92 results about "Type II collagen" patented technology

The invention belongs to the technical field of microbial fermentation, and particularly relates to lactobacillus rhamnosus capable of maintaining bone health and improving local joint inflammation and a metagen and application of the lactobacillus rhamnosus. According to the invention, the probiotic NKU ML1-2 is subjected to fermentation culture, such that a post-generation product is prepared. Results of embodiments prove that the metagen product can enhance the oxidation resistance of cartilage and osteoblasts and promote the synthesis of anti-inflammatory factors and cartilage matrixes (type II collagen and proteoglycan), and has the potential of relieving oxidative stress and inflammatory response of local joints, relieving joint inflammation and delaying cartilage destruction. The prepared metagen product is high in safety and good in stability, and can be applied to preparation of medicines for preventing and / or treating osteoarthritis; the metagen product can also be used for promoting bone health, enhancing the antioxidant capacity of osteoblasts and / or chondrocytes, promoting osteoblast activity and / or promoting calcium absorption and fixation, and a new solution is provided for joint health management.

The invention provides a preparation method of non-denatured type II collagen capable of increasing bone mineral density and improving bone elasticity, and belongs to the technical field of biologication.The preparation method comprises the steps that an EDTA-citric acid buffer solution containing beta-mercaptoethanol is adopted for decalcifying cartilage, a supercritical CO2 extraction technology is adopted for degreasing, and the collagen is obtained; alkaline protease and collagenase II are adopted for substrate directional enzymolysis and gradient digestion; then the non-denatured type II collagen is separated by adopting three-phase centrifugal separation, and the non-denatured type II collagen with high extraction rate, high purity and high structural integrity can be obtained by adopting bionic H column chromatography purification.

This invention provides a composition for improving bone and joint health, its preparation method, and its application, relating to the field of bone and joint health products technology. By weight, the composition for improving bone and joint health comprises the following components: 0.5-5 parts collagen peptides, 5-30 parts mangosteen extract, 0.5-3 parts non-denatured type II collagen peptides, 0.1-2 parts milk mineral salts, 0.3-3 parts bone collagen peptides, 0.1-2 parts cartilage extract, 0.1-2 parts N-acetylglucosamine, 0.1-2 parts sodium hyaluronate, and 0.3-3 parts turmeric extract. This invention, through multiple combinations of mangosteen extract and turmeric extract, and cartilage extract and sodium hyaluronate, obtains a composition that is low in cost, highly safe, and has a good effect on the prevention or improvement of bone and joint diseases.

The invention relates to a diatomic iron-iron site nano-enzyme constructed by relieving oxidative stress and cartilage degeneration and used for targeted osteoarthritis treatment. According to the invention, a nitrogen-doped porous carbon anchored diatomic iron nano enzyme catalyst (Fe2-NCs) with Fe-Fe dimer coordination is developed by using a'subject-object 'strategy. The Fe2-NCs protect cartilage cells from oxidative stress induced apoptosis by modulating ROS and active nitrogen species (RNS) and promoting O2 release. In addition, Fe2-NCs restores mitochondrial function by inhibiting NOX4 expression, improving ATP production, and normalizing COXIV levels. In an in-vivo OA model, the Fe2-NCs can reduce the expression of a pro-inflammatory medium COX-2 through an NF-kappa B signal channel, inhibit the up-regulation of MMP-13 and delay the degradation of type II collagen. The invention provides a new theoretical framework and methodological approach for the treatment of osteoarthritis, and has important clinical and scientific significance.

The invention provides a pharmaceutical composition for bone joints as well as a preparation method and application of the pharmaceutical composition. The pharmaceutical composition is prepared from the following components in parts by weight: 20 to 2000 parts of N-acetylglucosamine and 10 to 1000 parts of non-denatured type II collagen. In the compound composition of the N-acetylglucosamine and the non-denatured type II collagen, the two core components play a complementary role: the N-acetylglucosamine is focused on promoting synthesis of a cartilage matrix and provides a material basis for cartilage repair; the non-denatured type II collagen reduces damage of autoimmune response to cartilage through immunoregulation, the non-denatured type II collagen and the non-denatured type II collagen have a synergistic effect, the symptoms such as arthralgia and swelling can be relieved, the joint structure can be improved from the double angles of repairing and protecting, and particularly the treatment effect on patients with severe osteoarthritis is remarkably superior to that of a single-component preparation; and an innovative and efficient choice is provided for the treatment of osteoarticular diseases.

