochemiluminescence detection kit is used for detection, a reaction system does not contain streptavidin coated magnetic beads or biotinylated antibodies, so that interference of biotin in a sample is avoided.

The invention discloses a liquefying agent, application thereof and a nucleic acid detection system, the liquefying agent comprises strong base, acetylcysteine and 2-(N-morpholine) ethanesulfonic acid; in the liquefying agent, the concentration of the strong base is 0.2 mol / L to 1 mol / L; the concentration of the acetylcysteine is 3 to 300 mmol / L; the concentration of the 2-(N-morpholine) ethanesulfonic acid ranges from 2 mmol / L to 5 mmol / L. Through the synergistic effect of the strong base, the acetylcysteine and the 2-(N-morpholine) ethanesulfonic acid, the liquefaction time can be remarkably shortened, the viscous biological sample treatment efficiency and speed can be improved, and the viscous biological sample treated by the liquefier can be directly used for nucleic acid amplification; and the viscous biological sample treated by the liquefying agent can be stored for a long time, the accuracy and reliability of nucleic acid detection are ensured by long-time stability, and the credibility of a detection result is improved.

The invention relates to a double-tailed hydrophobic associated polymer oil-displacing agent with a benzene ring structure and a preparation method thereof. The oil-displacing agent is formed by copolymerizing three monomers, namely acrylamide, 2-allyl methylamino sodium ethanesulfonate and a hydrophobic monomer double-tailed long-chain alkyl methyl (p-3-carboxyl acrylamide benzyl) ammonium chloride with the benzene ring structure. The preparation method comprises the following steps: firstly adding distilled water, then adding acrylamide, 2-allyl methylamino sodium ethanesulfonate and a hydrophobic monomer double-tailed long-chain alkyl methyl (p-3-carboxyl acrylamide benzyl) ammonium chloride with a benzene ring structure, supplementing water, adjusting the total concentration, introducing N2 to remove oxygen, adding an initiator, and reacting at a certain temperature to prepare the terpolymer. The prepared polymer oil-displacing agent has good water solubility, temperature resistance and shearing resistance, shows good salt resistance in saline water with the mineralization degree larger than or equal to 30000 mg / L and shows a good oil-displacing effect in a heterogeneous core oil-displacing experiment, and the crude oil recovery rate can be increased by 10% or above.

The invention relates to the technical field of oilfield chemistry, in particular to an aminoethanesulfonic acid type surfactant and a preparation method and application thereof.The preparation method of the aminoethanesulfonic acid type surfactant comprises the steps that firstly, fatty amine is dissolved in a first solvent, a sodium chloroethanesulfonate aqueous solution is added, and N-long-chain alkyl-2-sodium aminoethanesulfonate is obtained through a reaction; and 2, dissolving a required amount of N-long-chain alkyl-2-sodium aminoethanesulfonate in a second solvent, adding a required amount of methyl acrylate, and reacting to obtain the aminoethanesulfonic acid type surfactant. The aminoethanesulfonic acid type surfactant disclosed by the invention contains long-chain alkyl, amino, ester groups and sulfonic acid groups, so that the aminoethanesulfonic acid type surfactant has good surface activity and can meet the development requirements of oil and gas fields. The microemulsion cleanup additive prepared from the aminoethanesulfonic acid type surfactant can effectively reduce the surface tension and the interfacial tension of an aqueous solution, and the preparation method is easy and convenient to operate, mild in condition and suitable for industrial production.

The invention belongs to the technical field of cosmetics, and discloses a superconductive centella asiatica extract as well as a preparation method and application thereof. The superconductive centella asiatica extract has a core-shell structure, an inner core of the superconductive centella asiatica extract comprises centella asiatica extract, a shell of the superconductive centella asiatica extract comprises ionic liquid, and the ionic liquid comprises ethyoxyl diglycol, urea, betaine, N-ethyl pyrrolidone and hydroxyethyl piperazine ethanesulfonic acid. According to the invention, ethyoxyl diglycol, urea, betaine, N-ethyl pyrrolidone and hydroxyethyl piperazine ethanesulfonic acid are compounded to obtain the ionic liquid, and the centella asiatica extract is wrapped in the ionic liquid, so that the stability, transdermal absorption rate and bioavailability of the centella asiatica extract can be effectively improved.

