27 results about "Insoluble protein" patented technology

To provide a method for removing abundant plant-derived soluble proteins from a plant-derived extract.SOLUTION: The present invention provides a method for removing a plant-derived soluble protein from a plant-derived extract, the method comprising the following steps: (1) a step for freezing and thawing a plant-derived extract to cause the insolubilization of a plant-derived soluble protein; and (2) a step for removing the insolubilized protein from the extract.SELECTED DRAWING: None

The invention relates to the technical field of biochemical engineering, in particular to a method for extracting insoluble protein powder from wheat bran. Comprising the following steps: S1, adding lecithin and octyl glycoside into water, stirring for 10-15 minutes, adding wheat bran powder, heating and stirring for 1-3 hours, filtering, washing, drying, adding into a eutectic solvent, heating and stirring for 2-4 hours, centrifuging, washing and drying to obtain pretreated wheat bran powder; s2, adding a complex microbial inoculant into the suspension, and treating for 6-8 hours; centrifuging to obtain supernate and precipitate; wherein the mass ratio of the suspension to the complex microbial inoculant is (60-70): (0.3-0.6); and S3, washing the precipitate, and freeze-drying to obtain the insoluble protein powder. The method is low in cost, green and safe, and the prepared insoluble protein powder is high in purity and suitable for industrial production.

The application discloses a controllable preparation method of high-activity earthworm protein peptide and belongs to the technical field of earthworm processing. The preparation method comprises the following steps: (1) adding amylase and cutinase to earthworm paste for hydrolysis; (2) filtering by using a filter; (3) hydrolyzing the filtrate by using a composite enzyme; (4) adding alkaline protease to the filter residue for hydrolysis, and then adding high-temperature protease for hydrolysis; (5) filtering the hydrolyzed paste, and then filtering and treating the paste by using a ceramic microfilter to obtain low-molecular-weight earthworm protein peptide; and (6) mixing the obtained earthworm water-soluble protein hydrolysate and the obtained earthworm insoluble protein polypeptide, and then concentrating and drying to obtain high-activity earthworm protein peptide. According to the application, the water-soluble protein and the water-insoluble protein in the earthworm are step-by-step differentially hydrolyzed, the protein resources in the earthworm are maximally utilized, the polypeptide / protein yield can be more than 85%, waste is avoided, and the activity of the obtained protein peptide is ensured.

Stable dispersions of encapsulated pigments suitable for use in food, cosmetic, pharmaceutical, or personal care products are described herein. Pigments encapsulated in a water-insoluble protein matrix, in particulate form, are homogeneously dispersed in a sol colloid formed by high-shear mixing between a solid fat and a liquid oil. Within the liquid oil, the solid fat's creaming tendency is believed to counteract the sedimentation tendency of the encapsulated pigment, resulting in a dispersions with improved stability.

The present application belongs to the technical field of biological pharmacy, and particularly relates to a method for extracting and purifying spinosad. The method for extracting and purifying spinosad from saccharopolyspora spinosa fermentation broth by combining enzymolysis and acid extraction reduces the amount of organic solvent; the membrane separation technology is used to purify the spinosad extract, the microfiltration and ultrafiltration are used to remove the bacterial bodies, insoluble proteins, colloid, pigments and other macromolecular impurities, and the nanofiltration is used to remove small molecular water-soluble impurities, so as to realize the purification of the extract; the two-phase extraction is used to realize the second purification of the spinosad; and the evaporation crystallization is used to realize the third purification of the spinosad. Compared with the prior art, the method for extracting and purifying spinosad has the advantages of simple process, high efficiency, environmental protection, low consumption, and the like.

The present disclosure provides a formulation for extracting a water-insoluble protein, as well as a kit and a method using the formulation to rapidly detect gluten allergen. The formulation includes a Tris buffer and an ionic liquid.