The application discloses a functional composition for improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component; the first active component comprises elastin peptide, haematococcus pluvialis and ergothioneine; and the second active component comprises one or more of houttuynia cordata and non-denatured type II collagen. The application realizes the protection effect on joint, cartilage and bone health by scientific matching of the components and synergistically improving the glycosaminoglycan level in chondrocytes and the osteocalcin level in osteoblasts. Meanwhile, the components in the functional composition for improving bone and joint health are natural and have good biocompatibility, and have no obvious side effects, so that the application provides a kind of drug or a substitute for the treatment and prevention of bone diseases.

The application provides a preparation method of non-denatured type II collagen for increasing bone density and improving bone elasticity, and belongs to the technical field of biotechnology. The application adopts EDTA-citric acid buffer containing beta-mercaptoethanol to decalcify cartilage, adopts supercritical CO2 extraction technology to defat, adopts alkaline protease and collagenase II to perform matrix directional enzymolysis, and performs gradient digestion; then three-phase centrifugal separation is adopted to separate non-denatured type II collagen, and biomimetic H column chromatography purification is adopted, so that non-denatured type II collagen with high extraction rate, high purity and high structural integrity can be obtained.

A method (10) of forming a gel suspension of an active ingredient such as undenatured type II collagen and / or a microbiome modulator. The method (10) comprising the steps of combining a natural flavouring and / or preserve and water, and heating in a first heating stage. The method (10) further comprises adding a thickening agent, e.g. xanthan gum. The method (10) further comprises adding a hydrated pectin mixture, and heating in a second heating stage. The second temperature being higher than the first temperature, preferably not exceeding 85 degrees Celsius. The method (10) further comprises adding the active ingredient(s) and rapid cooling to form a suspension.

The invention relates to the technical field of biological detection, in particular to an SERS (Surface Enhanced Raman Scattering) detection method of an osteoarthritis marker type II collagen. The SERS detection method of the osteoarthritis marker type II collagen comprises the following five steps: synthesizing gold nanoparticles, functionalizing an SERS substrate, coupling an SERS probe, establishing a detection standard curve and carrying out SERS spectrum detection. According to the method, the SERS signal of the osteoarthritis marker type II collagen is specifically enhanced through the plasmon resonance effect of the functionalized gold nanoparticles, trace detection of the type II collagen is achieved, detection can be achieved only by simply diluting or centrifuging a sample, purification and concentration steps needed by ELISA detection are avoided, a result is obtained within a short time, and the method is suitable for large-scale popularization and application. The kit is suitable for convenient and rapid diagnosis of early osteoarthritis; meanwhile, the invention provides an SERS (Surface Enhanced Raman Scattering) detection kit for the osteoarthritis marker type II collagen, which can be synthesized and stored on a large scale, so that the detection time is comprehensively shortened, and the detection efficiency is improved.

This invention relates to a compound nutritional composition and its application. The compound nutritional composition comprises the following components: 1 part by weight of turmeric; 0.002-5 parts by weight of enzymatically hydrolyzed bone powder; and 0.02-0.2 parts by weight of cartilage powder containing type II collagen or 0.02-10 parts by weight of collagen peptide powder. The compound nutritional composition provided in this application addresses the specific osteoarthritis issues of athletes. By constructing an articular cartilage cell inflammation model and conducting population testing, the intervention effects of the compound nutritional composition provided in this application on exercise-related oxidative stress, inflammatory response, and pain perception are systematically evaluated. The aim is to overcome the limitations of traditional broad-based formulations and develop precise nutritional solutions that combine component synergy with exercise physiological adaptability, providing athletes with more scientific and effective joint health protection.

This invention relates to the field of biomedical materials, providing non-denatured type II collagen ultrafine powder with cartilage tissue-targeted growth and structural repair functions, its bioactivity preservation preparation method, and its application in joints. The ultrafine powder has an average particle size of 0.5-50 μm, a triple helix structure integrity rate of not less than 85%, a thermal denaturation temperature of 38-48℃, and retains chondrocyte binding domains CB10, CB11, and MMP cleavage sites. The preparation method includes raw material pretreatment, time-limited enzymatic hydrolysis, graded purification, and ultra-low temperature ball milling. The product can be used for the repair and treatment of cartilage degenerative diseases such as osteoarthritis and cartilage damage.

The invention provides a glucosamine and chondroitin sulfate pharmaceutical composition as well as a preparation method and application thereof, and belongs to the technical field of biological pharmaceutical preparations, the composition comprises sodium chondroitin sulfate, glucosamine sulfate, dimethyl sulfone, non-denatured type II collagen, frankincense extract and auxiliary materials in specific parts by mass, compared with the prior art, the composition provided by the invention has the advantages that by selecting the components, adding the composite synergist optionally and optimizing the ratio and synergistic effect of the components, the effects of the composition in promoting cartilage regeneration and inhibiting joint inflammation are remarkably improved, the anti-inflammatory and anti-oxidation effects are enhanced, and the bioavailability is also improved, so that a breakthrough progress is realized in the field of joint health.

