15 results about "Hyaline cartilage" patented technology

The present invention relates to a method for using a lyophilization hyaline cartilage powder to produce a composition for regenerating cartilage, and a composition for regenerating cartilage produced by using the method, the method comprising: A) a step for preparing hyaline cartilage; B) a step for freeze-drying and crushing the hyaline cartilage, and producing a lyophilization hyaline cartilage powder; C) a step for producing an adipose tissue extract from autologous adipose tissue; and D) a step for producing a composition which is for regenerating cartilage and including the lyophilization hyaline cartilage powder and the adipose tissue extract.

A method for treating cartilage defects including providing a placental membrane preparation that comprises ground or minced placental membranes and optionally, a ground or minced cartilage and / or biocompatible glue, and introducing the preparation to a cartilage defect within a skeletal joint. The cartilage defect may comprise a hyaline cartilage defect, such as a chondral defect, or meniscal defect. The treatment may be provided in combination with other treatments such as marrow stimulation treatments and surgical repair treatments using sutures or other fixation techniques. The preparation promotes the regeneration of cartilage within the skeletal joint.

The invention relates to a functionalized cartilage tissue repair material as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering. The invention aims to solve the problems that the existing decalcified bone matrix is insufficient in cartilage induction in cartilage repair, and is easy to cause blood vessel ingrowth and fibrosis repair. The preparation method comprises the following steps: inoculating cartilage cells on a three-dimensional porous frame structure carrier, carrying out in-vitro dynamic pre-culture to enable the cartilage cells to secrete an extracellular matrix, and carrying out decellularization treatment to obtain the functionalized cartilage tissue repair material retaining the cartilage specific matrix. The material has a suitable pore structure and abundant II-type collagen deposition, can effectively promote chondrocyte differentiation and hyaline cartilage regeneration, inhibits blood vessel ingrowth, maintains a cartilage vascular-free microenvironment, significantly improves the structure and function reconstruction effect of cartilage defect repair, and has good biocompatibility and clinical transformation potential.

The application discloses a customizable biphasic hydrogel scaffold, which comprises a transparent cartilage layer and a subchondral bone layer, the transparent cartilage layer comprises methacrylated type I recombinant collagen and bone marrow mesenchymal stem cells, and the subchondral bone layer comprises methacrylated type I recombinant collagen and resveratrol-loaded bioactive glass. The application further provides a preparation method and application of the biphasic hydrogel scaffold. The biphasic hydrogel scaffold has the advantages of customization, antioxidation, anti-inflammation, high biocompatibility, promotion of wound healing and bone repair and the like.

The present invention provides a technique for providing a cell culture suitable for cartilage repair, particularly a cell culture suitable for hyaline cartilage repair.　Provided is a marker for evaluating chondroid tissue formation characteristics that includes one or more gene markers selected from the group consisting of (1) adhesion-related genes, (2) angiogenesis-related genes, (3) ECM-related genes, (4) collagen-related genes, (5) cell migration-related genes, and (6) mitochondrial-related genes.

The application discloses a hydrogel for promoting cartilage repair by piezoelectric effect, a preparation method and application thereof, wherein the hydrogel body comprises biodegradable three-dimensional piezoelectric hydrogel prepared from sodium potassium niobate, polyvinyl alcohol and polyacrylic acid. The polyvinyl alcohol is a gel state with a porous structure, the polyacrylic acid is a polyelectrolyte with a negative charge, and the sodium potassium niobate is a piezoelectric material. The piezoelectric hydrogel with the non-toxic and harmless sodium potassium niobate nanoparticles with excellent piezoelectric performance and the polyvinyl alcohol and polyacrylic acid as the main body develops a biodegradable three-dimensional piezoelectric support. The support is implanted into a knee joint cartilage defect, when the knee joint moves, the three-dimensional piezoelectric support generates an electric charge due to stress extrusion and joint self-friction, the composite piezoelectric hydrogel can generate an electric signal in response to radio stimulation to promote chondrogenic differentiation of stem cells and endogenous angiogenesis, perfect regeneration of hyaline cartilage at the cartilage defect and acceleration of recovery of the motion function.

This invention relates to a novel method for producing cartilage tissue in vitro, as well as methods for therapeutic uses and screening of the cartilage tissue produced therefrom.

To provide pharmaceutical compositions for osteoarthritis treatment, comprising immune-tolerized fetal stem cell-derived extracellular vesicles containing various components that promote hyaline cartilage regeneration and relieve inflammation at higher levels than those stem cell-derived extracellular vesicles obtained under general culture conditions, and thus exhibiting superior hyaline cartilage regeneration and anti-inflammatory efficacy as well as methods for manufacturing the extracellular vesicles and compositions for osteoarthritis treatment using the extracellular vesicles.SOLUTION: These extracellular vesicles derived from immune-tolerized fetal stem cells contain fibroblast growth factor-2 (FGF-2), fibroblast growth factor-7 (FGF-7), insulin-like growth factor-1 (IGF-1), transforming growth factor-beta 1 (TGF-β1), bone morphogenetic protein-4 (BMP-4), bone morphogenetic protein-5 (BMP-5), and bone morphogenetic protein-7 (BMP-7) and HLA-G protein.SELECTED DRAWING: Figure 1

The invention relates to the technical field of biological medicine, in particular to a traditional Chinese medicine composition for promoting regeneration of hyaline cartilage and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-30 parts of prepared rehmannia root, 10-30 parts of astragalus membranaceus, 10-20 parts of Chinese yam, 10-20 parts of cistanche deserticola, 5-15 parts of angelica sinensis, 5-15 parts of corydalis tuber, 5-15 parts of spina date seed and 3-10 parts of safflower carthamus. The traditional Chinese medicine composition has the traditional effects of tonifying kidney and replenishing essence, tonifying qi and activating blood, and activating meridians to stop pain, and is proved to be capable of effectively relieving oxidative stress of a local microenvironment of joint injury and remodeling metabolic homeostasis of mesenchymal stem cells by up-regulating expression of HIF-1alpha and PLK1, so that regeneration of high-quality transparent cartilage is specifically induced, formation of fibrocartilage is inhibited, and the joint injury effect is improved. The composition is used for preventing or treating articular cartilage defects and osteoarthritis.

