579 results about "Ferulic acid" patented technology

The invention relates to a degradable filter element and a preparation method thereof, and belongs to the technical field of PLA filter elements. The degradable filter element is arranged between an atomizing element shell and a metal heating wire, a plurality of capillary holes are formed in the degradable filter element and used for being filled with tobacco tar, and the degradable filter element is prepared from a degradable composite material through melt spinning. The degradable composite material comprises the following components: polylactic acid and modified silicon dioxide, wherein the modified silicon dioxide is prepared by taking nano silicon dioxide, a silane coupling agent, ferulic acid, amino acid and copper chloride as raw materials, the silicon dioxide is organically modified, so that the dispersity of the silicon dioxide in a polylactic acid system is improved, the interface binding force of the silicon dioxide is improved, and the mechanical property of a polylactic acid material is improved; various antibacterial components are introduced, so that the antibacterial property of the polylactic acid material can be effectively improved.

The invention discloses feruloyl esterase fermentation preparation and application of feruloyl esterase in polyester plastic degradation, and belongs to the field of environmental science. According to the method, feruloyl esterase FAE4 derived from Anerocephalum thermophilum is prepared through fermentation, and is compounded with polyester degrading enzymes which are different in source and are used for degrading polyester plastic oligomers, so that a compound reaction system which remarkably improves the degradation efficiency of poly (butylene adipate-co-terephthalate) or poly (butylene adipate-co-terephthalate) (PBAT) and the content proportion of a final product is obtained; good industrial prospects are realized.

The invention relates to the field of microorganisms, and discloses an Aspergillus tubingensis strain and application thereof in preparation of meal seasonings, the Aspergillus tubingensis strain is not subjected to mutagenesis and acclimatization and has the capacity of high yield of glutaminase, feruloyl esterase, glucosidase, pectinase and protease, and when the Aspergillus tubingensis strain is applied to soy sauce brewing, the yield of soy sauce is increased. The high-value utilization of the meal raw materials can be realized, the amino acid content in the soy sauce is increased, and the flavor and nutritional value of the soy sauce are enhanced.

The invention belongs to the technical field of synthetic biology, and particularly relates to a method for artificially synthesizing vanillin by taking glucose as a substrate. According to the method, escherichia coli transformed bacteria transformed with a ferulic acid efficient synthesis module TTF, a ferulic acid synthesis vanillin function module AEL and a ferulic acid synthesis vanillin function module ODR are utilized, and the vanillin is prepared through fermentation with glucose as a substrate. The engineering bacterium constructed by the invention can efficiently synthesize and prepare natural vanillin, has a relatively prominent yield effect, and shows a relatively good application prospect. The microbial fermentation method is low in production cost, mild in preparation condition and low in preparation cost, and the prepared vanillin belongs to natural vanillin and can better meet the requirements of the fields of food, medicines, cosmetics and the like on natural vanillin, so that the method has better technical popularization and application significance.

The invention provides a cronobacter malonate strain and application thereof, the strain is preserved in China Center for Type Culture Collection on January 15, 2025, the preservation date is January 15, 2025, and the preservation number is CCTCC M 2025146. The strain has excellent capability of producing 4-vinyl guaiacol, and the yield in a sorghum juice fermentation culture medium is 2.70 + / -0.15 mg / L; meanwhile, the strain can resist ferulic acid and has good capability of converting ferulic acid to generate 4-vinyl guaiacol, and when 0.1% (w / w) of ferulic acid is added into a fermentation culture medium, the content of 4-vinyl guaiacol reaches 2.68 + / -0.04 g / L. The strain is applied to production of Maotai-flavor liquor, the content of 4-ethyl guaiacol in raw liquor reaches 3.20 + / -0.26 mg / L and is remarkably increased by 223.23% (P is smaller than 0.01) compared with the content of 4-ethyl guaiacol without the strain, the Maotai flavor is prominent through sensory evaluation, the flower, fruit and burnt flavor is obvious, and the strain has important significance on improvement of the health quality of the liquor.

The invention relates to natural plant hair dye, which comprises a bean extract, a lucid ganoderma extract, an astragalus extract, a polygonum multiflorum extract, vegetable fat, erythritol, L-proline, ferulic acid and gamma-aminobutyric acid, does not contain phenylenediamine, resorcinol, ammonium hydroxide, a vaccinium bracteatum thunb leaf extract and other components at all, and does not need to additionally add a thickening agent, a care agent and an alkalizing agent. The hair dye is good in hair dyeing effect, safe, reliable and non-irritant.