The application provides a composition containing curcuma and type II collagen-containing cartilage powder and application thereof in joint maintenance, and relates to the field of food technology. The application provides a composition containing milk mineral salt, curcuma, type II collagen-containing cartilage powder, enzymatic bone powder, N-acetyl glucosamine and black pepper extract, which can significantly repair joint damage, significantly reduce cartilage damage indexes such as CTX-II and COMP, and can be used for preparing a joint maintenance medicine.

The invention discloses a functional composition capable of improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component, the first active component is prepared from elastin peptide, haematococcus pluvialis and ergothioneine; the second active component is prepared from one or more of herba houttuyniae and non-denatured type II collagen. Through scientific compatibility of all the components, the levels of glycosaminoglycan in chondrocytes and osteocalcin in osteoblasts are synergistically improved, and the protection effect on joint, cartilage and skeleton health is achieved. Meanwhile, a plurality of components in the functional composition capable of improving the health of the bones and the joints are natural in source, good in biocompatibility and free of obvious side effects, and a medicine or an alternative scheme thereof is provided for treatment and prevention of the bone diseases.

The invention relates to the technical field of biological pharmacy, and particularly discloses targeting drug-loaded nanoparticles for treating rheumatoid arthritis (RA) as well as a preparation method and application thereof, and the technical key points are as follows: on the basis that a GLP-1R agonist (such as liraglutide) is proved to be capable of effectively relieving rheumatoid arthritis symptoms, the targeting drug-loaded nanoparticles can be used for treating rheumatoid arthritis; gLP-1RA is wrapped by the silk fibroin nanoparticles to prepare the drug-loaded nanoparticles, and the drug-loaded nanoparticles can effectively improve the stability of GLP-1RA, prolong retention time in vivo and improve the curative effect of the drug; the drug-loaded nanoparticles and the type II collagen targeting peptide (WYRGRL) are crosslinked, so that the drug-loaded nanoparticles target the articular cartilage, the drug is gathered on the articular cartilage to be attached, the local concentration of the drug and the availability of the drug are further improved, and the curative effect of inhibiting the progress of RA is further enhanced; for lesion facet joints in RA, the treatment scheme of micro-needle administration can realize slow release of drugs, and the micro-needle is convenient to use and is suitable for patients to use at home.

The invention belongs to the technical field of biomedical materials, and particularly relates to biomimetic mineralized II-type collagen hydrogel as well as a preparation method and application thereof. The method comprises the following steps: gelling an acid solution of type II collagen in an alkaline environment to obtain type II collagen hydrogel; and dipping the type II collagen hydrogel in a mineralizing solution for mineralization to obtain the biomimetic mineralized type II collagen hydrogel. According to the method provided by the invention, the complex shape and nano-scale characteristics of the collagen fiber template can be accurately filled and copied, and the obtained biomimetic mineralized II-type collagen hydrogel can realize a tissue bionic effect. The biomimetic mineralized gel prepared by the invention has type I and type II amido bonds, the unmineralized collagenous fiber has a 67nm D-banding periodic structure, and hydroxyapatite crystals in the mineralized collagenous fiber are distributed along a long axis of collagen fibril composed of type II collagen, so that mineralization in the collagenous fiber is realized.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to a composition for treating osteoarthritis and a preparation method thereof. The composition for treating osteoarthritis is prepared from the following raw materials in parts by weight: 1 to 3 parts of non-denatured type II collagen, 0.3 to 0.5 part of cowherb seed cyclic peptide A and 0.4 to 0.7 part of balanosine. According to the composition, through the synergistic effect of multiple active ingredients, the osteoarthritis symptom is jointly relieved, and joint tissue repair is promoted.

The invention relates to the technical field of nutritional supplement preparations for pets, in particular to a high-activity non-denatured II-type collagen pet joint dispersible tablet and a preparation method thereof. According to the technical scheme, the soft capsule is prepared from the following raw materials in percentage by weight: 4.0%-7.0% of non-modified II type collagen, 4.0%-6.0% of sodium chondroitin sulfate, 4.0%-6.0% of green lip mussel powder, 0.5%-1.5% of sodium hyaluronate, 30%-40% of microcrystalline cellulose, 12%-18% of sodium carboxymethyl starch, 15%-22% of sweet potato starch, 4.0%-6.0% of polyvinylpolypyrrolidone, 6.0%-10.0% of enzymolysis chicken liver powder, 2.0%-4.0% of yeast extract, 1.5%-3.5% of lactose goat milk powder and 0.3%-0.7% of magnesium stearate. A core active component structure is protected by virtue of nano embedding and a whole-course low-temperature process, and a dual joint maintenance path of immunoregulation and nutrition supplement is constructed.

