37 results about "Multicellular animals" patented technology

The present invention relates to systems and methods for quantitative three-dimensional mapping of refractive index in living or non-living cells, tissues, or organisms using a phase-shifting laser interferometric microscope with variable illumination angle. A preferred embodiment provides tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure and the quantitative characterization of specimen-induced aberrations in high-resolution microscopy with multiple applications in tissue light scattering.

The invention relates to a non-human multicellular organism carrying a dominant lethal genetic system, the lethal effect of which is conditional, wherein the lethal effect of the lethal system occurs in the natural environment of the organism.

The present invention is directed to method of modulating a heat shock response in a first cell of a multicellular organism comprising stimulating or inhibiting an HSR signaling activity of a second cell, wherein the second cell is a neuronal cell that regulates heat shock response activation in the first cell and that does not directly innervate the first cell.

The invention concerns a capsule containing cells that are transiently transfected with a gene of interest and entrapped within a biocompatible polymer membrane, a method of preparing those capsules, a method for in vivo assessing an activity of the protein secreted by the gene of interest, which comprises administering to a multicellular organism the capsule and detecting an activity, a pharmaceutical composition comprising such a capsule, and the use thereof for the administration of a protein of interest to a subject.

Methods for homologously recombining an exogenous nucleic acid into a target cell genome of a multicellular organism, e.g., an animal, are provided. In the subject methods, a targeting vector that includes a linearizing endonuclease site, e.g., a recombinase recognition site, and a homologous recombination integrating element, is contacted with the multicellular organism, e.g., via systemic or local administration, such that the target cell(s) of the multicellular organism takes up the targeting vector. The targeting vector is one that has been linearized by a linearizing endonuclease, e.g., a recombinase, at some point prior to the homologous recombination event, e.g., prior to or after contact with the multicellular organism. Following uptake by the target cell(s), the integrating element then homologously recombines into the target cell genome from the linearized targeting vector. Also provided are targeting vectors, systems and kits for use in practicing the subject methods.

This disclosure relates to analyzing the end-to-end sequence and the relative distributions in heterogeneous mixtures of polynucleotides and methods and enabling reagents related thereto. In certain embodiments this method relates to the complete full length sequencing and quantitative profiling of mRNAs present in the transcriptomes of cells or tissues of, but not limited to, higher multicellular organisms that possess interrupted genes subject to complex post-transcriptional RNA processing.

The invention is related to a method and a complex of DNA constructs for providing an increased level of control of transgene segregation in Sexually Reproducing Multicellular Organisms (SRMOs), which are prone to out-crossing with their wild-type or cultivated relatives. The method and constructs allow the farmer to reuse the transgenic crop. without risk. The RBF system comprises one or more Transgenes of Interest (TGIs) encoding desired gene products, one or more Blocking Constructs (BC), and one or more user-controlled means for recovering the blocked functions. The BC has the capacity of blocking at least one function essential for the survival and/or reproduction of the SRMO. Preferably more than one BC flanking the TGIs are used. The BC may be located in close proximity to the TGI, preferably in an intron or flanking the TGIs. The blocked function is recoverable by user-controlled interventions preferably applicable under confined conditions. The intervention is combined with one or more Recovering Constructs (RC). Different types of RBF systems are disclosed. The Double and Triple and Segregating RBF systems and intron-inserted BC are the preferred embodiments of the present invention. The Transgenic Inserts (TI) of the Triple RBF system and their interactions are shown in FIG. 10.

Methods for removing endotoxin from naturally occurring materials, such as polysaccharides (e.g., agarose and/or carrageenan) are described herein. Polysaccharides that are substantially free of endotoxins and uses thereof are also described. The polysaccharide materials can be isolated from microorganisms, multicellular organisms, such as, algae, plants, seaweed, etc. The method involves the use of acidic and basic solutions to hydrolyze the lipid-polysaccharide bond in endotoxins. Cleaving the fatty acid from the polysaccharide reduces the water-solubility of the fatty acid and enables its removal with an organic solvent such as ethanol. The polysaccharide component can also undergo acidic or basic hydrolysis due to the weak glycosidic bond between the sugar rings.

