204 results about "Cell organisation" patented technology

The present invention, among other things, provides technologies for detecting and / or quantifying nucleic acids in cells, tissues, organs, or organisms. Pre-designed barcodes are associated with specific molecular targets through sequential hybridization experiments. A pseudo-color based barcoding scheme is described that overcomes the limitations in the previous generation of the technology. The current method can be applied to both in vitro and in situ analysis.

The invention discloses a preparation method of DNA (Deoxyribonucleic Acid) hydrogel with programmable mechanical strength. By regulating and controlling the molar ratio of the catalytic initiating chains to the total amount of the DNA monomer building blocks, multiple hydrogels which are equivalent in mass concentration and have different mechanical strengths are constructed. According to the method, a strategy similar to catalytic assembly is adopted, the feeding molar ratio of a catalytic initiating chain to a DNA monomer building block is creatively adjusted, the proportion of cross-linking reactions at different levels is regulated and controlled, then the DNA hydrogel with different mechanical strengths is synthesized under the isothermal condition, and the storage modulus range is about 200-2000 Pa. In addition, the change of the mechanical strength is independent of the total concentration of the matrix (DNA) of the hydrogel, the DNA sequence, the stress relaxation rate and other properties. The method provides a novel convenient method for constructing DNA hydrogels with different mechanical strengths, and possibly provides a novel tool for researching the specific influence of mechanical properties of materials on cell / tissue behaviors.

The utility model relates to the technical field of biological tissue engineering, in particular to an organ chip platform integrated with optical tweezers, which is used for assembling and culturing three-dimensional cell tissues and comprises an organ chip with optical transparency and holographic optical tweezers integrated with the organ chip. The organ chip is horizontally arranged and comprises an upper-layer plate, a middle-layer plate and a lower-layer plate which are sequentially stacked, the middle-layer plate is provided with at least two first culture pools used for inducing and differentiating stem cells into different types of cells and at least one second culture pool used for assembling and culturing the different types of cells, and the lower-layer plate is provided with at least two second culture pools used for assembling and culturing the different types of cells. The channel is connected with the first culture pond and the second culture pond; and an objective lens of the holographic optical tweezers faces the top surface or the bottom surface of the organ chip and is used for capturing, moving and assembling cells in the first culture pool or the second culture pool. According to the utility model, the dependence on a micro-pattern processing technology can be reduced, the cost is reduced, and the regulation and control capability on an organoid tissue structure is improved.

Methods and compositions for modulating a target genome are disclosed. This disclosure relates to novel compositions, systems and methods for altering a genome at one or more locations in a host cell, tissue or subject, in vivo or in vitro. In particular, the invention features compositions, systems and methods for inserting, altering, or deleting sequences of interest in a host genome.

Compositions, methods and systems for targeted delivery using one or more shuttles operably linked to one or more cargos (e.g., cargo polypeptides, e.g., cargo oligonucleotides) and subsequent quantification of an abundance of one or more cargos (e.g, proteins) in a mixture (e.g., a complex mixture (e.g., in vivo)) or in a specific cell, tissue, or organ of interest (e.g., in vivo) using barcodes (e.g., peptide barcodes), binders (e.g., polypeptide binders), and binding agents (e.g., phage) are provided herein.

The invention discloses a large model and multi-mode fused lysine modification site prediction method and device, relates to the technical field of biological information, and mainly aims to solve the problem of poor prediction effectiveness of lysine modification sites in existing proteins. Comprising the following steps: acquiring amino acid sequence information of cell tissue protein, and processing the amino acid sequence information based on a protein large language model which completes model training to obtain sequence representation; obtaining amino acid atom information of the cell tissue protein, and processing an amino acid contact graph structure constructed based on the amino acid atom coordinate information based on a graph structure model of which model training is completed to obtain structural characterization; and splicing the sequence representation and the structure representation, and performing feature fusion prediction on a representation vector obtained after splicing based on a multi-modal fusion model of which model training is completed to obtain a lysine modification site prediction result.

