152 results about "Cell organisation" patented technology

The invention discloses a preparation method of DNA (Deoxyribonucleic Acid) hydrogel with programmable mechanical strength. By regulating and controlling the molar ratio of the catalytic initiating chains to the total amount of the DNA monomer building blocks, multiple hydrogels which are equivalent in mass concentration and have different mechanical strengths are constructed. According to the method, a strategy similar to catalytic assembly is adopted, the feeding molar ratio of a catalytic initiating chain to a DNA monomer building block is creatively adjusted, the proportion of cross-linking reactions at different levels is regulated and controlled, then the DNA hydrogel with different mechanical strengths is synthesized under the isothermal condition, and the storage modulus range is about 200-2000 Pa. In addition, the change of the mechanical strength is independent of the total concentration of the matrix (DNA) of the hydrogel, the DNA sequence, the stress relaxation rate and other properties. The method provides a novel convenient method for constructing DNA hydrogels with different mechanical strengths, and possibly provides a novel tool for researching the specific influence of mechanical properties of materials on cell / tissue behaviors.

Methods and compositions for modulating a target genome are disclosed. This disclosure relates to novel compositions, systems and methods for altering a genome at one or more locations in a host cell, tissue or subject, in vivo or in vitro. In particular, the invention features compositions, systems and methods for inserting, altering, or deleting sequences of interest in a host genome.

Compositions, methods and systems for targeted delivery using one or more shuttles operably linked to one or more cargos (e.g., cargo polypeptides, e.g., cargo oligonucleotides) and subsequent quantification of an abundance of one or more cargos (e.g, proteins) in a mixture (e.g., a complex mixture (e.g., in vivo)) or in a specific cell, tissue, or organ of interest (e.g., in vivo) using barcodes (e.g., peptide barcodes), binders (e.g., polypeptide binders), and binding agents (e.g., phage) are provided herein.

The invention discloses a large model and multi-mode fused lysine modification site prediction method and device, relates to the technical field of biological information, and mainly aims to solve the problem of poor prediction effectiveness of lysine modification sites in existing proteins. Comprising the following steps: acquiring amino acid sequence information of cell tissue protein, and processing the amino acid sequence information based on a protein large language model which completes model training to obtain sequence representation; obtaining amino acid atom information of the cell tissue protein, and processing an amino acid contact graph structure constructed based on the amino acid atom coordinate information based on a graph structure model of which model training is completed to obtain structural characterization; and splicing the sequence representation and the structure representation, and performing feature fusion prediction on a representation vector obtained after splicing based on a multi-modal fusion model of which model training is completed to obtain a lysine modification site prediction result.

The invention relates to the technical field of culture devices, and discloses a fan regulation and control method for an incubator, which comprises the following steps: acquiring a temperature rise stage and an inner chamber temperature of the incubator and an environment temperature of an environment where the incubator is located; under the condition that the inner chamber temperature does not meet the first inner chamber temperature condition, according to the temperature rising stage of the incubator, a circulating fan is controlled to operate according to the temperature rising air speed; and after the inner chamber temperature meets the first inner chamber temperature condition, the temperature rising air speed of the circulating fan is adjusted according to the environment temperature. According to the method, the problem of back drop after temperature overshoot after the incubator is heated for the first time can be solved, the temperature fluctuation range of the temperature in the inner chamber is controlled, and the culture effect of cells / tissues in the incubator is improved. The invention further discloses a fan regulation and control device for the incubator, the incubator and a storage medium.

The invention provides a cell data processing method and device, equipment, a storage medium and a program product. The method comprises: obtaining cell space group data to be processed, the cell space group data comprising an initial expression profile and a space coordinate set; performing feature extraction processing on the initial expression spectrum and the space coordinate set to obtain splicing features; performing cell type prediction processing based on the splicing features to obtain a cell type prediction result; the cell type prediction result is subjected to feature dimension splitting, a cell type proportion set and an expression profile weight set are obtained, and the expression profile weight set comprises sub-weight values of multiple features corresponding to each cell type in the cell type proportion set; and determining a cell type expression profile based on the initial expression profile and the expression profile weight set. According to the application, the accuracy of determining the proportion and type of the cells in the cell tissue can be improved.

