3 results about "Trichosanthin" patented technology

This invention discloses the application of plant extract saponins in aphid control and an aphidicide, belonging to the field of pesticides. This invention provides a novel plant-derived pesticide with aphid-killing activity, contributing to reducing aphid resistance and the use of chemical pesticides. Furthermore, the aphidicide prepared from plant extract saponins exhibits comparable aphid-killing efficacy to other commercial aphidicides, demonstrating broad development prospects and application value. It is an effective and feasible new aphidicide. Simultaneously, plant extract saponins are widely available and have a simple preparation process, which helps reduce the cost of aphidicides and facilitates widespread adoption.

The invention belongs to the technical field of biotechnology, traditional Chinese medicine modernization and functional food, and provides a trichosanthes root protease hydrolysate, a preparation method and application thereof.The preparation method adopts phosphate buffer solution low-temperature extraction to help maintain protein activity and reduce denaturation loss, ammonium sulfate fractional precipitation and blue agarose gel affinity chromatography are combined, and the content of trichosanthes root protease hydrolysate is increased. The target protein can be efficiently purified, and a high-purity reaction substrate is provided for subsequent enzymolysis, so that the uniformity and activity stability of a final product are improved. In the two-step composite enzymolysis step, directional shearing of protein is realized by accurately controlling the ratio of alkaline protease to trypsin, the action pH value, the temperature and the time, so that not only can the generation of small molecular peptide fragments be promoted, but also the molecular weight distribution of the product can be improved, the proportion of high-activity components less than 1kDa is remarkably increased, and the yield of the product is improved. The solubility and the oral absorption efficiency are enhanced. The preparation method disclosed by the invention is mild in condition, clear in operation parameter, good in repeatability and easy for large-scale amplification.

This invention discloses a triptolide sustained-release injection, its preparation method, and its application, belonging to the field of pharmaceutical technology. The sustained-release injection is composed of triptolide-polylactic acid-glycolic acid copolymer nanoparticles and hyaluronic acid hydrogel. The triptolide-polylactic acid-glycolic acid copolymer nanoparticles are prepared by a double emulsion-solvent evaporation method, with an encapsulation efficiency of 60.58±0.91% and a drug loading of 1.16±0.13%. The nanoparticles are further encapsulated in the hyaluronic acid hydrogel to form a composite formulation with good injectability. This invention also provides a method for establishing a collagen-induced arthritis (CIA) model in mice. By administering the drug via local intra-articular injection, a dual sustained-release effect is achieved, significantly reducing the systemic toxicity of triptolide, alleviating paw swelling, improving knee joint pathological changes, regulating serum inflammatory factor levels, and reducing liver and spleen damage in the model mice. This triptolide sustained-release injection has broad prospects for application in the preparation of drugs for treating rheumatoid arthritis.