325 results about "Cell organisation" patented technology

The present invention, among other things, provides technologies for detecting and / or quantifying nucleic acids in cells, tissues, organs, or organisms. Pre-designed barcodes are associated with specific molecular targets through sequential hybridization experiments. A pseudo-color based barcoding scheme is described that overcomes the limitations in the previous generation of the technology. The current method can be applied to both in vitro and in situ analysis.

The invention discloses a preparation method of DNA (Deoxyribonucleic Acid) hydrogel with programmable mechanical strength. By regulating and controlling the molar ratio of the catalytic initiating chains to the total amount of the DNA monomer building blocks, multiple hydrogels which are equivalent in mass concentration and have different mechanical strengths are constructed. According to the method, a strategy similar to catalytic assembly is adopted, the feeding molar ratio of a catalytic initiating chain to a DNA monomer building block is creatively adjusted, the proportion of cross-linking reactions at different levels is regulated and controlled, then the DNA hydrogel with different mechanical strengths is synthesized under the isothermal condition, and the storage modulus range is about 200-2000 Pa. In addition, the change of the mechanical strength is independent of the total concentration of the matrix (DNA) of the hydrogel, the DNA sequence, the stress relaxation rate and other properties. The method provides a novel convenient method for constructing DNA hydrogels with different mechanical strengths, and possibly provides a novel tool for researching the specific influence of mechanical properties of materials on cell / tissue behaviors.

This disclosure describes methods for growing cells on chickpea microcarriers as part of a process of creating a comestible food product. Generally, the disclosed method comprises adding chickpea microcarriers to cell culture media in a vessel. The chickpea microcarriers are mixed with non-human cells in the cell culture media. The chickpea microcarriers and non-human cells are mixed to adhere and grow the non-human cells on the surface of the chickpea microcarriers. After mixing, the non-human cells adhered to the surface of the chickpea microcarriers forms a textured cell tissue. The textured cell tissue can be harvested from the vessel and formed into a comestible food product.

The invention provides an organ chip unit for simulating the pathogenesis of rheumatoid arthritis, and belongs to the technical field of cell culture. The chip comprises a top-layer chip, a bottom-layer chip and a middle microporous membrane, the top-layer chip and the bottom-layer chip are separated by the middle microporous membrane, and the middle microporous membrane is used for enabling cells in a cell culture space of the top-layer chip and cells in a cell culture space of the bottom-layer chip not to be in direct contact, but interaction of signal molecules and substance exchange can be carried out; synovial cells and cartilage cells are injected into the bottom-layer chip or the top-layer chip to be cultured. According to the invention, multi-cell three-dimensional co-culture can be realized. Through the additional stress stimulation co-culture part, the influence of pressure or tension on the cell environment can be judged, and the actual condition that rheumatoid arthritis joints receive pressure or tension for a long time can be simulated. The synovial cells and the cartilage cells are used, so that the structure of the inflammation joint in a living body can be truly reflected, and the interaction among various cell tissues can be reflected.

The utility model relates to the technical field of biological tissue engineering, in particular to an organ chip platform integrated with optical tweezers, which is used for assembling and culturing three-dimensional cell tissues and comprises an organ chip with optical transparency and holographic optical tweezers integrated with the organ chip. The organ chip is horizontally arranged and comprises an upper-layer plate, a middle-layer plate and a lower-layer plate which are sequentially stacked, the middle-layer plate is provided with at least two first culture pools used for inducing and differentiating stem cells into different types of cells and at least one second culture pool used for assembling and culturing the different types of cells, and the lower-layer plate is provided with at least two second culture pools used for assembling and culturing the different types of cells. The channel is connected with the first culture pond and the second culture pond; and an objective lens of the holographic optical tweezers faces the top surface or the bottom surface of the organ chip and is used for capturing, moving and assembling cells in the first culture pool or the second culture pool. According to the utility model, the dependence on a micro-pattern processing technology can be reduced, the cost is reduced, and the regulation and control capability on an organoid tissue structure is improved.