The invention relates to a pathological image spatial feature extraction method and an electronic device, and the method comprises the steps: respectively obtaining image block features and cell features from a region of interest, carrying out the spatial modeling of the image block features and the cell features, and respectively extracting tissue pathological features and cell pathological features based on a cell map and a tissue map; and fusing the two to obtain a final pathological feature. A cell map and a tissue map are respectively constructed from two perspectives of cells and tissues, the cell map properly contains distribution and interaction of the cells, the tissue map properly encodes a tissue microenvironment including tissue topology distribution which cannot be summarized by the cell map, and finally spatial information contained in the cell map and the tissue map is fused. Therefore, cell and tissue information in the pathological image is fully utilized, compared with a network which only uses a cell map or a tissue map, rich information in the pathological image can be better captured by simultaneously using the two types of maps, and the purpose of fully utilizing multi-scale space information in the pathological image can be achieved.

The invention relates to the technical field of culture devices, and discloses a fan regulation and control method for an incubator, which comprises the following steps: acquiring a temperature rise stage and an inner chamber temperature of the incubator and an environment temperature of an environment where the incubator is located; under the condition that the inner chamber temperature does not meet the first inner chamber temperature condition, according to the temperature rising stage of the incubator, a circulating fan is controlled to operate according to the temperature rising air speed; and after the inner chamber temperature meets the first inner chamber temperature condition, the temperature rising air speed of the circulating fan is adjusted according to the environment temperature. According to the method, the problem of back drop after temperature overshoot after the incubator is heated for the first time can be solved, the temperature fluctuation range of the temperature in the inner chamber is controlled, and the culture effect of cells / tissues in the incubator is improved. The invention further discloses a fan regulation and control device for the incubator, the incubator and a storage medium.

An ultra-high-cell-density multicellular tissue lifting bioprinting device assisted by oblique incident bulk wave and a printing method therefor are provided, and the printing device includes a printing liquid tank, a printing platform, a light source and an oblique incident bulk wave chip; the printing liquid tank contains a printing liquid which is a mixture of cells and a photocurable bioink; the printing platform is arranged horizontally and extends into the printing liquid tank to come into contact with the printing liquid; the printing platform is capable of going up and down; the light source is arranged under the printing liquid tank, and light enters the printing liquid tank; the oblique incident bulk wave chip is a piezoelectric transducer arranged obliquely; the piezoelectric transducer is arranged under the printing liquid tank and tilted relative to the horizontal plane to generate an oblique incident bulk wave acoustic field in the printing liquid.

The invention provides a cell data processing method and device, equipment, a storage medium and a program product. The method comprises: obtaining cell space group data to be processed, the cell space group data comprising an initial expression profile and a space coordinate set; performing feature extraction processing on the initial expression spectrum and the space coordinate set to obtain splicing features; performing cell type prediction processing based on the splicing features to obtain a cell type prediction result; the cell type prediction result is subjected to feature dimension splitting, a cell type proportion set and an expression profile weight set are obtained, and the expression profile weight set comprises sub-weight values of multiple features corresponding to each cell type in the cell type proportion set; and determining a cell type expression profile based on the initial expression profile and the expression profile weight set. According to the application, the accuracy of determining the proportion and type of the cells in the cell tissue can be improved.

The utility model provides a stem cell filtering and separating device. The stem cell filtering and separating device comprises a bottom cylinder and a cylinder cover, a filter cylinder is arranged in the bottom cylinder; filter paper is detachably placed on the inner side of the filter cartridge, the cartridge bottom of the filter cartridge is communicated with the top of the liquid storage cartridge, and a liquid discharge pipe is arranged at the bottom of the liquid storage cartridge; the liquid storage cylinder is connected with the upper part of the liquid storage connecting rod and is slidably connected with the bottom cylinder through the liquid storage connecting rod; the lower part of the liquid storage connecting rod is movably connected with one end of the lever plate in the length direction; the lever plate is hinged to the bottom cylinder, and the other end of the lever plate movably penetrates through the bottom cylinder and is movably connected with the bottom of the driving rod on the outer side of the bottom cylinder. One end of the cylinder cover is hinged to the bottom cylinder, the hinged position is arranged at the end where the driving rod is located, and the other end of the cylinder cover is detachably connected with the bottom cylinder. An annular water pipe is arranged on the inner side of the cylinder cover, and multiple nozzles are arranged on the inner side of the annular water pipe at intervals. According to the device, by changing the placing structure of the filter paper, an operator can better replace the filter paper and stem cell tissues, and the stem cell tissues can be protected while the stem cell filtering and separating effect is improved.

