472 results about "Ethanesulfonic acid" patented technology

The invention provides a method for detecting urea based on a metal organic framework material fluorescent sensor. The method comprises the following steps that 1, a zirconium metal organic framework material UiO-66-NH2 is prepared; 2, the zirconium metal organic framework material UiO-66-NH2 is uniformly ground, ground powder is weighed and subjected to ultrasonic oscillating to be dispersed in distilled water, and a UiO-66-NH2 material solution is obtained and stored at 4 DEG C in a lucifugal mode for use; 3, urease is taken to be dissolved in a hydroxyethyl piperazine ethanesulfonic acid buffer solution, and a urease solution is prepared and stored at 4 DEG C in a lucifugal mode for use; 4, the UiO-66-NH2 material solution and the urease solution are taken separately, fully mixed and kept for 10 min at 37 DEG C, and a fluorescence signal is detected; 5, urea with different concentrations is added, incubation is conducted for 30 min at 37 DEG C, and fluorescence signals in the presence of the urea with different concentrations are detected; 6, qualitative and quantitative analyses are conducted on urea in a sample according to the fluorescence signals and a working curve. The method for detecting urea based on the metal organic framework material fluorescent sensor is a urea detection method which is rapid, accurate, reliable, sensitive and high in specificity.

The invention discloses a viscoelastic weak gel profile control agent which is applied in oil field and oil well water injection operation. The viscoelastic weak gel profile control agent comprises the following components in parts by weight: 0.15-0.25 part of viscoelastic surface activity polymer, 0.05-0.15 part of cross-linking agent, 0.001-0.005 part of conditioning agent, and 99.8-99.6 parts of water. The viscoelastic surface activity polymer comprises the following components in parts by weight: 20-30 parts of acrylic amide, 1-2 parts of 3,3-dimethylacrylic acid, 1.5-3.5 parts of 2-acrylamido-2-dodecyl ethanesulfonic acid and/or 1-4 parts of N,N-dimethyl-allyl-hexadecyl ammonium chloride, 60-75 parts of deionized water and 0.3-1 part of initiating agent. The initiating agent comprises the following components in parts by weight: 2.5-3.5 parts of ammonium persulphate and 1 part of sodium sulfite. The viscoelastic weak gel profile control agent of the invention has a certain gel strength, capacity for lowering oil-water surface tensility and adjustable gel forming time, can improve water injection profile, lower oil-water surface tensility, wash away residual oil in a pore canal and improve water injection recovery ratio.

The invention discloses a flow cytometry-based sperm acrosome reaction detection reagent which comprises acrosome reaction induction fluid and staining fluid, as well as sperm capacitation fluid, the pH value of the sperm capacitation fluid is 7.0-7.8, the sperm capacitation fluid contains sodium chloride, potassium dihydrogen phosphate, glucose, potassium chloride, magnesium sulfate heptahydrate, calcium chloride dehydrate, N-2-piperazine-N-2-ethanesulfonic acid disodium salt, sodium bicarbonate, sodium acetone, bovine serum albumin, and sodium lactate solution, the sperm capacitation fluid replaces the current sperm culture fluid, so that the capacitation effect is good and the induction is facilitated; the sperm acrosome reaction can be detected by using flow cytometry, and the flow cytometry-based sperm acrosome reaction detection reagent is accurate in the sperm acrosome reaction detection result, stable and reliable, good in repeatability, and clinically easily popularized.

