94 results about "Longifolene" patented technology

The invention relates to a broad-spectrum attractant for needle leave wood pests, which is characterized by consisting of an active composition and solvent absolute ethyl alcohol. The active composition consists of alpha-pinene, beta-pinene, camphene, limonene, isolongifolenone, trans-carypohyllene, turpentine, terpinolene and beta-phellandrene. The active composition contains alpha-pinene 40%-50%, beta-pinene 20%-25%, camphene 2.5%-3.2%, limonene 11%-13%, isolongifolenone 4.5%-5.2%, trans-carypohyllene 5%-6%, turpentine 2.5%-3.5%, terpinolene 2.5%-3.5% and beta-phellandrene 0.8%-1.2%. The broad-spectrum attractant has extremely strong luring effect to monochamus alternatus adults, has high luring effect to hyposipalus gigas Linnaeus, shirahoshizo patruelis and xyleborus mutilatus blandford, has good luring effect to more than 20 varieties of pests of coleopteran, is few in using amount, remarkable in luring effect, low in cost and broad-spectrum.

The invention is suitable for the technical field of pesticide solvents, provides a plant source green and environment-friendly solvent and simultaneously provides a method for preparing the plant source green and environment-friendly solvent from destructive distillation turpentine. The plant source green and environment-friendly solvent comprises the following components in percentage by mass: 10 to 40 percent of pinene, 10 to 40 percent of D-limonene, 5 to 15 percent of longifolene, 5 to 15 percent of camphene, 3 to 15 percent of dehydroabietic acid, 1 to 10 percent of pinane and 0 to 2 percent of antioxygen. The preparation method comprises the following steps: (1) pulverizing and dehydrating the turpentine; (2) performing dry distillation and collecting lysate; (3) washing the turpentine by alkali liquor, standing the washed turpentine for fractional distillation; (4) performing dehydration; and (5) adding the antioxygen for uniform dissolution to prepare the plant source green and environment-friendly solvent. A raw material adopted by the solvent is the turpentine of pine trees, and is low in cost; the preparation method has simple process, environmental protection and lessenergy consumption; decomposition components give priority to safe and environment-friendly components, such as a terpene compound and the like and can be controlled; and the plant source green and environment-friendly solvent has low production cost, safety and environmental protection, and is beneficial for people.

The invention provides a green plant source environmental protection solvent and a preparation method thereof. The environmental protection solvent is obtained by the microwave cracking of turpentineunder the action of a certain catalyst. The solvent comprises 15wt% to 35wt% of alpha pinene, 15wt% to 40wt% of D-hesperidene, 5wt% to 15wt% of longifolene, 5wt% to 15wt% of camphene, 1wt% to 5wt% ofterpenol and 0 to 2wt% of antioxidant. Compared with the prior art, the raw material of the green plant source environmental protection solvent completely comes from the turpentine of a pine and has low price and simultaneously, a new prospect is supplied to silvichemicals. The solvent as a natural carrier of pesticide replaces an aromatic solvent and a polar solvent with poor safety environmentalprotection, reduces the release of toxic and harmful organic solvents in the environment and has mild and simple preparation condition and low energy consumption.

The invention discloses an iso-longitolanone based hexahydroquinazoline-2-amine Schiff base Zn<2+> fluorescent probe as well as a preparation method and an application thereof. The preparation method comprises steps as follows: iso-longitolanone which is a derivative of longifolene as a natural renewable resource is taken as a raw material and is subjected to condensation with benzaldehyde, and 7-benzylidene-iso-longitolanone is produced; 7-benzylidene-iso-longitolanone is subjected to condensation and cyclization with guanidine hydrochloride, and 6,6,10,10-tetramethyl-4-phenyl-5,7,8,9,10 alpha-hexahydro-6H-6 alpha,9-methano-benzo[h] quinazoline-2-amine is obtained and subjected to condensation with 2-hydroxy-1-naphthaldehyde, 1-(((6,6,10,10- tetramethyl-4-phenyl-5,7,8,9,10alpha-hexahydro-6H-6alpha,9-methano-benzo[h]-2-quinolyl) imidogen)methyl)naphthalene-2-phenol is obtained, the compound can only react with Zn<2+> to form a complex, and the complex emits green fluorescence under the irradiation of 365 nm ultraviolet light and can be taken as the fluorescent probe for Zn<2+>concentration detection.

