157 results about "Cell density" patented technology

The invention provides a preparation method of an ADC foaming agent for improving foaming pore density, which comprises the following steps: step 1, mixing a modified inorganic nucleating agent with water according to a predetermined mass ratio, adding a dispersing agent, and uniformly mixing to prepare uniform and stable suspension slurry to prevent agglomeration of the nucleating agent; 2, when the conversion rate of the biurea reaches 80%-85%, adding the suspension slurry, so that the inorganic nucleating agent is uniformly attached to the surface of the ADC crystal; and 3, continuing the oxidation reaction until the biurea conversion rate reaches 92% or above, and then washing, centrifuging and drying to obtain the ADC foaming agent. The ADC foaming agent for improving the foaming pore density is prepared by the method. The inorganic nucleating agent is added in the stage of preparing the ADC foaming agent, and compared with the inorganic nucleating agent added in the subsequent foam material preparation process, the density of foaming pores can be remarkably improved.

The invention discloses an expression system suitable for short-chain non-specific peroxidase and application of the expression system, and belongs to the technical field of enzyme engineering and genetic engineering. According to the expression system disclosed by the invention, a nucleotide sequence of short-chain non-specific peroxidase with SUMO and 8HIS tags is connected to a pET28a vector to obtain a recombinant vector, and escherichia coli BL21 (DE3) is transformed to obtain a genetically engineered bacterium; genetically engineered bacteria are subjected to two-stage temperature control culture in a ZYM5052 liquid culture medium, and meanwhile, 5ALA is added into the culture medium. According to the invention, an escherichia coli self-induction system is used, so that a shake-flask culture can grow at high density in a short time, the culture medium contains rich nutrient substances, and monitoring of cell density and adding of IPTG are not needed in the expression process. Meanwhile, a heme precursor 5ALA is added into an escherichia coli self-induction culture medium, and finally the high-activity short-chain non-specific peroxidase is obtained.

Described herein are methods, systems, and programming for determining a tumor immunophenotype of an image of a tumor. Some embodiments include dividing an image into tiles depicting tumor epithelium and / or tumor stroma. For each tile, an epithelium-immune cell density and a stroma-immune cell density may be calculated based on a number of immune cells identified in the tumor epithelium and the tumor stroma, respectively. Based on the epithelium-immune cell density and the stroma-immune cell density, an inflammation type of the type may be determined, and a tumor immunophenotype may be determined based on each tile's inflammation type.

The invention discloses a physiological microenvironment-based cell culture simulation system, which relates to the field of cell culture, and comprises a main control unit adopting an embedded microcontroller; the multi-mode environment simulation unit is connected with the main control unit and comprises a fluid shear force module and a mechanical stretching module; the multi-parameter environment monitoring unit is connected with the main control unit and comprises an optical sensor for monitoring cell density, a pH sensor for monitoring environment parameters and a temperature sensor; the culture chamber is used for accommodating a cell culture; the environment regulation and control unit is used for regulating culture environment parameters; the multi-modal environment simulation unit and the multi-parameter environment monitoring unit are integrated, and the main control unit optimizes a multi-target control strategy in a manner of dynamically adjusting a weight coefficient based on cell growth state parameters monitored in real time, so that accurate and adaptive closed-loop management of cell culture is realized.

Provided are methods for the creation of ultra-high density cryopreserved cell banks. In certain embodiments, these methods employ altered perfusion culture techniques that allow for production of ultra-high density cell cultures that can be cryopreserved at unexpectedly high cell densities without the need for any cell concentration steps, while retaining excellent cell viability and quality.

The invention relates to the technical field of electromagnetic compatibility (EMC) design of electronic circuits, in particular to a method suitable for a flow cytometer and capable of reducing interference of clock signals on EMC. The method comprises the steps that S100, clock circuit components are concentrated in a 5mm * 5mm area of the top layer of a PCB, routing is limited to a second layer through buried holes, and fundamental frequency is dynamically set; s200, laying copper in a key area for grounding, and adding a ground bounce suppression array to three layers; s300, arranging an upper ground copper layer and a lower ground copper layer by using a flexible circuit board; s400, covering the top circuit with a ferrite alloy cover, and welding and grounding; and S500, the flow velocity and the cell density are collected in real time, the clock frequency is dynamically adjusted, a frequency stabilization mechanism exists in an extreme scene, radiation is effectively reduced, and the detection precision and stability are improved. According to the method, the EMC interference of the clock signal of the flow cytometer is reduced from multiple dimensions of source inhibition, shielding protection, connection optimization and cooperative control.

