43 results about "Cell tissue culture" patented technology

The invention discloses a bio-macromolecular hydrogel prepared by enzyme catalysis and ion cross-linking, and a preparation method of the bio-macromolecular hydrogel. The bio-macromolecular hydrogel comprises a protein or polypeptide bio-macromolecular network formed by enzyme-catalyzed cross-linking of protein or polypeptide or amino acid residue-containing molecule, accounting for 1-99 wt% of total dry mass of the hydrogel; and a polysaccharide bio-macromolecular network formed by bivalent ion cross-linking of polysaccharide macromolecule, accounting for 1-99 wt% of total dry mass of the hydrogel. The above two networks are inter-penetrated without chemical bonding. The hydrogel has the advantages of excellent mechanical properties, no use of chemical cross-linking agent, simple and effective preparation method, good bio-compatibility and mechanical strength, and capability of steam sterilization; may be in the forms of wet or dry film, porous sponge, tube and particles; and can be used in cell/tissue culture, and used as tissue repair material, tissue engineered scaffold or drug release carrier.

The invention relates to a device and method for noninvasive continuous monitoring of quantity or concentration of dynamic cells, in particular to a device and method for noninvasive continuous on-line or off-line monitoring of quantity or concentration of dynamic cells in adherent cell cultures (usually as stem cell cultures or other therapeutic cell cultures) or cell and tissue cultures. The device comprises: a cell culture medium supply system, one/one type of or multiple/multiple types of upstream biomedical indicator detection head(s) or connector(s), a cell culture , one/one type of or multiple/multiple types of downstream biomedical indicator detection head(s) or connector(s), one or multiple speed controllable sterile driving unit(s) of fluid, a waste or harvested liquor system, liquid conveying pipeline systems that are driven and controlled in speed by the speed controllable sterile driving unit(s) of fluid for connecting the above components in order. And the upstream and downstream biomedical indicator detection head(s) or connector(s) are connected to a monitoring and controlling system and/or a computer so as to obtain, process and monitor data, and furthermore provide feedbacks for the whole cell culture system through a signal feedback system for realizing control.

The invention discloses a micro-flow control chip for cell tissue culture and real-time monitoring and a use method thereof. The chip comprises a glass substrate layer and a PDMS micro-flow channel layer positioned on the glass substrate layer; the glass substrate layer comprises a glass substrate and a plurality of pairs of microelectrodes arranged on the glass substrate layer; the PDMS micro-flow channel layer comprises a plurality of independent micro-fluidic channels; the microelectrodes on the glass substrate are corresponding to the micro-flow channels on the PDMS micro-flow channel layer one by one; the microelectrodes are electrically connected with an external circuit. The use method comprises the steps of cell capture, cell tissue culture, electrical impedance spectroscopy detection and tissue release. The chip disclosed by the invention is processed by a transparent substrate, microscopic imaging can be taken as a supplementary means of the electrical impedance spectroscopydetection, and the dynamic growth and the physiological behavior of cell tissues and other biological processes are subjected to observation and in-situ analysis. The micro-flow control chip can be used in the fields of biological research, drug screening and the like of cells or tissues.

The invention discloses a cell tissue culture system capable of accurately controlling air pressure. The cell tissue culture system comprises a culture room, a high pressure air source, an air flow meter, an air inlet pipeline, an air inlet switch, an air outlet pipeline, a pressure sensor, a decompression device, an exhaust valve and a control device, wherein the high pressure air source is communicated with an air inlet of the culture room through the decompression device, the air flow meter and the air inlet pipeline; the air inlet switch is arranged on the air inlet pipeline; an air outlet of the culture room is connected with the air outlet pipeline; the air outlet pipeline is communicated with the outside through the exhaust valve; the exhaust valve is used for controlling air in the culture room to be quantitatively updated; the air flow meter is used for measuring air intake flow in real time; the pressure sensor is used for sensing the air pressure in the culture room and on the air inlet pipeline and the air outlet pipeline; and the control device is used for controlling the air inlet switch to be turned on or off according to a signal from the pressure sensor. According to the cell tissue culture system, the air pressure in the culture room is stabilized to be in the preset level for a long time, and the air in the culture room is constantly updated to guarantee the accuracy of scientific research.

The invention discloses a method for improving the characteristics of wild broussonetia papyrifera through germ cell culture. The method comprises the steps of (1) taking wild broussonetia papyrifera as female parent; (2) selecting and slicing germ; (3) sterilizing the germ; (4) inducing callus; (5) promoting differentiation of the callus into seedling to obtain a complete plant; and (6) managing after differentiating into seedling. The method disclosed by the invention, on the basis of germ cell tissue culture technology, enhances fast growth performance of the wild broussonetia papyrifera, lengthens wood fiber and increases the content of plant protein in tender shoots and leaves, so that the wild broussonetia papyrifera can grow at ultra-fast speed and have long wood fiber, and the tender shoots and leaves are rich in nutrition.