The invention belongs to the technical field of biological medicine, and discloses application of miRNA Let-7a-5p in a medicine for promoting cartilage formation, the invention provides the effect of miRNA let-7a-5p in cartilage formation / generation for the first time, and experiments prove that miRNA let-7a-5p can remarkably promote cartilage cell proliferation, enhance the activity of cartilage cells and remarkably improve matrix secretion of cartilage cells, so that the effect of promoting cartilage formation / generation is achieved. The expression of type II collagen is increased (the type II collagen is the main component of the cartilage matrix). Meanwhile, the miRNA let-7a-5p can also regulate other genes (such as NBL1 and DUSP7 genes) to inhibit cartilage ossification, and is expected to be applied to cartilage injury repair and osteoarthritis treatment, cartilage regeneration promotion and cartilage injury repair.

A method of forming a suspension of an undenatured type II collagen is provided, wherein the method comprises the steps of: (a) combining a natural flavouring and / or preserve and water, and heating in a first heating stage; (b) adding a gum; (c) adding a hydrated pectin mixture, and heating to a temperature higher than the temperature of the first heating stage; (d) adding an undenatured type II collagen powder; and (e) cooling to form a suspension. The method may further comprise added an acidifying agent, such as citric acid, after adding the undenatured type II collagen. The gum may be selected from xanthan gum and / or lecithin sunflower gum. A suspension of an undenatured type II collagen prepared by the method is provided. An exemplary suspension for dogs comprises 40 wt% chicory root fibre, 4 wt% undenatured type II collagen, 1.2 wt% pectin, 0.55 wt% citric acid, 0.4 wt% xanthan gum, 0.3 wt% natural flavouring, 0.05 wt% potassium sorbate, and 53.5 wt% water. The suspension is preferably a gel that can be orally administered to a pet or animal with joint pain / arthritis.

The invention relates to a composite nutritional composition and application thereof. The composite nutritional composition comprises the following components in parts by weight: 1 part of turmeric; 0.002 to 5 parts by weight of enzymatically hydrolyzed bone meal; and 0.02 to 0.2 part by weight of cartilage powder containing type II collagen or 0.02 to 10 parts by weight of collagen peptide powder. According to the compound nutritional composition provided by the invention, aiming at the particularity of bone and joint problems of sports people, the intervention effect of the compound nutritional composition provided by the invention on oxidative stress, inflammatory response and pain feeling related to sports is systematically evaluated by constructing an articular cartilage cell inflammation model and testing people; the invention aims to break through the limitation of a traditional general population formula, develop a precise nutrition scheme with component synergy and exercise physiology adaptability, and provide more scientific and effective joint health protection for exercise crowds.

Currently available non-denatured collagen products include powders and capsules that can optionally be incorporated into a final product that does not require heating or acidic conditions, or instead is consumed directly. This has been particularly problematic for administration to non-human mammals, such as pets and livestock, because when added as a dry powder to food or water, the supplements and capsules sink to the bottom of the bowl of food or liquid and remain unconsumed by the mammal, or in the case of capsules, can be difficult, if not impossible, to administer to the mammal.SOLUTION: The present disclosure relates to processed animal foods and / or treats comprising undenatured type II collagen. The undenatured type II collagen is introduced into the animal food and / or treat prior to processing and at least 30% or more of the undenatured collagen remains undenatured after processing of the animal food and / or treat.SELECTED DRAWING: None

The invention belongs to the technical field of biological medicines, and particularly relates to a drug-loaded nano-micelle as well as a preparation method and application thereof. According to the invention, bromoalkanes with different chain lengths are introduced to a leonurine phenolic hydroxyl group by adopting a total synthesis method to prepare a leonurine derivative, and the drug-loaded nano-micelle is prepared by applying triptolide (TP) and the leonurine derivative. The embodiment of the invention verifies that the drug-loaded nano-micelle can reduce in-vivo and in-vitro toxicity caused by triptolide and improve the treatment effect on a mouse arthritis model induced by type II collagen. The novel triptolide compatible medicine prepared by the invention is small in toxic and side effects and has a good curative effect on rheumatoid arthritis.