The invention discloses an N-alkyl-1,2,3,4,5,6-hexahydro-1,1,5,5-tetramethyl-7H-2,4 alpha-methanonaphthalene-7-amine compound as well as a synthetic method and an application thereof. Longifolene serving as a heavy oil main component is relatively rich terpenoid in numerous natural extracts. The synthetic method comprises the following steps: obtaining isolongifolene through longifolene isomerization, obtaining isolongifolenone through allylic oxidation of the isolongifolene, carrying out dehydration condensation on the isolongifolenone and primary amine so as to obtain imine and obtaining the chiral N-alkyl-1,2,3,4,5,6-hexahydro-1,1,5,5-tetramethyl-7H-2,4 alpha-methanonaphthalene-7-amine compound after reduction. The compound has vey good inhibition activity on candida albicans, aspergillus niger, candida tropicalis, escherichia coli, staphylococcus aureus, pseudomonas fluorescens, bacillus subtilis and the like, a part of compound has good cancer cell proliferation inhibition activity on human breast cancer cells MCF-7, human lung adenocarcinoma cells A549, hepatoma cells HepG2 and SMMC-7721, and the N-alkyl-1,2,3,4,5,6-hexahydro-1,1,5,5-tetramethyl-7H-2,4 alpha-methanonaphthalene-7-amine compound is a potential antibacterial and bactericidal and anti-tumor compound.

The invention discloses a longifolene derivative (2, 2, 8, 8-tetramethyl-4-atrane [5. 4. 0. 11, 9] dodecane-6-alkene obtained by rearrangement and reduction reaction and by taking isolongifolene ketoxime as a raw material. Besides, bacteria such as staphylococcus aureus, Klebsiella pneumonia, Escherichia coli, bacillus proteus vulgaris and pseudomonas aeruginosa can be inhibited by the longifolene derivative, and particularly, the longifolene derivative has a superior insecticidal effect on pests such as aphids and rice planthopper.

The invention discloses a longifolene perfume material and a preparation method thereof. The longifolene perfume material comprises longifolene with content being 65-75 percent and caryophyllene with content being more than 17 percent. The preparation method of the longifolene perfume material comprises the following steps of: putting heavy turpentine in a distillation tower, conducting high-vacuum depressurized distillation, and intercepting fraction with temperature being 110-118DEG C at the top of the distillation tower at high-vacuum depressurized distillation pressure being 1Kpa. Compared with the prior art, the problem of high production cost of the existing longifolene perfume material and the preparation method thereof can be solved.

The invention provides a plant source pesticide solvent containing 15-40 percent by mass of pinene, 15-35 percent by mass of D-limonene, 2-15 percent by mass of camphene, 1-15 percent by mass of longifolene and 0-30 percent by mass of other components such as plant essential oil and / or self extract. Compared with the prior art, the plant source pesticide solvent is a vegetative solvent, and thereby the solvent is friendly to environment, can be degraded and has no pollution to the environment.

The invention discloses a hypochlorous acid fluorescent probe as well as a preparation method and an application thereof. Iso-longitolanone as a derivative of longifolene which is a natural renewableresource is taken as a raw material and subjected to condensation with 4-diethylaminobenzaldehyde, and 7-(4'-diethylaminobenzylidene) iso-longitolanone is generated; 7-(4'-diethylaminobenzylidene) iso-longitolanone and guanidine hydrochloride are subjected to condensation and cyclization, 4-(4'-diethylaminophenyl)-6,6,10,10-tetramethyl-6,7,8,9,10,10-hexahydro-5H-methenobenzo-2-quinolinamine is obtained and subjected to condensation with 2-hydroxy-1-naphthaldehyde, and a fluorescent probe compound is obtained. The compound only can be subjected to a specific reaction with hypochlorous acid, emits blue fluorescence under irradiation of 365 nm ultraviolet rays, can serve as the fluorescent probe for detecting hypochlorous acid and has good application prospect.

The invention discloses an isolongifolanone based hexahydroquinazoline-2-amine Schiff base type fluorescent material, and a synthetic method and an application thereof. The synthetic method comprises the following steps: by utilizing a natural and renewable resource, i.e., a longifolene derivative namely isolongifolanone as a raw material, carrying out condensation with 2-pyridinecarbaldehyde so as to obtain 7-(2'-pyridylmethylene)isolongifolanone; subjecting the 7-(2'-pyridylmethylene)isolongifolanone and guanidine hydrochloride to condensation and cyclization so as to obtain 6,6,10,10-tetramethyl-4-(2'-pyridyl)-5,7,8,9,10,10alpha-hexahydro-6H-6alpha,9-methanobenzo[h]quinazoline-2-amine; and carrying out condensation with 2-hydroxyl-1-naphthaldehyde so as to obtain 1-(((6,6,10,10-tetramethyl-4-(2'-pyridyl)-5,7,8,9,10,10alpha-hexahydro-6H-6alpha,9-methanobenzo[h]-2-quinolyl)imido)methyl)naphthalene-2-phenol. The compound is luminous yellow in sunlight and can emit luminous yellow green fluorescence under irradiation of ultraviolet light with a wave length of 365 nm.

