44 results about "Monoculture" patented technology

A system has been constructed that recapitulate the features of a capillary bed through normal human tissue. The system facilitates perfusion of three-dimensional (3D) cell monocultures and heterotypic cell co-cultures at the length scale of the capillary bed. A major feature is that the system can be utilized within a “multiwell plate” format amenable to high-throughput assays compatible with the type of robotics commonly used in pharmaceutical development. The system provides a means to conduct assays for toxicology and metabolism and as a model for human diseases such as hepatic diseases, including hepatitis, exposure-related pathologies, and cancer. Cancer applications include primary liver cancer as well as metastases. The system can also be used as a means of testing gene therapy approaches for treating disease and inborn genetic defects.

The invention provides an efficient three-dimensional planting method for sweet sticky corn, in order to solve the problem of low planting benefit of the existing sweet sticky corn monoculture. The efficient three-dimensional planting method comprises the following steps: after performing spring ploughing and soil preparation, defining 1-1.1m area as a region, and coating a mulching film on each region; in the last ten-day period of April, planting a row of breeding melons on the mulching film; in the last ten-day period of May, planting a row of sweet sticky corn between two mulching films; from the last ten-day period of September to the first ten-day period of October, after harvesting the breeding melons and the sweet sticky corn in turn, preparing soil, fertilizing and planting winter wheat; in the last ten-day period of April in the next year, planting two rows of sweet sticky corn in preserved blank rows; and harvesting the winter wheat at the end of June, and planting autumn vegetables on the space formed after the winter wheat is harvested. According to the efficient three-dimensional planting method provided by the invention, the output benefit of unit area is increased, the crop rotation and the sowing and soil improving operation are organically combined, the use ratio of limited cultivated land resource is efficiently improved and the promotional value is high.

Aquafeed, animal feed, and other food products, as well as nutritional and pharmaceutical compounds, chemicals and biomaterials are important commodities that can be produced at commercial scale by fermentation of microorganisms. The present invention provides a method for producing these valuable multi-carbon compounds from simple gas feedstocks, such as carbon dioxide, hydrogen and oxygen, by cultivating a consortium of microbial cells specially selected for this purpose in an aqueous culture medium. In addition to exploiting inexpensive feedstocks, such as waste industrial gas for this cultivation, the platform described herein also provides the advantage of removing carbon dioxide and other waste gases from industrial emissions, which would otherwise contribute to global climate change. Furthermore, the cultivation of a microbial consortium can provide highly nutritious components to a feed blend that might not be available from a monoculture.

The invention relates to the technical field of peanut planting and cultivation in saline and alkaline land, in particular to a cottonseed intercrop-planting method for achieving dual high peanut / cotton yields in saline and alkaline land. The method comprises the following steps that (a) land preparation and salt content restraining are performed; (b) timely ploughing and land preparation are performed; (c) fertilization is performed; (d) timely sowing is performed, wherein 4:2 equal-amplitude cottonseed intercropping mode is adopted for peanuts and cotton, and the floor area is kept to 1:1; (e) film covering is performed, wherein a convention planting and film covering planting mode is adopted. The yield level of cotton monoculture in a conventional mode is kept by adopting the equal-amplitude cottonseed intercropping mode, the peanut yield of 109-159 kg / mu is increased compared with monoculture peanuts (the yield is 170.5 kg / mu) in moderate-severe saline and alkaline land, the peanut yields of 50.76 kg / mu and 31.23 kg / mu are increased by adopting the 4:2 equal-amplitude cottonseed intercropping mode of peanuts / cotton compared with the yields obtained by adopting the proportions of 2:1 and 6:3, the anti-risk capability of an agricultural planting structure in the saline and alkaline land is improved, and the dual high peanut / cotton yields in the moderate or severe saline and alkaline land are achieved.