The present invention relates to specific natural or artificial RNA or DNA / RNA substrates for cleaving by a Cas nuclease. The invention furthermore relates to a complex comprising the specific artificial RNA or DNA / RNA substrate, at least one of a Cas nuclease enzyme and at least one preselected guide RNA binding to at least one target RNA. The present invention also relates to methods for cleaving the natural or artificial RNA or DNA / RNA substrate and methods for detecting at least one target RNA in a cell, tissue, cellular nucleus, and / or sample using the substrate or for eliminating a cell expressing the at least one target RNA.

The utility model relates to a portable embryo transfer device, aims at solving the technical problem that the current embryo transfer device is used in the process, through Pasteur pipette to hollow capsule is pressed, embryo is extracted and is transferred, and the precision of the staff is not good at the squeezing degree of the empty capsule, and it is often possible to be too violent and the cell tissue and liquid are sucked into the pipe body together, thereby influence the final sampling result, including the pipe body, its characterized in that, both ends of the pipe body are open setting, the pipe body is transparent pipe, one end of the pipe body is connected with the pipe head, the pipe body is connected with the elastic empty capsule away from the pipe head one end, the inner chamber sliding connection of pipe body has the piston, the fixed disc is fixedly installed in the pipe body above the piston, and the fixed disc is connected with the spring between the piston, the utility model has the accurate control elastic empty capsule squeezing degree, and then avoids the cell tissue and liquid to be sucked into the pipe body together and influence the final sampling result.

The invention relates to modified apolipoprotein with a targeting body. The targeting body may for example be an antibody or antigen binding fragment that allows targeting of e.g. a specific cell, tissue or organ. The modified apolipoprotein can be used as a carrier for a payload as such or when incorporated in a lipid nanoparticle. The modified apolipoprotein finds use in the treatment or prevention of diseases, or targeting a pay load to a specific target site.

A biological system for generating and preserving a repository of personalized, humanized transplantable cells, tissues, and organs for transplantation, wherein the biological system is biologically active and metabolically active, the biological system having genetically reprogrammed cells, tissues, and organs in a non-human animal for transplantation into a human recipient, wherein the non-human animal does not present one or more surface glycan epitopes and specific sequences from the wild-type swine's SLA is replaced with a synthetic nucleotides based on a human captured reference sequence from a human recipient's HLA.

The disclosure relates to compositions and methods for enhancing asymmetric division of satellite cells and promoting muscle cell / tissue regeneration, for treatment of muscle tissue injuries and muscle diseases, including muscular dystrophies.

There are provided herein, inter alia, complexes, compositions and methods for the delivery of therapeutic, diagnostic and imaging agents, including nucleic acid, into a cell. The complexes, compositions and methods may facilitate complexation, protection, delivery and release of oligonucleotides and polyanionic cargos into target cells, tissues, and organs both in vitro and in vivo.

The invention discloses a tissue separator for child operating room nursing, which comprises a refining rolling structure, a test tube clamping assembly, a clamping base, a main body bracket, a supporting top plate and a rolling assembly supporting arm, and belongs to the technical field of tissue separation. On one hand, the pressure of the cell tissues between the fixing disc and the nylon net can be increased, and on the other hand, the contact area of the cell tissues and the nylon net can be increased, so that the cell tissues form a cell suspension more quickly, and the cell suspension enters the cell suspension storage box through the nylon net to be stored; and the cell suspension can be discharged through the valve on the liquid outlet at the bottom, so that the cell suspension enters the test tube below.

