34 results about "Continuous perfusion" patented technology

The invention belongs to the technical field of organ chips, provides an in-vitro brain lymphatic system chip construction method and a brain lymphatic system chip, and makes up the blank of brain lymphatic system in-vitro model construction. The method comprises the following steps: firstly, carrying out flushing treatment, disinfection and sterilization treatment, rinsing treatment and cell culture on a micro-fluidic channel in the chip; then, in the treated micro-fluidic channel, blood vessel channel cell inoculation and brain parenchyma channel cell inoculation are carried out; and finally, in the blood vessel channel, perfusion is carried out once at a preset flow speed every preset duration, shear force stimulation simulating pulse type blood flow is provided, basic formation of in-vitro cerebral vessels is achieved after continuous perfusion, perfusion is carried out once at a preset flow speed every preset duration, shear force stimulation simulating pulse type blood flow is provided, and in-vitro cerebral vessels are formed. Intermittent liquid flow simulating arterial fluctuation is achieved.

The utility model relates to a continuous perfusate sheath support for ear endoscope surgery. The device comprises an elastic support body which is integrally columnar, the support body comprises a tubular inner wall and a tubular outer wall, the upper end of the inner wall is connected with the upper end of the outer wall through an annular top wall, the lower end of the inner wall is connected with the lower end of the outer wall through an annular bottom wall, and the inner side of the inner wall is an instrument channel of the support body; a cavity is formed between the inner wall and the outer wall, an infusion tube opening communicated with the cavity is formed in the annular top wall or the edge of the upper end of the outer wall, an infusion tube or an infusion tube connector is arranged on the infusion tube opening, and a plurality of liquid outlet holes which are evenly distributed and communicated with the cavity are formed in the annular bottom wall.

The invention relates to a high-capacity continuous filling system, which relates to the technical field of liquid filling and comprises a filling unit for filling milk, a forming unit for shaping a filled packaging material and two rack plates, wherein the forming unit comprises two sets of driving mechanisms, the two sets of driving mechanisms are symmetrically distributed along the rack plate, and the forming unit further comprises two shaping mechanisms and two positioning rods which are arranged on the driving mechanisms and used for shaping packaging materials. The high-capacity continuous filling system ensures that the capacity of each bag of products is in a strict control range, comprehensively matches the filling flow, the operating speed of the clamping jaw and the pressure and speed of extrusion forming, can effectively reduce fluctuation caused by too high or too low pressure in the forming process, further ensures the stability of the filling capacity, and improves the production efficiency. And the whole filling process is monitored and adjusted in real time by utilizing the PLC, including all links from liquid level detection to flow control to final packaging and forming, so that highly automatic operation is realized.

The invention relates to a medical continuous irrigation system (10) with an inner shaft (20) having a wall (21), a distal end (20a), a proximal end (20b), an inner shaft outer diameter (ISA) and an inner shaft length (ISL), and an outer shaft (40) having a wall (41), a distal end (40a), a proximal end (40b), an outer shaft inner diameter (ASI) and an outer shaft length (ASL), wherein a first connecting piece (30) is arranged at the proximal end (20b) of the inner shaft (20), which has a first axial through-opening (31) and on which a first connection (32) for an irrigation hose is arranged, wherein a second connecting piece (50) is arranged at the proximal end (40b) of the outer shaft (40), which has a second axial through-opening (51) for the inner shaft (20) and on which a second Connection (52) for a suction hose is arranged,and wherein at least one suction opening (46) is arranged in a section (45) of the wall (41) of the outer shaft (40) adjacent to the distal end (40a), wherein the distal end (20a) of the inner shaft (20) comprises an end face (23) on which a microchip (60) with at least one image-capturing area (61) and at least one light-emitting area (62) is arranged.

