260 results about "Cell density" patented technology

The present invention provides a serum-free culture medium for in vitro suspension culture of porcine muscle stem cells and its application, which can help porcine muscle stem cells expand and be suspended under serum-free and carrier-free conditions. Compared with traditional porcine muscle stem cell culture protocols and culture media, this serum-free suspension culture medium enables porcine muscle stem cells to be cultured continuously at high densities in both batch and fed-batch culture processes under serum-free and carrier-free suspension culture conditions, while maintaining high cell viability and achieving a peak cell density of 1×10 7 The beneficial effect of the present invention is to effectively solve the problem of serum-free and carrier-free efficient suspension expansion of cell cultured meat seed cells, and provide a technical and raw material basis for the industrial production of cell cultured meat.

The invention provides a serum-free culture medium for in-vitro suspension culture of porcine muscle stem cells and application of the serum-free culture medium. The serum-free culture medium can help the porcine muscle stem cells to be subjected to suspension enlarged culture under serum-free and carrier-free conditions. Compared with a traditional porcine muscle stem cell culture scheme and culture medium, the serum-free suspension culture medium has the advantages that high-density continuous culture of porcine muscle stem cells can be realized in a batch culture process and a fed-batch culture process under the serum-free carrier-free suspension culture condition, high cell viability is maintained, and the survival rate of the porcine muscle stem cells is increased. The peak density of the cells reaches 1 * 10 < 7 > cells / mL or above, and the cell viability is maintained to be 90% or above. The method has the beneficial effects that the problem of serum-free carrier-free efficient suspension amplification of the seed cells of the cell culture meat is effectively solved, and a technical and raw material basis is provided for industrial production of the cell culture meat.

According to the microscopic cell image tracking method and device based on the graph neural network, the electronic equipment and the storage medium provided by the invention, a training set cell image is acquired and a training label is marked, a cell segmentation model is trained according to the training set cell image, and other data sets are predicted according to the cell segmentation model; according to the tracking method provided by the invention, the dependence on manual design can be reduced, the feature expression ability can be improved, the relationship between the cells can be modeled through the graph structure, the tracking precision under the condition of dense and shielded cells can be improved, and the tracking accuracy can be improved. Meanwhile, in combination with time sequence data, the time continuity of cell movement is captured, and the tracking stability is improved.

The invention provides a preparation method of an ADC foaming agent for improving foaming pore density, which comprises the following steps: step 1, mixing a modified inorganic nucleating agent with water according to a predetermined mass ratio, adding a dispersing agent, and uniformly mixing to prepare uniform and stable suspension slurry to prevent agglomeration of the nucleating agent; 2, when the conversion rate of the biurea reaches 80%-85%, adding the suspension slurry, so that the inorganic nucleating agent is uniformly attached to the surface of the ADC crystal; and 3, continuing the oxidation reaction until the biurea conversion rate reaches 92% or above, and then washing, centrifuging and drying to obtain the ADC foaming agent. The ADC foaming agent for improving the foaming pore density is prepared by the method. The inorganic nucleating agent is added in the stage of preparing the ADC foaming agent, and compared with the inorganic nucleating agent added in the subsequent foam material preparation process, the density of foaming pores can be remarkably improved.

A semiconductor device includes a gate structure including conductive layers and insulating layers alternately stacked with each other, channel structures passing through the gate structure and arranged in a first direction, a cutting structure extending in the first direction and passing through the channel structures, and a first slit structure passing through the gate structure and extending in a second direction crossing the first direction.

The invention discloses an expression system suitable for short-chain non-specific peroxidase and application of the expression system, and belongs to the technical field of enzyme engineering and genetic engineering. According to the expression system disclosed by the invention, a nucleotide sequence of short-chain non-specific peroxidase with SUMO and 8HIS tags is connected to a pET28a vector to obtain a recombinant vector, and escherichia coli BL21 (DE3) is transformed to obtain a genetically engineered bacterium; genetically engineered bacteria are subjected to two-stage temperature control culture in a ZYM5052 liquid culture medium, and meanwhile, 5ALA is added into the culture medium. According to the invention, an escherichia coli self-induction system is used, so that a shake-flask culture can grow at high density in a short time, the culture medium contains rich nutrient substances, and monitoring of cell density and adding of IPTG are not needed in the expression process. Meanwhile, a heme precursor 5ALA is added into an escherichia coli self-induction culture medium, and finally the high-activity short-chain non-specific peroxidase is obtained.

