164results about How to "Low in protein" patented technology

The invention provides a production method of human fibrinogen, which is against the method that domestic conventional manufacturers extract and separate human fibrinogen from component I, and the method for extracting fibrinogen from cryoprecipitate is adopted; an S / D method and a water-bath heat treating method are adopted to carry out double-virus inactivation, thereby effectively inactivating fat enveloped virus and non-fat enveloped virus, and ensuring the security of products; the purity of extracted products reaches as high as more than 90%, the protein content of impurities is low, and the clinical side reaction of the product is small. In the production process of the human fibrinogen of the invention, glycine is utilized as a stabilizer, the freeze-drying time is prolonged about 6-8 days to ensure the temperature variation of the product to be stable in freeze-dying process, but the freeze-drying time of general manufacturers is about 3 days; after being freeze-dried for 6-8 days, the product has uniform structure, then water bath heat treatment is carried out on the product, thus the product can be evenly heated in the shortest time, thereby achieving the purposes of effectively inactivating DNA and RNA non-fat enveloped virus and simultaneously ensuring the stability of human fibrinogen.

A non-serum culture medium for culturing CHO cells is prepared from DMEM / F12 as basic culture medium, transferrin, insulin, ethanolamine, etc.

The invention provides a processing method of refined rice suitable for patients suffering from kidney disease. The processing method comprises the following steps: (I) carrying out pretreatment, specifically, soaking the cereal raw material, separating out soaking liquid, crushing and levigating until the particle size is 10-100 meshes; (II) carrying out protease hydrolysis deproteinization; and (III) carrying out dephosphorization: dephosphorizing the cereal raw material subjected to deproteinization in the step (II) by phytase hydrolysis, or addition of yeast for fermentation, or a composite mode of phytase hydrolysis and yeast fermentation, or a composite mode of yeast and lactic acid bacteria microbial fermentation or phytase hydrolysis, yeast and lactic acid bacteria microbial fermentation; separating, cleaning, separating again, drying and crushing; and (IV) performing sieving, mixing, hardening and tempering, preheating, gelatinization, extrusion forming and drying processing on the cereal raw material processed by the step (III) to obtain the refined rice. According to the method provided by the invention, the processes of deproteinization and dephosphorization of the cereal raw material can be finished at a time, the deproteinization rate and the dephosphorization rate are high, and food raw material and products are provided for the patients suffering from kidney disease and other diseases.

The invention discloses a high-sugar and low-protein tilapia feed. Based on the total mass fraction of 100%, it consists of the following components: fish meal: 0-3%, soybean meal: 16-20%, rapeseed meal: 18-21%, cotton meal: 12-16%, secondary meal: 39- 42%, soybean oil: 2-3%, choline chloride: 0.2-0.5%, sodium chloride: 0.1-0.5%, calcium dihydrogen phosphate: 1-2%, vitamin B1: 0.004-0.006%, vitamin B6: 0.002-0.004%, zinc sulfate: 0.02-0.04%, chromium chloride: 0.0001-0.0003%, vitamin premix: 0.1-0.2% and mineral premix: 0.5-1%. The invention maximizes the amount of secondary powder in the feed, adds several plant protein sources to overcome the problem of amino acid imbalance of a single protein source, and at the same time significantly reduces the amount of fish meal. By optimizing and adjusting the feed formula, supplementing suitable vitamin and mineral premixes to adjust the sugar utilization ability of tilapia, so that the tilapia can make good use of the sugar compound of the present invention, avoiding the damage of fish liver caused by high sugar, Fatty liver is formed, and even the fish body becomes obese, and the symptoms of "big belly" appear.

The invention relates to a heparin sodium purification technology. The crude heparin sodium purification technology sequentially comprises the following steps: 1, dissolving crude heparin sodium in a sodium chloride solution, and adding an alkaline protease for enzymatic hydrolysis; 2, inactivating, adding diatomite, carrying out high speed centrifugation, and removing insoluble impurities; 3, adding polysilicate, carrying out high speed centrifugation, and removing insoluble impurities; 4, adding alcohol for precipitating, and removing supernatant alcohol; 5, dissolving in the sodium chloride solution, adding hydrogen peroxide for oxidizing, carrying out high speed centrifugation after oxidation, and removing insoluble impurities; 6, adding alcohol for precipitating, and removing supernatant alcohol; and 7, filtering, and carrying out vacuum lyophilizing to obtain refined heparin sodium. The crude heparin sodium purification technology has the advantages of low content of proteins in the refined heparin sodium, and high yield of the refined heparin sodium.

