Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing ADD from phytosterols by microbial transformation and culture medium thereof

A technology for microbial transformation and phytosterol, which is applied to a method for phytosterol to be transformed into ADD by microorganisms and the field of culture medium used, which can solve the problems of no industrial value and the like

Inactive Publication Date: 2014-04-02
PEKING UNIV FOUNDER GRP CO LTD +2
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Calculated from an economic point of view, the above technical indicators show that it has no industrial value

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing ADD from phytosterols by microbial transformation and culture medium thereof
  • Method for producing ADD from phytosterols by microbial transformation and culture medium thereof
  • Method for producing ADD from phytosterols by microbial transformation and culture medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: Fermentation and extraction process of phytosterol biotransformation into ADD

[0059] ADD comes from mycobacterium culture fluid, and its cultivation, extraction and purification steps are as follows:

[0060] (1) Seed cultivation

[0061] The ratio and preparation method of the culture medium of the first-level seed bottle and the second-level seed tank are the same, and the composition of the liquid medium is shown in Table 1.

[0062] Table 1 Composition of seed medium (g / liter)

[0063]

[0064] When preparing, fully dissolve various raw materials according to the ratio, stir evenly, the natural pH value is 6.7±0.2, use 40% NaOH solution to adjust the pH value to 7.0, then heat up to 118-121°C, steam sterilize for 30 minutes, and cool down To the culture temperature to be inoculated.

[0065] Use the inclined surface to inoculate directly, scrape all the bacterial lawn from one inclined surface, and inoculate it into a first-level seed bottle (2...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for producing ADD (androstadienedione) from phytosterols by microbial transformation and a culture medium thereof. The fermentation medium contains the following substances which can dissolve or disperse in water: 0.1 g / L to 100 g / L of glucose, 10 g / L to 100 g / L of soybean powders, 0.5 g / L to 10 g / L of potassium dihydrogen phosphate, 0.1 g / L to 10 g / L of calcium carbonate, 0.001 g / L to 0.01 g / L of ferrous sulfate, 0.0001 g / L to 0.001 g / L of manganese chloride, and 10 g / L to 100 g / L of phytosterols. The pH of the fermentation medium is 5.0 to 8.0; and the fermentation medium is free of phytosterol cosolvents or dispersants. By the method and culture medium provided by the invention, the rate of transformation from phytosterols to ADD is increased, the concentration of substrates is increased to increase the transformation efficiency, and the specificity of transformation is improved and the separation and purification process is simplified.

Description

technical field [0001] The invention relates to the field of pharmacy, and in particular, the invention relates to a method for converting phytosterols into ADD by microorganisms and the culture medium used. Background technique [0002] Androstadienedione (ADD) is an important intermediate for the synthesis of steroid hormone drugs, through which more than 10 clinically widely used drugs including dexamethasone, prednisolone, and betamethasone can be synthesized. hormone drugs. The chemical structural formula of ADD is shown in Formula I below. [0003] [0004] Formula I [0005] There are currently two methods for synthesizing androstadienedione: one is to use diosgenin as the starting material and obtain it through 11 steps of chemical synthesis. The second is to obtain ADD directly from phytosterols through microbial transformation. Among them, microbial transformation has the advantages of mild reaction conditions, energy saving and environmental protection. Whe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P15/00C12R1/32
Inventor 赵德刘省伟岳光何勇崴何晶
Owner PEKING UNIV FOUNDER GRP CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com