The present invention relates to a catalytic process for preparation of isolongifolene using nanocrystalline solid super acid. This process is an eco-friendly, single step, solvent free catalytic process for the preparation of a tricyclic sesqui-terpene hydrocarbon, isolongifolene. More particularly, the present invention provides a process for the catalytic isomerisation of longifolene to iso-longifolene using nano-crystalline sulfated zirconia as a solid super acid catalyst.

The invention discloses a purification method for longifolene, belonging to the technical field of deep processing of turpentine. The purification method comprises the following steps of placing longifolene to be treated, water, acetic acid, p-dihydroxybenzene and p-toluenesulfonic acid into a reaction kettle under a stirring condition, heating to 40-60 DEG C, continuing to stir, stopping stirring after 6-10 hours, standing for separating a material layer, and then, distilling after washing the obtained material. The purification method has the advantages of mild reaction conditions, simplicity and convenience in process and flow, few utilization equipment and low cost.

The invention relates to a dendroctonus armandi dendroctonus attractant. The active ingredients of the dendroctonus armandi dendroctonus attractant are aggregation pheromone and host kairomone. The dendroctonus armandi dendroctonus attractant comprises the following components with the following releasing rates: trans-verbenol (tV) being 0-10mg / d, 3-methyl-2-cyclohexene-1-alcohol (SD) being 0-3mg / d, 7-ethyl-5-methyl-6, 8-bioxygen bicyclo-[3,2,1] octane (EBV) being 0-3mg / d and 1-methyl-2-cyclohexene-1-alcohol (MCOL) being 0-3mg / d, alpha-pinene being 0-500mg / d, delta3-carene being 0-100mg / d, beta-pinene being 0-100mg / d, limonene being 0-100mg / d and beta-myrcene and (+)-longifolene being 0-100mg / d. The dendroctonus armandi dendroctonus attractant can effectively attract dendroctonus armandi dendroctonus and has extremely high in attraction to bisexual adult insects; the preparation technology is simple and the dendroctonus armandi dendroctonus attractant is convenient to use.

The invention discloses a method for continuously separating high-purity longifolene from heavy turpentine. The steps of activated clay decoloration and thermal pretreatment are performed on raw materials, and discharging is performed through the pressure different between a tower kettle and a residual liquid tank under the condition of not cooling a rectifying tower or breaking the vacuum, so that energy is saved, and continuous operation of intermittent distillation is realized. The longifolene content of a longifolene product obtained by using the method is greater than 75 percent, and can be up to 95 percent in maximum.

A lubricating oil composition of the invention contains longifolene represented by the following formula (1), that is, (1S,3aR,4S,8aS)-4,8,8-trimethyl-9-methylene-decahydro-1,4-methanoazulene.

The invention discloses a method and a device for separating high-purity longifolene. Thermal pretreatment is carried out on heavy turpentine, continues operation of reduced pressure distillation of a rectifying tower is realized through discharging of pressure difference between a tower kettle and a residual liquid tank, the rectifying tower does not need to be cooled and elimination of vacuum discharging is not needed after the fractionation of once feeding is completed, the vacuum of the residual liquid tank is reduced to be lower than or equal to the vacuum of the tower kettle through a vacuum pump, residual liquid in the tower kettle is discharged to the residual liquid tank through the pressure difference between the tower kettle and the residual liquid tank, and then raw materials are absorbed in the tower kettle by utilizing negative pressure between the tower kettle and a raw material storage tank, so that seamless cyclic operation of the working procedures such as feeding, fractionation and discharging is realized, and the energy sources are saved. The content of longifolene in longifolene products obtained by the method is greater than 98%.

The present invention relates to a catalytic process for preparation of isolongifolene using nanocrystalline solid super acid. This process is an eco-friendly, single step, solvent free catalytic process for the preparation of a tricyclic sesqui-terpene hydrocarbon, isolongifolene. More particularly, the present invention provides a process for the catalytic isomerisation of longifolene to iso-longifolene using nano-crystalline sulfated zirconia as a solid super acid catalyst.