The present invention relates to ''fish soluble protein powder and preparation method'', which belongs to the field of animal feed processing. A fish soluble protein powder preparation method, the fish soluble can be processed by being eliminated from fish oil and mucopolysaccharide, and then being concentrated and dried. Through eliminating from fish oil and mucopolysaccharide, the drying performance of fish soluble has greatly improved, after concentrated, the fish soluble is fed into a spray tower with tower wall cooling system, revolving sweep tower system and air humidity elimination system, the fish soluble powder is easily dried, free of moisture and hygroscope, easy preservation and transportation, at the same time the protein content in the fish soluble protein powder is not decreased, which is the lower the high quality materials for animal feed.

The invention discloses special fodder for the goose fattening stage. The special fodder comprises, by weight, 10-20 parts of corn, 20-30 parts of wheat, 15-30 parts of rice bran, 0.5-2 parts of lard oil, 2-5 parts of sweet potato leaves, 1-2 parts of grape seed oil, 10-30 parts of corn protein powder, 1-2 parts of fish meal, 1-3 parts of soybean protein concentrate, 5-10 parts of chrysanthemum dregs, 5-15 parts of dried blood, 2-6 parts of fermentation biological protein, 0.5-1.5 parts of feather meal, 2-5 parts of cotton dregs, 5-15 parts of maize germ pulp, 0.5-2 parts of rock flour, 1-5 parts of se-enriched yeast, 2-6 parts of Chinese herbal medicine additives, 0.2-0.8 part of compound enzyme, 0.2-0.8 part of phytase, 0.01-0.04 part of white ginseng, 0.1-0.5 part of armillaria mellea, 0.01-0.05 part of rhizopus oryzae, 0.02-0.08 part of lysine and 0.02-0.09 part of methionine. The special fodder is green, healthy, balanced in nutrition, remarkable in weight put-on effect and low in cost.

The invention relates to a joint preparation method of polysaccharide and protein peptide. The joint preparation method comprises the following steps: mincing abalone viscus wet materials, mixing and cooking with water; performing primary enzymatic hydrolysis by using alkaline protease; performing membrane separation by using the obtained enzymatic hydrolysate; performing secondary enzymatic hydrolysis on obtained trapped fluid by using flavorzyme; performing membrane separation; performing freeze-drying, spray drying or alcohol precipitation and vacuum drying on the obtained trapped fluid to obtain the abalone viscus polysaccharide; merging the trapped fluid with permeate liquid obtained by the primary enzymatic hydrolysis; performing freeze-drying or spray drying after reverse osmosis or vacuum concentration processing to obtain the abalone viscus protein peptide. The joint preparation method has the advantages that the operation is simple and practical, both the polysaccharide and the protein peptide can be simultaneously prepared, and the production efficiency is high.

A provided preparation method for wool keratin regenerated cellulose fiber comprises the concrete steps: firstly preparing a viscose-rayon spinning solution, then preparing a wool keratin solution, then mixing the viscose-rayon spinning solution and the wool keratin solution, then adding a crosslinking agent, mixing and performing wet-process spinning, so as to obtain wool keratin regenerated cellulose fiber. The wool keratin regenerated cellulose fiber prepared by employing the method has the protein content of 6% or more, and the production technology is simple in process and high in production efficiency.

The invention relates to a serum-free culture medium which comprises a basal culture medium, insulin, iron-containing food additive, sodium arsenite, fibronectin, glucagon, hepatocyte growth factor and myllicin. In addition, the invention further discloses a stem cell culture method. The stem cell culture method includes the following steps: acquiring stem cells; adopting the serum-free culture medium for in vitro culture. By the serum-free culture medium, the problem of difficulty in cell purifying due to pollution of pathogens carried by a serum culture medium can be avoided, stability of cell production can be improved, cell proliferation amount can be increased, and cell activity can be enhanced.

The invention discloses a method for continuously cropping and ecologically breeding macrobrachium nipponensis and hydrilla verticillata. The method is used for breeding the macrobrachium nipponensis by means of continuously cropping the macrobrachium nipponensis and the hydrilla verticillata. The method has the advantages that eutrophic substances and harmful substances such as residual feed and feces in ponds can be converted by the hydrilla verticillata owing to a technical mode for ecologically and continuously cropping the macrobrachium nipponensis and the hydrilla verticillata, and a characteristic of strongly inhibiting generation of some algae of the hydrilla verticillata is sufficiently utilized, so that purposes of purifying breeding water and ecologically breeding the macrobrachium nipponensis can be achieved; breeding byproducts such as ammonia nitrogen and nitrite in bottom sediments of the ponds can be consumed by means of planting the hydrilla verticillata, effects of purifying the bottom sediments and maintaining excellent ecological environments of the ponds can be realized, the macrobrachium nipponensis is in the suitable ecological environments of the ponds in total breeding procedures, suitable habitat environments and palatable green feed are provided for growth of the macrobrachium nipponensis by the hydrilla verticillata, the bred macrobrachium nipponensis is high in yield and good in quality, and the price of the macrobrachium nipponensis can be increased; spores and grass seeds of the hydrilla verticillata can be offered for sale, so that an extra economical benefit can be created.