The utility model discloses a fixing device for otology continuous perfusion flushing recovery, which comprises a placing bin, a reflux treatment bin, a pump body and a flusher, a fixing auxiliary mechanism is arranged on the inner wall of the inner side of the placing bin, and the fixing auxiliary mechanism comprises a control frame fixedly connected to the inner wall of the inner side of the placing bin; a control rod is slidably connected to the interior of the control frame, and a clamping frame is fixedly connected to one end of the control rod. According to the fixing device for continuous perfusion, flushing and recycling in the otology department, the rotating shaft drives the regulation and control wheel to rotate by rotating the rotating shaft, the regulation and control wheel pushes the control rod to move by utilizing the shape of the regulation and control wheel, and the clamping frame is clamped on the outer side of the backflow treatment bin; the purpose that the clamping frame can conveniently limit the backflow treatment bin through the limiting frame is achieved, then the backflow treatment bin can be conveniently installed at the bottom of the inner side of the containing bin, and the situation that installation and fixation of the backflow treatment bin fail is avoided.

The invention relates to a cell tissue culture device special for primary cells, and relates to the technical field of cell culture, the cell tissue culture device comprises an incubator, the interior of the incubator is divided into three independent culture chambers through partition plates, each culture chamber is internally provided with at least three culture dishes, and the cell tissue culture device further comprises a dynamic culture assembly, at least two culture rooms are provided with a dynamic culture assembly, the dynamic culture assembly is provided with a micro-channel structure capable of simulating blood vessels, the dynamic culture assembly, the electrophysiological stimulation piece and the traction force simulation piece are integrated to form the culture system capable of simulating various physiological conditions in the body, the dynamic culture assembly simulates a blood vessel microchannel to realize low-shear-force continuous perfusion; the electrophysiological stimulation piece simulates a neuroelectric signal; the traction force simulation part applies controllable mechanical traction force through a multi-caliber breather pipe, and the three parts have a synergistic effect, so that the physiological function expression and culture quality of the primary cells are improved.

The present invention provides methods for producing, from pluripotent stem cells, natural killer (NK) cells or precursor cells thereof. The methods are: a method which is characterized by comprising a) a step for inducing hematopoietic progenitor cells (HPCs) from pluripotent stem cells in vitro, b) a step for amplifying the HPCs in vitro, and c) a step for inducing, from the HPCs, NK cells or precursor cells thereof in vitro, and which is characterized in that a histone methyltransferase and / or an allyl hydrocarbon receptor (AHR) is inhibited in one or more of steps a)-c); and a method which comprises (1) a step for forming, in a first culture medium, pluripotent stem cell spheres having an average particle size of 200 µm or more, (2) a step for inducing a cell population including HPCs through three dimensional culturing of the pluripotent stem cell spheres obtained in step (1) by using a second culture medium containing a histone methyltransferase inhibitor and / or an AHR antagonist, and (3) a step for inducing a cell population including NK cells or precursor cells thereof through three dimensional culturing of the cell population including the HPCs obtained in step (2) by using a third culture medium containing the histone methyltransferase inhibitor and / or the AHR antagonist, and in which one or more of steps (1)-(3) are performed through perfusion culturing, and preferably steps (1)-(3) are performed through continuous perfusion culturing.

The invention provides a drug loading device capable of achieving continuous perfusion, the drug loading device comprises a balloon dilatation catheter, a drug covered stent and a guide recovery catheter which are connected in sequence, the drug covered stent comprises a self-expanding base stent, a balloon assembly is arranged in the base stent, a covering film is arranged on the surface of the base stent, and the balloon assembly is connected with the guide recovery catheter. A medicine coating is arranged on the surface of the covering film, the base body support comprises a main body section capable of expanding in the radial direction, the two ends of the main body section are connected with fixing sections through connecting sections, and the balloon assembly is arranged in the main body section and fixed through the fixing sections. According to the embodiment of the invention, the medicine placement time can be prolonged, so that the medicine is better absorbed; meanwhile, the medicine loss of the medicine in the release process can be reduced, the harm of the medicine to other visceral organs due to the blood flow flushing effect is reduced, and the transfer rate of the medicine to blood vessels is increased; and as the placement time can be prolonged, the device can be used as a treatment device for vascular perforation.

