168 results about "Articular cavity" patented technology

The invention provides a method for preparing injectable microgelparticles, and the method comprises the following steps: adopting hyaluronic acid dry powder in accordance with standard requirements, carrying out cross-linking reaction to form water-insoluble cross-linking hyaluronic acid gel, carrying out postprocessing of dynamic dialysis, rotary crushing and centrifugal separation to form injectable microgelparticles. The method is safe and effective, and simple in processing flow, and can be used for adjusting the particle size of the microgelparticles by virtue of control on process parameters. The method has the advantages of good biocompatibility, internal anti-degradation capability, and suitability for subcutaneous filling, substituation of articular cavity lubricating fluid, conglutination prevention of internal wound and the like.

The invention relates to a biological material for repairing meniscus tear and a preparation method for the biological material. The material consists of an acellular matrix membrane on the inner layer, a porous structure collagen matrix membrane on the middle layer, a cell layer formed by cartilage cells on the outer layer and a cell sheet. The repair material has certain elasticity and toughness and is high in biological activity; the cartilage cells can secrete cell factors per se, and nutritional ingredients can be directly obtained through articular cavity synovia, and thus, the establishment of communication link between tissue cells at both ends of the tear during the meniscus tear process is accelerated; the immigration and the infusion of the cells in the material and autologous tissue cells are promoted; the tissue regeneration and functional reconstruction time is shortened; and the ideal novel material and the preparation method for the material are provided for clinically repairing the damage of the meniscus tear (including blood supply zones), especially the tissue regeneration and the functional reconstruction of no-blood zones and low-blood zones.

The invention belongs to the technical field of medical polymer materials and in particular relates to X ray developing thermotropic hydrogel and a preparation method thereof. The X ray developing thermotropic hydrogel comprises an iodine-containing amphiphilic block copolymer and a solvent, wherein the iodine-containing amphiphilic block copolymer is obtained by bonding an iodine-containing micromolecule and an amphiphilic block copolymer by virtue of a covalent bond, and thermotropic gelatinization phase transformation can be carried out on a water system of the X ray developing thermotropic hydrogel along with temperature increase. The thermotropic hydrogel can be implanted under the skin and implanted into an abdominal cavity, an articular cavity and other specific parts of a human body in a mode of injection, and in situ formed hydrogel has good X ray developing performance, clear positioning and long-term tracing observation can be carried out on the hydrogel by adopting an X ray radiography technique. The thermotropic hydrogel also can serve as a medicine controlled release carrier, a tissue repairing support, a blood vascular embolization agent, a tissue marker and the like and can be used for realizing integration of diagnosis and treatment.

The invention relates to a temperature-sensitive in-situ gel preparation composition for intra-articular injection and a preparation method thereof, and belongs to the field of medicine preparations. The preparation method comprises the following steps: preparing diclofenac sodium-carrying sodium alginate microspheres by combining a microsphere technology with a temperature-sensitive gel technology, and then carrying the medicine-carrying microspheres in a chitosan/beta-sodium glycerophosphate temperature-sensitive in-situ gel system to obtain a diclofenac sodium in-situ gel preparation composition. The composition is in a liquid state at a room temperature, capable of being converted to a semi-solid gel at a body temperature and forming a medicine storeroom at a medication part after being partially injected to enter an articular cavity, and beneficial to slowly releasing the medicine, prolonging the time of staying at the injection part, of the medicine, and enhancing the efficacy. The composition disclosed by the invention can be used for treating arthritis diseases of rheumatism, rheumatoid arthritis, osteoarthritis, synovitis and the like, as well as applicable to both diclofenac sodium and other arthritis treatment medicines of non-steroidal anti-inflammatory medicines, steroid hormone medicines, biological preparations and the like.

The present invention provides an intra-articular injection administration preparation containing trehalose, which is characterized in that the preparation contains the trehalose. The present invention can improve the capabilities of the cartilage cells to adapt to and resistant to the environmental changes through the intra-articular injection administration, at the same time, the trehalose has the significant effects of antioxidant and anti-free radicals, which can alleviate the symptoms of arthritis; in addition, the trehalose also provides the necessary sugar for cartilage repair, so as to repair and reconstruct the cartilage. The present invention is used for improving and treating the rheumatism, rheumatoid arthritis, osteoarthritis, synovitis and/or cartilage injury.

