Method for preparing stable soybean protein-sterol granules through combining heat treatment with high-pressure micro jet treatment

A high-pressure micro-fluidics and soybean protein technology, applied in the field of deep processing of grain by-products, can solve the problems of poor protein thermal stability, reduce food shelf life, flocculation, etc., achieve good resolubility, improve shelf life, and simple production process.

Active Publication Date: 2018-01-12
GUANGXI WUZHOU BINGQUAN IND SHAREHLDG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most proteins have poor thermal stability, and after high temperature treatment at 120°C, severe flocculation, aggregation and other instability phenomena will occur, which greatly reduces the shelf life of food

Method used

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  • Method for preparing stable soybean protein-sterol granules through combining heat treatment with high-pressure micro jet treatment
  • Method for preparing stable soybean protein-sterol granules through combining heat treatment with high-pressure micro jet treatment
  • Method for preparing stable soybean protein-sterol granules through combining heat treatment with high-pressure micro jet treatment

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Experimental program
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Effect test

Embodiment 1

[0054] A method for preparing functional soybean protein-sterol particles by heat treatment combined with high-pressure micro-fluidic treatment, the steps are as follows:

[0055] (1) Disperse soybean protein in deionized water at a material-to-liquid ratio of 0.8:10 (w / v, g / mL), adjust the pH to 7.0, stir at room temperature 15-30°C for 2 hours, and heat in a water bath at 90°C for 30 minutes. Rapidly cool down to room temperature, and pass high-pressure micro-jet once under a pressure of 100Mpa to obtain the desired protein solution;

[0056] (2) Disperse the dry phytosterol powder in absolute ethanol at 0.02:1 (w / v, g / mL), and stir in a water bath to obtain the required sterol-ethanol solution;

[0057] (3) Add the sterol-ethanol solution to the casein solution at a volume ratio of 0.5:10 under the condition of a high-speed shearing machine at a rate of 13000rpm, and perform ultrasonic treatment after mixing for 4 minutes (power 240W, time 10min, interval 50%) ), then the ...

Embodiment 2

[0065] A method for preparing functional soybean protein-sterol particles by heat treatment combined with high-pressure micro-fluidic treatment, the steps are as follows:

[0066] (1) Disperse soybean protein in deionized water at a material-to-liquid ratio of 1:10 (w / v, g / mL), adjust the pH to 7.0, stir at room temperature 15-30°C for 2 hours, and place in an autoclaved pot at 120°C Heating for 20 minutes, cooling down to room temperature rapidly, and passing high-pressure micro-jet once under a pressure of 100Mpa to obtain the desired soybean protein solution;

[0067] (2) Disperse the dry phytosterol powder in absolute ethanol at 0.02:1 (w / v, g / mL), and stir in a water bath to obtain the required sterol-ethanol solution;

[0068] (3) Add the sterol-ethanol solution to the casein solution at a volume ratio of 0.5:10 under the condition of a high-speed shearing machine at a rate of 13000rpm, and perform ultrasonic treatment after mixing for 4 minutes (power 240W, time 10min, ...

Embodiment 3

[0075] A method for preparing functional soybean protein-sterol particles by heat treatment combined with high-pressure micro-fluidic treatment, the steps are as follows:

[0076] (1) Disperse soybean protein in deionized water at a material-to-liquid ratio of 1:10 (w / v, g / mL), adjust the pH to 7.0, stir at room temperature 15-30°C for 2 hours, and heat in a water bath at 120°C for 20 minutes. Rapidly cool to room temperature, and pass high-pressure micro-jet once at a pressure of 120Mpa to obtain the desired soybean protein solution;

[0077] (2) Disperse the dry phytosterol powder in absolute ethanol at 0.02:1 (w / v, g / mL), and stir in a water bath to obtain the required sterol-ethanol solution;

[0078] (3) Add the sterol-ethanol solution to the casein solution at a volume ratio of 0.5:10 under the condition of a high-speed shearing machine at a rate of 13000rpm, and perform ultrasonic treatment after mixing for 4 minutes (power 240W, time 10min, interval 50%) ), then the m...

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Abstract

The invention discloses a method for preparing stable soybean protein-sterol granules through combining heat treatment with high-pressure micro jet treatment. The method comprises the following stepsof dispersing soybean protein in deionized water, regulating pH of dispersion liquid, performing centrifugation, performing heat treatment on the dispersion liquid and performing high-pressure micro jet treatment so as to obtain a protein solution; under the high-speed shearing condition, adding a sterol-ethanol solution to the protein solution, and performing uniform mixing; and performing ultrasonic homogenizing on the mixed liquid of protein and sterol, performing evaporation to remove ethanol, performing centrifugation to remove unembedded sterol and insoluble protein, and performing freeze drying so as to obtain soybean protein-sterol nanometer granule dry powder. The invention develops a novel protein modification technique, a carrier for conveying sterol, being small in granule diameter and high in stability, is produced, the method is simple and convenient to operate, continuous production can be performed, and the method can be widely applied to production of functional sterolbean milk.

Description

technical field [0001] The invention relates to sterols, in particular to a method for preparing stable soybean protein-sterol granules by combining heat treatment with high-pressure micro-jet treatment, and belongs to the technical field of deep processing of grain by-products. Background technique [0002] Sterols are biologically active ingredients widely present in plant cell membranes. They have excellent physiological effects such as lowering cholesterol, anti-inflammatory and antipyretic, anti-cancer, antagonizing cholesterol, preventing cardiovascular diseases, promoting animal growth and improving human body health. key to life". As early as 1999, phytosterols have been approved by the US FDA to be added to food, and the food can use the "healthy" label. At the same time, a large number of research reports indicate that daily intake of more than 1.39g of phytosterol esters or more than 3.49g of phytostanol esters can effectively lower blood cholesterol. Based on t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/17A23L33/10
Inventor 王金梅王梦萍韦翠兰杨晓泉齐军茹
Owner GUANGXI WUZHOU BINGQUAN IND SHAREHLDG
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