The utility model discloses a kind of automatic continuous filling's corpse antiseptic high-pressure perfusion device, including liquid storage tank, medicament box, automatic liquid preparation liquid filling device, medicament box is sequentially connected liquid storage tank by medicament branch pipe, first solenoid valve, first flowmeter, medicament main pipe, the upper portion of liquid storage tank is connected distilled water tank by water pipe, second flowmeter, second solenoid valve;Automatic liquid preparation liquid filling device includes liquid pump, controller and liquid level sensor, the import of liquid pump is connected liquid storage tank by liquid extraction pipe, the outlet of liquid pump is connected perfusion needle by gate valve, liquid guide pipe, hose;Liquid level sensor is arranged in liquid storage tank, and upper and lower limit switch of liquid level sensor is electrically connected with first solenoid valve, second solenoid valve, liquid pump switch and controller respectively.The utility model integrates automatic liquid preparation and high-pressure liquid filling function, can continuously, stably obtain sufficient perfusion fluid supply, realizes high pressure, automatic, continuous perfusion, improves perfusion efficiency and uniformity.

This invention belongs to the field of organ-on-a-chip technology, proposing a method for constructing an in vitro brain lymphatic system chip and a brain lymphatic system chip, filling the gap in the construction of in vitro models of brain lymphatic systems. First, the microfluidic channels in the chip are rinsed, sterilized, washed, and cultured with cells. Then, vascular channel cells and brain parenchyma channel cells are seeded in the treated microfluidic channels. Finally, in the vascular channels, perfusion is performed at a preset flow rate and at preset intervals to provide shear force stimulation mimicking pulsed blood flow. Continuous perfusion achieves the basic formation of in vitro brain blood vessels. The intermittent fluid flow mimicking arterial pulsations is achieved through this preset flow rate and preset interval perfusion.

The present invention discloses a sustainable underwater operation ear endoscope, comprising: a housing, an imaging device for inserting into the ear and photographing, a lighting system for providing lighting to the imaging device photographing area, and a cleaning system for inserting into the ear to clean the photographing area. The cleaning system, the imaging device, and the lighting system are all arranged on the housing, and the cleaning system is arranged on the imaging device so that the cleaning system and the imaging device are simultaneously inserted into the ear, and the lighting system is connected to the imaging device. The imaging device can photograph the situation in the ear and generate an image. Since there is no light in the ear, the lighting system is required to provide lighting. The cleaning system and the imaging device are inserted into the ear at the same time. When the area photographed by the imaging device bleeds or bone needs to be cleaned, the cleaning system can be connected to the cleaning system using an external water source to continuously perfuse and circulate the area photographed by the imaging device, so that the field of vision can always be kept clear, no other auxiliary devices are required, and no interruption of the surgical operation is required, thereby improving the surgical efficiency.

The present disclosure is directed to a method of sub-milliliter perfusion to mimic continuous perfusion in lab-scale bioreactors comprising: establishing a cell culture in a media in at least one well of a multi-well culture plate, wherein the working volume of media is less than 1 mL, and expanding the cell culture in the multi-well culture plate.

To provide a drip chamber for cerebrovascular catheter surgery that allows visual confirmation of a stop in drug-solution perfusion, thereby solving the problem in which a water-sealing method, in which a pressure bag is used to press the entire infusion bag for not allowing air to enter the drip chamber thereby filling it with a drug solution and performing perfusion, increases the risk of thrombosis because surgery may continue without recognizing that the perfusion of the drug solution has stopped or ended.SOLUTION: By fluid pressure from continuous perfusion of the drug solution passing through the drip chamber, a structure set inside the drip chamber moves, and when perfusion stops, movement of the structure stops, which is visually recognizable from outside.SELECTED DRAWING: Figure 1

