96 results about "Insoluble protein" patented technology

The present disclosure relates to an aqueous process for the preparation of a protein isolate and a hydrolyzed protein concentrate from an oilseed meal, optionally comprising:

• • mixing an oilseed meal with an aqueous solvent to form a slurry;

optionally treating the slurry with phytase y;

separating the slurry with a solid/liquid separation to form:

• a liquid phase, comprising the aqueous solvent, soluble protein and oil; and
• a solid phase comprising insoluble protein;

separating the liquid phase to form:

• an oil phase; and
• an aqueous protein phase;

subjecting the aqueous protein phase to membrane filtration to obtain a protein solution; and drying the protein solution to obtain the protein isolate

subjecting the insoluble protein to enzymatic hydrolysis, and

subjecting the hydrolyzed protein to membrane filtration to obtain an amino acid and peptide solution; and drying the amino acid and peptide solution to obtain the hydrolyzed protein concentrate.

Whole grain, such as wheat, barley, rye, and/or rice, can be processed by (a) steeping the grain in an aqueous liquid to produce softened grain, (b) milling the softened grain to produce milled grain, (c) liquefying the milled grain by contacting it with amylase and heating it to a temperature of at least about 50° C., producing a liquefied material, (d) at least partially saccharifying the liquefied material by contacting it with amyloglucosidase at a temperature of at least about 50° C., producing a first saccharified material, and (e) separating fiber and germ from the first saccharified material, producing a screened material that is substantially free of fiber and germ. The process also includes the steps of (f) further saccharifying and fermenting the screened material with a microorganism that produces ethanol, thereby producing a broth that comprises ethanol, soluble protein, and insoluble protein, and (g) separating ethanol from the broth. A protein-rich product can be recovered from the broth that comprises both gluten from the grain and microorganism from the fermenting step.

The invention discloses a method for extracting sialic acid from bird nests by means of crystallization. The method comprises the steps of soaking the bird nests with deionized water and removing impurities; then pulping and crushing, heating under a high pressure, centrifuging, drying, dissolving and crystallizing to obtain the sialic acid. According to the method disclosed by the invention, hydrolysis of the glycoproteins of the bird nests is accelerated through serious heating by means of high-pressure heating, and the combined-state sialic acid is converted to a free-state sialic acid; the high-pressure heating avoids the introduction of other ingredients, thereby simplifying the subsequent separation and purification steps; the sialic acid crude product obtained by freeze-drying is prepared into alcohol-saturated solution by virtue of the property that the sialic acid is slightly dissolved in alcohol but proteins and polysaccharides are insoluble in alcohol, and therefore on one hand, the impurities such as alcohol-insoluble proteins and polysaccharides are removed, on the other hand, the solvent polarity is changed by adding a non-polar solvent, and sialic acid crystals are obtained according to the different seperation sequences of the sialic acid and the impurities. According to the method disclosed by the invention, sialic acid extraction steps are simplified, and industrial pollution is reduced.

The present invention is concentrated soybean protein modifying method and features that water insoluble protein obtained through low temperature separation of defatted soybean residue is produced into concentrated protein powder in high water solubility through water dilution, pH regulation, heating, homogenization, drying and other steps. The concentrated protein powder has water solubility as high as 70-80 %, protein content of 67-70 %, processing performance of being emulsified, thickened, gelatinized, etc. and expanded use range. In addition, the process of the present invention has low cost, less pollution and good product taste.

The invention discloses a preparation method of a squid skin protein active peptide, which comprises the following steps: cleaning squid skin, cutting into blocks, adding NaOH water solution, soaking for 2-8 days for decolorization and further washing with water till the pH is neutral; adding CaOH2 solution, soaking for 2-4 days for color fixation, further washing with the water till the pH is neutral; processing for 0.5-1.5 hours in the water with the neutral pH at the temperature of 55-75 DEG C for filtration, removing insoluble proteins and extracting for getting squid skin protein solution; and finally performing high-pressure processing on the solution for 30-120 minutes at the temperature of 110-121 DEG C for getting the squid skin protein active peptide. The squid skin is processed by the dual-alkali process, the decolorization is firstly performed and then the color fixation is performed; the high-pressure processing method is used for processing squid skin proteins for preparing the biological active peptide, the preparation process is simple, the cost is low and the applicability is strong; and the prepared active peptide has the effects of oxidation prevention, senility resistance, liver protection, enhancement of immunity and the like.

