14 results about "Organic arsenic" patented technology

The invention discloses a super microwave digestion and conversion rate determination method for organic arsenic in animal and plant samples, which comprises the following steps: adding a mixed solution of nitric acid and sulfuric acid into a sample, and performing digestion and cooling by adopting super microwaves to prepare a sample solution; the super microwave digestion conversion rate of the organic arsenic in the animal and plant samples is measured by using inductively coupled plasma mass spectrometry (ICP-MS) and hydride generation-atomic fluorescence spectrometry (HG-AFS). The method has the advantages of simple process, adoption of one-step digestion with super microwaves, no need of repeated acid addition, reduction of energy consumption and cost, short time of super microwave digestion, high conversion rate of super microwave digestion, facilitation of realization of rapid detection, and meeting of requirements of basic food detection.

The invention belongs to the technical field of microorganisms, and provides a gene combination for degrading organic arsenic, an engineering bacterium and application of the gene combination. The gene combination comprises a nitroreductase gene BsntrA, an arsenate reductase gene ArsC2 and an efflux pump gene BsexpA which are from brevundimonas M20, the gene combination is used for constructing a recombinant expression vector and transforming the recombinant expression vector into a host cell, the obtained gene engineering cell forms an organic arsenic reduction-secretion system through protein expressed by the gene, nitro reduction, arsenic (V) reduction and HAPA (III) secretion of roxarsone are achieved, the degradation rate of the gene engineering cell to roxarsone in wastewater reaches 75.6%, and the gene engineering cell has a good application prospect. The drug resistance to roxarsone is obviously improved, and the compound can be efficiently used for removing organic arsenic in pollutants.

The invention discloses a novel method for extracting and separating euphausia superba oil. The krill meal contains substances such as krill phospholipid, krill oil, fatty acid, astaxanthin, small molecule polypeptide, organic arsenic, basic nitrogen with heavy fishy smell and the like. The process of the substances or impurities from the shrimp powder to the solvent belongs to diffusion control, namely the lower the content in the solvent is, the faster the diffusion is, and the minimum amount of the needed solvent is, the higher the extraction yield is. Therefore, a percolation method is most suitable for extracting the shrimp oil, the shrimp powder is always in contact with a fresh solvent, and the maximum concentration difference, namely the maximum diffusion power, is kept. The percolation process is similar to the chromatography process, different substances are different in sequence when eluted due to different molecular structures, and the krill phospholipid and the oil are enriched respectively. When the same shrimp powder is used, compared with an extraction stirring method and a percolation method, the percolation method has the advantages that the solvent dosage is nearly half less, and the shrimp powder can be completely extracted only by absolute ethyl alcohol with the volume 6 times that of the shrimp powder. And the yield is 1-2% higher than that of a stirring method. If a product containing 56% of phospholipid needs to be produced, extraction can be completed only by absolute ethyl alcohol with the volume 2-3 times that of the mass of the shrimp meal. In the percolation process, time difference exists between extraction (or elution) of the krill phospholipid and extraction (or elution) of the krill oil, and krill oil products with different phospholipid contents can be conveniently obtained by utilizing the characteristic.

The present invention belongs to the field of medicine and chemical industry, and relates to an organic arsenic compound DMMTA V The application of DMA in the preparation of drugs for treating skin inflammation comprises the following steps: V The mixture was dissolved in ultrapure water and anhydrous sodium sulfide, reacted in an ice bath, and concentrated sulfuric acid was slowly added dropwise. The mixture was magnetically stirred under argon protection. After the reaction, pre-cooled methanol was added for crystallization. HCl was added to adjust the pH. The mixture was dehydrated in a water bath. Chloroform was used for extraction. After drying and evaporation, methanol / water was added for cooling and crystallization. The organic arsenic compound DMMTA was obtained after vacuum drying. V .DMMTA V It can not only relieve inflammation by increasing MMP‑9 and reducing TIMP‑1 to promote the reconstruction of the respiratory tract and lungs and participate in angiogenesis, but also reduce the excessive secretion of inflammatory factor 8 caused by lipopolysaccharide, reduce damage to human skin keratinocytes, and relieve skin inflammation.

