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High surface cultivation system with surface increasing substrate

A substrate, inner surface technology, applied in tissue cell/virus culture devices, solid-phase fermentation bioreactors, biochemical instruments, etc., can solve the problem of no significant benefits of suspension cell culture, increase in cell number, and lack of surface area to ensure culture at the same time. problems with adequate supply of bases, gases and other compounds

Inactive Publication Date: 2010-02-10
CINVENTION AG
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  • Summary
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  • Application Information

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Problems solved by technology

However, the increase in surface area is especially beneficial for adherent growing cells, but not for suspension cell cultures
[0006] In addition, conventional solutions are based on increased surface area but do not at the same time ensure adequate supply of media, gases and other compounds
It has been found that an increase in surface area alone results in a limited increase in cell number

Method used

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  • High surface cultivation system with surface increasing substrate
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Embodiment Construction

[0039] The present inventors have found that, in order to overcome the disadvantages of the prior art, and in particular to increase the active surface available for gas and liquid medium exchange in cell culture vessels, it is highly desirable to provide a substrate with increased surface area in the vessel.

[0040]In one embodiment the present invention provides an efficient cell culture vessel for cell and / or tissue culture comprising at least one reversibly closable well in the vessel wall and at least one surface area increasing substrate within the vessel, The base is made of a single module.

[0041] The container comprises at least one single modular base, preferably having a geometry selected from the group consisting of plate, disc, disc, cube, cylinder, tube, sphere, y-shape and star. Additionally, the mono-module substrate may be flat in at least one plane (ie, on one of its surfaces). The single-module base may have substantially the same net shape as the cultur...

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Abstract

The present invention is directed culture vessel suitable for cultivation of cells and / or tissues comprising at least one reversibly clo sable aperture in the vessel wall, and at least one surface-increasing substrate within the vessel, said substrate being made of a single mold. The invention further provides a system comprising at least two vessels being interconnected via at least one aperturein their vessel wall, and a cultivation process using such a vessel or system, in which at least one type of cells, tissue, tissue-like cell cultures, organs, organ- like cell cultures, or multicellular organisms are cultivated in the presence of at least one fluid or solid medium necessary for growing and / or cultivating the aforesaid culture.

Description

technical field [0001] The present invention relates to a new type of culture vessel, in particular to a culture vessel comprising a substrate of increased surface area in said vessel, said base being made of a single module and optionally means for convection of fluids, and to the use of said vessel method. In addition, the present invention relates to a culture system in which culture vessels are arranged in communication with each other. Background technique [0002] Culture vessels, such as spinner bottles, are widely used in cell culture, especially the culture of mammalian cells. The main applications are cell growth, production of cell products or virus particles. Common methods involve high-density cell culture, co-cultivation, infection of cells, and processing of samples for dialysis. Typically, a culture vessel such as a spinner bottle is a cylindrical vessel that enables rotation of the bottle about its longitudinal axis. The flasks are filled with a liquid m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/16C12M3/04
CPCC12M27/22C12M23/08C12M27/20C12M27/12
Inventor 苏海尔·阿斯加里
Owner CINVENTION AG
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