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Aspergillus zryzae strain capable of highly producing neutral proteinase and fermentation method thereof on solid state substrate

A neutral protease and fermentation method technology, applied in the field of Aspergillus oryzae strain and its fermentation on solid substrate, can solve the problem that trypsin inhibitor and soybean lectin are also proteins in nature, and achieve simple fermentation process and easy raw materials. The effect of improving the yield, fermentation process and enzyme production performance

Inactive Publication Date: 2008-03-19
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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AI Technical Summary

Problems solved by technology

Trypsin inhibitors and soybean lectins are also proteins in nature. It has been reported that various proteases such as neutral and alkaline proteases can decompose anti-nutritional factors in plant proteins and partially inactivate them, but no successful use of these proteases has been reported. Aquafeed enzyme preparation, and can eliminate plant protein anti-nutritional factors such as trypsin inhibitor and soybean lectin in aquatic feed

Method used

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  • Aspergillus zryzae strain capable of highly producing neutral proteinase and fermentation method thereof on solid state substrate
  • Aspergillus zryzae strain capable of highly producing neutral proteinase and fermentation method thereof on solid state substrate
  • Aspergillus zryzae strain capable of highly producing neutral proteinase and fermentation method thereof on solid state substrate

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Experimental program
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Embodiment 1

[0072] 1. Bacteria culture:

[0073] 1. Slant strain culture: Inoculate the Aspergillus oryzae variant ZW-06# obtained by this technology on the slant of Potato Ru Dextrose Agar Medium (PDA), and cultivate it at a constant temperature of 28-30°C for 72-96 hours until plump yellow After the green spores, take them out and store them in a refrigerator at 4°C for 5-15 days. When in use, take it out and place it at room temperature for activation, then make a spore suspension (with a concentration of 107-108 pcs / ml) with sterile water, and set it aside for use.

[0074] 2. Solid seed culture: Mix 1.8kg of bran and 0.18kg of soybean meal, and then add 1% concentration of (NH 4 ) 2 SO 4 The solution was mixed thoroughly again to make a solid seed medium. The culture medium was sterilized at 121°C for 30 minutes, and after cooling, it was inserted into the spore suspension of slant strains by 1-5% (W / W), fully stirred evenly, and filled with 30 grams of water in each bottle of 50...

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Abstract

The present invention relates to aspergillus oryzae strain and a fermentation method on a solid cultivation medium. The aspergillus oryzae strain (Aspergillus oryza) ZW-06 of the high-producing neutral proteinase of the present invention is conserved in the China committee for culture collection of microorganisms on July 10th in 2006, and the collection number is CGMCC No.1754. The aspergillus oryzae strain of the present invention can highly produce the neutral proteinase, the produced neutral proteinase has high enzyme activity, and besides the depressant of the trypsase in the cake and meal feed material can be effectively degraded.

Description

technical field [0001] The invention relates to an aspergillus oryzae strain and a fermentation method on a solid substrate. Background technique [0002] As a biocatalyst, neutral protease has been widely used in food, brewing, medicine and other industries. The production of neutral protease also has a relatively long history of development. However, most of the neutral proteases produced in China are industrial grade at present, and the post-treatment of the fermentation products adopts the spray drying method, and some even use ammonium sulfate salting-out and other processes. A small amount of food-grade neutral protease is relatively expensive due to its complicated manufacturing process. At present, the neutral protease contained in the compound enzyme applied to livestock and poultry feed or the compound enzyme applied to aquatic feed basically adopts industrial grade neutral protease. Feed-grade high-activity neutral proteases developed for animal physiology and ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/62C12R1/69
Inventor 许尧兴李艳丽许少春李孝辉
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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