24 results about "Trypsin inhibitor" patented technology

The invention belongs to the technical field of biological medicine, and relates to an oral peptide drug delivery system based on a soybean trypsin inhibitor and core-shell hydrogel, the oral peptide drug delivery system comprises therapeutic polypeptide, KTI and a core-shell structure gel matrix, and the problems that an existing oral peptide delivery system is passively protected and drug burst release is remarkable are solved. A chitosan / sodium tripolyphosphate ionic cross-linked network forms an inner core, a sodium alginate / calcium ion'egg box 'structure cross-linked network forms a shell, and therapeutic polypeptides such as liraglutide and KTI are jointly embedded. Through the pH response characteristic and sequential release design of the core-shell structure, KTI is preferentially and rapidly released in the intestinal environment to inhibit local protease activity, then therapeutic polypeptide is slowly released, and active protection and synergistic interaction are achieved. The invention further provides a specific preparation method and parameters. The polypeptide has the advantages of stable structure in a gastric acid environment, targeted release in intestinal tracts, high encapsulation efficiency, capability of avoiding burst release and the like, and provides a new strategy for efficient delivery of oral polypeptide drugs.

A method comprising the steps of: (a) assessing an expression level of at least one protein, selected from the list consisting of: hemoglobin subunit beta and inter-alpha-trypsin inhibitor light chain, in a sample from a subject, and (b) using the expression level to determine whether the subject has endometriosis.

The invention relates to a soybean clear water feed capable of remarkably promoting growth of stichopus japonicus and a preparation method and application of the soybean clear water feed, and the soybean clear water feed comprises the following components in parts by mass: 20-40 parts of lactobacillus fermented soybean clear water and 100 parts of stichopus japonicus basal feed. The lactobacillus fermented soybean clear water is obtained by adding lactobacillus bulgaricus into the soybean clear water for fermentation; the preparation method comprises the following steps: fully mixing a stichopus japonicus basal feed, crushing, filtering and screening by using a 100-mesh screen, adding lactobacillus fermented soybean clear water, fully and uniformly mixing, granulating and drying. The soybean clear water stichopus japonicus feed is suitable for stichopus japonicus culture modes such as indoor ponds, outdoor ponds and outdoor net cages. By using the bean clear water, the environmental pollution is reduced, and the feed production cost is reduced; meanwhile, the nutrition is rich, macromolecular proteins, phytic acid, trypsin inhibitors and other anti-nutritional factors in the soybean clear water are fully degraded in the lactic acid bacteria fermentation process, the intestinal flora of the stichopus japonicus can be optimized, the feed utilization rate is increased, the weight gain of the stichopus japonicus is promoted, and the antioxidant capacity of the stichopus japonicus is enhanced.

The present disclosure relates to the field of genetic engineering or enzyme engineering, and in particular to an application of MmBBK2 in preparation of trypsin and chymotrypsin inhibitors. The MmBBK2 has the amino acid sequence shown in SEQ ID NO. 1 or SEQ ID NO. 2. The present disclosure clarifies for the first time that MmBBK2 in mulberry leaves has both trypsin and chymotrypsin inhibitory activity, and reveals its physical and chemical properties. The MmBBK2 has good application prospects in preparing trypsin and chymotrypsin inhibitors. On the basis of knowing its physical and chemical properties, its activity may be accordingly eliminated, promoting the development and utilization of mulberry leaf resources in animal feed, providing new perspectives and ideas for the development and utilization of mulberry leaves in animal feed and health food, and improving economic benefits of mulberry resources.

A method comprising the steps of: (a) assessing an expression level of at least one protein, selected from Table 1, 2 or 3 in a sample from a subject, where in the at least one protein may be selected from the list comprising: Beta-Ala-His dipeptidase, Apolipoprotein L1, Methanethiol oxidase, Vitamin K-dependent protein S, von Willebrand factor, Plasminogen, Selenoprotein P, Protein disulfide-isomerase A6 and Inter-alpha-trypsin inhibitor heavy chain H3, and (b) using the expression level to determine whether the subject has endometriosis.