The invention relates to a deep-sea organism capturing and culturing technology, and aims to provide a deep-sea multicellular organism pressure-maintaining capturing and long-term culture device. The two ends of a pressure maintaining cavity of the device are open, and movable end covers are arranged on the inner side for sealing; a spring sleeve mechanism is arranged in the pressure maintaining cavity in parallel, an inner sleeve and an outer sleeve are in coaxial interference fit, and the two ends of a spring are fixed to a movable end cover. The outer side of the movable end cover is connected with a damping release mechanism; the pressure maintaining cavity is further connected with an energy accumulator system, a fresh seawater conveying system and a food feeding system. By adopting a sealed triggering structure, the structure of the biological pressure-maintaining sampling equipment is simplified, the weight is reduced, and the operation is simple and convenient. The two ends of the pressure maintaining cabin are sealed by the movable end covers, and the movable end covers are powered by the spring sleeves, so that the design of a motor, an oil cylinder and a transmission structure which are possibly added is omitted, the overall structure is simplified, and the cost is saved. The matched culture system can provide seawater circulation renewal, oxygen supply and physical nutrition supply for multicellular organisms.

The present invention provides a method of transcriptionally modulating the expression of a gene of interest, the expression of which is associated with a defined physiological or pathological effect within a multicellular organism. The method comprises contacting a cell which is capable of expressing the gene with an amount of a molecule effective to transcriptionally modulate expression of the gene and thereby affect the level of the protein encoded by the gene which is expressed by the cell. Molecules useful in the practice of the invention are characterized as follows (a) do not naturally occur in the cell, (b) specifically transcriptionally modulate expression of the gene of interest, and (c) bind to DNA or RNA or bind to a protein through a domain of such protein which is not a ligand binding domain of a receptor which naturally occurs in the cell, the binding of a ligand to which ligand binding domain is normally associated with the defined physiological or pathological effect.

The present invention provides methods of protecting plants and other multicellular organisms from the parasitic activity of nematodes and insects. The methods use compounds, such as stilbene compounds, as nematicidal and insecticidal compounds, which act to alter the activity of one or more anion transporters in the nematodes and insects. Methods of screening for such compounds are also provided.

The present invention relates to molecular probes of the formula (I)

L1-R1-L-A-X  (I)

as defined herein that allow for the observation of the catalytic activity of a selected caspase, cathepsin, MMP and carboxypeptidase in in vitro assays, in cells or in multicellular organisms, a method for their preparation and the use thereof.

A method of promoting increased cellular hydration in a multicellular organism that is capable of intracellular water permeation includes the step of causing the multicellular organism to ingest an aqueous solution that contains an amount of a carbohydrate clathrate component. There is also a step of enhancing the intracellular permeation. The multicellular organism contains aquaporins, and the causing step involves interaction of the composition with the aquaporins. The cellular hydration promoted and caused by the method is corroborated by a test that uses human-aquaporin-expressed frog oocytes. The test uses single cell Xenopus laevis frog oocytes having expressed human aquaporin AGP1 water channels. There is also a beverage composition that increases lifespan in the multicellular organism, and a beverage composition that promotes cellular hydration when ingested by a multicellular organism.

The present invention relates to a feed additive formula and relates to a feed additive for improving immunity of broiler chickens. The feed additive is characterized by being prepared from the following components in parts by weight: (4 plus or minus 0.5) parts of vitamin A acetate, (4 plus or minus 0.5) parts of vitamin D3, (4 plus or minus 0.5) parts of DI alpha-tocopheryl acetate, (4 plus or minus 0.5) parts of vitamin B1, (4 plus or minus 0.5) parts of vitamin B2, (4 plus or minus 0.5) parts of vitamin B6, (4 plus or minus 0.5) parts of vitamin B12, (4 plus or minus 0.5) parts of menadione nicotinamide bisulfite, (4 plus or minus 0.5) parts of niacinamide, (4 plus or minus 0.5) parts of folic acid, (4 plus or minus 0.5) parts of D-calcium pantothenate, (4 plus or minus 0.5) parts of D-biotin, (4 plus or minus 0.5) parts of choline chloride, (4 plus or minus 0.5) parts of copper sulfate, (6 plus or minus 0.5) parts of calcium hydrogen sulfate, (6 plus or minus 0.5) parts of ferroussulfate, (6 plus or minus 0.5) parts of manganese sulfate, (6 plus or minus 0.5) parts of zinc sulfate, (10 plus or minus 0.5) parts of calcium hydrogen phosphate and (10 plus or minus 0.5) parts ofstone powder. The feed additive belongs to the technical field of animal feeds. Immunization is an efficient and conserved mechanism used by multicellular animals to identify and prevent infection byexogenous pathogens and plays an important role in preventing inflammation and infection.