The invention discloses a high-molecular heavy metal flocculant and a preparation method thereof. The high-molecular heavy metal flocculant forms white powder in appearance, and a solution of the high-molecular heavy metal flocculant is colorless transparent liquid. The preparation method of the high-molecular heavy metal flocculant comprises the following steps of: adding 1 part of carboxymethyl cellulose and acrylamide to a reaction kettle according to a molecular weight ratio of 1 to 0.8, then adding 10 parts of water, heating to 50-60 DEG C, and introducing nitrogen under the action of mechanical stirring; keeping temperature at 50-60 DEG C, gradually adding 0.1 mol/L of initiating agents after 10 minutes, and gradually adding 0.1 mol/L of emulsifying agents after 15 minutes, and removing the nitrogen after the addition is completed for 30 minutes; continuing at constant temperature of 50-60 DEG C for 2.5 hours to prepare a polyacrylamide graft copolymer; cooling the reaction kettle to 30 degrees, adding NaOH and 2-sulfhydryl ethanesulfonic acid under the action of mechanical stirring, and carrying out amidation reaction for 3 hours under an alkaline condition, wherein the weight percentage ratio of NaOH to 2-2-sulfhydryl ethanesulfonic acid is 1:1.2. The high-molecular heavy metal flocculant disclosed by the invention can be used for treating the heavy metal ions contained in waste water and can also be used for the treatment of sludge.

The invention belongs to industrial recirculated cooling water treatment technical field, concretely relates to a biodegradable corrosion and scale inhibitor epoxy succinic acid copolymer and its preparation method. According to the invention, maleic anhydride is taken as a raw material, is subjected to alkaline hydrolysis to generate disodium maleate, under the effect of a catalyst, hydrogen peroxide is oxidized to epoxy disodium succinate; epoxy disodium succinate and acrylic acid, 3-allyloxy-2-hydroxy-1-moropholino ethanesulfonic acid monosodium salt are subjected to copolymerization to obtain epoxy succinic acid/acrylic acid/3-allyloxy-2-hydroxy-1-moropholino ethanesulfonic acid monosodium salt copolymer. The corrosion and scale inhibitor has the advantages of no phosphor, no nitrogen, high temperature resistance and biodegradable effect, and is a environment-friendly type corrosion and scale inhibitor. The prepared polymer possesses good scale inhibition performance and rust inhibition performance, and can be used for scale inhibition treatment in the fields of recirculated cooling water, boiler water, oil field water, membrane treatment and the like, and is a scale inhibition dispersant with excellent performance.

The invention discloses a NaYF4: Yb, Er/Mn up-conversion luminescence nanometer material modified by manganese dioxide nanosheet, a preparation method thereof, a detection method of hydrogen peroxide or choline and an application. The method includes steps of 1), performing contact reaction and hydrothermal reaction on erbium source, manganese source, ytterbium source, trisodium citrate, sodium fluoride, yttrium source, water, concentrated nitric acid CTAB and alcohol; washing and separating so as to acquire the NaYF4, Yb, Er/Mn up-conversion luminescence nanometer material; 2), in the presence of protection gas and organic solvent, performing ligand exchange reaction on polyacrylic acid, diethylene glycol, and NaYF4: Yb, Er/Mn so as to acquire PAA modified NaYF4: Yb, Er/Mn up-conversion luminescence nanometer material; 3), spreading the PAA modified NaYF4: Yb, Er/Mn up-conversion luminescence nanometer material at ethanesulfonic acid, and then adding potassium permanganate to perform the contact reaction, so as to acquire the NaYF4: Yb, Er/Mn modified by MnO2 nanosheet. The up-conversion luminescence nanometer material can detect hydrogen oxide or choline.

The invention relates to a neural stem cells medium and a method for performing human neural stem cells in-vitro long-term culture and amplification by using the neural stem cells medium. The neural stem cells medium comprises the following ingredients by weight proportion: 100-1000 micrograms of heparin sodium, 10-100 micrograms of vitamin E, 5-50 milligrams of insulin human recombinant, 0.5-5 milligrams of putrescine, 2-10 micrograms of sodium selenite, 2-10 milligrams of human transferrin, 2-10 micrograms of progestin, 300 milligrams of L-glutamine, 5.9 grams of 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid, 10-100 micrograms of recombinant human epidermal growth factors, 10-100 micrograms of recombinant human basic fibroblast growth factors, 20-200 milligrams of vitamin C glucoside and 40,000-400,000 IU (international unit) of gentamicin. By the neural stem cells medium, the technical problems that human neural stem cells are easy to differentiate when cultured in vitro and long-term culture and amplification are difficult to implement are solved.

