118 results about "Type II collagen" patented technology

The invention belongs to the technical field of microbial fermentation, and particularly relates to lactobacillus rhamnosus capable of maintaining bone health and improving local joint inflammation and a metagen and application of the lactobacillus rhamnosus. According to the invention, the probiotic NKU ML1-2 is subjected to fermentation culture, such that a post-generation product is prepared. Results of embodiments prove that the metagen product can enhance the oxidation resistance of cartilage and osteoblasts and promote the synthesis of anti-inflammatory factors and cartilage matrixes (type II collagen and proteoglycan), and has the potential of relieving oxidative stress and inflammatory response of local joints, relieving joint inflammation and delaying cartilage destruction. The prepared metagen product is high in safety and good in stability, and can be applied to preparation of medicines for preventing and / or treating osteoarthritis; the metagen product can also be used for promoting bone health, enhancing the antioxidant capacity of osteoblasts and / or chondrocytes, promoting osteoblast activity and / or promoting calcium absorption and fixation, and a new solution is provided for joint health management.

The invention provides a preparation method of non-denatured type II collagen capable of increasing bone mineral density and improving bone elasticity, and belongs to the technical field of biologication.The preparation method comprises the steps that an EDTA-citric acid buffer solution containing beta-mercaptoethanol is adopted for decalcifying cartilage, a supercritical CO2 extraction technology is adopted for degreasing, and the collagen is obtained; alkaline protease and collagenase II are adopted for substrate directional enzymolysis and gradient digestion; then the non-denatured type II collagen is separated by adopting three-phase centrifugal separation, and the non-denatured type II collagen with high extraction rate, high purity and high structural integrity can be obtained by adopting bionic H column chromatography purification.

This invention provides a composition for improving bone and joint health, its preparation method, and its application, relating to the field of bone and joint health products technology. By weight, the composition for improving bone and joint health comprises the following components: 0.5-5 parts collagen peptides, 5-30 parts mangosteen extract, 0.5-3 parts non-denatured type II collagen peptides, 0.1-2 parts milk mineral salts, 0.3-3 parts bone collagen peptides, 0.1-2 parts cartilage extract, 0.1-2 parts N-acetylglucosamine, 0.1-2 parts sodium hyaluronate, and 0.3-3 parts turmeric extract. This invention, through multiple combinations of mangosteen extract and turmeric extract, and cartilage extract and sodium hyaluronate, obtains a composition that is low in cost, highly safe, and has a good effect on the prevention or improvement of bone and joint diseases.

The invention discloses a preparation method of non-denatured II-type collagen for promoting bone and cartilage growth, and belongs to the technical field of II-type collagen extraction.The preparation method comprises the following steps that 1, pretreatment is conducted; (2) soaking in a salt solution: adding the salt solution into the cartilage particles for soaking treatment to obtain a precipitate; (3) soaking in an organic solvent; and (4) drying and crushing the solid matter to obtain the traditional Chinese medicine composition. The salt solution is prepared from the following components in percentage by mass: 1 to 5 percent of sodium chloride, 0.2 to 0.8 percent of lecithin, 0.3 to 1 percent of plant extract and the balance of water; the plant extract is a water extract of rhizoma kaempferiae, chicory and houttuynia cordata; the organic solvent is an ethanol water solution and pullulan polysaccharide. According to the method disclosed by the invention, the saline solution is improved, so that the structural stability of the collagen is improved while the dissolution of non-collagen components is promoted, the denaturation of the type II collagen is avoided, and the extraction efficiency of the non-denatured type II collagen is improved.