Described herein are methods, systems, and programming for determining a tumor immunophenotype of an image of a tumor. Some embodiments include dividing an image into tiles depicting tumor epithelium and / or tumor stroma. For each tile, an epithelium-immune cell density and a stroma-immune cell density may be calculated based on a number of immune cells identified in the tumor epithelium and the tumor stroma, respectively. Based on the epithelium-immune cell density and the stroma-immune cell density, an inflammation type of the type may be determined, and a tumor immunophenotype may be determined based on each tile's inflammation type.

The invention provides a method for promoting synthesis of microcystis astaxanthin by adjusting the concentration of a stress substance, which comprises the following steps of: firstly, culturing microcystis algal bloom or microcystis aeruginosa to a logarithmic phase by adopting a BG11 culture medium under the conditions of specific temperature, illumination and shaking table rotating speed, and adjusting the cell density by centrifuging and resuspending; then, NaCl, KCl, beta-violet ketone, longifolene and H2O2 with different concentrations are added into the algae liquid respectively, control is set, through multi-dimensional index measurement, the algae cell density, the ROS level, the photosynthetic pigment content, the photosynthetic performance and the level of astaxanthin and a precursor thereof are monitored when treatment is carried out for 0 day, 2 days, 4 days and 6 days, the expression quantity of astaxanthin biosynthesis related genes is further analyzed, and the astaxanthin biosynthesis related genes are obtained. Therefore, the optimal concentration of NaCl, KCl, beta-violet ketone, longifolene and H2O2 is determined, the effect of promoting astaxanthin synthesis is compared, and a theoretical basis and a precise regulation and control method are provided for industrial production of astaxanthin by using microcystis.

The present invention relates to a labyrinth seal for a turbine engine, in particular of an aircraft, comprising a rotor element and a stator element extending around the rotor element, the rotor element being suitable for rotating relative to the stator element about an axis of rotation having an axial direction (DA), the rotor element comprising an annular lip having an outer radial end extending towards an abradable element (57) carried by the stator element, the outer radial end of the annular lip having a corrugation in the axial direction (DA) and a non-zero axial expanse (E5) associated with the corrugation, the abradable element (57) comprising a plurality of cells (50a, 50b) arranged adjacent to one another along the axial direction (DA) and an ortho-radial direction (O), the cells (50a, 50b) comprising walls which extend in an essentially radial direction, the cells being distributed with a first cell density in a first densified annular zone (Z51) of the abradable element, said densified annular zone (Z51) being located opposite the radial end of the lip, said densified annular zone having an axial expanse less than or equal to the axial expanse of the outer radial end of the lip, the cells being distributed according to a reference density of cells outside said first zone, the first density being greater than the reference density.

The invention discloses a physiological microenvironment-based cell culture simulation system, which relates to the field of cell culture, and comprises a main control unit adopting an embedded microcontroller; the multi-mode environment simulation unit is connected with the main control unit and comprises a fluid shear force module and a mechanical stretching module; the multi-parameter environment monitoring unit is connected with the main control unit and comprises an optical sensor for monitoring cell density, a pH sensor for monitoring environment parameters and a temperature sensor; the culture chamber is used for accommodating a cell culture; the environment regulation and control unit is used for regulating culture environment parameters; the multi-modal environment simulation unit and the multi-parameter environment monitoring unit are integrated, and the main control unit optimizes a multi-target control strategy in a manner of dynamically adjusting a weight coefficient based on cell growth state parameters monitored in real time, so that accurate and adaptive closed-loop management of cell culture is realized.

