1083 results about "Cell density" patented technology

Disclosed are systems and methods for manipulating chemical, biological, and / or biochemical samples, optionally supported on substrates and / or within chambers, for example biological samples contained on chips, within biological chambers, etc. In certain embodiments, an apparatus configured to be able to position a chamber or other substrate in one or more modules surrounding the apparatus is disclosed. The apparatus may be configured to be able to move the chamber or substrate in any set of directions, such as radially, vertically, and / or rotationally, with respect to the apparatus. The apparatus may be manually operated and / or automatically controlled. Examples of modules include, but are not limited to, stacking or holding modules, barcode readers, filling modules, sampling modules, incubation modules, sensor modules (e.g., for determining cell density, cell viability, pH, oxygen concentration, nutrient concentration, fluorescence measurements, etc.), assay modules (e.g., for ELISA or other biological assays), data analysis and management modules, control modules, etc. Sensors, control systems, and the like may also be positioned to facilitate operation of the device. Certain embodiments of the invention may be used, for example, to promote or optimize chemical synthesis or cell or biological growth, for instance, for the production of compounds such as drugs or other therapeutics.

Described herein are environmentally isolated tissue culture devices that may be used for cell culture, as well as systems including these devices and methods for using them. These devices may include control features for regulating the micro-environment within a well or wells of the device. For example, on-board features may regulate the temperature, humidity, pH, media level, media composition, CO2 / O2 / N2 levels, drug concentration, cell density, byproduct (or product) production, and mixing of materials within the chamber. Material may be added to or withdrawn from the wells of the device without opening the device. Also described herein are controllers for analyzing and controlling the micro- environment within the well. Thus, the plates described herein may be used without requiring a separate incubator, allowing cells to be analyzed (e.g., imaged) continuously, allowing real-time reactions while monitoring under a microscope for hours, days or even weeks.

A single transistor vertical memory gain cell with long data retention times. The memory cell is formed from a silicon carbide substrate to take advantage of the higher band gap energy of silicon carbide as compared to silicon. The silicon carbide provides much lower thermally dependent leakage currents which enables significantly longer refresh intervals. In certain applications, the cell is effectively non-volatile provided appropriate gate bias is maintained. N-type source and drain regions are provided along with a pillar vertically extending from a substrate, which are both p-type doped. A floating body region is defined in the pillar which serves as the body of an access transistor as well as a body storage capacitor. The cell provides high volumetric efficiency with corresponding high cell density as well as relatively fast read times.

NRAM arrays with nanotube blocks, traces and planes, and methods of making the same are disclosed. In some embodiments, a nanotube memory array includes a nanotube fabric layer disposed in electrical communication with first and second conductor layers. A memory operation circuit including a circuit for generating and applying a select signal on first and second conductor layers to induce a change in the resistance of the nanotube fabric layer between the first and second conductor layers is provided. At least two adjacent memory cells are formed in at least two selected cross sections of the nanotube fabric and conductor layers such that each memory cell is uniquely addressable and programmable. For each cell, a change in resistance corresponds to a change in an informational state of the memory cell. Some embodiments include bit lines, word lines, and reference lines. In some embodiments, 6F2 memory cell density is achieved.

A circularizated semiconductor laser diode (CSLD), such as for example a vertical cavity surface emitting laser (VCSEL) may be used for optical measurements. The CSLD may be used in a cell density probe to perform cell density determination and / or turbidity determination, such as in a biotech, fermentation, or other optical absorbance application. The cell density probe may comprise a probe tip section made from a polytetrafluoroethylene material, which provides sealability, ease of manufacture, durability, cleanability, optical semi-transparency at visible and near infrared wavelengths, and other advantages. The probe tip advantageously provides an optical gap that allows for in situ measurements of optical measurements including but not limited to absorbance, scattering, and fluorescence.