The invention discloses a high-protein tea lozenge, which consists of the following components in percentage by mass: 18 to 90 percent of tea powder prepared by an enzyme processing process, 8.9 to 80 percent of protein powder or milk powder and 1 to 10 percent of walnut powder. Compared with the prior art, the high-protein tea lozenge has the advantages that: (1) the content of soluble protein in tea prepared by the conventional process is low, the protein powder or the milk powder is added into ingredients and the walnut powder is mixed in order to enhance the nutritive value of the tea lozenge, and thus, the tea lozenge is nutritional health-care food; (2) by enzyme processing, cellulose, insoluble protein, ester catechin and the like in the tea are hydrolyzed, so that the content of soluble ingredients is increased; and (3) due to the hydrolysis of the ester catechin, the bitter and astringent taste of the tea is easy to reduce, and due to the addition of peroxidase and catalase, the content of theaflavin and thearubigins is increased, the drinking value and health-care value of black tea are improved, and the taste of the black tea is improved.

Mouthfeel can be improved by processing a beverage product having an insoluble food component with a static mixer. The beverage product can be a soy beverage containing insoluble protein components. The static mixer serves to reduce the average particulate size of the insoluble food component.

This invention relates generally to a method for purifying Interleukin-4 or related variants from an Escherichia coli culture medium, where Interleukin-4 and its derivatives are expressed as insoluble protein aggregates, so-called inclusion bodies.

The present invention provides use of a protein conjugate comprising a “ble” protein, which has specific binding properties. The protein conjugates are capable of binding reversibly to an antibiotic from the bleomycin family, which property is exploited in a variety of immobilisation methods. In preferred aspects of the invention, the conjugates are used as markers for protein expression and/or folding, or for affinity tagging. The present invention also provides a probe comprising an array of an immobilised antibody from the bleomycin family, which acts as an analyte capture moiety. In another aspect, a purification media is provided, which comprises an antibiotic of the bleomycin family as an analyte capture moiety. Also provided is a method for generating soluble forms of an insoluble protein by expressing the protein as a “ble” fusion protein and selecting in the presence of an antibiotic from the bleomycin family. In a further aspect, the “ble” protein is expressed as a fusion protein in a cell into which is introduced a labelled antibiotic of the bleomycin family, thereby allowing identification of the cellular localisation of the protein.

The invention is directed to a method of determining whether a non-purified sample contains a target protein bound to a ligand of interest comprising the steps of: a) exposing said non-purified sample to a temperature which is capable of causing or enhancing precipitation of the unbound target protein to a greater extent than it is capable of causing or enhancing precipitation of the target protein bound to said ligand; b) processing the product of step a) in order to separate soluble from insoluble protein; and c) analysing either or both the soluble and insoluble protein fractions of step b) for the presence of target protein, wherein said target protein is not detected on the basis of enzymatic activity of a tag, peptide, polypeptide or protein fused thereto. Particularly, the invention may be used to determine whether drugs can bind to their protein targets in samples derived from patients to ascertain whether a certain drug can be used in a therapy for that patient. Additionally, the invention is directed to an instrument for use in the methods of the invention and use of a kit in the methods of the invention comprising an antibody and/or a non-protein fusion tag.

The invention discloses a preparation method of an egg white protein-catechin free radical graft and belongs to the field of food technology. The method comprises the following steps: diluting by adding 9 times volume of water into egg white until the mass fraction of the protein is 1%, adjusting pH to about 5.0, stirring for 10-20 min, naturally precipitating for 1 h, filtering to remove insoluble proteins, taking 50 mL of an egg white solution into a 100mL triangular flask, adding 0.5-2.0 mL of 5M hydrogen peroxide and 0.0625-0.5 g of ascorbic acid, uniformly stirring and placing at room temperature for 2h, adding 0.025-0.1 g of catechin, reacting at 25 DEG C for 12-48 h, dialyzing the sample at 4 DEG C for 12-60 h, changing water every 6 h to ensure that unreacted free polyphenol is completely dialyzed out, and finally freeze-drying to obtain the sample. According to the egg white protein-catechin free radical graft obtained by the above method, the highest DPPH free radical scavenging rate is 65.29%, the ABTS free radical scavenging rate is 94.17%, and the highest reducing power reaches 0.649 Abs.

