9results about How to "Use low concentration" patented technology

The invention provides an anti-biofilm composition based on the synergistic effect of amino fullerene and vancomycin and application of the anti-biofilm composition. The mass ratio of the amino fullerene to the vancomycin is 10: 1. The amino fullerene derivative and vancomycin are combined to have a remarkable synergistic effect in the aspect of inhibiting the formation of the biological membrane for the first time, and the inhibition rate of the biological membrane reaches 93%, so that the amino fullerene derivative and vancomycin have a strong synergistic effect. The composition provided by the invention can effectively remove more than 40% of formed mature biological membranes (24-48-hour biological membranes), which is an effect which cannot be realized by independent use of vancomycin.

The application relates to the technical field of cell culture, in particular to application of Gremlin1 protein in promoting proliferation of adipose-derived cells. The application also provides a culture medium containing the Gremlin1 protein, which is used for culturing the adipose-derived cells, can significantly promote proliferation of the adipose-derived cells, and is significantly superior to a commercial special culture medium.

The invention discloses a plant immune induced resistance protein Ps131903 and application thereof. The amino acid sequence of the immune induced resistance protein Ps131903 is shown as SEQ ID NO.2. The protein Ps131903 can reduce the harm of phytophthora sojae and pythium ultimum to soybeans, reduce the harm of phytophthora capsici to chilies and tomatoes and reduce the harm of pyricularia grisea to rice. The compound has the advantages of low use concentration, quick effect and broad spectrum, and provides a new way for improving the plant resistance, thereby having wide application prospects in agricultural production.

The invention relates to the field of petroleum drilling engineering, and discloses a plugging agent comprising an inorganic material with hydroxyl on the surface and a copolymer grafted on the surface of the inorganic material, a preparation method of the plugging agent and application of the plugging agent in plugging slurry for petroleum exploitation. The plugging agent is simple in preparation method and relatively low in cost, can be used for deep well and ultra-deep well operation, remarkably reduces the pressure transmission effect of well drilling, maintains relatively good well wall stability, effectively solves the problems of well wall instability, reservoir pollution and the like in well drilling work, improves economic benefits, reduces the occurrence rate of underground complex accidents, and has good application prospects. Guarantee is provided for the safety of drilling operation, and the application prospect is very wide.

This invention provides a method for preparing stem cells with enhanced osteogenic differentiation capacity, belonging to the field of biological technology. This invention significantly improves the osteogenic differentiation capacity of bone marrow mesenchymal stem cells (BMSCs) by combining deer antler polypeptide with rhodioloside. The osteogenic induction culture medium prepared in this invention includes DMEM / F12 medium, 10 mM β-glycerophosphate sodium, 50 μg / ml ascorbic acid, 100 nM dexamethasone, 20 μg / ml deer antler polypeptide, and 20 μg / ml rhodioloside. This optimized culture medium achieves a significant osteogenic promoting effect at lower concentrations, demonstrating the synergistic effect of deer antler polypeptide and rhodioloside. Simultaneously, the synergistic effect reduces the amount of components used, lowers the cost of using deer antler polypeptide, and reduces the side effects associated with high doses.

The invention discloses a silicon surface cleaning agent as well as a preparation method and application thereof. The silicon surface cleaning agent is prepared from the following raw materials in percentage by mass: 3 to 10 percent of composite acid, 0.1 to 0.5 percent of chelating agent, 2 to 3 percent of ethanol, 0.1 to 1 percent of DMSO (Dimethylsulfoxide), 1 to 3 percent of surfactant, 0.1 to 0.3 percent of metal cleaning aid and the balance of deionized water. The composite acid is composed of inorganic acid and organic acid according to the mass ratio of (2-5): (1-5). According to the invention, efficient and specific removal of potassium and sodium ions is realized, and the defect of the traditional cleaning process in the aspect is overcome; meanwhile, the cleaning agent also has an excellent cleaning effect on organic oil stains and other multiple metal ions, the process is simple and friendly, and the cost is low.

The application discloses application of 7-acetyl-lushanrubescensin A in preparation of anti-inflammatory cosmetics or medicines, and a composition containing the same and a preparation method, and belongs to the field of natural medicine research and development. The compound 7-acetyl-lushanrubescensin A is separated from isodon lichiangensis, can significantly inhibit release of NO and IL-6 inflammatory factors of RAW264.7 cells induced by LPS, and has better effect than a positive control dexamethasone in a concentration range of 1-3 mu g / mL, and has obvious anti-inflammatory activity. The 7-acetyl-lushanrubescensin A compound can be used as an anti-inflammatory active agent in skin inflammation care and / or inflammation repair cosmetics or medicines, and provides a new natural active ingredient source for care of inflammatory skin.

The application discloses an organic annulus protection fluid for oil and gas wells and a preparation method thereof. The annulus protection fluid comprises 0.01-3wt% of active polymer, 0.1-5wt% of oxygen scavenger, 0.1-2.5wt% of bactericide, 0.1-0.5% of pH regulator, and the rest is water. The active polymer in the annulus protection fluid is copolymerized from N-acryloyl morpholine and acrylate compounds. The annulus protection fluid has good chemical stability, can effectively delay the corrosion rate of the casing and the oil and gas pipeline, solve the problems of fouling, bacterial reproduction, under-deposit corrosion and plugging caused thereby, and reduce the pressure difference between the oil and gas pipeline and the annulus. The system is suitable for annulus protection of oil and gas wells, and is especially suitable for high-acid and high-sulfur oil and gas wells.

The invention provides a device and a method for preparing a surfactant containing nano bubbles and electronic equipment, and belongs to the technical field of nano bubble preparation. The device comprises: a generation system comprising a reaction vessel for preparing the surfactant containing the nano-bubbles; the temperature control system is connected with the generation system and used for maintaining the temperature of the generation system; the gas storage system is connected with the generation system and is used for providing gas and pressure required by the generation system; the ultrasonic system is connected with the generating system and used for providing ultrasonic vibration for the generating system; and the control system is configured to store preparation steps and control actions of the temperature control system, the gas storage system and the ultrasonic system based on the preparation steps. According to the invention, the interfacial tension between the displacement system and crude oil can be greatly reduced only by using a small amount of surfactant, and when the displacement system with ultralow interfacial tension is used for oil reservoir development, the oil recovery efficiency can be improved and the development cost can be reduced.