The invention discloses a preparation method and application of high-activity recombinant collagen, and relates to the field of biological medicine, and the preparation method comprises the following steps: S1, providing engineering bacteria containing a recombinant collagen expression vector, and performing seed culture; s2, inoculating the seed solution into a fermentation culture medium for fermentation culture; s3, in the fermentation process, when the thallus density reaches a preset value, a signal factor without an animal source is added into a fermentation system, and the signal factor is bionic polypeptide; s4, after the signal factors are added, the fermentation conditions are adjusted, and target collagen expression is induced; according to the preparation method and the application of the high-activity recombinant collagen, an endogenous metabolic network of bacteria is actively regulated and controlled by accurately adding animal-source-free bionic polypeptide signal factors in the fermentation process of engineering bacteria, and correct folding and specific post-translational modification of target collagen are induced; therefore, the hydroxylation rate and the biological activity of the recombinant collagen are improved, and the structure and the function of the recombinant collagen are closer to those of natural collagen of a human body.

The invention discloses a method for researching influence on hemolytic activity of marine microalgae based on a transcriptome technology, and relates to the field of hemolytic activity analys.The method comprises the steps that pretreatment is conducted on the marine microalgae based on culture requirements, and cystic morphological cell density and hemolytic activity of the marine microalgae are measured according to experimental design rules after pretreatment is completed; carrying out transcriptome sample collection operation by utilizing the marine microalgae subjected to pretreatment, constructing a library according to a transcriptome sample collection result, and obtaining a gene function annotation and a differential expression gene result; the growth conditions of the marine microalgae under different temperature conditions are analyzed according to cystic morphological cell density and hemolytic activity, and the regulatory gene influencing the hemolytic activity of the marine microalgae is obtained by combining gene function annotation and differential expression gene results. According to the method, the hemolytic activity of the marine microalgae cultured under different temperature conditions is extracted, so that the aim of researching related metabolic pathways possibly participating in toxin synthesis and hemolytic activity regulation is fulfilled.

This invention discloses a polyetheretherketone (PEEK) / aramid fiber composite microporous material and its molding method, belonging to the field of engineering plastics processing. The invention first modifies aramid fibers by amylation and prepares carboxylated PEEK. Covalent bonding between the amino and carboxyl groups is achieved through a chemical reaction, resulting in aramid fibers coated with PEEK. These fibers are then mixed with PEEK resin, a melt flow modifier, and additives to obtain a composite material. The composite material is then subjected to biaxial thermo-stretching orientation to improve its transverse / longitudinal orientation and crystallinity. Finally, the composite microporous material is obtained through supercritical CO2 microporous foaming. Because the foaming temperature is lower than the thermo-stretching orientation temperature, the biaxial orientation structure of the composite material is well preserved, and the well-dispersed aramid fibers effectively improve the cell wall strength, inhibit cell growth and merging, significantly increase cell density, and drastically reduce cell size, thereby constructing a high-strength, heat-insulating PEEK composite microporous material.

An object of the present invention is to provide a cell culture method and a production method for a product, in which an occurrence of aggregates in foam is suppressed.According to the present invention, there is provided a cell culture method of culturing cells at a cell density of 30×106 cells / mL or more and 400×106 cells / mL or less in a culture solution, the method including: step A of adjusting a poloxamer concentration in a foam liquid constituting foam to be 6 times or less a poloxamer concentration in the culture solution; or step B of adjusting a viscosity of the foam liquid constituting the foam to be 1.5 mPa·s or less under conditions of 35° C. or higher and 38° C. or lower.

An exemplary method to design an integrated circuit includes generating a first floorplan for an integrated circuit, the first floorplan including a plurality of first rows having a first cell height and a plurality of second rows having a second cell height. Generating the first floorplan includes organizing the first rows and the second rows in a first row pattern. The method also includes modifying the first floorplan based on a predetermined cell density of a plurality of first cells and a plurality of second cells to generate a second floorplan, the first cells having the first cell height and the second cells having the second cell height. Modifying the first floorplan includes organizing a set of first rows and a set of second rows in a second row pattern. The second row pattern has X' first rows and Y' second rows, where X' and Y' are positive integers.