The invention discloses a middle broiler feed. The middle broiler feed comprises the following raw materials in parts by weight: 60-80 parts of corns, 5-30 parts of soybean meal, 1-10 parts of corn gluten meal, 0-5 parts of fish meal, 0.5-3 parts of soybean oil, 0.8-2.5 parts of fine stone powder, 0.8-2.5 parts of calcium hydrophosphate, 0.1-1.2 parts of amino acid, 0.1-0.8 part of vitamin premix and 0.3-1.2 parts of microelement premix. The amino acid level of the middle broiler feed adapts to that of an animal according to an ideal amino acid mode; the digestibility is improved, and particularly, the utilization rate of feed proteins and the utilization rate of amino acid are improved; generation of ammonia gas in a henhouse is reduced; synthetic amino acid is appropriately added, and the crude protein level is correspondingly reduced, so that protein feed resources can be saved, and the output of nitrogen in the excreta of livestock and poultry can be reduced; the daily gain is high, the feed conversion ratio is low, the raising cycle is greatly shortened, the slaughtering rate is improved, and economic benefits are remarkable.

The present invention provides a serum free medium used for cultivating CHO cellular extensively, and discloses the prescription of the culture medium. DMEM / F12 is used as a base culture medium and is added with the components such as vitamin C, transferring, neovaricaine, hydroxyl sodium butyrate and so on. The present invention has the advantages that no animal blood serum is contained and the content of albumen is low; the cultivating effect of the CHO cellular is good, and the CHO cellular can obtain the same effect with the culture medium containing blood serum.

The invention belongs to the field of food, and provides a vegetable meat sausage and a preparation method thereof. The vegetable meat sausage comprises the following raw materials by weight: 15-40% of vegetable protein, 10-20% of vegetable oil, 1-5% of water-soluble colloid, 1-10% of starch, 0.2-5% of an emulsifier, 0.1-1% of a flour product improver, 1-10% of seasonings and essence and the balance of ice water. The vegetable meat sausage is prepared by adding seasonings and non-meat-source essence and performing chopping, stirring, filling or high-temperature sterilization and packaging processes. The process formula of the prepared vegetable meat sausage can be used for preparing sausages, vegetable meat hams or vegetable meat products with other shapes or filling modes. The vegetable meat sausage has the unique elastic and crisp feeling and shredded meat feeling of the chopped meat sausage, has the protein content up to 20% or above, and is a high-protein vegetarian product.

The present invention is the technological process of extracting gibberellic acid GA4+7 from gibberellic acid fermenting liquid. The technological process includes the following steps: regulating the pH value of the filtrate of fermenting liquid to 4.5-4.8 before further filtering through available filter cake to eliminate protein, heavy metal and metal ion; regulating pH value to 6.5-6.8 before concentrating the filtrate by 18-20 times; regulating pH value to 4.2 before extracting with ethyl acetate; centrifuging in a high speed centrifuge, concentrating, diluting with n-butanol, precipitating in N.N-methyl benzylamine, and separating alcohol phase and water phase to obtain GA4+7 of 90 % over purity and GA4 / GA7 ratio of 2 to 1. The present invention has high GA4+7 yield and low production cost.

The invention provides a method for extracting sodium hyaluronate from a fermentation liquor containing hyaluronic acid, which comprises the steps of fading and degerming the diluted fermentation liquor which contains hyaluronic acid, filtering, adding CTAB into the filtrate, centrifugally separating, adding sodium chloride solution into the sediment and dissociating, pouring the dissociated solution onto an anion exchange column DEAE-52 chromatographic column, depositing the solution passed through the column with ethanol, and centrifugally drying so as to obtain the powder sodium hyaluronate solid. The sodium hyaluronate extracted by using the method of the invention has the advantages of high yield, high product purity, low protein content, simple process and convenient operation.

