Agronomically elite soybeans with high beta-conglycinin content

Abstract

The invention overcomes the deficiencies of the art by providing an agronomically elite soybean plant with non-transgenic mutations of at least two of the glycinin subunits selected from the group consisting of Gy1, Gy2, Gy3, Gy4, and Gy5, such as conferring a Gy3 and Gy4 null phenotype and increased β-conglycinin content in seed. The invention also provides derivatives, and plant parts of these plants and uses thereof. Methods for marker assisted selection of soybean varieties comprising non-transgenic mutations conferring a reduced Gy1, Gy2, Gy3, Gy4, and Gy5 phenotype are also provided as part of the current invention. Methods for producing such plants that are further lipoxygenase and/or Kunitz Trypsin Inhibitor null and the plants produced thereby are also provided. The invention is significant in that soybeans from such plants are preferred dietary additives and provide important health benefits.

Description

BACKGROUND OF THE INVENTION [0001] This application claims the priority of U.S. Provisional Application Ser. No. 60 / 714,779, filed Sep. 7, 2005; and U.S. Provisional Application Ser. No. 60 / 722,493, filed Sep. 30, 2005; each of the disclosures of which are incorporated herein by reference.INCORPORATION BY REFERENCE OF SEQUENCE LISTING SUBMITTED ON A COMPACT DISC [0002] The Sequence Listing is submitted on one compact disc (Copy 1), together with a duplicate thereof (Copy 2), each created on Sep. 7, 2006, and each containing one—79 kb file entitled “MSUT015U.S.APP.TXT.” The material contained on the compact disc is specifically incorporated herein by reference. [0003] 1. Field of the Invention [0004] The present invention relates generally to the field of plant breeding and molecular biology. In particular, the invention relates to agronomically elite soybean varieties with increased beta-conglycinin content and materials for making such plants. [0005] 2. Description of Related Art [...

AI Technical Summary

Benefits of technology

[0012] The current invention provides, in one embodiment, a plant of an agronomically elite soybean variety with an increased seed β-conglycinin content, comprising non-transgenic mutations providing a null phenotype of at least two of the glycinin subunits selected from the group consisting of Gy1, Gy2, Gy3, Gy4, and Gy5. An increased seed β-conglycinin content may be measured, for example, with respect to a plant of the same genotype as said plant but lacking the mutations. In specific embodiments, the non-transgenic mutations may confer a Gy3, and Gy4 null phenotype and increased seed β-conglycinin content. Thus, the plants of the current invention comprise, in one aspect, seeds with low glycinin content and high β-conglycinin content. In certain embodiments, the seed β-conglycinin content for plants of the invention is about or at least abou

Problems solved by technology

In the case of complex inheritance, such as with quantitative traits, including specifically glycinin content and yield, linkage will generally be much more difficult to discern.

RFLPs have the disadvantage of being labor-intensive in the typing stage, although this can be alleviated to some extent by multiplexing many of the tasks and reutilization of blots.

Identification of individuals that are genetically superior is difficult because genotypic value can be masked by confounding plant traits or environmental factors.

Therefore, the accurate identification of transgressive segregates or superior genotypes with the traits of interest is extremely difficult and its success is dependent on the plant breeder's ability to minimize the environmental variation affecting the expression of the quantitative character in the population.

For example, if a cross is made between c

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