Biomechanical system for cell tissue culture and biological material preparation

Abstract

The invention relates to the technical field of biology, in particular to a biomechanical system for cell tissue culture and biological material preparation, which comprises a reactor module, a power pressurization module, a monitoring sensor, a computer and a circulation module, the reactor module is arranged on the power pressurization module, the monitoring sensor is connected with the power pressurization module, and the computer is connected with the circulation module. The computer is connected with the monitoring sensor; the circulating module is connected with the sensor and the reactor module; the power pressurization module comprises a rack, a driving assembly, a screw and a pressure sensor, the screw and the driving assembly are installed on the rack, the driving assembly drives the pressure sensor to move through the screw, and in this way, the problems that in the prior art, a biomechanical device is too large in size, too heavy in mass, high in use cost and poor in practicability due to equipment integration are solved. The mechanical control precision is poor, and the pressurizing effect is single.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, and more particularly to a biomechanical system for cell and tissue culture and biomaterial preparation. Background Technology

[0002] In today's booming biotechnology landscape, cell and tissue culture and biomaterial preparation have become key forces driving progress in life sciences and numerous practical applications. Cell and tissue culture, as one of the fundamental technologies in life science research, provides an indispensable research model for in-depth exploration of cellular physiological characteristics, molecular mechanisms, and the development of diseases. Biomaterial preparation serves as a bridge connecting biotechnology with clinical medicine, tissue engineering, and other fields. Biomaterials play a crucial role in repairing or replacing damaged tissues and organs, drug delivery systems, and biosensors.

[0003] Existing patent publication number CN201737949U discloses a cell culture device for periodically switching between positive and negative pressure, including a pressurizing device, a depressurizing device, an air pressure sensor, an air pressure control unit, and a semi-permeable membrane. A culture medium cavity is formed between the semi-permeable membrane and the bottom surface of the culture chamber. In use, culture medium is injected into the culture medium cavity, and then cells are implanted onto the semi-permeable membrane. After activating the pressure regulating component, the air pressure sensor detects the air pressure inside the culture chamber and transmits the signal to the air pressure control unit. When the air pressure in the culture chamber reaches a predetermined positive or negative pressure value, the control unit sends a depressurization or pressurization command to the depressurization or pressurization control valve. Through depressurization and pressurization operations, the periodic switching between positive and negative pressure is achieved. While this device can achieve positive and negative pressure conversion to some extent, the pressure values ​​it can reach are relatively small, and it cannot even simulate the pressure of some parts of the human body that are subject to greater stress, such as intervertebral discs and lower limb articular cartilage. Because the cells are seeded on a semi-permeable membrane, the membrane will deform during changes in air pressure, generating tension or shear force. Both tension and shear force will affect the cells, so it is impossible to determine whether the final result is due to the effect of tension and shear force or the change in air pressure. At the same time, the overall design of this device has high requirements for airtightness, making it prone to failure during actual operation. Furthermore, because of the presence of the air compressor, the imaging volume is relatively large during operation, and the whole device appears rather bulky and cumbersome.

[0004] Patent publication number CN111855423A discloses a bioreactor for cartilage production, used to test cartilage samples. It includes a sterile incubator, a power cylinder, and a rotary servo motor. The sterile incubator has a cavity containing a test box with an opening at the top. The power cylinder includes a cylinder body and a piston rod. The cylinder body is positioned above the sterile incubator, with one end of the piston rod extending into the cylinder body and the other end extending into the cavity and connected to a pressure plate. The pressure plate seals the opening of the test box, and the cartilage sample is placed in the test box. A partition is provided within the cavity, with the test box positioned above the partition. The rotary servo motor is fixed below the partition, and its drive shaft passes through the partition and is fixed to the test box. The power cylinder can test the vertical force on the cartilage sample, while the rotary servo motor can quickly test the shear force. Simultaneously, the test liquid is mixed and stirred during the test. However, this equipment can only be used to directly pressurize the target material and cannot meet the needs of target materials requiring hydrostatic pressure. Furthermore, this equipment cannot be used for cell or tissue engineering culture. All components of the device are in one unit, making it difficult to move and use.

