413 results about "Cell density" patented technology

The present invention provides a serum-free culture medium for in vitro suspension culture of porcine muscle stem cells and its application, which can help porcine muscle stem cells expand and be suspended under serum-free and carrier-free conditions. Compared with traditional porcine muscle stem cell culture protocols and culture media, this serum-free suspension culture medium enables porcine muscle stem cells to be cultured continuously at high densities in both batch and fed-batch culture processes under serum-free and carrier-free suspension culture conditions, while maintaining high cell viability and achieving a peak cell density of 1×10 7 The beneficial effect of the present invention is to effectively solve the problem of serum-free and carrier-free efficient suspension expansion of cell cultured meat seed cells, and provide a technical and raw material basis for the industrial production of cell cultured meat.

The invention provides a serum-free culture medium for in-vitro suspension culture of porcine muscle stem cells and application of the serum-free culture medium. The serum-free culture medium can help the porcine muscle stem cells to be subjected to suspension enlarged culture under serum-free and carrier-free conditions. Compared with a traditional porcine muscle stem cell culture scheme and culture medium, the serum-free suspension culture medium has the advantages that high-density continuous culture of porcine muscle stem cells can be realized in a batch culture process and a fed-batch culture process under the serum-free carrier-free suspension culture condition, high cell viability is maintained, and the survival rate of the porcine muscle stem cells is increased. The peak density of the cells reaches 1 * 10 < 7 > cells / mL or above, and the cell viability is maintained to be 90% or above. The method has the beneficial effects that the problem of serum-free carrier-free efficient suspension amplification of the seed cells of the cell culture meat is effectively solved, and a technical and raw material basis is provided for industrial production of the cell culture meat.

According to the microscopic cell image tracking method and device based on the graph neural network, the electronic equipment and the storage medium provided by the invention, a training set cell image is acquired and a training label is marked, a cell segmentation model is trained according to the training set cell image, and other data sets are predicted according to the cell segmentation model; according to the tracking method provided by the invention, the dependence on manual design can be reduced, the feature expression ability can be improved, the relationship between the cells can be modeled through the graph structure, the tracking precision under the condition of dense and shielded cells can be improved, and the tracking accuracy can be improved. Meanwhile, in combination with time sequence data, the time continuity of cell movement is captured, and the tracking stability is improved.

The invention relates to the technical field of image processing, in particular to an induced pluripotent stem cell density detection method, computer equipment and a medium, and the method comprises the following steps: acquiring an induced pluripotent stem cell image; presetting a local area of each pixel point; obtaining a local brightness performance value according to the gray level distribution of the pixel points in the local area; obtaining local boundary ambiguity according to the gradient distribution characteristics; obtaining local form regularity according to the uniformity characteristic of gray level distribution in a pixel point local area; obtaining a density comprehensive performance value of each pixel point; clustering the pixel points to obtain a preset number of clusters; and respectively enhancing each cluster by adopting histogram equalization to obtain an enhanced induced pluripotent stem cell image, and carrying out density detection on the enhanced induced pluripotent stem cell image. The invention aims to enhance the induced pluripotent stem cell image and improve the accuracy of the cell density detection result.

The invention provides a preparation method of an ADC foaming agent for improving foaming pore density, which comprises the following steps: step 1, mixing a modified inorganic nucleating agent with water according to a predetermined mass ratio, adding a dispersing agent, and uniformly mixing to prepare uniform and stable suspension slurry to prevent agglomeration of the nucleating agent; 2, when the conversion rate of the biurea reaches 80%-85%, adding the suspension slurry, so that the inorganic nucleating agent is uniformly attached to the surface of the ADC crystal; and 3, continuing the oxidation reaction until the biurea conversion rate reaches 92% or above, and then washing, centrifuging and drying to obtain the ADC foaming agent. The ADC foaming agent for improving the foaming pore density is prepared by the method. The inorganic nucleating agent is added in the stage of preparing the ADC foaming agent, and compared with the inorganic nucleating agent added in the subsequent foam material preparation process, the density of foaming pores can be remarkably improved.

The present invention provides a system for differential delivery of live BALO. The system comprises: a carrier comprising a polysaccharide hydrocolloid; and BALO immobilized within the carrier wherein the BALO is present in a physiological state selected from the group consisting of leech plastid, leech cyst and AP, which is present at a cell density of at least about 1.0 * 108 PFU / g carrier wherein the polysaccharide hydrocolloid is in the form of a wet or dry gel, and wherein the carrier preserves the viability and lysolytic efficacy of the immobilized BALO. Further provided are methods for making the systems for differential delivery of live BALO, as well as methods for using the same, e.g., for treating phytopathogenic bacterial diseases in plants or crops.