The invention discloses a pea and tobacco intercropping method. According to the method, based on the technical background of tobacco monoculture in the southwestern tobacco-growing area, pea seeds are sown on two sides of a tobacco ridge after laminas on the lower middle portion of tobacco are harvested or after harvest is completed, and a plastic net is hung on a tobacco stem as the climbing support of a pea, and then pea and tobacco intercropping is achieved. The pea and tobacco intercropping method has the advantages that the multiple crop index of land is improved; fertilization is not needed for pea planting, a large quantity of nitrogenous fertilizer and potash fertilizer left in tobacco land can be utilized, and the economical benefit of tobacco farmers is increased by 1000-1200 yuan / mu; the tobacco stem is used as the climbing carrier of the pea, and cost of 200-400 yuan / mu for building a support for the pea is saved; and due to the fact that the height of the tobacco stem can not meet climbing requirements of the pea, the plastic net is laid on the top of the tobacco stem by the applier to enable the pea to continue to climb after reaching to the top of the tobacco stem, and technical prejudice of technicians in the field is overcome.

The present disclosure provides co-cultures of human pluripotent stem cell derived hepatocytes and at least one non-parenchymal cell population in vitro, methods of preparing the co-cultures and methods of using the co-cultures for high throughput screening and evaluation of drug candidates. The stem cell derived hepatocyte co-culture system provides an in vitro model in which cell viability and relatively mature hepatocyte phenotype of stem cell derived hepatocytes are maintained for extended periods relative to conventional monoculture.

It used as carrier of bioactive substances of natural origin like dry herb extracts or combinations thereof, other vegetable products as essential oils, fruit concentrates, animal products as bee products, added to vitamins combinations, said product ensuring bacteria proliferation and growth when stored and after intake. The bioactive products can be stored for period 2 to 3 years without the probiotic losing its activity. In the gastroinitestinal tract they can colonize supplying the body with useful substances resulting from their metabolism ensuring fast transportation of bioactive substances to body's cells. It contains low-lactose sour-milk product, low-lactose hydrolyzate with more than 95% degraded milk casein, saccharose, pectin, full-cream or skimmed low-lactose cow's milk fermented using selected strains of Lactobacillus delbrueckii subsp. bulgaricus, and additionally lyophilized monoculture of Lactobacillus delbrueckii subsp. bulgaricus or lyophilized mixture of monocultures of Lactobacillus bulgaricus, Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus lactis and dextrose.

The invention relates to the technical field of ecological food production technology and low carbon agriculture, and discloses a method for producing ecological formula rice in fields. The method comprises the following steps of: soaking seeds and raising rice seedlings (mixed sowing), rice field fertilization and seedling transplanting (companion planting), fencing to breed ducks and integrated rice-duck farming, and harvesting, wherein the key technologies of selecting and proportioning two soaking seeds of the rice, the companion planting of the rice, and integrated rice-duck farming management and water management and the like are optimized; a method for directly producing the ecological formula rice in the fields is obtained; the conventional means and relevant production restriction of producing the formula rice in workshops are changed; the later factory settings and work load of producing the formula rice are effectively reduced; the investment and energy use are reduced; the milled rice ratio, the amylose content and the gel consistency of the produced ecological formula rice are improved relative to the rice produced by the conventional monoculture method; and the ecological formula rice is free of pesticide, healthy and safe, and has obvious economical effect and social effect.

The invention provides a parent culture method in the industrialized cultivation of giant salamanders, which adopts adaptive culture facilities and culture methods according to the physiological characteristics of the giant salamanders and the gonad development law of the parent; and the diversification and quantification of the fed bait meet the nutritional requirement of the giant salamanders in pre-reproductive period. Simultaneously, the invention adopts the method of combining group-culture with monoculture according to the characteristics of the reproductive performance of an individual giant salamander, conducts electronic marking on group-culture parents, establishes individual archive for each parent, adopts track management and well does jobs such as daily water exchange, feeding, antepartum culture and postpartum culture, overwintering management and prevention of diseases. The parent culture method greatly saves culture area, lowers culture cost, improves the culture capacity of a unit area by 4 to 6 times, and the artificial propagation performance of giant salamanders by 30 to 40 percent and increases culture benefit.