The invention belongs to the technical field of biomedical engineering and microfluidics, and particularly discloses a double-channel vascularization organ chip which comprises a channel layer and a bottom layer which are arranged in a stacked mode, and the lower surface of the channel layer and the bottom layer are sealed through bonding; a culture solution channel is arranged on the lower surface of the runner layer, snakelike pipelines are symmetrically arranged on the culture solution channel, and a first liquid injection channel and a second liquid injection channel are arranged at the two ends of the culture solution channel respectively; the lower surface of the runner layer is provided with a first cell culture chamber and a second cell culture chamber which are communicated with the culture solution channel; by adopting a double-layer structure of the flow channel layer and the bottom layer, the micro-fluidic chip has the characteristics of simple structure, convenience in operation, easiness in manufacturing of the chip and the like, and the first cell culture chamber and the second cell culture chamber are separated by the second micro-column; the interaction among different types of cell tissues in the first cell culture chamber and the second cell culture chamber can be realized by utilizing the gaps among the second micro-columns, so that the complex interaction among multiple tissues in the body can be really simulated.

Macromolecule compositions and related methods that effect targeted delivery of therapeutic agents to effector targets in a desired cell, tissue and / or organ of interest while minimizing or avoiding undesirable delivery to other cells, tissues or organs are provided. Compositions and methods related to macromolecules, such as an ANDbody™, that include an effector target binding domain specific for an effector target, and an address binding domain specific for an address target are described. The macromolecules are linked to small molecules.

The utility model relates to the technical field of cell tissue culture, and discloses automatic liquid adding equipment for cell tissue culture, which comprises a cell culture chamber, an operation panel, a culture chamber main chamber, a chamber door, a moving component, a liquid suction device, a solution bottle, a support plate and a culture dish, the cell culture chamber is hermetically fixed at the right part of the upper end of the supporting plate, the operation panel is obliquely arranged at the upper part of the culture chamber main chamber, the chamber door is arranged on the front side of the cell culture chamber in an openable manner, and the chamber door and the cell culture chamber are kept sealed; the moving assembly is fixed to the right side of the upper portion of the culture room main chamber, the liquid suction device is fixed to the driving end of the moving assembly, multiple rows of culture dishes and multiple solution bottles are arranged at the upper end of the supporting plate, and reagents needing to be added are stored in the solution bottles. The cell tissue culture device can guarantee the stability of a culture environment, improve the culture quality of cell tissues, reduce the working intensity of testers and facilitate flexible selection of culture schemes.

The production method includes: linearly printing a plurality of cells between a first substrate and a second substrate in such a manner that both ends of the plurality of cells are supported on the first substrate and the second substrate, respectively; obtaining a plurality of cell tissues T by culturing the printed plurality of cells; filling a soluble liquid holding material between the first substrate and the second substrate; curing the filled holding material; separating each of the plurality of cell tissues T from at least one base material; and obtaining a plurality of blocks 50C by dividing the holding material along an extending direction of the plurality of cell tissues T held by the cured holding material. The obtaining of the plurality of blocks 50C includes housing the cured holding material in a dividing housing 30 provided with a plurality of blades 31.

The utility model relates to the technical field of cell culture, and discloses a cell shake flask capable of improving tissue digestion efficiency, which comprises a culture flask body, a flask cover is mounted at the top end of the culture flask body, and a first constant-temperature heating cover is arranged on one side of the outer side wall of the culture flask body. Mounting blocks are fixed to the front end and the rear end of one side of the first constant-temperature heating cover correspondingly, inner cavities are formed in the first constant-temperature heating cover and the second constant-temperature heating cover correspondingly, and electric heating wires are mounted in the inner cavities. When the culture bottle body disclosed by the utility model is used for culturing cells, the culture bottle body can be heated according to actual conditions by arranging components such as the first constant-temperature heating cover, the mounting block, the mounting groove and the second constant-temperature heating cover, and meanwhile, the digestion efficiency of cell tissues can be promoted by heating the culture bottle body; and the culture bottle body can be detached when not needed subsequently, so that the observation of the condition of cells in the culture bottle body through the culture bottle body is not influenced, and the use effect is better.