The invention belongs to the technical field of dairy product processing, and discloses camel milk composite nutrition powder beneficial to bone health, the camel milk composite nutrition powder is composed of the following raw materials by weight: 70%-75% of whole camel milk powder, 5%-15% of whey protein, 5%-20% of isomaltooligosacharide, 1%-10% of inulin, 0.02%-8% of milk mineral salt, 0.02%-5% of hydrolyzed yolk powder, and the balance water. The oral liquid is prepared from the following components in percentage by weight: 0.01%-0.5% of colostrum basic protein, 0.01%-1% of cartilage powder containing type II collagen, 0.01%-0.1% of N-acetylglucosamine, 0.01%-0.02% of sodium hyaluronate, 0.05%-5% of collagen peptide and 0.01%-1% of liquorice extract. According to the invention, a multi-dimensional health system is constructed by scientifically compounding a plurality of key components: milk mineral salt, colostrum basic protein and hydrolyzed egg yolk powder synergistically promote bone mineral density increase and bone metabolism balance; the II-type collagen cartilage powder, the N-acetylglucosamine and the sodium hyaluronate are combined to realize joint protection and skin repair; the six vitamins and the four minerals can accurately regulate and control the nutrition metabolism of the middle-aged and elderly people, especially can strengthen calcium absorption and oxidation resistance, and can significantly improve the bone health and improve the skin state.

Disclosed is use of a Histatin-1 polypeptide in promotion of cartilage regeneration or repair. By preparing a combined reagent containing a Histatin-1 polypeptide, a biological scaffold material and the like, mainly a linear Hst1 is loaded on gelatin methacrylate so as to be used for repairing cartilage damage or promoting cartilage growth; meanwhile, disclosed is use of a Histatin-1 polypeptide in promotion of the expression of one or more of bone differentiation indexes, including a collagen fiber, glycosaminoglycan, type II collagen and aggrecan.

The invention relates to a diatomic iron-iron site nano-enzyme constructed by relieving oxidative stress and cartilage degeneration and used for targeted osteoarthritis treatment. According to the invention, a nitrogen-doped porous carbon anchored diatomic iron nano enzyme catalyst (Fe2-NCs) with Fe-Fe dimer coordination is developed by using a'subject-object 'strategy. The Fe2-NCs protect cartilage cells from oxidative stress induced apoptosis by modulating ROS and active nitrogen species (RNS) and promoting O2 release. In addition, Fe2-NCs restores mitochondrial function by inhibiting NOX4 expression, improving ATP production, and normalizing COXIV levels. In an in-vivo OA model, the Fe2-NCs can reduce the expression of a pro-inflammatory medium COX-2 through an NF-kappa B signal channel, inhibit the up-regulation of MMP-13 and delay the degradation of type II collagen. The invention provides a new theoretical framework and methodological approach for the treatment of osteoarthritis, and has important clinical and scientific significance.

The invention provides a pharmaceutical composition for bone joints as well as a preparation method and application of the pharmaceutical composition. The pharmaceutical composition is prepared from the following components in parts by weight: 20 to 2000 parts of N-acetylglucosamine and 10 to 1000 parts of non-denatured type II collagen. In the compound composition of the N-acetylglucosamine and the non-denatured type II collagen, the two core components play a complementary role: the N-acetylglucosamine is focused on promoting synthesis of a cartilage matrix and provides a material basis for cartilage repair; the non-denatured type II collagen reduces damage of autoimmune response to cartilage through immunoregulation, the non-denatured type II collagen and the non-denatured type II collagen have a synergistic effect, the symptoms such as arthralgia and swelling can be relieved, the joint structure can be improved from the double angles of repairing and protecting, and particularly the treatment effect on patients with severe osteoarthritis is remarkably superior to that of a single-component preparation; and an innovative and efficient choice is provided for the treatment of osteoarticular diseases.

Disclosed herein are three-dimensional culture system for producing cartilage and / or selecting therapeutic agents for promoting cartilage regeneration.

The invention discloses a filling material for promoting tissue regeneration and a preparation method thereof. The filling material is prepared by using type II collagen as a microsphere wall material and hydroxyapatite as a core material and dispersing composite microspheres in type I collagen gel. The filling material for promoting tissue regeneration prepared by the invention is used for filling parts such as eyebrow lines, eyebrow subcutaneous support, zygomatic fat and cheek fat pads, lacrimal ducts, nasolabial folds, noses, lips, chin, mandible periosteum and the like, has an obvious mechanical support effect, continuously stimulates the formation and rearrangement of endogenous collagen on the basis of extrinsic collagen supply, and can be used for promoting tissue regeneration. The medical and aesthetic plastic requirements are met.