The invention relates to a bisulfonate hydrophobic association polymer oil-displacing agent with a benzene ring structure and a preparation method thereof. The polymer oil-displacing agent is formed by copolymerization of three monomers, namely acrylamide, sodium 2-allyl methylamino ethanesulfonate and long-chain alkyl disulfoethyl (4-vinyl benzyl) betaine which is a hydrophobic monomer with the benzene ring structure. The preparation method comprises the following steps: firstly adding distilled water, sequentially adding acrylamide, 2-allylmethylamino sodium ethanesulfonate and long-chain alkyl disulfoethyl (4-vinyl benzyl) betaine, adding water to adjust the total concentration, introducing N2 to remove oxygen, adding an initiator, and reacting at a certain temperature to obtain the terpolymer. The polymer oil-displacing agent prepared by the invention has good water solubility and good temperature resistance and shear resistance, shows good salt resistance in saline water with the degree of mineralization being greater than or equal to 30000 mg / L, shows a good oil-displacing effect in heterogeneous rock cores, and can improve the crude oil recovery rate by 10% or above.

A method for preparing exosomes is provided. The method includes preparing a human platelet, treating the human platelet with a buffer to obtain a platelet solution, centrifuging the platelet solution, and collecting supernatant of the centrifuged platelet solution to obtain an exosome solution. The buffer is calcium ion buffer, phosphate buffered saline (PBS), tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) buffer, or 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) buffer.

The invention provides a triazine covalent organic framework film as well as a preparation method and application thereof. A preparation method of a triazine covalent organic framework membrane comprises the following steps: (1) soaking and washing a polyacrylonitrile ultrafiltration membrane in a strong alkali solution to obtain a pretreated ultrafiltration membrane; (2) soaking the pretreated ultrafiltration membrane in a 2-morpholine ethanesulfonic acid buffer solution containing N-hydroxysuccinimide and 1-ethyl-(3-dimethylaminopropyl) carbodiimide, and washing with water to obtain an activated ultrafiltration membrane; (3) soaking the activated ultrafiltration membrane in a first organic phase solution of a polyamine monomer and 1, 4-dioxane, and washing with water; soaking in a second organic phase solution of a polyaldehyde monomer and 1, 4-dioxane to obtain a modified ultrafiltration membrane; and (4) immersing the modified ultrafiltration membrane into a mixed solution composed of a polyamine monomer, a polyaldehyde monomer, acetic acid and 1, 4-dioxane, and cleaning to obtain the triazine covalent organic framework membrane. The triazine covalent organic framework membrane provided by the invention has good separation performance and catalytic oxidation performance.

The present invention concerns pharmaceutically acceptable salts of (S)-1-(1-(3-chlorophenyl)- 2-(dimethylamino)ethyl)-4-(5-morpholino-1H-pyrrolo[2,3-b]pyridin-3-yl)pyridin-2(1 H)-one of the formula (I) wherein said salt is selected from a hydrochloride salt, a maleate salt, an esylate salt, an oxoglutarate salt, a malonate salt and an oxalate salt.

The invention discloses a whitening and freckle-removing essence which comprises the following components: a calendula extract, pure water, 1, 2-pentanediol, methylpropanediol, carbomer, arginine, nicotinamide, a freckle-removing essence, a natural plant penetration enhancer, hydroxyethyl piperazine ethanesulfonic acid, small-molecule ellagic acid, dipropylene glycol, 1, 2-propylene glycol, ethanol and water. The composition is prepared from 1, 2-hexanediol, caprylyl glycol, ethylhexylglycerin, raspberry ketone glucoside and a PEG / PPG-17 / 6 copolymer. The invention further discloses a production process of the whitening and freckle-removing essence water. The invention has the following advantages: with pure natural plants as a main product, the skin has obvious change after being used for a week, specifically, pregnancy spots, sunburn, chloasma, pigmentation after inflammation and other color spots on the face can be reduced and faded, the face skin can be whitened, the face is mild and non-irritating, and the effects of improving uneven skin color, brightening skin color and the like can be achieved.