The application relates to the technical field of medical devices, and discloses a perfusion device which comprises a shell, a rotor, a push plug and a fluid baffle, the shell comprises a shell body, a fixed shaft is arranged at the opening of the shell body, a fluid outlet and a fluid inlet are arranged on the sidewall of the shell body; the rotor comprises a rotor body and a handle, the rotor body is rotatably arranged in the opening through the fixed shaft, an annular cavity for containing fluid is formed between the outer circumferential wall of the rotor body and the inner wall of the shell body, the fluid outlet and the fluid inlet are both communicated with the annular cavity; the push plug is arranged on the outer circumferential wall of the rotor body, and the push plug is in sealing abutment with the inner wall of the shell body; the fluid baffle is sealingly connected in the annular cavity, one end of the fluid baffle is fixed to the inner wall of the shell body, the other end of the fluid baffle is in abutment with the outer circumferential wall of the rotor body, and the fluid baffle can be elastically deformed under the pushing of the push plug so that the push plug can pass through. The perfusion device provided by the application realizes continuous perfusion of fluid, and can timely and finely adjust the perfusion flow and the flow rate according to the intraoperative situation and pressure feedback.

The application provides a method for evaluating drug accumulation toxicity based on ex vivo rabbit corneal long-term perfusion, and belongs to the technical field of drug accumulation toxicity evaluation. The method comprises the following steps: preparing a perfusion sample and building a perfusion device; performing continuous perfusion, collecting corneal physiological response data and drug concentration monitoring data in real time; performing denoising processing on the collected original data, and extracting drug accumulation toxicity characteristic parameters; inputting the toxicity characteristic parameters into a drug accumulation toxicity evaluation model based on a convolutional neural network to obtain a corneal toxicity grade under the current perfusion condition; establishing a multi-objective analysis model, solving the model, and obtaining a quantitative evaluation result of drug accumulation toxicity and a safe perfusion threshold; and determining the corneal toxicity risk grade of the drug and the safe use concentration range. The application ensures the reliability of the data through a denoising algorithm and a feature extraction method, realizes accurate toxicity determination by using a neural network, improves the scientificity and efficiency of the evaluation, and provides a precise toxicity evaluation method for animal ophthalmic drug research and development.

The invention provides perfusion equipment and method for organ transfer, and relates to the technical field of medical instruments.The perfusion equipment comprises an organ bin, a perfusion tank, a driving pump, an oxygenator, a first-stage bubble discharging assembly, a bubble detection part, a second-stage bubble discharging assembly, a pipeline assembly and a valve device; the bubble detection part detects whether the perfusate contains bubbles or not in real time; the first-stage bubble discharging assembly and the second-stage bubble discharging assembly form two-stage gradient bubble removal; the pipeline assembly comprises a main pipeline and branch pipelines, and the main pipeline forms a main filling circulation path; the secondary bubble discharging assembly is connected to the main pipeline in parallel through the branch pipeline. The valve device switches the conveying pipeline to ensure that no bubble enters the organ bin. According to the invention, through two-stage gradient bubble removal, the ability to deal with sudden bubbles is improved; the storage tank continuously supplies liquid to guarantee continuous filling of the organ bin; the organ hypoxia injury caused by shut-down bubble removal is avoided; the purpose of improving the safety, continuity and stability of the filling equipment is achieved.

