63 results about "Trypsin inhibitor" patented technology

The invention discloses protein-based nanoparticles as well as a preparation method and application thereof. The protein-based nanoparticles have a core-shell structure, the core is zein particles loaded with active drugs, and the shell comprises a trypsin inhibitor. The preparation method of the protein-based nanoparticles comprises the following steps: 1) preparing a zein dispersion liquid loaded with an active drug and a shell material aqueous solution; and 2) slowly adding the zein dispersion liquid loaded with the active drug into the shell material aqueous solution in a stirring state, and then carrying out rotary evaporation and freeze drying. The protein-based nano-particle has an excellent gastrointestinal digestion resisting effect, various hydrophobic active drugs can be effectively delivered to a lower digestive system, the stability and slow release efficiency of the loaded drugs are remarkably enhanced, and the protein-based nano-particle is good in biocompatibility, safe, non-toxic, simple in preparation process and suitable for industrial production. The method is suitable for large-scale industrial production and application.

The invention relates to the technical field of industrial microorganisms, and particularly discloses bacillus velezensis phb02 and application thereof. The invention provides bacillus velezensis phb02. The bacillus velezensis phb02 has stress resistance, can tolerate 2M salt and well grow at 42 DEG C, and the degradation rate of the bacillus velezensis phb02 to a trypsin inhibitor can reach 59.34%; the produced protease also has the characteristics of salt resistance and high temperature resistance, and the degradation rate of the protease on a trypsin inhibitor can still reach 35% under the salt concentration of 3M. The strain can be used for treating soybeans or soybean derived products through synergistic fermentation or in a mode of preparing an enzyme preparation, degrading a trypsin inhibitor in raw materials and improving the utilization rate of raw material protein, can be used for producing fermented condiments such as soy sauce and the like, remarkably improves the ammonia nitrogen level of the products and improves the quality of the products.

The invention discloses a two-channel colorimetric fluorescent composite probe for detecting trypsin as well as a detection method and application. The two-channel colorimetric fluorescent composite probe comprises orange red carbon quantum dots and cytochrome C in a volume ratio of 1.5: 1. On one hand, the two-channel colorimetric fluorescent composite probe disclosed by the invention can conveniently and sensitively realize detection of trypsin in serum and urine samples, and has important significance for researching the relationship between the trypsin and the occurrence and development of diseases; on the other hand, the existence of the trypsin inhibitor can cause the change of a colorimetric fluorescence signal, so that the preliminary screening of the novel trypsin inhibitor is carried out, and a simple, convenient and rapid new method is provided for the discovery of the novel trypsin inhibitor. The detection method is expected to realize rapid diagnosis of trypsin-related diseases and rapid screening of novel trypsin inhibitors.

The invention discloses a method for preparing soybean milk with low antinutritional factors by using an ultrasonic technology, and belongs to the field of food processing. In order to solve the problems that the content of antinutritional factors in the existing soybean milk is relatively high and the absorption of human bodies is influenced, the invention provides the preparation method of the soybean milk with low antinutritional factors. The method mainly comprises the steps of soaking, grinding, ultrasonic treatment, high-pressure homogenization, heating and the like. Anti-nutritional factors are effectively removed from the soybean milk obtained through ultrasonic treatment, the content of a trypsin inhibitor is remarkably reduced, and the protein content and the in-vitro digestibility are both improved. The soybean milk subjected to ultrasonic treatment is rich in nutrition, easy to absorb by a human body and low in antinutritional factor content, and has a good application prospect in the field.

The invention discloses a reagent for removing cell agglomerates and fragments in a single-cell suspension and application of the reagent, and relates to the technical field of single-cell suspension preparation. The invention relates to a reagent for removing cell agglomerates and fragments in a single-cell suspension. The reagent comprises a Percoll stock solution, 0.85% w / v of NaCl, 0.5% w / v of EDTA (Ethylene Diamine Tetraacetic Acid), 0.5-2.5 mg / mL of ADH-1 trifluoroacetate and 5.0-8.5 mg / mL of a trypsin inhibitor. According to the reagent for removing the cell agglomerates and fragments in the single-cell suspension and the application of the reagent, the cell agglomerates and fragments generated in the process of dissociating animal tissues into the single-cell suspension can be remarkably removed, so that the single-cell suspension with high-activity cells and minimum fragments is obtained, and a foundation is laid for subsequent single-cell sequencing.