The present application belongs to the technical field of back-contact cells, and specifically relates to a back-contact cell with a specific front-side passivation structure, and a preparation method therefor and the application thereof. The back-contact cell comprises a silicon wafer having a front side and a back side, a first semiconductor layer and a second semiconductor layer which are both disposed on the back side of the silicon wafer, and a third semiconductor layer and an antireflective layer which are sequentially disposed outwardly on the front side of the silicon wafer, wherein the third semiconductor layer includes a first intrinsic hydrogenated amorphous silicon layer and an intrinsic oxygen-doped microcrystalline silicon layer which are sequentially disposed outwardly, and the antireflective layer includes a phosphorus-doped silicon nitride layer, a non-phosphorus-doped silicon nitride layer and an oxide layer which are sequentially disposed outwardly. The back-contact cell of the present application can reduce investment in a plate-type PECVD chamber while ensuring a front-side passivation effect, such that investment in devices is reduced, the process flow is simplified, and both the cell density and cell conversion efficiency are improved while the manufacturing costs are reduced.

The invention relates to a method for directionally improving the tolerance of haematococcus pluvialis acetate, aiming at the problem that haematococcus pluvialis is sensitive to high-concentration acetate, the method comprises the following steps: gradually improving the tolerance of algae species through a staged adaptive evolution strategy, gradually adapting WT to 2.5 g / L sodium acetate through short-period-multi-cycle domestication, and obtaining a domesticated algae species HpAC25; on the basis of HpAC25, domesticating the HpAC25 to 5g / L to obtain HpAC50; further, on the basis of HpAC50, domesticating to obtain 7.5 g / L of sodium acetate, so as to obtain HpAC75. In the domestication process, suspended green motile cells are enriched through centrifugation, circulation passage is carried out every 3 days, the cell density is monitored, and the accumulative culture period reaches 189 days. Experiments show that the domesticated algae species HpAC50 and HpAC75 are cultured for 10 days under 5g / L sodium acetate and 7.5 g / L sodium acetate, and the survival rates of the domesticated algae species HpAC50 and HpAC75 reach 81.22% and 43.51% respectively and are increased by 422% and 460% compared with WT. The method is simple and convenient to operate, the tolerance of haematococcus pluvialis to acetate is remarkably enhanced, and a technical basis is provided for efficient production of astaxanthin and large-scale culture.

Provided are methods for the creation of ultra-high density cryopreserved cell banks. In certain embodiments, these methods employ altered perfusion culture techniques that allow for production of ultra-high density cell cultures that can be cryopreserved at unexpectedly high cell densities without the need for any cell concentration steps, while retaining excellent cell viability and quality.

The invention relates to the technical field of electromagnetic compatibility (EMC) design of electronic circuits, in particular to a method suitable for a flow cytometer and capable of reducing interference of clock signals on EMC. The method comprises the steps that S100, clock circuit components are concentrated in a 5mm * 5mm area of the top layer of a PCB, routing is limited to a second layer through buried holes, and fundamental frequency is dynamically set; s200, laying copper in a key area for grounding, and adding a ground bounce suppression array to three layers; s300, arranging an upper ground copper layer and a lower ground copper layer by using a flexible circuit board; s400, covering the top circuit with a ferrite alloy cover, and welding and grounding; and S500, the flow velocity and the cell density are collected in real time, the clock frequency is dynamically adjusted, a frequency stabilization mechanism exists in an extreme scene, radiation is effectively reduced, and the detection precision and stability are improved. According to the method, the EMC interference of the clock signal of the flow cytometer is reduced from multiple dimensions of source inhibition, shielding protection, connection optimization and cooperative control.

The invention provides a microfiber for loading pancreas islet, a preparation method and application, the preparation method comprises the following steps: S1, preparing a micro-fluidic chip and building a pneumatic control micro-fluidic spinning device, the micro-fluidic chip is provided with channels for conveying a core flow, a sample flow and a sheath flow, the micro-fluidic chip further comprises a PDMS chamber, and the PDMS chamber is provided with a PDMS cavity; a channel for periodically pressurizing to expand so as to squeeze and close the core flow; s2, adding collagen into the core flow, then adding beta-TC6 cells to enable the cell density of the core flow to be 4 * 10 < 6 > mL <-1 > to 8 * 10 < 6 > mL <-1 >, respectively controlling the flow rates of the core flow, the sample flow and the sheath flow to be 0.8-1.2 [mu] L / min, 18-22 [mu] L / min and 80-120 [mu] L / min, and carrying out preparation under the frequency of 0.8-1.2 Hz; according to the method, a pneumatic control droplet generation method is combined with microfluidics, so that the high-flux islet-like can be prepared more simply and quickly, and the difference between the islet-like and an in-vivo complex microenvironment is small; the prepared microfiber is continuous and complete, and a large number of water-phase liquid drops containing beta-TC6 cells are uniformly distributed in the microfiber.

