75 results about "Cell organisation" patented technology

The present invention relates to specific natural or artificial RNA or DNA / RNA substrates for cleaving by a Cas nuclease. The invention furthermore relates to a complex comprising the specific artificial RNA or DNA / RNA substrate, at least one of a Cas nuclease enzyme and at least one preselected guide RNA binding to at least one target RNA. The present invention also relates to methods for cleaving the natural or artificial RNA or DNA / RNA substrate and methods for detecting at least one target RNA in a cell, tissue, cellular nucleus, and / or sample using the substrate or for eliminating a cell expressing the at least one target RNA.

The utility model relates to a portable embryo transfer device, aims at solving the technical problem that the current embryo transfer device is used in the process, through Pasteur pipette to hollow capsule is pressed, embryo is extracted and is transferred, and the precision of the staff is not good at the squeezing degree of the empty capsule, and it is often possible to be too violent and the cell tissue and liquid are sucked into the pipe body together, thereby influence the final sampling result, including the pipe body, its characterized in that, both ends of the pipe body are open setting, the pipe body is transparent pipe, one end of the pipe body is connected with the pipe head, the pipe body is connected with the elastic empty capsule away from the pipe head one end, the inner chamber sliding connection of pipe body has the piston, the fixed disc is fixedly installed in the pipe body above the piston, and the fixed disc is connected with the spring between the piston, the utility model has the accurate control elastic empty capsule squeezing degree, and then avoids the cell tissue and liquid to be sucked into the pipe body together and influence the final sampling result.

The invention relates to modified apolipoprotein with a targeting body. The targeting body may for example be an antibody or antigen binding fragment that allows targeting of e.g. a specific cell, tissue or organ. The modified apolipoprotein can be used as a carrier for a payload as such or when incorporated in a lipid nanoparticle. The modified apolipoprotein finds use in the treatment or prevention of diseases, or targeting a pay load to a specific target site.

There are provided herein, inter alia, complexes, compositions and methods for the delivery of therapeutic, diagnostic and imaging agents, including nucleic acid, into a cell. The complexes, compositions and methods may facilitate complexation, protection, delivery and release of oligonucleotides and polyanionic cargos into target cells, tissues, and organs both in vitro and in vivo.

The production method includes: linearly printing a plurality of cells between a first substrate and a second substrate in such a manner that both ends of the plurality of cells are supported on the first substrate and the second substrate, respectively; obtaining a plurality of cell tissues T by culturing the printed plurality of cells; filling a soluble liquid holding material between the first substrate and the second substrate; curing the filled holding material; separating each of the plurality of cell tissues T from at least one base material; and obtaining a plurality of blocks 50C by dividing the holding material along an extending direction of the plurality of cell tissues T held by the cured holding material. The obtaining of the plurality of blocks 50C includes housing the cured holding material in a dividing housing 30 provided with a plurality of blades 31.

The utility model relates to the technical field of cell culture, and discloses a cell shake flask capable of improving tissue digestion efficiency, which comprises a culture flask body, a flask cover is mounted at the top end of the culture flask body, and a first constant-temperature heating cover is arranged on one side of the outer side wall of the culture flask body. Mounting blocks are fixed to the front end and the rear end of one side of the first constant-temperature heating cover correspondingly, inner cavities are formed in the first constant-temperature heating cover and the second constant-temperature heating cover correspondingly, and electric heating wires are mounted in the inner cavities. When the culture bottle body disclosed by the utility model is used for culturing cells, the culture bottle body can be heated according to actual conditions by arranging components such as the first constant-temperature heating cover, the mounting block, the mounting groove and the second constant-temperature heating cover, and meanwhile, the digestion efficiency of cell tissues can be promoted by heating the culture bottle body; and the culture bottle body can be detached when not needed subsequently, so that the observation of the condition of cells in the culture bottle body through the culture bottle body is not influenced, and the use effect is better.