Stable dispersions of encapsulated pigments suitable for use in food, cosmetic, pharmaceutical, or personal care products are described herein. Pigments encapsulated in a water-insoluble protein matrix, in particulate form, are homogeneously dispersed in a sol colloid formed by high-shear mixing between a solid fat and a liquid oil. Within the liquid oil, the solid fat's creaming tendency is believed to counteract the sedimentation tendency of the encapsulated pigment, resulting in a dispersions with improved stability.

The present disclosure relates to rendering of animal material to usable products. Particularly, the disclosure relates to treatment of animal material by wet rendering including enzymatic hydrolysis. Herein, methods and arrangements for treating animal material are disclosed to yield products where distribution of soluble protein, insoluble protein and minerals is controlled.

To provide an instant soup composition or the like containing protein at a high concentration and having improved texture of soup.SOLUTION: A solid instant soup composition comprising (A) a particulate insoluble proteinaceous material and (B) water-soluble collagen, wherein a weight ratio (A: B) of a content of the (A) to a content of the (B) is 1:0.18 to 1:1.5, and a total protein amount is 40 to 75% by weight.SELECTED DRAWING: None

The present invention relates to the technical field of test kits, and specifically to a colloidal gold test kit and detection method for detecting poorly soluble proteins. The test kit includes a test card, a swab, a No. 1 centrifuge tube, a No. 2 centrifuge tube, a No. 3 centrifuge tube, and a pipette; the test card includes a sample pad, a conjugation pad, a test pad, an absorbent pad, and a base plate; the lower surface of one end of the sample pad is bonded to the upper surface of one end of the conjugation pad; the lower surface of the other end of the conjugation pad is bonded to the upper surface of one end of the test pad, and the upper surface of the other end of the test pad is bonded to the lower surface of one end of the absorbent pad; a C1q area, a detection line area, and a quality control line area are provided on the test pad; the conjugation pad contains a colloidal gold-labeled secondary antibody and a quality control primary antibody, the C1q area contains complement C1q, the detection line area contains a tertiary antibody, and the quality control line area contains a quality control secondary antibody. The colloidal gold test kit visually determines whether the target poorly soluble protein is present in the sample by whether the detection line area and the quality control line area develop color.

This method for producing an insoluble protein-containing powder product involves mixing a raw material containing an insoluble protein and a lipid with water, and subsequently subjecting the mixture to a spray drying treatment, wherein the insoluble protein-containing powder product in which dispersibility of the insoluble protein with poor dispersibility is improved is produced by adjusting the ratio of the lipid to the raw material containing the insoluble protein and the lipid and / or by adjusting the outlet temperature of a spray dryer in the spray drying treatment.

The application belongs to the technical field of protein purification, and discloses a protein purification method based on condensate, which comprises the following steps: firstly, constructing a target expression vector; secondly, expressing a fusion protein with an LplA label in E. coli; thirdly, ultrasonically crushing the E. coli, centrifuging the crushing liquid, and taking supernatant; fourthly, performing first temperature change, heating the supernatant, centrifuging and collecting the fusion protein after the fusion protein forms condensate, discarding the supernatant, and taking the precipitate; and fifthly, performing second temperature change, redissolving the precipitate obtained in the fourth step in a buffer at low temperature, removing insoluble proteins by centrifugation at low temperature, and taking the supernatant as the fusion protein with the LplA label after purification. The application connects the temperature-responsive purification label LplA which can form condensate to the target protein, and only needs to perform two temperature change treatments to quickly obtain the target protein with high purity, without the need of complex chromatography equipment and expensive fillers, and the application is suitable for large-scale production and purification of functional proteins.