A semiconductor device includes a gate structure including conductive layers and insulating layers alternately stacked with each other, channel structures passing through the gate structure and arranged in a first direction, a cutting structure extending in the first direction and passing through the channel structures, and a first slit structure passing through the gate structure and extending in a second direction crossing the first direction.

The invention discloses an expression system suitable for short-chain non-specific peroxidase and application of the expression system, and belongs to the technical field of enzyme engineering and genetic engineering. According to the expression system disclosed by the invention, a nucleotide sequence of short-chain non-specific peroxidase with SUMO and 8HIS tags is connected to a pET28a vector to obtain a recombinant vector, and escherichia coli BL21 (DE3) is transformed to obtain a genetically engineered bacterium; genetically engineered bacteria are subjected to two-stage temperature control culture in a ZYM5052 liquid culture medium, and meanwhile, 5ALA is added into the culture medium. According to the invention, an escherichia coli self-induction system is used, so that a shake-flask culture can grow at high density in a short time, the culture medium contains rich nutrient substances, and monitoring of cell density and adding of IPTG are not needed in the expression process. Meanwhile, a heme precursor 5ALA is added into an escherichia coli self-induction culture medium, and finally the high-activity short-chain non-specific peroxidase is obtained.

A cell processing system, fluidics cartridge, and methods for using actuated surface-attached posts for processing cells are disclosed. Particularly, the cell processing system includes a fluidics cartridge and a control instrument. The fluidics cartridge includes a cell processing chamber that has a micropost array therein, a sample reservoir and a wash reservoir that supply the cell processing chamber, and a waste reservoir and an eluent reservoir at the output of the cell processing chamber. A micropost actuation mechanism and a cell counting mechanism are provided in close proximity to the cell processing chamber. A method is provided of using the cell processing system to collect, wash, and recover cells. Another method is provided of using the cell processing system to collect, wash, count, and recover cells at a predetermined cell density.

Described herein are methods, systems, and programming for determining a tumor immunophenotype of an image of a tumor. Some embodiments include dividing an image into tiles depicting tumor epithelium and / or tumor stroma. For each tile, an epithelium-immune cell density and a stroma-immune cell density may be calculated based on a number of immune cells identified in the tumor epithelium and the tumor stroma, respectively. Based on the epithelium-immune cell density and the stroma-immune cell density, an inflammation type of the type may be determined, and a tumor immunophenotype may be determined based on each tile's inflammation type.

The invention provides a method for promoting synthesis of microcystis astaxanthin by adjusting the concentration of a stress substance, which comprises the following steps of: firstly, culturing microcystis algal bloom or microcystis aeruginosa to a logarithmic phase by adopting a BG11 culture medium under the conditions of specific temperature, illumination and shaking table rotating speed, and adjusting the cell density by centrifuging and resuspending; then, NaCl, KCl, beta-violet ketone, longifolene and H2O2 with different concentrations are added into the algae liquid respectively, control is set, through multi-dimensional index measurement, the algae cell density, the ROS level, the photosynthetic pigment content, the photosynthetic performance and the level of astaxanthin and a precursor thereof are monitored when treatment is carried out for 0 day, 2 days, 4 days and 6 days, the expression quantity of astaxanthin biosynthesis related genes is further analyzed, and the astaxanthin biosynthesis related genes are obtained. Therefore, the optimal concentration of NaCl, KCl, beta-violet ketone, longifolene and H2O2 is determined, the effect of promoting astaxanthin synthesis is compared, and a theoretical basis and a precise regulation and control method are provided for industrial production of astaxanthin by using microcystis.

The present invention relates to a labyrinth seal for a turbine engine, in particular of an aircraft, comprising a rotor element and a stator element extending around the rotor element, the rotor element being suitable for rotating relative to the stator element about an axis of rotation having an axial direction (DA), the rotor element comprising an annular lip having an outer radial end extending towards an abradable element (57) carried by the stator element, the outer radial end of the annular lip having a corrugation in the axial direction (DA) and a non-zero axial expanse (E5) associated with the corrugation, the abradable element (57) comprising a plurality of cells (50a, 50b) arranged adjacent to one another along the axial direction (DA) and an ortho-radial direction (O), the cells (50a, 50b) comprising walls which extend in an essentially radial direction, the cells being distributed with a first cell density in a first densified annular zone (Z51) of the abradable element, said densified annular zone (Z51) being located opposite the radial end of the lip, said densified annular zone having an axial expanse less than or equal to the axial expanse of the outer radial end of the lip, the cells being distributed according to a reference density of cells outside said first zone, the first density being greater than the reference density.