The present invention relates to specific natural or artificial RNA or DNA / RNA substrates for cleaving by a Cas nuclease. The invention furthermore relates to a complex comprising the specific artificial RNA or DNA / RNA substrate, at least one of a Cas nuclease enzyme and at least one preselected guide RNA binding to at least one target RNA. The present invention also relates to methods for cleaving the natural or artificial RNA or DNA / RNA substrate and methods for detecting at least one target RNA in a cell, tissue, cellular nucleus, and / or sample using the substrate or for eliminating a cell expressing the at least one target RNA.

The utility model relates to a portable embryo transfer device, aims at solving the technical problem that the current embryo transfer device is used in the process, through Pasteur pipette to hollow capsule is pressed, embryo is extracted and is transferred, and the precision of the staff is not good at the squeezing degree of the empty capsule, and it is often possible to be too violent and the cell tissue and liquid are sucked into the pipe body together, thereby influence the final sampling result, including the pipe body, its characterized in that, both ends of the pipe body are open setting, the pipe body is transparent pipe, one end of the pipe body is connected with the pipe head, the pipe body is connected with the elastic empty capsule away from the pipe head one end, the inner chamber sliding connection of pipe body has the piston, the fixed disc is fixedly installed in the pipe body above the piston, and the fixed disc is connected with the spring between the piston, the utility model has the accurate control elastic empty capsule squeezing degree, and then avoids the cell tissue and liquid to be sucked into the pipe body together and influence the final sampling result.

The invention relates to modified apolipoprotein with a targeting body. The targeting body may for example be an antibody or antigen binding fragment that allows targeting of e.g. a specific cell, tissue or organ. The modified apolipoprotein can be used as a carrier for a payload as such or when incorporated in a lipid nanoparticle. The modified apolipoprotein finds use in the treatment or prevention of diseases, or targeting a pay load to a specific target site.

A biological system for generating and preserving a repository of personalized, humanized transplantable cells, tissues, and organs for transplantation, wherein the biological system is biologically active and metabolically active, the biological system having genetically reprogrammed cells, tissues, and organs in a non-human animal for transplantation into a human recipient, wherein the non-human animal does not present one or more surface glycan epitopes and specific sequences from the wild-type swine's SLA is replaced with a synthetic nucleotides based on a human captured reference sequence from a human recipient's HLA.

The invention relates to a baculoviral vector system for improved delivery of DNA, RNA and protein into mammalian cells, including primary cells, tissues, and whole mammalian organisms.

The disclosure relates to compositions and methods for enhancing asymmetric division of satellite cells and promoting muscle cell / tissue regeneration, for treatment of muscle tissue injuries and muscle diseases, including muscular dystrophies.

There are provided herein, inter alia, complexes, compositions and methods for the delivery of therapeutic, diagnostic and imaging agents, including nucleic acid, into a cell. The complexes, compositions and methods may facilitate complexation, protection, delivery and release of oligonucleotides and polyanionic cargos into target cells, tissues, and organs both in vitro and in vivo.

The invention discloses a tissue separator for child operating room nursing, which comprises a refining rolling structure, a test tube clamping assembly, a clamping base, a main body bracket, a supporting top plate and a rolling assembly supporting arm, and belongs to the technical field of tissue separation. On one hand, the pressure of the cell tissues between the fixing disc and the nylon net can be increased, and on the other hand, the contact area of the cell tissues and the nylon net can be increased, so that the cell tissues form a cell suspension more quickly, and the cell suspension enters the cell suspension storage box through the nylon net to be stored; and the cell suspension can be discharged through the valve on the liquid outlet at the bottom, so that the cell suspension enters the test tube below.