The invention relates to a preparation method of corn starch. The method comprises: smashing immersed niblets so as to separate germs, subjecting degermed corn-bran and endosperm-containing niblet tissues to grinding so as to separate fiber, separating the obtained crude starch milk so as to obtain gluten water and starch milk, and washing the starch milk. Specifically, the gluten water is then concentrated to obtain a concentrate and process water containing insoluble protein. The process water is then mixed with a flocculating agent solution for floatation separation. The flocculating agent involved is a material able to make insoluble protein in the process water flocculate and/or subside. The method of the invention can make the insoluble protein and water in the process water effectively separated, thus substantially enhancing the yield of gluten protein powder. In addition, the protein flocculated process water can be reused to immerse corn and wash germs and fiber cyclically, basically can reach the immersion and washing effects of fresh water, and substantially improves the quality of refined starch milk.

The invention provides a method for extracting iodine from kelp. The method comprises the following steps of kelp immersion, kelp immersion liquid purification, acidification oxidation conversion into dissociative iodine, adsorption by a resin, reduction desorption and iodine oxidation precipitation. Concretely, the method comprises the following steps of preparing an immersion liquid of kelp as a raw material, carrying out multistage reaction purification separation, adding calcium oxide or calcium hydrate into the immersion liquid subjected to purification separation for air floatation to remove water-soluble alginate and proteins which can react with calcium ions, adding an alkali into the immersion liquid treated by the previous step to remove alkaline insoluble substances such as fucoidin and water-insoluble impurities by air floatation separation under strong alkaline conditions, and adding an acid into the immersion liquid treated by the previous step to further remove acid-insoluble proteins by air floatation under acidic conditions. Through removal of the organic impurities, competitive adsorption of elemental iodine in resin adsorption is avoided and resin adsorption effects and an iodine yield are improved. Compared with the method for treating an immersion liquid only by a caustic soda solution, the method provided by the invention can effectively purify a kelp immersion liquid and improve an iodine yield.

A preparation method of corn starch comprises the following steps: soaking corn grains, crushing the soaked corn grains, separating germs, grinding the degermed corn grains with corn bran and endosperm, separating fibers, separating the obtained crude starch milk to obtain gluten water and starch milk, and washing the starch milk, wherein the treatment method of the gluten water comprises gluten water concentration so as to obtain a concentrate, and process water comprising insoluble protein, mixing the process water with a flocculating agent, performing solid-liquid separation; the flocculating agent is a substance which can flocculate and/or precipitate insoluble protein in the process water. The method of the invention can perform effective separation of the insoluble protein in the process water from water, and thus greatly increase the yield of gluten protein powder. In addition, the process water after protein flocculation is recycled for the soaking of corn and the washing of germs and fibers, and basically reaches the effect for soaking and washing with fresh water, and thus the quality of fine starch milk is significantly improved.

The invention provides a method for efficiently extracting sheep viscus tissue protein, i.e. a frozen crushing homogenate method and a vortex mixing method are adopted, the protein quantification is carried out on the extracted viscus tissue protein of livers, spleens, lungs, kidneys and the like of the sheep, the extracted protein is separated through polyacrylamide gel electrophoresis, and the expression of the transgenosis sheep green fluorescent protein (GFP) genes is detected by western blot. The method has the technical characteristics that: 1, the concentration of the extracted protein is high, and the cost is low; 2, the method is applicable to the extraction of viscus tissue protein of adult or young sheep; and 3, insoluble protein is little, the abandoned tissue residue is little, and important practical values are realized.

The invention discloses a making method of insect flavor beautifying tea. The method comprises the following steps: sun withering, rocking of green leaves, fermentation, enzyme deactivating, rolling, packed rolling, ball breaking, drying to obtain primary tea, careful choosing of the primary tea, and baking to obtain the finished insect flavor beautifying tea. Protease is added through a reasonable technology to decompose insoluble proteins in the tea leaves in order to make the insoluble proteins become free amino acids, so the content of amino acids, especially amino acids having a fresh and sweet taste, in oolong tea concentrate is increased, the fresh taste of the tea leaves is induced, and the integral taste of a tea soup is improved. The alcoholic fermentation degree is 75-85%, so water has a sweet taste and a unique flavor. In the production process, no pesticides can be used, fresh leaves must be bitten and eaten by small green leaf cicadas, and insect saliva and tea enzymes are mixed to obtain a unique flavor. Bai Hau Oolong tea leaves and flowers undergo mixing fermentation, so the nutrients of two teas are kept, and the flavors of the two teas are mixed to realize a rich flavor. The insect flavor beautifying tea has an alcohol fruit flavor, a honey flavor and a various flowers-integrated unique original flavor.