The invention relates to a method for producing an antibody by improving cell inoculation density through seed perfusion culture and application. In N-1 seed cell culture, through a perfusion culture process mode, especially according to the cell density, the perfusion rate is adjusted to timely increase nutrient substances in a cell culture system and discharge metabolic wastes in cell culture to increase the density of living cells, so that the initial density of N-generation cells is greatly improved. The method is convenient to amplify and simple to operate, the antibody yield can be increased in the same culture period, or a target product can be obtained in a shorter culture period, and the production cost is greatly reduced.

The present application provides a method for detecting pseudo-lesions of the fundus, comprising: performing image feature analysis of the lesion area according to preliminary lesion markings, locating and evaluating the cell density and the degree of vascular leakage, and determining the risk of disease aggravation; predicting the tortuosity and vascular fragility of new blood vessels and evaluating the probability of complications in patients according to morphological processing and feature analysis of multi-phase vascular images; predicting the risk of serious complications according to the lesion markings and vascular feature analysis of the patient, combined with blood test results, and re-identifying and excluding pseudo-lesion images that are mistaken for lesion images; comparing the feature changes of pseudo-lesions and real lesion areas according to time series analysis of fundus disease images, evaluating the disease development path, and performing early intervention on the disease; segmenting the new blood vessel area based on the use of sensitive index filtering technology, and evaluating the risk of disease aggravation through vascular feature statistics.

A non-volatile memory with a 3D cross-point architecture and twice the cell density is provided, in which vertically stacked word lines run in one plane (i.e., parallel) with the substrate, and bit lines run perpendicular to the vertically stacked word lines. The vertically stacked word lines are arranged in a structured dielectric material stack, which has alternating first dielectric material layers and recessed second dielectric material layers. The first dielectric material layers vertically separate the respective word lines within each vertical stack of word lines, and the recessed second dielectric material layers are arranged laterally adjacent to the word lines. A dielectric switching material layer is placed between each word line-bit line combination.Some of the bit lines are arranged in the dielectric material stack, and some of the bit lines are arranged in an interlayer dielectric material layer.

The invention relates to the technical field of cell culture, and particularly provides an NK (Natural Killer) cell serum-free culture solution which is prepared from 24 percent by mass of traditional Chinese medicine extract and the balance of serum-free culture solution, the culture method of the NK cells comprises the following steps: adding the NK cells into a culture bottle, sampling and counting, diluting the cell density to 1 * 10 < 6 > / mL by using the serum-free culture solution of the NK cells, culturing in an incubator with the temperature of 37 DEG C and the CO2 content of 5% for 15 days, sampling and counting, circularly placing in a 326 mT magnetic field for 540 seconds and a 383 mT magnetic field for 120 seconds during culture, and keeping the two magnetic field environments at an interval of 0.5 hour. The serum-free culture device has a unique serum-free culture-magnetic field system, so that the multiplication capacity and killing capacity of the NK cells are not influenced or even improved while serum is not used.

To provide a moisture absorption device for a humidifier that can efficiently regenerate a moisture absorbing material-containing layer without using a heater, and can be reduced in size by reducing components constituting a dehumidifier.SOLUTION: A moisture absorption device 1 for a dehumidifier is provided with a honeycomb structure which has an outer peripheral wall 10 and a bulkhead 13 which is arranged inside the outer peripheral wall 10 and which sections and forms a plurality of cells 12 constituting a flow path extending from a first end face 11a to a second end face 11b, and a moisture absorbing material-containing layer 20 formed on a surface of the bulkhead 13. A thickness of the bulkhead 13 of the honeycomb structure is 0.089-0.140 mm and a cell density thereof is 62.0-93.0 cell / cm2.SELECTED DRAWING: Figure 1A

The invention discloses a physiological microenvironment-based cell culture simulation system, which relates to the field of cell culture, and comprises a main control unit adopting an embedded microcontroller; the multi-mode environment simulation unit is connected with the main control unit and comprises a fluid shear force module and a mechanical stretching module; the multi-parameter environment monitoring unit is connected with the main control unit and comprises an optical sensor for monitoring cell density, a pH sensor for monitoring environment parameters and a temperature sensor; the culture chamber is used for accommodating a cell culture; the environment regulation and control unit is used for regulating culture environment parameters; the multi-modal environment simulation unit and the multi-parameter environment monitoring unit are integrated, and the main control unit optimizes a multi-target control strategy in a manner of dynamically adjusting a weight coefficient based on cell growth state parameters monitored in real time, so that accurate and adaptive closed-loop management of cell culture is realized.