The invention relates to a continuous irrigation system (10) for medical use, comprising: an inner shaft (20), which has a wall (21), a distal end (20a), a proximal end (20b), an inner shaft external diameter (ISA) and an inner shaft length (ISL); and an outer shaft (40), which has a wall (41), a distal end (40a), a proximal end (40b), an outer shaft internal diameter (ASI) and an outer shaft length (ASL), wherein at the proximal end (20b) of the inner shaft (20) there is a first connecting piece (30), which has a first axial through-opening (31) into which there opens a first connector (32) for an irrigation hose, wherein at the proximal end (40b) of the outer shaft (40) there is a second connecting piece (50), which has a second axial through-opening (51) for the inner shaft (20) and on which a second connector (52) for a suction hose is arranged, wherein at least one suction opening (46) is arranged in a portion (45) of the wall (41) of the outer shaft (40) adjoining the distal end (40a), and wherein, when the inner shaft (20) has been inserted into the outer shaft (40), an annular space (70) is formed between the inner shaft (20) and the outer shaft (40) and a portion (25) of the distal end (20a) of the inner shaft protrudes beyond the distal end (40a) of the outer shaft (40), wherein the distal end (20a) of the inner shaft (20) has a circumferential thickening (24) with an external diameter (VA) that corresponds to the outer shaft internal diameter (ASI), wherein at least one channel (60) with a first end (61) and a second end (62) is arranged in the thickening (24), and wherein the first end (61) of the channel (60) is arranged in the portion (25) and the second end (62) opens into the annular space (70).

The invention belongs to the technical field of medical instruments, and provides a continuous flushing type balloon catheter device, an inner cavity is used for continuous perfusion of heparin water, an outer cavity is used for balloon dilatation to inject liquid or air, the device is stably fixed in a blood vessel through balloon dilatation, continuous perfusion of liquid in the blood vessel is achieved, and the continuous flushing type balloon catheter device is convenient to use. Therefore, the donor keeps the blood vessel (radial artery or superficial temporal artery) to keep certain tension to avoid distortion caused by collapse, continuous perfusion keeps the blood vessel cavity of the donor blood vessel clean and moist, the condition of vascular embolism blockage caused by foreign matters or thrombus in the donor blood vessel is avoided, thrombus formation is fundamentally prevented, and the donor blood vessel is protected from being damaged. Meanwhile, the continuous perfusion can enable the intracranial vascular anastomotic stoma to be in an open state, so that the smooth rate of vascular anastomosis can be kept, the operation area can be kept clean, and the situation that surrounding blood pollutes the anastomotic stoma and affects the smooth rate of the operation is avoided.

To provide a persistent perfusion sheath assembly for passing an intravascular medical device while maintaining a flow passage in the vessel.SOLUTION: The sheath assembly passes through an arteriotomy site and can include a first lumen 120 configured for the passage of a medical device, and a second lumen 130 configured to allow a flow of fluid from a location in the blood vessel upstream of the arteriotomy site to a location downstream of the arteriotomy site. The sheath assembly can further include a stylet, a sleeve or an additional sheath configured to selectively open and close the flow of blood between upstream and downstream of the arteriotomy site. The sheath assembly can further include a closure device distal of the arteriotomy site, the closure device configured to control the blood flow through the arteriotomy site.SELECTED DRAWING: Figure 1

Medical continuous irrigation system (10) with an inner shaft (20) having a wall (21), a distal end (20a), a proximal end (20b), an inner shaft outer diameter (ISA) and an inner shaft length (ISL), and an outer shaft (40) having a wall (41), a distal end (40a), a proximal end (40b), an outer shaft inner diameter (ASI) and an outer shaft length (ASL), wherein a first connection piece (30) is arranged at the proximal end (20b) of the inner shaft (20) having a first axial through-opening (31) into which a first connection (32) for an irrigation hose opens, wherein a second connection piece (50) is arranged at the proximal end (40b) of the outer shaft (40) having a second axial through-opening (51) for the inner shaft (20) and on which a second connection (52) is arranged for a suction hose,wherein at least one suction opening (46) is arranged in a section (45) of the wall (41) of the outer shaft (40) adjacent to the distal end (40a), and wherein, when the inner shaft (20) is inserted into the outer shaft (40), an annular space (70) is formed between the inner shaft (20) and the outer shaft (40), and the distal end (20a) of the inner shaft projects beyond the distal end (40a) of the outer shaft (40) with a section (25), wherein the inner shaft (20) has a circumferential thickening (24) at the distal end (20a) with an outer diameter (VA) corresponding to the inner diameter (ASI) of the outer shaft, wherein at least one channel (60) with a first end (61) and a second end (62) is arranged in the thickening (24), wherein the first end (61) of the channel (60) is arranged in the section (25), and the second end (62) opens into the ring space (70).

