21results about How to "Use low concentration" patented technology

The invention belongs to the technical field of agriculture, and particularly relates to application of an astragalus extract containing astragalus polysaccharide in promoting plant growth. Purified astragalus polysaccharide is proved to have good growth regulation and stress resistance enhancement functions on plants, but the extraction and purification process of the polysaccharide is high in cost and low in efficiency, the requirement of large-scale production is difficult to meet, and the application of the astragalus polysaccharide in agriculture is limited. The invention provides an application of an astragalus extract containing astragalus polysaccharide, and particularly relates to an application of the astragalus extract as a plant growth promoter for promoting plant growth and improving stress resistance of plants, reducing the use concentration of the astragalus polysaccharide, promoting the application of the astragalus polysaccharide in agriculture, and providing a new choice for regulating and controlling plant growth in agriculture.

The invention provides a developable hydrogel. The developable hydrogel comprises a first component and a second component, the first component is maleimide group end capped multi-arm polyethylene glycol; and the second component is a sulfydryl modified iodine-containing contrast agent. The components of the hydrogel can be prepared into a neutral solution, the reaction is mild in a physiological environment, the safety is high, and the formed hydrogel is good in biocompatibility. The hydrogel disclosed by the invention does not cause a hypertonic phenomenon in vivo, and the degraded hydrophilic iodine molecule unit is easy to metabolize and exclude from the body. The use concentration of the two components of the hydrogel is low, the raw material concentration is greatly reduced, the use amount is reduced, the cost can be reduced, and the hydrogel is more friendly to a human body and has better clinical safety. The hydrogel provided by the invention can realize accurate adjustment of a wide gelation time window of 2-120s, the development value can be quantitatively regulated and controlled within 80-400Hu, the hydrogel is adaptive to application requirements of different tissues, and the degradation period of the hydrogel in vivo can be adjusted within 1-24 weeks.

The invention belongs to the technical field of oil-gas field development, and provides a phase permeability improver, a fracturing fluid system containing the phase permeability improver, and preparation methods and applications of the phase permeability improver and the fracturing fluid system. The phase permeability improver is obtained by copolymerization and hydrolysis of acrylamide (AM), a functional monomer 2-acrylamido-2-methylpropanesulfonic acid (AMPS) and cationic hydrophobic monomers A and B, the cationic hydrophobic monomer A is at least one of tetradecyl dimethyl allyl ammonium chloride, hexadecyl dimethyl allyl ammonium chloride and octadecyl dimethyl allyl ammonium chloride; the cationic hydrophobic monomer B is at least one of methacryloyloxyethyl trimethyl ammonium chloride, acryloyloxyethyl trimethyl ammonium chloride and acryloyloxyethyl dimethyl benzyl ammonium chloride; the phase permeability improver and the fracturing fluid system containing the phase permeability improver can obviously reduce the water phase permeability after fracturing construction, meanwhile, the influence on the oil phase permeability is small, the oil stabilizing and water controlling effects are obvious, and the low-permeability oil reservoir fracturing construction yield stabilizing effect can be obviously improved.

The invention relates to the field of cell biology, and discloses an exosome for displaying BMP7 on the surface as well as a preparation method and application of the exosome. The preparation method comprises the following steps: constructing a fusion gene containing a glycosylation sequence, an HA tag, a BMP7 gene, a Lamp2b gene and a connecting sequence, and the nucleotide sequence of the fusion gene is as shown in SEQ ID NO.1; inserting the fusion gene into an expression cassette of an expression vector to obtain a recombinant expression vector; transferring the recombinant expression vector into a cell which generates the exosome, and screening to obtain a stable BMP7-Lamp2b overexpression cell; and culturing the BMP7-Lamp2b overexpressed cells, collecting the supernatant of the cell culture medium, and purifying to obtain the exosome with the surface displaying BMP7. The prepared exosome with the surface displaying BMP7 can improve the medicine utilization rate and the action time, reduce the medicine use concentration and use frequency, finally reduce the production cost and reduce the infection risk caused by articular cavity injection.

The invention provides an anti-biofilm composition based on the synergistic effect of amino fullerene and vancomycin and application of the anti-biofilm composition. The mass ratio of the amino fullerene to the vancomycin is 10: 1. The amino fullerene derivative and vancomycin are combined to have a remarkable synergistic effect in the aspect of inhibiting the formation of the biological membrane for the first time, and the inhibition rate of the biological membrane reaches 93%, so that the amino fullerene derivative and vancomycin have a strong synergistic effect. The composition provided by the invention can effectively remove more than 40% of formed mature biological membranes (24-48-hour biological membranes), which is an effect which cannot be realized by independent use of vancomycin.