The invention discloses a preparation method and application of high-activity recombinant collagen, and relates to the field of biological medicine, and the preparation method comprises the following steps: S1, providing engineering bacteria containing a recombinant collagen expression vector, and performing seed culture; s2, inoculating the seed solution into a fermentation culture medium for fermentation culture; s3, in the fermentation process, when the thallus density reaches a preset value, a signal factor without an animal source is added into a fermentation system, and the signal factor is bionic polypeptide; s4, after the signal factors are added, the fermentation conditions are adjusted, and target collagen expression is induced; according to the preparation method and the application of the high-activity recombinant collagen, an endogenous metabolic network of bacteria is actively regulated and controlled by accurately adding animal-source-free bionic polypeptide signal factors in the fermentation process of engineering bacteria, and correct folding and specific post-translational modification of target collagen are induced; therefore, the hydroxylation rate and the biological activity of the recombinant collagen are improved, and the structure and the function of the recombinant collagen are closer to those of natural collagen of a human body.

The invention relates to the technical field of biological cell culture media, and discloses a culture medium additive and a culture medium capable of improving the cell caking condition in the CHO cell culture process, and a method for culturing CHO cells in vitro by using the culture medium. According to the serum-free culture medium for culturing the CHO cells, calcium, magnesium and phosphorus substances with the original concentration and calcium, magnesium and phosphorus with the adjusted concentration are additionally added, the culture medium is optimized, the cell caking condition in the CHO cell culture process is improved, and the serum-free culture medium for culturing the CHO cells is simple in component, low in cost and free of protein and serum. When the optimized culture medium is used for culturing to the seventh day, the cell caking rate can be reduced from 37.15% to 18.38%, and the cell density can be increased from 4.56 * 10 < 6 > cells / mL to 17.4 * 10 < 6 > cells / mL.

The invention discloses a method for researching influence on hemolytic activity of marine microalgae based on a transcriptome technology, and relates to the field of hemolytic activity analys.The method comprises the steps that pretreatment is conducted on the marine microalgae based on culture requirements, and cystic morphological cell density and hemolytic activity of the marine microalgae are measured according to experimental design rules after pretreatment is completed; carrying out transcriptome sample collection operation by utilizing the marine microalgae subjected to pretreatment, constructing a library according to a transcriptome sample collection result, and obtaining a gene function annotation and a differential expression gene result; the growth conditions of the marine microalgae under different temperature conditions are analyzed according to cystic morphological cell density and hemolytic activity, and the regulatory gene influencing the hemolytic activity of the marine microalgae is obtained by combining gene function annotation and differential expression gene results. According to the method, the hemolytic activity of the marine microalgae cultured under different temperature conditions is extracted, so that the aim of researching related metabolic pathways possibly participating in toxin synthesis and hemolytic activity regulation is fulfilled.

This invention discloses a polyetheretherketone (PEEK) / aramid fiber composite microporous material and its molding method, belonging to the field of engineering plastics processing. The invention first modifies aramid fibers by amylation and prepares carboxylated PEEK. Covalent bonding between the amino and carboxyl groups is achieved through a chemical reaction, resulting in aramid fibers coated with PEEK. These fibers are then mixed with PEEK resin, a melt flow modifier, and additives to obtain a composite material. The composite material is then subjected to biaxial thermo-stretching orientation to improve its transverse / longitudinal orientation and crystallinity. Finally, the composite microporous material is obtained through supercritical CO2 microporous foaming. Because the foaming temperature is lower than the thermo-stretching orientation temperature, the biaxial orientation structure of the composite material is well preserved, and the well-dispersed aramid fibers effectively improve the cell wall strength, inhibit cell growth and merging, significantly increase cell density, and drastically reduce cell size, thereby constructing a high-strength, heat-insulating PEEK composite microporous material.