The present invention provides a pharmaceutical composition used for intra-articular injection, the main ingredients of which are sodium hyaluronate and chondroitin sulfate, wherein, the molecular weight of the sodium hyaluronate is 1.2 million to 5 million Daltons and the concentration is 0.5 percent to 2.0 percent, and the molecular weight of the chondroitin sulfate is 2000 to 15000 Daltons and the concentration is 1 percent to 5 percent. The present invention has the advantages of good viscoelasticity and good buffer capacity of the sodium hyaluronate with relatively high molecular weight, as well as the advantages of rapid absorption and rapid repair of the chondroitin sulfate with relatively low molecular weight, thus the invention can be used for treating the arthritis by intra-articular injection.

The invention mainly provides thermo-sensitive in-situ gel for articular cavity injection and a preparation method thereof. The gel is prepared from a drug-loaded biodegradable high-molecular polymer in a physical crosslinking way. The high-molecular polymer is prepared by connecting hyaluronic acid and a temperature-sensitive material through chemical bonds; when the temperature is lower than the body temperature, the drug can be loaded or dispersed into the polymer to form a nanogel suspension; and the temperature of an articular cavity injection part is raised to the body temperature, the preparation is converted from the nano suspension to the in-situ gel. The gel provided by the invention is free of chemical crosslinking, high in safety and capable of taking the synergic treatment effects of a nonsteroidal anti-inflammatory drug and the hyaluronic acid, reducing the toxic and side effects and improving the compliance of a patient.

An articular cavity prosthesis comprises a block extending along a central axis and including a top section and a base section, the top section having an articular bearing surface extending transverse to the central axis and a supporting surface opposite the articular bearing surface configured to contact a surface of a bone on which the block is to be mounted, the base section having a fixing protrusion protruding outward from the supporting surface along the central axis and having a volume V, the block being formed of human tissue.

The invention discloses a mesenchymal stem cell preparation for repairing articular cartilage injury or coloboma, a preparation method and application thereof. According to the preparation method, mesenchymal stem cells and synovial cells are indirectly cocultured, so as to obtain the mesenchymal stem cells after coculture, and then the mesenchymal stem cells are cryopreserved, umbilical cord mesenchymal stem cells, which are acquired after the synovial cells and the umbilical cord mesenchymal stem cells are inoculated at density ratio of 4:1 and cocultured, are easier to differentiate towardcartilage direction under a same induction condition. The preparation method utilizes a constructed animal knee joint cartilage injury or coloboma model to prove that the umbilical cord mesenchymal stem cells cocultured with the synovial cells have an obvious effect of facilitating the repairing of articular cartilage injury or coloboma. The invention further provides a preparation which is prepared from the cocultured mesenchymal stem cells. The preparation can be applied to clinic repairing of articular cartilage injury or coloboma; the preparation can effectively promote the repairing of articular cartilage injury or coloboma through anticular injection; the therapeutic effect of the preparation is obviously better than that of a pure mesenchymal stem cell preparation.

The invention discloses a non-viral nanometer nucleic acid transportation compound used for treating cartilage defect through intra-articular injection which comprises low-molecular chitosan with molecular weight of 5KD-100KDand percentage composition of amino group of 30-70%, and nucleic acid, wherein N/P of the compound is 0.2:1-10:1.The invention degrades high molecular chitosan to low molecular chitosan, increases water solubility of material, reduces viscosity of solution, reduces influence of sour environment of solvent to cell, improves compound size with nanometer, is in favor of performing genetic transmission to cartilago articularis cell, and protects target nucleic acid from neutralizing with synovial fluid and being degrade by nuclease in lysosome. The invention has simple preparation and abroad application scope; can solve engineering roadblock of cartilage repair with no requirement of operation, complex device and bracket material.