The invention relates to a mechanical compression and / or co-culture microfluid device and application thereof, and belongs to the technical field of biomedical science or pharmacology, the microfluid device comprises a culture chamber and a nutrition supply chamber, the culture chamber comprises at least two compartments for accommodating cells / tissues and a porous limiting plate shared with the nutrition supply chamber, the compartments at least comprise a compression compartment for accommodating cells / tissues subjected to mechanical compression and a non-compression compartment which is not subjected to compression strength or is subjected to compression strength far lower than the compression strength of the compression compartment, and the actuating chamber drives the flexible partition plate on the bottom surface of the culture chamber to periodically deform and restore to the original shape after being in contact with the limiting plate; and at least one compartment of the nutrition supply room corresponds to the culture room. The device is free of complex alignment operation, low in manufacturing difficulty, low in cost and particularly suitable for simulating the microenvironment of cartilage tissue, the thin face shape formed by the compression compartment simulates the shape and nutrition supply mode of cartilage, and meanwhile the non-compression compartment can achieve co-culture of multiple kinds of non-compression tissue and compressed cartilage tissue.

The invention discloses a dual-recognition-framework nucleic acid probe, a preparation method and application, the dual-recognition-framework nucleic acid probe comprises a DNA tetrahedron, and a dual-recognition module and a fuel amplification module which are connected to the vertex of the DNA tetrahedron through complementary pairing of bases, ATP recognition protection and dual-target response are introduced, and the dual-recognition-framework nucleic acid probe is obtained through sequential opening of internal and external locks. The occurrence of false positive conditions can be reduced, and the specificity is effectively improved; signal amplification is realized by virtue of abnormal APE1 expression of tumor cells, so that a fluorescence signal for miRNA detection is increased, and the sensitivity and the imaging signal-to-noise ratio are effectively improved; the preparation is easy; the use is convenient; complicated equipment is not needed. The method can be applied to a target nucleic acid detection reagent or an imaging or positioning reagent of cells, tissues or organs containing target nucleic acid.

Methods and compositions for modulating a target genome are disclosed. This disclosure relates to novel compositions, systems and methods for altering a genome at one or more locations in a host cell, tissue or subject, in vivo or in vitro. In particular, the invention features compositions, systems and methods for inserting, altering, or deleting sequences of interest in a host genome.

Compositions, methods and systems for targeted delivery using one or more shuttles operably linked to one or more cargos (e.g., cargo polypeptides, e.g., cargo oligonucleotides) and subsequent quantification of an abundance of one or more cargos (e.g, proteins) in a mixture (e.g., a complex mixture (e.g., in vivo)) or in a specific cell, tissue, or organ of interest (e.g., in vivo) using barcodes (e.g., peptide barcodes), binders (e.g., polypeptide binders), and binding agents (e.g., phage) are provided herein.

The invention discloses a large model and multi-mode fused lysine modification site prediction method and device, relates to the technical field of biological information, and mainly aims to solve the problem of poor prediction effectiveness of lysine modification sites in existing proteins. Comprising the following steps: acquiring amino acid sequence information of cell tissue protein, and processing the amino acid sequence information based on a protein large language model which completes model training to obtain sequence representation; obtaining amino acid atom information of the cell tissue protein, and processing an amino acid contact graph structure constructed based on the amino acid atom coordinate information based on a graph structure model of which model training is completed to obtain structural characterization; and splicing the sequence representation and the structure representation, and performing feature fusion prediction on a representation vector obtained after splicing based on a multi-modal fusion model of which model training is completed to obtain a lysine modification site prediction result.