Bioreactors and methods for growing a chemoautotrophic culture of a microorganism, such as a hydrogen-oxidizing or carbon monoxide-oxidizing microorganism, are provided. The bioreactors and methods provide for enhanced growth and productivity of microorganisms that use gaseous sources of carbon and energy and provide an environment for carbon fixing to produce organic molecules of interest and / or biomass. The present bioreactors and methods achieve enhanced growth with respect to cell density, culture duration and / or growth rate, while maintaining safe operating conditions.

A non-volatile memory having a 3D cross-point architecture and twice the cell density is provided in which vertically stacked word lines run in plane (i.e., parallel) to the substrate and bit lines ru

The invention discloses a construction method of a tumor-associated meningeal metastasis model, and particularly relates to the technical field of biology. The method comprises the following steps: treating tumor cells according to a cell passage mode and centrifugal parameters, adding a serum-free culture medium, centrifuging again, adjusting the cell density by using a serum-free fresh culture medium, and sub-packaging to obtain a tumor cell suspension; the tumor cell suspension is injected into the L4-L5 intervertebral space of the mouse. After injection, the mouse resuscitates and normally diets; the growth of the tumor is monitored through a small animal living body imaging technology, and the dissected organ is subjected to in-vitro imaging to judge the invasion range of the tumor. The mouse tumor meningeal metastasis model is constructed in a simple, repeatable and efficient manner.

The invention provides a method for culturing beneficial algae by resource utilization of cyanobacterial blooms, which comprises the following steps: 1, obtaining cyanobacterial (microcystis) blooms, and concentrating the cyanobacterial (microcystis) blooms until the concentration of chlorophyll a is 80-150mg / L; 2, carrying out continuous hydrodynamic disturbance on the high-concentration cyanobacterial bloom, wherein the disturbance intensity is suitable for preventing the cyanobacterial bloom from blackening and stinking; 3, continuously disturbing for 20-60 days to obtain cyanobacterial bloom which turns light yellow green or white; 4, tap water is used for diluting cyanobacterial blooms with changing colors, the concentration during dilution is 0.5-2.3 mL / cm < 2 >, and beneficial algae such as diatom and green algae are inoculated for culture under the conditions of normal temperature and weak light; and 5, when the culture is performed for 12-30 days, the growth density of the beneficial algae reaches the highest peak, and the cell density of the beneficial algae can reach up to 2.5 * 10 < 6 >-5.6 * 10 < 7 > cells / cm < 2 >. According to the method, algal toxin can be removed, beneficial algae such as diatom and green algae can be cultured through resource utilization of the cyanobacterial bloom, and a new way is provided for control and resource utilization of the cyanobacterial bloom.

A non-volatile memory having a 3D cross point architecture and double cell density is provided in which vertically stacked word lines extend coplanar (i.e., parallel) with the substrate, and bit lines extend perpendicular to the vertically stacked word lines. The vertically stacked word lines are in a patterned stack of dielectric material that includes alternating first layers of dielectric material and recessed second layers of dielectric material. A first dielectric material layer vertically separates each word line within each vertical stack of word lines, and a recessed second dielectric material layer is positioned laterally adjacent to the word lines. The dielectric switch material layer is located between each word line-bit line combination. Some of the bit lines are in the dielectric material stack, and some of the bit lines are in the interlayer dielectric material layer.

The present invention provides a continuous upstream manufacturing process for the production of bispecific antibody products, which comprise at least two binding domains. The process comprises at least the steps of (i) providing in a perfusion bioreactor at least one mammalian cell culture, which is capable of expressing the bispecific antibody product, (ii) growing the mammalian cell culture at a first perfusion rate until a set point viable cell density is reached, and (iii) maintaining perfusion culture at a second perfusion rate, wherein the bispecific antibody product concentration in the bioreactor is kept below a threshold value. The bispecific antibody product is then subject to subsequent downstream processing. Moreover, the invention provides a bispecific antibody product produced by the continuous upstream manufacturing process.

The present application belongs to the technical field of back-contact cells, and specifically relates to a back-contact cell with a specific front-side passivation structure, and a preparation method therefor and the application thereof. The back-contact cell comprises a silicon wafer having a front side and a back side, a first semiconductor layer and a second semiconductor layer which are both disposed on the back side of the silicon wafer, and a third semiconductor layer and an antireflective layer which are sequentially disposed outwardly on the front side of the silicon wafer, wherein the third semiconductor layer includes a first intrinsic hydrogenated amorphous silicon layer and an intrinsic oxygen-doped microcrystalline silicon layer which are sequentially disposed outwardly, and the antireflective layer includes a phosphorus-doped silicon nitride layer, a non-phosphorus-doped silicon nitride layer and an oxide layer which are sequentially disposed outwardly. The back-contact cell of the present application can reduce investment in a plate-type PECVD chamber while ensuring a front-side passivation effect, such that investment in devices is reduced, the process flow is simplified, and both the cell density and cell conversion efficiency are improved while the manufacturing costs are reduced.