Provided are methods for rapid multiplex HLA genotyping and transcript quantitation. In some embodiments, the presently disclosed methods include providing a. sample from the cell, tissue, or organ that has mRNA derived from an HLA gene product; reverse transcribing the mRNA to produce a. pool of cDNAs; amplifying the cDNAs that result from reverse transcription of HLA-specific mRNAs to produce a pool of HLA-specific cDNAs; and determining the sequence of each HLA-specific cDNAs, whereby the HLA status of the cell, tissue, organ, or subject is determined. Also provided are methods for genotyping donor cells, tissues, and / or organs meant for transplantation into recipients. In some embodiments, the methods can be used to identify the presence of absence of each of HLA-A, -B, -C, -DRB 1 / 3 / 4 / 5, -DQA1, and -DQB1 in a biological sample.

The present disclosure provides methods for inducing cellular quiescence—defined as a reversible arrest of cell proliferation—by inhibiting ribosome biogenesis. The methods involve administering a quiescence-inducing agent, such as a small molecule inhibitor, peptide, antisense oligonucleotide, or targeted degrader, that interferes with rRNA transcription, processing, ribosomal protein synthesis, or ribosome assembly. The agent may be applied to cultured cells, tissues, organs, or organoids, across a range of cell types and species. In some embodiments, the methods reduce metabolic activity and enhance stress resistance in non-dividing or post-mitotic cells. By slowing proliferation and / or metabolism while preserving viability, these methods enable improved preservation of cells, tissues, and organs-enhancing transplantation logistics, biobanking, regenerative medicine workflows, and biological transport. Additional applications include studies of cell cycle regulation, tissue regeneration, stress biology, aging, and induction of organismal hypometabolism (e.g., torpor or hibernation). The disclosure includes examples demonstrating quiescence induction using chemical biology systems, pharmacological agents, and genetic approaches.

The present disclosure is in the field of culturing a homocellular cell culture of progenitor cells form a heterocellular tissue sample. The present disclosure relates to a method for specifically generating muscle progenitor cells or adipogenic progenitor cells from an isolated tissue sample, preferably a muscle tissue sample, e.g., from mammalian origin, preferably from bovine, ovine, murine, or porcine origin. The method includes culturing a heterocellular tissue sample comprising a muscle progenitor cell and an adipogenic progenitor cell in a selection medium including comprsing at least one compound which specifically promotes the proliferation of muscle progenitor cells or of adipogenic progenitor cells leading to a homocellular cell culture. Further, the present disclosure relates to the selection medium which facilitates selective generation of muscle progenitor cells or of adipogenic progenitor cells. The method and selection medium of the invention can be used for producing homocellular cell cultures of muscle progenitor cells or of adipogenic progenitor cells. The homocellular cell cultures produced according to the present disclosure can be used to produce a cell culture-based meat product, preferably for animal or human consumption.

This disclosure provides compositions, systems, and methods for targeting, editing, modifying, or manipulating the genome of a host cell at one or more locations in a DNA sequence within a cell, tissue, or object. A genetically modified system for treating phenylketonuria (PKU) is described. In one embodiment, this disclosure relates to a pharmaceutical composition comprising the above system and a pharmaceutically acceptable excipient or carrier, wherein the pharmaceutically acceptable excipient or carrier is selected from the group consisting of plasmid vectors, viral vectors, vesicles, and lipid nanoparticles.

The invention relates to a cell tissue culture device special for primary cells, and relates to the technical field of cell culture, the cell tissue culture device comprises an incubator, the interior of the incubator is divided into three independent culture chambers through partition plates, each culture chamber is internally provided with at least three culture dishes, and the cell tissue culture device further comprises a dynamic culture assembly, at least two culture rooms are provided with a dynamic culture assembly, the dynamic culture assembly is provided with a micro-channel structure capable of simulating blood vessels, the dynamic culture assembly, the electrophysiological stimulation piece and the traction force simulation piece are integrated to form the culture system capable of simulating various physiological conditions in the body, the dynamic culture assembly simulates a blood vessel microchannel to realize low-shear-force continuous perfusion; the electrophysiological stimulation piece simulates a neuroelectric signal; the traction force simulation part applies controllable mechanical traction force through a multi-caliber breather pipe, and the three parts have a synergistic effect, so that the physiological function expression and culture quality of the primary cells are improved.