The application relates to the technical field of cell culture, in particular to application of Gremlin1 protein in promoting proliferation of adipose-derived cells. The application also provides a culture medium containing the Gremlin1 protein, which is used for culturing the adipose-derived cells, can significantly promote proliferation of the adipose-derived cells, and is significantly superior to a commercial special culture medium.

The invention discloses an optimization process for refining pig blood chlorhematin by using a glacial acetic acid method, which comprises the following process flows: pretreating fresh pig blood to obtain pig blood red blood cell powder; preparing a pig blood chlorhematin crude product by adopting an optimized glacial acetic acid process; washing the crude product by adopting a hydrochloric acid solution washing method to obtain a pig blood chlorhematin refined product; neutral washing; and performing vacuum drying to constant weight to obtain a heme product. The pig blood chlorhematin refined by the glacial acetic acid extraction combined hydrochloric acid solution washing method provided by the invention has the advantages of high yield, high purity, great reduction of the concentration of glacial acetic acid, low energy consumption, environmental friendliness and reduction of the production cost of enterprises, and is suitable for large-scale industrial implementation.

The application provides a combination of essential oils of Ledum palustre and Ophiopogon japonicus and application thereof in agricultural bacteriostasis, and belongs to the field of extraction and application of plant essential oils. The essential oil combination is obtained by mixing essential oils of Ledum palustre and Ophiopogon japonicus; the mass ratio of the essential oils of Ledum palustre and Ophiopogon japonicus is 1:0.5-1.5; the essential oils of Ledum palustre and Ophiopogon japonicus are obtained by using a steam distillation method respectively; the steam distillation method is specifically as follows: Ledum palustre leaves and crushed Ophiopogon japonicus roots are placed in respective distillation equipment, water is added, and then the temperature is raised to boiling, and distillation is performed for 1-3 hours, so that the essential oils of Ledum palustre and Ophiopogon japonicus are obtained respectively. The essential oil combination is derived from plants, is a natural bactericide, has good bacteriostatic effect, is safe to crops, is environment-friendly, and has a wide application prospect.

The invention belongs to the field of unconventional oil reservoir exploitation, and discloses a high interfacial tension type imbibition oil washing agent for improving shale oil reservoir recovery efficiency, which comprises the following components in percentage by mass: 0.1-0.6% of low molecular alcohol ether, 0.01-0.3% of water-soluble surfactant, 0.02-0.07% of inorganic salt and the balance of water. The shale oil reservoir oil washing agent has the advantages of good wetting reversal capability, small solution particle size, low use concentration and strong infiltration capability, the oil-water interfacial tension of the solution is 4-6mN / m, the particle size is less than 60nm, the natural shale oil reservoir rock core is converted from oil wetting (the contact angle is greater than 90 degrees) to water wetting (the contact angle is 50-60 degrees), the shale oil reservoir oil washing efficiency is greater than 45%, and the shale oil reservoir oil washing agent has wide application prospects in shale oil reservoirs.

The invention discloses a plant immune induced resistance protein Ps131903 and application thereof. The amino acid sequence of the immune induced resistance protein Ps131903 is shown as SEQ ID NO.2. The protein Ps131903 can reduce the harm of phytophthora sojae and pythium ultimum to soybeans, reduce the harm of phytophthora capsici to chilies and tomatoes and reduce the harm of pyricularia grisea to rice. The compound has the advantages of low use concentration, quick effect and broad spectrum, and provides a new way for improving the plant resistance, thereby having wide application prospects in agricultural production.

The invention relates to a botanical insecticidal composition containing 2-tridecanone and Saccharomyces alhagi and application of the botanical insecticidal composition. The botanical insecticidal composition is prepared from 2-tridecanone and Saccharomyces alhagi in a mass ratio of (1: 10)-(3: 1). According to the botanical insecticidal composition provided by the invention, a synergistic interaction effect can be achieved after the 2-tridecanone and the saccharomyces alhagi are mixed according to a specific mass ratio through a large number of experimental research screening, and the botanical insecticidal composition has a better insecticidal effect under the same dosage, so that the compounded composition has relatively high effective activity, and the application prospect is broad. The insecticidal composition can effectively prevent generation of pest resistance or delay the resistance speed, and is lower in cost and smaller in environmental influence. The botanical insecticidal composition provided by the invention has obvious prevention and treatment effects on multiple important lepidoptera pests such as armyworms, plutella xylostella, beet armyworms, cotton bollworms, cabbage caterpillars, prodenia litura and the like.

