132 results about "Ethanesulfonic acid" patented technology

The invention relates to the technical field of oilfield chemistry, in particular to an aminoethanesulfonic acid type surfactant and a preparation method and application thereof.The preparation method of the aminoethanesulfonic acid type surfactant comprises the steps that firstly, fatty amine is dissolved in a first solvent, a sodium chloroethanesulfonate aqueous solution is added, and N-long-chain alkyl-2-sodium aminoethanesulfonate is obtained through a reaction; and 2, dissolving a required amount of N-long-chain alkyl-2-sodium aminoethanesulfonate in a second solvent, adding a required amount of methyl acrylate, and reacting to obtain the aminoethanesulfonic acid type surfactant. The aminoethanesulfonic acid type surfactant disclosed by the invention contains long-chain alkyl, amino, ester groups and sulfonic acid groups, so that the aminoethanesulfonic acid type surfactant has good surface activity and can meet the development requirements of oil and gas fields. The microemulsion cleanup additive prepared from the aminoethanesulfonic acid type surfactant can effectively reduce the surface tension and the interfacial tension of an aqueous solution, and the preparation method is easy and convenient to operate, mild in condition and suitable for industrial production.

A method for preparing exosomes is provided. The method includes preparing a human platelet, treating the human platelet with a buffer to obtain a platelet solution, centrifuging the platelet solution, and collecting supernatant of the centrifuged platelet solution to obtain an exosome solution. The buffer is calcium ion buffer, phosphate buffered saline (PBS), tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl) buffer, or 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) buffer.

The invention discloses a preserving fluid for stably preserving sample DNA at normal temperature and a preparation method thereof, and belongs to the technical field of biological sample preservation. The preserving fluid comprises a lysis system, a nucleic acid protection system and a buffering and stabilizing system, the cracking system comprises a composite surfactant and an enzymolysis auxiliary agent; the composite surface active agent is prepared from polyether polyol fatty acid ester and cocamidopropyl hydroxy sulfobetaine; the enzymolysis auxiliary agent comprises lysozyme Lyso-V and protease K; the nucleic acid protection system comprises a nitrogen heterocyclic polyamine-carboxylic acid derivative and dextran sulfate; the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N' ', N ''tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N''-triacetic acid, disodium ethylene diamine tetraacetate-nitrogen heterocyclic derivative, and diethylenetriamine pentaacetic acid-piperazine derivative, and the nitrogen heterocyclic polyamine-carboxylic acid derivative comprises 1, 4, 7, 10-tetraazacyclododecane-N, N ', N '', N'' tetraacetic acid, 1, 4, 7-triazacyclononane-N, N', N ''-triacetic acid. The buffering and stabilizing system comprises an amphoteric buffering agent and a polymer stabilizer; the amphoteric buffering agent comprises 2-(N-morpholino) ethanesulfonic acid and N-tri (hydroxymethyl) methylglycine.

The invention provides synthesis of a T-2 toxin antigen and preparation and application of a monoclonal antibody. Relates to the technical field of biotoxin detection. The preparation method of the T-2 toxin antigen comprises the following steps: step 1, synthesizing an immunogen T-2-BSA by adopting a carbodiimide method, dissolving 2mg of T-2 adipic acid with 0.5 mL of dimethylformamide, and recording as a solution A; 5 mg of bovine serum albumin and 3 mg of EDC are dissolved with a 2-morpholine ethanesulfonic acid buffer solution with the pH being 5.5, and the solution is marked as a solution B; step 2, under the condition of stirring, dropwise adding the solution A into the solution B, stirring for 18 hours in a dark place at 4 DEG C, then dialyzing for 3 days by using a phosphate buffer solution, changing the solution once a day, centrifuging the dialysate for 10 minutes at 3,500 r / min, and discarding the precipitate; the adipic acid is coupled with the T-2 toxin through a chemical method, and the unique step enriches the structural characteristics of the hapten, so that the prepared T-2 toxin complete antigen is excellent in immune effect, can efficiently excite immune response of an organism, and shows high specificity and sensitivity.