The invention discloses a customizable biphasic hydrogel scaffold which comprises a hyaline cartilage layer and a subchondral bone layer, the hyaline cartilage layer comprises methacrylated I-type recombinant collagen and bone marrow mesenchymal stem cells, and the subchondral bone layer comprises methacrylated I-type recombinant collagen and bioactive glass loaded with resveratrol. The invention also provides a preparation method and application of the double-phase hydrogel scaffold. The biphase hydrogel stent has the advantages of being customizable, resistant to oxidation and inflammation, high in biocompatibility, capable of promoting wound healing and bone repair and the like.

The invention discloses a human mesenchymal stem cell preparation activated by specific immune pretreatment and application thereof, and the application is characterized in that human mesenchymal stem cells (hAMSCs) and autologous human peripheral blood lymphocytes are co-cultured and pretreated according to a specific ratio (such as 100: 1), and then are applied to a knee osteoarthritis model through intra-articular injection. The synergistic treatment effect which is obviously superior to that of the unpretreated hAMSCs can be generated; the Co-hAMSCs show a unique in-vivo action mode, the survival time of the Co-hAMSCs at the local part of the joint is shorter than that of common hAMSCs, but the Co-hAMSCs can quickly relieve pain in the early treatment stage, improve the joint function and lower local inflammation; the cartilage repair and regeneration can be more effectively promoted for a long time, and the specific expression is as follows: the cartilage histological score is obviously improved, the content of cartilage matrix glycosaminoglycans (especially middle and deep layers) is increased, and the repaired tissue is guided to be transformed to a hyaline cartilage sample phenotype with high expression of type II collagen and low expression of type I collagen, so that the cartilage repair and regeneration can be more effectively promoted. And inflammatory factor balance of local joints and the whole body can be synchronously adjusted.

The present invention belongs to the field of tissue engineering and regenerative medicine, and specifically relates to a 3D cartilage tissue, a preparation method thereof, and an application thereof. In response to the problems in the prior art such as the limited source of mesenchymal stem cells and the easy fibrosis of induced cartilage, the present invention reprograms skin fibroblasts into cartilage precursor cells through transcription factor transfection combined with multi-stage small molecule regulation, and utilizes a dynamic 3D culture system to optimize the formation and functional maintenance of cartilage particles. Experiments have confirmed that the expression of cartilage tissue marker genes induced by this method is normal, COL10 is negative, Alcian blue staining and immunohistochemistry verify its hyaline cartilage characteristics, and in vivo repair experiments show significant therapeutic effects. The raw materials of this solution are easily available and the cost is controllable, providing an efficient and stable technical platform for cartilage defect repair and organoid research.

The invention provides a 3D printing knee joint cartilage repair scaffold integrated with a drug sustained release function, and belongs to the technical field of tissue engineering and intelligent biological materials. The stent is personalized and customized based on image data of a patient, and has gradient pore structures of a cartilage phase, a transition phase and a bone phase, a spiral interpenetrating nutrition channel, a partitioned microfluidic drug chamber and a mechanical response micro-column array. Through the pH / enzyme double-response plugging membrane, time-space accurate release of anti-inflammatory drugs, growth factors and antibiotics is realized, and pulse-type drug delivery is triggered under a joint activity load. The stent significantly promotes regeneration of transparent cartilage. The problems that an existing stent is single in structure, uncontrollable in drug release, insufficient in deep nutrition and disjunction in mechanics and biology are solved, the stent has excellent biocompatibility, mechanical stability and clinical convertibility, and an efficient and intelligent integrated regeneration solution is provided for knee joint cartilage injuries.

The invention belongs to the technical field of biomedical materials, and particularly relates to a highly-bionic gradient artificial cartilage scaffold material, a preparation method and application, the highly-bionic gradient artificial cartilage scaffold material comprises a hyaline cartilage layer, a calcified cartilage layer and a subchondral bone layer, the calcified cartilage layer is composed of mineralized I-type collagen fibers containing 30% of nano-hydroxyapatite, and the subchondral bone layer is composed of mineralized I-type collagen fibers containing 60% of nano-hydroxyapatite; the prepared scaffold highly simulates natural osteochondral tissues, has similar composition, nano structure and mechanical properties with the natural osteochondral tissues, and presents an obvious gradient; the hydrogel has excellent biocompatibility and biological activity, and cell proliferation and gradient differentiation of bone marrow mesenchymal stem cells can be remarkably promoted; in a rabbit osteochondral defect model, osteochondral tissue regeneration is remarkably promoted, and the compound can be applied to clinical treatment of osteoarthritis.

The present invention relates to a method for using a lyophilization hyaline cartilage powder to produce a composition for regenerating cartilage, and a composition for regenerating cartilage produced by using the method, the method comprising: A) a step for preparing hyaline cartilage; B) a step for freeze-drying and crushing the hyaline cartilage, and producing a lyophilization hyaline cartilage powder; C) a step for producing an adipose tissue extract from autologous adipose tissue; and D) a step for producing a composition which is for regenerating cartilage and including the lyophilization hyaline cartilage powder and the adipose tissue extract.