The invention discloses an egg white protein thermal stability regulation and control method based on microenvironment reconstruction and application, and relates to the technical field of food processing. The method comprises the following steps: pre-treating egg white protein to remove water-insoluble protein so as to obtain water-soluble egg white protein; the preparation method comprises the following steps: diluting water-soluble egg white protein until the protein concentration is 0.5%-3% (w / v), then adjusting the pH value to 10.1-10.5, and heating for 20-40 minutes under the condition of 90-100 DEG C; cooling the solution, adjusting the pH to be neutral, and performing ultrafiltration concentration to obtain a neutral protein concentrated solution; or cooling the solution, adjusting the pH value to 4-6, centrifugally collecting the protein, adding a proper amount of pure water, adjusting the pH value to obtain a neutral protein concentrated solution, and carrying out freeze drying or spray drying to obtain the heat-stable nano-scale recombinant egg white protein with a food industry full bacteriocidal spectrum (70-135 DEG C). The microenvironment reconstruction treatment process is simple, low in cost and suitable for large-scale production, the heat stability of the egg white protein is still maintained at high temperature and high pressure (120 DEG C / 0.1 MPa), and the advantages in the aspects of cost reduction and environmental influence reduction are outstanding.

An opacity modifying agent in dry particulate form as an alternative t titanium dioxide is described herein. The opacity modifying agent may comprise a calcium salt encapsulated in a water-insoluble protein matrix via extrusion encapsulation, leading to the encapsulated calcium salt having higher opacity than that of the unencapsulated calcium salt. In embodiments, the opacity modifying agent may comprise calcium carbonate encapsulated in an alkaline rice protein extract having a whiteness approaching that of calcium carbonate. Processes for producing the opacity modifying agents, as well as their uses in food, cosmetic, or personal care products are also described herein.

The invention relates to the technical field of kits, in particular to a colloidal gold kit for detecting insoluble protein and a detection method. The kit comprises a detection card, a swab, a first centrifugal tube, a second centrifugal tube, a third centrifugal tube and a suction tube, the detection card comprises a sample pad, a combination pad, a test pad, an absorption pad and a bottom plate, wherein the lower surface of one end of the sample pad is attached to the upper surface of one end of the combination pad; the lower surface of the other end of the combination pad is attached to the upper surface of one end of the test pad, and the upper surface of the other end of the test pad is attached to the lower surface of one end of the absorption pad; a C1q region, a detection line region and a quality control line region are arranged on the test pad; the conjugate pad contains a colloidal gold labeled secondary antibody and a quality control primary antibody, the C1q region contains a complement C1q, the detection line region contains a third antibody, and the quality control line region contains a quality control secondary antibody. According to the colloidal gold detection kit, whether target insoluble protein exists in a sample or not is intuitively judged by detecting whether color development occurs in the detection line area and the quality control line area or not.

The present invention concerns a method for detecting apoptosis, wherein the method comprises the steps of: heating a non-purified test sample comprising a population of cells; and subsequently determining the level of a soluble biomarker protein in said heated test sample or in a soluble protein fraction thereof, or determining the level of an insoluble biomarker protein in an insoluble protein fraction of said heated test sample, wherein apoptosis is detected by an increase in the level of the soluble biomarker protein as compared to a control level, and / or by a decrease in the level of the insoluble biomarker protein as compared to a control level. Further disclosed is a method for identifying an apoptosis-inducing agent, a method of investigating apoptotic processes in a population of cells and a method of diagnosing an apoptotic-associated condition or disease in a subject, all using the above method.

The invention provides a method for detecting the content of protein in tobacco. The method comprises the following steps: 1) mixing a tobacco sample to be detected with a solution of an enzyme preparation, and reacting; (2) adding a dimethyl sulfoxide aqueous solution into a product obtained in the step (1), mixing, and extracting protein; the enzyme preparation comprises pectinase, cellulase and hemicellulase.The method has the advantages that soluble and insoluble proteins in tobacco leaves can be sufficiently extracted by the aid of the method, and the method is high in selectivity and sensitivity and high in accuracy of detection results.