A novel ubiquitous chromatin opening element (UCOE) named SRF-UCOE and methods for its use are provided. Compositions including recombinant and synthetic SRF-UCOE nucleic acid molecules, DNA constructs and vectors comprising the SRF-UCOE nucleic acid molecules, host cells comprising the DNA constructs or vectors, and cell culture systems comprising such host cells. SRF-UCOE polynucleotide sequences can be used in DNA constructs or expression cassettes for transformation and expression in cells or organisms of interest. The compositions and methods provided are useful for increasing and / or maintaining expression of a gene of interest. Transgenic cells, tissues, and animals comprising a SRF-UCOE nucleotide sequence are also provided. Methods are provided for increasing and / or maintaining expression of a gene of interest and for treating a subject via gene therapy.

The present invention relates to the technical field of microbial single-cell sequencing and in particular to a single-cell tissue in-situ sequencing method based on Raman spectroscopy sorting technology. The method comprises: preserving the position information of microbial single cells in a tissue by means of tissue sectioning, and identifying and sorting the microbial single cells in the tissue in a section on the basis of Raman spectroscopy to obtain desired target single cells, without relying on labeling with probes and the like, thereby realizing in-situ detection of the single cells at the tissue level and obtaining a single-cell genome and single cell transcriptome information. The microbial morphology of the desired tissue section is directly observed under a microscope and Raman sorting is performed, without the need for steps such as enzyme penetration, digestion, and labeling, and thus, the operation is simple. The microbial single cells in the tissue are precisely sorted by means of the cell morphology and spectral comparison, and the species are identified by means of transcriptome analysis, so that the transcriptional change of genes at the microbial single-cell level during host-microbe interaction can be revealed.

To provide methods and compositions for modulating a target genome.SOLUTION: The present disclosure relates to compositions, systems, and methods for targeting, editing, modifying, or engineering a DNA sequence (e.g., inserting a heterologous DNA sequence of interest into a target site of a mammalian genome) at one or more locations within a DNA sequence in a cell, tissue, or subject, e.g., in vivo or in vitro. The DNA sequence of interest may comprise, for example, a coding sequence, a regulatory sequence, a gene expression unit.SELECTED DRAWING: None

Provided is a cell organization reactor with which it becomes possible to produce a cell population having an organized structure such as a biological tissue. A cell organization reactor 10 comprises a culture vessel 20, a culture medium container 30, and a control unit 40. The culture vessel 20 accommodates a cell sheet laminate 50 in which a first cell sheet 52 and a second cell sheet 54 are laminated on each other. A culture medium stored in the culture medium container 30 is supplied to the cell sheet laminate 50 in the culture vessel 20 via a culture medium intake pipe 60.

To provide compounds, compositions, and methods for improving the viability and / or function of cells or for the in vitro, ex vivo or in vivo protection of cells, tissues, grafts, or organs from various damages.SOLUTION: The reagents and compositions are based on activation of the heat shock response and / or the antioxidant response, contain, for example, HSP90 co-factor inhibitor such as Celastrol or Celastrol analogs, and are used alone or in combination with an adjunct agent (e.g. an NRF-2 activator, antioxidant, and the like). Therapeutic enhancement may also include increase in paracrine effector production and signaling. Also described are methods for improving the resistance of cells, tissues, grafts, or organs to damages or stress, such as hypoxic or oxidative stress-induced cell death, and / or for improving the viability and retention of transplanted or transfused cells. Also provided are the therapeutic treatment or prevention of ischemic injury (e.g. myocardial infarct, ischemia / reperfusion injury) and related stressors (hypoxia, oxidative stress, inflammation, sepsis / shock, and the like).SELECTED DRAWING: None

