33 results about "Cell density" patented technology

Provided are methods for the creation of ultra-high density cryopreserved cell banks. In certain embodiments, these methods employ altered perfusion culture techniques that allow for production of ultra-high density cell cultures that can be cryopreserved at unexpectedly high cell densities without the need for any cell concentration steps, while retaining excellent cell viability and quality.

This invention discloses a polyetheretherketone (PEEK) / aramid fiber composite microporous material and its molding method, belonging to the field of engineering plastics processing. The invention first modifies aramid fibers by amylation and prepares carboxylated PEEK. Covalent bonding between the amino and carboxyl groups is achieved through a chemical reaction, resulting in aramid fibers coated with PEEK. These fibers are then mixed with PEEK resin, a melt flow modifier, and additives to obtain a composite material. The composite material is then subjected to biaxial thermo-stretching orientation to improve its transverse / longitudinal orientation and crystallinity. Finally, the composite microporous material is obtained through supercritical CO2 microporous foaming. Because the foaming temperature is lower than the thermo-stretching orientation temperature, the biaxial orientation structure of the composite material is well preserved, and the well-dispersed aramid fibers effectively improve the cell wall strength, inhibit cell growth and merging, significantly increase cell density, and drastically reduce cell size, thereby constructing a high-strength, heat-insulating PEEK composite microporous material.

An exemplary method to design an integrated circuit includes generating a first floorplan for an integrated circuit, the first floorplan including a plurality of first rows having a first cell height and a plurality of second rows having a second cell height. Generating the first floorplan includes organizing the first rows and the second rows in a first row pattern. The method also includes modifying the first floorplan based on a predetermined cell density of a plurality of first cells and a plurality of second cells to generate a second floorplan, the first cells having the first cell height and the second cells having the second cell height. Modifying the first floorplan includes organizing a set of first rows and a set of second rows in a second row pattern. The second row pattern has X' first rows and Y' second rows, where X' and Y' are positive integers.

This invention provides the principle and method for influencing cellular functional excitatory expression through misaligned distribution of acoustic wave frequencies, relating to the interdisciplinary field of biological signal processing. The principle and method for influencing cellular functional excitatory expression through misaligned distribution of acoustic wave frequencies includes the following steps: Step 1: Target cell pretreatment, selecting cells to be regulated, separating, purifying, and culturing to the logarithmic growth phase, adjusting the cell density to... 1×104‑1×106cells / cm2 The cells are placed in a biocompatible culture medium. The method of this invention significantly improves the specificity of regulation. Through a frequency-function specific matching mechanism, frequency groups targeting specific cellular functions are screened. Combined with a multidimensional orbital misalignment design to avoid frequency interference, each frequency precisely activates the corresponding signaling pathway. The single-function excitation expression level of cells is more than twice that of the traditional single-frequency method, and the non-specific functional excitation rate is reduced by more than 80%, while the efficiency of action is greatly improved.

This invention relates to the field of wire and cable technology, specifically disclosing a composite sheath for cable that can be threaded through and split. The composite sheath, from the inside out, consists of a closed-cell foamed buffer layer, a flexible metal shielding layer, and a low-smoke halogen-free outer protective layer. It has an axially pre-compressed easy-tear cutting line (0.5-1mm wide, half the sheath wall thickness). The shielding layer, foaming layer, and outer protective layer are bonded together via heat sealing / co-extrusion, with an interlayer peel strength ≥2.0N / mm, and each layer is indestructible. The composite sheath length is customizable, with a maximum custom length of 3000 meters. The closed-cell foamed buffer layer has a cell density of 50-300 cells / cm³. 3 This invention combines shock absorption, heat insulation, and waterproofing functions. The flexible metal shielding layer (shielding attenuation ≥60dB) is interference-resistant and firmly bonded, while the low-smoke, halogen-free outer protective layer (oxygen index ≥35%) uses materials adapted to different scenarios. This invention solves the problems of existing composite sheaths being non-removable, easily peeling between layers, limited length, and single function. It is suitable for long-distance, multi-scenario cable protection, significantly extending cable lifespan.

