198 results about "Cell organisation" patented technology

A system has been constructed that recapitulate the features of a capillary bed through normal human tissue. The system facilitates perfusion of three-dimensional (3D) cell monocultures and heterotypic cell co-cultures at the length scale of the capillary bed. A major feature is that the system can be utilized within a “multiwell plate” format amenable to high-throughput assays compatible with the type of robotics commonly used in pharmaceutical development. The system provides a means to conduct assays for toxicology and metabolism and as a model for human diseases such as hepatic diseases, including hepatitis, exposure-related pathologies, and cancer. Cancer applications include primary liver cancer as well as metastases. The system can also be used as a means of testing gene therapy approaches for treating disease and inborn genetic defects.

The invention provides articles of manufacture comprising biocompatible nanostructures comprising nanotubes and nanopores for, e.g., organ, tissue and/or cell growth, e.g., for bone, kidney or liver growth, and uses thereof, e.g., for in vitro testing, in vivo implants, including their use in making and using artificial organs, and related therapeutics. The invention provides lock-in nanostructures comprising a plurality of nanopores or nanotubes, wherein the nanopore or nanotube entrance has a smaller diameter or size than the rest (the interior) of the nanopore or nanotube. The invention also provides dual structured biomaterial comprising micro- or macro-pores and nanopores. The invention provides biomaterials having a surface comprising a plurality of enlarged diameter nanopores and/or nanotubes.

The present application relates to novel excitable particles which can be used in the health sector. It more particularly relates to particles which can generate electrons and/or high energy photon when excited by ionizing radiations such as X- Rays, γ-Rays, radioactive isotope and/or electron beams, and to the uses thereof in health, in particular in human health. The inventive particles are made of an inorganic material comprising oxygen, in particular an oxide, said material having an adequate density, and can be activated in vitro, ex vivo, or in vivo, by controllable external excitation, in order to disturb, alter or destroy target cells, tissues or organs. The invention also relates to methods for the production of said particles, and to pharmaceutical or medical device compositions containing same.

The present invention provides a novel microscale fluid handling system for initiating, culturing, manipulating and assaying three-dimensional multicellular assembly. The system including a microfluidic device and three-dimensional multicellular tissue surrogate assembly. The device of the invention includes at least one microfluidic channel; and at least one chamber, wherein the walls of the chamber are lined with a cell layer; and wherein fluid medium flows through each of the channels and chambers. Also, disclosed are methods for using the device to introducing test agents to the multicellular assemblies to observe biological responses thereof.

The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. Pre-designed barcodes are associated specific molecular targets through sequential hybridization experiments. A pseudo-color based barcoding scheme is developed to overcome limitations in the previous generation of the technology such as lack of visual signals that can be associated with the probes or small internal within cell when carrying out in situ experiments. The current method can be applied to both in vitro and in situ analysis. According to the method, each barcoding round comprises multiple serial hybridizations where a small number of colored signals (that are associated with probes) are used in each hybridization experiment within a serial hybridization round. Images from each serial hybridization experiment within the same serial hybridization round are combined to form a composite image for each barcoding round. In each barcoding round, the same set of molecular targets are analyzed. After all barcoding rounds are completed, associated of the barcode with these molecular targets is completed.

The invention discloses a micro-flow control chip for cell tissue culture and real-time monitoring and a use method thereof. The chip comprises a glass substrate layer and a PDMS micro-flow channel layer positioned on the glass substrate layer; the glass substrate layer comprises a glass substrate and a plurality of pairs of microelectrodes arranged on the glass substrate layer; the PDMS micro-flow channel layer comprises a plurality of independent micro-fluidic channels; the microelectrodes on the glass substrate are corresponding to the micro-flow channels on the PDMS micro-flow channel layer one by one; the microelectrodes are electrically connected with an external circuit. The use method comprises the steps of cell capture, cell tissue culture, electrical impedance spectroscopy detection and tissue release. The chip disclosed by the invention is processed by a transparent substrate, microscopic imaging can be taken as a supplementary means of the electrical impedance spectroscopydetection, and the dynamic growth and the physiological behavior of cell tissues and other biological processes are subjected to observation and in-situ analysis. The micro-flow control chip can be used in the fields of biological research, drug screening and the like of cells or tissues.

Disclosed are methods and compositions containing functionalized nanoporous silicon particles, useful in a variety of diagnostic and/or therapeutic regimens for delivery of genetic constructs to one or more cells, tissues, and/or organs of interest. Also provided are methods for introducing into selected host cells one or more selected nucleic acid molecules. The present disclosure is also directed to a method of treating a tumor, comprising the step of administering to an individual one or more of the compositions and formulations thereof as described herein.

The present invention is directed to various methods involving nonlinear microscopy and dyes that are sensitive to fast cellular membrane potential signals and capable of generating nonlinear optical signals. The present invention includes methods of producing high spatiotemporal resolution images of electrical activity in cellular tissue, as well as methods of detecting and investigating disease within a particular cellular tissue of a living organism. The present invention further relates to methods of detecting membrane potential signal changes in a neuron or a part of a neuron, as well as in a population of cells.

The invention relates to activatable novel particles which can be used in the health sector. The invention more particularly relates to composite particles which can generate free radicals or heat when excited by X-rays. The invention also relates to the uses thereof in human health. The inventive particles comprise an inorganic-based, and optionally organic-based, nucleus and can be activated in vivo in order to mark or affect cells, tissue or organs. The invention further relates to methods for the production of said particles, and pharmaceutical or diagnostic compositions containing them.

