7 results about "Insoluble protein" patented technology

The present application belongs to the technical field of biological pharmacy, and particularly relates to a method for extracting and purifying spinosad. The method for extracting and purifying spinosad from saccharopolyspora spinosa fermentation broth by combining enzymolysis and acid extraction reduces the amount of organic solvent; the membrane separation technology is used to purify the spinosad extract, the microfiltration and ultrafiltration are used to remove the bacterial bodies, insoluble proteins, colloid, pigments and other macromolecular impurities, and the nanofiltration is used to remove small molecular water-soluble impurities, so as to realize the purification of the extract; the two-phase extraction is used to realize the second purification of the spinosad; and the evaporation crystallization is used to realize the third purification of the spinosad. Compared with the prior art, the method for extracting and purifying spinosad has the advantages of simple process, high efficiency, environmental protection, low consumption, and the like.

This method for producing an insoluble protein-containing powder product involves mixing a raw material containing an insoluble protein and a lipid with water, and subsequently subjecting the mixture to a spray drying treatment, wherein the insoluble protein-containing powder product in which dispersibility of the insoluble protein with poor dispersibility is improved is produced by adjusting the ratio of the lipid to the raw material containing the insoluble protein and the lipid and / or by adjusting the outlet temperature of a spray dryer in the spray drying treatment.

The application belongs to the technical field of protein purification, and discloses a protein purification method based on condensate, which comprises the following steps: firstly, constructing a target expression vector; secondly, expressing a fusion protein with an LplA label in E. coli; thirdly, ultrasonically crushing the E. coli, centrifuging the crushing liquid, and taking supernatant; fourthly, performing first temperature change, heating the supernatant, centrifuging and collecting the fusion protein after the fusion protein forms condensate, discarding the supernatant, and taking the precipitate; and fifthly, performing second temperature change, redissolving the precipitate obtained in the fourth step in a buffer at low temperature, removing insoluble proteins by centrifugation at low temperature, and taking the supernatant as the fusion protein with the LplA label after purification. The application connects the temperature-responsive purification label LplA which can form condensate to the target protein, and only needs to perform two temperature change treatments to quickly obtain the target protein with high purity, without the need of complex chromatography equipment and expensive fillers, and the application is suitable for large-scale production and purification of functional proteins.

The invention provides a method for detecting the content of protein in tobacco. The method comprises the following steps: 1) mixing a tobacco sample to be detected with a solution of an enzyme preparation, and reacting; (2) adding a dimethyl sulfoxide aqueous solution into a product obtained in the step (1), mixing, and extracting protein; the enzyme preparation comprises pectinase, cellulase and hemicellulase.The method has the advantages that soluble and insoluble proteins in tobacco leaves can be sufficiently extracted by the aid of the method, and the method is high in selectivity and sensitivity and high in accuracy of detection results.

The application belongs to the technical field of food engineering, and particularly relates to a food-grade emulsion enzyme catalysis system, a construction method and application in functional lipid synthesis. The food-grade Pickering emulsion is formed by taking water-insoluble protein-chitin nanoparticles and a lipase CRL compound as the water phase and taking a reaction substrate fatty acid as the oil phase, and is used for preparing phytosterol esters. The water-insoluble protein-chitin nanoparticles have excellent dispersibility, stability and high surface positive charge, the lipase is adsorbed on the surface of the nanoparticles through electrostatic and hydrogen bond interaction, has reduced interfacial tension and enhanced interfacial activity, the lipase in the Pickering emulsion stabilized by the nanoparticles is distributed on the oil-water interface, the catalytic efficiency of the lipase is effectively improved, and the food-grade Pickering emulsion interface catalysis is used to efficiently synthesize phytosterol esters.

The invention discloses emulsion gel with stable walnut-based compound particles and a preparation method of the emulsion gel. Degreased walnut meal is used as a raw material, and alkali treatment and acid treatment are sequentially performed to obtain an insoluble walnut multi-component compound; sequentially carrying out shearing and ultrasonic treatment on the walnut multi-component compound to obtain walnut-based compound particles; and taking the walnut-based compound particles as an interface stabilizer, and carrying out high-speed shearing to prepare the emulsion gel. According to the prepared emulsion gel, the gelation process is completed while shearing emulsification is conducted, the preparation method is simple and efficient, processing by-products such as insoluble protein, polysaccharide and polyphenol in the degreased walnut meal are fully utilized, and a new treatment mode is provided for high-value utilization of grain and oil by-products.

The invention discloses sophorolipid as well as a preparation method and application thereof. The preparation method comprises the following steps: step 1, performing streak culture for three generations; step 2, performing shaking table fermentation to obtain a primary seed solution; step 3, performing shaking table fermentation again to obtain a secondary seed solution; 4, sterilizing at high temperature, cooling and inoculating; step 5, fermenting; step 6, after fermentation is finished, collecting all sophorolipids and centrifuging; 7, collecting a product, and removing residual thalli, insoluble protein, polysaccharide and ethanol; and step 8, washing with hot water, and performing rotary evaporation to remove impurities to obtain a sophorolipid finished product. The sophorolipid is prepared through continuous batch fermentation, the production efficiency of the sophorolipid can be greatly improved, pollution and thallus aging cannot be caused, introduction of organic reagents is avoided through a multi-link green purification process, the requirement of sustainable development is better met, and the method is particularly suitable for application of the sophorolipid in the daily chemical industry.