This invention discloses a recombinant *E. coli* strain for producing phenyllactic acid, a lactate dehydrogenase mutant, a whole-cell catalytic method for preparing phenyllactic acid, and the application of the aforementioned recombinant *E. coli* strain or lactate dehydrogenase mutant in the catalytic preparation of phenylpyruvic acid from phenyllactic acid. This invention significantly improves catalytic efficiency by modifying the 52nd amino acid of lactate dehydrogenase through site-directed mutagenesis. After mutating glutamine (Q) to valine (V) at position 52 of the *Lactobacillus mucosa* lactate dehydrogenase, the catalytic efficiency is significantly improved even at low cell density (OD). 600 =15) and substrate 15 g / L conditions, almost complete conversion was achieved; and combined with high-density catalysis and surface display optimization, the yield of phenyl lactic acid was increased while the downstream separation and purification costs were significantly reduced, greatly enhancing the potential for industrial production.

The application provides a light material and application thereof in shoe materials, which comprises a base resin, a dispersed phase resin and an interfacial nucleating agent; the base resin is polyether amide block copolymer PEBA; the dispersed phase resin is thermoplastic polyurethane TPU; the interfacial nucleating agent is a layered silicate, and the layered silicate is dispersed at the phase interface of the PEBA and the TPU; and the solubility parameter difference of the PEBA and the TPU is large. The application constructs a "phase interface-nanoparticle" synergistic constraint system, ingeniously utilizes the specific solubility parameter difference between the PEBA and the TPU, regulates the compatibility of the two phases, and makes the two phases form an island structure with moderate interface bonding force; the layered silicate as the interfacial nucleating agent is enriched and anchored at the phase interface of the PEBA and the TPU under a specific process, the nanosheet layer at the interface acts as a heterogeneous nucleation point, greatly reduces the nucleation energy barrier, and increases the cell density.

This invention provides a Dunaliella salina mutant strain DS-HC01, which exhibits both rapid growth and high carotenoid accumulation capabilities, with the preservation number CCTCC NO: M 2026726. The mutant strain was obtained through high-energy proton beam mutagenesis at 300 MeV and a dose of 100 Gy, followed by three-stage screening. Under 25°C culture conditions, DS-HC01 overcomes the trade-off between growth and product accumulation, achieving a cell density more than 1.2 times that of the wild type on day 10, and a total carotenoid content more than 1.25 times higher. It also exhibits higher SOD activity and lower MDA content, demonstrating extremely strong antioxidant regulation capabilities and synergistic advantages in growth and pigment accumulation, making it suitable for efficient fermentation production of carotenoids.

A turbidity sensor for detecting clouding of a fluid by particulates contained in the fluid, wherein the turbidity sensor includes a fluid detection region for the reception and metrological detection of fluid; a radiation source for emitting radiation into the fluid detection region; a first radiation sensor for detecting a first part of the radiation emitted by the radiation source; and a second radiation sensor, different from the first radiation sensor, for detecting a second part of the radiation emitted by the radiation source that is different from the first part, wherein at least a portion of the fluid detection region is located between the first radiation sensor and the second radiation sensor, the second radiation sensor is located in a beam path of the first part of the radiation emitted by the radiation source that is detected by the first radiation sensor.

This invention provides a traditional Chinese medicine composition for treating anxiety, depression, and / or sleep disorders, its preparation method, and its application, belonging to the field of traditional Chinese medicine technology. The traditional Chinese medicine composition includes the following raw materials: *Aconitum carmichaelii* root slices, calcined magnetite, stir-fried jujube seed, prepared polygala tenuifolia root, prepared pinellia tuber, tangerine peel, stir-fried immature bitter orange, ginger-processed bamboo shavings, albizia bark, poria cocos, coix seed, bamboo leaf bupleurum root, oyster shell, licorice root, and dragon bone. The preparation method includes: first decocting the *Aconitum carmichaelii* root slices, then adding the remaining medicinal ingredients and decocting with water; concentrating the filtrate into a clear paste, adding excipients, and compressing into tablets. Animal experiments show that, compared with the model group, the traditional Chinese medicine composition of this invention can significantly increase the number of times mice enter the central region and the dwell time in the open field test, and improve the number of times mice enter the open arms of the elevated cruciate maze and the dwell time; hippocampal Nielsen staining shows that neurons are neatly arranged and cell density is well restored. This invention has the effects of warming the kidney and suppressing yang, resolving phlegm and calming the mind, and can be used to prepare drugs for treating anxiety, depression, and / or sleep disorders.