An application of inter-alpha-trypsin inhibitor heavy chain 1 (ITIH1) in preparing a drug for treating hepatocellular carcinoma (HCC) is provided, relating to the field of biomedical technologies. The drug includes the ITIH1 or a reagent for increasing an expression of the ITIH1. The reagent includes a small molecule compound, an antibody drug, a protein, a nucleic acid molecule, a polypeptide, a lipid, a carbohydrate or a combination thereof. It proves a new function of ITIH1, that is, ITIH1 can act as a tumor suppressor in the progression of HCC. Moreover, in preclinical models (mouse model, human-like organ and human-like tissue xenotransplantation), it is confirmed that the purified recombinant ITIH1 (r-ITIH1) protein can be used to treat HCC. Based on the function of ITIH1, it can provide a target for developing drugs to alleviate and / or treat HCC.

The invention discloses a Chinese style functional pastry and a preparation method thereof. Firstly, white kidney beans are induced to germinate, endogenous phytase is activated to reduce the content of resistance factors such as phytic acid and lectin, and the activity of an alpha-amylase inhibitor is protected by spraying a soybean trypsin inhibitor. And then mixing the germinated white kidney beans with the aqueous extracts of astragalus membranaceus and poria cocos, inoculating saccharomycetes and lactobacillus plantarum for fermentation to generate flavor substances and active components, and consuming small molecular sugar to maintain the low GI characteristic. Finally, puerarin powder and the like are added into the white kidney bean paste and the Chinese yam paste to prepare dough, the dough wraps the functional stuffing prepared from the chia seed powder, the Chinese wolfberry fruit powder and the red date and longan paste, and the finished product is obtained through forming, steaming and rose dew incense. The method effectively solves the contradiction between alpha-amylase inhibitor loss and anti-nutritional factor degradation in germination, improves the bioavailability, flavor level and intestinal health regulation function of the product, and realizes the synergistic interaction of nutrition enrichment, blood sugar management and multiple nourishing regulation.

The invention discloses a trypsin inhibitor oligopeptide conjugate and application thereof. According to the invention, three analogues for coupling trypsin inhibition rings (TIL) of BBI peptides with short peptides are designed, so that the biological activity of the peptides is enhanced. Generally speaking, all the analogues show significant antibacterial activity improvement, and the inhibitory activity of the analogues with the '-GG-' connexon to cancer cell proliferation is specifically enhanced. Meanwhile, all analogues show relatively low hemolytic activity under the highest test concentration. In conclusion, the coupling obviously enhances the biological function, especially the antibacterial activity. More interesting, the existence of the residue linker (-GG-) promotes the improvement of the biological activities to a great extent, especially in the aspect of inhibiting cancer cell proliferation. The discovery of oligopeptide coupling in the research provides powerful evidence for potential new application of the oligopeptide coupling, and paves a way for future development of the oligopeptide coupling as a novel antibacterial and anticancer agent.

A lily and white kidney bean chewable tablet and its preparation method belong to the field of chewable tablet processing technology. The lily and white kidney bean chewable tablet comprises the following components in parts by weight: 20 parts dried lily powder, 25 parts white kidney bean powder, 10 parts maltodextrin, 5 parts sorbitol, 1 part magnesium stearate, 0.4 parts citric acid, and an appropriate amount of purified water. This invention disrupts part of the starch structure in lily, making its mucilage more easily absorbed by the human body. This component has an auxiliary effect on regulating blood lipids. The processing of white kidney beans avoids the phytohemagglutinin and trypsin inhibitors they contain, which hinder protein absorption and irritate the gastrointestinal tract. It also maximizes the retention of dietary fiber and B vitamins in white kidney beans, helping to control blood sugar and lower blood lipids.