The present invention concerns a method for detecting apoptosis, wherein the method comprises the steps of: heating a non-purified test sample comprising a population of cells; and subsequently determining the level of a soluble biomarker protein in said heated test sample or in a soluble protein fraction thereof, or determining the level of an insoluble biomarker protein in an insoluble protein fraction of said heated test sample, wherein apoptosis is detected by an increase in the level of the soluble biomarker protein as compared to a control level, and / or by a decrease in the level of the insoluble biomarker protein as compared to a control level.

The invention mainly relates to the technical field of microscopic infrared spectroscopy. The invention provides a method for rapidly analyzing trace insoluble protein particles in white spirit, and aims to rapidly identify and analyze insoluble protein particles with low content and small particle size in white spirit. The method mainly comprises the following steps: carrying out negative pressure suction filtration on white spirit through a silicon film, then naturally air-drying the white spirit, placing a slide on a placement table of a microscopic infrared spectrometer, adjusting and optimizing detection parameters, and focusing and splicing an image of the whole filter film; adding liquid nitrogen into the microscopic infrared spectrometer, and carrying out spot scanning, line scanning and surface scanning on the distributed visible foreign matters to collect spectral data; and deducting the background of the collected spectral data to obtain a spectral result, comparing the spectral result with a standard spectrum or a self-established spectrum library, and judging the source of the insoluble protein particles in the wine sample in combination with a sample source.

The present invention provides a method for producing bread that can control the degree of bread-making ability and the quality of the final bread based on the signal area values ​​of sodium lauryl sulfate-soluble proteins and sodium lauryl sulfate-insoluble proteins measured by size exclusion chromatography; a method for predicting bread-making ability and bread quality that can quickly and easily predict the degree of bread-making ability and bread quality from yudan dough; and a method for using yudan dough to produce bread. [Solution] A method for producing bread includes the steps of: (a) preparing a Tangzhong dough by mixing wheat flour with warm water, or by mixing wheat flour with water and then heating; (b) preparing a sodium lauryl sulfate-soluble protein sample and a sodium lauryl sulfate-insoluble protein sample using a portion of the Tangzhong dough; (c) measuring the soluble polymer protein signal area value by size exclusion chromatography; (d) measuring the insoluble polymer protein signal area value by size exclusion chromatography; and (e) preparing bread dough using the Tangzhong dough, wherein the value obtained by dividing the soluble polymer protein signal area value by the insoluble polymer protein signal area value and multiplying the result by 100 is adjusted to be greater than 44 and less than 70.

The present disclosure provides a formulation for extracting a water-insoluble protein, as well as a kit and a method using the formulation to rapidly detect gluten allergen. The formulation includes a Tris buffer and an ionic liquid.

The application belongs to the technical field of food engineering, and particularly relates to a food-grade emulsion enzyme catalysis system, a construction method and application in functional lipid synthesis. The food-grade Pickering emulsion is formed by taking water-insoluble protein-chitin nanoparticles and a lipase CRL compound as the water phase and taking a reaction substrate fatty acid as the oil phase, and is used for preparing phytosterol esters. The water-insoluble protein-chitin nanoparticles have excellent dispersibility, stability and high surface positive charge, the lipase is adsorbed on the surface of the nanoparticles through electrostatic and hydrogen bond interaction, has reduced interfacial tension and enhanced interfacial activity, the lipase in the Pickering emulsion stabilized by the nanoparticles is distributed on the oil-water interface, the catalytic efficiency of the lipase is effectively improved, and the food-grade Pickering emulsion interface catalysis is used to efficiently synthesize phytosterol esters.

The invention discloses emulsion gel with stable walnut-based compound particles and a preparation method of the emulsion gel. Degreased walnut meal is used as a raw material, and alkali treatment and acid treatment are sequentially performed to obtain an insoluble walnut multi-component compound; sequentially carrying out shearing and ultrasonic treatment on the walnut multi-component compound to obtain walnut-based compound particles; and taking the walnut-based compound particles as an interface stabilizer, and carrying out high-speed shearing to prepare the emulsion gel. According to the prepared emulsion gel, the gelation process is completed while shearing emulsification is conducted, the preparation method is simple and efficient, processing by-products such as insoluble protein, polysaccharide and polyphenol in the degreased walnut meal are fully utilized, and a new treatment mode is provided for high-value utilization of grain and oil by-products.