The application discloses a cell migration behavior prediction method based on physical information width learning, which utilizes cell scratch experiment data to construct a base function matrix, combines a Fisher-KPP reaction diffusion equation to construct a nonlinear least square problem containing physical mechanism constraints, and adopts an enhanced nonlinear least square disturbance algorithm to iteratively solve output weights, so as to realize prediction of cell density space-time evolution. The application converts traditional deep network iterative training into a nonlinear least square problem, greatly reduces a search space through physical information initialization and linearization approximation, and realizes order-of-magnitude improvement of training speed. The application constructs a width learning architecture containing feature nodes and enhanced nodes, effectively avoids the gradient vanishing problem in deep learning, combines analytical derivative calculation, eliminates cumulative error of automatic differentiation on high-order derivatives, and can more accurately capture space-time evolution characteristics in cell migration.

An optical cell density sensor is provided. The optical cell density sensor comprising: a light source arranged to emit light in a first direction for passing through a flow of fluid with suspended cells; a first detector substantially aligned with the first direction, arranged to detect attenuated light from the light source and generate an attenuated light signal; a second detector substantially offset from the first direction, arranged to detect scattered light from the light source and generate a scattered light signal; and a processor configured to calculate cell density based on the attenuated light signal and the scattered light signal.

This disclosure provides a chip layout optimization method, apparatus, device, medium, and product, relating to the fields of artificial intelligence and digital integrated circuit design technology, particularly chip layout design technology. The specific implementation scheme involves: extracting chip layout state features from a layout database; the layout state features include at least one of cell density, line length, congestion rate, timing margin, and power grid coverage; during the chip layout optimization process, combining a performance index prediction model, iteratively optimizing the layout control parameters based on the layout state features to obtain target layout control parameters; wherein, the performance index prediction model is a linear regression model. The above technical solution enables rapid prediction and dynamic optimization of layout quality.

The application discloses a fluorine-containing chain extender, a composition for preparing a PLA foaming material, the PLA foaming material and a preparation method thereof. The fluorine-containing chain extender is prepared by a solution polymerization method, and the preparation monomers include methyl methacrylate, glycidyl methacrylate and dodecafluoroheptyl acrylate, and the molar ratio is 3-6:0.1-2:0.1-2. The composition for preparing the PLA foaming material includes pure PLA and the fluorine-containing chain extender, wherein the mass of the fluorine-containing chain extender is 4-8 wt% of the mass of the pure PLA. The PLA foaming material is prepared from the composition for preparing the PLA foaming material. The PLA foaming material prepared by the application has high expansion ratio and cell density.

This invention relates to the field of fermentation technology, specifically disclosing a bacterial culture medium for high-density fermentation processes. Its purpose is to solve the problems of nutrient imbalance, byproduct inhibition, and insufficient dissolved oxygen in existing culture media during high-density bacterial fermentation, leading to low cell density and poor fermentation efficiency. The culture medium, by weight, comprises 15-30 parts carbon source, 8-18 parts nitrogen source, 0.5-2.5 parts complex trace elements, 0.3-1.2 parts growth promoter, and 0.1-0.8 parts inhibitor scavenger, diluted to 1000 parts with distilled water, with the pH adjusted to 6.5-7.8, and sterilized at 115-121℃ for 12-18 minutes before use. This invention achieves precise nutrient supply through the scientific ratio of each component, effectively removes inhibitory substances during fermentation, improves dissolved oxygen transfer efficiency, alleviates osmotic pressure stress, increases bacterial cell density by more than 30% compared to conventional culture media, shortens the fermentation cycle by 15%-25%, reduces production costs, and is suitable for high-density fermentation of various bacteria such as Escherichia coli, Bacillus, and Lactobacillus. It is applicable to industrial production scenarios such as biopharmaceuticals, food fermentation, and environmentally friendly preparations.