The invention belongs to the technical field of biological medicine, and relates to an oral peptide drug delivery system based on a soybean trypsin inhibitor and core-shell hydrogel, the oral peptide drug delivery system comprises therapeutic polypeptide, KTI and a core-shell structure gel matrix, and the problems that an existing oral peptide delivery system is passively protected and drug burst release is remarkable are solved. A chitosan / sodium tripolyphosphate ionic cross-linked network forms an inner core, a sodium alginate / calcium ion'egg box 'structure cross-linked network forms a shell, and therapeutic polypeptides such as liraglutide and KTI are jointly embedded. Through the pH response characteristic and sequential release design of the core-shell structure, KTI is preferentially and rapidly released in the intestinal environment to inhibit local protease activity, then therapeutic polypeptide is slowly released, and active protection and synergistic interaction are achieved. The invention further provides a specific preparation method and parameters. The polypeptide has the advantages of stable structure in a gastric acid environment, targeted release in intestinal tracts, high encapsulation efficiency, capability of avoiding burst release and the like, and provides a new strategy for efficient delivery of oral polypeptide drugs.

A method comprising the steps of: (a) assessing an expression level of at least one protein, selected from the list consisting of: hemoglobin subunit beta and inter-alpha-trypsin inhibitor light chain, in a sample from a subject, and (b) using the expression level to determine whether the subject has endometriosis.

The present invention discloses a dual-channel colorimetric fluorescence composite probe for detecting trypsin, a detection method and an application thereof. The dual-channel colorimetric fluorescence composite probe comprises orange-red carbon quantum dots and cytochrome C with a volume ratio of 1.5:1. The dual-channel colorimetric fluorescence composite probe of the present invention can, on the one hand, conveniently and sensitively detect trypsin in samples including serum and urine samples, which is of great significance for studying the relationship between trypsin and the occurrence and development of diseases; on the other hand, the presence of a trypsin inhibitor can cause changes in the colorimetric fluorescence signal, thereby enabling the preliminary screening of novel trypsin inhibitors, providing a simple and rapid new method for the discovery of novel trypsin inhibitors. This detection method is expected to achieve the rapid diagnosis of trypsin-related diseases and the rapid screening of novel trypsin inhibitors.

The invention belongs to the technical field of pesticide compounding, and particularly relates to a pesticide based on a soybean trypsin inhibitor and application, the effective components of the pesticide comprise a pesticide and a synergist, the pesticide is azadirachtin, and the synergist is the soybean trypsin inhibitor. In addition, the invention also provides an agricultural composition for preventing and treating lepidoptera pests, the effective components comprise azadirachtin and a soybean trypsin inhibitor, the effective concentration of the azadirachtin is 0.1289-0.3585 mg / L, and the effective concentration of the soybean trypsin inhibitor is 0.1 mM. The soybean trypsin inhibitor has the advantages that the insecticidal effect is obviously enhanced, the pesticide dosage is effectively reduced, the fatality rate of spodoptera frugiperda is effectively increased, the high insecticidal effect can be achieved under the condition of low-concentration azadirachtin, the chemical pesticide dosage is reduced, the drug resistance risk is relieved, and the pesticide reducing and synergistic requirements of green agriculture are met.