The invention belongs to the technical field of functional food processing, and particularly relates to a method for regulating peptide flavor and digesting characteristics by utilizing casein peptide and polyphenol self-assembly. According to the research, the casein peptide with good GABA-T inhibitory activity is taken as a raw material, and ferulic acid is added to be self-assembled with the casein peptide, so that the bitter taste of the casein peptide is reduced, and the GABA-T inhibitory activity of the casein peptide is improved in an in-vitro digestion stage. Compared with a traditional zymolyte debitterizing method, the method has the advantages that the biological activity of peptide is reserved, no toxic chemical reagent is introduced or added, and the method is safer, more efficient, more environmentally friendly and more sustainable. The method can be widely applied to functional food or health care products.

The invention belongs to the field of tobacco gene engineering, and particularly relates to application of a ferulic acid synthetic gene NtKAT2.2 in tobacco. The nucleotide sequence of the tobacco ferulic acid synthetic gene NtKAT2.2 is as shown in SEQ ID No. 1 (sequence identifier number 1). The expression quantity of the NtKAT2.2 gene is in negative correlation with the content of ferulic acid in tobacco. Preliminary research results show that the NtKAT2.2 gene is highly related to the content of the ferulic acid in tobacco, and after the gene is silenced, the content of the ferulic acid in a tobacco strain after gene silencing can be obviously increased. Based on the result, the inventor believes that on the basis of further deep research on the gene, a good technical foundation is expected to be laid for tobacco quality improvement and further new variety cultivation.

The invention discloses a lactobacillus mucus fermentation strain and application thereof, the lactobacillus mucus fermentation strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on February 21, 2025, and the preservation number is GDMCCNO: 65936. The feruloyl esterase is separated and screened from white sour soup in Kailing in southeast of Guizhou province, has feruloyl esterase activity, and shows good blood fat reducing and fatty liver treating capabilities in a HepG2 high-fat cell model and a high-fat zebra fish model. The compound has a good prospect in the development of products for preventing or treating hyperlipidemia and fatty liver and relieving obesity.

The invention discloses a ferulic acid-ectoine supramolecular eutectic as well as a preparation method and application thereof, and relates to the technical field of supramolecular eutectic preparation. The ferulic acid-ectoine supramolecular eutectic comprises ferulic acid and ectoine, and the molar ratio of the ferulic acid to the ectoine is 1: (1-3). The invention provides a ferulic acid-Ectoine supramolecular eutectic as well as a preparation method and application thereof, the ferulic acid-Ectoine supramolecular eutectic is prepared by adopting a mechanical grinding method or a solvent method, and the prepared ferulic acid-Ectoine supramolecular eutectic can improve the solubility of ferulic acid by 3.8 times or more, and can obtain excellent synergistic antioxidant and soothing effects.

The invention belongs to the technical field of microorganisms, and relates to a plant lactobacillus NXU-F1 strain and application thereof. The classification name of the bacterial strain is lactobacillus plantarum, the bacterial strain is preserved in Guangdong Microbial Culture Collection Center on June 20, 2025, and the preservation number of the bacterial strain is GDMCC No.66563. The invention also discloses a preparation method of the bacterial strain. The plant lactobacillus is strong in growth capability, has good adaptive capacity to environment, and can obviously increase the content of antioxidant substances when being applied to plant matrix fermentation. The lycium barbarum and grape compound fermented beverage is prepared by fermenting the lycium barbarum and grape compound fermented beverage, the content of polyphenol, flavone, polysaccharide and procyanidine in the beverage is obviously increased, and the increase of resveratrol and ferulic acid is particularly obvious; meanwhile, ester compounds are increased and aldehyde substances are reduced, so that the beverage is endowed with strong longan fruity flavor, and the flavor of harmonious sour and sweet taste and lingering aftertaste is formed.

