A cultivation experimental device for studying the interaction between plant ectomycorrhizal fungi

Abstract

This invention discloses a cultivation experimental device for studying plant ectomycorrhizal fungal interactions. It includes a base with a petri dish on it. A protective shell is installed around the petri dish on the top of the base. A circular plate is rotatably mounted inside the protective shell. Both the circular plate and the top surface of the protective shell have two symmetrical arc-shaped ventilation openings. An adjustment component connects the circular plate and the protective shell, including a splined sleeve rotatably connected to the top of the protective shell. This invention provides a cultivation experimental device for studying plant ectomycorrhizal fungal interactions that offers high adjustment precision, effortless operation, and stability. The precise design of the ventilation opening adjustment component allows for accurate control of the opening size, meeting the oxygen requirements of different fungal species and plants. Furthermore, the threaded connection of the protective shell enhances the stability and practicality of the device, providing strong support for scientific research.

Description

Technical Field

[0001] This utility model relates to the field of cultivation technology, and in particular to a cultivation experimental device for studying the interaction between plant ectomycorrhizal fungi. Background Technology

[0002] A close symbiotic relationship exists between plants and ectomycorrhizal fungi. Ectomycorrhizal fungi can infect plant roots, forming mycorrhizal symbionts. This symbiotic relationship is of great significance to plant growth and development. Ectomycorrhizal fungi expand the absorption range of plant roots through their hyphal networks, helping plants absorb water and minerals from the soil, especially key nutrients such as phosphorus and nitrogen. At the same time, plants also provide ectomycorrhizal fungi with necessary carbohydrates and other nutrients to maintain their life activities. With the continuous development of science and technology, people's understanding of the interaction mechanism between plants and ectomycorrhizal fungi has gradually deepened. However, to further reveal the inner mechanism of this interaction, more precise and systematic scientific research is needed, such as conducting cultivation experiments and using petri dishes with protective shells to culture and study plants and ectomycorrhizal fungi.

[0003] In the cultivation experiment, the vents play a crucial role in the cultivation experimental device. They are generally set on the shell and allow air circulation to provide the necessary oxygen for plants and ectomycorrhizal fungi. When conducting research on plant-ectomycorrhizal fungi interactions, the size of the vents needs to be determined according to the specific experimental purpose and the types of plants and fungi. For example, for fungal species that require a high humidity environment, smaller vents can be selected, while for plant species that require sufficient oxygen, larger vents can be selected.

[0004] To improve applicability, most existing cultivation devices are designed with adjustable vent sizes. For example, a wild fungus mycorrhizal symbiotic cultivation device (patent number CN213939147U) uses a baffle, a pressing block, and a pull rod. Dragging the pull rod moves the baffle to the right, preventing it from blocking the vents. The pressing block engages with the baffle under spring pressure, limiting its position and allowing ventilation. This enables the cultivation device to meet the environmental requirements for wild fungus mycorrhizal symbiotic cultivation. This design offers ventilation and adjustable vent sizes. However, this design still has shortcomings. The adjustment precision is low, allowing only two adjustment ranges, limiting applicability. Secondly, adjustment is laborious, requiring overcoming the elasticity of multiple springs, making operation inconvenient. Finally, the outer frame is not stable when placed directly, easily shifting or even falling.

[0005] Therefore, it is necessary to propose a cultivation experimental device for studying the interaction between plant ectomycorrhizal fungi to solve this problem. Utility Model Content

[0006] This invention provides a cultivation experimental device for studying the interaction between plant ectomycorrhizal fungi, which solves the problems mentioned in the background.

[0007] To solve the above-mentioned technical problems, this utility model provides a cultivation experimental device for studying the interaction of plant ectomycorrhizal fungi, including a device base, a petri dish on the device base, and a protective shell around the petri dish on the top of the device base. A circular plate is rotatably mounted inside the top surface of the protective shell. The circular plate and the top surface of the protective shell each have two symmetrical arc-shaped ventilation holes. An adjustment component is connected between the circular plate and the protective shell. The adjustment component includes a spline sleeve rotatably connected to the top of the protective shell. The spline sleeve shares a rotating shaft with the circular plate and a spline shaft is inserted into its top. The spline shaft is fixed to the bottom of the rotating plate. Positioning rods are symmetrically arranged at both ends of the bottom of the rotating plate. The positioning rods can be inserted into rod holes arranged in a ring on the top of a positioning ring plate. The rod holes are arranged in a ring. The positioning ring plate is fixed to the top of the protective shell.

