Automatic liquid adding equipment for cell tissue culture

Abstract

The utility model relates to the technical field of cell tissue culture, and discloses automatic liquid adding equipment for cell tissue culture, which comprises a cell culture chamber, an operation panel, a culture chamber main chamber, a chamber door, a moving component, a liquid suction device, a solution bottle, a support plate and a culture dish, the cell culture chamber is hermetically fixed at the right part of the upper end of the supporting plate, the operation panel is obliquely arranged at the upper part of the culture chamber main chamber, the chamber door is arranged on the front side of the cell culture chamber in an openable manner, and the chamber door and the cell culture chamber are kept sealed; the moving assembly is fixed to the right side of the upper portion of the culture room main chamber, the liquid suction device is fixed to the driving end of the moving assembly, multiple rows of culture dishes and multiple solution bottles are arranged at the upper end of the supporting plate, and reagents needing to be added are stored in the solution bottles. The cell tissue culture device can guarantee the stability of a culture environment, improve the culture quality of cell tissues, reduce the working intensity of testers and facilitate flexible selection of culture schemes.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell tissue culture technical field especially, relates to a cell tissue culture is with automatic liquid adding equipment. BACKGROUND

[0002] When culturing cells, cell suspension is usually inoculated in a culture dish, and the culture is carried out in a CO2 cell culture box containing a certain amount of CO2 at a certain temperature. During the culture process, different culture media and / or nutrient solutions and / or other substances need to be added to provide water, carbohydrates, trace elements, vitamins, nitrogen-containing substances and other substances required for cell tissue growth and reproduction.

[0003] For continuous culture, all substances can be added to the culture medium at the beginning of the culture; but for segmented culture, substances need to be added during the process, which is usually manually added by the experimenter. This requires opening the cell culture box, which can cause changes in the culture environment in a short period of time, and can also introduce bacteria and other contaminants, affecting the subsequent culture quality of the cells. In addition, the experimenter needs to be present, increasing the work intensity of the experimenter, especially for long-term culture, which requires time allocation and is not convenient for flexible selection of culture schemes. SUMMARY

[0004] In order to overcome the deficiencies of the prior art, the purpose of the utility model is to provide an automatic liquid adding equipment for cell tissue culture, which can ensure the stability of the culture environment, improve the culture quality of the cell tissue, reduce the work intensity of the experimenter, and facilitate flexible selection of culture schemes.

[0005] The utility model adopts the following technical scheme to realize it: an automatic liquid adding equipment for cell tissue culture, comprising a cell culture chamber, an operation panel, a culture chamber main chamber, a chamber door, a moving assembly, a liquid suction device, a solution bottle, a support plate and a culture dish, the culture chamber main chamber is sealed and fixed on the upper end left part of the support plate, the cell culture chamber is sealed and fixed on the upper end right part of the support plate, the operation panel is inclinedly arranged on the upper part of the culture chamber main chamber, the chamber door is arranged on the front side of the cell culture chamber and can be opened and closed, and the chamber door and the cell culture chamber are kept sealed; the moving assembly is fixed on the upper right side of the culture chamber main chamber, the liquid suction device is fixed on the driving end of the moving assembly, the support plate upper end is provided with a plurality of rows of culture dishes and a plurality of solution bottles, the solution bottles store the reagents that need to be added, and the culture dishes inoculate the cell suspension to be cultured.

[0006] Further, the moving assembly comprises a horizontal moving structure, a longitudinal moving structure and a vertical moving structure, the horizontal moving structure is fixed on the upper right side of the culture chamber main chamber, the longitudinal moving structure is installed on the driving end of the horizontal moving structure, and the vertical moving structure is installed on the driving end of the longitudinal moving structure.

[0007] Further, the lateral movement structure, the longitudinal movement structure and the vertical movement structure are all screw sliding structures.

[0008] Further, the screw sliding structure comprises a connecting plate, support seats, a motor, a screw rod, a nut seat, sliding columns and pulleys, the support seats are fixed at two ends of the connecting plate, the motor is fixed outside one of the support seats, the screw rod is rotatably installed between the two support seats, one end of the screw rod is fixedly connected with a driving shaft of the motor, the sliding columns are symmetrically fixed at upper and lower sides of the connecting plate, the nut seat is connected with the screw rod through thread cooperation, the pulleys are fixed at upper and lower sides of the nut seat, and the pulleys are cooperatively installed on the sliding columns.

[0009] Further, the liquid suction device is provided with a liquid suction tube at a bottom end.

[0010] Further, the cell culture chamber and the chamber door are both made of transparent materials.