The invention relates to application of a phellinus igniarius aqueous extract in trioxypurine resistance. The phellinus igniarius aqueous extract mainly comprises two substances with mass-to-charge ratio of 1015.0270 and 1029.2048 daltons; the spectral strength of the two substances in mass spectrometric detection is 60% and 100%. A phellinus igniarius alcohol is prepared according to the following method: crushing phellinus igniarius growing in Changbai Mountain region into powder; adding water to the phellinus igniarius powder, and uniformly stirring; boiling for 2h in a boiling water bath to obtain a mixed solution; cooling the mixed solution; centrifuging at superspeed; collecting supernate; filtering the supernate to obtain the phellinus igniarius aqueous extract; sterilizing under the temperature of 121 DEG C and high pressure to obtain the phellinus igniarius aqueous extract. Compared with colchicine, the phellinus igniarius aqueous extract has the effect of reducing depositingtrioxypurine in articular cavity, so that the trioxypurine content in a human body can be reduced through the phellinus igniarius aqueous extract, to treat gout.

The invention discloses a high-strength gradient full-absorption artificial ligament and a preparation method of the high-strength gradient full-absorption artificial ligament. The artificial ligamentcomprises an articular cavity free section and marrow channel woven sections at the two ends of the articular cavity free section, wherein the marrow channel woven sections are of sheath-core double-layer tube-shaped structures, and the sheath-core double-layer tube-shaped structures are composed of sheath layers and core layers. The preparation method comprises the following step: preparing themarrow channel woven sections of the sheath-core double-layer tube-shaped structures by adopting a weaving, knitting or braiding integrated forming technology, wherein weft yarns are not arranged at the articular cavity free section, namely, parallel free yarns not participating in weaving are adopted. The adopted yarns are formed by twisting and compounding fibers with different degrading periodsat the specific ratio, after implantation, a three-dimensional spiral through porous channel can be constructed through gradient degrading, thus the oriented growth of host cells is induced, and meanwhile, the requirement for the mechanical property needed during the tendon-bone healing process is met.

The invention relates to a drug composite, which is characterized in that the drug composite comprises the following components: a) one of isoquinoline composites and b) one or the mixture of two of hyaluronate or hyaluronic acid derivative. The composite of the invention can be used for preparing drug preparations which are taken in a non-oral may, is especially used for preparing the drug preparations for treating relevant diseases in the partial administration of articular cavity and posterior segment eye and has the characteristics of good treatment effect and less toxic and side effects.

The present invention relates to one kind of compound triptolide alcohol preparation for being injected into articular cavity to treat osteoarthritis. It includes triptolide alcohol solution of 1*10<-4> to 5*10<-2> mg/ml concentration and gel liquid mixed in the volume ratio of 1 to 1-9. It has medicine slow releasing effect, and this can prolong the action period of medicine, reduce consumption of triptolide alcohol and lower the toxic side effect of the medicine. It can inhibit the further development of osteoarthritis in early and middle phase, improve the environment inside the joint and promote the regeneration and repair of articular cartilage to reach the aim of cure osteoarthritis.

The invention discloses a cell gel preparation for treating articular cartilage injury and a use thereof, and a used gel solution for maintaining the activity of cryopreserved cells. The formula of the cell gel preparation comprises, according to the volume percentage, 50% to 95%, of the gel solution and 5% to 50% of a compound electrolyte cell resuspension, and per milliliter of the cell gel preparation contains 0.5*10<5>-2*10<7> cells. The gel solution includes the following components by mass and volume: 1 to 50 mg of a high molecular material stabilizer, 0.1 to 1 mg of chondroitin sulfate,5 to 50 mg of human serum albumin, 30 to 150 [mu]l of glycerol, 1 to 30 [mu]l of DMSO, and 700 to 900 [mu]l of a compound electrolyte injection liquid or amino acid injection liquid or normal salineinjection liquid. The gel preparation has good biocompatibility and can be directly stored at low temperature for a long time and maintain high cell viability, and does not require traditional liquidnitrogen cryopreservation; the cell gel preparation after resuscitation can maintain cell biological characteristics and functions and high survival rate in a joint cavity, and can be used in treatment of articular cartilage injury.