The invention belongs to the technical field of quality control of traditional Chinese medicines, and particularly relates to a method for establishing a fingerprint spectrum of kidney-tonifying and anti-aging tablets. The establishment method of the fingerprint spectrum of the kidney-tonifying anti-aging tablet comprises the following steps: mixing the kidney-tonifying anti-aging tablet and a methanol aqueous solution, and carrying out ultrasonic treatment to obtain a test solution; reference substances in the mixed reference substance solution comprise ferulic acid, hyperoside, quercitrin, hesperidin, rosmarinic acid and salvianolic acid B; and respectively carrying out UHPLC detection on the test solution and the mixed reference substance solution, and introducing obtained chromatograms into fingerprint spectrum software for processing to obtain the fingerprint spectrum of the kidney-tonifying and anti-aging tablets. The pretreatment method is simple, and the characteristic components of the kidney-tonifying and anti-aging tablet are completely reserved; the ultra-high performance liquid chromatography is adopted, so that the precision is high, the reproducibility is good, and the stability is good; according to the fingerprint spectrum obtained by the invention, 15 common peaks are determined, so that the overall characteristics of the kidney-tonifying and anti-aging tablet can be reflected, and the quality of the kidney-tonifying and anti-aging tablet can be effectively monitored.

The present invention discloses a preparation method of a bio-based bisphenol, a bio-based epoxy resin, a preparation method thereof, and an application thereof, belonging to the field of polymer materials. The epoxy resin is obtained by condensing cheap and readily available ferulic acid, a bio-sourced diamine, and epichlorohydrin as raw materials. The epoxy resin has a simple curing process with a curing agent, high hardness, a high glass transition temperature, good heat resistance, strong toughness, and excellent mechanical properties.

The invention relates to the technical field of cosmetics, in particular to preparation and application of a cyclodextrin-coated azelaic acid oil-control skin-care product. Comprising the following steps: S1, adding deionized water into a mixer, adding a cyclodextrin-azelaic acid inclusion compound, nicotinamide and allantoin, mixing to obtain a water phase, mixing glycerol, caprylic / capric triglyceride, polydimethylsiloxane, polysorbate-80 and tocopheryl acetate to obtain an oil phase, slowly adding the oil phase into the water phase, and shearing and emulsifying to obtain a nanoemulsion; and S2, cooling the nano-emulsion, adding carbomer while stirring, continuously cooling to room temperature, sequentially adding phenoxyethanol, ferulic acid, menthol and essence, adjusting the pH value of the emulsion with triethanolamine, homogenizing, defoaming, disinfecting and filling. By adjusting the proportion of the components in the formula, the skin feeling of the product can be further optimized, so that the product is more comfortable and not greasy, and a lasting oil control effect is provided.

The invention relates to the technical field of rice bran processing, and discloses a rice bran degreasing and nutrition retention edible process synergistic treatment method which comprises the following steps: (a) in an NADES-aqueous solution medium, adding an immobilized catalyst A, and hydrolyzing ferulate bonds and phospholipids in rice bran at low temperature; (b) carrying out intermediate dehydration on the slurry obtained in the step (a) to reduce the water activity; (c) adding an immobilized catalyst B into the dehydrated slurry in the step (b), carrying out esterification reaction, and grafting free ferulic acid generated by hydrolysis to glyceride in situ; and (d) carrying out solid-liquid separation on the slurry obtained in the step (c) to obtain finished oil and finished powder. According to the method, an NADES medium is adopted to replace an organic solvent, the whole process is carried out at low temperature, degradation of heat-sensitive nutrient substances is avoided, and resource utilization of ferulic acid is achieved. The prepared functional rice bran oil is rich in feruloyl glyceride, total tocopherol and oryzanol, and is low in acid value and peroxide value.

The application discloses a squalane-based nanolipid carrier loaded with ethyl ferulate and a preparation method and application thereof. The squalane-based nanolipid carrier loaded with ethyl ferulate comprises the following components in percentage by weight: squalane 4-8%; solid oil 4-8%; ethyl ferulate 0.1-1.2%; non-ionic surfactant 1.5-5.5%; alcohol solvent 0.3-0.8%; and deionized water. The squalane-based nanolipid carrier loaded with ethyl ferulate can well load ferulate lipids, and the embedding rate is 90-97%. The particle size of the squalane-based nanolipid carrier loaded with ethyl ferulate is 100-200 nm, and the dispersion index is 0.22-0.26. The squalane-based nanolipid carrier loaded with ethyl ferulate has the advantages of simple preparation method, good particle size, dispersibility, transdermal efficiency and stability.