The invention relates to a method for directionally improving the tolerance of haematococcus pluvialis acetate, aiming at the problem that haematococcus pluvialis is sensitive to high-concentration acetate, the method comprises the following steps: gradually improving the tolerance of algae species through a staged adaptive evolution strategy, gradually adapting WT to 2.5 g / L sodium acetate through short-period-multi-cycle domestication, and obtaining a domesticated algae species HpAC25; on the basis of HpAC25, domesticating the HpAC25 to 5g / L to obtain HpAC50; further, on the basis of HpAC50, domesticating to obtain 7.5 g / L of sodium acetate, so as to obtain HpAC75. In the domestication process, suspended green motile cells are enriched through centrifugation, circulation passage is carried out every 3 days, the cell density is monitored, and the accumulative culture period reaches 189 days. Experiments show that the domesticated algae species HpAC50 and HpAC75 are cultured for 10 days under 5g / L sodium acetate and 7.5 g / L sodium acetate, and the survival rates of the domesticated algae species HpAC50 and HpAC75 reach 81.22% and 43.51% respectively and are increased by 422% and 460% compared with WT. The method is simple and convenient to operate, the tolerance of haematococcus pluvialis to acetate is remarkably enhanced, and a technical basis is provided for efficient production of astaxanthin and large-scale culture.

An object of the present invention is to provide a method in which the amount of mannose 5 in a product can be reduced in the production method for the product by cell culture, and a product that is produced by the method. According to the present invention, there is provided a production method for a product, including culturing cells in a culture container, in which the culturing is a perfusion culture method, Expressions (1) to (5) are satisfied in a production period of a product after reaching a target viable cell density, a variation of VCD during the production period is ±20% or less with respect to an average viable cell density during the production period, and a variation of VCV during the production period is ±20% or less with respect to an average value of the VCV during the production period. 0≤Gln−C≤2.5 0.5≤Gluc−C≤8.0 1.0≤NH4−C≤8.0 50≤VCD≤300 0.6×105≤VCV≤4.0×105 The meanings of the symbols in the expressions are as defined in the present specification.

The utility model discloses a cell bioreactor, belongs to the technical field of cell reaction, and aims to solve the problems that when a reaction tube is shaken manually, a culture solution and cells cannot be fully mixed and reacted, the density of added cells is low, and when the reaction tube is shaken for a long time, the arm of an operator is prone to ache. Side frames are fixed to the two sides of the top face of the bottom plate respectively, limiting grooves are formed in the tops of the inner side walls of the side frames, a tooth-shaped ring is rotationally connected into the limiting groove in one side, and a limiting ring is rotationally connected into the limiting groove in the other side. A culture solution and cells can be further mixed through the first stirring rod, and the culture solution and the cells entering the two sides of the mixing pipe can be mixed through the second stirring rod, so that the cells and the culture solution are fully contacted, mixed and reacted, and the density of the added cells is improved; the condition that the arm of an operator is prone to ache when the reaction tube is manually shaken for a long time is avoided.

Provided are methods for the creation of ultra-high density cryopreserved cell banks. In certain embodiments, these methods employ altered perfusion culture techniques that allow for production of ultra-high density cell cultures that can be cryopreserved at unexpectedly high cell densities without the need for any cell concentration steps, while retaining excellent cell viability and quality.

The invention relates to the technical field of electromagnetic compatibility (EMC) design of electronic circuits, in particular to a method suitable for a flow cytometer and capable of reducing interference of clock signals on EMC. The method comprises the steps that S100, clock circuit components are concentrated in a 5mm * 5mm area of the top layer of a PCB, routing is limited to a second layer through buried holes, and fundamental frequency is dynamically set; s200, laying copper in a key area for grounding, and adding a ground bounce suppression array to three layers; s300, arranging an upper ground copper layer and a lower ground copper layer by using a flexible circuit board; s400, covering the top circuit with a ferrite alloy cover, and welding and grounding; and S500, the flow velocity and the cell density are collected in real time, the clock frequency is dynamically adjusted, a frequency stabilization mechanism exists in an extreme scene, radiation is effectively reduced, and the detection precision and stability are improved. According to the method, the EMC interference of the clock signal of the flow cytometer is reduced from multiple dimensions of source inhibition, shielding protection, connection optimization and cooperative control.