The invention discloses a cultivation method for intercropping of corn and potatoes, and relates to a cultivation method for intercropping of corn and potatoes. The problem that an existing method forintercropping of corn and potatoes cannot sufficiently utilize optical energy and is low in soil resource utilization rate is solved. The method comprises the steps of 1 soil preparation, 2 seed selection and processing, 3 field arrangement, 4 proper sowing and rational close planting, 5 scientific fertilization, 6 precision management and 7 proper harvesting. Compared with monoculture of corn, the soil organic content is increased by 8.5%, and the soil total nitrogen content is increased by 6.4%; the bacterial community number in surface soil microflora is increased by 15.4%, and the mycorrhiza fungi community is increased by 32.6%. The potato yield is 8,700 kg / hm<2>, the corn yield is 11,395 kg / hm<2>, and the corn and potato total income is increased by 4,590 yuan / hm<2>. The method is applicable to the field of corn and potato planting.

The invention discloses a cultivation method for intercropping of corn and pepper, and relates to a cultivation method for corn and pepper. The problems that the lower middle portion of an existing monoculture corn population is insufficient in illumination and low in solar energy utilization rate, the top of a monoculture pepper population is excessively illuminated, and sunscald is likely to happen are solved. The method comprises the steps of 1 land selection and soil preparation, 2 seed selection and treatment, 3 intercropping mode, 4 pepper seedling raising and seedling management, 5 proper corn sowing and reasonable close planting, 6 proper pepper transplanting, 7 scientific fertilization, 8 precision management and 9 proper harvesting. The relative illumination intensity of the lower middle portion of the corn population is improved by 10.7-43.1% compared with monoculture corn; illumination of the top layer of the pepper population is only 50-60% that of a monocropping pepper population, the yield of fresh pepper is 5,344 kg / hm<2>, the corn yield is 11,642 kg / hm<2>, and the yield is increased by 11.5% compared with monocropping of corn. The method is used for the field of corn and pepper planting.

The invention discloses an efficient interplanting method of local special vegetables. Seeding furrows are dug in a vineyard in early April. Subterraneous stems of myoga ginger facing up are placed in the seeding furrows and are earthed according to the row spacing of 30-40cm. When buds grow to 11-13cm long, water is added to dilute decomposed people and livestock urine by 500-1000 times (by weight), and irrigation are performed plant by plant. When leaf sheaths of myoga ginger spread fully in May, 10kg of urea is applied in holes per mu. Before myoga ginger ears up in June, 15kg of potassium chloride is top-dressed per mu and the roots are covered with plant ash. The oil is kept moist during the whole growth period of myoga ginger. Grape is cultivated by the same monoculture method. The efficient interplanting method of local special vegetables is simple and high in planting efficiency. The yield of myoga ginger reaches 150kg / mu by interplanting myoga ginger in the vineyard.

The invention provides a green planting method for controlling diseases and insect pests in a rice field. While planting rice, upland rice is interplanted, and different types of rice are intercropped by utilizing the characteristics of large genetic background differences of rice varieties, which has a very obvious control effect on rice blast , the control effect is as high as 68.53%, which is a major innovation in the continuous control of rice blast using biodiversity at home and abroad. Compared with the net planting, the yield of the intercropping model of hybrid rice and bar rice increased by 19.71%. The present invention further adopts the planting mode of planting nectar source plants such as Thioflower and Zinnia on the ridge of rice fields (especially the mixed planting of Thioflower and Zinnia in proportion), increases biodiversity, and adjusts the insect population in the farmland ecology structure, increase the number of natural enemies of pests (such as beneficial insects such as parasitic wasps), and reduce crop pests.

The invention discloses a method using lavender to control flue-cured tobacco plant diseases and insect pests. The method includes the steps of changing a monoculture method of flue-cured tobaccos, firstly transplanting the lavender 30 days before transplanting of the flue-cured tobaccos, and planting one row of lavender and one row of flue-cured tobaccos. Through adoption of growth habit difference between the lavender and the flue-cured tobaccos, the lavender has specific aromatic odor, and volatile constituents of the lavender have a bactericidal effect and can eliminate the plant diseases and insect pests. In addition, the lavender is less in the plant diseases and insect pests, so the lavender can enable the incidence of the plant diseases and insect pests of the flue-cured tobaccos to be reduced. Through intercropping between the lavender and the flue-cured tobaccos, the method can be used for controlling occurrence of various plant diseases and insect pests of the flue-cured tobaccos, reducing usage amount of pesticide, and achieving the purposes of maintaining ecological balance and reducing insect control cost.