A multiphase culturing process for high density heterotrophic microalgal growth uses crude glycerol as the primary carbon source and produces ω-3 fatty acids. The process uses multiphase growth conditions that decouple the phases of increasing cell density and increasing cell size and fatty acid production. The entire process is integrated with biodiesel production.

The present invention provides improved methods of production of viral antigen on a culture of adherent cells bound to a microcarrier, wherein the methods provide for increased viral antigen yield per culture medium volume. The invention is also directed to a cell culture biomass of adherent cells having increased cell density and microcarrier concentration compared to the respective confluent cell culture.

The invention relates to extruded foaming thermoplastic polyurethane elastomer particles and a preparation method thereof. The particles comprise the following components in parts by weight: 100 parts of thermoplastic polyurethane elastomer, 0.01-0.5 part of foaming nucleating agent, and 0.01-0.2 part of antioxidant. The preparation method comprises steps of firstly, mixing the materials, then throwing the mixture into an extruder to prill to obtain a bead raw material suitable for foaming, finally, throwing the beads into a special extruder for foaming, and after foaming by an opening die, pelletizing underwater so as to prepare the particles. The thermoplastic polyurethane foaming particles are prepared through an extrusion method; by controlling the foaming technology conditions, the foaming particles are controllable in density and have uniform cell density; the whole production method is simple to operate, has no special limitation and requirement on the equipment, and is applicable to industrial continuous production.

The invention discloses a small ball algae cultivation method with high density and high quality, which comprises the following phases: high-density heterotrophy cultivation, high-density algae liquid dilution and photo-autotroph cultivation. The invention makes the small ball algae reach high-density level within shorter cultivation period, which improves the small ball algae quality.

High cell density cultures of yeast were found to have higher tolerance for butanol in the medium. The high cell density yeast cultures had greater survival and higher glucose utilization than cultures with low cell densities. Production of butanol using yeast in high cell density cultures is thus beneficial for improving butanol production.

Neo-cartilage constructs suitable for implantation into a joint cartilage lesion in situ and a method for repair and restoration of function of injured, traumatized, aged or diseased cartilage. The construct comprises at least chondrocytes incorporated into a support matrix processed according to the algorithm comprising variable hydrostatic or atmospheric pressure or non-pressure conditions, variable rate of perfusion, variable medium composition, variable temperature, variable cell density and variable time to which the chondrocytes are subjected.

NRAM arrays with nanotube blocks, traces and planes, and methods of making the same are disclosed. In some embodiments, a nanotube memory array includes a nanotube fabric layer disposed in electrical communication with first and second conductor layers. A memory operation circuit including a circuit for generating and applying a select signal on first and second conductor layers to induce a change in the resistance of the nanotube fabric layer between the first and second conductor layers is provided. At least two adjacent memory cells are formed in at least two selected cross sections of the nanotube fabric and conductor layers such that each memory cell is uniquely addressable and programmable. For each cell, a change in resistance corresponds to a change in an informational state of the memory cell. Some embodiments include bit lines, word lines, and reference lines. In some embodiments, 6F2 memory cell density is achieved.

The invention discloses the composition of a biodegradable polylactic acid (PLA) foaming material and a preparation method thereof. The method for preparing the biodegradable polylactic acid foaming material comprises the following steps: blending polylactic acid (PLA) and aliphatic-aromatic co-polyester toughening agent in a screw extruder; loading the extrudate into the fluid of supercritical CO2 to swell, so that the CO2 can be fully dissolved in the blended materials; then, releasing the pressure at a high rate, so as to supersaturate the CO2 in the composite material, thus achieving the thermodynamic instability; and nucleating and foaming to obtain the biodegradable PLA foaming material, wherein the toughening agent is particularly poly(butylenes adipate-co-terephthalate) (PBAT) in the invention. The method of the invention is capable of preparing the PLA foaming material with the cell diameter thereof being 0.9056mu m to 5.250mu m and the cell density thereof being 2.05*1,019 pieces / cm<3> to 2.01*1,022 pieces / cm<3>. The invention as a green and environment-friendly technique can improve the material performance and reduce the cost.