To provide an electrolytic silver germanium alloy plating solution capable of codepositing a sufficient amount of germanium in a film, having good liquid stability, and hardly causing foaming.SOLUTION: The problem is solved by an electrolytic silver germanium alloy plating solution containing a silver source and a germanium source characterized by containing a nitrogen-containing coordination compound having a molecular weight of 500 or less. Specific examples of the nitrogen-containing coordination compound include N,N-di(2-hydroxyethyl)glycine, diethanolamine, N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, triethylenetetramine, glycine, tris(hydroxymethyl) aminomethane, N-methylglucamine, bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane, tricin, N,N,N',N'-tetrakis(2-hydroxypropyl)ethylenediamine, and salts of these compounds.SELECTED DRAWING: None

The invention provides a triglyceride determination kit, the kit comprises a reagent 1 and a reagent 2, the reagent 1 comprises the following main components: piperazine-1, 4-diethyl sulfonic acid (PIPES) buffer solution (pH 6.8), glycerophosphate oxidase (GPO), peroxidase (POD), triadenosine phosphate (ATP), chromogen, a surfactant, a stabilizer and a preservative; and the reagent 2 comprises the following main components: a piperazine-1, 4-diethyl sulfonic acid (PIPES) buffer solution (pH is 6.5), lipoprotein esterase (LPL), glycerol kinase (GK), 4-aminoantipyrine (4-AA), a surfactant, a stabilizer and a preservative. By optimizing a chromogen substrate in the reagent and adding potassium ferrocyanide, the linear range of the prepared kit is wider, and the bottle opening test is more stable.

The invention discloses a preserving fluid for stably preserving sample DNA at normal temperature and a preparation method thereof, and belongs to the technical field of biological sample preservation. The preserving fluid comprises a lysis system, a nucleic acid protection system and a buffering and stabilizing system, the cracking system comprises a composite surfactant and an enzymolysis auxiliary agent; the composite surface active agent is prepared from polyether polyol fatty acid ester and cocamidopropyl hydroxy sulfobetaine; the enzymolysis auxiliary agent comprises lysozyme Lyso-V and protease K; the nucleic acid protection system comprises a nitrogen heterocyclic polyamine-carboxylic acid derivative and dextran sulfate; the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N' ', N ''tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N''-triacetic acid, disodium ethylene diamine tetraacetate-nitrogen heterocyclic derivative, and diethylenetriamine pentaacetic acid-piperazine derivative, and the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N '', N'' tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N ''-triacetic acid. The buffering and stabilizing system comprises an amphoteric buffering agent and a polymer stabilizer; the amphoteric buffering agent comprises 2-(N-morpholino) ethanesulfonic acid and N-tri (hydroxymethyl) methylglycine.

The invention relates to a high-solubility sulfonation-improved aqueous iron-based flow battery electrolyte, belongs to the technical field of flow battery energy storage, and aims to solve the problem of low solubility of the iron-based flow battery electrolyte, the novel high-solubility iron-based flow battery electrolyte is synthesized by adopting an asymmetric sulfonation ligand to chelate an iron element. And the concentration of the active substance at normal temperature can reach 1.3 M or above. The electrolyte is mainly composed of one or more iron-containing salts, and the iron-containing salts are coordinated with 3-trihydroxymethyl methylamino-2-hydroxy propanesulfonic acid (TAPSO), trihydroxymethyl methylamino propanesulfonic acid (TAPS), trihydroxymethyl methylamino ethanesulfonic acid (TES) or derivative chemicals thereof to form an aqueous solution of a coordination compound. The electrolyte is matched with ferrocyanide, can provide voltage of 1.3 V or above, shows excellent cycling stability while keeping high solubility, and creates new possibility for development and application of a flow battery technology.

The invention belongs to the field of oilfield chemistry, and relates to self-thickening polyacrylamide as well as a preparation method and application thereof. The preparation method of the self-thickening polyacrylamide comprises the following steps: dissolving acrylamide, 2-acrylamide-2-phenyl ethanesulfonic acid, hydroxyl-N-allylaniline, N-hydroxymethyl acrylamide and a cosolvent in deionized water, and adjusting the pH value to obtain a homopolymerization solution; cooling the homopolymerization solution, introducing nitrogen, adding an initiator, introducing nitrogen again, carrying out a polymerization reaction, and after the reaction is completed, carrying out heating and heat preservation, and carrying out an aging reaction, so as to obtain a polyacrylamide rubber block; and cutting, granulating, drying, crushing and screening the polyacrylamide gel block to obtain the self-thickening polyacrylamide. The self-thickening polyacrylamide disclosed by the invention contains hydroxyl-N-allylaniline and N-hydroxymethyl acrylamide monomers, gel is generated along with time extension under the oil reservoir condition, the profile control effect is improved, and the problems of phase separation and chromatographic effect caused by migration velocity difference of a conventional gel system are avoided.