The invention discloses a carbon dioxide adsorbent based on a silver metal organic framework and a production method thereof.The carbon dioxide adsorbent is obtained by compounding aminoethanesulfonic acid and the silver metal organic framework according to the mass ratio of 1: 1, and the production method comprises the steps that S1, a bimetal framework is synthesized; s2, preparing a silver metal organic framework; s3, amino ethanesulfonic acid modification; by compounding the aminoethanesulfonic acid and the silver metal organic framework, the distribution of metal silver in the organic framework can be optimized, and CO2 chemical adsorption is enhanced through double metal nodes and easy acidic sites; metal silver forms a silver metal framework through coordination, aminoethanesulfonic acid modifies the surface of a pore channel through a sulfonic acid group, physical adsorption and chemical adsorption on CO2 are enhanced, the obtained carbon dioxide adsorbent can be combined with the surface plasma resonance effect, light energy can be efficiently converted into heat energy under visible light, and therefore desorption energy consumption is reduced.

The invention relates to the technical field of ink, and discloses environment-friendly water-based gravure ink for automotive upholstery and a preparation method thereof.The preparation method comprises the steps that 2-[3-(2-(methacryloyloxy) ethyl) ureido] ethanesulfonic acid and chitosan are subjected to a graft polymerization reaction to obtain modified chitosan, the modified chitosan, pigment, a dispersing agent and the like are added into water-based acrylic resin, and the environment-friendly water-based gravure ink is prepared. The environment-friendly water-based gravure ink for the automobile interior trim parts is obtained. The modified chitosan contains a large amount of hydrophilic sulfonate groups, has excellent dispersibility in the water-based acrylic resin, is grafted with polyacrylate molecular chains, has better compatibility with the acrylic resin, and is beneficial to improving the tensile strength and mechanical properties of the ink adhesive film. The modified chitosan contains a large amount of ureido groups and can form hydrogen bond interaction force with polyurethane, polycarbonate and the like, so that the cohesiveness and adhesive force between the ink and plastic matrixes such as polyurethane, polycarbonate and the like are improved.

The invention relates to the technical field of all-vanadium redox flow batteries, and particularly discloses a composition for promoting continuous production of a vanadium electrolyte and application of the composition. The invention discloses a composition for promoting continuous production of a vanadium electrolyte, which is characterized by specifically comprising the following components in parts by weight: 32-42 parts of concentrated sulfuric acid, 0.8-1.2 parts of a sulfonic acid compound, 0.05-0.15 part of sodium sulfate and 60-66 parts of water, the sulfonic acid compound is selected from any one or more of methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, sulfamic acid and p-toluenesulfonic acid. When the composition provided by the invention is used in continuous production of the vanadium electrolyte, the use amount of concentrated sulfuric acid is reduced, vanadium pentoxide can be effectively activated, and the reaction rate is increased; and the produced vanadium electrolyte has excellent electrochemical performance.

The invention relates to a perovskite solar cell based on 2-(diethanolamine) ethanesulfonic acid modification and a preparation method and application thereof. The perovskite solar cell sequentially comprises a conductive substrate, a tin dioxide electron transport layer, a 2-(diethanolamine) ethanesulfonic acid (EBS) interface passivation layer, a perovskite light absorption layer, a hole transport layer and a metal electrode layer from bottom to top. Compared with the prior art, the EBS interface passivation layer is constructed between the stannic oxide electron transport layer and the perovskite light absorption layer, so that the dual passivation effect is achieved; the oxygen vacancy defect is passivated through tight combination of a hydroxyl group and the stannic oxide layer; the sulfonic acid group is combined with the perovskite layer to passivate Pb and I ion defects, so that the conductivity of the electron transport layer is improved, energy level matching is promoted, the loss caused by non-radiative recombination is reduced, the residual stress of the perovskite layer is released, and the overall performance of the device is improved.