The invention belongs to the fields of biotechnology, protein engineering and plant protection science, and particularly relates to an antagonistic protein mutant for cultivating a sweet potato composite virus disease (SPVD) resistant plant and application of the antagonistic protein mutant. The key pathogenic factor of the SPVD is the RNase3 protein coded by the sweet potato chlorotic stunt virus (SPCSV), and the RNase3 protein is used as an RNA silence suppressor (RSS) to destroy host immunity. The invention provides an SPCSV-RNase3 antagonistic protein mutant, which is derived from a natural antagonistic protein, namely a sweet potato trypsin inhibitor (IbSPLTI-a). According to the present invention, a compound structure model (such as construction through AlphaFold2) of wild type IbSPLTI-a and RNase3 is analyzed, and the IbSPLTI-a is modified by using a protein directed evolution strategy, particularly by using a hotspot amino acid scanning and protein surface design strategy, such that the high-activity inhibition mutant (such as IbSPLTI-a-m0805) is successfully created and screened; in-vivo and in-vitro function verification shows that the binding affinity of the mutant (such as IbSPLTI-a-m0805) and SPCSV-RNase 3 is remarkably enhanced, and the mutant shows a virus accumulation inhibition effect superior to that of a wild type IbSPLTI-a. The invention also provides a nucleic acid sequence for coding the mutant, a plant expression vector containing the sequence, and a method for culturing an anti-SPVD transgenic plant (especially sweet potato) by using the mutant. The invention provides a core gene resource and a technical path for genetic improvement of SPVD and development of a novel antiviral protein preparation.

This disclosure relates to the use of inter-alpha-trypsin inhibitor heavy chain 2 (ITIH2) as a biomarker for wound healing, specifically for distinguishing healing from non-healing wounds. Also provided are methods for treating, predicting responsiveness to a wound treatment, and prognosing wound progression based on biomarker levels.

Preparation of Factor XA Derivatives.SOLUTION: The present disclosure provides methods for large-scale manufacturing of fXa derivative proteins to obtain high yields of high purity protein products. The method can comprise adding a detergent to a sample comprising a polynucleotide construct encoding a protein and purifying the protein via soybean trypsin inhibitor (STI) - based affinity chromatography, ion exchange and mixed mode chromatography, and hydrophobic interaction.SELECTED DRAWING: None

The invention provides a pulping control method and device, a storage medium and a food processer, the method is applied to the field of cooking, and the method comprises the following steps: when the food processer enters a first cooking stage, controlling a heating device to heat a pulping material in a cooking cavity of the food processer according to a first heating parameter, the preparation method comprises the following steps: preparing a pulping material, controlling a stirring device to stir-fry the pulping material according to a first stirring parameter, so that the pulping material is cured, that is, saponin and a trypsin inhibitor are completely inactivated, and then controlling a water injection device to inject water into the cured pulping material, so as to obtain a water-material mixture; after the water is added, the soybean milk making materials do not need to be boiled at a high temperature again to be cooked thoroughly, the second cooking stage can be directly carried out, the stirring device is controlled according to the second stirring parameter to crush the water-material mixture so that the water-material mixture can form soybean milk, and finally, after the soybean milk making is finished, the safe normal-temperature soybean milk with completely inactivated trypsin statin can be directly obtained.

The invention discloses a soybean clear water fancy carp feed capable of remarkably improving the growth speed of fancy carps as well as a preparation method and application of the soybean clear water fancy carp feed. The feed comprises 20-40 parts of soybean clear water fermented by lactic acid bacteria and 100 parts of basal feed, the basal feed comprises fish meal, flour, spirulina powder, fish oil, compound vitamins, mineral premix, yeast extract, astaxanthin and carotene. The lactobacillus fermented soybean clear water is obtained by adding lactobacillus bulgaricus into the soybean clear water and fermenting under the conditions that the pH is 6.0 and the temperature is 37 DEG C. The preparation method comprises the following steps: fully and uniformly mixing the basal feed and the soybean clear water, puffing, granulating and drying to prepare the fancy carp feed. By adding the lactic acid fermented soybean clear water, the soybean product wastewater can be recycled, and soluble protein, amino acid and trace elements rich in the soybean clear water, especially phytic acid, trypsin inhibitor and other antinutritional factors after lactic acid bacteria fermentation are fully degraded, so that the digestibility of the feed can be remarkably improved; the oxidation resistance and the growth speed of the fancy carp are improved.