The invention discloses an intercellular communication network decoding method, device, equipment and medium, and relates to the technical field of intercellular communication.The intercellular communication network decoding method comprises the steps that space transcriptome data of a single cell tissue sample is obtained, and candidate gene pairs are screened out through gene expression correlation; dividing intercellular communication of the candidate gene pair into a plurality of communication modes by combining gene expression distribution space distance characteristics and replacement test; performing nonlinear transformation and dimensionality reduction on the initial expression features of each gene, aggregating feature information of corresponding neighbor nodes according to a communication mode by using a multi-layer multi-relational graph convolutional network, and outputting multi-scale features; performing adaptive weighted fusion on the multi-scale features by using a multi-head attention mechanism; and predicting and integrating the existence probability and the communication mode of the intercellular communication relationship to obtain a decoding result of the intercellular communication network. In this way, through fusion expression and spatial features, multi-module modeling is coordinated, and accurate recognition of multi-mode intercellular signal transmission events under different spatial scales is achieved.

The utility model relates to an adhesive biological sample collecting tube which comprises a tube body and a tube cover, one end of the tube body is closed, the other end of the tube body is open, the tube cover covers the open end of the tube body, an adsorption layer used for adhering a biological sample is arranged on the inner side of the tube cover, and a ruler used for marking the position of the biological sample is arranged on the tube cover. The arrangement position of the ruler is matched with the arrangement position of the adsorption layer, and the adsorption layer is located in the tube body when the tube cover covers the tube body. According to the utility model, an obtained sample can be accurately positioned under a microscope, a target tissue can be visually and secondarily collected, a target experiment scheme can be directly implemented in a collection tube, unused samples do not need to be taken out in advance, the cell collection efficiency is greatly improved, and compared with other existing cell tissue collection methods, the cell collection efficiency is greatly improved. And the loss rate of the cell sample is reduced by 50%.

The disclosure provides, e.g., compositions, systems, and methods for targeting, editing, modifying, or manipulating a host cell's genome at one or more locations in a DNA sequence in a cell, tissue, or subject. Heterologous gene modifying systems for treating cystic fibrosis are described.

The utility model is applicable to the technical field of single cell sequencing, and provides a separation device for single cell sequencing, which comprises a separation frame arranged on a rack and used for separating single cells, and a separation mechanism used for driving the separation frame to rotate to separate the single cells is arranged on the rack and the separation frame; the mixing vessel is arranged on the rack and is used for mixing tissues containing single cells and a solvent; the separation hopper is assembled on the rack, and a filter screen for separating a solution containing single-cell tissues is arranged in the separation hopper; and the extruding mechanism is arranged on the rack and is used for extruding and mixing the tissue containing the single cells and the solution in the mixing vessel. According to the separation device for single cell sequencing, provided by the scheme, single cell separation operation can be carried out, a worker can be helped to carry out pretreatment operation on a single cell solution, the operation burden of the worker is effectively reduced, and convenience is provided for the worker.

The utility model relates to the technical field of cell culture, and discloses a large-scale cell culture device which comprises a cell culture box body, a rotating mechanism is arranged in the cell culture box body, and a jacking mechanism is arranged in the cell culture box body. A culture dish can be conveniently placed in a placement groove of a rotating disc, the situation that after the hands of an operator go deep into the cell culture box body, the sight is influenced and other culture dishes are knocked over is prevented, the operator can conveniently place the culture dish, the rotating disc rotates slowly, and the situation that the culture effect is influenced due to too large centrifugal force of cells in the culture dish is prevented; when a nutrient solution needs to be placed, the culture dish is jacked from the bottom of the placing groove by utilizing the jacking block, so that the sliding plate is opened, and the nutrient solution is dropped into the culture dish without a large opening, so that bacteria in the air are prevented from falling into the culture dish to pollute cell tissues, and the cell culture effect is improved.