The invention provides an oral thylakoid product for resisting organ failure and aging, which is prepared by the following steps: extracting thylakoid from plants including spinach, arabidopsis thaliana, lettuce, cabbage, green algae or wheat seedlings, redissolving the thylakoid into a buffer salt solution A, and carrying out ultrasonic treatment for 10-20 minutes under the ultrasonic power of 200-500W to prepare a suspension; adjusting the concentration of the suspension to 0.5-2 mg / ml, and obtaining a nano capsule-like body with the particle size of 200-500 nm by using a microfluidic technology; the buffer salt solution A is prepared from 10 to 100 mmol / L of 4-hydroxyethyl piperazine ethanesulfonic acid (HEPES), 1 to 2 mmol / L of ethylenediamine tetraacetic acid (EDTA) disodium, 0.5 to 4 mmol / L of magnesium chloride (MgCl2), 1 to 10 mmol / L of ascorbic acid and 100 to 500 mmol / L of sapoeda sodium (SNAC), and the pH value of the buffer salt solution A is adjusted to 7.5 to 8.0; and the concentration of the capsule in the buffer salt solution A is 100-400 mg / mL. According to the product, self QPRT enzyme of the thylakoid is used for efficiently catalyzing de novo synthesis of NAD + of whole body tissues and organs, so that multiple physiological processes such as energy synthesis enhancement, oxidative stress reduction and inflammatory reaction relief are finally realized, organ failure is efficiently reversed, and organ aging is relieved.

The invention provides a preparation method of a nano-gold star material, which comprises the following steps: stirring and heating a chloroauric acid solution, adding a sodium citrate solution, continuously stirring and heating, when the color of the solution is changed from colorless to dark red, adjusting the rotating speed to 600rpm, turning off a heating device, cooling at room temperature, filtering the reacted solution by using a water-based filter membrane, and drying to obtain the nano-gold star material. Removing impurities to obtain a gold seed solution; characterizing the gold seed solution and the gold seed particles; the preparation method comprises the following steps: adding a 4-hydroxyethyl piperazine ethanesulfonic acid solution, a gold seed solution, a hydroxylamine solution and a magnetic rotor into deionized water, dropwise adding a 22.5 mM chloroauric acid solution, adjusting the rotating speed to 700rpm, and reacting for 15 minutes at room temperature to obtain the nano gold star material. The prepared nano gold star material is uniform, stable, adjustable in particle size and high in dispersity, the material is in a three-dimensional multi-branch shape, and the diameter of the material is adjustable within 50-200 nm.

The invention discloses an anti-oxidation, whitening and brightening composition as well as a preparation method and application thereof. The anti-oxidation, whitening and brightening composition is prepared from the following components in parts by weight: 2 to 6 parts of nicotinamide, 0.5 to 5 parts of tranexamic acid, 1 to 5 parts of nonapeptide-1 solution, 0.5 to 1 part of tetrahydromethylpyrimidine carboxylic acid, 0.5 to 2 parts of pink red plum compound essence, 0.01 to 0.5 part of hydroxyethyl piperazine ethanesulfonic acid, 0.01 to 0.5 part of glabridin and 0.0001 to 0.2 part of glutathione. According to the invention, the composition prepared from nicotinamide, tranexamic acid, nonapeptide-1, tetrahydromethylpyrimidine carboxylic acid, pink-red plum composite essence, hydroxyethyl piperazine ethanesulfonic acid, glabridin and glutathione is prepared into the essence, and the clearance rate of the essence to ABTS free radicals reaches 58% or above, which shows that the essence has very good antioxidant ability; the cell tyrosinase activity inhibition rate of the composition under the concentration of 1.2% (v / v) reaches 51% or above, which shows that the composition has very good whitening and brightening effects.