The present invention belongs to the technical field of cell biology and particularly relates to a technology for regulating and controlling a Jak-Stat pathway to differentiate, dedifferentiate and rejuvenate cells, and an application of the technology. Rejuvenated cell products and/or cell products of different lineage types are obtained by small molecule compound combination, cytokine combination or recombinant protein combination, a gene editing technology and a transgenic technology to quantitatively and/or regularly regulate and control gene or protein targets of the Jak-Stat signaling pathways in cells. The provided technology for regulating and controlling the Jak-Stat signal pathway, and the product/derivatives of the cell product can be used for re-programming cells, tissues, organs and organisms, construct tissue engineering materials, repair damages of tissues and organs of mammals and the aged and degenerated tissues and organs, delay or reverse aging processes of the cells, tissues, organs and organisms, and can be used for immunoregulation of the cells, tissues, organs and organisms.

The invention relates to a biphysic tissue engineering joint scaffold as well as a preparation method and an application thereof. The biphysic tissue engineering joint scaffold consists of a phrenology scaffold and a cartilage scaffold, wherein the cartilage scaffold is a PGA/PLA scaffold; the phrenology scaffold is a PCL/HA scaffold. The invention also provides a biphysic cell-tissue engineering joint scaffold constructor as well as a preparation method and an application thereof. The biphysic tissue engineering joint scaffold as well as the preparation method and the application thereof have the advantages that the biphysic tissue engineering joint scaffold is manufactured by utilizing a CAD/CAM technology, the external form and interior structure are accurate and controllable, the phrenology and the cartilage are matched with each other, the biphysic cell-tissue engineering joint scaffold constructor is formed by compositing seed cells by utilizing a tissue engineering technology, the tissue engineering joint can be regenerated in vivo and has the biological and mechanical biomechanical characteristics of the normal joint, an interface of a regenerated bones and the cartilage is well integrated, and the possibility is provided for performing biological reconstruction through the tissue engineering joint instead of the existing artificial joint.

A device for transporting at least one cellular entity during culture or maturation, the device comprising a substrate having one or more wells, said one or more wells being adapted to hold a cellular entity in a fluid, lid means for preventing entry or exit of the cellular entity from the one or more wells and fluid transport means connecting the one or more wells to enable flow of fluid or diffusion of chemical species. The apparatus alternatively or in addition comprise a module for transporting a payload at a controlled temperature, the module comprising an outer housing, an outer thermally insulating region, an inner thermally insulating region, and a heat sinking region located between the inner and outer thermally insulating regions, the inner thermally insulating region defining a cavity for receiving a payload, and heating means.

A microfabricated multi-tissue system for in vitro drug toxicity testing having a plurality of layers, each of which is formed of decellularized tissue extracellular matrix (ECM) including parenchymal cells and non-parenchymal cells attached thereto. Also disclosed is a method for producing a decellularized ECM paste, and methods for producing an ECM construct and a porous 3-D scaffold from the decellularized ECM paste.

The invention discloses an automatic extraction method for nucleic acid in an FFPE (Formalin-Fixed and Parrffin-Embedded) sample. When DNA or RNA of FFPE is independently extracted, cell tissue can beconfined in a certain space range by magnetic beads through a magnetic separation device, the consequence that tissue is sucked off along with a dewaxing agent or other solution can be effectively avoided, and furthermore the DNA or RNA of FFPE can be extracted; in co-extraction of the DNA or RNA of FFPE, the tissue is dewaxed and rehydrated along with the magnetic beads, lysate RTL is added, andthen RNA can be released into the solution. A centrifugal tube is placed in the magnetic separation device, digestion lysate with the RNA is transferred into a new centrifugal tube by using a liquidtransferring device, and then RNA extraction can be carried out; lysate DTL is continuously put into the magnetic beads into which the cell tissue is adsorbed in the original centrifugal tube, then the DNA can be released into the solution, and DNA extraction can be carried out.

A curtain-type anchoring biological culturing reactor and its method for culturing vegetable cell, tissue or organs are disclosed. The reactor consists of bed(E), curtain-type netted double-layer anchoring culturing bed(A), supply system(B), hanger(C), composite circulation feeder(F) and disposable sterile sealing film(G). (A) is hung on (C) and has (E), (F) is arranged on top of (E), (E) is sleeved with (G). It's simple, safe and cheap. It has less consumption and can be used for continuous production.

The invention discloses a 3D printing method for MC (methyl cellulose) based hydrogel bio-ink. MC hydrogel is the best matrix to obtain regenerative medicine application cell slices. However, a preparation method of the MC hydrogel currently only allows obtain geometric shapes with standardization and simplicity (namely, geometric shapes of container generated by the hydrogel). By use of the MC hydrogel, corresponding cell plate engineering and 3D printing of complicated shapes of biological tissues and organs can be realized through the 3D printing method. The stability and the rheological property of the method is suitable for an application of biological plate engineering under 37 DEG C. The cells are embedded in the MC based hydrogel, the cell viability is detected to be larger than 90%; the bio-imprinting survival rate of C2C12 cells embedded into the MC based hydrogel is higher than 80% by use of optimized printing parameters.