This invention provides a method for preparing high-content krill phospholipids and removing organic arsenic using preparative high-performance liquid chromatography (HPLC). The method includes: S1, dissolving Antarctic krill oil; S2, separating Antarctic krill phospholipids and removing organic arsenic using preparative HPLC; and S3, concentrating the eluent to remove the solvent, obtaining Antarctic krill phospholipids with a total phospholipid content ≥60% and a total arsenic content <1 mg / kg. The chromatographic separation materials used in this invention are pure, and the solvents used are food-grade ethanol, n-hexane, etc., meeting food safety requirements. The process can be implemented using a fully automated preparative chromatography system, exhibiting good reproducibility. This method can obtain high-safety Antarctic krill phospholipid products with a total arsenic content of less than 1 mg / kg and a total phospholipid content ≥60%, ensuring that the total arsenic content of the product meets the safety limits for health food raw materials. This satisfies the needs of high-end application scenarios such as infant and pregnant women's nutritional products and is beneficial for developing functional Antarctic krill phospholipid products.

The application discloses a copper residue-based amorphous composite material, which is prepared by the following steps: crushing and screening dried copper residue, calcining the screened copper residue at 450-550 DEG C, mixing the calcined copper residue, manganese salt and water, adding potassium permanganate solution into the mixture, uniformly mixing the mixture, heating the mixture in a water bath at 45-55 DEG C, and separating the reaction product into solid and liquid, and then washing, drying and grinding the solid to obtain the copper residue-based amorphous composite material; the material has the advantages of high specific surface area, many reaction active sites, good oxidation and adsorption performance, high metal atom utilization rate and stable structure, and can be widely applied to oxidizing and adsorbing organic arsenic; and the adsorption removal effect is good; and the application realizes resource utilization of solid waste and has great application prospect in the field of environment.

This invention provides a standard quality control sample for edible fungi matrix containing multiple arsenic forms, its preparation method, and its application, relating to the field of standard material technology. The invention involves preparing a powder from dried edible fungi samples (containing monomethylarsenic acid, dimethylarsenic acid, arsenic betaine, and arsenite) to obtain a powder sample. An aqueous solution of arsenate is added to the powder sample, and the resulting wet powder sample is then sequentially dried, pulverized, and mixed to obtain a standard quality control sample for edible fungi matrix containing multiple arsenic forms. This invention uses dried edible fungi samples as raw materials, and the prepared edible fungi matrix standard quality control sample is easy to preserve stably, and temporary sampling is less likely to induce arsenic form conversion. The method of this invention is simple, and the prepared edible fungi matrix standard quality control sample exhibits good uniformity and excellent stability. It can be used for the accurate quantification of inorganic arsenic in edible fungi, and through the qualitative determination of organic arsenic, it can effectively prevent false positives in inorganic arsenic results, effectively improving the accuracy and reliability of the detection results.

The invention belongs to the field of medicine and chemistry and chemical engineering, and relates to an application of an organic arsenic compound DMMAV in preparation of a medicine for treating scytitis, the preparation method comprises the following steps: dissolving DMAV and anhydrous sodium sulfide in ultrapure water, reacting under an ice bath condition, slowly dropwise adding concentrated sulfuric acid, magnetically stirring under the protection of argon, and carrying out suction filtration to obtain a filtrate; after the reaction is finished, pre-cooled methanol is added for a crystallization reaction, HCl is added for adjusting the pH value, a water bath is used for promoting a dehydration reaction, chloroform extraction, drying and evaporation concentration are performed, methanol / water is added for cooling crystallization, and vacuum drying is performed to obtain the organic arsenic compound DMMAV. The DMMAV can improve MMP-9 and reduce TIMP-1 to promote reconstruction of respiratory tracts and lungs, participate in angiogenesis and the like to relieve inflammation, and can also relieve damage to human skin keratinocytes and relieve skin inflammation by reducing excessive secretion of inflammatory factors 8 caused by lipopolysaccharide.

The invention discloses a six-membered cyclic organic arsenic compound and a preparation method thereof, a biotin-modified nano material and an antitumor drug, and belongs to the technical field of medicinal chemistry. The structural formula of the six-membered cyclic organic arsenic compound is as follows: # imgabs0. The six-membered cyclic organic arsenic compound synthesized by the invention has a symmetrical and regular geometric structure, and is beneficial to keeping the stability of the structure; the structure of the compound is small, exquisite and simple, the steric hindrance with a specific binding site is small, targeted binding with a protein target is facilitated, and the toxicity is lower while the biological activity of arsenic is kept. The biotin-modified nano material prepared from the organic arsenic compound can improve the enrichment degree of tumor tissues, trigger intracellular biochemical reaction, improve the antitumor activity in tumor-bearing mice and reduce toxic and side effects.