This invention relates to methods for rationally designing cell culture media for use in cell cultures, e.g., cell cultures employed in polypeptide production; cell culture media designed with the disclosed methods; methods of producing a polypeptide of interest, e.g., an antibody, using such media; polypeptides produced using the methods and media disclosed herein; and pharmaceuticals compositions containing such polypeptides. The rationally designed media contain a concentration of an amino acid that is calculated for use in cell mass, a concentration of the amino acid that is calculated for use in cell maintenance, and a concentration of the amino acid that is calculated for incorporation into the polypeptide of interest. The rationally designed media may contain a baseline-adjusted concentration, A, of at least one amino acid that is calculated according to the formula A=[(M*X)+(N*P)+(Y*M*X)]*F, wherein X is the concentration of the amino acid that is used per unit of cell mass, P is the concentration of the amino acid that is used for incorporation into the polypeptide of interest per unit of polypeptide titer, M is the multiplier for the desired peak cell density of the cell culture, N is the multiplier for the desired concentration of the polypeptide of interest, Y is the cell maintenance factor; and F is the baseline factor. This rationally designed media may also be used to produce a starting cell culture medium comprising a concentration, B, of at least one amino acid according to the formula B=[A−(Z*V)] / (1−V), wherein Z is a concentration of the amino acid in the feeding cell culture medium, and V is a volume of the feeding culture medium as a proportion of the desired cell culture medium volume.

The invention relates to a method for preparing a polymeric open cell foam material. By virtue of a supercritical fluid foaming technology, by way of mould foaming, under the premise that other performances of a polymer are not affected, nanoparticles with double effects of a nucleating agent and a cell-opening agent are added, so that the nucleation density and the aperture ratio of the polymer are remarkably improved. By adding a cell-opening auxiliary agent, on the one hand, an effect of a heterogeneous nucleating agent can be exerted to accelerate cells to be nucleated and to increase the cell density; on the other hand, the cell-opening auxiliary agent is a good cell-opening agent which promotes cell walls to break and increases the aperture ratio. The whole process provided by the invention is green and environmental-friendly and free of chemical residues; moreover, the pressure and temperature in a swelling treatment process can be adjusted to prepare the open cell foam material with different cell sizes. The polymer provided by the invention has a lot of open cells and is adjustable in cell size.

Disclosed are systems and methods for manipulating chemical, biological, and / or biochemical samples, optionally supported on substrates and / or within chambers, for example biological samples contained on chips, within biological chambers, etc. In certain embodiments, an apparatus configured to be able to position a chamber or other substrate in one or more modules surrounding the apparatus is disclosed. The apparatus may be configured to be able to move the chamber or substrate in any set of directions, such as radially, vertically, and / or rotationally, with respect to the apparatus. The apparatus may be manually operated and / or automatically controlled. Examples of modules include, but are not limited to, stacking or holding modules, barcode readers, filling modules, sampling modules, incubation modules, sensor modules (e.g., for determining cell density, cell viability, pH, oxygen concentration, nutrient concentration, fluorescence measurements, etc.), assay modules (e.g., for ELISA or other biological assays), data analysis and management modules, control modules, etc. Sensors, control systems, and the like may also be positioned to facilitate operation of the device. Certain embodiments of the invention may be used, for example, to promote or optimize chemical synthesis or cell or biological growth, for instance, for the production of compounds such as drugs or other therapeutics.