[0008] Preferably, guide rods are symmetrically arranged at both ends of the top of the rotating plate, and multiple counterweights can be stacked on the top of the rotating plate, with the guide rods passing through the counterweights.

[0009] Preferably, the positioning rod has ring-shaped holes at its top that can be fitted with rubber plugs for sealing and dust prevention.

[0010] Preferably, the bottom ends of the rotating plate are symmetrically fixed with retaining frames, and the sides of the spline sleeve are symmetrically fixed with retaining blocks, the retaining blocks passing through the openings of the retaining frames.

[0011] Preferably, the inner wall of the arc-shaped vent of the protective shell is equipped with an arc-shaped filter screen, and the top surface of the filter screen is designed to be flush with the top surface of the protective shell.

[0012] Preferably, the outer wall of the protective shell is integrally provided with a slot, into which an information plate is inserted from the top.

[0013] Preferably, the top of the device base is provided with an internal threaded groove around the petri dish, and the internal threaded groove and the external thread at the lower end of the protective shell form a threaded connection.

[0014] Compared with related technologies, the cultivation experimental device for studying plant ectomycorrhizal fungi interactions provided by this utility model has the following beneficial effects:

[0015] This invention provides a cultivation experimental device for studying plant ectomycorrhizal fungal interactions, which features high adjustment precision, labor-saving operation, and stability. Through the design of a finely crafted air vent adjustment component, the size of the air vent is precisely controlled, meeting the oxygen requirements of different fungal species and plants. Furthermore, the threaded connection of the protective shell enhances the stability and practicality of the device, providing strong support for scientific research. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall three-dimensional structure of this utility model;

[0017] Figure 2 This is a three-dimensional structural diagram of the device base of this utility model;

[0018] Figure 3 This is a cross-sectional perspective view of the protective shell of this utility model.

[0019] Figure 4 This is a schematic diagram of the three-dimensional structure of the adjustment component of this utility model. Figure 1 ;

[0020] Figure 5 This is a schematic diagram of the three-dimensional structure of the adjustment component of this utility model. Figure 2 ;

[0021] Figure 6 For the present utility model Figure 3 A magnified structural diagram at point A in the diagram.

[0022] The following are the labeling elements in the diagram: 1. Device base; 11. Petri dish; 12. Internal threaded groove; 2. Protective shell; 21. Arc-shaped vent; 22. Circular plate; 23. Filter screen; 24. Slot; 25. Information plate; 3. Adjustment component; 31. Splined sleeve; 32. Splined shaft; 33. Rotating plate; 34. Positioning rod; 35. Positioning ring plate; 36. Guide rod; 37. Counterweight; 38. Rubber stopper; 39. Locking block; 310. Locking frame. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0024] Depend on Figures 1-6The present invention includes a device base 1, on which a petri dish 11 is mounted. A protective shell 2 is provided on the top of the device base 1 around the petri dish 11. A circular plate 22 is rotatably mounted on the inner top surface of the protective shell 2. The top surfaces of the circular plate 22 and the protective shell 2 are each provided with two symmetrical arc-shaped vents 21. An adjustment assembly 3 is connected between the circular plate 22 and the protective shell 2. The adjustment assembly 3 includes a spline sleeve 31 rotatably connected to the top of the protective shell 2. The spline sleeve 31 shares a rotating shaft with the circular plate 22 and a spline shaft 32 is inserted into its top. The spline shaft 32 is fixed to the bottom of a rotating plate 33. A handle is provided on each side of the rotating plate 33. Positioning rods 34 are symmetrically provided at both ends of the bottom of the rotating plate 33. The positioning rods 34 can be inserted into rod holes provided on the top of a positioning ring plate 35. The rod holes are arranged in a ring. The positioning ring plate 35 is fixed to the top of the protective shell 2.