It is an object of the present invention to provide a method for producing a collagen sponge and a method for producing an artificial skin which can give a high yield through simplified steps, no inferior goods being produced in the finally-obtained collagen sponges and artificial skins; and an artificial skin and a substrate for tissue engineering which respectively comprise a collagen sponge produced by the method for producing a collagen sponge. The present invention is a method for producing a collagen sponge, wherein a diluted collagen solution having a pH adjusted by use of acetic acid is used.

The invention relates to a method for the large-scale production of jatropha curcas plants by cell-tissue culture, belonging to the culture technology of plant seedlings. The method comprises the following steps: (1) cell suspension culture: grinding and inoculating jatropha curcas tissue and adding a cell suspension culture substrate: 0.2-0.5 mg/L of MS+BA, 1-6 mg/L of NAA, 0.5-1.5 mg/L of LH and 19-22 g/L of sucrose; (2) cell growth measurement; (3) clumpy bud multiplication culture: selecting cells growing into a resting stage to access a clumpy bud multiplication culture substrate: 0.1-1 mg/L of MS+6-BA, 0.1-1.5 mg/L and 20-30 g/L of sucrose; (4) rooting culture: selecting well-growing clumpy buds to access a rooting culture substrate: 0.1-1 mg/L of MS+NAA; and (5) bottling culture. The invention organically combines the cell culture and the tissue culture of jatropha curcas and has the advantages of short period and low cost, provides a high-efficiency path for manually culturing the jatropha curcas, producing virus-free seedlings and culturing fine varieties and has a favorable prospect for developing and utilizing the jatropha curcas.

Tissue culture medium such as porous frameworks and even open surface multidirectional porous frameworks may be used to provide uniform distribution of nourishment solutions, uniform interstitial voids as well as undistorted transport fields which may facilitate high volume yields of finished plants from cells, such as explants in a tissue culturing process. Further embodiments may include automating a tissue culturing process to reduce labor costs and increase uniformity of finished plants through tissue culture processes.

In order to detect the mis-operation of cells or tissues in a culturing step and other working steps to prevent erroneous use in treatment of cultured cell and tissues besides those of the cell donor, a genetic information, held by a cell or tissue, is used as a cell tissue information, which is inseparable from the cell tissue itself and enables individual identification of the cell donor, that is, identification of the origin of the cell or tissue subject to a culture process, and the cell tissue information before and after culturing are compared and collated.

The invention discloses a method for separating extracellular microcapsules from cells, tissue culture supernatant or body fluid, a specialized separating reagent, a kit and application. The method comprises the steps of alienating extracellular microcapsules in cells, tissue culture supernatant or body fluid from a water solution by virtue of a polyethylene glycol (PEG) solution, and concentrating and collecting the settle extracellular microcapsules by virtue of a low-speed centrifugation method. By virtue of the method, 1000mL or above specimens can be processed in each time; compared with ultracentrifugation and chromatography methods, the method has the advantages that the operation is simple, the elapsed time is short, large-sized instruments and equipment are not required, and the repeatability is good; and the problem that the extracellular microcapsules are difficult to separate is solved, and the scientific research and the clinical application of the extracellular microcapsules are promoted.

The invention relates to an anti-browning culture method of Haworthisa.spp cells. The method is characterized in that the method comprises the following steps: 1, inoculating the Haworthisa.spp cell culture into a sterile triangular flask, adding a culture solution into the triangular flask, putting the sterile triangular flask on a shaking table for proliferation suspension culture, taking the filtrate of the Haworthisa.spp cell culture obtained in the previous step, adding the culture solution, inoculating the material into the sterile triangular flask, and putting the sterile triangular flask on the shaking table for anti-browning suspension culture. The method directly takes the callus cells of the Haworthisa.spp as explants, firstly carries out suspension culture and then carries outsolid culture; meanwhile, the Himalaya glacier water is used for culturing the Haworthisa.spp cell tissue, and the glacier water can be used as an anti-browning agent, so that the browning phenomenonin the Haworthisa.spp cell tissue culture can be effectively prevented and solved, and can also be used as an additive to be added into a culture medium to promote proliferation of plant cell tissues,and scientific reference is provided for tissue culture and industrialization of the Haworthisa.spp cells.