[0005] Patent publication number CN101397539A discloses a force-applying device for simulating human physiological stress in biomimetic culture of tissue-engineered tissues. The device includes a support base, on which a linear servo motor and a culture chamber are mounted. A switch contact and a tension / compression sensor are mounted at one end of the linear servo motor's shaft. A limit switch is mounted on the bushing of the servo motor shaft. The tension / compression sensor is connected to a connector via a helical spring. A guide shaft is provided on the tension / compression sensor, passing through the helical spring and inserted into the connector, slidingly engaging with it. The guide shaft has axial displacement freedom within the connector. The culture chamber is equipped with a device for applying stress to the cultured tissue. The rod of this stress-applying device extends out of the culture chamber and connects to the connector, slidingly engaging with it. This device can simulate physiological stress for the culture and preparation of tissue-engineered bone, cartilage, or tendons, achieving biomimetic culture of tissue-engineered bone, cartilage, or tendons. However, the mechanical force provided by this device is limited. Furthermore, the manufacturing cost of the equipment is extremely high, and its large size and heavy weight make it inconvenient to move and transport.

[0006] In summary, existing biomechanical devices suffer from problems such as excessive size, weight, and operating costs due to integrated equipment, as well as poor mechanical control precision and limited pressurization effect. Summary of the Invention

[0007] The purpose of this invention is to provide a biomechanical system for cell tissue culture and biomaterial preparation, aiming to solve the technical problems of existing biomechanical devices, such as excessive size, excessive weight, high cost due to integrated equipment, poor mechanical control precision, and limited pressure application effect.

[0008] To achieve the above objectives, the present invention employs a biomechanical system for cell tissue culture and biomaterial preparation, comprising a reactor module, a power pressurization module, a monitoring sensor, a computer, and a circulation module. The reactor module is mounted on the power pressurization module, the monitoring sensor is connected to the power pressurization module, the computer is connected to the monitoring sensor, and the circulation module is connected to both the sensor and the reactor module.

[0009] The power pressurization module includes a frame, a drive assembly, a screw, and a pressure sensor. The screw and the drive assembly are mounted on the frame, and the drive assembly drives the pressure sensor to move via the screw.

[0010] The drive assembly includes a servo motor, a drive wheel, a driven wheel, a belt, and a transmission component. The transmission component is rotatably connected to the frame, and the screw is threadedly engaged with the transmission component. The drive wheel and the driven wheel are fixedly connected to the output end of the servo motor and the transmission component, respectively, and the belt is disposed between the drive wheel and the driven wheel.

[0011] The reactor module includes a metal sleeve, a support member, a metal shaft, and a piston. The metal sleeve is mounted on the frame, the support member is placed inside the metal sleeve, the piston is slidably connected to the support member, and the piston is fixedly connected to the metal shaft.

[0012] The metal sleeve has an opening for connecting the carrier to the monitoring sensor and the circulation module.

[0013] The reactor module further includes a spiral cap, which is threadedly connected to the carrier.

[0014] The adjustable accuracy of the servo motor is 0.01 mm, and the accuracy of the pressure sensor is 0.01 kg, or 0.1 N.

[0015] This invention discloses a biomechanical system for cell and tissue culture and biomaterial preparation. In practical use, cells, tissue-engineered tissues, and biomaterial raw materials are placed into the tube body, and the front end of the tube body is sealed with a spiral cap, making the carrier a bioreactor capable of being pressurized at any time and withstanding a pressure of not less than 4 MPa. Subsequently, the carrier is placed in the metal sleeve, and the drive assembly is activated. The drive assembly drives the active wheel to rotate, and the active wheel drives the driven wheel to rotate via the belt. The driven wheel drives the transmission component to rotate, which in turn drives the screw to move. The movement of the screw simultaneously drives the pressure sensor to move, and the pressure sensor drives the metal shaft to move, causing the piston to slide within the tube body to achieve pressurization. At the same time, the monitoring sensor is used to monitor the internal hydrostatic pressure (or pressure intensity), oxygen partial pressure, carbon dioxide partial pressure, pH value, glucose, and lactic acid within the tube body, and circulation is achieved through the circulation module. This method solves the technical problems of existing biomechanical devices, such as excessive size, excessive weight, high operating cost due to integrated equipment, poor mechanical control accuracy, and single pressurization effect. Attached Figure Description

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0017] Figure 1 This is a schematic diagram of the biomechanical system of the present invention for cell tissue culture and biomaterial preparation.

[0018] Figure 2 This is a partial structural schematic diagram of the biomechanical system for cell tissue culture and biomaterial preparation according to the present invention.

[0019] Figure 3 This is a partial structural schematic diagram of the biomechanical system of the present invention for cell tissue culture and biomaterial preparation.

[0020] Figure 4 This is a schematic diagram showing the separation of the tube body and piston in this invention.