The invention discloses a fluorescein rhodamine B double-Schiff alkali compound ultraviolet molecular probe for Fe<3+> detection as well as synthesis and application thereof, and relates to a Fe<3+> probe as well as synthesis and application thereof. The technical problems that an apparatus required by an existing Fe<3+> fluorescent molecular probe is expensive and the ultraviolet probe is susceptible to interference are solved. A structural formula of the ultraviolet molecular probe disclosed by the invention is that the formula (1) is shown in the description. A preparation method comprises the following steps: carrying out condensation reaction between single-imino fluorescein aldehyde and rhodamine B hydrazine, and carrying out column chromatography separation on a mixed solvent of petroleum ether and ethyl acetate to obtain the ultraviolet molecular probe; dissolving the probe into a mixed solution of DMF (N,N-Dimethyl Formamide) and an HEPES (2-[4-(2-Hydroxyethyl)-1-Piperazinyl] Ethanesulfonic Acid) buffer solution; detecting ultraviolet spectral absorbance values before and after addition of to-be-detected samples, and judging that a sample solution contains Fe<3+> if the absorbance values of parts on which the wavelengths are 323nm and 358nm are increased. The method is simple and quick.

The invention discloses a culture medium for promoting an adventive root of a woody plant to root and grow and an application of the culture medium. The culture medium comprises the following components: inorganic salt A, inorganic salt B, an organic compound, 50-150mg/L water-soluble carbon nano tubes, 0.5-1.5mg/L indolebutyric acid, 100mg/L 2-(N-morpholine) ethanesulfonic acid, 30000mg/L cane sugar and 5000mg/L agar. The culture medium provided by the invention is more capable of promoting the growth of the adventive root. The largest difficulty of asexual reproduction of the woody plant lies in the rooting and the growth of the adventive root, the culture medium has the same promotion effect for the rooting of the adventive root of brassaia actinophylla and the rooting and the growth of plants such as honeysuckle, blueberry, small kiriko, swida wilsoniana, zanthoxylum dissitum and the like.

The invention discloses a nucleic acid protective liquid and an application thereof for long-term storage and transportation of a tissue sample at constant temperature. The nucleic acid protective liquid comprises the following components: D-glucose, 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid, inorganic salt, amino acids, choline chloride, folic acid, nicotinamide, inositol, vitamin A, vitamin H, vitamin E, Holo-human transferrin, bovine serum albumin, N-(2)-L-alanyl-L-glutamine, insulin, adrenalone, linoleic acid, linolenic acid, progesterone, penicillin, streptomycin, amphotericin B and water. The nucleic acid protective liquid is low in cost. By using the nucleic acid protective liquid, nucleic acid in the tissue sample is long in storage time at room temperature, and the obtained nucleic acid is high in concentration and great in total amount. A preparation method for the nucleic acid protective liquid is simple and fast.

The invention puts forward a universal cancer organoid in-vitro culture medium, which consists of a DMEM/F-12K (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12K) basic culture medium and an adding factor, wherein the adding factor consists of HEPES (2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid), L-Glutamine, EGF (epidermal growth factor), Noggin, FGF (fibroblast growth factor)-10, A83-01 and Y27632. According to the culture medium of the embodiment of the invention, the success rate of organoid passage culture can be greatly improved, in addition, the universal culture of cancer samples, including gastric carcinoma, rectal carcinoma, lung carcinoma and the like, can be realized, culture cost is greatly lowered, the long-term culture of the organoid can be realized, and abiobank is established.