An object of the present invention is to provide a cell culture method and a production method for a product, in which an occurrence of aggregates in foam is suppressed.According to the present invention, there is provided a cell culture method of culturing cells at a cell density of 30×106 cells / mL or more and 400×106 cells / mL or less in a culture solution, the method including: step A of adjusting a poloxamer concentration in a foam liquid constituting foam to be 6 times or less a poloxamer concentration in the culture solution; or step B of adjusting a viscosity of the foam liquid constituting the foam to be 1.5 mPa·s or less under conditions of 35° C. or higher and 38° C. or lower.

An exemplary method to design an integrated circuit includes generating a first floorplan for an integrated circuit, the first floorplan including a plurality of first rows having a first cell height and a plurality of second rows having a second cell height. Generating the first floorplan includes organizing the first rows and the second rows in a first row pattern. The method also includes modifying the first floorplan based on a predetermined cell density of a plurality of first cells and a plurality of second cells to generate a second floorplan, the first cells having the first cell height and the second cells having the second cell height. Modifying the first floorplan includes organizing a set of first rows and a set of second rows in a second row pattern. The second row pattern has X' first rows and Y' second rows, where X' and Y' are positive integers.

The invention discloses a haematococcus culture dissolved oxygen coupling regulation and control method for a corrosion-resistant photoreactor, relates to the field of bioengineering, and builds a photoreactor made of a corrosion-resistant material and a matched regulation and control system; preparing a special improved BG-11 culture medium for haematococcus and injecting the culture medium into the photoreactor; oxygen transfer coefficients under different ventilation rates are calibrated through a dynamic method, a ternary equation of light intensity, cell density and oxygen production rate is fitted by combining photosynthetic characteristics of haematococcus, and a basic model for dynamic prediction of dissolved oxygen is established. According to the method, the photoreactor made of a corrosion-resistant material and a matched regulation and control system are built, so that the interference of material dissolution on the culture medium is effectively avoided, and the stability of a haematococcus culture environment is guaranteed. Based on a ternary fitting equation of an oxygen transfer coefficient calibrated by a dynamic method, light intensity, cell density and oxygen production rate, a basic model for dynamic prediction of dissolved oxygen is constructed, and accurate coupling regulation and control of dissolved oxygen are realized by combining model prediction control and reinforcement learning agents.

The invention discloses an osteoclast culture method as well as a culture medium and application thereof, the culture method is based on primary monocyte extraction, inoculation is performed according to a specific density, then quantitative M-CSF is added, and culture is performed overnight; on the second day, when the cell confluence degree of the mononuclear bone marrow hematopoietic stem cells is 5-30%, supplementing quantitative RANKL into each hole, continuously culturing for 2-4 days, then changing the solution, and obtaining mature osteoclasts on the sixth-seventh day. According to the invention, the optimization relationship among the cell activity, the cell density and the culture medium in the primary culture process of the osteoclast is deeply studied, and from the perspective of cost reduction and efficiency improvement, the use cost of cell factors is remarkably reduced, the number of times of liquid change is reduced, and the traditional culture time of 10-12 days (calculated from cell extraction) is shortened to 6-7 days. The average diameter of the mature osteoclast cultured by the method is larger, the number of fused cell nucleuses is larger, the space proportion of the mature osteoclast is not less than 80%, and the cell quality is obviously higher than that in the prior art.

The invention provides an injection for inducing mesenchymal stem cells. The induced mesenchymal stem cell injection is prepared from induced mesenchymal stem cells modified by hepatocyte growth factors with the cell density being 1.0 * 10 < 5 >-2.0 * 10 < 7 > / ml, HSA with the mass concentration being 0.25%-6%, DMSO with the mass concentration being 5%-10%, glucose with the mass concentration being larger than 0 and smaller than 1%, compound amino acid with the concentration being 0.5-5 mg / mL and the balance compound electrolyte injection. And the induced mesenchymal stem cell injection has a pH value of 6.8 to 7.5. The induced mesenchymal stem cell injection is good in homogeneity, small in batch difference and stable in curative effect, can effectively promote damaged tissue repair, can be directly intravenously injected after cryopreservation and recovery, keeps high cell viability and drug activity, achieves an excellent treatment effect, has good room-temperature stability, is convenient to transport and store, and is suitable for clinical application. The method is suitable for different clinical application occasions, and the clinical application range of the mesenchymal stem cells is expanded.