[0021] 1-Reactor module, 2-Power pressurization module, 3-Monitoring sensor, 4-Computer, 5-Circulation module, 6-Frame, 7-Screw, 8-Pressure sensor, 9-Servo motor, 10-Driving wheel, 11-Driven wheel, 12-Belt, 13-Transmission component, 14-Metal sleeve, 15-Metal shaft, 16-Piston, 17-Pipe body, 18-Screw cap, 19-Opening, 20-Heat vent, 21-Filter screen, 22-Programmable interface, 23-Self-adjusting module. Detailed Implementation

[0022] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, but should not be construed as limiting the present invention.

[0023] Please see Figures 1 to 4 , Figure 1 This is a schematic diagram of the biomechanical system of the present invention for cell tissue culture and biomaterial preparation. Figure 2 This is a partial structural schematic diagram of the biomechanical system for cell tissue culture and biomaterial preparation according to the present invention. Figure 3 This is a partial structural schematic diagram of the biomechanical system of the present invention for cell tissue culture and biomaterial preparation. Figure 4 This is a schematic diagram showing the separation of the tube body and piston in this invention.

[0024] This invention provides a biomechanical system for cell tissue culture and biomaterial preparation, comprising a reactor module 1, a power pressurization module 2, a monitoring sensor 3, a computer 4, and a circulation module 5. The reactor module 1 is mounted on the power pressurization module 2, the monitoring sensor 3 is connected to the power pressurization module 2, the computer 4 is connected to the monitoring sensor 3, and the circulation module 5 is connected to both the sensor and the reactor module 1.

[0025] The power pressurization module 2 includes a frame 6, a drive assembly, a screw 7, and a pressure sensor 8. The screw 7 and the drive assembly are mounted on the frame 6, and the drive assembly drives the pressure sensor 8 to move through the screw 7.

[0026] The drive assembly includes a servo motor 9, a drive wheel 10, a driven wheel 11, a belt 12, and a transmission component 13. The transmission component 13 is rotatably connected to the frame 6. The screw 7 is threadedly engaged with the transmission component 13. The drive wheel 10 and the driven wheel 11 are fixedly connected to the output end of the servo motor 9 and the transmission component 13, respectively. The belt 12 is disposed between the drive wheel 10 and the driven wheel 11.

[0027] The reactor module 1 includes a metal sleeve 14, a support member, a metal shaft 15, and a piston 16. The metal sleeve 14 is mounted on the frame 6. The support member is placed inside the metal sleeve 14. The piston 16 is slidably connected to the support member and fixedly connected to the metal shaft 15. After assembly, the support member, the metal shaft 15, and the piston 16 form a syringe shape.

[0028] The support component includes a tube body 17 and a spiral cap 18. The tube body 17 is placed inside the metal sleeve 14, and the spiral cap 18 is threadedly connected to the tube body 17.

[0029] The adjustable accuracy of the servo motor 9 is 0.01 mm, and the accuracy of the pressure sensor 8 is 0.01 kg, or 0.1 N.

[0030] In this specific embodiment, during use, cells, tissue-engineered tissues, and biomaterial raw materials are placed into the tube 17, and the front end of the tube 17 is sealed with the spiral cap 18, making the carrier a bioreactor capable of withstanding pressures of not less than 4 MPa. The carrier is then placed inside the metal sleeve 14, and the drive assembly is activated. The drive assembly drives the drive wheel 10 to rotate, which in turn drives the driven wheel 11 to rotate via the belt 12. The driven wheel 11 then drives the transmission component 13 to rotate, which in turn drives the screw 7. The screw 7 moves, driving the pressure sensor 8 to move as well. The pressure sensor 8 drives the metal shaft 15 to move, causing the piston 16 to slide within the tube 17 to apply pressure. Simultaneously, the monitoring sensor 3 monitors the internal hydrostatic pressure (or pressure intensity), oxygen partial pressure, carbon dioxide partial pressure, pH value, glucose, and lactic acid within the tube 17. Circulation is achieved through the circulation module 5. This method solves the technical problems of existing biomechanical devices, such as excessive size, weight, and high operating costs due to integrated equipment, poor mechanical control accuracy, and limited pressure application.

[0031] The metal sleeve rod is provided with an opening 19, which is used to connect the bearing member to the monitoring sensor 3 and the circulation module 5.

[0032] In this specific embodiment, during actual use, by providing the opening 19, the tube body 17 can be easily connected to the monitoring sensor 3 and the circulation module 5.

[0033] Meanwhile, the power pressurization module 2 also includes a heat dissipation port 20, and a filter screen 21 is provided at the heat dissipation port 20.

[0034] In this specific embodiment, during actual use, the heat dissipation vent 20 is used to dissipate the heat generated by the internal components of the frame 6, and the filter 21 is used to prevent dust and other impurities from entering the frame 6.