The application discloses an anticoagulant biomaterial for blood purification and a preparation method and application thereof, and comprises the following steps: providing an amphiphilic copolymer P(AA-MMA); blending polyethersulfone, P(AA-MMA) and a solvent, stirring at a set temperature to obtain a casting solution, and preparing a blending membrane from the casting solution; soaking the blending membrane in a 2-morpholine-4-yl ethanesulfonic acid buffer solution containing argatroban, 1-ethyl-(3-dimethylaminopropyl) carbonyl diimide and hydroxy succinimide, and reacting at room temperature to obtain an argatroban grafted blending membrane, namely the anticoagulant biomaterial. The method improves the hydrophilicity of the membrane, reduces the albumin adsorption of the membrane, reduces the adhesion of platelets on the membrane, prolongs the coagulation time APTT, PT and TT of the membrane, and is better in biocompatibility and enhanced in anticoagulation performance.

This invention provides a fluorescence-enhanced composite probe based on gold nanoclusters anchored on a zinc-based coordination polymer, its preparation method, and its application, belonging to the field of probe preparation technology. The method involves first mixing an aqueous solution of glutathione and an aqueous solution of tetrachlorohydrin to obtain a gold nanocluster solution; then adding adenine and water to a 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid buffer solution and stirring until homogeneous, followed by the addition of a zinc chloride solution to obtain a zinc-based coordination polymer; finally, the gold nanocluster solution and the zinc-based coordination polymer solution are mixed homogeneously to obtain the fluorescence-enhanced composite probe based on gold nanoclusters anchored on the zinc-based coordination polymer. The probe of this invention can rapidly and sensitively recognize adenosine triphosphate (ATP). It can also be used to construct fluorescent hydrogels by mixing this material with κ-carrageenan; the hydrogels exhibit a sensitive response to ATP and good self-healing ability.

The application belongs to the technical field of oil exploitation, and particularly relates to an acid liquid for acid treatment of an oil layer and a preparation method thereof. The preparation method is as follows: the specific steps of the preparation method are as follows: in a reactor, piperazine-1,4-diethyl sulfonate monosodium salt, ethanol and bromoalkane are added, heated to reflux, and during the reaction, sodium hydroxide solution is used to maintain pH 7-8; 1-bromo-6-(trimethylammonium) hexyl bromide is added, heated to reflux, and during the reaction, sodium hydroxide solution is used to maintain pH 8-9; vacuum distillation is performed to obtain a viscous solid, cyclohexane recrystallization is performed to obtain a solid, and the solid is dried at 90 DEG C overnight to obtain corrosion-inhibiting acid; hydrochloric acid, hydrofluoric acid, hydroxyethylidene diphosphonic acid, corrosion-inhibiting acid and water are added to the reactor, stirred uniformly, and the acid liquid is obtained. The acid liquid has the advantages of low corrosion rate and long acidification time.

The invention relates to an isotropic liquid composition comprising acylisethionate, methylacyltaurate, and an amphoteric, zwitterionic, and / or nonionic surfactant. Unexpectedly, it has been found that monitoring the ratio of isethionate to taurate results in improved foaming.

The invention relates to an establishment method and application of a micropterus salmoides muscle cell line, and belongs to the technical field of cell culture. The invention provides a culture solution which takes an M199 culture medium as a basic culture medium and further comprises sodium pyruvate, hydroxyethyl piperazine ethanesulfonic acid, beta-mercaptoethanol, penicillin, streptomycin and fetal calf serum. The culture solution provides optimal conditions for micropterus salmoides muscle cell culture, the culture cost is also reduced, the micropterus salmoides muscle cell line is established by adopting the culture solution, the method is simple and easy to operate and high in repeatability, the established cell line is good in stability, continuous passage and ultralow-temperature cryopreservation can be realized, the cell division speed is high, the passage time is short, the adherence rate is high, and the method is suitable for large-scale popularization and application. In the method, the micropterus salmoides muscle primary cell culture time is short, the number of obtained cells is large, the activity is good, and passage can be carried out in only 8 days.