The invention belongs to the technical field of biomedical engineering and microfluidics, and particularly discloses a double-channel vascularization organ chip which comprises a channel layer and a bottom layer which are arranged in a stacked mode, and the lower surface of the channel layer and the bottom layer are sealed through bonding; a culture solution channel is arranged on the lower surface of the runner layer, snakelike pipelines are symmetrically arranged on the culture solution channel, and a first liquid injection channel and a second liquid injection channel are arranged at the two ends of the culture solution channel respectively; the lower surface of the runner layer is provided with a first cell culture chamber and a second cell culture chamber which are communicated with the culture solution channel; by adopting a double-layer structure of the flow channel layer and the bottom layer, the micro-fluidic chip has the characteristics of simple structure, convenience in operation, easiness in manufacturing of the chip and the like, and the first cell culture chamber and the second cell culture chamber are separated by the second micro-column; the interaction among different types of cell tissues in the first cell culture chamber and the second cell culture chamber can be realized by utilizing the gaps among the second micro-columns, so that the complex interaction among multiple tissues in the body can be really simulated.

Macromolecule compositions and related methods that effect targeted delivery of therapeutic agents to effector targets in a desired cell, tissue and / or organ of interest while minimizing or avoiding undesirable delivery to other cells, tissues or organs are provided. Compositions and methods related to macromolecules, such as an ANDbody™, that include an effector target binding domain specific for an effector target, and an address binding domain specific for an address target are described. The macromolecules are linked to small molecules.

BANF1, PPP2CA, and ANKLE2 were identified as genes that promote tau aggregation when disrupted. Improved tauopathy models such as cells, tissues, or animals having mutations in or inhibition of expression of BANF1 and / or PPP2CA and / or ANKLE2 are provided. Methods of using such improved tauopathy models for assessing therapeutic candidates for the treatment of a tauopathy, methods of making the improved tauopathy models, and methods of accelerating or exacerbating tau aggregation in a tauopathy model are also provided.

Aspects of the present invention include methods and compositions related to the production and use of pluripotent stem cell-derived clonal embryonic progenitor cell types useful in the generation of cellular components of brown adipocyte tissue for research and therapy relating to applications in obesity, diabetes, and cardiovascular disease.

The invention discloses culture equipment for biological cell tissues, and relates to the technical field of biological cell culture. The device comprises a culture box and a rotating shaft, the rotating shaft is rotatably installed in the culture box, a box door is fixedly installed on the rotating shaft, a transmission mechanism is arranged on the rotating shaft, the transmission mechanism is meshed with a rack, a connecting rod is fixedly installed on the rack, the connecting rod is tightly attached to an air rod, the air rod is slidably connected into an air cylinder, and an air guide pipe is arranged at the front end of the air cylinder. A one-way control valve is installed at the front end of the air guide pipe, the front end of the air guide pipe is communicated with a disinfection water tank, the front side of the disinfection water tank is communicated with a pressure valve pipe, the lower end of the pressure valve pipe is communicated with a splitter plate, and a rotating shaft is arranged in the incubator. By opening the box door, the splitter plate can automatically spray vaporific disinfectant fluid downwards, and the vaporific disinfectant fluid can form an atomized protective layer of the disinfectant fluid, so that bacteria or impurities in the external environment are prevented from entering the incubator after the box door is opened.