[0025] By adjusting the design of component 3, circular plate 22, and arc-shaped vent 21, during the cultivation experiment for studying the interaction between plant ectomycorrhizal fungi, soil is placed in petri dish 11, and the fungal inoculum and plant are placed together in the soil of petri dish 11. Then, the protective shell 2 is installed. At this time, the circular plate 22 and the arc-shaped vent 21 of the protective shell 2 are completely misaligned, and the positioning rod 34 is inserted into the circular hole of the positioning ring plate 35. Therefore, the protective shell 2 cannot be ventilated, and the circular plate 22 cannot rotate. Finally, when the vent is opened according to the characteristics of the fungal inoculum and plant, the handles on both sides of the rotating plate 33 are held and pulled upwards. The spline shaft 32 will also move upwards but will still be in the spline sleeve 31. At this time, the rotating plate 33 is driven to rotate, so that the spline shaft 32... The rotating plate 33 rotates the spline sleeve 31, which in turn rotates the circular plate 22, causing the upper and lower arc-shaped vents 21 to partially overlap. This allows air to enter the protective shell 2 through the overlapping vents, providing the necessary oxygen and other gas exchange for the bacteria and plants in the culture dish 11. The degree of overlap of the arc-shaped vents 21 can be precisely controlled by the continuous rotation of the rotating plate 33, thereby adjusting the gas flow rate into the protective shell 2. When the required level of ventilation is reached, the rotating plate 33 is slowly released, and the positioning rod 34 will fall back into the corresponding rod hole on the positioning ring plate 35, achieving a stable fixation of the current ventilation state. This design offers a wider range of adjustment, better adaptability, and is very labor-saving and convenient.

[0026] Guide rods 36 are symmetrically arranged at both ends of the top of the rotating plate 33. Multiple counterweights 37 can be stacked on the top of the rotating plate 33. The counterweights 37 can be passed through by the guide rods 36. The counterweights 37 located at the top are only inserted by the guide rods 36, not passed through. The top of the positioning rods 34 has rod holes arranged in a ring, into which rubber plugs 38 can be inserted for sealing and dust prevention. The bottom ends of the rotating plate 33 are symmetrically fixed with clip frames 310. The spline sleeve 31 is symmetrically fixed with clip blocks 39 on both sides. The clip blocks 39 pass through the frame opening of the clip frame 310.

[0027] In the further optimized design of the adjustment component 3, after adjustment, a counterweight 37 can be placed to further improve stability. The guide rod 36 not only provides stable support and guidance for the counterweight 37, but also ensures the balance and safety of the counterweight 37 during stacking. Note that the counterweight 37 should be removed during adjustment. After adjustment, multiple rubber plugs 38 should be inserted into the empty rod holes to effectively prevent dust, impurities, etc. from entering the rod holes. Finally, the locking block 39 cooperates with the locking frame 310 to prevent loosening or falling off during rotation, which limits the maximum upward distance of the rotating plate 33 and satisfies the requirement that the positioning rod 34 disengage from the rod hole of the positioning ring plate 35, further enhancing the connection stability and reliability between the rotating plate 33 and the spline sleeve 31.

[0028] The inner wall of the arc-shaped vent 21 of the protective shell 2 is equipped with an arc-shaped filter screen 23. The top surface of the filter screen 23 is aligned with the top surface of the protective shell 2. The outer wall of the protective shell 2 is integrally provided with a slot 24. An information plate 25 is inserted into the slot 24 from the top. The top of the device base 1 is provided with an internal thread groove 12 around the petri dish 11. The internal thread groove 12 and the external thread at the lower end of the protective shell 2 form a threaded connection.

[0029] Further optimization of the protective shell 2: The filter 23 installed on the inner wall of the arc-shaped vent 21 not only effectively blocks large particles of impurities and dust from entering the interior of the protective shell 2, protecting the microorganisms and plants from contamination, but its arc-shaped design also perfectly matches the shape of the vent, ensuring smooth airflow and preventing unnecessary interference with the internal environment. Furthermore, the design of the top surface of the filter 23 being flush with the top surface of the protective shell 2 makes the entire device look cleaner and more aesthetically pleasing. Secondly, the combination design of the slot 24 and the information plate 25 provides researchers with convenient labeling and recording space. Researchers can record important information such as experimental information, microbial names, and planting dates on the information plate 25 and then insert it into the slot 24 for easy viewing and recording. Finally, the internal threaded groove 12 on the top of the device base 1 forms a threaded connection with the external thread at the bottom of the protective shell 2. This design makes the installation and removal of the protective shell 2 more convenient and quick, and also ensures a tight connection between it and the device base 1, preventing air leakage or loosening during the experiment.