The application discloses a kind of high elasticity organosilicon aerogel and preparation method thereof, belong to porous material technical field;Including the following steps: S1, aldehyde group carboxymethyl cellulose is added in morpholine ethanesulfonic acid buffer solution stirring dissolution, add morpholinium, stirring reaction, add 3-amino phenylboronic acid, continue stirring reaction, washing dialysis, freeze drying, and obtain modified carboxymethyl cellulose;S2, organosilicon precursor is added in deionized water stirring uniform, add modified carboxymethyl cellulose, modified inorganic filler continues stirring reaction, add acid and adjust pH and carry out hydrolysis reaction, and obtain sol;S3, to sol is added in alkali and adjust pH, stirring uniform, after standing, and obtain gel;S4, the gel is freeze-dried, and obtain high elasticity organosilicon aerogel;The preparation method process is simple, cost is relatively low, suitable for large-scale production, and the obtained organosilicon aerogel is excellent and mechanical property and low thermal conductivity, and has good application prospect.

The application discloses a kind of on-line double-gradient pre-concentration detection methods of soil heavy metal ions based on microchip electrophoresis non-contact conductivity detection, including preparation of the analyte sample to be measured, preparation of high concentration buffer as background buffer, preparation of microchip, microchip electrophoresis process and the like steps.When testing, the background buffer contains 2-(N-morpholinyl)ethanesulfonic acid, L-histidine and electroosmotic flow inhibitor;The analyte sample to be measured contains sample matrix liquid and sample extraction stock solution.Among them, the sample matrix liquid is low-concentration buffer, and glacial acetic acid is used to reduce pH value.In testing, due to the existence of concentration gradient and pH gradient between the analyte sample solution to be measured and the background buffer, the ions to be measured in the sample will slow down and accumulate at the contact surface of the two during migration, realizing on-line pre-concentration, and improving the detection sensitivity of the ions to be measured.The application greatly improves the separation degree of multiple heavy metal ions, and effectively improves the detection sensitivity of heavy metal ions.

This invention provides a highly adhesive, wide-temperature-range aqueous hydrogel electrolyte for zinc-ion batteries, its preparation method, and its applications. The method includes the following steps: dissolving sodium alginate in 2-morpholine ethanesulfonic acid buffer solution, adding EDC and NHS, and activating; then adding dopamine hydrochloride, undergoing an amidation reaction, dialysis purification, and freeze-drying to obtain SA-DA powder; adding gelatin and SA-DA powder to deionized water, stirring and mixing evenly, and allowing to stand; then immersing in the electrolyte and undergoing soaking treatment to obtain the final product. The electrolyte of this invention exhibits excellent interfacial adhesion and flexible mechanical properties, significantly improved electrochemical stability and cycle life, excellent wide-temperature-range adaptability and anti-drying ability, outstanding safety and biocompatibility, and a simple and highly controllable process, providing key technical support for the development of high-performance, high-safety flexible aqueous zinc-ion batteries.

The invention relates to a culture method and a culture medium for reducing the number of differential transcripts of epithelial organoid and in-vivo tissues, and solves the technical problems of low growth rate and low formation rate of the organoid in the prior art. The basic components of the culture medium comprise a DMEM / F12 basal culture medium, a penicillin-streptomycin solution, a Glutamax additive, a recombinant spinal protein, a recombinant head protein, Y-27632, an epidermal growth factor, an N-2-hydroxyethyl piperazine-N-2-ethanesulfonic acid buffer solution, nicotinamide, A83-01 and CHIR99021, and the additional components of the culture medium comprise a Wnt-3A additive, an ITS-X additive and hydrocortisone. The invention also provides a culture method of the epithelial organoid. The method can be used for culturing epithelial organs.

The present application provides a kind of nintedanib solution for inhalation and preparation method and application, the inhalation solution includes active substance, solvent and auxiliary material, the single dose specification of the inhalation solution is 1-5ml, and the active substance in single dose specification is 2.41-12.04mg / ml nintedanib ethanesulfonic acid;The solvent is sterile injection solution water;The auxiliary material includes isotonicity regulator and pH regulator and can also include antioxidant;The addition amount of the isotonicity regulator is to make the inhalation solution have the weight molal osmotic concentration of 240-400mOsmol / kg;The addition of the pH regulator is to make the pH value of the inhalation solution preparation 3.0-4.0.The inhalation nintedanib solution of the present application can provide a kind of nintedanib for inhalation with convenient, safe and effective, and good stability of drug dosage form and drug delivery system in combination with liquid atomization device.