The invention belongs to the field of biological fermentation, and particularly relates to a galactomyces candidum and a method for treating high-ammonia-nitrogen biogas slurry by using the galactomyces candidum to produce single cell protein. The invention has the following specific technical scheme: the galactomyces candidum has a preservation number of CGMCC No.18570. The method of fermenting the biogas slurry comprises the following steps: taking the biogas slurry, performing filtration to remove large-particle impurities, adding a carbon source, adjusting a carbon-to-nitrogen ratio to 6:1to 10:1, and adjusting the pH to 5.5-7 to obtain a fermentation substrate; inoculating the fermentation substrate with the galactomyces candidum grown to a logarithmic stage, and performing fermentation at 20-30 DEG C; and after the fermentation is completed, performing centrifugation on the fermentation liquid, and drying the precipitate obtained after centrifugation at 50-60 DEG C to a constantweight to obtain the required single cell protein. The method only needs a period of biological reaction to treat the biogas slurry, has a low running cost and convenient operation, has an excellenttreatment effect on the biogas slurry, and can provide high economic value products, thereby providing an integrated and economic solution for biogas slurry treatment.

The invention discloses a high protein double protein milk beverage and a preparation method thereof. The high protein double protein milk beverage comprises pea protein isolate, micronized whey proteins, colloidal stabilizers, white sugar and water; the protein content of the double protein milk beverage is 4% to 8%, the mass content of a colloid stabilizer is 0.02% to 0.05%, and the mass contentof the white sugar is 1% to 5%. The preparation method for the high protein double protein milk beverage is also disclosed. The high protein double protein milk beverage is prepared by compounding pea proteins with the micronized whey proteins with low viscosity and low gelation property, based on the complementary amino acid of the plant-derived protein, solves the problem that a previous vegetable milk beverage is low in protein content and rough in taste, and is a high-protein, green, nutritious and healthy milk beverage.

The invention relates to a method for extracting human serum albumin from plasma and increasing the yield. By adoption of the method, the problems of extracting recovered human serum albumin from the plasma and increasing the yield can be effectively solved. The adopted technical scheme is as follows: the method comprises the following steps of: combination and melting of the plasma, preparation and pressing filtration of components I, II and III, preparation and pressing filtration of a component IV, preparation and pressing filtration of a component V, precipitation, dissolving and filtration of the component V, refined purification of the component V, ultrafiltration dialysis and concentration, preparation, pasteur inactivation, bacteria removal and filling, incubation and verification; the preparation and pressing filtration of the component IV comprise the following steps of: adding physiological saline with the temperature of 0 DEG C and the volume of 1 / 10 of that of the supernatant of the prepared components I, II and III, using acetate buffer solution with pH of 4.0 to adjust the pH value of the solution to 5.80-5.90, adding alcohol with the temperature of minus 15 DEG C and the volume concentration of 95%, and enabling the final volume concentration of the alcohol to reach 40%. The method has the advantages that the production process is smoother and simple, the yield of the human serum albumin is increased and the economic and social benefits are large.

The invention relates to cell culture and transplantation, in particular to a serum-free medium which is suitable for microencapsulated CHO cells, wherein, DMEM / F12 is taken as a basic medium; the characteristics of growth and metabolism of the microencapsulated cells are focused; and a certain proportion of insulin, soybean lecithin, Tween 80, Tropolone, FAC, sodium selenite, beta-mercaptoethanol and amino acid are added. The serum-free medium can promote vigorous growth of the microencapsulated CHO cells, and the cell density, the product expression and the activity all reach or are superior to the cell culture level of a serum medium, thereby the serum-free medium can replace the serum medium to be used for culture and transplantation of the microencapsulated CHO cells, greatly reduce the culture cost, and improve the biological safety of the microencapsulated animal cells.

The invention discloses a mudskipper breeding bait nourishing agent, which is characterized by the following: using plant protein, animal protein, ammonium nitrate, potassium dihydrogenphosphate and silicate to make the product; providing organic nourishment and growing environment.

The invention discloses green and healthy feed with the good fattening effect for a fattening period of sheep. The feed comprises raw materials in parts by weight as follows: 20-40 parts of cooked peanut cakes, 15-35 parts of corn, 20-36 parts of corn germ meal, 20-40 parts of wheat, 15-35 parts of fermented sesame cakes, 2-10 parts of grass meal, 8-16 parts of chrysanthemum meal, 5-15 parts of silkworm chrysalis meal, 10-20 parts of bone meal, 8-13 parts of brewer's grains, 10-25 parts of fermentation protein, 0.002-0.006 parts of tryptophan, 0.005-0.009 parts of lysine, 0.005-0.0085 parts of methionine, 0.007-0.0095 parts of threonine, 0.004-0.008 parts of glutamic acid, 1-3 parts of a Chinese herbal medicine additive and 2-7 parts of an attractant. The feed is green, healthy and balanced in nutrition and has the good fattening effect.