The invention discloses a method for inducing a regenerated plant based on a small spike stalk of bamboo-stalk, and relates to the technical field of regenerated plants.The method comprises the steps that the small spike of flowering bamboo-stalk is taken, disinfected, washed with sterile water and placed on sterile filter paper to absorb moisture, and dissected to obtain the small spike stalk as an explant; the small spike stalks are inoculated into a callus induction culture medium and cultured for 1-2 weeks under the dark condition, callus is formed through induction, and the induction culture medium comprises an MS culture medium, 2, 4-D, KT, ferulic acid, aspartic acid, Yb, an amino acid mixture and cane sugar; the callus is inoculated into a differential culture medium and cultured under the illumination condition, adventitious buds are differentiated, and the rooting culture medium comprises a 1 / 2 MS culture medium, IBA, NAA, ferric citrate, beta-salicylic acid and cane sugar. According to the method disclosed by the invention, a callus induction system starting from reproductive tissues of the bamboo-stalk bamboo is established, regenerated plants are successfully obtained, and a technical means is provided for rapid propagation of bamboo-stalk bamboo seedlings.

The invention relates to the technical field of disposable tableware, and discloses a material for paper tableware and a preparation method thereof.The preparation method comprises the steps that sugarcane fibers and bamboo fibers are pretreated to obtain a fiber mixture, and a treatment solution is collected to serve as a byproduct sugar solution; carrying out enzymolysis treatment on the fiber mixture to realize microfibrillation and release ferulic acid to obtain microfibrillated fiber pulp; fermenting and dispersing by using a byproduct sugar solution to prepare bacterial cellulose nano pulp; mixing the microfibrillated fiber pulp with bacterial cellulose nano-pulp, and carrying out enzymatic crosslinking to obtain crosslinked fiber pulp; and adding a modified sizing agent into the cross-linked fiber pulp, blending into pulp, and carrying out molding, curing, hot pressing and drying treatment to obtain the material for the paper tableware. The mechanical strength, the water resistance, the oil resistance and the thermal stability of the paper tableware material are remarkably improved, and the waste liquid load is effectively reduced.

The invention relates to the technical field of exosomes, and particularly discloses a cosmetic anti-aging mesenchymal stem cell exosome composition, which comprises a therapeutically effective amount of mesenchymal stem cell-derived exosome, the functional carrier comprises a transdermal absorption enhancer and an antioxidant stabilizer; at least one synergistic active component selected from the group consisting of small molecule peptides, plant-derived antioxidants, hyaluronic acid or derivatives thereof; the small molecule peptide is one of oligopeptide-1 and palmitoyl tripeptide-5; the plant source antioxidant is one of resveratrol and ferulic acid; wherein the exosome and the synergistic active component are compounded through a phospholipid bilayer fusion technology; through a phospholipid bilayer fusion technology, the mesenchymal stem cell exosome and a synergistic active component are directionally compounded, an exosome membrane structure is used as a natural carrier, the active component is effectively prevented from being degraded, and meanwhile, the bioavailability is improved by utilizing the targeting property of the exosome membrane structure.

The invention discloses a tetrandrine ferulate and its application in the preparation of a medicament for treating pulmonary fibrosis. The pharmacological effects of tetrandrine and ferulic acid, as well as those after salification, are studied. It is found that both have an anti-pulmonary fibrosis effect, and the two can be used in combination to synergistically exert a more excellent anti-pulmonary fibrosis efficacy. At the same time, the solubility of tetrandrine can also be increased. This has not been reported in existing literature. The tetrandrine ferulate of the present invention has lower cytotoxicity, and the cell viability of normal human liver cells LO-2 and human embryonic fibroblasts MRC-5 is higher than that of tetrandrine, which alleviates the burden of liver and lungs to a certain extent and has good safety.