The invention discloses a haematococcus culture dissolved oxygen coupling regulation and control method for a corrosion-resistant photoreactor, relates to the field of bioengineering, and builds a photoreactor made of a corrosion-resistant material and a matched regulation and control system; preparing a special improved BG-11 culture medium for haematococcus and injecting the culture medium into the photoreactor; oxygen transfer coefficients under different ventilation rates are calibrated through a dynamic method, a ternary equation of light intensity, cell density and oxygen production rate is fitted by combining photosynthetic characteristics of haematococcus, and a basic model for dynamic prediction of dissolved oxygen is established. According to the method, the photoreactor made of a corrosion-resistant material and a matched regulation and control system are built, so that the interference of material dissolution on the culture medium is effectively avoided, and the stability of a haematococcus culture environment is guaranteed. Based on a ternary fitting equation of an oxygen transfer coefficient calibrated by a dynamic method, light intensity, cell density and oxygen production rate, a basic model for dynamic prediction of dissolved oxygen is constructed, and accurate coupling regulation and control of dissolved oxygen are realized by combining model prediction control and reinforcement learning agents.

The invention discloses an osteoclast culture method as well as a culture medium and application thereof, the culture method is based on primary monocyte extraction, inoculation is performed according to a specific density, then quantitative M-CSF is added, and culture is performed overnight; on the second day, when the cell confluence degree of the mononuclear bone marrow hematopoietic stem cells is 5-30%, supplementing quantitative RANKL into each hole, continuously culturing for 2-4 days, then changing the solution, and obtaining mature osteoclasts on the sixth-seventh day. According to the invention, the optimization relationship among the cell activity, the cell density and the culture medium in the primary culture process of the osteoclast is deeply studied, and from the perspective of cost reduction and efficiency improvement, the use cost of cell factors is remarkably reduced, the number of times of liquid change is reduced, and the traditional culture time of 10-12 days (calculated from cell extraction) is shortened to 6-7 days. The average diameter of the mature osteoclast cultured by the method is larger, the number of fused cell nucleuses is larger, the space proportion of the mature osteoclast is not less than 80%, and the cell quality is obviously higher than that in the prior art.

The invention provides an injection for inducing mesenchymal stem cells. The induced mesenchymal stem cell injection is prepared from induced mesenchymal stem cells modified by hepatocyte growth factors with the cell density being 1.0 * 10 < 5 >-2.0 * 10 < 7 > / ml, HSA with the mass concentration being 0.25%-6%, DMSO with the mass concentration being 5%-10%, glucose with the mass concentration being larger than 0 and smaller than 1%, compound amino acid with the concentration being 0.5-5 mg / mL and the balance compound electrolyte injection. And the induced mesenchymal stem cell injection has a pH value of 6.8 to 7.5. The induced mesenchymal stem cell injection is good in homogeneity, small in batch difference and stable in curative effect, can effectively promote damaged tissue repair, can be directly intravenously injected after cryopreservation and recovery, keeps high cell viability and drug activity, achieves an excellent treatment effect, has good room-temperature stability, is convenient to transport and store, and is suitable for clinical application. The method is suitable for different clinical application occasions, and the clinical application range of the mesenchymal stem cells is expanded.

The application relates to the fields of biological medicine and computing simulation, and specifically discloses an immune cell expansion process simulation and process parameter optimization system, which collects multiple groups of candidate process parameters, introduces process disturbance, generates a dynamic trajectory set of living cell density, viability and metabolic by-products, and converts the dynamic trajectory set into a first probability distribution; calculates an entropy regularization distribution distance between the first probability distribution and a second probability distribution corresponding to ideal expansion kinetics as a matching degree measurement; constructs a difference response surface with the candidate process parameters and the distance value as samples, takes the current optimal distance as a benchmark, and calculates potential expected values of each candidate parameter by using the response surface; iteratively selects the parameter with the maximum potential expected value until the distance reduction amplitude is lower than a threshold value, and outputs the optimal process parameter; the application can avoid local optimization, improve the global optimization, and reduce the simulation rounds through dynamic distribution matching and difference response surface iterative optimization.