The invention provides a donkey-hide gelatin purification method and application thereof, and belongs to the technical field of donkey-hide gelatin. The colla corii asini purification method comprises the following steps: crushing colla corii asini slices, mixing the crushed colla corii asini slices with an extracting solution, performing ultrasonic extraction and filtration, adding a compound enzyme into filtrate for enzymolysis, performing centrifugation, filtering supernate through an ultrafiltration membrane, collecting permeate liquid, and performing drying to obtain a colla corii asini extract, the extracting solution comprises L-cysteine, acetic acid and sodium chloride. According to the extracting solution disclosed by the invention, efficient dissolution of insoluble protein in donkey-hide gelatin is realized through synergistic effects of destroying hydrogen bonds in an acidic environment, collapsing hydrophobic cross-linking by L-cysteine and enhancing dissolution stability by salt ions, and active small molecule components are obtained through enzymolysis by compound enzyme. The invention further provides application of the colla corii asini extract and the radix rehmanniae preparata extract in preparation of products for nourishing yin, enriching blood and promoting lactation. Animal experiments show that by compounding the colla corii asini extract and the radix rehmanniae preparata extract, the functions of nourishing yin and enriching blood can be remarkably improved, and lactation is promoted.

The present invention concerns a method for detecting cleavage of a protein of interest in a non-purified test sample, wherein said protein of interest comprises at least a first signature peptide present in a first domain and at least a second signature peptide present in a second domain, wherein the method comprises the steps of: heating the non-purified test sample to provide a heated test sample; separating soluble from insoluble protein in the heated test sample to produce soluble and insoluble protein fractions of said heated test sample; and determining, in the soluble and / or the insoluble protein fraction of step b), a first relative level, which is the level of the first signature peptide as compared to a control level, and a second relative level, which is the level of the second signature peptide as compared to a control level, wherein cleavage is detected by a difference between said first and second relative levels in the soluble protein fraction and / or by a difference between said first and second relative levels in the insoluble protein fraction.

The invention discloses sophorolipid as well as a preparation method and application thereof. The preparation method comprises the following steps: step 1, performing streak culture for three generations; step 2, performing shaking table fermentation to obtain a primary seed solution; step 3, performing shaking table fermentation again to obtain a secondary seed solution; 4, sterilizing at high temperature, cooling and inoculating; step 5, fermenting; step 6, after fermentation is finished, collecting all sophorolipids and centrifuging; 7, collecting a product, and removing residual thalli, insoluble protein, polysaccharide and ethanol; and step 8, washing with hot water, and performing rotary evaporation to remove impurities to obtain a sophorolipid finished product. The sophorolipid is prepared through continuous batch fermentation, the production efficiency of the sophorolipid can be greatly improved, pollution and thallus aging cannot be caused, introduction of organic reagents is avoided through a multi-link green purification process, the requirement of sustainable development is better met, and the method is particularly suitable for application of the sophorolipid in the daily chemical industry.

The invention discloses a preparation method and application of magnetic composite particles. The magnetic composite particles are prepared by pretreating silicate minerals, sequentially performing nanocrystallization treatment and lipophilic modification on a fibrous organic base material, and compounding the pretreated silicate, the modified organic base material and magnetic microspheres. According to the preparation method, the solubility and dispersity of the base material in water can be effectively improved through nanocrystallization of the organic base material, and the affinity with insoluble protein and lipid substances is improved through lipophilic modification of the organic base material; the base material is compounded with the magnetic particles, so that the magnetic recoverability is realized; the composite particles are used for recycling protein and lipid substances in aquatic product processing wastewater according to requirements.