The invention discloses a method using sweet basil to control flue-cured tobacco plant diseases and insect pests. The method includes the steps of changing a monoculture method of flue-cured tobaccos, firstly transplanting the sweet basil 20 days before transplanting of the flue-cured tobaccos, and planting one row of sweet basil and one row of flue-cured tobaccos. Through adoption of growth habit difference between the sweet basil and the flue-cured tobaccos, the sweet basil has specific aromatic odor, and volatile constituents of the rosemary have a bactericidal effect and can eliminate the plant diseases and insect pests. In addition, the sweet basil is less in the plant diseases and insect pests, so the sweet basil can enable the incidence of the plant diseases and insect pests of the flue-cured tobaccos to be reduced. Through intercropping between the sweet basil and the flue-cured tobaccos, the method can be used for controlling occurrence of various plant diseases and insect pests of the flue-cured tobaccos, reducing usage amount of pesticide, and achieving the purposes of maintaining ecological balance and reducing insect control cost.

The invention discloses a flue-cured tobacco planting method using lavender to improve quality of tobacco leaves. The method includes the steps of making use of interplanting between the lavender and flue-cured tobaccos, changing a single mode of flue-cured tobacco planting, and forming a '1+1' planting mode of planting one row of flue-cured tobaccos and one row of lavender. The application effects indicate that the flue-cured tobaccos which grow in an interplanting mode are obviously superior to the flue-cured tobaccos which grow in a single planting mode in data of plant height, stem diameter, leaf area, effective leaf numbers, single leaf weight and single plant yield. The detection results indicate that beta-ocimene is detected in a tobacco sampling in an interplanting mode, and the beta-ocimene is not detected in the tobacco through monoculture, so the tobacco leaves can absorb aroma components of the lavender to some extent in the interplanting mode. Through sensory evaluation, the sensory quality of the flue-cured tobaccos in the interplanting mode is superior to the flue-cured tobaccos through the monoculture. Therefore, the flue-cured tobacco planting method can effectively promote quality of the flue-cured tobaccos, and has high ornamental value due to the adoption of interplanting between aromatic crops and the flue-cured tobaccos.

The invention relates to a high-yield planting method for winter corn and belongs to the technical field of planting, and particularly relates to a culture technology of winter corn. The invention provides the high-yield planting method for winter corn which is high in germination rate, strong in frost-resistance and strong in water and fertilizer absorption. The high-yield planting method for winter corn is characterized by comprising the following steps of: I, a seeding stage: a frostless region must be selected for planting winter corn; II, seeding specification and density: for high-yield culture of winter corn, the seeding specification is specified cultivation with wide and narrow row spacing, and a wide and narrow row spacing compound region is determined due to monoculture or intercropping; III, selection of a parcel of field suitable for planting winter corn: a parcel of field which has irrigation and drainage conditions and is loose in soil are selected to plant winter corn, the soil is maintained moist frequently, and robust growth of plants is promoted; and VI, film coverage: as the air temperature is relatively low for winter corn, the field capacity of the soil is reduced as a result of ending of rainy seasons, less rainfall and much wind.

The invention discloses a method for controlling rice blast and sheath blight by intercropping sagittaria sagittifolia in a rice field, and particularly relates to a method for planting rice and sagittaria sagittifolia in an intercropping way and harvesting mature rice and mature sagittaria sagittifolia in sequence. Field experiment results prove that with intercropping, rice and sagittaria sagittifolia have the symbiotic period in the field, so that the rice sheath blight infection rate of the rice intercropping cultivation is lower than that of rice monoculture; in an elongation stage, a heading stage, a filling state and a milk-ripe stage, the disease occurrence rates of the rice intercropping cultivation are respectively lower than that of the rice monoculture by 64.29%, 88.24%, 25% and 60%; furthermore, the rice blast infected leaf rate of the rice intercropping cultivation is lower than that of the rice monoculture on the whole, and the average control effect reaches up to 31.33%. Compared with the prior art, the method does not use chemical pesticides when being used for controlling the rice sheath blight and the rice blast, thus having obvious ecological economic benefits and guaranteeing the safe production of agricultural products; furthermore, field operation is convenient, and thus the method is convenient for actual popularization and application.