The invention discloses a food beneficial to bone movement health and a preparation method thereof, and the food comprises the following components by weight: 3-5 parts of bone collagen; 0.3 to 0.5 part of non-denatured type II collagen; 7-9 parts of a cartilage extract; 0.4 to 0.6 part of rhizoma curcumae longae; 1-3 parts of mangosteen fruit powder; 0.8 to 1.0 part of sodium hyaluronate; 0.1 to 0.3 part of N-acetylglucosamine; 0.4 to 0.6 part of milk mineral salt; 0.02 to 0.04 part of vitamin D3 micro-capsule powder; and 0.16 to 0.18 part of casein phosphopeptides. The food disclosed by the invention is beneficial to comprehensive maintenance of skeleton movement health, has no side effect and is high in popularization value.

The application relates to the technical field of animal models, and discloses a construction method and application of an ectopic ossification arthritis animal model. 91‑115 After being mixed and emulsified with chicken type II collagen as an antigen, animals are immunized to obtain the animal model, which is referred to as a C2V7 model. The arthritis model constructed by the application has high success rate, short time consumption and easily-obtained modeling materials. The model can simulate the onset process of arthritis with obvious ectopic ossification characteristics such as ankylosing spondylitis, enthesial-related arthritis and psoriatic arthritis, objectively reflects the pathological process and pathological characteristics of ectopic ossification arthritis, and provides a new research platform for related research.

The application discloses a composition for promoting joint health, a preparation method and application, and relates to the field of food.The composition comprises the following components in parts by weight: animal protein: 20-80; non-denatured type II collagen: 0.2-1; sodium hyaluronate: 0.5-2; proteoglycan: 0.5-2; sour cherry extract: 3-10; and calcium beta-hydroxy-beta-methylbutyrate: 5-20.The application takes non-denatured type II collagen as a main efficacy component, and additionally adds animal protein, sodium hyaluronate, proteoglycan, sour cherry extract and calcium beta-hydroxy-beta-methylbutyrate, synergistically acts through the multiple efficacy components, relieves joint discomfort through multiple pathways, maintains muscle health, and enables the product to have the optimal effect of repairing joint damage, improving joint and skeletal muscle health.

1. The name of the design product: packaging box (non-denatured type II collagen chitosan oligosaccharide compound tablet). 2. The use of the design product: for packaging health care products. 3. The design points of the design product: the combination of shape, pattern and color. 4. The picture or photo that best indicates the design points: front view. 5. The design for which protection is sought includes color. 6. The bottom of the design product is a part that is not easily visible or invisible during use, and the top view is omitted.

This invention relates to a method for constructing a humanized pig model of type II collagen and its application, belonging to the field of gene modification technology. The invention first provides a CRISPR-Cas9 system for targeted editing of the porcine type II collagen α1 gene. Using CRISPR / Cas9-mediated homologous recombination, the porcine COL2A1 gene is orally replaced with the human COL2A1 gene. Then, using somatic cell cloning technology, a humanized pig model of type II collagen is constructed, allowing the pig to produce cartilage tissue more similar to that of humans, reducing post-transplant immune rejection. This is expected to fundamentally solve the problem of human cartilage tissue shortage and has significant implications for the treatment of articular cartilage damage, providing a better and more suitable transplant material for the treatment of articular cartilage damage.

The invention belongs to the technical field of biological medicine, and provides a molecular mechanism for repairing degenerative arthritis cartilage through combination of non-denatured type II collagen and chitosan oligosaccharide and application of the molecular mechanism, the molecular mechanism comprises the non-denatured type II collagen and the chitosan oligosaccharide, and the mass ratio of the non-denatured type II collagen to the chitosan oligosaccharide is 1: 100-50: 1. The combination is used for repairing cartilage through the following molecular mechanisms: the non-denatured type II collagen retains a complete triple helix structure and induces regulatory T cell differentiation so as to inhibit release of proinflammatory factors; the chitosan oligosaccharide reduces cartilage cell apoptosis by inhibiting a p38MAPK pathway; the non-denatured type II collagen and the chitosan oligosaccharide are combined for use to synergistically activate a Wnt / beta-catenin pathway to promote the synthesis of the type II collagen and proteoglycan; according to the invention, non-denatured type II collagen and low-polymerization-degree chitosan oligosaccharide are combined for use, a stable composite system is formed through mediation of a flavonoid activator, a repair pathway is synergistically activated, and efficient, safe and long-acting repair of degenerative arthritis cartilage is realized in cooperation with optimization of a preparation and a process.