43 results about "Cell tissue culture" patented technology

The present invention relates to a three dimensional construct formed from non-biodegradable and non-cytotoxic polymers that provide an internal and external space for living cells to attach, proliferate and differentiate. The construct is composed of polymer struts and / or fibers which are joined together in a designed 3 dimensional pattern. The 3 dimensional cell culture construct (cell culture insert) is intended to be used together with cell / tissue culture plate, tissue culture flask, bioreactor and the like under normal cell culture conditions. The invention further provides methods of making the 3 dimensional cell culture construct. Finally, the invention provides kits comprising one or more 3 dimensional porous cell culture construct in a package together with other cell culture supplies, such as tissue culture plate and flasks.

A process for producing a collagen sponge and process for producing an artificial skin, in which a final collagen sponge and artificial skin can be obtained with high yield without occurrence of defectives through simplified steps; and a cell tissue culture substrate and artificial skin utilizing the collagen sponge produced by the above collagen sponge production process. There is provided a process for producing a collagen sponge, comprising using a dilute collagen solution having its pH adjusted with acetic acid.

The invention discloses a bio-macromolecular hydrogel prepared by enzyme catalysis and ion cross-linking, and a preparation method of the bio-macromolecular hydrogel. The bio-macromolecular hydrogel comprises a protein or polypeptide bio-macromolecular network formed by enzyme-catalyzed cross-linking of protein or polypeptide or amino acid residue-containing molecule, accounting for 1-99 wt% of total dry mass of the hydrogel; and a polysaccharide bio-macromolecular network formed by bivalent ion cross-linking of polysaccharide macromolecule, accounting for 1-99 wt% of total dry mass of the hydrogel. The above two networks are inter-penetrated without chemical bonding. The hydrogel has the advantages of excellent mechanical properties, no use of chemical cross-linking agent, simple and effective preparation method, good bio-compatibility and mechanical strength, and capability of steam sterilization; may be in the forms of wet or dry film, porous sponge, tube and particles; and can be used in cell / tissue culture, and used as tissue repair material, tissue engineered scaffold or drug release carrier.

The invention discloses a PEG-grafted polysulfone or polyethersulfone hollow fiber membrane and its preparation method and application. : After mixing in a ratio of 3 to 9, it is dissolved in a solvent such as N-methylpyrrolidone with a weight ratio of 15 to 30%, dissolved under stirring for 10 to 12 hours, and degassed to obtain a spinning stock solution. Two concentric hollow fiber spinnerets are used to extrude the spinning dope through the spinnerets at a speed of 5-20ml / min. The as-spun fibers travel 10-30cm in the environment, and then solidify and form in a water bath at a temperature of 50-80°C, with a fluid linear velocity of 5-40m / min. The hollow fiber membrane has the beneficial effect of anti-platelet, protein and small molecule adsorption, and can be used as a hemodialysis device, or used in bioartificial organs for tissue culture of various human or animal cells, drug metabolism, toxicity and pathology research.

The invention discloses a microfluidic chip for cell tissue culture and real-time monitoring and a method for using the chip. The chip includes a glass substrate layer and a PDMS microfluidic channel layer on the glass substrate layer. The glass substrate layer includes a glass substrate and a glass substrate. Several pairs of microelectrodes arranged on the glass substrate layer, the PDMS microfluidic channel layer includes several independent microfluidic channels, and the microelectrode pairs on the glass substrate correspond to the microfluidic channels on the PDMS microfluidic channel layer; The microelectrodes are electrically connected to external circuits. The methods of use include cell capture, cell tissue culture, electrical impedance spectroscopy detection, and tissue release. The chip of the present invention is processed with a transparent base material, and the microscopic imaging can be used as a supplementary means for electrical impedance spectroscopy detection to observe and analyze biological processes such as dynamic growth and physiological behavior of cell tissues in situ. It can be used in fields such as biological research of cells or tissues and drug screening.

A method for cultivating and breeding cell tissue of the tissue culture seedling of Dendrobium candidum comprises the steps of mother plant treatment, soaking disinfection, cleaning and cutting, callus induction, callus crushing, shake cultivation, protocorm cultivation, adventitious bud cultivation, plantlet cultivation and sapling cultivation. According to the invention, good characters of the previous generation are stably inherited by the next generation through a method of asexual reproduction, so that the phenomenon of character segregation of the filial generation, brought by sexual reproduction, can be avoided effectively, the characters of the mother plant can be reserved best, and the production of tissue culture seedling has purposiveness and pertinence.

The invention discloses a cell tissue culture system capable of accurately controlling air pressure. The cell tissue culture system comprises a culture room, a high pressure air source, an air flow meter, an air inlet pipeline, an air inlet switch, an air outlet pipeline, a pressure sensor, a decompression device, an exhaust valve and a control device, wherein the high pressure air source is communicated with an air inlet of the culture room through the decompression device, the air flow meter and the air inlet pipeline; the air inlet switch is arranged on the air inlet pipeline; an air outlet of the culture room is connected with the air outlet pipeline; the air outlet pipeline is communicated with the outside through the exhaust valve; the exhaust valve is used for controlling air in the culture room to be quantitatively updated; the air flow meter is used for measuring air intake flow in real time; the pressure sensor is used for sensing the air pressure in the culture room and on the air inlet pipeline and the air outlet pipeline; and the control device is used for controlling the air inlet switch to be turned on or off according to a signal from the pressure sensor. According to the cell tissue culture system, the air pressure in the culture room is stabilized to be in the preset level for a long time, and the air in the culture room is constantly updated to guarantee the accuracy of scientific research.

The embodiment of the invention discloses a method and device for preparing tissue. The method includes the steps that a structural shape is built according to a shape structure cultivated by cell tissue; a three-dimensional porous structure of the structural shape is built according to the structure characteristics of cells, wherein the structure characteristics comprise the structure layer numbers of the cells and the shapes of the cells; the three-dimensional porous structure is biologically printed into a cell culture body of a microenvironment system. The stable environment can be provided for growth of the cells accordingly, the tissue is close to the structure of tissue organs of the human body, the gap forming orientation is regular and ordered, and the problem that distribution of array culture cells is unordered can be solved; meanwhile, the method and device can be used for researching of some cell-culture-related tasks and medicine screening, and researchers are assisted in obtaining cell growth data closer to the normal physiological state.

The invention discloses a method for cultivating yew cell tissue culture. Specifically, yew seeds are sterilized, cleaned, soaked, and seed-coated, inoculated on an induction medium, and cultured in the dark. After the seeds germinate , cultivated into yew plants; select well-grown yew plants as explants; inoculate the explants on the medium, and cultivate them in the dark; then transfer them to a culture room with a temperature of 25°C and 28°C for cultivation; select vigorous growth , no browning, and yellow-white callus are inoculated into the subculture medium for periodic subculture; after that, select seeds, inoculate the seeds into the subculture medium, light culture, and collect after 30 to 35 days of culture , Taxus cell tissue culture. The paclitaxel content of the yew cell tissue culture provided by the present invention can reach 15‰-17‰, and the yew cell tissue does not brown during the passaging process, and the content of paclitaxel produced does not decrease, and the temperature and light conditions can still be changed. Steady growth.