The present invention relates to the technical field of industrial microorganisms, and specifically discloses a strain of Bacillus amyloliquefaciens phb03 and its application. The present invention provides a strain of Bacillus amyloliquefaciens phb03, which has stress resistance, can tolerate 2M salt, grows well at 42°C, and can achieve a degradation rate of 42.26% for trypsin inhibitors; the protease produced by the strain also has the characteristics of salt and high temperature resistance, and can still maintain 57% relative enzyme activity after being stored at 50°C for 180 minutes, and can still achieve a degradation rate of 40% for trypsin inhibitors at a salt concentration of 4M. The strain can process soybeans or soybean-derived products by means of coordinated fermentation or preparation of enzyme preparations, degrade trypsin inhibitors in raw materials, improve the utilization rate of raw material protein, can be used in the production of fermented condiments such as soy sauce, significantly increase the amino acid nitrogen and total acid content in the product, and improve product quality.

The invention relates to a soybean clear water feed capable of remarkably promoting growth of stichopus japonicus and a preparation method and application of the soybean clear water feed, and the soybean clear water feed comprises the following components in parts by mass: 20-40 parts of lactobacillus fermented soybean clear water and 100 parts of stichopus japonicus basal feed. The lactobacillus fermented soybean clear water is obtained by adding lactobacillus bulgaricus into the soybean clear water for fermentation; the preparation method comprises the following steps: fully mixing a stichopus japonicus basal feed, crushing, filtering and screening by using a 100-mesh screen, adding lactobacillus fermented soybean clear water, fully and uniformly mixing, granulating and drying. The soybean clear water stichopus japonicus feed is suitable for stichopus japonicus culture modes such as indoor ponds, outdoor ponds and outdoor net cages. By using the bean clear water, the environmental pollution is reduced, and the feed production cost is reduced; meanwhile, the nutrition is rich, macromolecular proteins, phytic acid, trypsin inhibitors and other anti-nutritional factors in the soybean clear water are fully degraded in the lactic acid bacteria fermentation process, the intestinal flora of the stichopus japonicus can be optimized, the feed utilization rate is increased, the weight gain of the stichopus japonicus is promoted, and the antioxidant capacity of the stichopus japonicus is enhanced.

The present invention discloses a reagent for removing cell clumps and fragments in a single cell suspension and its application, and relates to the technical field of single cell suspension preparation. A reagent for removing cell clumps and fragments in a single cell suspension comprises Percoll stock solution, 0.85% w / v NaCl, 0.5% w / v EDTA, 0.5-2.5 mg / mL ADH‑1 trifluoroacetate and 5.0-8.5 mg / mL trypsin inhibitor. The present invention adopts the above-mentioned reagent for removing cell clumps and fragments in a single cell suspension and its application, which can significantly remove the cell clumps and fragments generated during the dissociation of animal tissue into a single cell suspension, thereby obtaining a single cell suspension with high viability cells and minimal fragments, laying the foundation for subsequent single cell sequencing.

The present disclosure relates to the field of genetic engineering or enzyme engineering, and in particular to an application of MmBBK2 in preparation of trypsin and chymotrypsin inhibitors. The MmBBK2 has the amino acid sequence shown in SEQ ID NO. 1 or SEQ ID NO. 2. The present disclosure clarifies for the first time that MmBBK2 in mulberry leaves has both trypsin and chymotrypsin inhibitory activity, and reveals its physical and chemical properties. The MmBBK2 has good application prospects in preparing trypsin and chymotrypsin inhibitors. On the basis of knowing its physical and chemical properties, its activity may be accordingly eliminated, promoting the development and utilization of mulberry leaf resources in animal feed, providing new perspectives and ideas for the development and utilization of mulberry leaves in animal feed and health food, and improving economic benefits of mulberry resources.

This invention discloses a rice trypsin inhibitor, OsBBTI13, its encoding gene, and its applications. The amino acid sequence of the rice trypsin inhibitor OsBBTI13 is shown in SEQ ID No. 1, and its encoding gene... OsBBTI13 The nucleotide sequence is shown in SEQ ID No. 2. The Japanese white rice... OsBBTI13 After gene interference expression, the transformed plants exhibited a shortened growth period under both long-day and short-day conditions, with a significant increase in yield under short-day conditions. Furthermore, the transformed plants showed better adaptability to low temperatures, exhibiting an earlier growth period at 20°C. Therefore, silencing gene expression... OsBBTI13 Genes can be used to breed new early-maturing, high-yielding, and cold-resistant rice varieties, which have good market prospects and economic value.