This invention belongs to the field of trastuzumab technology, specifically relating to a Nod2 gene knockout CHO cell line, its preparation method, and its applications. The Nod2 gene in the knockout CHO cells is lost. The CHO cells stably express trastuzumab before Nod2 gene knockout. At the same cell density, the CHO cell line shows a 1.22-1.55-fold increase in trastuzumab expression. This invention knocks out the Nod2 gene in CHO cells using a CRISPR / Cas9 system, and also provides a method for constructing the cell line, a method for producing trastuzumab using the cell line, and applications of the cell line. The Nod2 gene knockout cell line significantly increases trastuzumab expression in suspension culture while effectively reducing the aggregation level of the target protein. Furthermore, the high-yield trastuzumab trait is stably inherited and does not significantly inhibit normal cell growth, providing a new stable platform for efficient trastuzumab production and possessing significant industrial application value.

The application discloses a TteUPO mutant and application thereof, and belongs to the technical field of bioengineering and enzyme engineering.The TteUPO mutant disclosed by the application connects the nucleotide sequence of a short-chain non-specific peroxidase with a SUMO and 8HIS tag to a pET28a vector, obtains a recombinant vector, transforms E.coli BL21 (DE3), and obtains a genetically engineered bacterium; and the mutant is obtained through unit point mutation.The application uses an E.coli autoinduction system, can make shake flask culture grow to high density in a short time, contains rich nutrients in the culture medium, and does not need to monitor cell density and add IPTG in the expression process.Meanwhile, a hematin precursor 5ALA is added in the E.coli autoinduction culture medium, and finally, a high-activity short-chain non-specific peroxidase is obtained, and the enzyme after purification is reacted with anisole under the condition of hydrogen peroxide flow addition to obtain a product.

This invention relates to the field of medical image processing technology, specifically to a method and system for processing immune scoring data based on deep learning. The method includes: acquiring an immunohistochemically stained whole-section image to be scored; dividing the whole-section image into non-overlapping image blocks; performing multi-level spectral separation and decomposition on each image block to construct a spatial density distribution map of immune cells in the whole-section; segmenting the tumor central region and invasion edge region on the spatial density distribution map according to tissue morphology gradients, and extracting the corresponding local density distribution. This invention, by inverting the effective chemotaxis coefficient and effective proliferation coefficient of the reaction-diffusion coupling system from the spatial density distribution map of immune cells, can directly quantify the degree of mechanistic deviation of immune cell chemotaxis and proliferation capacity in the tumor microenvironment, overcoming the limitations of traditional methods that rely solely on static cell density counting, and giving the scoring a clear biophysical meaning.

The application is suitable for the field of biological medicine technology, and provides a mesenchymal stem cell engineering preparation loaded with drugs, a preparation method and application, and comprises the following steps: cell pretreatment, MSCs sedimentation is obtained; methylprednisolone sodium succinate is weighed, filtered and sterilized, diluted to a working concentration, and placed on ice for standby; the MSCs sediment is resuspended, the cell density is adjusted to 2x10 6 cells / mL, the cell suspension and pre-cooled MPSS working solution are continuously mixed at a volume ratio of 1:1, so that the final concentration of MPSS is 4ug / uL, the loading process lasts for 24 hours±5 minutes, and the environmental temperature is maintained at 4℃±0.5℃; post-processing and preparation forming. In the application, MSCs are used as a drug delivery carrier to realize the lesion-targeted accumulation and local release of hormones and other drugs, so that the anti-inflammatory and anti-fibrosis effects are enhanced, the toxic and side effects caused by systemic medication are significantly reduced, and finally the synergistic treatment effect of "1+1>2" is achieved.