The application relates to the fields of biological medicine and computing simulation, and specifically discloses an immune cell expansion process simulation and process parameter optimization system, which collects multiple groups of candidate process parameters, introduces process disturbance, generates a dynamic trajectory set of living cell density, viability and metabolic by-products, and converts the dynamic trajectory set into a first probability distribution; calculates an entropy regularization distribution distance between the first probability distribution and a second probability distribution corresponding to ideal expansion kinetics as a matching degree measurement; constructs a difference response surface with the candidate process parameters and the distance value as samples, takes the current optimal distance as a benchmark, and calculates potential expected values of each candidate parameter by using the response surface; iteratively selects the parameter with the maximum potential expected value until the distance reduction amplitude is lower than a threshold value, and outputs the optimal process parameter; the application can avoid local optimization, improve the global optimization, and reduce the simulation rounds through dynamic distribution matching and difference response surface iterative optimization.

Disclosed is an in-process cell monitoring device comprising: a flow channel having at least one inlet and at least one outlet exposeable to a cell culture; a microscope positionable to view the contents of a region of the channel; and a computer operable at least to count any cells in the region, providing a closed fluid circuit for cell monitoring. Disclosed also is a bioreactor including a cell culture volume, and an in-process cell monitoring device, said device comprising: a flow channel having at least one inlet and at least one outlet each in fluid communication with the volume, of sufficient cross-sectional area to allow fluids to drain from the inlet to the outlet; and a microscope positionable to view the contents of a region of the channel, and also A method for monitoring a cell culture including determining cell density

This invention provides the principle and method for influencing cellular functional excitatory expression through misaligned distribution of acoustic wave frequencies, relating to the interdisciplinary field of biological signal processing. The principle and method for influencing cellular functional excitatory expression through misaligned distribution of acoustic wave frequencies includes the following steps: Step 1: Target cell pretreatment, selecting cells to be regulated, separating, purifying, and culturing to the logarithmic growth phase, adjusting the cell density to... 1×104‑1×106cells / cm2 The cells are placed in a biocompatible culture medium. The method of this invention significantly improves the specificity of regulation. Through a frequency-function specific matching mechanism, frequency groups targeting specific cellular functions are screened. Combined with a multidimensional orbital misalignment design to avoid frequency interference, each frequency precisely activates the corresponding signaling pathway. The single-function excitation expression level of cells is more than twice that of the traditional single-frequency method, and the non-specific functional excitation rate is reduced by more than 80%, while the efficiency of action is greatly improved.

The invention belongs to the field of biological medicine, and relates to a curcumin-loaded bone marrow mesenchymal stem cell source exosome which is obtained by the following steps: step 1, culturing BMSCs (bone marrow mesenchymal stem cells): simultaneously adding 10% of fetal calf serum and 1% of myclostreptomycin into an MEM culture medium, culturing at 37 DEG C under 5% CO2, and counting and subculturing when the cell density reaches 90%; 2, dissolving Cur in a DMSO solution, preparing a mother solution with the culture medium, and filtering and diluting to 30-50 [mu] g / mL; adding into BMSCs (bone marrow mesenchymal stem cells) with the cell density of 85-100%, and culturing for 12-36 hours; collecting supernate, and carrying out gradient centrifugation to obtain the mesenchymal stem cell source exosome loaded with curcumin. The invention provides a novel administration route which depends on the self function of the mesenchymal stem cell source exosome and the self function of the curcumin at the same time.

An additive manufacturing method for at least one metallic heating disk for an exhaust aftertreatment system, comprising: additively generating at least one heating disk comprising: a plurality of cells comprising a honeycomb cell structure; parallel discontinuous slots; and locally varying cell densities.

A method for large-scale expansion of natural killer (NK) cells in a cell culture medium according to the invention, comprising the steps of: adding said cells to a culture system comprising a growth medium supplemented with serum selected from the group consisting of human serum and autologous plasma; cRX 527 with an effective concentration is added; the cells are expanded and heated within the system wherein the effective concentration of CRX 527 in the growth medium is in the range of 0.01 [mu] g / ml to 10 [mu] g / ml, preferably 100 ng / ml, and the ratio between the cell density and the total volume of the growth medium is in the range of 1.5 to 2.