The present disclosure provides a method for large-scale manufacturing of fXa-derived proteins to obtain a high yield of a high-purity protein product. The method may include adding a detergent to a sample containing a polynucleotide construct encoding the protein, and purifying the protein by affinity chromatography, ion exchange and mixed mode chromatography and hydrophobic interaction based on soybean trypsin inhibitor (STI).

A method comprising the steps of: (a) assessing an expression level of at least one protein, selected from Table 1, 2 or 3 in a sample from a subject, where in the at least one protein may be selected from the list comprising: Beta-Ala-His dipeptidase, Apolipoprotein L1, Methanethiol oxidase, Vitamin K-dependent protein S, von Willebrand factor, Plasminogen, Selenoprotein P, Protein disulfide-isomerase A6 and Inter-alpha-trypsin inhibitor heavy chain H3, and (b) using the expression level to determine whether the subject has endometriosis.

An application of inter-alpha-trypsin inhibitor heavy chain 1 (ITIH1) in preparing a drug for treating hepatocellular carcinoma (HCC) is provided, relating to the field of biomedical technologies. The drug includes the ITIH1 or a reagent for increasing an expression of the ITIH1. The reagent includes a small molecule compound, an antibody drug, a protein, a nucleic acid molecule, a polypeptide, a lipid, a carbohydrate or a combination thereof. It proves a new function of ITIH1, that is, ITIH1 can act as a tumor suppressor in the progression of HCC. Moreover, in preclinical models (mouse model, human-like organ and human-like tissue xenotransplantation), it is confirmed that the purified recombinant ITIH1 (r-ITIH1) protein can be used to treat HCC. Based on the function of ITIH1, it can provide a target for developing drugs to alleviate and / or treat HCC.

The invention belongs to the technical field of trypsin, and particularly relates to a trypsin affinity material as well as a preparation method and application thereof. The preparation method of the trypsin affinity material comprises the following steps: preparing magnetic nanoparticles Fe3O4 (at) PDA by taking Fe3O4 magnetic particles and dopamine hydrochloride as raw materials; a buckwheat trypsin inhibitor is modified, active amino acid is introduced to the N end of the buckwheat trypsin inhibitor, the optimized buckwheat trypsin inhibitor eBTI is obtained, and the active amino acid comprises any one of cysteine, lysine and arginine; and preparing the Fe3O4 (at) PDA (at) eBTI. The buckwheat trypsin inhibitor is modified by analyzing the structure and sequence of the buckwheat trypsin inhibitor, and active groups such as amino and sulfydryl are introduced to realize directional immobilization of the buckwheat trypsin inhibitor at specific sites, so that a trypsin affinity material is provided. The material can be used for purifying animal trypsin.

The invention discloses a Chinese style functional pastry and a preparation method thereof. Firstly, white kidney beans are induced to germinate, endogenous phytase is activated to reduce the content of resistance factors such as phytic acid and lectin, and the activity of an alpha-amylase inhibitor is protected by spraying a soybean trypsin inhibitor. And then mixing the germinated white kidney beans with the aqueous extracts of astragalus membranaceus and poria cocos, inoculating saccharomycetes and lactobacillus plantarum for fermentation to generate flavor substances and active components, and consuming small molecular sugar to maintain the low GI characteristic. Finally, puerarin powder and the like are added into the white kidney bean paste and the Chinese yam paste to prepare dough, the dough wraps the functional stuffing prepared from the chia seed powder, the Chinese wolfberry fruit powder and the red date and longan paste, and the finished product is obtained through forming, steaming and rose dew incense. The method effectively solves the contradiction between alpha-amylase inhibitor loss and anti-nutritional factor degradation in germination, improves the bioavailability, flavor level and intestinal health regulation function of the product, and realizes the synergistic interaction of nutrition enrichment, blood sugar management and multiple nourishing regulation.

