The invention relates to an ultrasonic couplant and a preparation method thereof. The raw materials for making the effective ingredient of the ultrasonic couplant comprise Radix polygoni multiflori, Angelica, Embelia laeta, Cirsium japonicum, Lonicera japonica stem, Donax cannaeform, Mentha haplocalyx, Eucalyptus tereticornis, Nidus Vespae, Nepal gongronema, Lysimachia candida, Herba Verbenae Officinalis, Euphorbia helioscopia, Astragalus sinicus, Solanum melongena and Daphne bark. The ultrasonic couplant provided by the invention has reasonable formulation and better coupling effect, integrates antibacterial and bactericidal, blood stasis removing and blood circulation promoting effects of traditional Chinese medicinal materials, has the advantages of no harm to a human body, no damage to a probe, no skin irritation and sensitization, no contamination of clothes, easiness in cleaning, lower cost, good stability, and so on, and avoids cross contamination in clinical ultrasonic examination. The ultrasonic examination with the ultrasonic couplant provided by the invention has better acoustic permeability and clearer ultrasonic imaging.