The invention relates to a pathological image spatial feature extraction method and an electronic device, and the method comprises the steps: respectively obtaining image block features and cell features from a region of interest, carrying out the spatial modeling of the image block features and the cell features, and respectively extracting tissue pathological features and cell pathological features based on a cell map and a tissue map; and fusing the two to obtain a final pathological feature. A cell map and a tissue map are respectively constructed from two perspectives of cells and tissues, the cell map properly contains distribution and interaction of the cells, the tissue map properly encodes a tissue microenvironment including tissue topology distribution which cannot be summarized by the cell map, and finally spatial information contained in the cell map and the tissue map is fused. Therefore, cell and tissue information in the pathological image is fully utilized, compared with a network which only uses a cell map or a tissue map, rich information in the pathological image can be better captured by simultaneously using the two types of maps, and the purpose of fully utilizing multi-scale space information in the pathological image can be achieved.

The invention relates to the technical field of culture devices, and discloses a fan regulation and control method for an incubator, which comprises the following steps: acquiring a temperature rise stage and an inner chamber temperature of the incubator and an environment temperature of an environment where the incubator is located; under the condition that the inner chamber temperature does not meet the first inner chamber temperature condition, according to the temperature rising stage of the incubator, a circulating fan is controlled to operate according to the temperature rising air speed; and after the inner chamber temperature meets the first inner chamber temperature condition, the temperature rising air speed of the circulating fan is adjusted according to the environment temperature. According to the method, the problem of back drop after temperature overshoot after the incubator is heated for the first time can be solved, the temperature fluctuation range of the temperature in the inner chamber is controlled, and the culture effect of cells / tissues in the incubator is improved. The invention further discloses a fan regulation and control device for the incubator, the incubator and a storage medium.

An ultra-high-cell-density multicellular tissue lifting bioprinting device assisted by oblique incident bulk wave and a printing method therefor are provided, and the printing device includes a printing liquid tank, a printing platform, a light source and an oblique incident bulk wave chip; the printing liquid tank contains a printing liquid which is a mixture of cells and a photocurable bioink; the printing platform is arranged horizontally and extends into the printing liquid tank to come into contact with the printing liquid; the printing platform is capable of going up and down; the light source is arranged under the printing liquid tank, and light enters the printing liquid tank; the oblique incident bulk wave chip is a piezoelectric transducer arranged obliquely; the piezoelectric transducer is arranged under the printing liquid tank and tilted relative to the horizontal plane to generate an oblique incident bulk wave acoustic field in the printing liquid.

The invention provides a cell data processing method and device, equipment, a storage medium and a program product. The method comprises: obtaining cell space group data to be processed, the cell space group data comprising an initial expression profile and a space coordinate set; performing feature extraction processing on the initial expression spectrum and the space coordinate set to obtain splicing features; performing cell type prediction processing based on the splicing features to obtain a cell type prediction result; the cell type prediction result is subjected to feature dimension splitting, a cell type proportion set and an expression profile weight set are obtained, and the expression profile weight set comprises sub-weight values of multiple features corresponding to each cell type in the cell type proportion set; and determining a cell type expression profile based on the initial expression profile and the expression profile weight set. According to the application, the accuracy of determining the proportion and type of the cells in the cell tissue can be improved.

The invention relates to the technical field of organ chips and tissue engineering, in particular to an organ chip platform integrated with optical tweezers and a three-dimensional cell assembling method, the platform comprises an organ chip with optical transparency and an optical tweezers system integrated with the organ chip; the organ chip comprises at least two induced differentiation culture pools and at least one assembly culture pool; the induced differentiation culture pool is used for respectively culturing the stem cells and inducing and differentiating the stem cells into at least two different types of cells; the optical tweezers system is used for capturing cells and moving the cells from the induced differentiation culture pool to the assembly culture pool; assembling the at least two different types of cells into an initial three-dimensional cell tissue according to the proportion, sequence and pattern structure in the target organ tissue in the assembly culture pool; the assembly culture pool is used for culturing the initial three-dimensional cell tissue to obtain the three-dimensional cell tissue with the target pattern structure. The technical threshold of the organ chip can be reduced, and the biological functionality of the organ chip is improved.