This invention provides the principle and method for influencing cellular functional excitatory expression through misaligned distribution of acoustic wave frequencies, relating to the interdisciplinary field of biological signal processing. The principle and method for influencing cellular functional excitatory expression through misaligned distribution of acoustic wave frequencies includes the following steps: Step 1: Target cell pretreatment, selecting cells to be regulated, separating, purifying, and culturing to the logarithmic growth phase, adjusting the cell density to... 1×104‑1×106cells / cm2 The cells are placed in a biocompatible culture medium. The method of this invention significantly improves the specificity of regulation. Through a frequency-function specific matching mechanism, frequency groups targeting specific cellular functions are screened. Combined with a multidimensional orbital misalignment design to avoid frequency interference, each frequency precisely activates the corresponding signaling pathway. The single-function excitation expression level of cells is more than twice that of the traditional single-frequency method, and the non-specific functional excitation rate is reduced by more than 80%, while the efficiency of action is greatly improved.

The invention belongs to the field of biological medicine, and relates to a curcumin-loaded bone marrow mesenchymal stem cell source exosome which is obtained by the following steps: step 1, culturing BMSCs (bone marrow mesenchymal stem cells): simultaneously adding 10% of fetal calf serum and 1% of myclostreptomycin into an MEM culture medium, culturing at 37 DEG C under 5% CO2, and counting and subculturing when the cell density reaches 90%; 2, dissolving Cur in a DMSO solution, preparing a mother solution with the culture medium, and filtering and diluting to 30-50 [mu] g / mL; adding into BMSCs (bone marrow mesenchymal stem cells) with the cell density of 85-100%, and culturing for 12-36 hours; collecting supernate, and carrying out gradient centrifugation to obtain the mesenchymal stem cell source exosome loaded with curcumin. The invention provides a novel administration route which depends on the self function of the mesenchymal stem cell source exosome and the self function of the curcumin at the same time.

A method for large-scale expansion of natural killer (NK) cells in a cell culture medium according to the invention, comprising the steps of: adding said cells to a culture system comprising a growth medium supplemented with serum selected from the group consisting of human serum and autologous plasma; cRX 527 with an effective concentration is added; the cells are expanded and heated within the system wherein the effective concentration of CRX 527 in the growth medium is in the range of 0.01 [mu] g / ml to 10 [mu] g / ml, preferably 100 ng / ml, and the ratio between the cell density and the total volume of the growth medium is in the range of 1.5 to 2.

The invention belongs to the technical field of gene engineering, and particularly relates to a CHO cell transient transfection method and application thereof.The CHO cell transient transfection method comprises the following steps that subcultured CHO cells are resuspended through a transfection culture medium, the cell density in CHO cell suspension is 0.8 * 10 < 7 >-1.5 * 10 < 7 > cells / mL, a PEI transfection reagent containing 40 kDa linear PEI is used, plasmid DNA and the PEI transfection reagent with the mass ratio being 1: (1-6) are added into the CHO cell suspension for transfection, and transfection is conducted after the PEI transfection reagent is added into the CHO cell suspension; the addition amount of plasmid DNA in each milliliter of CHO cell suspension is 3.0-7.0 [mu] g, transfecting for 20-24 h, then adding a cell suspension culture medium, transfecting for 48-72 h, then repeatedly transfecting, and culturing to obtain the recombinant CHO cell. According to the method, the transfection efficiency can be improved, more cells for expressing the recombinant protein can be obtained, and the transient expression of the target protein can be remarkably improved.

The present invention relates to the use of online biomass capacitance monitoring in cultures as a way to control the growth of cells through the use of a temperature control loop. In certain embodiments, a biomass capacitance probe is used to measure the cell density, and a predetermined growth curve is used to adjust the temperature in the culture.

A process and system for producing an inoculum for downstream cell production is disclosed. The inoculum is produced in a perfusion bioreactor in which the nutrient media feed is increased as the biomass concentration increases within the bioreactor. A biomass sensor can be used to periodically or continuously monitor biomass concentration. This information can be fed to a controller for automatically increasing nutrient media feed rates in a manner that is directly proportional to producing an inoculum with an increase cell density. The process and system can also include an automated subsystem for maintaining constant volume levels within the perfusion bioreactor during the process.

