Method for preparing sulphated polysaccharides from abalone

A technique for sulfated polysaccharides and abalone, applied in the field of preparing polysaccharides and preparing sulfated polysaccharides, can solve the problems of waste of biological resources, environmental pollution, discarding, etc., and achieve the effects of reducing the amount of ethanol, high purity, and guaranteed quality

Inactive Publication Date: 2015-04-29
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Abalone viscera, including gonads, are by-products of abalone processing. Currently, they are mostly treated as low-value feed or even discarded, resulting in waste of biological resources and environmental pollution.

Method used

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  • Method for preparing sulphated polysaccharides from abalone
  • Method for preparing sulphated polysaccharides from abalone
  • Method for preparing sulphated polysaccharides from abalone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A preparation method of abalone sulfated polysaccharide, comprising the following steps:

[0037] (1) Use 550 grams of abalone gonad freeze-dried powder as the raw material, after crushing, add 4% sodium chloride aqueous solution, adjust the pH of the sodium hydroxide solution to 8-9, heat and stir at 50-60°C for 2 hours; Serum;

[0038] (2) The supernatant obtained in the above steps repeatedly flows through the D204 strong basic anion exchange resin, so that the sulfated polysaccharide is adsorbed on the ion exchange resin, and the ion exchange resin is washed with water until the effluent is clear and colorless, and then used The 1.4mol / L sodium chloride solution of 4 times the resin volume circulates through the ion exchange resin, and the effluent is collected; the 3mol / L sodium chloride solution of 3 times the resin volume circulates through the ion exchange resin, and the effluent is collected. ; Merge the two effluents;

[0039] (3) Add 3 times the volume of 9...

Embodiment 2

[0045] A preparation method of abalone sulfated polysaccharide, comprising the following steps:

[0046] (1) Take 120 grams of abalone gonad freeze-dried powder as raw material, after crushing, add pH8.0 Tris-acetic acid buffer solution, add 2% trypsin in dry weight of raw material and shake at 37°C for 4 hours, add 2% papain in dry weight of raw material at 50°C Shake for 4 hours, heat and stir at 100°C for 5 minutes; let stand to cool, then centrifuge, and take the supernatant;

[0047] (2) The supernatant obtained in the above steps repeatedly flows through the D204 strong basic anion exchange resin, so that the sulfated polysaccharide is adsorbed on the ion exchange resin, and the ion exchange resin is washed with water until the effluent is clear and colorless, and then used The 1.4mol / L sodium chloride solution of 4 times the resin volume circulates through the ion exchange resin, and the effluent is collected; the 3mol / L sodium chloride solution of 3 times the resin vol...

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Abstract

The invention discloses a method for preparing sulphated polysaccharides from guts or gonads of abalone. The method comprises steps as follows: 1, the guts or the gonads of the abalone serving as raw materials are extracted with an alkaline method or an enzyme method, and an extracting solution is subjected to centrifugation; 2, supernatant liquor is adsorbed by ion exchange resin, the iron exchange resin is washed with water until a flow-out liquid is clarified, and then the flow-out liquid is eluted through two sodium chloride solutions with different concentrations and is collected; 3, desalting and concentration are performed with an ultrafiltration method, and then alcohol precipitation is performed; 4, protein is sequentially dissociated again with the alkaline method and the enzyme method; 5, the protein is removed through acid modulation, after oxidative decoloration, alcohol precipitation and precipitation dissolution are performed, salt is removed with a film filtration method, parts inside a film are collected and subjected to freeze drying, and then the abalone sulphated polysaccharides are obtained. According to the sulphated polysaccharides obtained with the method, the purity is high, the protein content is lower than 10%, a preparation condition is moderate, the damage to the structure of the abalone sulphated polysaccharides is little, and the product quality is good.

Description

technical field [0001] The invention relates to the technical field of preparing polysaccharides from marine animal processing by-products, more specifically, to the technical field of preparing sulfated polysaccharides from abalone gonads or organs. Background technique [0002] Abalone is a traditional and precious food material in China, ranking first among the four seafood delicacies. The viscera of abalone, including gonads, are by-products of abalone processing. At present, they are mostly treated as low-value feed or even discarded, resulting in waste of biological resources and environmental pollution. Research by Zhu Beiwei and others found that sulfated polysaccharides in abalone viscera have anti-oxidation, anti-coagulation, and immune regulation effects, which are very valuable for development. [0003] Through the search, the following three published patent documents related to this patent application were found: 1. Zhu Beiwei et al. disclosed the extraction m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 宋爽黄璐温成荣王风雅程沙沙李德阳朱蓓薇
Owner DALIAN POLYTECHNIC UNIVERSITY
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