The invention relates to a clone and expression plasmid vector. The clone and expression plasmid vector contains a green fluorescent protein (GFP) gene segment which contains a tobacco etch virus (TEV) enzyme recognition site and is used for encoding the gene sequence of NusA protein, and of which two sides are provided with bovine serum albumin (BsaI) restriction enzyme cutting sites. The constructed vector can realize the quick and efficient clone and protein expression of the targeted gene without depending on ligase. The NusA protein label and the TEV enzyme recognition site are introduced into the vector; and by the NusA protein label, the solubility of the insoluble protein can be improved, and the protein can be separated and purified conveniently; and a TEV enzyme recognition sequence is positioned at the downstream of the gene sequence of the NusA protein label, the purified protein is cut by TEV enzyme, and the NusA protein label can be cut off, so that the natural targeted protein can be obtained. The TEV enzyme is the enzyme which is the most economic and applicable at present, so that the experimental cost is greatly saved.

The invention relates to a separation and purification method for phytine, which specially comprises the following steps: (1) sedimentation processing; (2) resin adsorption; (3) desorption elution; (4) neutralization and precipitation; and (5) sheet frame filtering. According to the invention, the insoluble protein content of corn water is greatly reduced, the problem the hybridization column is blocked by corn water is completely solved, the protein content of phytine is reduced, and the quality and yield of phytine are further improved. And through the adoption of a novel adsorption resin, the yield of products is significantly improved.

The invention provides a sewage treatment method of a casing plant, specifically relates to a water pollution treatment method for industrial production of heparin sodium in the casing plant, and aims at solving the problems of high concentration of CODcr (Chemical Oxygen Demand), BOD5 (Biochemical Oxygen Demand), Na<+>, Cl<-> and ammonia nitrogen in sewage of the casing plant, existence of macromolecular organic matter, and large change in effluent volume. The method comprises the following steps: stopping dross through a grid; separating suspended solids; removing dust; separating oil; separating animal fat and insoluble protein on the surface layer; regulating water volume and quality; treating by decomposing by anaerobion through an UASB (Upflow Anaerobic Sludge Blanket) anaerobic reactor; treating by decomposing by aerobic bacteria through a two-stage biological contact oxidation pond; treating sludge; separating by air flotation; draining. The sewage treatment method of the casing plant is applicable to the field of treatment of sewage produced by the casing plant.

The invention discloses a method for continuously extracting L-tryptophan from a fermentation liquor. The method comprises the following steps: step 1, regulating the pH value of the fermentation liquor to the range from 3.8 to 4.5 by using hydrochloric acid in a fermentation tank, and heating for inactivation to form a feed liquor; step 2, feeding the feed liquor into a high-speed tube centrifuge, and removing bacteria and insoluble protein separated out to form a centrifugal effluent; step 3, removing most pigment from the centrifugal effluent by using a membrane of 3000 Daltons, and feeding the decolored effluent into a tubular nanofiltration membrane system of 200 Daltons for nanofiltration until the feed liquor is slightly turbid, and then forming a concentrated liquor; step 4, quickly cooling and crystallizing the concentrated liquor through a tubular crystallizer, and subjecting the crystallized liquor to solid-liquid separation to obtain crude tryptophan; step 5, dissolving the crude tryptophan in hot pure water, and adding the hydrochloric acid to regulate the pH value to the range from 4.0 to 5.0 for helping solubilization; decoloring by using activated carbon, and then treating the decolored liquor by the circulating methods from the step 2 to the step 4 in the same way, and finally, drying to obtain white refined tryptophan. The method provided by the invention has the advantage that the quality and yield of the product are improved.

The invention discloses a high-quality ornithine extraction method. The method comprises 1, microfiltration: placing a ornithine fermentation broth in a primary filtration device to obtain a microfiltration dialysis liquid and filtering the microfiltration dialysis liquid through a ceramic membrane to remove impurities such as bacteria, colonies, mycelium, insoluble proteins and soluble macromolecular proteins, 2, decoloring treatment, 3, concentration treatment, 4, pH adjustment, 5, concentration crystallization, 6, water washing, 7, drying treatment, and 8, crystallization and crushing. The high-quality ornithine extraction method can be used for a long time in a safe and high-efficiency manner, is easy to maintain, has simple production processes and reduces a production cost.