The invention belongs to the technical field of medical sciences, and discloses a knee osteoarthritis course detection method based on near-infrared light. The knee osteoarthritis course detection method comprises the steps of processing clinic knee joint CT pictures by using an image segmentation processing technology, and remaining muscle and skeleton tissue portions and performing gray scale contrast ratio reinforcement and edge extraction; and then respectively simulating movement curves of near-infrared photons in knee joints of patients having early, medial and later stages of arthritis through analyzing optical characteristic parameters of articular cavity synovia by using the Monte Carlo method based on the processed CT pictures, fitting infrared photon emitting distribution characteristics of different courses by using the Gaussian function, and determining patient conditions by using the effective photon emitting rate and symmetric axis coordinates of the fitting function as double indexes. Experimental results show that accuracy of the method reaches more than 92%, the near-infrared detection is a nondestructive testing means, and the knee osteoarthritis course detection method has large application value in knee osteoarthritis clinic detection application.

The invention relates to a preparation method of an osteoarthritis animal model. The method comprises the following steps: blending a matrix metalloproteinase-3 with normal saline so as to obtain a 5-10nmol/L matrix metalloproteinase-3 solution; blending a urokinase-type plasminogen activator with the normal saline so as to obtain a 2-4nmol/L urokinase-type plasminogen activator solution; then, blending the two solutions in proportion of 3:1 or 2:1 so as to obtain a mixed solution; injecting 0.5mL/kg of mixed solution into an articular cavity of an experimental animal. According to the method, the matrix metalloproteinase-3 and the urokinase-type plasminogen activator which are of conventional chemical reagents in a laboratory are taken as raw materials; the mixture is injected into the articular cavity of the animal, so that the rapid degeneration of cartilage tissues is generated. Thus, the pathogenesis process of osteoarthritis is simulated, thereby bringing great convenience for the research of an osteoarthritis intervention treatment technology. The method has the characteristics of being simple and convenient to operate, rapid in modelling, economical, practical, convenient in material obtainment, and the like.

The invention discloses a hyaluronic acid derivative and a preparation method thereof, wherein the hyaluronic acid derivative is obtained via covalent combination of a phenolic hydroxyl group-containing isoquinoline compound with hyaluronic acid via intracorporal degradable molecular bridges. The hyaluronic acid derivative can be used for preparing medicines used for nonoral administration, and especially used for preparing slow-release medicines which are used for treating related diseases via articular cavity and eye posterior segment topical administration; and is excellent in curative effect; and toxic and side effect is less.

The invention relates to a special-shaped bone fracture plate from a pelvis arcuate line to a tuberculum pubicum. The special-shaped bone fracture plate comprises a torsional arc-shaped section and a bended section on the rear side at the rear end, wherein the arc-shaped section of the bone fracture plate corresponds to the pelvis arcuate line and a pecten pubis, the torsional section on the rear side at the rear end of the bone fracture plate corresponds to the inner face of the tuberculum pubicum, and the arc-shaped section of the bone fracture plate is integrally connected with the bended section on the rear side at the rear end to form the reconstructed bone fracture plate which changes three-dimensionally. Two sections of planes of the bone fracture plate are intersected at angles to form a beam-shaped structure. The bone fracture plate has the advantages that the bone fracture plate is good in adaptability, the surgical difficulty and degree of operative wounds are reduced, a fixed bolt is prevented from being implanted into an articular cavity and the like.

The invention belongs to the medical field and particularly relates to a contrast media injection being suitable for MR arthrography. The injection is prepared from the following raw materials: 0.05-0.25 ml of gadopentetate dimeglumine, 1.25-5 ml of iohexol, 1.25-5 ml of lidocaine and 0-10 ml of physiological saline. The injection has following advantages: 1) under guidance of X ray or CT, the injection, when being injected into articular cavity for performing the MR inspection to the arthrosis that needs to inspect, can be used for development under both X-ray and MR inspection, wherein according to different inspection positions, only does corresponding proportion adjustment need to carry out, thus greatly simplifying operation procedure of medical staffs; 2) the injection has low permeability, low toxic and side effects, high bio-safety, low toxicity on nervous system, low generation rate of side reactions, and good body tolerance; and 3) the injection can generate high-intensive magnetic resonance signal and increase signal intensity of magnetic resonance imaging, so that an image can be displayed clearly.