[0011] Compared with the prior art, the cell tissue culture automatic liquid adding equipment has the advantages that:

[0012] 1. The cell tissue culture automatic liquid adding equipment can realize automatic and segmented addition of substances, realizes automatic segmented culture, guarantees the stability of a culture environment, improves the culture quality of cell tissues, reduces the working intensity of test personnel, and is convenient for flexible selection of a culture scheme.

[0013] 2. The cell tissue culture automatic liquid adding equipment drives the screw rod to rotate by using the motor, drives the nut seat to move on the sliding columns through the pulleys at upper and lower sides of the nut seat, and thus drives the liquid suction device to move, and the moving precision is high. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 is a structural schematic view of the cell tissue culture automatic liquid adding equipment of the utility model;

[0015] Figure 2 is a structural schematic view of the cell tissue culture automatic liquid adding equipment of the utility model after removing the cell culture chamber and the chamber door;

[0016] Figure 3 is a connection structure schematic view of the moving assembly and the liquid suction device in the utility model;

[0017] Figure 4 is a structural schematic view of the screw sliding structure in the utility model.

[0018] In the figure: cell culture chamber-1; operation panel-2; culture chamber main chamber-3; chamber door-4; moving assembly-5; liquid suction device-6; solution bottle-7; support plate-8; culture dish-9; transverse moving structure-21; longitudinal moving structure-22; vertical moving structure-23; pipette-25; connecting plate-31; support seat-32; motor-33; screw rod-34; nut seat-35; sliding column-36; pulley-37. DETAILED DESCRIPTION

[0019] The implementation of the present application will be described below through specific examples, and other advantages and effects of the present application can be easily understood by those skilled in the art according to the disclosure. The present application can also be implemented or applied through different specific embodiments, and various modifications or changes can be made to the details in the specification without departing from the spirit of the present application. It should be noted that the following examples and features in the examples can be combined with each other without conflict.

[0020] The purpose of the present application is to overcome the defects of the prior art, and provide an automatic liquid adding equipment for cell tissue culture.

[0021] Example 1

[0022] The present application provides an automatic liquid adding equipment for cell tissue culture, which is referred to Figures 1-2As shown, it includes: cell culture chamber 1, operation panel 2, culture chamber main chamber 3, chamber door 4, moving assembly 5, liquid suction device 6, solution bottle 7, support plate 8 and culture dish 9, culture chamber main chamber 3 is sealed and fixed at the left upper end of support plate 8, cell culture chamber 1 is sealed and fixed at the right upper end of support plate 8, operation panel 2 is obliquely arranged at the upper part of culture chamber main chamber 3, and the culture chamber main chamber 3 in the embodiment uses the CO2 cell culture box in the prior art, which can provide a stable temperature and CO2 concentration environment, and the operation panel 2 is used for adjusting the environmental parameters in the culture chamber main chamber 3, and it should be noted that the control circuit and control mode of the operation panel 2 for controlling the culture chamber main chamber 3 are not particularly required in the utility model. The chamber door 4 is arranged on the front side of the cell culture chamber 1 and can be opened and closed, and the chamber door 4 and the cell culture chamber 1 are sealed; the cell culture chamber 1 and the chamber door 4 in the embodiment are both transparent plastic materials, and the inside of the cell culture chamber can be directly observed through the cell culture chamber 1 and the chamber door 4. A plurality of rows of culture dishes 9 and a plurality of solution bottles 7 are arranged on the upper end of the support plate 8, the solution bottles 7 store reagents to be added, the reagents can be carbohydrate, trace element, vitamin, nitrogen-containing substance and the like, and are used for cell tissue growth and reproduction, and the culture dishes 9 are inoculated with cell suspensions to be cultured. The moving assembly 5 is fixed on the right side of the upper part of the culture chamber main chamber 3, the liquid suction device 6 is fixed on the driving end of the moving assembly 5, and the liquid suction device 6 is provided with a suction tube 25 at the bottom end, and the liquid suction device 6 in the embodiment can use an electric pump assisted suction pipette or a pneumatic suction gun in the prior art, which is used for sucking and discharging solution from the solution bottle 7 through the suction tube 25.

[0023] Referring to Figure 3 As shown, the moving assembly 5 includes a horizontal moving structure 21, a longitudinal moving structure 22 and a vertical moving structure 23, the horizontal moving structure 21 is fixed on the right side of the upper part of the culture chamber main chamber 3, the longitudinal moving structure 22 is installed on the driving end of the horizontal moving structure 21, and the vertical moving structure 23 is installed on the driving end of the longitudinal moving structure 22. The horizontal moving structure 21, the longitudinal moving structure 22 and the vertical moving structure 23 in the embodiment are all screw sliding structures.

