The invention discloses a device and a method for detecting the content of As (III) in a
water body based on a paper-based micro-fluidic
chip. The method is based on the chemical principle that
arsenite reacts with
potassium iodate under the acidic condition to generate an
iodine simple substance, and
rhodamine B is oxidized by the generated
iodine simple substance under the catalytic action of a
sulfuric acid medium and
potassium bromide, so that the
rhodamine B is subjected to a color
fading reaction. According to the device, a fluid channel is prepared on a
filter paper material,
reagent dropwise adding areas and a reaction area are sequentially arranged, corresponding reagents are dropwise added into the
reagent dropwise adding areas in sequence, then the reagents are converged to a
main channel step by step through a micro-
fluidic channel and are communicated to the reaction area, and the reagents are mixed in the micro-
fluidic channel and subjected to a
chromogenic reaction in the reaction area. The method comprises the following steps: shooting an image of a reaction area, extracting color parameters by using
image processing software, and performing contrast calculation with a pre-established standard working curve to obtain the content of As (III) in the
water sample. The technical problems that in the prior art, operation steps are complex, the detection period is too long, the detection portability of the
arsenic content in the
water body is poor, and the detection cost is too high are solved.