The application belongs to the technical field of nintedanib preparation development, and particularly relates to a nintedanib suspension preparation, a preparation method thereof and application of the nintedanib suspension preparation in treatment of pulmonary fibrosis. A phospholipid material is dissolved in an organic solvent under the condition of 50-70 DEG C to obtain solution A; an inorganic salt is dissolved in water for injection to obtain solution B; nintedanib ethanesulfonic acid is dissolved in water for injection to obtain solution C; an osmotic pressure regulator is dissolved in water for injection to obtain solution D; solution A and solution B are mixed by microfluidic to obtain suspension E; the organic solvent in the suspension E is removed by tangential flow membrane filtration, then solution C is added and kept for a period of time, and solution D is added as needed and mixed; the suspension can be filtered by a sterilization filter to obtain the nintedanib suspension preparation. The nintedanib prepared in the application is in a stable physical state, has excellent slow-release effect, is suitable for low-dose inhalation administration in the lung, and is helpful to improve the treatment effect and reduce side effects.

This invention relates to the field of gastric cancer organoid microarray technology, and more particularly to a gastric cancer organoid microarray culture medium and its applications. The invention provides a novel culture medium adapted for gastric cancer organoid microarray culture. The medium components include basal medium, penicillin / streptomycin dual antibiotics, antibacterial agents, hydroxyethylpiperazine ethanesulfonic acid, B-27 additive, Glutamax culture medium additive, R-vertebral protein 1, scutellarin B, and tetramethylpyrazine, among other components. This invention solves key problems in existing technologies such as slow growth, morphological instability, poor heterogeneity adaptation, and low long-term culture activity of gastric cancer organoids, providing an ideal experimental model for high-throughput screening and precision medicine applications on microarrays.

The invention belongs to the technical field of oil exploitation, and particularly relates to an oil-displacing agent for improving recovery efficiency and a preparation method of the oil-displacing agent. The preparation method comprises the following steps: (1) sequentially adding 2-[(tri (hydroxymethyl) methyl) amino]-1-sodium ethanesulfonate, DMF (Dimethyl Formamide) and bromoalkane into a first reactor, carrying out a heat preservation reaction, and maintaining the pH value of a mixed solution to be 8-9 by using a sodium hydroxide solution during the reaction; carrying out reduced pressure distillation on the reaction liquid until the reaction liquid is dry to obtain a viscous solid, transferring the viscous solid into a high-pressure reaction kettle by using toluene, adding a catalyst, purging the reaction kettle by using nitrogen, vacuumizing, introducing epoxypropane, and heating for reaction; and after the reaction is finished, transferring the mixed solution into a second reactor, adding 1 mol / L of DMF solution of sodium chloroacetate, heating and refluxing, and carrying out reduced pressure distillation on the mixed solution to obtain the product oil-displacing agent. The oil-displacing agent disclosed by the invention has the advantages of low interfacial tension and good emulsification and viscosity reduction effects.

The invention relates to a nitric oxide donor based on heparin sodium and a preparation method and application thereof, the method comprises the following steps: (1) dissolving heparin sodium in a 2-(N-morpholino) ethanesulfonic acid buffer solution, adding an activator, and carrying out a reaction to obtain a reaction solution A; (2) adding beta-mercaptoethylamine into the reaction solution A, and continuously reacting to obtain a reaction solution B; (3) dialyzing the reaction solution B to obtain a solution C; and (4) adding tert-butyl nitrite into the solution C, and reacting to obtain the heparin sodium-based nitric oxide donor. Wherein in the step (1), the activating agent is 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide or hydrochloride of the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. According to the prepared heparin sodium-based nitric oxide donor, the nitric oxide release time is prolonged, and the problem that the half-life period of nitric oxide is short is solved.