The application discloses a competitive binding method for evaluating the interaction between trypsin and flavonoids, wherein the interaction between different flavonoids and trypsin is analyzed by means of electrospray ionization mass spectrometry (ESI-MS) in a competitive mode of mass spectrometry; the parameter R can be introduced to compare the relative strength of the affinity, to judge whether the different flavonoids compete with each other in the binding to trypsin, and to further infer whether the binding sites of the different flavonoids on trypsin are the same; the method is simple, fast and high in accuracy, and can provide a feasible scheme for rapidly screening trypsin inhibitors.

The invention discloses a trypsin inhibitor oligopeptide conjugate and application thereof. According to the invention, three analogues for coupling trypsin inhibition rings (TIL) of BBI peptides with short peptides are designed, so that the biological activity of the peptides is enhanced. Generally speaking, all the analogues show significant antibacterial activity improvement, and the inhibitory activity of the analogues with the '-GG-' connexon to cancer cell proliferation is specifically enhanced. Meanwhile, all analogues show relatively low hemolytic activity under the highest test concentration. In conclusion, the coupling obviously enhances the biological function, especially the antibacterial activity. More interesting, the existence of the residue linker (-GG-) promotes the improvement of the biological activities to a great extent, especially in the aspect of inhibiting cancer cell proliferation. The discovery of oligopeptide coupling in the research provides powerful evidence for potential new application of the oligopeptide coupling, and paves a way for future development of the oligopeptide coupling as a novel antibacterial and anticancer agent.

A lily and white kidney bean chewable tablet and its preparation method belong to the field of chewable tablet processing technology. The lily and white kidney bean chewable tablet comprises the following components in parts by weight: 20 parts dried lily powder, 25 parts white kidney bean powder, 10 parts maltodextrin, 5 parts sorbitol, 1 part magnesium stearate, 0.4 parts citric acid, and an appropriate amount of purified water. This invention disrupts part of the starch structure in lily, making its mucilage more easily absorbed by the human body. This component has an auxiliary effect on regulating blood lipids. The processing of white kidney beans avoids the phytohemagglutinin and trypsin inhibitors they contain, which hinder protein absorption and irritate the gastrointestinal tract. It also maximizes the retention of dietary fiber and B vitamins in white kidney beans, helping to control blood sugar and lower blood lipids.

The invention belongs to the fields of biotechnology, protein engineering and plant protection science, and particularly relates to an antagonistic protein mutant for cultivating a sweet potato composite virus disease (SPVD) resistant plant and application of the antagonistic protein mutant. The key pathogenic factor of the SPVD is the RNase3 protein coded by the sweet potato chlorotic stunt virus (SPCSV), and the RNase3 protein is used as an RNA silence suppressor (RSS) to destroy host immunity. The invention provides an SPCSV-RNase3 antagonistic protein mutant, which is derived from a natural antagonistic protein, namely a sweet potato trypsin inhibitor (IbSPLTI-a). According to the present invention, a compound structure model (such as construction through AlphaFold2) of wild type IbSPLTI-a and RNase3 is analyzed, and the IbSPLTI-a is modified by using a protein directed evolution strategy, particularly by using a hotspot amino acid scanning and protein surface design strategy, such that the high-activity inhibition mutant (such as IbSPLTI-a-m0805) is successfully created and screened; in-vivo and in-vitro function verification shows that the binding affinity of the mutant (such as IbSPLTI-a-m0805) and SPCSV-RNase 3 is remarkably enhanced, and the mutant shows a virus accumulation inhibition effect superior to that of a wild type IbSPLTI-a. The invention also provides a nucleic acid sequence for coding the mutant, a plant expression vector containing the sequence, and a method for culturing an anti-SPVD transgenic plant (especially sweet potato) by using the mutant. The invention provides a core gene resource and a technical path for genetic improvement of SPVD and development of a novel antiviral protein preparation.