Provided are methods for rapid multiplex HLA genotyping and transcript quantitation. In some embodiments, the presently disclosed methods include providing a. sample from the cell, tissue, or organ that has mRNA derived from an HLA gene product; reverse transcribing the mRNA to produce a. pool of cDNAs; amplifying the cDNAs that result from reverse transcription of HLA-specific mRNAs to produce a pool of HLA-specific cDNAs; and determining the sequence of each HLA-specific cDNAs, whereby the HLA status of the cell, tissue, organ, or subject is determined. Also provided are methods for genotyping donor cells, tissues, and / or organs meant for transplantation into recipients. In some embodiments, the methods can be used to identify the presence of absence of each of HLA-A, -B, -C, -DRB 1 / 3 / 4 / 5, -DQA1, and -DQB1 in a biological sample.

The invention relates to the technical field of cell tissue sampling, and discloses a cancer tissue slice sampling device for tumor immunoassay, the cancer tissue slice sampling device comprises a case, a wax coating constraint assembly, a slice shifting assembly, a slice linear driver, a slice cutter, a feeding linear motor and a lifting bracket, and the wax coating constraint assembly is located in the case. The invention particularly provides a cancer tissue slice sampling device for tumor immunoassay, which can stably position a wax coated sample and can take slices one by one after integral cutting.

The present disclosure provides methods for inducing cellular quiescence—defined as a reversible arrest of cell proliferation—by inhibiting ribosome biogenesis. The methods involve administering a quiescence-inducing agent, such as a small molecule inhibitor, peptide, antisense oligonucleotide, or targeted degrader, that interferes with rRNA transcription, processing, ribosomal protein synthesis, or ribosome assembly. The agent may be applied to cultured cells, tissues, organs, or organoids, across a range of cell types and species. In some embodiments, the methods reduce metabolic activity and enhance stress resistance in non-dividing or post-mitotic cells. By slowing proliferation and / or metabolism while preserving viability, these methods enable improved preservation of cells, tissues, and organs-enhancing transplantation logistics, biobanking, regenerative medicine workflows, and biological transport. Additional applications include studies of cell cycle regulation, tissue regeneration, stress biology, aging, and induction of organismal hypometabolism (e.g., torpor or hibernation). The disclosure includes examples demonstrating quiescence induction using chemical biology systems, pharmacological agents, and genetic approaches.

The production method includes: filling a soluble liquid holding material that cures under a predetermined condition between a first substrate and a second substrate; curing the filled holding material; and separating at least one cell tissue from at least one of the first substrate and the second substrate by cutting out the cured holding material from the first substrate and the second substrate.

The present application relates to a kind of new cell penetrating peptides and its use. It is proved that the 12 cell penetrating peptides of the present application have excellent cell penetration ability and mass transfer effect in vitro and in vivo, since the above-mentioned cell penetrating peptides can effectively deliver biologically active substances to cells, tissues and other in vivo, and are expected to be widely used in research fields, various disease diagnosis and treatment fields, etc.

The present disclosure is in the field of culturing a homocellular cell culture of progenitor cells form a heterocellular tissue sample. The present disclosure relates to a method for specifically generating muscle progenitor cells or adipogenic progenitor cells from an isolated tissue sample, preferably a muscle tissue sample, e.g., from mammalian origin, preferably from bovine, ovine, murine, or porcine origin. The method includes culturing a heterocellular tissue sample comprising a muscle progenitor cell and an adipogenic progenitor cell in a selection medium including comprsing at least one compound which specifically promotes the proliferation of muscle progenitor cells or of adipogenic progenitor cells leading to a homocellular cell culture. Further, the present disclosure relates to the selection medium which facilitates selective generation of muscle progenitor cells or of adipogenic progenitor cells. The method and selection medium of the invention can be used for producing homocellular cell cultures of muscle progenitor cells or of adipogenic progenitor cells. The homocellular cell cultures produced according to the present disclosure can be used to produce a cell culture-based meat product, preferably for animal or human consumption.