The invention discloses an acetate-resistant domesticated algal strain HpAc50 and an application thereof. The algal strain is preserved in the China Center for Type Culture Collection on January 12, 2026, and the preservation number is CCTCC M 202690. The haematococcus pluvialis strain is obtained by carrying out acetate-resistant domestication on haematococcus pluvialis and can be cultured under the condition that the acetic acid concentration is 0-10g / L, and the yield of astaxanthin is effectively increased. The invention specifically provides three application conditions: illumination of 0-5000lux and a full-nutrient culture medium; illumination is performed at 0-5000 lux, a nitrogen-phosphorus-deficient culture medium is adopted, and an exogenous stimulant is added According to the present invention, with the application of the strain, the cell density, the biomass and the astaxanthin accumulation can be significantly improved under the conditions of illumination of 5000-15000 lux and nitrogen and phosphorus deficiency culture medium, such that the strain has strong acetate tolerance and strong production performance compared with the wild type strain, and is suitable for the large-scale astaxanthin production.

This invention relates to the field of wire and cable technology, specifically disclosing a composite sheath for cable that can be threaded through and split. The composite sheath, from the inside out, consists of a closed-cell foamed buffer layer, a flexible metal shielding layer, and a low-smoke halogen-free outer protective layer. It has an axially pre-compressed easy-tear cutting line (0.5-1mm wide, half the sheath wall thickness). The shielding layer, foaming layer, and outer protective layer are bonded together via heat sealing / co-extrusion, with an interlayer peel strength ≥2.0N / mm, and each layer is indestructible. The composite sheath length is customizable, with a maximum custom length of 3000 meters. The closed-cell foamed buffer layer has a cell density of 50-300 cells / cm³. 3 This invention combines shock absorption, heat insulation, and waterproofing functions. The flexible metal shielding layer (shielding attenuation ≥60dB) is interference-resistant and firmly bonded, while the low-smoke, halogen-free outer protective layer (oxygen index ≥35%) uses materials adapted to different scenarios. This invention solves the problems of existing composite sheaths being non-removable, easily peeling between layers, limited length, and single function. It is suitable for long-distance, multi-scenario cable protection, significantly extending cable lifespan.

The invention discloses a high-salt pharmaceutical wastewater-based low-cost halotolerant bacterium culture medium and an expanding culture method thereof, and belongs to the field of environmental protection and microbial fermentation. According to the culture medium, 90%-95% of pretreated high-salt pharmaceutical wastewater is used as a substrate and matched with industrial auxiliary materials such as industrial glucose and urea, the salinity of the pretreated wastewater is 20000-30000 mg / L, and the COD is 10000-20000 mg / L. The expanding culture method comprises the steps of strain activation, seed solution preparation, 2 m < 3 > batch scale expanding culture, harvesting and storage, microporous aeration and mechanical stirring are adopted to guarantee uniform dissolved oxygen, and a trehalose-glycerol composite protective agent is added. According to the method, the cost of a ton of fungicide is greatly reduced by using waste culture bacteria, the cell density of the fungicide is greater than or equal to 30,000,000 / mL, the 36h COD removal rate is greater than or equal to 70%, the activity attenuation is less than or equal to 5% after 30 days of refrigeration at 4 DEG C, the problems of high cost, difficulty in large-scale production and poor stability in the prior art are solved, and the method is suitable for biological enhancement treatment of various types of high-salt pharmaceutical wastewater.

The invention provides a preparation method and application of a saccharomyces cerevisiae strain with high flocculation efficiency, a specific gene is knocked out and transferred in by using a homologous recombination method through a gene engineering technology to modify a saccharomyces cerevisiae BY4741 strain, and the metabolic pathway and related performance of yeast are accurately regulated and controlled. The method comprises the following specific steps: knocking out the SNF11 gene of the saccharomyces cerevisiae BY4741 strain, restoring the SNF11-SS18 (QPGY) gene, and constructing to obtain the saccharomyces cerevisiae strain with high flocculation efficiency, so that the efficient aggregation of yeast cells in a fermentation tank is realized, the cell density is improved, the fermentation period is shortened, expensive equipment such as a centrifugal machine is avoided, the energy consumption is reduced, and the production cost is reduced. Therefore, the fermentation efficiency is remarkably improved, the production cost is reduced, and powerful technical support is provided for sustainable development of the alcoholic fermentation industry.