A family of slurries useful in modifying exposed surfaces of wafers for semiconductor fabrication are provided along with methods of modifying exposed surfaces of wafers for semiconductor fabrication utilizing such a family of working slurries, and semiconductor wafers. The slurries of the invention are a solution of initial components, the components comprising: a sulfonated zwitterion selected from 2-(N-Morpholino)ethanesulfonic acid, (3-[N-Morpholino])propanesulfonic acid, 2-[(2-Amino-2-oxoethyl)amino]ethanesulfonic acid, Piperazine-N, N′-bis(2-ethanesulfonic acid), 3-(N-Morpholino)-2-hydroxypropanesulfonic acid, N ,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, 3-(N-Morpholino)propanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), N-Tris(hydroxymethyl)methyl-2 aminoethanesulfonic acid, 3-[N ,N-Bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid, 3-[N -Tris(hydroxymethyl)methylamino)-2-hydroxypropanesulfonic acid, N-(2-hydroxyethyl)piperazine-N′-(2-hydroxypropanesulfonic acid), Piperazine-N ,N′-bis(2-hydroxypropanesulfonic acid), N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid, 3-[(1,1-Dimethy 1-2-hydroxyethyl)amino]-2-hydroxypropanesulfonic, acid, 2-(N-Cyclohexylamino)ethanesulfonic acid, 3-(Cyclohexylamino)-2-hydroxy-I-propanesulfonic acid, 2-Amino-2-methyl-I-propanol, 3-(Cyclohexylamino)-1-propanesulfonic acid, an oxidizing agent; optionally, a passivating agent; optionally a chelating agent, optionally abrasive particles, optionally a surfactant, optionally a secondary buffering agent and water. The method of the invention comprises the steps of: a) providing a wafer comprising a first material having a surface etched to form a pattern and a second material deposited over the surface of the first material; b) contacting the second material of the wafer with abrasive in the presence of the working slurry; and c) relatively moving the wafer or polishing pad or both while the second material is in contact with the slurry and abrasive particles until an exposed surface of the wafer is planar and comprises at least one area of exposed first material and one area of exposed second material.

The invention provides a preparation method of high-glossiness high-water-permeability polyurethane resin.Firstly, a waterborne polyurethane prepolymer is prepared, partial end capping is conducted on the prepolymer with octadecylamine, then a hydrophilic chain extender 2-[(2-amino ethyl)amino]sodium ethanesulfonic acid water solution is added dropwise while the prepolymer is dispersed in deionized water, after chain extension is conducted, then acetone or butanone in a system is removed under the vacuum condition, and the high-glossiness high-water-permeability polyurethane resin is prepared.The method is simple in process, it is unnecessary to add a large quantity of organic solvents, no defoamer is needed, and the solid content of the obtained high-glossiness high-water-permeability polyurethane resin is high and is 41-46%; the tensile strength is 39.8-42.2 MPa, and the elongation at break is 789-857%.Glossiness (60 degrees) is 90, light transmittance is 89%, qualification is good, and adhesive film is good in water resistance and solvent resistance and high in mechanical property, glossiness and light transmittance.

The invention provides a perfusion stock composition, for preserving a donor organ for transplantation, comprising: a source of 60 to 100 mM Na⁺; a source of 10 to 20 mM K⁺; a source of 5 to 10 mM Mg²⁻; a source of 0.25 to 0.75 mM Ca²⁺; 10 to 40 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris or THAM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 3-(N-morpholino)propanesulfonic acid (MOPS), 2-(N-morpholino)ethanesulfonic acid (MES), N,/N-bis-(2-hydroxyethyl)-2-aminoethansulfonic acid (BES), or N/-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES); a source of 10 to 30 mM HCO₃⁻; 1 to 30 mM glucose; 1 to 20 U/L insulin; 1 to 10 mM fructose diphosphate or a salt thereof; 1 to 40 mM aspartate or glutamate; 1 to 10 mM adenosine, cAMP or cGMP; 1 to 10 mM reduced glutathione; and 30 to 100 mM lactobionate or mannitol; and optionally a diluent. The invention also provides a perfusion composition, a kit, a method, and a perfusion apparatus, each related to the perfusion stock composition.