The application discloses a functional composition for improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component; the first active component comprises elastin peptide, haematococcus pluvialis and ergothioneine; and the second active component comprises one or more of houttuynia cordata and non-denatured type II collagen. The application realizes the protection effect on joint, cartilage and bone health by scientific matching of the components and synergistically improving the glycosaminoglycan level in chondrocytes and the osteocalcin level in osteoblasts. Meanwhile, the components in the functional composition for improving bone and joint health are natural and have good biocompatibility, and have no obvious side effects, so that the application provides a kind of drug or a substitute for the treatment and prevention of bone diseases.

The application provides a preparation method of non-denatured type II collagen for increasing bone density and improving bone elasticity, and belongs to the technical field of biotechnology. The application adopts EDTA-citric acid buffer containing beta-mercaptoethanol to decalcify cartilage, adopts supercritical CO2 extraction technology to defat, adopts alkaline protease and collagenase II to perform matrix directional enzymolysis, and performs gradient digestion; then three-phase centrifugal separation is adopted to separate non-denatured type II collagen, and biomimetic H column chromatography purification is adopted, so that non-denatured type II collagen with high extraction rate, high purity and high structural integrity can be obtained.

A method (10) of forming a gel suspension of an active ingredient such as undenatured type II collagen and / or a microbiome modulator. The method (10) comprising the steps of combining a natural flavouring and / or preserve and water, and heating in a first heating stage. The method (10) further comprises adding a thickening agent, e.g. xanthan gum. The method (10) further comprises adding a hydrated pectin mixture, and heating in a second heating stage. The second temperature being higher than the first temperature, preferably not exceeding 85 degrees Celsius. The method (10) further comprises adding the active ingredient(s) and rapid cooling to form a suspension.

The invention relates to the technical field of biological detection, in particular to an SERS (Surface Enhanced Raman Scattering) detection method of an osteoarthritis marker type II collagen. The SERS detection method of the osteoarthritis marker type II collagen comprises the following five steps: synthesizing gold nanoparticles, functionalizing an SERS substrate, coupling an SERS probe, establishing a detection standard curve and carrying out SERS spectrum detection. According to the method, the SERS signal of the osteoarthritis marker type II collagen is specifically enhanced through the plasmon resonance effect of the functionalized gold nanoparticles, trace detection of the type II collagen is achieved, detection can be achieved only by simply diluting or centrifuging a sample, purification and concentration steps needed by ELISA detection are avoided, a result is obtained within a short time, and the method is suitable for large-scale popularization and application. The kit is suitable for convenient and rapid diagnosis of early osteoarthritis; meanwhile, the invention provides an SERS (Surface Enhanced Raman Scattering) detection kit for the osteoarthritis marker type II collagen, which can be synthesized and stored on a large scale, so that the detection time is comprehensively shortened, and the detection efficiency is improved.

The present invention provides a method for quantitative analysis of type II collagen, a kit for the method, and peptides.

This invention relates to a compound nutritional composition and its application. The compound nutritional composition comprises the following components: 1 part by weight of turmeric; 0.002-5 parts by weight of enzymatically hydrolyzed bone powder; and 0.02-0.2 parts by weight of cartilage powder containing type II collagen or 0.02-10 parts by weight of collagen peptide powder. The compound nutritional composition provided in this application addresses the specific osteoarthritis issues of athletes. By constructing an articular cartilage cell inflammation model and conducting population testing, the intervention effects of the compound nutritional composition provided in this application on exercise-related oxidative stress, inflammatory response, and pain perception are systematically evaluated. The aim is to overcome the limitations of traditional broad-based formulations and develop precise nutritional solutions that combine component synergy with exercise physiological adaptability, providing athletes with more scientific and effective joint health protection.

This invention relates to the field of biomedical materials, providing non-denatured type II collagen ultrafine powder with cartilage tissue-targeted growth and structural repair functions, its bioactivity preservation preparation method, and its application in joints. The ultrafine powder has an average particle size of 0.5-50 μm, a triple helix structure integrity rate of not less than 85%, a thermal denaturation temperature of 38-48℃, and retains chondrocyte binding domains CB10, CB11, and MMP cleavage sites. The preparation method includes raw material pretreatment, time-limited enzymatic hydrolysis, graded purification, and ultra-low temperature ball milling. The product can be used for the repair and treatment of cartilage degenerative diseases such as osteoarthritis and cartilage damage.