The utility model discloses a continuous perfusion device, which relates to the field of perfusion tools, and adopts the technical scheme that the continuous perfusion device comprises a cylinder body, a transition pipe and a liquid inlet pipe, a piston is arranged in the cylinder body, a push rod is fixed on the piston, and the rear end of the push rod extends backwards to the rear part of the cylinder body; the front end of the cylinder body is communicated and butted with the rear end of the transition pipe; a first one-way valve is arranged in the front section of the transition pipe and only allows liquid to flow forwards; the upper end of the liquid inlet pipe is communicated and butted with the rear section of the transition pipe; a second one-way valve is arranged in the liquid inlet pipe and only allows liquid to flow towards one end of the transition pipe. Continuous filling of liquid can be achieved, liquid can be supplemented and filled in each time of pumping, filling continuity can be remarkably improved, and efficiency is improved.

The invention provides a balloon perfusion catheter used in aortic dissection, and relates to the technical field of medical instruments.The balloon perfusion catheter comprises a perfusion catheter body and balloons evenly distributed on the periphery of the perfusion catheter body, a perfusion inlet is formed in the near end of the perfusion catheter body, and a perfusion outlet is formed in the far end of the perfusion catheter body; three balloons are distributed on the periphery of the perfusion catheter body, the near-end balloon is a blocking balloon, the middle balloon is a threaded balloon, and the far-end balloon is an expansion balloon. The balloon perfusion catheter can provide continuous perfusion in an aortic dissection operation, remarkably reduces or avoids long-time circulation stopping, effectively prevents perfusion blood from flowing back to an operation area from the inside and the outside of the trunk support through the synergistic effect of the three balloons, provides a clear operation view field for surgeons, and improves the operation efficiency. And accurate suture and reconstruction are facilitated, and the accuracy and safety of an operation are improved.

Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.

The present invention provides a perfusion culture chip and a perfusion system, comprising an upper sealing plate, a culture filter layer and a lower sealing plate layer, wherein the lower sealing plate is provided with an injection interface and a filtration interface, a filling channel corresponding to the position of the injection interface runs through the culture filter layer and is connected to the upper side wall of a culture chamber in the culture filter layer, and a filtration hole at the bottom of the culture chamber corresponds to the position of the filtration interface. The present invention adopts a method of filling the culture solution at the top and filtering it at the bottom for perfusion culture, and can realize continuous perfusion culture of microorganisms and cells.

The present disclosure provides an unsteady continuous perfusion cell culture method for producing proteins in a bioreactor. After entering the production stage, the cells are cultured in an unsteady state, so that the activity is reduced along with time. Productions of protein products using these cell culture methods are increased.

The utility model discloses a fixable sticking towel for fundus surgery, which comprises a hole towel sticking main body and a fixing strip, and the fixing strip is arranged on the left side of the hole towel sticking main body. The hole towel pasting body is mainly composed of pressure-sensitive paper and a thin film with an adhesive layer, and the thin film with the adhesive layer is pasted to the upper layer of the pressure-sensitive paper. The fixing strip is mainly composed of pressure-sensitive paper, a thin film with an adhesive layer and a thin film outer frame, the thin film outer frame is pasted on the upper layer of the pressure-sensitive paper, and the thin film with the adhesive layer is arranged on the upper layer of the thin film outer frame. According to the fixable sticking towel for the fundus surgery, through the design and use of the fixing strip and the thin film with the adhesive layer on the fixing strip, an infusion tube can be firmly fixed in the surgery process, continuous infusion of perfusate in eyes is guaranteed, and therefore the problems that in the surgery process, the infusion tube slips, and the surgery effect is affected are effectively solved.