Adipose tissue cryopreservation liquid at a clinical application level and a cryopreservation method. The adipose tissue cryopreservation liquid at the clinical application level comprises 15-50-mmol/L saccharose, 15-50-mmol/L mannitol injection, 1-5-mmol/L glucose injection, 1-3-mol/L adenosine injection, 0.5-5-mmol/L reduced glutathione, 1-4.5-mol/L trehalose and 0.1-1-mmol/L EDTA-2Na; and the components are mixed to form mixed liquid, and the mixed liquid is the adipose tissue cryopreservation liquid at the clinical application level. By means of the adipose tissue cryopreservation liquid,in combination with the cryopreservation method thereof, at the clinical application level, the defect of adverse reaction due to 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), phenol red, DMSO, animal serum, blood platelet extracts and the like which are used in the prior art is effectively overcome.

The invention discloses antibacterial gel and a preparation method thereof, and belongs to the field of gel preparation. The antibacterial gel comprises deacylation gellan gum, nisin, 2-(N-morpholine) ethanesulfonic acid, 1-(3-dimethylamino propyl)-3-ethyl carbodi-imide hydrochloride, N-hydroxy succinimide thiosulfate and deionized water. The preparation method includes the steps: resin pretreatment; solution preparation; activation; acylation; dialysis; gel forming. The deacylation gellan gum is covalently grafted to the nisin to form the antibacterial gel, so that the antibacterial gel has the advantages the antibacterial gel is high in grafting ratio, low in cost, easy to form, easily formed, thermally reversible, free from toxicity and the like, and the antibacterial gel is suitable for preparation of antibacterial washing gel.

This invention provides pH buffered plant nutrient compositions, methods for fertilizing a plant growing or a seed germinating in a hydroponics system, methods for growing a plant in a hydroponics system, and methods for making a pH buffered plant nutrient composition. The compositions and methods of this invention are useful with distilled water, deionized water, filtered water, and United States municipal tap water. The compositions and methods of this invention are useful with most of the municipal water supplies in the United States. pH buffering agents useful in the practice of this invention include phosphate buffers, aquarium buffers, 2-[N-morpholino]ethanesulfonic acid, and mixtures thereof.

The invention provides a composite acid catalyst for esterification of biodiesel. The composite acid catalyst consists of acid and an inhibitor in mass ratio of 1:(0.01-0.15), wherein the acid is methanesulfonic acid, ethanesulfonic acid or chlorosulfonic acid; the inhibitor is phosphomonoesterase, polyether acid phosphate or propargyl alcohol. The catalyst is applied to the esterification reaction of biodiesel, causes less dosage, has high catalyzing activity, does not produce other byproducts during the reaction, brings low requirement on material of equipment, and can be used for effectively solving the problems in esterification production of biodiesel that the catalyzing efficiency is relatively low, high requirements are brought to the materials of reactors and reaction equipment, and the products are difficult to separate.

The invention relates to a clenbuterol immunomagnetic bead separation and enrichment kit. The clenbuterol immunomagnetic bead separation and enrichment kit comprises a magnetic bead which is coupled to a clenbuterol monoclonal antibody, reconstitution fluid and a magnet, wherein the magnetic bead which is coupled to the clenbuterol monoclonal antibody is formed by mixing and dissolving the clenbuterol monoclonal antibody and the magnetic bead in 2-(N-morpholino) ethanesulfonic acid monohydrate in a coupling way according to a mass ratio of 1:(500 to 1000); the clenbuterol monoclonal antibody is obtained by taking a coupling substance obtained from clenbuterol haptin and bovine serum albumin as an immunogen to immune Balb/c mice; the clenbuterol haptin is obtained by carrying out diazo-reaction on clenbuterol and P-hydroxybenzene propanoic acid and bringing a carboxyl spacer arm on amino. The invention also relates to a sample preprocessing method for separating the clenbuterol by adopting the clenbuterol immunomagnetic bead separation and enrichment kit. According to the sample preprocessing method disclosed by the invention, higher specificity, higher recovery rate and higher accuracy on the clenbuterol are obtained, sample preprocessing steps are simplified, and various and a mass of organic solvents can be prevented from being used during a sample preprocessing procedure.