The invention relates to the technical field of high polymer materials, in particular to an anti-aging thread gluing material and a preparation process thereof. Comprising the following steps: preparation of aluminum-doped zinc oxide, tannic acid modified aluminum-doped zinc oxide, ferulic acid modified nylon fiber and modified nylon master batch preparation of the thread gluing. According to the invention, groups of tannic acid and aluminum ions and zinc ions of aluminum-doped zinc oxide are driven by heat energy of a reflux reaction to simultaneously generate chelation-dehydration condensation to form a five-membered ring chelate, so that a compact coating layer is formed on the surface, and the coating layer is simple in component and has very stable chemical bonds; the ultraviolet light stability of aluminum-doped zinc oxide is enhanced, the layer can generate hydrogen bond / pi-pi interaction with nylon amido bonds, the interface bonding strength is improved, the particle size is kept at the level of no filter pressure fluctuation in the spinning process, and the volume resistivity of nylon fibers is reduced.

The invention provides a donkey-hide gelatin solid beverage and a preparation method thereof, the preparation method comprises the following steps: adding 8 times by mass of water into rhizoma polygonati, Chinese wolfberry, astragalus membranaceus, codonopsis pilosula, red dates, angelica sinensis and orange peel, soaking for 0.5 h, extracting for 2 h for the first time, adding 6 times by mass of water for extracting for 1.5 h for the second time, combining filtrates, and concentrating until the relative density is 1.23 g / cm < 3 > to obtain a water extraction concentrated solution; mixing the water extraction concentrated solution with maltodextrin, and drying to obtain an extract; and adding auxiliary materials into the extract and the donkey-hide gelatin, and molding to obtain the donkey-hide gelatin solid beverage. In the extraction step, proper soaking time, water adding amount and extraction time are combined, so that the ferulic acid content, the total polysaccharide content, the hesperidin content and the calycosin-7-glucoside content in the concentrated solution are all higher. And on the basis, subsequent drying and forming are carried out, so that the final donkey-hide gelatin solid beverage also has relatively high content of the components, and the content in each batch of products is stable.

The invention discloses application of salvianolic acid A in preparation of a medicine for preventing and treating vulnerable plaques of advanced atherosclerosis. The invention also discloses application of the salvianolic acid A combined with ferulic acid or the salt thereof in preparation of drugs for preventing and treating advanced atherosclerosis vulnerable plaques. Researches find that salvianolic acid A can promote apoptosis of senescent vascular smooth muscle cells and has a Senlytics effect; the salvianolic acid A stabilizes vulnerable atherosclerotic plaques by reducing the load of senescent vascular smooth muscle cells; the salvianolic acid A is in non-covalent binding with ASP54, ALA55, LYS58, ASP93, ASP102 and LYS112 of the HSP90AA1 to play a role in inhibition, and the ASP93 has the greatest contribution to binding free energy. The research of the invention is of great significance to the development and application of drugs with anti-aging effect for stabilizing advanced atherosclerotic vulnerable plaques.

The invention belongs to the technical field of skin repair, and particularly relates to a natural skin repair composition which is prepared from isosorbide dimethyl ether, hydroxypropyl tetrahydropyrantriol, ferulic acid, a dandelion extract, a chamomile extract, sodium hyaluronate, Ectoine, squalane, tartaric acid and water, and a specific preparation method is provided. According to the skin repairing composition, the defects of an existing skin repairing composition are overcome, the isosorbide dimethyl ether is used for permeation, the hydroxypropyl tetrahydropyrantriol is brought into a corium layer and an epidermal layer, the three-dimensional hyaluronic acid repairing and protecting effect of internally promoting external coating is formed in cooperation with the ectoine, the sodium hyaluronate and the squalane, and the excellent skin repairing effect is achieved.

The present invention relates to a recombinant microorganism comprising a heterologous nucleic acid sequence coding for a 4-coumaroyl-CoA ligase (4CL), a heterologous nucleic acid sequence coding for a coumaroyl-CoA 3-hydroxylase (CCoA3H) and a heterologous nucleic acid sequence coding for an acyl-coenzyme A thioesterase. The invention additionally relates to a recombinant microorganism comprising a heterologous nucleic acid sequence coding for a caffeic acid O-methyltransferase. It also relates to the use of these microorganisms for producing ferulic acid and / or caffeic acid and to methods for producing caffeic acid and / or ferulic acid using said microorganisms.