The invention discloses a trypsin inhibitory peptide with antibacterial activity and application of the trypsin inhibitory peptide. Drug-resistant bacteria cause millions of infections in the world every year, and development of novel antibacterial agents becomes an important task. The invention discloses a bifunctional polypeptide with strong trypsin inhibitory activity and antibacterial activity. The peptide is also a novel derivative (BBI) of Borman-Burkholderia trypsin inhibitor polypeptide; the derivative peptide not only has strong bactericidal ability to two drug-resistant strains of Escherichia coli and methicillin-resistant staphylococcus, but also has excellent performance in safety evaluation of erythrocyte dissolution activity and anti-proliferative activity to human epithelial cuticle forming cells. Besides, the antibacterial activity of the novel bifunctional peptide is not obviously interfered under the conditions of salt ions, serum and trypsin, and the novel bifunctional peptide shows certain resistance to the salt ions, the serum and the trypsin. Therefore, the peptides have clinical development potential as novel clinical antibacterial preparations.

The invention belongs to the field of food, and particularly relates to a preparation process of high-purity low-antinutritional-factor soybean protein. Specifically, the invention provides a method for extracting soybean protein or a method for reducing the phytic acid content of soybean protein, and the method comprises the following steps: (1) equally dividing a soybean raw material into n batches for extraction at 15-35 DEG C, extracting for m times in each batch, n being a positive integer greater than or equal to 2, and m being a positive integer greater than or equal to 3; (2) mixing the soybean protein-containing protein slurry obtained by the first extraction and separation of each batch; wherein in the first batch of extraction, the soybean raw material is extracted by using water, and in the second batch of extraction and each batch of extraction, the soybean raw material is extracted by using a liquid phase obtained by the previous batch of extraction. The invention also provides a method for extracting soybean protein. The method provided by the invention can effectively reduce the content of anti-nutritional factors such as trypsin inhibitors and phytic acid in the protein powder, improve the digestibility of protein and the utilization rate of mineral substances, and optimize the nutritional efficacy of the product.

The application discloses a kit for individualized diagnosis of M protein of a multiple myeloma patient and application thereof, relates to the technical field of multiple myeloma related M protein detection, and the detection reagent comprises Kunitz type soybean trypsin inhibitor and 1% formic acid solution. The Kunitz type soybean trypsin inhibitor is used as a standard substance, mass spectrometry technology is used to individualize detection of the accurate molecular weight and peak intensity of M protein complete light chains in blood, and the content of the M protein complete light chains in the patient is accurately quantified according to the amount of the added standard substance, so that a new technical method and support are provided for detection of multiple myeloma M protein microresiduals in a treatment process.

The invention relates to the technical field of plant polypeptide, and discloses a low-temperature refrigeration method for preparing soybean protein peptide through enzymolysis and application of the low-temperature refrigeration method. Soybean protein is subjected to enzymolysis by simultaneously fixing alkaline protease and bromelain with sodium alginate, then enzymolysis is performed by respectively fixing pepsase and trypsin to prepare soybean protein peptide, and the soybean protein peptide can be added to pig feed. According to the low-temperature refrigeration method, active ingredients in soybeans can be effectively reserved, meanwhile, the inhibition effect of a soybean trypsin inhibitor on trypsin is prevented, and meanwhile generation of bitter peptides is reduced; the obtained soybean protein peptide has the effect of inhibiting swine pathogenic bacteria such as streptococcus suis and clostridium perfringens. The compound can be used as a medicine for treating bacterial infection of pigs, replaces traditional antibiotics, avoids the problems of drug resistance and food safety, and realizes healthy breeding and benefit improvement.