The present invention concerns the field of nutritional compositions and concerns a nutritional composition, especially an infant formula containing whey protein / milk protein concentrate solids rich in phospholipids, rich in MFGM for use in the prophylaxis and prevention of infectious morbidity, especially otitis. At the same time the use of antipyretics has diminished.

The invention discloses nutritionally balanced feed for the sheep fattening period. The nutritionally balanced feed comprises, by weight, 3-7 parts of sea-foam stone powder, 4-8 parts of lard oil, 1-1.8 parts of soybean oil, 4-12 parts of a composited coated material, 0.04-0.1 part of phytase, 0.06-0.12 part of rhizopus oryzae, 0.04-0.12 part of selenium-enriched yeast, 0.3-0.8 part of methionine, 0.12-0.2 part of lysine, 2-4 parts of Chinese herbal medicine additives, 15-30 parts of corn gluten meal, 10-16 parts of fish meal, 35-55 parts of corn, 10-20 parts of broussonetia papyrifera powder, 12-18 parts of wheat, 5-12 parts of hay meal, 10-20 parts of silkworm chrysalis, 4-12 parts of cotton seeds and 10-20 parts of sweet potato leaves. The nutritionally balanced feed is green and healthy, balanced in nutrition and good in fertilizer effectiveness.

The invention discloses a method for preparing sulphated polysaccharides from guts or gonads of abalone. The method comprises steps as follows: 1, the guts or the gonads of the abalone serving as raw materials are extracted with an alkaline method or an enzyme method, and an extracting solution is subjected to centrifugation; 2, supernatant liquor is adsorbed by ion exchange resin, the iron exchange resin is washed with water until a flow-out liquid is clarified, and then the flow-out liquid is eluted through two sodium chloride solutions with different concentrations and is collected; 3, desalting and concentration are performed with an ultrafiltration method, and then alcohol precipitation is performed; 4, protein is sequentially dissociated again with the alkaline method and the enzyme method; 5, the protein is removed through acid modulation, after oxidative decoloration, alcohol precipitation and precipitation dissolution are performed, salt is removed with a film filtration method, parts inside a film are collected and subjected to freeze drying, and then the abalone sulphated polysaccharides are obtained. According to the sulphated polysaccharides obtained with the method, the purity is high, the protein content is lower than 10%, a preparation condition is moderate, the damage to the structure of the abalone sulphated polysaccharides is little, and the product quality is good.

The invention relates to a processing method of a granny smith apple juice. The method is characterized in that polyphenol enzyme activity is reduced by pretreatment such as blanching; nitrogen is filled during a squeezing process, so that a juice is isolated from the oxygen as much as possible before enzymolysis, and a browning reaction is inhibited; pectase is added for enzymolysis so as to enhance clarity; pH value is reduced by the action of ascorbic acid and malic acid; and the color value of the juice is improved by ion-exchange fiber adsorption.

The invention discloses low-gluten beer and a preparation method of the low-gluten beer. The low-gluten beer per 1500L is mainly prepared from the following brewing raw materials: 28 to 36 kg of rice, 28 to 36 kg of buckwheat, 72 to 88 kg of barley malt of which the gluten protein content is less than or equal to 10 g / kg and 8 to 16 kg of high-aroma malt of which the gluten protein content is less than or equal to 20 g / kg. According to the low-gluten beer disclosed by the invention, a specific raw material-auxiliary material ratio is adopted, a special preparation technology is combined, the gluten protein content of the low-gluten beer is lower than 100 mg / kg, and the requirement of the CAC (Codex Alimentarius Commission) on products containing extremely-low gluten can be met; the taste of the low-gluten beer is almost not different from that of common beer.

The invention provides application of allyl methyl trisulfide in the preparation of a medicine and / or a health product for preventing and / or treating drug-induced liver injury. Allyl methyl trisulfidecan effectively reduce death rate caused by APAP and inhibit rise of serum transaminase activity and increase of liver coefficient due to APAP, and can obviously mitigate liver injury caused by APAP.

The invention discloses a method for producing a mung bean / pea starch. The method comprises the following procedures: soaking beans, grinding beans, primarily screening, separating, precipitating, taking out the pulp, secondarily screening, hydrocyclone separating and purifying, and vacuum dewatering. On the basis of producing starch by a conventional acidic steeping liquor processing, a hydrocyclone separation device and the vacuum dewatering technique are combined, thereby greatly increasing the efficiency of separating the starch and the efficiency of dewatering, reducing the protein level in the starch, increasing the whiteness of the starch, greatly increasing the purity of the starch, reducing the indexes such as COD in the sewage, and greatly shortening the time of separating the starch and dewatering.