The invention belongs to the technical field of biological carriers, and particularly relates to a preparation method and application of an exosome ferulic acid nano-composite. The invention provides a way for preparing ferulic acid nano-particles by using exosomes, and particularly relates to a preparation process for forming a nano-composite by using ferulic acid and stress yeast exosomes generated by stimulation. The preparation method of the exosome ferulic acid nano-composite provided by the invention has the advantages of high safety, low immunological rejection, high cell fusion degree, high entrapment rate and good stability. Besides, the selected stress yeast exosome can effectively protect and enhance the anti-inflammatory and anti-oxidation effects of ferulic acid, so that the prepared exosome ferulic acid nano-composite has relatively strong anti-inflammatory, anti-oxidation and repair effects, and has a good application prospect in the field of cosmetics or medicines.

The present invention relates to a recombinant organism or microorganism having a decreased pool of crotonic acid compared to the organism or microorganism from which it is derived due to at least one of: (i) an increased conversion of crotonyl-CoA into butyryl-CoA; and / or an increased conversion of butyryl-CoA into butyric acid; (ii) an increased conversion of crotonyl-CoA into 3-hydroxybutyryl-CoA; and / or an increased conversion of 3-hydroxybutyryl-CoA into 3-hydroxybutyric acid; (iii) an increased conversion of crotonic acid into crotonyl-CoA; (iv) an increased conversion of crotonyl-[acyl-carrier protein] into butyryl [acyl-carrier-protein]; (v) a decreased conversion of crotonyl-CoA into crotonic acid; and / or (vi) a decreased conversion of crotonyl-[acyl-carrier protein] into crotonic acid. Moreover, the present invention relates to the use of such a recombinant organism or microorganism for the production of alkenes with the enzyme ferulic acid decarboxylase. Further, the present invention relates to a method for the production of isobutene or butadiene by culturing such a recombinant organism or microorganism in a suitable culture medium under suitable conditions.

The invention discloses a pharmaceutical composition, which comprises chlorogenic acid, ferulic acid and a zeolite imidazole framework material, the mass ratio of chlorogenic acid to ferulic acid is 4: 1-1: 4, and the zeolite imidazole framework material is a Zn-based zeolite imidazole framework material. In the composition, the compatibility of chlorogenic acid and ferulic acid can synergistically regulate the proliferation, migration and apoptosis of keratinocytes, and restore the physiological function imbalance of human skin keratinocytes caused by EGFRIs. The ZIF can efficiently carry chlorogenic acid and ferulic acid together, the nanoscale particle size of the ZIF is beneficial to permeation of the medicine into the skin, and hydroxyl on the surface of the ZIF and ceramide in the skin are subjected to hydrogen-bond interaction to retain the medicine at the focus position. The composition preparation disclosed by the invention is good in moisturizing performance and simple and convenient to use, and can be used for effectively treating skin toxic reactions such as pustule type rash, skin dryness and itching, perionychomycosis, abnormal hair regeneration and the like caused by EGFRIs.

The invention discloses hydrogel for promoting healing of diabetic wounds through cooperation of ganoderan-ferulic acid nanoparticles and rhEGF and a preparation method of the hydrogel. Belongs to the field of biomedical materials, and particularly relates to hydrogel for promoting healing of diabetic wounds and a preparation method thereof. The problem that healing of diabetic wounds is difficult due to the fact that the wounds are in the inflammation period for a long time is solved. The preparation method comprises the following steps: firstly, connecting ferulic acid to ganoderan through a chemical bond, then preparing nanoparticles through an anti-solvent precipitation method, and loading the nanoparticles and growth factors into temperature-sensitive hydrogel to obtain the CPP (at) GFNPs (at) EGF hydrogel. The hydrogel disclosed by the invention is used for regulating polarization of M1 type macrophages to M2 type macrophages, relieving inflammatory response at wounds, inhibiting bacterial growth and promoting formation of new vessels so as to promote healing of diabetic wounds. The preparation process of the hydrogel is simple to operate and convenient to use, and the hydrogel has good research, development and utilization prospects.

The invention discloses a construction process of high-yield vanillin chlamys farreri, and belongs to the technical field of vanillin production, which comprises the following steps: with ferulic acid as a substrate, vanillin is generated through conversion of a metabolic pathway, and the ferulic acid is an easily available natural compound and widely exists in plant cell walls. According to a definite vanillin synthesis route and a cofactor circulation route, amycolatopsis which can metabolize toxins and is low in vanillin degradation rate is selected as a chassis, and gene modification is performed on a strain by integrating vanillin synthetase of vanilla, so that efficient one-step synthesis of vanillin by the amycolatopsis is realized.