The invention discloses a calcium adding efficient ecological planting method in a corn and peanut intercropping mode. According to the method, 'flower cultivating 25' is taken as an experimental material, in corn||peanut 3:4 intercropping and peanut monoculture planting modes, different amounts of added calcium are set for treatment, and the yield of peanuts, the net photosynthetic rate of leavesand differences of the physiological property of the peanuts are studied; the study result shows that by adding calcium suitably, the yield of the peanuts in intercropping and monoculture modes can be further increased conveniently, the yield increasing effect on middle rows in the intercropping mode under the calcium adding premise is significantly higher than that of the monoculture mode, and acalcium fertilizer is applied additionally to increase the net photosynthetic rate of the peanuts, improve the activity of CAT, POD and SOD of peanut leaves in the monoculture and intercropping modes, and reduce the content of MDA of the peanut leaves. It is shown that calcium applying is beneficial for increasing the yield of the peanuts, improving the adaptability of the peanuts to shading, andimproving the photosynthetic production capacity of the peanuts.

The invention discloses a method for screening a corn genotype capable of improving nitrogen efficiency of a corn / soybean intercropping system, and belongs to the technical field of agriculture planting. The method is characterized by comprising the steps that genotype corn species with deep root, high ability of root secretion, and high system yield capable of being intercropped with soybean arescreened out, the corn species are divided to be deep root and shallow root and intercropped with soybean, the physiological mechanism of nitrogen efficiency difference of corn / soybean intercropping system is analyzed, and accordingly the corn genotype capable of improving the nitrogen efficiency of the corn / soybean intercropping system is screened out. The underground interaction mechanism of a monoculture system and the influence on the nitrogen fixation efficiency of intercropped soybean are researched by screening the corn species with different root system architectures and root secretions, thus the root system morphology physical features of the corn species suitable for the corn / soybean intercropping system are clarified, and the integral nitrogen efficiency of the intercropping system can be improved.

The invention discloses a double cropping rice yield increase method and relates to the technical field of agricultural planting. The method includes the following steps of during planting of double cropping rice, reducing application of chemical nitrogen fertilizer and adopting green manure for mixed cropping to achieve yield increase of double cropping rice. During adoption of the green manure for mixed cropping, four patterns are applied, wherein astragalus sinicus L. is adopted for monoculture AS under the first pattern, astragalus sinicus L. and ro-phanus sativus L.var.raphahistroides areadopted for mixed cropping AR under the second pattern, astragalus sinicus L. and lolium perenne L. are adopted for mixed cropping AL under the third pattern, and astragalus sinicus L., ro-phanus sativus L.var.raphahistroides and lolium perenne L. are adopted for mixed cropping ARL under the fourth pattern. Through research, it is determined that by adopting the green manure for replacing partialbasal application of chemical nitrogen fertilizer, increase of the yield of the double cropping rice is facilitated, and a great effect can be achieved by adopting astragalus sinicus L. for monoculture and adopting astragalus sinicus L. and ro-phanus sativus L.var.raphahistroides for mixed cropping.

The invention discloses a method for repairing medium-and-low-concentration mercury polluted farmland soil by using ramie monoculture. The method comprises the following steps: selecting farmland soilwith total mercury content of 0.3-3 mg.kg<-1>, scattering 0.030-0.300 kg.m<-2> of fulvic acid into the farmland soil, and then plowing the farmland soil; planting ramie cuttings into the farmland soil, and irrigating roots of ramie plants with a mixed aqueous solution including indolebutyric acid and naphthaleneacetic acid within 1-4 weeks after the plantation; and harvesting the overground partsof the ramie plants 5-6 months later, and repeating above steps to cultivate the ramie for multiple seasons until the total mercury content in the mercury polluted farmland soil is less than 0.3 mg.kg<-1>. The method of the invention significantly improves the absorption of mercury in the soil by the ramie, and effectively promotes the transfer of mercury in the roots of the ramie to the overground parts, so the repairing efficiency of the mercury polluted farmland soil is greatly increased.