The invention provides a glycocholic acid determination kit. The glycocholic acid determination kit comprises a reagent R1 and a reagent R2, the reagent R1 is prepared from the following components: a 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution, sodium chloride, a blocking agent, CG-BSA, a surfactant and Pc300; the reagent R2 is prepared from the following components: a 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution, latex particles coated with glycocholic acid antibodies, a suspending aid and Pc300. The kit is a liquid kit which is strong in stability, high in accuracy, high in sensitivity, wide in linear range and anti-interference.

The present invention relates to the technical field of antioxidant technology for livestock sperm, and provides a highly efficient antioxidant donkey sperm diluent, a preparation method thereof, and an application. A highly efficient antioxidant donkey sperm diluent includes a basal medium and additives, wherein: the basal medium contains the following components by weight in every 1000 mL of water: 30 - 60 g of glucose, 4 - 6 g of 4 - (2 - hydroxyethyl)piperazine - 1 - ethanesulfonic acid, 12 - 24 g of skim milk powder, and 0.5 - 1.1 g of potassium citrate; the additives contain the following components by weight in every 1000 mL of the basal medium: 5 mg - 10 mg of sulforaphane, 0.1 mg - 0.2 mg of stigmasterol, 40 μg - 100 μg of enrofloxacin concentrate, and 3 mg - 5 mg of cefotaxime sodium. The present invention can effectively improve the conception rate of female donkeys, reduce the incidence of metritis, and improve the in vitro sperm motility.

The present application relates to a kind of heparin sodium-based nitric oxide donor and its preparation method and application, the method includes the following steps: (1) heparin sodium is dissolved in 2-(N-morpholino) ethanesulfonic acid buffer solution, adding activating agent, reaction is carried out, and reaction liquid A is obtained;(2) to reaction liquid A, continue to react to obtain reaction liquid B by adding β-mercaptoethylamine;(3) reaction liquid B is dialyzed, and solution C is obtained;(4) to solution C, nitrous acid tert-butyl ester is added, and the heparin sodium-based nitric oxide donor is obtained by reaction;Wherein, in step (1), the activating agent is 1-ethyl-3-(3-dimethylaminopropyl) carbonyl diimide or its hydrochloride salt.The heparin sodium-based nitric oxide donor prepared in the present application prolongs the release time of nitric oxide, and solves the problem of short half-life of nitric oxide.

The application discloses a kind of loose sandstone gas reservoirs gel particles for water plugging and a preparation method thereof, and relates to the technical field of preparation process of gel particles for water plugging.The existing gel particles after water absorption network structure has the problems of large brittleness, easy to break, poor long-term stability, and the total weight is 100 parts, including the following components:12-18 parts of acrylamide monomer, 1-3 parts of 2-acrylamido-2-tetradecyl ethanesulfonic acid, 1-3 parts of sepiolite, 0.5-2 parts of hydrophilic fiber, 0.12-0.18 parts of N-hydroxymethyl acrylamide, 0.015-0.06 parts of initiator, and the balance is water;The 2-acrylamido-2-tetradecyl ethanesulfonic acid used is a branched salt-tolerant monomer, which is crosslinked and polymerized with sepiolite of fibrous structure and hydrophilic fiber with excellent mechanical tensile properties, so that the tensile properties and salt tolerance of the prepared gel particles are improved.

Provided are a liquefying agent and the use thereof, and a nucleic acid detection system. The liquefying agent comprises a strong base, acetylcysteine, and 2-(N-morpholino)ethanesulfonic acid. In the liquefying agent, the concentration of the strong base is 0.2-1 mol / L; the concentration of the acetylcysteine is 3-300 mmol / L; and the concentration of the 2-(N-morpholino)ethanesulfonic acid is 2-5 mmol / L. By means of the synergistic effect of the strong base, acetylcysteine and 2-(N-morpholino)ethanesulfonic acid, the liquefaction time can be significantly shortened, thereby improving the efficiency and speed of treating viscous biological samples. Furthermore, the viscous biological samples treated with the liquefying agent can be directly used for nucleic acid amplification and have a long storage period. Long-term stability ensures the accuracy and reliability of nucleic acid detection, thereby enhancing the credibility of detection results.