The invention provides a glucosamine and chondroitin sulfate pharmaceutical composition as well as a preparation method and application thereof, and belongs to the technical field of biological pharmaceutical preparations, the composition comprises sodium chondroitin sulfate, glucosamine sulfate, dimethyl sulfone, non-denatured type II collagen, frankincense extract and auxiliary materials in specific parts by mass, compared with the prior art, the composition provided by the invention has the advantages that by selecting the components, adding the composite synergist optionally and optimizing the ratio and synergistic effect of the components, the effects of the composition in promoting cartilage regeneration and inhibiting joint inflammation are remarkably improved, the anti-inflammatory and anti-oxidation effects are enhanced, and the bioavailability is also improved, so that a breakthrough progress is realized in the field of joint health.

The application provides a composition containing curcuma and type II collagen-containing cartilage powder and application thereof in joint maintenance, and relates to the field of food technology. The application provides a composition containing milk mineral salt, curcuma, type II collagen-containing cartilage powder, enzymatic bone powder, N-acetyl glucosamine and black pepper extract, which can significantly repair joint damage, significantly reduce cartilage damage indexes such as CTX-II and COMP, and can be used for preparing a joint maintenance medicine.

The present invention discloses the use of Histatin-1 polypeptide in promoting cartilage regeneration or repair. By preparing a combined reagent composed of Histatin-1 polypeptide, a biological scaffold material, etc., mainly by loading linear Hst1 on gelatin methacrylate, it is used for cartilage injury repair or promoting cartilage growth. At the same time, the use of Histatin-1 polypeptide in promoting the expression of one or more of the bone differentiation indicators, including collagen fibers, glycosaminoglycans, type II collagen, and aggrecan, is also disclosed.

The invention relates to the technical field of biology, and particularly discloses a composition and application thereof in improving articular cartilage health. The composition comprises sodium hyaluronate, colostrum basic protein and N-acetylglucosamine, wherein the mass ratio of the sodium hyaluronate to the colostrum basic protein to the N-acetylglucosamine is (1-2): (1-2): (1-8). The composition disclosed by the invention can be used for increasing the bone mineral density, increasing the number of osteoblasts, promoting cartilage growth and increasing the content of II-type collagen in an animal body, so that the bone joint function can be effectively improved, and a new method is provided for joint maintenance.

The invention discloses a functional composition capable of improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component, the first active component is prepared from elastin peptide, haematococcus pluvialis and ergothioneine; the second active component is prepared from one or more of herba houttuyniae and non-denatured type II collagen. Through scientific compatibility of all the components, the levels of glycosaminoglycan in chondrocytes and osteocalcin in osteoblasts are synergistically improved, and the protection effect on joint, cartilage and skeleton health is achieved. Meanwhile, a plurality of components in the functional composition capable of improving the health of the bones and the joints are natural in source, good in biocompatibility and free of obvious side effects, and a medicine or an alternative scheme thereof is provided for treatment and prevention of the bone diseases.

The invention relates to the technical field of biological pharmacy, and particularly discloses targeting drug-loaded nanoparticles for treating rheumatoid arthritis (RA) as well as a preparation method and application thereof, and the technical key points are as follows: on the basis that a GLP-1R agonist (such as liraglutide) is proved to be capable of effectively relieving rheumatoid arthritis symptoms, the targeting drug-loaded nanoparticles can be used for treating rheumatoid arthritis; gLP-1RA is wrapped by the silk fibroin nanoparticles to prepare the drug-loaded nanoparticles, and the drug-loaded nanoparticles can effectively improve the stability of GLP-1RA, prolong retention time in vivo and improve the curative effect of the drug; the drug-loaded nanoparticles and the type II collagen targeting peptide (WYRGRL) are crosslinked, so that the drug-loaded nanoparticles target the articular cartilage, the drug is gathered on the articular cartilage to be attached, the local concentration of the drug and the availability of the drug are further improved, and the curative effect of inhibiting the progress of RA is further enhanced; for lesion facet joints in RA, the treatment scheme of micro-needle administration can realize slow release of drugs, and the micro-needle is convenient to use and is suitable for patients to use at home.