The application discloses an expression system suitable for short-chain non-specific peroxidase and application thereof, and belongs to the technical field of enzyme engineering and genetic engineering. The expression system disclosed by the application connects the nucleotide sequence of short-chain non-specific peroxidase with a SUMO and 8HIS label to a pET28a vector to obtain a recombinant vector, and the recombinant vector is used to transform Escherichia coli BL21 (DE3) to obtain a genetically engineered bacterium; the genetically engineered bacterium is cultured in a ZYM5052 liquid culture medium by using a two-stage temperature control method, and 5ALA is added to the culture medium. The application uses an Escherichia coli self-induction system, can make shake flask culture grow to a high density in a short time, and the culture medium contains rich nutrients, and the cell density does not need to be monitored and IPTG does not need to be added during the expression process. In addition, a hemin precursor 5ALA is added to the Escherichia coli self-induction culture medium, and finally, short-chain non-specific peroxidase with high activity is obtained.

This invention discloses a numerical simulation method and apparatus for Microcystis blooms coupled with multiple hydrodynamic effects, comprising the following steps: S1, collecting basic data required for constructing a Microcystis bloom model; S2, establishing a fluid dynamics model based on a preset turbulence model, and solving the fluid dynamics model based on preset boundary conditions; S3, constructing a Microcystis dynamics model based on the response relationship between hydrodynamic elements and Microcystis population migration, buoyancy transport, growth, death, population size, and cell density, with governing equations including convection-diffusion equations, Microcystis vertical migration equations, Microcystis growth rate equations, Microcystis death rate equations, population diameter dynamic equations, and cell density dynamic equations; S4, coupling the fluid dynamics model and the Microcystis dynamics model, and obtaining the simulated concentration distribution results of Microcystis through parameter calibration and verification; This invention can fully reflect the key role of hydrodynamic elements in the bloom process, providing reliable support for bloom prediction and forecasting.

This invention discloses a method and apparatus for identifying algal blooms based on the vertical distribution and biomass of algae, comprising: collecting basic data; constructing an algal bloom model coupled with the buoyancy characteristics of Microcystis aeruginosa, wherein the algal bloom model is associated with a hydrodynamic model to simulate the vertical distribution and biomass of Microcystis aeruginosa; setting initial conditions, boundary conditions, and model parameters, dividing the vertical stratification according to water depth, covering the water surface to the bottom, and running the model to obtain Microcystis aeruginosa cell density data at each time point and at each depth layer; calculating the average vertical biomass of Microcystis aeruginosa and vertical distribution indicators based on the Microcystis aeruginosa cell density data at each depth layer and determining the aggregation state; and determining whether an algal bloom has occurred and the risk of an outbreak based on the average vertical biomass and vertical aggregation state of Microcystis aeruginosa. This invention uses a dual-dimensional identification of vertical aggregation state and biomass, which makes up for the shortcomings of single biomass identification, and can more accurately predict the risk of algal bloom outbreaks and improve the effectiveness of prevention and control.

The application discloses flower-shaped silver micro-nanoparticles and application of the flower-shaped silver micro-nanoparticles in preparation of polymer microporous materials, and the flower-shaped silver micro-nanoparticles are prepared by the following steps: reacting silver nitrate with a structure directing agent in a solvent, adding a reducing agent into the solution, continuing to react, centrifuging, washing and drying the precipitate to obtain flower-shaped silver powder; adding all the flower-shaped silver powder into a reaction kettle, controlling the humidity of nitrogen atmosphere in the reaction kettle to be 10%-15% and keeping for 30 min, closing the air knob, rapidly injecting a modifier, sealing the reaction kettle, and reacting for 30 min at room temperature to obtain the flower-shaped silver micro-nanoparticles; the flower-shaped silver micro-nanoparticles prepared by the application can be used as a high-efficiency heterogeneous nucleating agent, can significantly improve nucleation efficiency, can reduce cell size, and can make cell density reach 10-11 orders of magnitude and be improved by 1-2 orders of magnitude. The method is simple in operation and easy to control, and is expected to be used in industrialized application of polymer supercritical CO2 foaming and improve economic benefits.