[0035] Secondly, the biomechanical system for cell tissue culture and biomaterial preparation includes a programmable interface 22, which is connected to the power pressurization module 2.

[0036] In this specific embodiment, by setting the programmable interface 22, different parameters can be set according to different requirements. It can not only continuously pressurize, but also pressurize back and forth, and set the corresponding frequency and number of pressurizations, etc.

[0037] Furthermore, the system also includes a self-adjusting module 23, which is connected to the programmable interface 22.

[0038] In this specific embodiment, during actual use, by setting the self-adjustment module 23, the brightness of the programmable interface 22 can be automatically adjusted according to the ambient light level.

[0039] In the specific use of the biomechanical system for cell and tissue culture and biomaterial preparation according to the present invention, cells, tissue-engineered tissues, and biomaterial raw materials are placed into the tube 17, and the front end of the tube 17 is sealed with the screw cap 18, making the carrier a bioreactor that can withstand pressure of not less than 4 MPa. Then, the carrier is placed inside the metal sleeve 14, and the drive assembly is activated. The drive assembly drives the drive wheel 10 to rotate, and the drive wheel 10 drives the driven wheel 11 to rotate via the belt 12. The driven wheel 11 drives the transmission component 13 to rotate. This movement causes the screw 7 to move, which in turn moves the pressure sensor 8. The pressure sensor 8 then moves the metal shaft 15, causing the piston 16 to slide within the tube 17 to apply pressure. Simultaneously, the monitoring sensor 3 monitors the internal hydrostatic pressure (or pressure intensity), oxygen partial pressure, carbon dioxide partial pressure, pH value, glucose, and lactic acid within the tube 17. This monitoring is achieved through the circulation module 5. This approach solves the technical problems of existing biomechanical devices, such as excessive size, weight, and operating costs due to integrated equipment, poor mechanical control accuracy, and limited pressure application.

[0040] By adopting this invention, manufacturing and usage costs are significantly reduced, while achieving small size, light weight, and easy transportation. In addition, it can provide a high pressure value, which can simulate the mechanical conditions experienced by any organ or tissue in the human body, and greatly improve the accuracy of mechanical control.

[0041] The above description discloses only one preferred embodiment of the present invention, and should not be construed as limiting the scope of the present invention. Those skilled in the art will understand that all or part of the processes of the above embodiments can be implemented, and equivalent changes made in accordance with the claims of the present invention are still within the scope of the invention.

Claims

1. A biomechanical system for cell and tissue culture and biomaterial preparation, characterized in that, It includes a reactor module, a power pressurization module, monitoring sensors, a computer, and a circulation module. The reactor module is mounted on the power pressurization module. The monitoring sensors are connected to the power pressurization module. The computer is connected to the monitoring sensors. The circulation module is connected to both the sensors and the reactor module. The power pressurization module includes a frame, a drive assembly, a screw, and a pressure sensor. The screw and the drive assembly are mounted on the frame, and the drive assembly drives the pressure sensor to move via the screw.

2. The biomechanical system for cell and tissue culture and biomaterial preparation as described in claim 1, characterized in that, The drive assembly includes a servo motor, a drive wheel, a driven wheel, a belt, and a transmission component. The transmission component is rotatably connected to the frame. The screw is threadedly engaged with the transmission component. The drive wheel and the driven wheel are fixedly connected to the output end of the servo motor and the transmission component, respectively. The belt is disposed between the drive wheel and the driven wheel.

3. The biomechanical system for cell and tissue culture and biomaterial preparation as described in claim 2, characterized in that, The reactor module includes a metal sleeve, a support member, a metal shaft, and a piston. The metal sleeve is mounted on the frame, the support member is placed inside the metal sleeve, the piston is slidably connected to the support member, and the piston is fixedly connected to the metal shaft.

4. The biomechanical system for cell and tissue culture and biomaterial preparation as described in claim 3, characterized in that, The metal sleeve has an opening for connecting the carrier to the monitoring sensor and the circulation module.

5. The biomechanical system for cell and tissue culture and biomaterial preparation as described in claim 2, characterized in that, The carrier includes a tube body and a spiral cap. The tube body is placed inside the metal sleeve, and the spiral cap is threadedly connected to the tube body.

6. The biomechanical system for cell and tissue culture and biomaterial preparation as described in claim 5, characterized in that, The adjustable accuracy of the servo motor is 0.01 mm, and the accuracy of the pressure sensor is 0.01 kg, or 0.1 N.

Images