The utility model provides a stem cell filtering and separating device. The stem cell filtering and separating device comprises a bottom cylinder and a cylinder cover, a filter cylinder is arranged in the bottom cylinder; filter paper is detachably placed on the inner side of the filter cartridge, the cartridge bottom of the filter cartridge is communicated with the top of the liquid storage cartridge, and a liquid discharge pipe is arranged at the bottom of the liquid storage cartridge; the liquid storage cylinder is connected with the upper part of the liquid storage connecting rod and is slidably connected with the bottom cylinder through the liquid storage connecting rod; the lower part of the liquid storage connecting rod is movably connected with one end of the lever plate in the length direction; the lever plate is hinged to the bottom cylinder, and the other end of the lever plate movably penetrates through the bottom cylinder and is movably connected with the bottom of the driving rod on the outer side of the bottom cylinder. One end of the cylinder cover is hinged to the bottom cylinder, the hinged position is arranged at the end where the driving rod is located, and the other end of the cylinder cover is detachably connected with the bottom cylinder. An annular water pipe is arranged on the inner side of the cylinder cover, and multiple nozzles are arranged on the inner side of the annular water pipe at intervals. According to the device, by changing the placing structure of the filter paper, an operator can better replace the filter paper and stem cell tissues, and the stem cell tissues can be protected while the stem cell filtering and separating effect is improved.

Disclosed herein are compositions and methods for identifying materials suitable for functional delivery of a bioactive agent to a target tissue. These compositions and methods have the advantage of simultaneously screening a library of materials for the ability to deliver a bioactive agent to a cell, tissue, or organ. The compositions and methods can also be used to confirm that the agent is delivered in a manner sufficient for function of the agent.

The present invention relates to specific natural or artificial RNA or DNA / RNA substrates for cleaving by a Cas nuclease. The invention furthermore relates to a complex comprising the specific artificial RNA or DNA / RNA substrate, at least one of a Cas nuclease enzyme and at least one preselected guide RNA binding to at least one target RNA. The present invention also relates to methods for cleaving the natural or artificial RNA or DNA / RNA substrate and methods for detecting at least one target RNA in a cell, tissue, cellular nucleus, and / or sample using the substrate or for eliminating a cell expressing the at least one target RNA.

The invention discloses a WebXR-based immersive scientific experiment demonstration system and method, and the system comprises a tube body, the two ends of the tube body are of an opening structure, the top opening of the tube body is a first opening of a flat elliptical structure, the bottom end of the tube body is connected with a nut, the inner wall of the top opening of the tube body is provided with a first tooth block, and the outer wall of the top of the tube body is provided with a second tooth block. The second tooth blocks are located at the bottom of the first opening at intervals. The first tooth block is arranged at the first opening, so that when the needle head is drawn back and forth, the first tooth block can rapidly cut tissues, the tissues fall off and enter the first opening, and rapid extraction of the cell tissues is realized; the second tooth block can further cut the tissue, the cutting range is widened, and the tissue falling speed is increased; the first tooth block and the second tooth block can hook and store the tissue, so that the tissue can be collected in the tube body, and the cut tissue is prevented from being separated; and the fallen tissues can be quickly guided into the tube body and are prevented from being adhered to the outer wall of the tube body.

The utility model relates to a portable embryo transfer device, aims at solving the technical problem that the current embryo transfer device is used in the process, through Pasteur pipette to hollow capsule is pressed, embryo is extracted and is transferred, and the precision of the staff is not good at the squeezing degree of the empty capsule, and it is often possible to be too violent and the cell tissue and liquid are sucked into the pipe body together, thereby influence the final sampling result, including the pipe body, its characterized in that, both ends of the pipe body are open setting, the pipe body is transparent pipe, one end of the pipe body is connected with the pipe head, the pipe body is connected with the elastic empty capsule away from the pipe head one end, the inner chamber sliding connection of pipe body has the piston, the fixed disc is fixedly installed in the pipe body above the piston, and the fixed disc is connected with the spring between the piston, the utility model has the accurate control elastic empty capsule squeezing degree, and then avoids the cell tissue and liquid to be sucked into the pipe body together and influence the final sampling result.