The invention relates to the field of petroleum drilling engineering, and discloses a plugging agent comprising an inorganic material with hydroxyl on the surface and a copolymer grafted on the surface of the inorganic material, a preparation method of the plugging agent and application of the plugging agent in plugging slurry for petroleum exploitation. The plugging agent is simple in preparation method and relatively low in cost, can be used for deep well and ultra-deep well operation, remarkably reduces the pressure transmission effect of well drilling, maintains relatively good well wall stability, effectively solves the problems of well wall instability, reservoir pollution and the like in well drilling work, improves economic benefits, reduces the occurrence rate of underground complex accidents, and has good application prospects. Guarantee is provided for the safety of drilling operation, and the application prospect is very wide.

The invention discloses a preparation method of a medical hydrophilic polyurethane sponge, and belongs to the technical field of polyurethane sponges, the preparation method comprises the following steps: stirring and mixing polyether polyol, polyester polyol, pure water, a foam stabilizer 9205, stannous octoate, a triethylene diamine propylene glycol solution with the mass fraction of 33% and a nano-silver antibacterial agent; the preparation method comprises the following steps: adding the modified ciprofloxacin into the polyurethane sponge, adding the polyisocyanate into the polyurethane sponge, stirring and mixing, adding the modified ciprofloxacin into the polyurethane sponge, stirring and mixing to obtain a mixed material, pouring the mixed material into a mold, standing, foaming, curing, and demolding to obtain the medical hydrophilic polyurethane sponge. According to the method for preparing the polyurethane sponge through the one-step foaming technology, the self-made nano-silver antibacterial agent and modified ciprofloxacin are introduced, the hydrophilicity and the antibacterial property of the polyurethane sponge are comprehensively improved, and it is detected that the hydrophilicity and the antibacterial property of the polyurethane sponge are better than those of the nano-silver antibacterial agent. The finally prepared medical hydrophilic polyurethane sponge has excellent hydrophilicity, antibacterial property and antibacterial stability.

This invention provides a method for preparing stem cells with enhanced osteogenic differentiation capacity, belonging to the field of biological technology. This invention significantly improves the osteogenic differentiation capacity of bone marrow mesenchymal stem cells (BMSCs) by combining deer antler polypeptide with rhodioloside. The osteogenic induction culture medium prepared in this invention includes DMEM / F12 medium, 10 mM β-glycerophosphate sodium, 50 μg / ml ascorbic acid, 100 nM dexamethasone, 20 μg / ml deer antler polypeptide, and 20 μg / ml rhodioloside. This optimized culture medium achieves a significant osteogenic promoting effect at lower concentrations, demonstrating the synergistic effect of deer antler polypeptide and rhodioloside. Simultaneously, the synergistic effect reduces the amount of components used, lowers the cost of using deer antler polypeptide, and reduces the side effects associated with high doses.

The invention discloses a silicon surface cleaning agent as well as a preparation method and application thereof. The silicon surface cleaning agent is prepared from the following raw materials in percentage by mass: 3 to 10 percent of composite acid, 0.1 to 0.5 percent of chelating agent, 2 to 3 percent of ethanol, 0.1 to 1 percent of DMSO (Dimethylsulfoxide), 1 to 3 percent of surfactant, 0.1 to 0.3 percent of metal cleaning aid and the balance of deionized water. The composite acid is composed of inorganic acid and organic acid according to the mass ratio of (2-5): (1-5). According to the invention, efficient and specific removal of potassium and sodium ions is realized, and the defect of the traditional cleaning process in the aspect is overcome; meanwhile, the cleaning agent also has an excellent cleaning effect on organic oil stains and other multiple metal ions, the process is simple and friendly, and the cost is low.

The application discloses application of 7-acetyl-lushanrubescensin A in preparation of anti-inflammatory cosmetics or medicines, and a composition containing the same and a preparation method, and belongs to the field of natural medicine research and development. The compound 7-acetyl-lushanrubescensin A is separated from isodon lichiangensis, can significantly inhibit release of NO and IL-6 inflammatory factors of RAW264.7 cells induced by LPS, and has better effect than a positive control dexamethasone in a concentration range of 1-3 mu g / mL, and has obvious anti-inflammatory activity. The 7-acetyl-lushanrubescensin A compound can be used as an anti-inflammatory active agent in skin inflammation care and / or inflammation repair cosmetics or medicines, and provides a new natural active ingredient source for care of inflammatory skin.