This disclosure provides compositions, systems, and methods for targeting, editing, modifying, or manipulating the genome of a host cell at one or more locations in a DNA sequence within a cell, tissue, or object. A genetically modified system for treating phenylketonuria (PKU) is described. In one embodiment, this disclosure relates to a pharmaceutical composition comprising the above system and a pharmaceutically acceptable excipient or carrier, wherein the pharmaceutically acceptable excipient or carrier is selected from the group consisting of plasmid vectors, viral vectors, vesicles, and lipid nanoparticles.

The invention relates to the technical field of biology, in particular to a biomechanical system for cell tissue culture and biological material preparation, which comprises a reactor module, a power pressurization module, a monitoring sensor, a computer and a circulation module, the reactor module is arranged on the power pressurization module, the monitoring sensor is connected with the power pressurization module, and the computer is connected with the circulation module. The computer is connected with the monitoring sensor; the circulating module is connected with the sensor and the reactor module; the power pressurization module comprises a rack, a driving assembly, a screw and a pressure sensor, the screw and the driving assembly are installed on the rack, the driving assembly drives the pressure sensor to move through the screw, and in this way, the problems that in the prior art, a biomechanical device is too large in size, too heavy in mass, high in use cost and poor in practicability due to equipment integration are solved. The mechanical control precision is poor, and the pressurizing effect is single.

The invention discloses a biological sample whole RNA normal-temperature long-term preservative as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The preservative comprises the following components: a tissue fixing agent, a tissue penetrating agent, a reactive biological sample encapsulating agent, a silicification blocking agent, a pH regulator, an ion concentration regulator and water. The preservative can be applied to bacteria, such as escherichia coli and staphylococcus aureus; a cell, such as an HEK293 cell, an ID8 cell; tissues such as mouse liver tissues and human tumor tissues; organs such as kidneys and hearts of small animals; the whole RNA of a complete organism, such as earthworms, fruit flies and other biological samples with different sizes can be stored at normal temperature for a long time, so that the RNA extraction efficiency and quality can be ensured, the method is particularly suitable for normal-temperature transfer and long-term storage of field collected samples and clinical submitted samples, and the RNA extraction efficiency and quality in subsequent molecular experiments can be ensured; the method is of great significance to molecular biology research and clinical diagnosis.

The invention relates to a cell tissue culture device special for primary cells, and relates to the technical field of cell culture, the cell tissue culture device comprises an incubator, the interior of the incubator is divided into three independent culture chambers through partition plates, each culture chamber is internally provided with at least three culture dishes, and the cell tissue culture device further comprises a dynamic culture assembly, at least two culture rooms are provided with a dynamic culture assembly, the dynamic culture assembly is provided with a micro-channel structure capable of simulating blood vessels, the dynamic culture assembly, the electrophysiological stimulation piece and the traction force simulation piece are integrated to form the culture system capable of simulating various physiological conditions in the body, the dynamic culture assembly simulates a blood vessel microchannel to realize low-shear-force continuous perfusion; the electrophysiological stimulation piece simulates a neuroelectric signal; the traction force simulation part applies controllable mechanical traction force through a multi-caliber breather pipe, and the three parts have a synergistic effect, so that the physiological function expression and culture quality of the primary cells are improved.

Methods and compositions for modulating a target genome are disclosed. This disclosure relates to novel compositions, systems and methods for altering a genome at one or more locations in a host cell, tissue or subject, in vivo or in vitro. In particular, the invention features compositions, systems and methods for inserting, altering, or deleting sequences of interest in a host genome.