The invention discloses a PARP inhibitor sensitivity prediction method based on a specific cell cluster and application. The method comprises the following steps: acquiring an ovarian cancer tumor tissue sample of a subject; detecting whether a specific cell cluster exists in the sample or not, and determining the abundance of the specific cell cluster, wherein the specific cell cluster comprises an IFN epithelial cell cluster CN12 taking an IFN tumor cell as a center and / or a TAM enrichment cluster CN29 taking an M2 type tumor related macrophage as a center; according to the detection result, predicting according to at least one of the following rules: if the CN12 abundance before treatment is greater than 0.8%, the CN29 abundance before treatment is greater than 32.7%, the CN12 abundance after treatment is greater than 0.009%, the CN29 abundance after treatment is greater than 2.9%, or the SPP1 cell density before treatment is greater than 1.7%, predicting that the drug resistance is not sensitive or easily generated. The prediction model is established from a cell cluster space tissue level, and the method has the advantages of high prediction accuracy, flexible sample requirements, high operability and the like.

The invention discloses a method for removing harmful algae. According to the method, silicate hollow microspheres are selected for flotation of the harmful algae such as microcystis and anabaena. In order to enhance the effect of removing harmful algae, aluminum salt containing Al < 3 + > is added into the water body environment before the flotation process. The method is simple in technological process, low in economic cost, suitable for removing harmful algae with different cell densities and high in removal rate under different pH conditions. The used silicate hollow microspheres have low density and high strength, the surfaces of the silicate hollow microspheres have certain roughness, combination with the two microalgae is facilitated, the method causes less pollution to the environment, and the floating beads can be repeatedly used for 3-4 times.

The present invention addresses the problem of providing a cell culture method in which the generation of aggregates in foam is suppressed, and a method for producing a product. According to the present invention, provided is a cell culture method in which cells are cultured in a culture solution at a cell density of 30 * 106-400 * 106 cells / mL, said method comprising: a step (A) in which the poloxamer concentration of a foam solution constituting a foam is adjusted to 6 times or less of the poloxamer concentration in the culture solution; or a step B in which the viscosity of a foam liquid constituting the foam is adjusted to 1.5 mPa.s or less under the condition of 35-38 DEG C (inclusive).

The invention provides a prediction method of a cell culture cooling point and application thereof, and relates to the technical field of fermentation culture.The method comprises the steps that cell density data are obtained, the current specific growth rate mu current of cells is calculated after smoothing algorithm processing, and when mu current is smaller than or equal to a mu threshold value, it is judged that the cooling point is reached; the mu threshold value is equal to k * mu max; wherein the mu max is the maximum specific growth rate of the cells, the k is 0.4-0.6, and the mu max and the mu current are from the same batch of cells. According to the method, the specific growth rate mu of the cells is used as a credible process control decision variable for the first time, the hysteresis of a traditional living cell density method is overcome through robust data processing, prospective capture of the physiological state of the cells is achieved, and the product yield and the process robustness are remarkably improved.

This invention discloses a recombinant *E. coli* strain for producing phenyllactic acid, a lactate dehydrogenase mutant, a whole-cell catalytic method for preparing phenyllactic acid, and the application of the aforementioned recombinant *E. coli* strain or lactate dehydrogenase mutant in the catalytic preparation of phenylpyruvic acid from phenyllactic acid. This invention significantly improves catalytic efficiency by modifying the 52nd amino acid of lactate dehydrogenase through site-directed mutagenesis. After mutating glutamine (Q) to valine (V) at position 52 of the *Lactobacillus mucosa* lactate dehydrogenase, the catalytic efficiency is significantly improved even at low cell density (OD). 600 =15) and substrate 15 g / L conditions, almost complete conversion was achieved; and combined with high-density catalysis and surface display optimization, the yield of phenyl lactic acid was increased while the downstream separation and purification costs were significantly reduced, greatly enhancing the potential for industrial production.