The invention discloses an artificial blood vessel and a preparation method thereof, and the preparation method comprises the following steps: uniformly mixing an erythrocyte ghost suspension and melanin nanoparticles to obtain a mixed solution; carrying out ultrasonic treatment and extrusion on the mixed solution to obtain a melanin nano-vesicle solution; carrying out electrostatic spinning on the polycaprolactone solution to obtain a polycaprolactone electrostatic spinning blood vessel; soaking the polycaprolactone electrostatic spinning blood vessel in an ethylenediamine solution, and then washing the polycaprolactone electrostatic spinning blood vessel with a phosphate buffer solution; then soaking in a 4-morpholine ethanesulfonic acid solution; finally, carrying out heparinization in a 4-morpholine ethanesulfonic acid solution containing 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and heparin; and incubating the heparin-modified polycaprolactone electrostatic spinning blood vessel in the melanin nano-vesicle solution to obtain the artificial blood vessel. The melanin nano-vesicles are modified, so that the anticoagulation performance of erythrocyte membranes is reserved, and meanwhile, the scavenging capacity of melanin nano-particles on reactive oxygen free radicals is reserved.

The invention discloses an anti-pollution ultrafiltration membrane and a preparation method thereof, and relates to the technical field of organic polymer materials. The anti-pollution ultrafiltration membrane is prepared from the following raw materials by weight: 80 parts of hydrophilic modified polyethersulfone, 2-4 parts of coupling agent, 3-5 parts of Al-Nb-Sc-O-Te, 15-25 parts of additive, 5-8 parts of polyethylene glycol diacrylate, 2-4 parts of 3-[N,N-dimethyl-[2-(2-methylprop-2-enoyloxy)ethyl]ammonium]propane-1-sulfonic acid inner salt, and 3-5 parts of 1-allyl-3-vinyl imidazole chloride; the hydrophilic modified polyethersulfone is trishydroxymethylaminomethanesulfonic acid modified polyethersulfone. The anti-pollution ultrafiltration membrane has good anti-pollution performance, sufficient pure water flux, and high BSA retention rate.

The invention relates to a composition with whitening and skin tendering effects, essence lotion and a preparation method and application of the essence lotion, in the composition with the whitening and skin tendering effects, the weight ratio of achillea millefolium extract to hydroxyethyl piperazine ethanesulfonic acid to ormosia henryi bark extract is (0.1-1): (0.5-8): (0.05-0.5). The essence lotion is prepared from the following components in percentage by weight: 0.75 to 6 percent of composition with skin whitening and tendering effects, 3 to 8 percent of emollient, 0.5 to 2 percent of emulsifier, 0.01 to 1 percent of filler, 0.5 to 2 percent of thickener, 0.8 to 1.5 percent of preservative, 0.02 to 0.2 percent of daily essence, 0.1 to 10 percent of skin conditioner, 0.1 to 10 percent of humectant and the balance of deionized water. The main components have a synergistic effect, can effectively regulate and control the information network effect of endothelin and reduce the differentiation, proliferation and the like of melanocytes in the first step of melanin generation by inhibiting the activity of tyrosinase, can promote cell autophagy and effectively metabolize melanin and lipofuscin generated in the skin, and achieves the effects of whitening, whitening, whitening and the like. The skin tendering effect is realized.

The invention belongs to the technical field of development of nintedanib preparations, and particularly relates to a nintedanib suspension preparation, a preparation method thereof and application of the nintedanib suspension preparation in treatment of pulmonary fibrosis. The preparation method comprises the following steps: dissolving a phospholipid material in an organic solvent at 50-70 DEG C, and preserving heat to obtain a solution A; dissolving inorganic salt in water for injection, and preserving heat to obtain a solution B; dissolving the ethanesulfonic acid nintedanib in the water for injection to obtain a solution C; dissolving an osmotic pressure regulator in the water for injection to obtain a solution D; mixing the solution A and the solution B by using microfluidics to obtain a suspension E; filtering through a tangential flow membrane to remove the organic solvent in the suspension E, adding the solution C, keeping the temperature for a period of time, adding the solution D as required, and uniformly mixing; and filtering the suspension with a sterilization filter to obtain the nintedanib suspension preparation. The liposome suspension prepared from the nintedanib prepared by the invention is stable in physical state and excellent in slow release effect, is suitable for low-dose inhalation administration in the lung, and is beneficial to improving the treatment effect and reducing side effects.

