Joint preparation method of polysaccharide and protein peptide

Abstract

The invention relates to a joint preparation method of polysaccharide and protein peptide. The joint preparation method comprises the following steps: mincing abalone viscus wet materials, mixing and cooking with water; performing primary enzymatic hydrolysis by using alkaline protease; performing membrane separation by using the obtained enzymatic hydrolysate; performing secondary enzymatic hydrolysis on obtained trapped fluid by using flavorzyme; performing membrane separation; performing freeze-drying, spray drying or alcohol precipitation and vacuum drying on the obtained trapped fluid to obtain the abalone viscus polysaccharide; merging the trapped fluid with permeate liquid obtained by the primary enzymatic hydrolysis; performing freeze-drying or spray drying after reverse osmosis or vacuum concentration processing to obtain the abalone viscus protein peptide. The joint preparation method has the advantages that the operation is simple and practical, both the polysaccharide and the protein peptide can be simultaneously prepared, and the production efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of polysaccharides and proteins, and in particular relates to a combined preparation method of polysaccharides and protein peptides. Background technique [0002] Fujian is a big province of abalone breeding. The annual output of abalone is nearly 100,000 tons, accounting for more than 80% of the total domestic output. With the rapid growth of market demand, the processing of dried and frozen abalone is developing rapidly. By-products such as internal organs weighing 1 / 4 are mainly used to produce low-value-added products such as feed at home and abroad. Most of the internal organs of abalone are not fully utilized, which not only wastes resources, but also causes environmental damage. pollute. The polysaccharides and proteins in the viscera of abalone account for about 20% and 50% of the dry matter respectively, and are excellent resources for processing biological polysacchari...

AI Technical Summary

Problems solved by technology

[0003] At present, the process of using abalone viscera to prepare polysaccharides and protein peptides is mainly to extract and prepare polysaccharides or protein peptides separately, lacking a joint preparation process, and the utilization rate of raw materials is low

At the same time, since the polysaccharides and proteins in abalone viscera mainly exist in the form of glycoproteins, the abalone polysaccharides prepared by hot water / alkaline extraction, single enzymatic hydrolysis or membrane separation technology all contain more protein components. As a result, the purity of Bao polysaccharide is low, and whether the Sevag method or trichloroacetic acid method is used for deproteinization, or hydrogen peroxide or activated carbon is used for decolorization, the yield of the product will be significantly reduced.

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