[0030] Working principle: In the cultivation experiment for studying the interaction between plant ectomycorrhizal fungi, soil is placed in the culture dish 11, and the fungal inoculum and plant are placed together in the soil of the culture dish 11. Then, the internal threaded groove 12 is connected and installed with the protective shell 2. Then, important information such as experimental information, fungal name, and planting date are recorded on the information plate 25 and then inserted into the slot 24. When setting the vent, hold the handles on both sides of the rotating plate 33 and pull it up. The spline shaft 32 will also move up but will still be in the spline sleeve 31. At this time, drive the rotating plate 33 to rotate, so that the spline shaft 32 drives the spline sleeve 31 to rotate. Then, the circular plate 22 can be rotated, so that the upper and lower arc-shaped vents 21 overlap, thereby allowing air to enter the interior of the protective shell 2 through the overlapping vents, providing the necessary oxygen and other gas exchange for the fungal inoculum and plant in the culture dish 11. Finally, slowly release the rotating plate 33, and the positioning rod 34 will fall back into the corresponding rod hole on the positioning ring plate 35.

[0031] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0032] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cultivation experimental device for studying the interaction of plant ectomycorrhizal fungi, comprising a device base (1), a petri dish (11) on the device base (1), and a protective shell (2) surrounding the petri dish (11) on the top of the device base (1), characterized in that: The protective shell (2) has a circular plate (22) mounted on its inner top surface. The circular plate (22) and the protective shell (2) each have two symmetrical arc-shaped vents (21) on their top surfaces. An adjustment assembly (3) is connected between the circular plate (22) and the protective shell (2). The adjustment assembly (3) includes a spline sleeve (31) rotatably connected to the top of the protective shell (2). The spline sleeve (31) shares a rotating shaft with the circular plate (22) and a spline shaft (32) is inserted into the top. The spline shaft (32) is fixed to the bottom of the rotating plate (33). Positioning rods (34) are symmetrically arranged at both ends of the bottom of the rotating plate (33). The positioning rods (34) can be inserted into the rod holes provided on the top of the positioning ring plate (35). The rod holes are arranged in a ring. The positioning ring plate (35) is fixed to the top of the protective shell (2).

2. The cultivation experimental apparatus for studying plant ectomycorrhizal fungi interactions according to claim 1, characterized in that, The rotating plate (33) has guide rods (36) symmetrically arranged at both ends of the top. Multiple counterweights (37) can be stacked on the top of the rotating plate (33), and the counterweights (37) can be passed through by the guide rods (36).

3. The cultivation experimental apparatus for studying plant ectomycorrhizal fungi interactions according to claim 2, characterized in that, The positioning rod (34) has ring-shaped holes at the top that can be filled with rubber plugs (38) for sealing and dust prevention.

4. The cultivation experimental apparatus for studying plant ectomycorrhizal fungi interactions according to claim 3, characterized in that, The rotating plate (33) has symmetrically fixed frames (310) at both ends of its bottom, and the spline sleeve (31) has symmetrically fixed blocks (39) on both sides, with the blocks (39) passing through the opening of the frames (310).

5. The cultivation experimental apparatus for studying plant ectomycorrhizal fungi interactions according to claim 1, characterized in that, The inner wall of the arc-shaped vent (21) of the protective shell (2) is equipped with an arc-shaped filter screen (23), and the top surface of the filter screen (23) is designed to be flush with the top surface of the protective shell (2).

6. The cultivation experimental apparatus for studying plant ectomycorrhizal fungi interactions according to claim 5, characterized in that, The outer wall of the protective shell (2) is integrally provided with a slot (24), and an introduction plate (25) is inserted into the slot (24) from the top.

7. The cultivation experimental apparatus for studying plant ectomycorrhizal fungi interactions according to claim 5, characterized in that, The device base (1) has an internal thread groove (12) on the top of the petri dish (11) and the internal thread groove (12) is threaded to the external thread at the lower end of the protective shell (2).

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