The invention belongs to the field of synthetic biology, and relates to genetic engineering bacteria producing longifolene. The genetic engineering bacteria is a recombinant Escherichia coli containing related longifolene anabolic pathway genes (including farnesyl pyrophosphate synthetase gene ispA and longifolene synthase gene lgfS); and the recombinant Escherichia coli is subjected to base platemodification of optimized endogenous MEP synthesis pathway and the optimization of the metabolic pathway by introducing key enzyme genes in the heterologous MVA pathway, so that the obtained geneticengineering bacteria producing longifolene has a higher yield of longifolene. The genetic engineering bacteria is connected to a fermentation medium, an inducer IPTG is added, long-chain n-alkanes areadded during the fermentation and culture process for two-phase extraction fermentation, the yield of a longifolene shake flask reaches 1.77mg / L, and the industrial application prospects are good.

The invention discloses a method for preparing epoxy caryophyllene from heavy turpentine oil and separating longifolene. The method comprises the following steps: putting heavy turpentine oil into a reaction container, adding organic solvent ethyl acetate, a catalyst and an oxidant; under normal pressure and at the normal temperature, performing epoxidation reaction on caryophyllene in heavy turpentine oil, wherein the caryophyllene is almost not oxidized; then, cooling a reaction mixture, separating out the catalyst, filtering and recycling, and removing a water phase of filtrate through phase separation, distilling an organic phase under reduced pressure to recycle the organic solvent, and performing fractional distillation and separation under reduced pressure to obtain oily liquid products, namely longifolene and epoxy caryophyllene, further adding a few crystal seeds into the epoxy caryophyllene oily liquid to crystallize and separate out the crystal seeds, filtering and collecting crystals under reduced pressure, and drying in vacuum to obtain an epoxy caryophyllene crystal product. According to the method disclosed by the invention, selective catalytic oxidizing reaction process is adopted, the problem that the existing high-purity longifolene is difficult to separate is solved, the epoxy caryophyllene is prepared, and therefore, the method has the advantages of simple operation process, gentle reaction condition, environment-friendly reaction process and the like, and is suitable for industrial production.

The invention discloses a preparation method of high-purity longifolene. The preparation method comprises the following steps: placing mixture of 65-70 percent by weight of longifolene and 10-30 percent by weight of caryophyllene into a reaction container; adding a catalyst to perform caryophyllene polymerization reaction, wherein the reaction heating temperature is 80-110 DEG C and the reaction time is 3-6 hours; filtering; recovering the catalyst; performing reduced pressure distillation; rectifying to obtain the longifolene finished product with content of 85-95 percent, wherein the catalyst is macroporous weakly acidic acrylic acid cation exchange resin D113; the weight ratio of the mixture to the catalyst is 1:0.05-0.1. Compared with the prior art, the preparation method has the advantage that the problems of difficulty in separation of the high-purity longifolene and high production cost existing in the prior art can be solved.

The invention discloses an ester-producing Wickerhamomyces anomalus strain and application thereof in pickles, and belongs to the technical field of microorganisms. The strain is Wickerhamomyces anomalus YYB24, and is preserved in the China General Microbiological Culture Collection Center on August 28, 2020, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.20577. The strain has relatively strong ester production capacity and excellent fermentation performance. When the Wickerhamomyces anomalus YYB24 is inoculated for fermentation, the sensory score of pickles is obviously increased, the variety of ester substances is increased by 10, the relative content is increased by 146.45%, the relative contents of important flavor substances such as phenethyl alcohol and longifolene for pickles are also obviously increased, and the result shows that the Wickerhamomyces anomalus YYB24 has a huge potential when being applied to pickle production.

The invention discloses a method for synthesizing isolongifolene by catalytically isomerizing longifolene with solid acid. The method comprises the following steps: mixing longifolene and glacial acetic acid; putting the mixture into a reaction container; adding a catalyst to perform an isomerization reaction; filtering after the reaction is finished; recycling the catalyst; distilling to recycle a solvent; rectifying to obtain an isolongifolene finished product. The method is characterized in that the catalyst is solid acid D72 macroporous strong acid ion exchange resin; the weight ratio of the longifolene to the glacial acetic acid to the catalyst is 1:(0.4-1.2):(0.1-0.3); the temperature in the reaction container is 60-110 DEG C, and the reaction time is 4-6 hours. The method can solve the existing problems of high cost and severe pollution for producing isolongifolene or isolongifolenone.

The invention discloses thermal insulation coating and belongs to the technical field of coating. The coating is prepared from the raw materials of, by weight, 80-100 parts of epoxy resin, 15-20 parts of pinonic acid, 10-15 parts of longifolene, 30-45 parts of pore-closed perlite, 5-8 parts of nanometer silicon carbide, 4-6 parts of cowhide cream, 8-15 parts of nanometer bamboo carbon fiber, 5-40 parts of pigment, 40-45 parts of ethylene glycol, 1-5 parts of silicone oil and 60-75 parts of water. The thermal insulation coating has a better thermal insulation effect.

