The invention relates to a weeding composition containing florasulam and clodinafop-propargyl, a preparation method and use thereof, wherein, the weeding composition is composed of a compound (A)-the florasulam and a compound (B)-the clodinafop-propargyl. The weeding composition of the invention obtained based on a proper mixing ratio can be processed into conventional formulations of pesticide such as wettable powder, suspension concentrate, water dispersible granules and the like by adopting a modern pesticide preparation processing technology, and the weeding composition can be used for controlling gramineous weeds and broadleaf weeds in fields such as cleavers, lithospermum arvense, alisma rhizome, common vetch, chickweed, capsella, silene jenisseensis willd, chorispora tenella, pennsylvania bittercress, stellaria alsine grimm, polygonum convolvulus, salvia alopecurus aequalis, wild oat, animated oat, brachiaria, crabgrass, barnyard grass, goosegrass, Italian ryegrass, goosefoot, common annual bluegrass, setaria glauca, green bristlegrass herb, Johnson grass and the like. The weeding composition has obvious control effect compared with the mode of only using the compound (A) or the compound (B) and significantly reduces application dosage of each active substance; and the weeding composition has great ecological and environmental protection significance.