[0024] Specifically, referring to Figure 4 As shown, the screw sliding structure includes a connecting plate 31, a support seat 32, a motor 33, a screw 34, a nut seat 35, a sliding column 36 and a pulley 37, the support seat 32 is fixed at both ends of the connecting plate 31, the motor 33 is fixed outside one of the support seats 32, the screw 34 is rotatably installed between the two support seats 32, one end of the screw 34 is connected and fixed with the driving shaft of the motor 33, the sliding column 36 is symmetrically fixed on the upper and lower sides of the connecting plate 31, the nut seat 35 is connected with the screw 34 through thread cooperation, the pulley 37 is fixed on the upper and lower sides of the nut seat 35, and the pulley 37 is cooperatively installed on the sliding column 36.

[0025] The utility model discloses a kind of automatic liquid adding equipment for cell tissue culture, when using, trace element, vitamin, nitrogen-containing substance etc. for the substance solution required for cell tissue growth and propagation, test are stored in solution bottle 7, cell suspension to be cultivated is inoculated in culture dish 9, then close room door 4, adjust the stable temperature and CO2 concentration environment provided in incubation chamber main chamber 3, set the kind and time of reagent addition. Reach preset time, moving assembly 5 utilizes motor drive screw 34 rotation, drive nut seat 35 to move on slide column 36 by its upper and lower two sides' pulley 37, so that liquid suction device 6 reaches the above of solution bottle 7, utilize pipette 25 to suck reagent from solution bottle 7, then move and add in culture dish 9 where reagent needs to be added, and moving precision is high. To add material automatically, segmented, realize automatic segmented culture, in this process of adding material, experimental personnel need not to be on duty, manually add and open room door 4, so as to can guarantee the stability of culture environment, improve the culture quality of cell tissue, reduce the work intensity of test personnel, it is convenient to flexibly select culture scheme.

[0026] In addition, it should be understood that, although the present specification is described according to the embodiments, not every embodiment contains only one independent technical solution, the description manner of the specification is only for clarity, the skilled in the art should take the specification as a whole, the technical solution in each embodiment can also be combined appropriately, form other embodiments that the skilled in the art can understand.

Claims

1. An automated liquid dispensing device for cell and tissue culture, characterized in that, include: The cell culture chamber (1), operation panel (2), main chamber (3), door (4), moving assembly (5), aspirator (6), solution bottle (7), support plate (8), and culture dish (9) are provided. The main chamber (3) is sealed and fixed to the upper left side of the support plate (8), and the cell culture chamber (1) is sealed and fixed to the upper right side of the support plate (8). The operation panel (2) is inclined and set on the upper part of the main chamber (3). The door (4) is set on the front side of the cell culture chamber (1) and the door (4) is sealed with the cell culture chamber (1). The moving assembly (5) is fixed on the upper right side of the main chamber (3). The aspirator (6) is fixed on the driving end of the moving assembly (5). Several rows of culture dishes (9) and several solution bottles (7) are provided on the upper end of the support plate (8). The solution bottles (7) store the reagents to be added. The culture dishes (9) are inoculated with cell suspension to be cultured.

2. The automatic liquid dispensing device for cell and tissue culture according to claim 1, characterized in that: The moving component (5) includes a horizontal moving structure (21), a vertical moving structure (22), and a vertical moving structure (23). The horizontal moving structure (21) is fixed on the upper right side of the main chamber (3) of the culture chamber. The vertical moving structure (22) is installed at the driving end of the horizontal moving structure (21), and the vertical moving structure (23) is installed at the driving end of the vertical moving structure (22).

3. The automatic liquid dispensing device for cell and tissue culture according to claim 2, characterized in that: The horizontal moving structure (21), the vertical moving structure (22), and the vertical moving structure (23) are all lead screw sliding structures.

4. The automatic liquid dispensing device for cell and tissue culture according to claim 3, characterized in that: The aforementioned lead screw sliding structure includes a connecting plate (31), a support base (32), a motor (33), a lead screw (34), a nut seat (35), a sliding column (36), and a pulley (37). The support base (32) is fixed at both ends of the connecting plate (31). The motor (33) is fixed on the outside of one of the support bases (32). The lead screw (34) is rotatably installed between the two support bases (32). One end of the lead screw (34) is connected and fixed to the drive shaft of the motor (33). The sliding column (36) is symmetrically fixed on the upper and lower sides of the connecting plate (31). The nut seat (35) is connected to the lead screw (34) through a threaded connection. The pulley (37) is fixed on the upper and lower sides of the nut seat (35). The pulley (37) is fitted onto the sliding column (36).

5. The automatic liquid dispensing device for cell and tissue culture according to claim 1, characterized in that: The suction tube (25) is installed at the bottom of the suction device (6).

6. The automatic liquid dispensing device for cell and tissue culture according to claim 1, characterized in that: The cell culture chamber (1) and the chamber door (4) are both made of transparent material.

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