A lubricating oil composition of the invention contains longifolene represented by the following formula (1), that is, (1S,3aR,4S,8aS)-4,8,8-trimethyl-9-methylene-decahydro-1,4-methanoazulene.

The invention relates to a pesticide solvent. The pesticide solvent is prepared from the following raw materials in parts by weight: 2-30 parts of jatropha curcas oil, 1-30 parts of jatropha curcas oil methyl ester, 5-20 parts of isopropanol, 5-10 parts of glycol, 3-13 parts of azone, 1-23 parts of camphene, 5-20 parts of longifolene, 2-27 parts of castor oil, 4-10 parts of palm oil and 0.4-2 parts of tert-butylhydroquinone. The solvent is environment-friendly and degradable, free from pollution on the environment and low in cost, and can be widely applied to various pesticide preparations or prepared into a pesticide mixing auxiliary.

This invention relates to one chemical dyeing method to identify the diffusion of breeding pine wire worm, which comprises the following steps: a, solving the stimulant into dimethyl sulfoxide liquid with two weight proportion as 2-50:100 and element A, B and C weight proportion as 54-90:9-43:1-5 with A for alpha- firpene, B for beta- firpene and C for longifolene; b, dripping the stimulant into the water liquid with the worm and adding 10 mu L coomassie brilliant blue with intensity of 0.2% and putting in room temperature for 24 hours and observing under microscopes for 24 hours.

The invention discloses a novel method of preparing isolongifolenone. The method comprises the following steps: by taking isolongifolene (an isomerized product of longifolene) as a raw material, copper power as a catalyst, tertiary butanol as a solvent and tert-butyl hydroperoxide as an oxidizing agent, oxidizing the longifolene in one step to obtain isolongifolenone; at the end of reaction, filtering out the copper powder; evaporating out tertiary butanol at normal pressure; and then carrying out reduced pressure distillation and collecting an isolongifolenone fraction. The novel method disclosed by the invention is simple in operating process, strong in oxidizing selectivity and good in catalytic effect. The catalyst and the solvent can be repeatedly used, and the catalyst is low in price and abundant. The novel method overcomes the deficiencies of existing preparation methods,such as expensive catalysts, complex process and poor reaction selectivity.

The present invention relates to a catalytic process for preparation of isolongifolene using nanocrystalline solid super acid. This process is an eco-friendly, single step, solvent free catalytic process for the preparation of a tricyclic sesqui-terpene hydrocarbon, isolongifolene. More particularly, the present invention provides a process for the catalytic isomerisation of longifolene to iso-longifolene using nano-crystalline sulfated zirconia as a solid super acid catalyst.

The invention discloses a method for producing terpene resin by using longifolene as a raw material, and belongs to the technical field of rosin deep processing. The present invention adopts the following raw materials in parts by weight: 50 to 55 parts of longifolene, 1 to 5 parts of oligomeric diol, 5 to 8 parts of diisocyanate, 0.5 to 1 part of organosilicon-modified polysiloxane and 0.05 part to 0.1 part of anhydrous aluminum trichloride; including putting the above-mentioned raw materials longifolin, oligomeric diol, diisocyanate, organosilicon-modified polysiloxane and anhydrous aluminum trichloride into the reaction kettle and mixing uniformly, And heat-preserve and react at 80° C. to 95° C. for 10 minutes to 20 minutes, and then carry out distillation. The present invention adds a certain proportion of oligomeric diol, diisocyanate, organosilicon-modified polysiloxane and anhydrous aluminum trichloride in the preparation process, thereby effectively shortening the production cycle of the product terpene resin and reducing its cost .

The present invention discloses a longifolene purification method, and belongs to the technical field of turpentine deep processing. The longifolene purification method comprises: stirring longifolene to be treated, an acetic acid solution, hydroquinone and p-toluenesulfonic acid, carrying out standing separation to obtain a material layer after stopping the stirring, sequentially carrying out water washing and rectification on the obtained material, transferring into another reactor, carrying out a reaction of the material and a solid phosphoric acid catalyst, and carrying out pressure reducing distillation on the substance obtained from the reaction. According to the present invention, the longifolene purification method has advantages of mild reaction condition, simple process and simple procedure, less equipment, and low cost; and with the longifolene purification method, the longifolene product purity can achieve more than or equal to 90%.