This invention provides a method for screening, separating, and identifying trypsin inhibitors, belonging to the field of biomedical technology. The invention first uses colorimetric sensor technology to rapidly identify samples with trypsin-inhibiting activity. Then, using affinity adsorption technology combined with liquid chromatography-mass spectrometry (LC-MS), specific components in the samples with trypsin-inhibiting activity can be identified. Specifically, trypsin is immobilized on Tp-Pa to form a TRY@Tp-Pa affinity chromatography carrier material. The extract to be screened is incubated with the affinity chromatography carrier material, and after removing the unadsorbed liquid, methanol is used to elute the eluent, which contains the bioactive compounds retained on TRY@Tp-Pa. These compounds are the potential active ingredients, and LC-MS is then used to identify the components. The method provided by this invention can efficiently and accurately detect trypsin activity and screen inhibitors, with high screening efficiency and a low false positive rate.

The present invention relates to a method for determining disease activity of myasthenia gravis in a patient, comprising determining the inter-alpha-trypsin inhibitor heavy chain H3 (ITIH3) level in an in vitro sample from the patient. Furthermore, the invention relates to a kit for use in such method comprising means for determining the ITIH3 level in a sample and instructions for carrying out the method.

The present application relates to genetic engineering and relates to a trichosanthin trypsin inhibitor gene SsBBI and SsBBI3 gene and its encoded product and application SsBBI3 The nucleotide sequence of the gene is shown as SEQ ID NO. 1, the amino acid sequence of the encoded product is shown as SEQ ID NO. 2, and the trichosanthin trypsin inhibitor protein SsBBI3 is obtained by cloning, amplification and expression in engineering bacteria of the trichosanthin trypsin inhibitor gene SsBBI3 The protein is modified from the trichosanthin trypsin inhibitor protein SsBBI, and the modification method is to cut off the intramembrane region and the transmembrane region of SsBBI and retain the extramembrane region. The nucleotide sequence of the SsBBI protein is shown as SEQ ID NO. 3, and the amino acid sequence of the encoded product is shown as SEQ ID NO. 4. This trichosanthin trypsin inhibitor has the effect of significantly inhibiting tumor cell proliferation at a low dose, and has good application prospect.

The invention belongs to the technical field of food processing, and discloses an ultrasonic-assisted detoxified bean product and a preparation method thereof.According to the method, an ultrasonic technology is combined with traditional heat treatment and enzyme treatment, toxic components in the bean product are efficiently removed, the nutritional value of the bean product is reserved, the heat treatment process is optimized through the ultrasonic technology, and the heat treatment efficiency is improved. A more efficient detoxification effect is realized at a lower temperature, the speed and the effect of the enzymolysis reaction are remarkably improved, and the loss of nutritional ingredients at a high temperature in a traditional detoxification method is avoided. Experimental results show that according to the method, the decrease rate of the trypsin inhibitor content exceeds 90%, the saponin residue is lower than 0.1 mg / g, the protein retention rate is larger than or equal to 80%, and the in-vitro protein digestibility is improved.

The invention provides a trypsin inhibitor and application thereof, and relates to the technical field of molecular biology. According to the invention, it is found for the first time that zinc pyrithione, ketoconazole and / or selenium disulfide have an inhibition effect on trypsin activity, and new functional components which are convenient to obtain are provided for the trypsin inhibitor. The invention provides three compositions containing zinc hyaluronate, the zinc hyaluronate is respectively compounded with zinc pyrithione, ketoconazole or selenium disulfide according to a specific mass ratio, and it is found for the first time that the zinc hyaluronate and the zinc pyrithione, ketoconazole or selenium disulfide show the activity of synergistically inhibiting trypsin. The composition provided by the invention is simple to obtain, can be continuously compounded with other functional active components or auxiliary material components to obtain commercial products, and has a wide application prospect.