Cartilage targeting double-drug-loaded nano-microspheres with optical thermal response characteristic and preparation method and application thereof

Abstract

The invention relates to a preparation method and application of cartilage targeting double-drug-loaded nano-microspheres with the optical thermal response characteristic. According to thecartilage targeting double-drug-loaded nano-microspheres with the optical thermal response characteristic, small-particle-size mesoporous polydopamine is used as a matrix, the matrix is sequentially subjected toa cyclic reaction with FeCl3. 6H2O and H3BTC, and the surface of the mesoporous polydopamine is modified to form a metal organic framework. After the synthesized MPDA-MOF (referred to as MPM hereinafter) nano-microspheres react with EDC and NHS solution, carboxyl groups on the surface of MOF are activated, and by connection with amino groups in type II collagen targeting peptide (WYRGRL), composite nano-microspheres (MPMW) of which surfaces are loaded with a cartilage targeting peptide are formed. Double factors of bilirubin (Br) and rapamycin (Rap) are correspondingly loaded in pore passagesof an MOF shell layer and the mesoporous polydopamine, and finally, the RB@MPMW is obtained. The nano-microspheres have the advantages of high optical thermal response characteristic, good biocompatibility, active cartilage targeting, delay on in-vivo cartilage degeneration and the like, and can be used for the targeted therapy of osteoarthritis cartilage degeneration.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a double drug-loaded nano-microsphere targeting cartilage with photothermal response characteristics, a preparation method and application thereof. Background technique [0002] Osteoarthritis (OA) is a chronic degenerative disease of cartilage. Due to the lack of nerves and blood vessels, articular cartilage has very poor proprioception of pain. After the cartilage is damaged, its self-repair ability will be further reduced, which will further aggravate the damage. Previous studies have shown that physical and drug interventions in the early stages of cartilage degeneration can prevent the progression of OA to a certain extent. Therefore, the use of imaging techniques to diagnose early osteoarthritis and implement effective interventions is an important step for the comprehensive treatment and prevention of OA. An effective solution is provided. Several therape...

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Problems solved by technology

[0003] When chondrocytes are stimulated by a variety of external inflammatory factors, mechanical stress and other factors, the level of intracellular oxidative stress is often at a high level, and the level of intracellular reactive oxygen species is therefore high; and previous studies have shown that antioxidants play a role in the treatment of OA. has great potential, but some oxygen radical scavengers exhibit potential cytotoxicity, which also limits its further application

Recent studies have found that bilirubin, one of the metabolites of bile acids, has multiple biological functions, including free radical scavenging, but its efficacy in OA treatment is still unknown

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