The invention relates to a bisulfonate hydrophobic associated polymer oil-displacing agent with a cyclic structure and a preparation method thereof. The oil-displacing agent is formed by copolymerization of four monomers, namely acrylamide, sodium 2-allyl methylamino ethanesulfonate, diallyl disulfoethyl betaine and long-chain alkyl dimethyl (p-3-carboxyl acrylamide benzyl) ammonium chloride which is a hydrophobic monomer with a benzene ring structure. The preparation method comprises the following steps: firstly, adding distilled water, then sequentially adding acrylamide, 2-allyl methylamino sodium ethanesulfonate, diallyl disulfoethyl betaine and a hydrophobic monomer long-chain alkyl dimethyl (p-3-carboxyl acrylamide benzyl) ammonium chloride with a benzene ring structure, supplementing water, adjusting the total concentration, introducing N2 to remove oxygen, adding an initiator, and stirring to obtain the water-absorbent polymer. And reacting at a certain temperature to obtain the quadripolymer. The prepared oil-displacing agent has good water solubility, temperature resistance and shearing resistance, shows good salt resistance in saline water with the mineralization degree larger than or equal to 30000 mg / L, shows a good oil-displacing effect in heterogeneous rock cores, and can improve the crude oil recovery rate by 10% or above.

The invention provides synthesis of a T-2 toxin antigen and preparation and application of a monoclonal antibody. Relates to the technical field of biotoxin detection. The preparation method of the T-2 toxin antigen comprises the following steps: step 1, synthesizing an immunogen T-2-BSA by adopting a carbodiimide method, dissolving 2mg of T-2 adipic acid with 0.5 mL of dimethylformamide, and recording as a solution A; 5 mg of bovine serum albumin and 3 mg of EDC are dissolved with a 2-morpholine ethanesulfonic acid buffer solution with the pH being 5.5, and the solution is marked as a solution B; step 2, under the condition of stirring, dropwise adding the solution A into the solution B, stirring for 18 hours in a dark place at 4 DEG C, then dialyzing for 3 days by using a phosphate buffer solution, changing the solution once a day, centrifuging the dialysate for 10 minutes at 3,500 r / min, and discarding the precipitate; the adipic acid is coupled with the T-2 toxin through a chemical method, and the unique step enriches the structural characteristics of the hapten, so that the prepared T-2 toxin complete antigen is excellent in immune effect, can efficiently excite immune response of an organism, and shows high specificity and sensitivity.

The invention relates to the field of external cosmetics for eyes, in particular to double-resistance eye cream and a preparation method thereof. The double-resistance eye cream is prepared from the following components in percentage by mass: 4-hydroxyethyl piperazine ethanesulfonic acid, betaine, dipropylene glycol, allantoin, a chelating agent and water, an ammonium acryloyldimethyl taurate / VP copolymer; the invention relates to a skin care product which is prepared from the following raw materials: cetostearyl alcohol wheat straw glycoside, cetostearyl alcohol, glyceryl stearate, PEG-100 stearate, caprylic / capric triglyceride, emblic leafflower fruit extract, polydimethylsiloxane, tocopheryl acetate and behenyl alcohol. Tranexamic acid; a whitening supplement; a bacteriostatic agent; and pine mushroom extract. Aiming at three causes causing periorbital problems, such as skin oxidation, skin saccharification and inflammatory reaction, through a multi-section target spot effect, multiple chains snip dark eyes, refine and lighten wrinkles, and restore elasticity, smoothness and brightness.

The invention belongs to the technical field of polyurethane resin, and particularly relates to a preparation method and application of thermoplastic carbon fiber sizing agent waterborne polyurethane resin.The preparation method comprises the following steps that (1) a prepolymer is prepared, specifically, 72-76 parts of polyester polyol and 20-24 parts of isocyanate are mixed and react, 13-16 parts of dimethylolpropionic acid and 6-9 parts of 1, 4-dihydroxy-2, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3, 4-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 3-trimethyl-1, 2, 2-dimethyl piperazine and 40-50 parts of acetone react, 1-3 parts of triethylamine is added, a reaction is conducted for 5-10 min, then 2-4 parts of sodium ethylenediamine ethanesulfonate is added, and a prepolymer is obtained; (2) emulsifying: transferring the prepolymer into an emulsifier, adding 280-300 parts of an ice-water mixture, stirring, adding 6-8 parts of an ethylenediamine aqueous solution with the mass fraction of 18-20%, standing for 20-24 hours, removing acetone, adding a coalescing agent, a wetting agent, a defoaming agent and a lubricating agent, and uniformly stirring to obtain the waterborne polyurethane resin.

