Preparation method of cartilage extract containing non-denatured type II collagen

A collagen and extract technology, used in chemical instruments and methods, animal/human proteins, connective tissue peptides, etc., can solve problems such as pretreatment, low extraction rate, and limited storage conditions, and achieve easy large-scale production and extraction. High-efficiency, easy-to-save effect

Inactive Publication Date: 2017-07-04
BEIJING SEMNL BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there are some extraction processes of non-denatured type II collagen at home and abroad, but either the raw materials have not been pretreated to remove impurities, or the impurities have not been treated, or the extraction rate is low, or the storage conditions are limited.

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  • Preparation method of cartilage extract containing non-denatured type II collagen
  • Preparation method of cartilage extract containing non-denatured type II collagen

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preparation example Construction

[0012] The invention provides a method for preparing a cartilage extract containing non-denatured type II collagen, which is characterized in that the method comprises the following steps in sequence: degreasing, disinfection, homogenization, enzymolysis, filtration, and drying; wherein, in the In the enzymatic hydrolysis step: adjust the pH of the slurry obtained in the homogenization step to 2.5-8.5, add 0.001%-2% of the cartilage weight of enzyme, add 1 / 20-1 / 500 of the cartilage weight of papaya And / or the clear liquid obtained by filtering the pineapple juice at low temperature, enzymatic hydrolysis for 12-48 hours, and the enzymatic hydrolysis temperature is 25-50°C.

[0013] The inventors of the present application unexpectedly found that a higher concentration of non-denatured type II collagen can be obtained by enzymatic hydrolysis under the above-mentioned enzymatic hydrolysis conditions.

[0014] In the enzymolysis step, the enzyme can use any enzyme used in the art,...

Embodiment 1

[0033] (1) Pretreatment: Select 3.5 kg of chicken breast cartilage, remove the chicken meat, fascia and red bone on the cartilage after thawing, and then cut into cubes of 3*3mm by a dicing machine.

[0034] (2) Degreasing: use a final concentration of 0.01% Na 2 CO 3 and 2% NaCl mixed degreasing agent solution 7kg, degreasing at room temperature for 4 hours, after degreasing and draining, wash with purified water to pH 6.5.

[0035] (3) Disinfection: add sodium hypochlorite solution with a concentration of 400ppm to start disinfection, and disinfect twice, each time for 12 hours. After disinfection, wash with purified water until no disinfection and no residual chlorine is detected, and the pH is controlled at 6.5-7.0.

[0036] (4) Homogenization: Add 6 kg of purified water to the cartilage dices treated in step (3), and grind the cartilage into 100-200 meshes with a colloid mill.

[0037] (5) Enzymolysis: adjust the pH of the slurry obtained in step (4) to 2.5, add 70 gram...

Embodiment 2

[0043] (1) Pretreatment: Select 20kg of fresh duck breast cartilage, remove the duck meat, fascia and red bone on the cartilage after thawing, and then use a dicing machine to cut into cubes of about 2mm*2mm.

[0044] (2) Degreasing: Use 200 kg of NaOH and 0.9% KCl solution with a final concentration of 0.01%, degrease at room temperature for 6 hours, and then wash with purified water to pH 6.0.

[0045] (3) Disinfection: Add 500ppm sodium hypochlorite solution (containing 100ppm hydrogen peroxide) to start disinfection, disinfect 3 times, each time for 6 hours, after disinfection, wash with purified water until no disinfection and no residual chlorine is detected, and the pH is controlled at 6.5-7.0 .

[0046](4) Homogenate: Add 40 kg of purified water to the cartilage dices processed in step (3), and use a colloid mill to grind the cartilage into about 100-200 mesh.

[0047] (5) Enzymolysis: adjust the pH of the slurry obtained in step (4) to 8.5, add 0.2 g of alkaline prot...

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Abstract

The invention provides a preparation method of cartilage extract containing non-denatured type II collagen. The method comprises the following steps: degreasing, disinfecting, homogenizing, enzymatic hydrolysis, filtration and drying. In the enzymatic hydrolysis step, the pH of a slurry obtained in the homogenizing step is adjusted to 2.5-8.5, an enzyme accounting for 0.001-2%of the weight of cartilages is added, a clear liquid accounting for 1/20 to 1/500 of the weight of the cartilages and obtained through juicing pawpaw and/or pineapples and filtering the obtained juice is added, the enzymatic hydrolysis is carried out at 25-50 DEG C for 12-48 h, and the enzyme is preferably selected from one or more of pepsin, subtilisin, alkaline proteases and metalloproteases. The cartilage extract obtained through the preparation method has the advantages of high purity, high extraction rate, and easiness in large-scale production, and richness in non-denatured type II collagen and chondroitin sulfate.

Description

technical field [0001] The invention relates to a preparation method of cartilage extract, in particular to a preparation method of cartilage extract containing non-denatured type II collagen. Background technique [0002] Collagen is an important component of tissues such as skin, bones and joints, and is the main component of the extracellular matrix of multicellular animals. At present, 27 different types of collagen have been released from mammals, among which type II collagen composed of three identical α chains mainly exists in cartilage tissue and in the vitreous body of the eye, so type II collagen is regarded as a part of cartilage A structural functional element. [0003] At present, the collagen sold in the market is mostly hydrolyzed collagen, the main component of which is polypeptide, and the molecular weight distribution is in the range of 200-20000Da. Although these polypeptides also have certain functions of maintaining skin moisture, enhancing bone densit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C08B37/08
CPCC12P21/06C07K14/78C08B37/0003C08B37/0069
Inventor 刘爱青王海燕张贵锋刘爽
Owner BEIJING SEMNL BIOTECHNOLOGY CO LTD
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