The invention belongs to the technical field of biomedical materials, and particularly relates to biomimetic mineralized II-type collagen hydrogel as well as a preparation method and application thereof. The method comprises the following steps: gelling an acid solution of type II collagen in an alkaline environment to obtain type II collagen hydrogel; and dipping the type II collagen hydrogel in a mineralizing solution for mineralization to obtain the biomimetic mineralized type II collagen hydrogel. According to the method provided by the invention, the complex shape and nano-scale characteristics of the collagen fiber template can be accurately filled and copied, and the obtained biomimetic mineralized II-type collagen hydrogel can realize a tissue bionic effect. The biomimetic mineralized gel prepared by the invention has type I and type II amido bonds, the unmineralized collagenous fiber has a 67nm D-banding periodic structure, and hydroxyapatite crystals in the mineralized collagenous fiber are distributed along a long axis of collagen fibril composed of type II collagen, so that mineralization in the collagenous fiber is realized.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to a composition for treating osteoarthritis and a preparation method thereof. The composition for treating osteoarthritis is prepared from the following raw materials in parts by weight: 1 to 3 parts of non-denatured type II collagen, 0.3 to 0.5 part of cowherb seed cyclic peptide A and 0.4 to 0.7 part of balanosine. According to the composition, through the synergistic effect of multiple active ingredients, the osteoarthritis symptom is jointly relieved, and joint tissue repair is promoted.

The invention relates to the technical field of nutritional supplement preparations for pets, in particular to a high-activity non-denatured II-type collagen pet joint dispersible tablet and a preparation method thereof. According to the technical scheme, the soft capsule is prepared from the following raw materials in percentage by weight: 4.0%-7.0% of non-modified II type collagen, 4.0%-6.0% of sodium chondroitin sulfate, 4.0%-6.0% of green lip mussel powder, 0.5%-1.5% of sodium hyaluronate, 30%-40% of microcrystalline cellulose, 12%-18% of sodium carboxymethyl starch, 15%-22% of sweet potato starch, 4.0%-6.0% of polyvinylpolypyrrolidone, 6.0%-10.0% of enzymolysis chicken liver powder, 2.0%-4.0% of yeast extract, 1.5%-3.5% of lactose goat milk powder and 0.3%-0.7% of magnesium stearate. A core active component structure is protected by virtue of nano embedding and a whole-course low-temperature process, and a dual joint maintenance path of immunoregulation and nutrition supplement is constructed.

The invention discloses an application of a cathepsin D inhibitor in preparation of a medicine for preventing or treating osteoarthritis, and an application of the cathepsin D inhibitor in preparation of a medicine for inhibiting degradation of type II collagen and / or articular cartilage. The invention relates to an application of a cathepsin D inhibitor in preparation of drugs for inhibiting expression of cathepsin D in bone joints. In the application, the cathepsin D can effectively relieve tissue lesions of osteoarthritis.

The present disclosure provides recombinant type II humanized collagen and uses thereof. The recombinant II-type humanized collagen has a typical triple helix structure and excellent biological characteristics, can be used for cartilage repair, and does not generate immunological rejection and anaphylactic reaction when being applied to a human body.

The invention belongs to the technical field of biological medicine, and discloses application of miRNA Let-7a-5p in a medicine for promoting cartilage formation, the invention provides the effect of miRNA let-7a-5p in cartilage formation / generation for the first time, and experiments prove that miRNA let-7a-5p can remarkably promote cartilage cell proliferation, enhance the activity of cartilage cells and remarkably improve matrix secretion of cartilage cells, so that the effect of promoting cartilage formation / generation is achieved. The expression of type II collagen is increased (the type II collagen is the main component of the cartilage matrix). Meanwhile, the miRNA let-7a-5p can also regulate other genes (such as NBL1 and DUSP7 genes) to inhibit cartilage ossification, and is expected to be applied to cartilage injury repair and osteoarthritis treatment, cartilage regeneration promotion and cartilage injury repair.