The utility model relates to the technical field of cell tissue culture, and discloses automatic liquid adding equipment for cell tissue culture, which comprises a cell culture chamber, an operation panel, a culture chamber main chamber, a chamber door, a moving component, a liquid suction device, a solution bottle, a support plate and a culture dish, the cell culture chamber is hermetically fixed at the right part of the upper end of the supporting plate, the operation panel is obliquely arranged at the upper part of the culture chamber main chamber, the chamber door is arranged on the front side of the cell culture chamber in an openable manner, and the chamber door and the cell culture chamber are kept sealed; the moving assembly is fixed to the right side of the upper portion of the culture room main chamber, the liquid suction device is fixed to the driving end of the moving assembly, multiple rows of culture dishes and multiple solution bottles are arranged at the upper end of the supporting plate, and reagents needing to be added are stored in the solution bottles. The cell tissue culture device can guarantee the stability of a culture environment, improve the culture quality of cell tissues, reduce the working intensity of testers and facilitate flexible selection of culture schemes.

The article is a tool that assists in collecting a sample (e.g., cells, tissues, or fluids (e.g., blood) from a living organism (e.g., human body, animal, plant, etc.)). The article comprises a main body and a holder. As shown in the exploded view, the holder is configured to be attached to the main body via a connecting member by inserting one end of the connecting member into the holder while inserting the other end of the connecting member into the main body. The holder has an abutting portion that abuts against the syringe of the syringe and a fitting portion that fits into the reduced diameter portion of the piston rod of the syringe. The fitting portion of the holder is configured to be displaceable between a first position as shown in the top perspective view or bottom perspective view, and a second position as shown in the top perspective view or bottom perspective view showing a changed state. With the syringe of the syringe abutting the abutment portion of the holder and the reduced diameter portion of the piston rod of the syringe engaged with the engaging portion of the holder, the user can grasp the main body of the item and displace the engaging portion of the item with their fingers from a first position to a second position, thereby creating a negative pressure inside the syringe of the syringe and using the syringe to stably and accurately collect a predetermined amount of sample.

The production method includes: linearly printing a plurality of cells between a first substrate and a second substrate in such a manner that both ends of the plurality of cells are supported on the first substrate and the second substrate, respectively; obtaining a plurality of cell tissues T by culturing the printed plurality of cells; filling a soluble liquid holding material between the first substrate and the second substrate; curing the filled holding material; separating each of the plurality of cell tissues T from at least one base material; and obtaining a plurality of blocks 50C by dividing the holding material along an extending direction of the plurality of cell tissues T held by the cured holding material. The obtaining of the plurality of blocks 50C includes housing the cured holding material in a dividing housing 30 provided with a plurality of blades 31.

The utility model relates to the technical field of cell culture, and discloses a cell shake flask capable of improving tissue digestion efficiency, which comprises a culture flask body, a flask cover is mounted at the top end of the culture flask body, and a first constant-temperature heating cover is arranged on one side of the outer side wall of the culture flask body. Mounting blocks are fixed to the front end and the rear end of one side of the first constant-temperature heating cover correspondingly, inner cavities are formed in the first constant-temperature heating cover and the second constant-temperature heating cover correspondingly, and electric heating wires are mounted in the inner cavities. When the culture bottle body disclosed by the utility model is used for culturing cells, the culture bottle body can be heated according to actual conditions by arranging components such as the first constant-temperature heating cover, the mounting block, the mounting groove and the second constant-temperature heating cover, and meanwhile, the digestion efficiency of cell tissues can be promoted by heating the culture bottle body; and the culture bottle body can be detached when not needed subsequently, so that the observation of the condition of cells in the culture bottle body through the culture bottle body is not influenced, and the use effect is better.

Provided are methods for rapid multiplex HLA genotyping and transcript quantitation. In some embodiments, the presently disclosed methods include providing a. sample from the cell, tissue, or organ that has mRNA derived from an HLA gene product; reverse transcribing the mRNA to produce a. pool of cDNAs; amplifying the cDNAs that result from reverse transcription of HLA-specific mRNAs to produce a pool of HLA-specific cDNAs; and determining the sequence of each HLA-specific cDNAs, whereby the HLA status of the cell, tissue, organ, or subject is determined. Also provided are methods for genotyping donor cells, tissues, and / or organs meant for transplantation into recipients. In some embodiments, the methods can be used to identify the presence of absence of each of HLA-A, -B, -C, -DRB 1 / 3 / 4 / 5, -DQA1, and -DQB1 in a biological sample.