This disclosure relates to the use of inter-alpha-trypsin inhibitor heavy chain 2 (ITIH2) as a biomarker for wound healing, specifically for distinguishing healing from non-healing wounds. Also provided are methods for treating, predicting responsiveness to a wound treatment, and prognosing wound progression based on biomarker levels.

Preparation of Factor XA Derivatives.SOLUTION: The present disclosure provides methods for large-scale manufacturing of fXa derivative proteins to obtain high yields of high purity protein products. The method can comprise adding a detergent to a sample comprising a polynucleotide construct encoding a protein and purifying the protein via soybean trypsin inhibitor (STI) - based affinity chromatography, ion exchange and mixed mode chromatography, and hydrophobic interaction.SELECTED DRAWING: None

The application belongs to the technical field of enzyme engineering, and particularly relates to a trypsin inhibitor degrading enzyme mutant and application thereof. The application provides a trypsin inhibitor degrading enzyme mutant, the SDP enzyme activity of which is increased by nearly 20% compared with that of the original protein, can tolerate a high-salt environment, and can still maintain about 90% of the activity after being treated at a 3M salt concentration for 24 hours; the degradation rate of the trypsin inhibitor can still reach nearly 50% at a 3M salt concentration, and can meet the application requirement of a high-salt scene such as food fermentation. The trypsin inhibitor degrading enzyme mutant can effectively degrade the trypsin inhibitor in soybean or soybean derivative products, improve the utilization rate of raw material protein, is suitable for the production of legume fermented products, significantly improves the ammonia nitrogen level of the products, and improves the product quality.

The invention provides a pulping control method and device, a storage medium and a food processer, the method is applied to the field of cooking, and the method comprises the following steps: when the food processer enters a first cooking stage, controlling a heating device to heat a pulping material in a cooking cavity of the food processer according to a first heating parameter, the preparation method comprises the following steps: preparing a pulping material, controlling a stirring device to stir-fry the pulping material according to a first stirring parameter, so that the pulping material is cured, that is, saponin and a trypsin inhibitor are completely inactivated, and then controlling a water injection device to inject water into the cured pulping material, so as to obtain a water-material mixture; after the water is added, the soybean milk making materials do not need to be boiled at a high temperature again to be cooked thoroughly, the second cooking stage can be directly carried out, the stirring device is controlled according to the second stirring parameter to crush the water-material mixture so that the water-material mixture can form soybean milk, and finally, after the soybean milk making is finished, the safe normal-temperature soybean milk with completely inactivated trypsin statin can be directly obtained.

The present invention relates to a culture medium comprising low glucose DMEM, L- glutamine, foetal bovine serum (FBS), antimicrobials and a trypsin inhibitor; the use of said culture medium for culturing mammalian cells and / or maintaining the viability of live tissue or tumour slices, optionally in a perfusion model; a method for 5 identifying a treatment for cancer utilising said culture medium; and a method for perfusion cell culture utilising said culture medium.

The application discloses a group of markers related to the phenotype of frequent acute exacerbation of chronic obstructive pulmonary disease (COPD) and application thereof. The markers include any one or a combination of at least two of inter-alpha-trypsin inhibitor heavy chain H3, lymphatic endothelial hyaluronan receptor 1, paraoxonase 1, alanine-glyoxylate transaminase, immunoglobulin kappa variable region 3D-11, fodrin 2, apolipoprotein F, dopamine beta-hydroxylase, muscle creatine kinase, FAM20C golgi-associated secretory pathway kinase or fat storage-inducing transmembrane protein 1. The application mines a group of nucleic acid / protein biomarkers which can be used for detecting and identifying the patient population of frequent acute exacerbation and non-frequent acute exacerbation phenotypes in the stable stage of COPD, can diagnose the two different patient populations of COPD, and can perform precise treatment and early warning.