The invention belongs to the technical field of medicine, and specifically relates to a primary tumor cell culture medium, culture method and application. The primary tumor cell culture method provided by the invention comprises the steps as follows: preparing a primary cell culture medium which comprises the following components: hydrocortisone, EGF, Insulin, a ROCK inhibitor, but not contains cholera toxin, and is an improvement of the existing primary cell culture medium; culturing tumor tissue epithelial cells on laid-out trophoblast cells by using the primary cell culture medium, and enabling the tumor tissue epithelial cells to proliferate rapidly under the combined action of growth factors secreted by the trophoblast cells and nutrient factors contained in the culture medium; and digesting and sub-culturing the tumor tissue epithelial cells when the tumor tissue epithelial cells grow to a cell density of about 80% to 90%. A convenient primary cell culture method is used to obtain immortalized cells possessing the biological characteristics of a patient's own tumor, and the problem of immortalization of the primary culture of tumor cells is solved, thereby realizing personalized treatment for the patient.

A substrate with a surface-collection-layer comprising a honeycomb substrate having a porous partition wall that defines and forms cells, and a plurality of plugging portions, a surface-collection-layer being formed on a surface of the partition wall that forms the remaining cells, the thickness of the surface-collection-layer on the outlet-side open end being larger than the thickness of the surface-collection-layer in a center area of the substrate, the partition wall having predetermined thickness, porosity and pore size, the surface-collection-layer having predetermined thickness, porosity, and average pore size, and the substrate having predetermined cell density.

The high-speed centrifugation heretofore required for harvesting micro algae and cyanobacteria cultured for biofuels and other co-products is a major cost constraint. Mixing algae / cyanobacteria at high-density culture with far less alkali than previously assumed is sufficient to flocculate the cells. The amount of flocculant required is a function of the logarithm of cell density, and is not a linear function of cell density as had been thought. The least expensive alkali treatments are with slaked limestone or dolomite (calcium hydroxide and magnesium hydroxides). Further water can be removed from the floc by sedimentation, low speed centrifugation, dissolved air flotation or filtration, prior to further processing to separate oil from valuable co-products.

A load bearing structure configured to bear a load, the structure comprising a multiple cells. The load bearing structure has a length, a center, and a cell density, which varies at least along the length of the load bearing structure, which weighs at least 6 kg.

Methods for producing proteins, for example, recombinant meningococcal 2086 proteins, using fed-batch fermentation with continuous input of an inducer after achieving a threshold parameter, and optionally continuous input of a carbon source, for example, a constant rate input, to improve protein yields, as well as high density protein compositions and compositions for use in the methods of the present invention, are provided.

A single transistor vertical memory gain cell with long data retention times. The memory cell is formed from a silicon carbide substrate to take advantage of the higher band gap energy of silicon carbide as compared to silicon. The silicon carbide provides much lower thermally dependent leakage currents which enables significantly longer refresh intervals. In certain applications, the cell is effectively non-volatile provided appropriate gate bias is maintained. N-type source and drain regions are provided along with a pillar vertically extending from a substrate, which are both p-type doped. A floating body region is defined in the pillar which serves as the body of an access transistor as well as a body storage capacitor. The cell provides high volumetric efficiency with corresponding high cell density as well as relatively fast read times.

The invention relates to a discovery method of a track data hot spot based on local multilayer grids. The technical scheme includes dividing track motion space locally and in a multilayer mode according to distribution characteristics of track data and calculating cell densities; screening and expanding a density cell under a given threshold and calculating a candidate hot spot; and screening the hot spot based on the residence time of candidate hot spot track support counts and track data in the candidate hot spot. According to the method, self-adaptive effects of a divided grid coverage motion plane are good, suitable grid coverage motion can always be obtained in an iterative classification process as long as a sample point number threshold value in a cell is assigned, sample data are divided meticulously, initial classification parameters cannot affect discovery results greatly as long as a set density threshold is fixed, the method can be applied to mass data discovery, and the efficiency and the adaptability of an algorithm are guaranteed.

