9 results about "Cell tissue culture" patented technology

The utility model relates to the technical field of cell tissue culture, and discloses automatic liquid adding equipment for cell tissue culture, which comprises a cell culture chamber, an operation panel, a culture chamber main chamber, a chamber door, a moving component, a liquid suction device, a solution bottle, a support plate and a culture dish, the cell culture chamber is hermetically fixed at the right part of the upper end of the supporting plate, the operation panel is obliquely arranged at the upper part of the culture chamber main chamber, the chamber door is arranged on the front side of the cell culture chamber in an openable manner, and the chamber door and the cell culture chamber are kept sealed; the moving assembly is fixed to the right side of the upper portion of the culture room main chamber, the liquid suction device is fixed to the driving end of the moving assembly, multiple rows of culture dishes and multiple solution bottles are arranged at the upper end of the supporting plate, and reagents needing to be added are stored in the solution bottles. The cell tissue culture device can guarantee the stability of a culture environment, improve the culture quality of cell tissues, reduce the working intensity of testers and facilitate flexible selection of culture schemes.

The invention relates to the technical field of biology, in particular to a biomechanical system for cell tissue culture and biological material preparation, which comprises a reactor module, a power pressurization module, a monitoring sensor, a computer and a circulation module, the reactor module is arranged on the power pressurization module, the monitoring sensor is connected with the power pressurization module, and the computer is connected with the circulation module. The computer is connected with the monitoring sensor; the circulating module is connected with the sensor and the reactor module; the power pressurization module comprises a rack, a driving assembly, a screw and a pressure sensor, the screw and the driving assembly are installed on the rack, the driving assembly drives the pressure sensor to move through the screw, and in this way, the problems that in the prior art, a biomechanical device is too large in size, too heavy in mass, high in use cost and poor in practicability due to equipment integration are solved. The mechanical control precision is poor, and the pressurizing effect is single.

The invention relates to a cell tissue culture device special for primary cells, and relates to the technical field of cell culture, the cell tissue culture device comprises an incubator, the interior of the incubator is divided into three independent culture chambers through partition plates, each culture chamber is internally provided with at least three culture dishes, and the cell tissue culture device further comprises a dynamic culture assembly, at least two culture rooms are provided with a dynamic culture assembly, the dynamic culture assembly is provided with a micro-channel structure capable of simulating blood vessels, the dynamic culture assembly, the electrophysiological stimulation piece and the traction force simulation piece are integrated to form the culture system capable of simulating various physiological conditions in the body, the dynamic culture assembly simulates a blood vessel microchannel to realize low-shear-force continuous perfusion; the electrophysiological stimulation piece simulates a neuroelectric signal; the traction force simulation part applies controllable mechanical traction force through a multi-caliber breather pipe, and the three parts have a synergistic effect, so that the physiological function expression and culture quality of the primary cells are improved.

The invention discloses an extracorporeal circulation organoid 3D culture device based on a tumor microenvironment. The extracorporeal circulation organoid 3D culture device comprises a circulation driving module, a sample adding module and a 3D cell tissue culture module. The circulation driving module is a key for constructing extracorporeal circulation and drives a culture solution to flow in the device so as to achieve the purpose of simulating continuous flow of blood in a human body; the sample adding module is used for helping a culture solution in the device to metabolize and ensuring that components of the culture solution are normal all the time so as to achieve the effect of simulating blood components in a human body; the 3D cell tissue culture module is used for providing a culture environment with a 3D framework for the growth of tumor cells so as to ensure that the cells are cultured in the culture environment with the 3D framework. The circulation driving module, the sample adding module and the 3D cell tissue culture module are communicated through pipelines, so that a complete in-vivo blood circulation system is formed, better 3D in-vitro cell culture can be carried out, and the influence of blood circulation on tumor cell culture is effectively simulated.

The box is used for culturing and transporting cell tissue, and the closure is light-transmitting. 1.1) Top perspective view; 1.2) Top view; 1.3) Bottom view; 1.4) Front view; 1.5) Back view; 1.6) Right side view; 1.7) Left side view; 1.8) Bottom perspective view.

The present invention is specifically a biological cell stimulation system based on a pneumatic annular mold and a control method thereof, comprising: an annular culture mold, an air pump device; the annular culture mold is arranged in a culture dish and connected to the air pump device through an air duct; the annular culture mold comprises: an upper mold, a lower mold and a mold base; the upper mold and the lower mold, and the lower mold and the mold base are sealed and connected to form an annular culture mold with a variable volume; the mold base is arranged in a culture dish; a through hole is provided on the upper mold, and the air pump device is connected to the lower mold through the air duct through the through hole. Since the present invention has no special requirements in the application of promoting differentiation of cell tissue culture, there is no need for complex processing of cell samples, and the integration of culture and stimulation can be realized, which effectively reduces the damage and infection probability of cell tissues during the culture and stimulation process, and is used in skeletal muscle tissue engineering, drug screening, and the research of life-like robots with muscle cells as driving units.

A cell tissue culture and transport dish comprising: a dish body having an accessible interior and a top opening; a filter disposed within the dish body and communicating the interior of the dish body with an exterior of the dish body, the filter being configured to permit gas exchange between the interior and exterior of the dish body while preventing contamination of the cell tissue culture; a closure means for closing the top opening of the dish body, the closure means being fluid- and gas-tight; and at least one aseptic fluid connection through the dish body, said at least one aseptic fluid connection including two fluid inlet connections and one fluid outlet connection. Abstract figure: Figure 1

The invention belongs to the technical field of cell tissue culture, and discloses a tissue engineering blood vessel culture method and device, electronic equipment and a storage medium, and the method comprises the following steps: obtaining a target pressure waveform suitable for cell growth in tubular tissue, decomposing the target pressure waveform into a plurality of sine components through a Fourier transform method, the waveforms of the plurality of pressure waveform adjusting devices are respectively adjusted to be a plurality of corresponding sine components so as to be superposed to form the actual pressure waveform of the tubular tissue, the actual pressure waveform is used for stimulating cell growth in the tubular tissue to be reconstructed into the tissue engineering blood vessel, and the actual pressure waveform of the tubular tissue is monitored according to the target pressure waveform; by means of the Fourier transform method, the waveforms of the multiple pressure waveform adjusting devices are adjusted into the corresponding sine components, so that the pressure waveforms suitable for cell growth in the tubular tissue are obtained through adjustment, the culture efficiency of the tubular tissue is improved, the culture time is shortened, and the mechanical property of the tissue engineering blood vessel is improved.

The invention discloses a cell tissue culture device and a manufacturing method thereof. The cell tissue culture device comprises a culture bottle; bottle caps are arranged at the tops of the culture bottles; an air pipe is arranged at the top of the bottle cap; the air pipe downwards extends into the culture bottle; a plurality of layers of absorbent cotton and a plurality of layers of activated carbon are arranged in the air pipe; the absorbent cotton and the activated carbon are sequentially staggered from bottom to top; a limiting bracket is arranged in the air pipe; the limiting support is located below the absorbent cotton on the lowermost layer. By arranging the air pipe, the degreasing cotton, the activated carbon, the limiting bracket, the needle type filter and the like, filtered air, carbon dioxide and other gases required by cell tissue culture can be introduced into the culture bottle, so that a microenvironment closer to in-vivo survival conditions can be provided for cells, and the cells can grow quickly.