The invention discloses ferrous sulfide loaded iron modified charcoal as well as a preparation method and application thereof. The preparation method comprises the following steps: S1, dissolving a modifier and an iron salt in water, adding a charcoal precursor, or dissolving FeCl3 in water, adding thiophene-2, 5-dicarboxylic acid and 2-amino p-benzoic acid, reacting at 120-180 DEG C for 4-12 hours, cooling, washing, recovering a black solid, and drying; s2, reacting the black solid for 1-4 hours at 700-900 DEG C under the protection of inert gas, cooling to recover the solid, and washing to obtain carbon-coated monatomic iron; s3, under the protection of inert gas, dissolving FeSO4. 7H2O in the degassed water to obtain a solution A, and adding the carbon-coated monatomic iron to obtain a solution B; s4, dissolving Na2S in the degassed water to obtain a solution C, and dripping the solution C into the solution B under the protection of inert gas to obtain a black solution; and S5, centrifuging, washing and freeze-drying the black solution, grinding and collecting under the protection of inert gas to obtain the ferrous sulfide loaded iron modified biochar which can realize synchronous and rapid removal of organic arsenic, As (V), As (III) and phosphorus.

The application discloses a metal-doped mesoporous titanium dioxide composite material and a preparation method and application thereof. The material is prepared by the following steps: dropping a small amount of titanium chloride into ice to form a solution, stirring and adding a small amount of metal chloride (Cu, Fe), adding strontium chloride, dropping potassium hydroxide solution and stirring to obtain a slurry, placing the slurry into a high-temperature and high-pressure kettle, rapidly heating to 100-105 DEG C, heating for a period of time, washing the solution to neutral pH by centrifugation after cooling, and drying to obtain metal-doped strontium titanate. A small amount of metal-doped strontium titanate is taken into a high-pressure kettle, pure water and concentrated hydrochloric acid are added, the mixture is naturally cooled after high-temperature reaction for a period of time, the slurry after cooling is washed to neutral pH by a centrifugal machine, and the slurry is placed into a freeze dryer to be freeze-dried to obtain the mesoporous titanium dioxide composite material. The material can efficiently adsorb and remove pollutants (such as organic arsenic) in water, and realizes deep purification of water body organic pollutants.

The invention relates to a novel organic arsine compound CZ2 and an application thereof in tumor resistance. The organic arsine compound CZ2 disclosed by the invention not only shows remarkable anti-DLBCL activity in an in-vitro experiment, but also can effectively induce tumor cell apoptosis; in an animal model, the compound also shows a good anti-tumor effect, has no obvious toxic or side effect and has an important clinical application value. As an epigenetic regulating agent, CZ2 not only shows good anti-tumor activity to DLBCL, but also has the characteristics of low toxicity and high selectivity, and has clinical transformation potential. Besides, the CZ2 can also be applied to other malignant tumors (such as multiple myeloma and T cell lymphoma) depending on an epigenetic mechanism, or can be combined with existing targeted drugs and immunotherapy, so that the comprehensive curative effect of tumor treatment is improved.

The application discloses a kind of microbial inoculant and its application in arsenic pollution repair, the microbial inoculant includes humic acid reducing bacteria and humic acid, humic acid reducing bacteria is any one of Shewanella oneidensis MR-1 or Geobacter sulfurreducens, humic acid is any one of soil humic acid, water humic acid or coal humic acid.The microbial inoculant prepared by the application is loaded on humic acid by humic acid reducing bacteria, utilizes aerobic-anaerobic dynamic change, periodically generates ·OH, efficiently oxidizes As (III) to As (V) or organic arsenic, and also utilizes ·OH to promote carbonization reaction of humic acid to generate graphite carbon, further enhancing the adsorption and fixation effect of arsenic, without additional oxidizing agent, green and economical, realizing the synergistic effect of microorganisms and humic acid, realizing arsenic oxidation and graphite carbon adsorption integration, with high and lasting repair efficiency.

The present invention provides a preparation method and application of a mesoporous polydopamine nano-antibacterial agent loaded with an organic arsenic agent. First, utilizing the characteristic of NO donors releasing antibacterial NO upon heating, the NO donors are loaded onto the surface of mesoporous polydopamine nanoparticles with a photothermal conversion function to obtain modified mPDA@NO nanoparticles. Then, using the modified mPDA@NO nanoparticles as carriers, the organic arsenic fungicide is adsorbed into the mesopores of the nanocarriers. After reaching a specific position and under near-infrared light irradiation, the nanoparticles are not only rapidly heated to achieve a bactericidal effect, but also promote the release of the organic arsenic fungicide and antibacterial gas NO. Under the synergistic antibacterial action of photothermal and chemical antibacterial drugs, the drug is precisely controlled and efficiently killed against drug-resistant bacteria.