This invention belongs to the field of CHO cell technology, specifically relating to a genetically modified CHO cell line for trastuzumab, its preparation method, and its applications. The genetically modified CHO cell line for trastuzumab is obtained by deleting the Ddit4 gene from the original CHO cell line. At the same cell density, the genetically modified CHO cell line shows a 1.62-2.17-fold increase in trastuzumab expression compared to the original CHO cell line. This invention specifically enhances the protein synthesis capacity of CHO cell lines by knocking out the Ddit4 gene, providing a new and stable platform for the efficient production of trastuzumab.

Provided herein are compositions and methods for engineering a bacterium comprising a reduced capacity for cellulose formation, a reduced capacity for foaming behavior, or an increased capacity for cell density for enhancing bioprocessing outcomes associated with the fermentative production of industrially relevant bioproducts.

This application discloses a metal-oxide-semiconductor field-effect transistor (MOSFET), whose cell includes a substrate, an epitaxial layer on the substrate, a drift region extending from a first surface of the epitaxial layer into the epitaxial layer, a body region extending from the surface of the drift region into the drift region, a groove extending downward from the upper surface of the middle of the body region, a source region extending from the lower surface of the groove into the body region, and a body contact region extending from the lower surface of the groove to the bottom of the body region. The body contact region contacts the source region and is located in the middle region of the body region, while the source regions are located on both sides of the body contact region. This MOSFET, by setting a groove structure, reduces the horizontal length of the channel, lowers the cell size, thereby increasing the cell density and on-resistance, and enhancing the reverse breakdown voltage.

The application provides a preparation method of an ADC foaming agent for improving foaming cell density, which comprises the following steps: step 1: mixing a modified inorganic nucleating agent and water according to a predetermined mass ratio, adding a dispersing agent, uniformly mixing, and preparing a uniform and stable suspension slurry to prevent the nucleating agent from agglomerating; step 2: when the conversion rate of biurea reaches 80%-85%, the suspension slurry is added, and the inorganic nucleating agent is uniformly attached to the surface of the ADC crystal; step 3: continuing the oxidation reaction until the conversion rate of biurea reaches more than 92%, and then performing washing, centrifugation and drying to obtain the ADC foaming agent. The ADC foaming agent for improving foaming cell density is prepared by the above method. In the preparation process of the ADC foaming agent, the inorganic nucleating agent is added, compared with adding the inorganic nucleating agent in the subsequent preparation process of the foaming material, and the foaming cell density can be significantly improved.

This invention provides a method for isolating and identifying interstitial cells from Bactrian camels, belonging to the field of cell isolation technology. A mixture of 0.08–0.12% collagenase IV solution and 0.23–0.27% trypsin-EDTA at a volume ratio of 1:2 is used to digest Bactrian camel testicular tissue blocks. The mixture is then purified by hypotonic treatment to remove excess germ cells, differential adhesion purification to remove a large number of supporting cells, and Percoll density gradient centrifugation to remove a small number of supporting cells. The suspension, obtained from a 34%–60% Percoll gradient, is transferred to a new culture flask to obtain interstitial cells with a purity ≥95%. Identification is performed using morphological observation, immunofluorescence staining, and secretory function testing. The interstitial cells isolated from Bactrian camels using this invention can be used for research on the reproductive performance of Bactrian camels, laying the foundation for understanding the reproductive performance of Bactrian camels.

We provide bioprocessing systems for cell immunotherapy that reduce contamination risks by increasing automation and minimizing human handling. We offer bioprocessing systems for cell therapy drug manufacturing that balance the need for development flexibility with the consistency of mass production, and meet the diverse needs of customers who require different processes. [Solution] A bioprocessing method for cell therapy comprises the steps of: genetically modifying a population of cells in a bioreactor vessel to produce a population of genetically modified cells; and amplifying the population of genetically modified cells in the bioreactor vessel to produce a sufficient number of genetically modified cells for use in cell therapy treatment without removing the population of genetically modified cells from the bioreactor vessel. The cells are immobilized on a gas-permeable, liquid-impermeable membrane for amplification and are resuspended when or after reaching a desired cell density.