Culture medium for promoting adventive root of woody plant to root and grow and application of culture medium

A woody plant and culture medium technology, applied in the field of special medium, can solve the problems that affect the development and application of tissue culture and rapid propagation of woody plants, woody plants are not suitable, and are prone to browning, so as to prevent and control physiological dehydration , Avoid pH fluctuations and accelerate absorption

Active Publication Date: 2013-06-05
JIANGSU SUMEILUN INTELLIGENT TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, MS (Murishige and Skoog, 1962) basal medium can be used for tissue culture propagation of most herbaceous plants, but MS basal medium is not suitable for many woody plants
The adventitious buds of woody plants are prone to browning, vitrification and rooting difficulties on MS medium, which seriously affects the development and application of tissue culture and rapid propagation of woody plants

Method used

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  • Culture medium for promoting adventive root of woody plant to root and grow and application of culture medium
  • Culture medium for promoting adventive root of woody plant to root and grow and application of culture medium
  • Culture medium for promoting adventive root of woody plant to root and grow and application of culture medium

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Embodiment Construction

[0023] The medium for plant adventitious root generation and growth of the present invention is mainly obtained by the following method: take the preparation of 1L medium as an example: get 600 ml of deionized water, first add inorganic salt A (ammonium nitrate, phosphoric acid) shown in Table 1 Potassium dihydrogen, potassium sulfate, calcium nitrate, magnesium sulfate), inorganic salt B shown in Table 2 (zinc nitrate hexahydrate, manganese sulfate monohydrate, boric acid, copper sulfate pentahydrate, Na 2 -EDTA, ferric sulfate heptahydrate, silicic acid, sodium molybdate, nickel sulfate hexahydrate) stirring to dissolve, then add 30000 mg / L sucrose, 100 mg / L 2-(N-morpholine) ethanesulfonic acid, 50 , 100, or 150 mg / L water-soluble carbon nanotubes (commercially available) and 5000 mg / L agar after heating and dissolving, make up to 1L with deionized water, and adjust the pH value to 5.8 with 1M KOH. Put the configured plant regulator into a 2L triangular bottle, seal it, and ...

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Abstract

The invention discloses a culture medium for promoting an adventive root of a woody plant to root and grow and an application of the culture medium. The culture medium comprises the following components: inorganic salt A, inorganic salt B, an organic compound, 50-150mg/L water-soluble carbon nano tubes, 0.5-1.5mg/L indolebutyric acid, 100mg/L 2-(N-morpholine) ethanesulfonic acid, 30000mg/L cane sugar and 5000mg/L agar. The culture medium provided by the invention is more capable of promoting the growth of the adventive root. The largest difficulty of asexual reproduction of the woody plant lies in the rooting and the growth of the adventive root, the culture medium has the same promotion effect for the rooting of the adventive root of brassaia actinophylla and the rooting and the growth of plants such as honeysuckle, blueberry, small kiriko, swida wilsoniana, zanthoxylum dissitum and the like.

Description

technical field [0001] The invention belongs to the field of plant growth, and in particular relates to a special culture medium, which promotes the occurrence and growth of plant adventitious roots and its application in rooting. technical background [0002] Adventitious roots are roots that form on non-root tissues of plants (leaves, shoots, branches, aboveground stems, underground stems, etc.). There are two ways of occurrence: direct generation and indirect generation: direct generation refers to the direct formation of adventitious roots from cuttings (stems, branches, leaves, etc.) during vegetative propagation of cuttings; Differentiate to produce adventitious roots, or in the process of tissue culture, callus tissue is first produced from explants, and then pseudo-meristems are formed to produce adventitious buds, then adventitious root primordia, and finally adventitious roots. Different types of plants, such as woody and herbaceous, monocotyledonous and dicotyled...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈建军许志赵杰
Owner JIANGSU SUMEILUN INTELLIGENT TECH
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