The present invention discloses an anionic surfactant, a foaming surfactant / foaming agent containing the anionic surfactant, and the preparation and application thereof. The gemini anionic surfactant is selected from at least one of the compounds represented by formula (I): R 1 Selected from C 7 ~C 41 Hydrocarbon or C 7 ~C 41 Substituted hydrocarbon group, repeated R 1 The same or different; a is any integer from 0 to 8; R 6 Selected from ‑COO ‑ ,‑SO 3 ‑ 、OSO 3 ‑ ,‑R 7 COO ‑ ,‑R 7 SO 3 ‑ or ‑R 7 OSO 3 ‑ , R 7 Selected from C 1 ~C 6 The alkylene group, repeated R 6 The system of the present invention contains multiple hydrophilic head groups with positive and negative charges, which can accurately control the electrostatic force between surfactant molecules, so that the foaming agent has both excellent foaming and stabilizing properties and very strong liquid carrying capacity.

The application discloses an organic annulus protection fluid for oil and gas wells and a preparation method thereof. The annulus protection fluid comprises 0.01-3wt% of active polymer, 0.1-5wt% of oxygen scavenger, 0.1-2.5wt% of bactericide, 0.1-0.5% of pH regulator, and the rest is water. The active polymer in the annulus protection fluid is copolymerized from N-acryloyl morpholine and acrylate compounds. The annulus protection fluid has good chemical stability, can effectively delay the corrosion rate of the casing and the oil and gas pipeline, solve the problems of fouling, bacterial reproduction, under-deposit corrosion and plugging caused thereby, and reduce the pressure difference between the oil and gas pipeline and the annulus. The system is suitable for annulus protection of oil and gas wells, and is especially suitable for high-acid and high-sulfur oil and gas wells.

The invention provides a device and a method for preparing a surfactant containing nano bubbles and electronic equipment, and belongs to the technical field of nano bubble preparation. The device comprises: a generation system comprising a reaction vessel for preparing the surfactant containing the nano-bubbles; the temperature control system is connected with the generation system and used for maintaining the temperature of the generation system; the gas storage system is connected with the generation system and is used for providing gas and pressure required by the generation system; the ultrasonic system is connected with the generating system and used for providing ultrasonic vibration for the generating system; and the control system is configured to store preparation steps and control actions of the temperature control system, the gas storage system and the ultrasonic system based on the preparation steps. According to the invention, the interfacial tension between the displacement system and crude oil can be greatly reduced only by using a small amount of surfactant, and when the displacement system with ultralow interfacial tension is used for oil reservoir development, the oil recovery efficiency can be improved and the development cost can be reduced.

The invention belongs to the field of biological medicines, and particularly relates to application of a phenolic acid compound and an antibiotic in preparation of a medicine for treating drug-resistant pathogenic bacterium infection. The phenolic acid compound provided by the invention is combined with antibiotics to completely kill multi-drug-resistant escherichia coli in an in-vitro experiment; under the same antibacterial effect, compared with the single use of the antibiotics, the use concentration of the antibiotics can be reduced by 8-32 times by the combined medication method; the compound can be used for improving the bioavailability of antibiotics and exerting a good effect in the treatment of in-vivo infection; the method of combined use of phenolic acid and antibiotics can also effectively reduce the problem of rapid evolution of bacterial drug resistance caused by independent use of antibiotics. The method is simple, efficient, stable, reliable and high in application value, and a brand new and feasible technical scheme is provided for effectively restraining clinical antibiotic multi-drug-resistant pathogenic bacteria.

The invention discloses an in-vitro dissolution method of a hydrobromic acid vortioxetine tablet, the in-vitro dissolution method adopts a paddle method, a dissolution medium is degassed purified water, and the dissolution medium contains 0.01% of cetyltrimethylammonium bromide (CTAB). The method comprises the following steps: taking samples at fixed time points at intervals, detecting the concentrations of the votioxetine hydrobromide samples at each time point by using a high performance liquid chromatograph to obtain a votioxetine dissolution curve which is good in homogeneity, smooth and free of jump points (that is, the dissolution amount at the latter time point is lower than that at the former time point), and predicting the in-vitro similarity of a tested preparation and a reference preparation. The cetyltrimethyl ammonium bromide (CTAB) is selected as a surfactant, compared with conventionally used lauryl sodium sulfate (SDS) and Tween 80, the cetyltrimethyl ammonium bromide has a better dissolution effect at a lower concentration, the dosage is smaller, the data uniformity is good, the relative standard deviation (RSD) is within 2%, and the accuracy and reliability of dissolution detection are remarkably improved. The dissolution method adopted by the invention can simulate the in-vivo release and absorption process, and the f < 2 > factor is combined to analyze the preparation difference, so that the quality and process difference between the reference preparation and the tested preparation can be effectively distinguished, and the evaluation on the dissolution behavior and quality consistency between the hydrobromic acid vortioxetine tablets is realized.