Culture medium for promoting adventive root of woody plant to root and grow and application of culture medium

Abstract

The invention discloses a culture medium for promoting an adventive root of a woody plant to root and grow and an application of the culture medium. The culture medium comprises the following components: inorganic salt A, inorganic salt B, an organic compound, 50-150mg / L water-soluble carbon nano tubes, 0.5-1.5mg / L indolebutyric acid, 100mg / L 2-(N-morpholine) ethanesulfonic acid, 30000mg / L cane sugar and 5000mg / L agar. The culture medium provided by the invention is more capable of promoting the growth of the adventive root. The largest difficulty of asexual reproduction of the woody plant lies in the rooting and the growth of the adventive root, the culture medium has the same promotion effect for the rooting of the adventive root of brassaia actinophylla and the rooting and the growth of plants such as honeysuckle, blueberry, small kiriko, swida wilsoniana, zanthoxylum dissitum and the like.

Description

technical field [0001] The invention belongs to the field of plant growth, and in particular relates to a special culture medium, which promotes the occurrence and growth of plant adventitious roots and its application in rooting. technical background [0002] Adventitious roots are roots that form on non-root tissues of plants (leaves, shoots, branches, aboveground stems, underground stems, etc.). There are two ways of occurrence: direct generation and indirect generation: direct generation refers to the direct formation of adventitious roots from cuttings (stems, branches, leaves, etc.) during vegetative propagation of cuttings; Differentiate to produce adventitious roots, or in the process of tissue culture, callus tissue is first produced from explants, and then pseudo-meristems are formed to produce adventitious buds, then adventitious root primordia, and finally adventitious roots. Different types of plants, such as woody and herbaceous, monocotyledonous and dicotyled...

AI Technical Summary

Problems solved by technology

For example, MS (Murishige and Skoog, 1962) basal medium can be used for tissue culture propagation of most herbaceous plants, but MS basal medium is not suitable for many woody plants

The adventitious buds of woody plants are prone to browning, vitrification and rooting difficulties on MS medium, which seriously affects the development and application of tissue culture and rapid propagation of woody plants

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