The invention provides a device and method for in-vitro 3D culture of cells and tissue. The device includes a cover layer and a cell-tissue culture chamber layer, wherein the cover layer includes a liquid inlet hole, a liquid outlet hole, Luer joints are arranged on upper parts and lower parts of the liquid inlet hole and the liquid outlet hole, and the Luer joints can be externally connected to PE tubes; and the cell-tissue culture chamber layer includes multiple cell-tissue organ culture tanks, and buckle arms are arranged on both sides of each culture tank. When the device is applied to cell culture, multiple cell-tissue culture experiments can be performed in vitro at the same time, and the culture state of cells and tissue can be detected and monitored conveniently and quickly throughthe microfluidic design.

The invention relates to a health product and a preparation method thereof. The preparation method includes the steps A, inoculating Ganoderma sinensis to culture solution, and performing shake culture on a shaker to obtain fungus solution; B, inoculating the fungus solution obtained in the step A to liquid medium containing barley malt and soybean, and performing aerobic or anaerobic culture to obtain fermented Ganoderma sinensis mycelia and Ganoderma sinensis fermentation broth; C, subjecting the Ganoderma sinensis mycelia obtained in the step B to cell tissue culture breakage, and extracting with water to obtain Ganoderma sinensis extract; and D, mixing the Ganoderma sinensis fermentation broth obtained in the step B and the Ganoderma sinensis extract obtained in the step C, and diluting or concentrating obtained mixed liquor to obtain the Ganoderma sinensis health product. By the industrial submerged fermentation technology, the Ganoderma sinensis culture can be propagated industrially and massively in the liquid medium. Therefore, the process is simple, and the cost is low.

The invention relates to the technical field of cell tissue culture devices, and particularly relates to a negative pressure culture device convenient for collagen extraction. The negative pressure culture device comprises a shell, wherein the shell is connected with a cover plate; a partition plate is arranged in the shell; a sealing ring is arranged below the partition plate; a placement hole is formed in the partition plate; a bearing and a limiting rod are arranged on the bottom surface of the shell; the bearing is connected with a rotary column; and the rotary column is connected with a plugging block, a culture dish and a tray. A culture solution is added into a storage tank and reaches a liquid outlet through a conveying pipe and a hose, an electric push rod pushes the liquid outlet to the upper part of the culture dish, and the culture solution can be added into the culture dish under the condition that the internal space of the device is not opened, so that the device is more convenient and practical; and when the culture dish is taken and stored, only the rotary column needs to be rotated, so that the tray drives the culture dish to move upwards and jacks the plugging block open, the placement hole is always in a plugged state, the interior of the device can be still in a relatively closed state, the internal pressure cannot be greatly changed, and a good culture environment is kept.

A method for increasing the efficiency of homologous recombination-based gene editing in a plant according to an embodiment of the present invention includes optimizing temperature and photoperiod conditions during tissue culture of plant cells, expressing factors required for homology-directed DNA repair (HDR) and factors for increasing the HDR efficiency by using a multiple replicon, or regulating the HDR pathway or non-homologous end joining (NHEJ) pathway.

The invention particularly relates to a biological cell stimulation system based on a pneumatic annular mold, and a control method thereof. The biological cell stimulation system comprises an annular culture mold and air pump equipment; the annular culture mold is arranged in a culture dish and is connected with the air pump equipment through an air guide pipe; the annular culture mold comprises an upper mold, a lower mold and a mold base; the upper mold and the lower mold are in sealed connection, and the lower mold and the mold base are in sealed connection, so that the volume-variable annular culture mold is formed; the mold base is arranged in the culture dish; and a through hole is formed in the upper mold, and the air pump equipment is connected with the lower mold through an air guide pipe via the through hole. The system has no special requirements in cell tissue culture differentiation promotion application, does not need complex treatment on a cell sample, can realize integration of culture and stimulation, effectively reduces the damage and microbiological contamination probability of the cell tissue in the culture and stimulation process, and can be used for research on skeletal muscle tissue engineering, drug screening and life-like robots taking muscle cells as driving units.

The invention relates to the technical field of animal cell tissue culture, in particular to an animal cell tissue culture device and a culture method.The device comprises a culture platform, a connecting column, a culture frame, a cover plate, a shaking mechanism, a bearing disc, a culture dish and a feeding mechanism; a rotating disc drives a vertical moving block to move up and down on an annular adjusting plate in a reciprocating manner, so that the vertical moving block synchronously drives a bearing disc to shake in the process of driving the bearing disc to rotate through a shaking rod, and a nutrient solution in a culture dish is shaken and shaken up; in the feeding mechanism, according to the amount of nutrient solutions needed in all culture dishes, a valve is opened, air is extruded through an injection cylinder, the nutrient solutions are pumped into the culture dishes through cooperation of a liquid distribution pipe and a connecting pipe, a cover plate is not opened in the whole feeding process, and therefore the possibility that infectious microbes enter a cavity is reduced, and the feeding efficiency is improved. And the possibility of animal tissue cell culture polluted by infectious microbes is reduced.