The invention belongs to the technical field of organoid culture, and particularly relates to tissue digestive juice for organoid culture as well as a preparation method and application of the tissue digestive juice. According to the tissue digestive juice, a DMEM / F-12 culture medium which contains L-glutamine and does not contain 4-hydroxyethyl piperazine ethanesulfonic acid is used as a basic culture medium, calcium chloride, collagenase I type, collagenase III type, V type hyaluronidase, deoxyribonuclease I, 4-hydroxyethyl piperazine ethanesulfonic acid and Y-27632 are combined and have a synergistic effect, cell damage is reduced, meanwhile, the tissue digestive juice has the advantages that the tissue digestive juice can be used for improving the tissue digestive juice quality, and the tissue digestive juice has a good application prospect. The digestion time is shortened, the tissue digestion efficiency is improved, and the culture success rate of the organoid is improved.

The invention relates to the technical field of zearalenone detection, in particular to zearalenone immunomagnetic beads as well as a preparation method and application thereof. The method specifically comprises the following steps: (1) cleaning N-hydroxysuccinimide activated agarose magnetic beads with ethanol, removing ethanol, adding a 2-morpholinoethanesulfonic acid buffer solution and a ZEN monoclonal antibody for mixed incubation, and discarding an upper solution; and (2) adding a glycine-containing buffer solution into the incubated magnetic beads, mixing, sealing, carrying out magnetic separation, and leaching to obtain the ZEN immunomagnetic beads. According to the method, IMBs high-flux full-automatic purification is combined with a high performance liquid chromatograph, the method which is rapid and convenient and can automatically treat ZEN in feed and feed raw material samples in batches is established, and the first feed industry association group standard about the IMBs purification technology is researched and formulated on the basis of the method; and a method reference is provided for prevention and control of fungaltoxin pollution in basic detection laboratories and feed enterprises.

The invention relates to responsive hydrogel loaded with bionic nano-vesicles as well as a preparation method and application of the responsive hydrogel, and belongs to the technical field of medical hydrogel. The method comprises the steps that odontogenic mesenchymal stem cells are stimulated by a chemical vesicle stimulant and then mixed with melatonin mother liquor for extrusion, the bionic nano vesicles are obtained, the chemical vesicle stimulant contains a buffer solution, a dithiothreitol solution and a paraformaldehyde solution, and the buffer solution contains 8.5-9 g / L of sodium chloride, 0.2-0.24 g / L of calcium dichloride and 2.3-2.4 g / L of 4-hydroxyethyl piperazine ethanesulfonic acid; 6-arm-PEG-pp-NH2 coupled with polypeptide capable of being cut by matrix metalloproteinase, six-arm polyethylene glycol succinimide glutarate and the bionic nano-vesicle are prepared into a first precursor solution and a second precursor solution respectively, the first precursor solution and the second precursor solution are mixed and subjected to standing to form gel, and the responsive hydrogel is prepared. According to the invention, the responsive hydrogel capable of obviously enhancing bone regeneration capacity is obtained.

The invention discloses a carbon dioxide adsorbent based on a silver metal organic framework and a production method thereof.The carbon dioxide adsorbent is obtained by compounding aminoethanesulfonic acid and the silver metal organic framework according to the mass ratio of 1: 1, and the production method comprises the steps that S1, a bimetal framework is synthesized; s2, preparing a silver metal organic framework; s3, amino ethanesulfonic acid modification; by compounding the aminoethanesulfonic acid and the silver metal organic framework, the distribution of metal silver in the organic framework can be optimized, and CO2 chemical adsorption is enhanced through double metal nodes and easy acidic sites; metal silver forms a silver metal framework through coordination, aminoethanesulfonic acid modifies the surface of a pore channel through a sulfonic acid group, physical adsorption and chemical adsorption on CO2 are enhanced, the obtained carbon dioxide adsorbent can be combined with the surface plasma resonance effect, light energy can be efficiently converted into heat energy under visible light, and therefore desorption energy consumption is reduced.