1. The name of the product of this design: Oscillating incubator (horizontal). 2. Purpose of the product of this design: A culture device for culturing cells, tissues, and organisms by controlling parameters such as temperature, humidity, light, CO2 concentration, and pH. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The invention relates to modified apolipoprotein with a targeting body. The targeting body may for example be an antibody or antigen binding fragment that allows targeting of e.g. a specific cell, tissue or organ. The modified apolipoprotein can be used as a carrier for a payload as such or when incorporated in a lipid nanoparticle. The modified apolipoprotein finds use in the treatment or prevention of diseases, or targeting a pay load to a specific target site.

A biological system for generating and preserving a repository of personalized, humanized transplantable cells, tissues, and organs for transplantation, wherein the biological system is biologically active and metabolically active, the biological system having genetically reprogrammed cells, tissues, and organs in a non-human animal for transplantation into a human recipient, wherein the non-human animal does not present one or more surface glycan epitopes and specific sequences from the wild-type swine's SLA is replaced with a synthetic nucleotides based on a human captured reference sequence from a human recipient's HLA.

The invention relates to a baculoviral vector system for improved delivery of DNA, RNA and protein into mammalian cells, including primary cells, tissues, and whole mammalian organisms.

The disclosure relates to compositions and methods for enhancing asymmetric division of satellite cells and promoting muscle cell / tissue regeneration, for treatment of muscle tissue injuries and muscle diseases, including muscular dystrophies.

There are provided herein, inter alia, complexes, compositions and methods for the delivery of therapeutic, diagnostic and imaging agents, including nucleic acid, into a cell. The complexes, compositions and methods may facilitate complexation, protection, delivery and release of oligonucleotides and polyanionic cargos into target cells, tissues, and organs both in vitro and in vivo.

The invention discloses a tissue separator for child operating room nursing, which comprises a refining rolling structure, a test tube clamping assembly, a clamping base, a main body bracket, a supporting top plate and a rolling assembly supporting arm, and belongs to the technical field of tissue separation. On one hand, the pressure of the cell tissues between the fixing disc and the nylon net can be increased, and on the other hand, the contact area of the cell tissues and the nylon net can be increased, so that the cell tissues form a cell suspension more quickly, and the cell suspension enters the cell suspension storage box through the nylon net to be stored; and the cell suspension can be discharged through the valve on the liquid outlet at the bottom, so that the cell suspension enters the test tube below.

The invention belongs to the technical field of biomedical engineering and microfluidics, and particularly discloses a double-channel vascularization organ chip which comprises a channel layer and a bottom layer which are arranged in a stacked mode, and the lower surface of the channel layer and the bottom layer are sealed through bonding; a culture solution channel is arranged on the lower surface of the runner layer, snakelike pipelines are symmetrically arranged on the culture solution channel, and a first liquid injection channel and a second liquid injection channel are arranged at the two ends of the culture solution channel respectively; the lower surface of the runner layer is provided with a first cell culture chamber and a second cell culture chamber which are communicated with the culture solution channel; by adopting a double-layer structure of the flow channel layer and the bottom layer, the micro-fluidic chip has the characteristics of simple structure, convenience in operation, easiness in manufacturing of the chip and the like, and the first cell culture chamber and the second cell culture chamber are separated by the second micro-column; the interaction among different types of cell tissues in the first cell culture chamber and the second cell culture chamber can be realized by utilizing the gaps among the second micro-columns, so that the complex interaction among multiple tissues in the body can be really simulated.

Macromolecule compositions and related methods that effect targeted delivery of therapeutic agents to effector targets in a desired cell, tissue and / or organ of interest while minimizing or avoiding undesirable delivery to other cells, tissues or organs are provided. Compositions and methods related to macromolecules, such as an ANDbody™, that include an effector target binding domain specific for an effector target, and an address binding domain specific for an address target are described. The macromolecules are linked to small molecules.