The present application relates to the technical field of interventional medical instruments, in particular to a pressure-controllable super-high-oxygen myocardial perfusion device and a pressure control method. The device comprises a normal-pressure oxygenation cavity, a heat exchange cavity and a high-pressure oxygenation cavity connected in sequence, and a main circulation loop and a static high-pressure oxygenation bypass; the inlet and outlet of the high-pressure oxygenation cavity are both provided with a blockage type peristaltic pump with pumping and sealing functions, the peristaltic pump is internally provided with an oxygenation membrane, and is configured with a pressurizing mechanism for simultaneously pressurizing liquid sides and oxygen sides on both sides of the oxygenation membrane, and a heat exchange mechanism for cooling the perfusion liquid to about 14 DEG C. The present application can prepare super-high-oxygen myocardial protective liquid with oxygen partial pressure higher than 700 mmHg through static, low-temperature and high-pressure oxygenation while maintaining extracorporeal circulation continuous perfusion, significantly improve the myocardial protection effect, and has the advantages of compact structure, precise control and safe operation.

The present invention provides a method for producing natural killer (NK) cells or precursor cells thereof from pluripotent stem cells, comprising a) a step of in vitro inducing hematopoietic progenitor cells (HPC) from pluripotent stem cells, b) a step of in vitro expanding HPC, and c) a step of in vitro inducing NK cells or precursor cells thereof from HPC and inhibiting histone methyltransferase and / or aromatic hydrocarbon receptors (AHR) in one or more of steps a) to c); and a method comprising (1) a step for forming pluripotent stem cell spheres having an average particle diameter of 200 [mu] m or more in a first culture medium, (2) a step for inducing the pluripotent stem cell spheres obtained in step (1) into a cell population containing HPC by three-dimensional culture using a second culture medium containing a histone methyltransferase inhibitor and / or an AHR antagonist, and (3) a step for inducing the pluripotent stem cell spheres obtained in step (2) into a cell population containing HPC. And (3) a step in which the cell population containing HPC obtained in step (2) is induced into a cell population containing NK cells or precursor cells thereof by three-dimensional culture using a third culture medium containing a histone methyltransferase inhibitor and / or an AHR antagonist, and one or more of steps (1) to (3) are performed by a perfusion culture method. Steps (1) to (3) are preferably performed by a continuous perfusion culture method.

The utility model discloses a handheld continuous perfusion device and a flexible ureteroscope multifunctional sheath system. The handheld continuous perfusion device comprises a shell, a manual rocking handle, a reciprocating lead screw, a piston type injection device, a one-way valve, a device liquid inlet and a device liquid outlet. The piston type injection device comprises two water inlets and outlets, two pistons and two injection barrels, the two injection barrels are arranged on the same straight line, the two water inlets and outlets are oppositely arranged, one piston is arranged in each injection barrel, and the two pistons are connected with each other; the two water inlets and outlets are respectively connected with the device liquid inlet through a one-way valve and are respectively connected with the device liquid outlet through a one-way valve; and when the manual rocking handle rotates, the two pistons are driven by the reciprocating lead screw to reciprocate in the syringe respectively. Water can be fed and discharged continuously, the perfusion amount and the perfusion pressure can be dynamically adjusted in time in the operation, the perfusion pressure and the pelvis pressure are monitored in real time, and the operation safety is improved.

The invention discloses a method for carrying out in-situ sterilization and treatment on a vaccine microcarrier by using a bioreactor, and belongs to the field of cell culture. The method comprises the following steps: firstly, adding a microcarrier into a bioreactor, soaking and swelling the microcarrier in a PBS (Phosphate Buffer Solution), sterilizing the microcarrier, the bioreactor and each branch in situ by pure steam, and continuously perfusing and replacing the PBS by using a sterile cell culture solution. Steam is directly in full contact with the microcarrier, sterilization has no dead angle, and the effect is remarkable; the whole process is sterile and closed, pollution risks caused by human intervention are avoided, and efficient replacement is achieved through continuous perfusion. The method solves the problems of incomplete traditional sterilization, tedious operation and high pollution risk, is suitable for high-density culture of the adherent cells, and is high in practicability.