The invention discloses an efficient planting method for intercropping of annual triple-cropping wheat, corn and beans. The planting method includes the steps of land preparation, wherein the land subjected to rotary tillage is prepared into a ridged field the width of which is 160 cm; sowing, wherein a combined sowing machine is adopted for completing sowing of the wheat, beans and corn accordingto a line ratio of 6:1:2 in the ridged field, the bandwidth of the wheat and corn is 80 cm, the line spacing of the wheat is 12 cm, the plant spacing of the wheat is 25-45 cm, the line spacing of thecorn is 40 cm, and the plant spacing of the corn is 25-45 cm; staggeredly planting the beans and the corn in lines between the wheat and the corn, wherein the spacing among the beans, the wheat and the corn is 15 cm, and the plant space of the beans is 25-45 cm. According to the provided efficient planting method for intercropping of the annual triple-cropping wheat, corn and beans, the yield isincreased by 10-40% under comparison with monoculture. By means of the method, the land utilization efficiency is improved, biological fertilizer culture of farmland is also achieved, and the method is an intensive, sustainable and efficient planting method.

The invention discloses a method for reducing the early bolting of Chinese angelica. The method includes selecting black soil and heilu soil with excellent conditions to perform sowing, the period ofcrop rotation being more than 3 years, performing shallow ploughing for one time before sowing, applying well-rotted farmyard manure, urea, diammonium phosphate and potassium sulfate before the soil is turned up, and killing pests; then transplanting seedlings, and selecting soybean seeds to perform dibbling; performing sterilization on the seedlings before transplanting, and performing air-dryingwhile performing soaking; performing transplanting in mid-to-late March, performing covering with wide films to perform dibbling, and planting one row of soybeans and three rows of Chinese angelica after mulching; during the transplanting of the Chinese angelica, respectively planting one seedling on the two sides of each hole, the distances of the terminal bud on the heads of the seedlings of the Chinese angelica from the mulching films being 2-3 cm, performing compacting with fine soil, and tightly sealing broken gaps; and during the dibbling of the soybeans, putting 2 soybean seeds in eachhole, performing compacting with the fine soil, and tightly sealing the broken gaps. The bolting rates after the intercropping of the Chinese angelica and the soybeans are lower than that of the monoculture of the Chinese angelica; the influence of different intercropping ratios on the bolting rates is different; when the Chinese angelica is intercropped with the soybeans in a ratio of 3:1, the bolting rate of the Chinese angelica is the lowest, which is only 26.06%; and the annual growth rate of the Chinese angelica can reach up to 66%.

The invention discloses a rotation mode capable of increasing yield of farmland in a cold alpine zone. The rotation mode capable of increasing the yield of the farmland in the cold alpine zone comprises the following steps: selecting a corn variety with strong drought tolerance and early maturity in early growth stage; and selecting a radix dipsaci asperoidis variety with strong cold resistance inlater growth stage. More specifically, in view of environmental characteristics, namely drought and rain in winter and spring as well as frequent early frost in autumn, of the cold alpine zone, the corn variety with strong drought tolerance and early maturity is selected for the early growth stage (the corn is sowed in 90-cm wide rows and 45-cm narrow rows with plant spacings of 40-50 cm after mulching in a spaced sowing way, each hole 3-4 grains); and the radix dipsaci asperoidis variety with strong cold resistance is selected for the later growth stage (the radix dipsaci asperoidis is sowedin interval rows between the corn in a hole-sowing way with row spacings of 45 cm and plant spacings of 25-30 cm, each hole 7-8 grains ). The rotation mode capable of increasing the yield of the farmland in the cold alpine zone adopts a grain and drug planting mode with highly efficient ecological cycle, and has reasonable layout. By changing a conventional corn monoculture mode into the corn-radix dipsaci asperoidis rotation mode, utilization efficiency of water, light and heat resources are greatly improved while ensuring low labor intensity and high operability, so that the rotation mode capable of increasing the yield of the farmland in the cold alpine zone is suitable for large-area promotion in vast alpine areas, especially in remote and backward alpine areas.