The invention provides a buoy for algae monitoring and early warning in a drinking water source area, which comprises a sampling tube for collecting water samples, a lifting motor for controlling the sampling tube to lift, a peristaltic pump for controlling the sampling tube to collect the water samples, an algae monitoring instrument for monitoring total algae and cell number of microcystis population in the water samples and figuring out the cell density, a data acquisition device for collecting monitored and / or calculated data, a wireless communication module for sending the data to a monitoring center, a solar panel for collecting solar energy and converting the solar energy to electric energy, a battery pack for storing the electric energy for later use, a photovoltaic controller for stabilizing a voltage and charging the battery pack, and a power panel for providing power supply for all power utilization modules. The buoy can perform monitoring by measuring the cell number of the algae, be free of interference of CDOM (colored dissolved organic matter) fluorescence, be suitable for the large concentration range of 103-1010 cells / L, be suitable for water bodies in various different algae cell concentrations and different seasons and greatly improve the accuracy and the applicability in algae monitoring in the drinking water source area.

The invention discloses a multi-task learning cell counting method based on a convolutional neural network, which is suitable for carrying out cell counting in a biological cell microscopic image withrelatively dense cells and relatively large quantity. The method comprises the following steps: preprocessing the biological cell image data set to obtain a training set and a test set; constructinga convolutional neural network model of cell counting based on multi-column feature map fusion and multi-task learning; training the convolutional neural network model, using the training set after pretreatment and the network model, and through the propagation algorithm and parameter updating, obtaining the optimized model weight parameters; testing the convolutional neural network model, using the pretreated test set and the weight parameters of the optimal network model, testing the cell picture, getting the output cell density map and the number of cell estimates, and performing evaluation. The method can improve the performance of biological cell counting and improve the accuracy rate.

Neo-cartilage constructs suitable for implantation into a joint cartilage lesion in situ and a method for repair and restoration of function of injured, traumatized, aged or diseased cartilage. The construct comprises at least chondrocytes incorporated into a support matrix processed according to the algorithm comprising variable hydrostatic or atmospheric pressure or non-pressure conditions, variable rate of perfusion, variable medium composition, variable temperature, variable cell density and variable time to which the chondrocytes are subjected.

A radial cell ceramic honeycomb structure is provided that is particularly adapted for use as a catalytic carrier or a particulate filter in an automotive or diesel exhaust system. The honeycomb structure includes a network of interconnected webs having a central axis. The network of webs includes radial webs of varying lengths, only some of which substantially extend the entire radial length of the network, and tangential webs that intersect to define rings of gas-conducting radial cells, and a rounded outer skin that surrounds the cells formed by the interconnected webs. The radial webs extending to the periphery of the network join an inner edge of the outer skin in a substantially orthogonal orientation to reduce thermally generated stresses and to increase strength of the resulting structure. The number of radial webs in the network changes along the radial length at transition zones that are defined by one of the tangential webs such that a desired cell density is achieved across the network.

A radial cell ceramic honeycomb structure is provided that is particularly adapted for use as a catalytic carrier or a particulate filter in an automotive or diesel exhaust system. The honeycomb structure includes a network of interconnected webs having a central axis. The network of webs includes radial webs of varying lengths, only some of which substantially extend the entire radial length of the network, and tangential webs that intersect to define rings of gas-conducting radial cells, and a rounded outer skin that surrounds the cells formed by the interconnected webs. The radial webs extending to the periphery of the network join an inner edge of the outer skin in a substantially orthogonal orientation to reduce thermally generated stresses and to increase strength of the resulting structure. The number of radial webs in the network changes along the radial length at transition zones that are defined by one of the tangential webs such that a desired cell density is achieved across the network.

An automated method for the production of cells and / or biomolecules such as protein or peptides includes culturing cells in at least one high cell density bioreactor, thereby fluidly connecting said bioreactor with a culture medium supply and a gas or gaseous mixture; fluidly connecting said bioreactor with a downstream unit; and growing cells to a density at least 50 million cells per ml. The total volume of the bioreactor is at least 10 liters. A system suitable for implementation of the automated method above is a small-scale and cupboard-sized system, which can be placed in a portable clean room.