Disclosed are ATP-independent bioluminescent reporter systems for enhanced in vivo imaging. Also disclosed is a modified luciferin, potassiorin, comprising a potassium-binding moiety. Also disclosed is an engineered luciferase, BRIPO, optimized for synergistic interaction with potassiorin. A further version of the disclosed system produces bioluminescence signals responsive to physiological potassium concentrations. In further instances, the system enables real-time monitoring of K+ dynamics in live cells, tissues, and animals. A further embodiment includes applications in imaging neuronal activity, studying ion flux, and developing bioluminescent indicators for diverse analytes. The disclosed system addresses limitations of traditional imaging methods, offering improved sensitivity and biocompatibility.

The present disclosure relates generally to microspheres comprising decellularized tissue extracellular matrix, at least a natural biopolymer, and at least a crosslinking agent. In some embodiments, the microsphere comprises biological cells and / or at least a biomolecule. Moreover, the present disclosure also relates to the method of generating said microspheres and the uses thereof.

Provided are a fluorescent probe, a preparation method therefor and a use thereof. The fluorescent probe sensitively and specifically responds to viscosity, and can be used for the specific fluorescence labeling of proteins as well as in the quantification, detection or kinetic study of proteins and the imaging of cells, tissues and living bodies.

The invention belongs to the technical field of analytical chemistry, and particularly discloses a fluorescent probe and application of a preparation method thereof in detection of hydrogen peroxide, the fluorescent probe is high in sensitivity to hydrogen peroxide, interference of other active oxygen or biomolecules is avoided, and accurate detection of target molecules is achieved; the hydrogen peroxide probe has high hydrogen peroxide sensitivity while playing a role in cells, has good biocompatibility, and can be directly applied to real-time imaging and dynamic monitoring of hydrogen peroxide in living cells, tissues and even living bodies. The detection limit of the hydrogen peroxide is 142.18 nM, the hydrogen peroxide with the concentration of 0-1 mM can be detected, and the hydrogen peroxide fluorescent probe is in direct proportion to the fluorescence intensity of the hydrogen peroxide within the concentration range of 0-500 [mu] M. The method is simple, convenient and rapid to operate, instant fluorescence signal change can be generated after the reaction with hydrogen peroxide, real-time analysis of target molecules is realized, the detection efficiency is greatly improved, an effective research tool can be provided for researching the content of hydrogen peroxide in cells, and early warning can be provided for the field of clinical diagnosis.

The utility model application relates to the field of biological experiment equipment discloses a kind of living cell tissue perfusion collection systems.The system includes: liquid flow switching dosing module, constant temperature incubation module, sample automatic collection module and sample low-temperature maintenance module.Liquid flow switching dosing module switches different liquid according to experimental requirement anytime, average shunt pumps into constant temperature incubation module;Constant temperature incubation module provides closed, constant temperature incubation liquid flow environment for living cell tissue perfusion experiment;Sample automatic collection module receives the perfusion liquid sample flowing through constant temperature incubation module, and according to the specified time sequence, sample is collected into collection well plate;Sample low-temperature maintenance module keeps sample collection plate in relatively low temperature state by refrigeration temperature control mode, to delay sample volatilization and the denaturation of the substance to be tested in it, guarantee sample stability.Through the system, perfusion liquid sample can be accurately collected according to time sequence, improve experimental result accuracy, to obtain repeatable high-quality experimental result.

The utility model relates to the technical field of aerosol generation, and provides an aerosol generation product, the aerosol generation product comprises a granular or filamentous fuming unit, the fuming unit comprises a plant material with a cavitated cell tissue and a fuming medium, the cavitated cell tissue is filled with a part of the fuming medium, and the fuming medium is filled with a part of the plant material. Part of the fuming medium coats the outer surface of the plant material; and the fuming medium coated on the outer surface of the plant material is a gel film layer. According to the aerosol generating product provided by the invention, the gel film layer plays a role in isolation and protection, so that on one hand, a fuming medium in the cavitated cell tissue of the plant material is prevented from leaking out, and the aerosol generating product has better quality stability in the transportation and storage process; on the other hand, the hygroscopicity and the moisture absorption rate of the fuming unit are remarkably reduced, the fuming effect of the fuming unit is guaranteed, and the validity period of the fuming unit is effectively prolonged.