230 results about "Order Coleoptera" patented technology

Disclosed are Coleopteran-toxic B. thuringiensis delta -endotoxins, nucleic acid sequences, and transgenic plants expressing these genes. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle (Lymantria dispar) and Southern Corn Rootworm (Diabrotica undecempunctata). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby.

The invention relates to a highly effective bacillus thuringiensis cry8G gene and albumen for killing coleoptera pests and application thereof, pertaining to biological control technical field. The invention provides nucleotide sequences of the bacillus thuringiensis cry8G gene which is high poisonous to the coleoptera pests and amino acid sequences of the codes of the protein, and provides nucleotide sequences of the bacillus thuringiensis cry8G gene which is designed artificially and used for transgenic plants and amino acid sequences of the codes thereof. Therefore, toxicity to related pests is presented by the sequences through the genes or the artificial designed sequences of transgenic microbes and plants so as to overcome or delay the generation of drug-resistance of the pests for engineering bacteria and the transgenic plants.

The present invention provides isolated polynucleotide sequences encoding ET37, TIC810 and TIC812 proteins from Bacillus thuringiensis, and nucleotide sequences for use in expressing TIC809, ET37, TIC810 and TIC812, and fusions of various insecticidally effective combinations of these proteins such as TIC 127, in plants. Methods of making and using the polynucleotide sequences and the proteins in the development of transgenic plant cells and transgenic plants exhibiting improved insect resistance against (1) Coleopteran insects including Western Corn Rootworm (Dibrotica virgifera), Southern Corn Rootworm (Dibrotica undecempunctata), Northern Corn Rootworm (Diabrotica barbed), Mexican Corn Rootworm (Diabrotica virgifera zeae), Brazilian Corn Rootworm (Diabrotica balteata) and Brazilian Corn Rootworm complex (Diabrotica viridula and Diabrotica speciosa), and against Hemipteran insects such as Lygus bugs, are disclosed.

The invention relates to a broad-spectrum attractant for needle leave wood pests, which is characterized by consisting of an active composition and solvent absolute ethyl alcohol. The active composition consists of alpha-pinene, beta-pinene, camphene, limonene, isolongifolenone, trans-carypohyllene, turpentine, terpinolene and beta-phellandrene. The active composition contains alpha-pinene 40%-50%, beta-pinene 20%-25%, camphene 2.5%-3.2%, limonene 11%-13%, isolongifolenone 4.5%-5.2%, trans-carypohyllene 5%-6%, turpentine 2.5%-3.5%, terpinolene 2.5%-3.5% and beta-phellandrene 0.8%-1.2%. The broad-spectrum attractant has extremely strong luring effect to monochamus alternatus adults, has high luring effect to hyposipalus gigas Linnaeus, shirahoshizo patruelis and xyleborus mutilatus blandford, has good luring effect to more than 20 varieties of pests of coleopteran, is few in using amount, remarkable in luring effect, low in cost and broad-spectrum.

The invention discloses an attractant as well as an application, a preparation method and an application method of the attractant, relates to the technical field of agriculture and aims at solving the problems of an existing method for preventing and treating insect pests such as potosia brevitarsis by use of pesticides, for example, pollution on the human environment and harm to the human health. The attractant is prepared from the following raw materials in parts by weight: 1-4 parts of sugar, 1.5-4.5 parts of vinegar, 0.5-2.5 parts of wine, 0.1-0.3 parts of dipterex and 7-12 parts of water. The attractant is mainly used for attracting and killing coleopteran pests such as potosia brevitarsis, and is good in attracting and killing effect without causing harm to the human environment and the human health.

The present invention discloses a synergistic insect disinfestation composition containing clothianidin and chlorantraniliprole, and an application thereof. The synergistic insect disinfestation composition adopts clothianidin and chlorantraniliprole as main effective components, wherein a weight ratio of the clothianidin to the chlorantraniliprole is 1-100:0.5-80. The synergistic insect disinfestation composition has effects of prevention and control spectrum expanding, synergy and medication cost reduction, can be prepared into an emulsifiable concentrate, wettable powder, a microemulsion, an aqueous emulsion, a suspending agent and water dispersible granules, and is provided for preventing and controlling Lepidoptera, Hemiptera, Hymenoptera, Coleoptera and a plurality of pests.

The invention provides a biological insecticide containing methoprene as well as preparation and application methods thereof. The biological insecticide is prepared from the following components: 0.01-10% of methoprene, 0.01-5% of surfactant, 0.01-10% of light stabilizer and 75-99% of solvent. The biological insecticide has the advantages that: by adopting the mixed solvent and the surfactant at the same time, the effective ingredients can be uniformly dispersed in the solution, and the uniformity of the effective ingredients in the using process can be ensured; the light stabilizer is added, thus the attenuation rate of methoprene is obviously reduced, the storage life of the insecticide is prolonged, and the using effect of the insecticide is enhanced; and the biological insecticide has very obvious killing effects on mosquitoes and flies in water as well as beetles, flour moths and other lepidoptera, diptera, coleoptera, homoptera and other insects in grain and tobacco, and can be applied to prevention and control of mosquitoes and flies in tap water, storage of grain and tobacco as well as disinfection and insect disinfection of carpets and the like.

The invention relates to a pesticide insecticidal composition, the effective components of which contain (A) spirotetramat and (B), i.e. one of amide insecticides. Specifically, A and B are in a mass percentage of 1-80:80-1, and the content of A and B in the compound preparation is 1-80%. The compound pesticide composition prepared by the invention can be prepared into missible oil, suspending agents, wettable powder, water dispersible granules, emulsions in water and other dosage forms by methods well known to technicians in the field. The insecticidal composition provided by the invention is suitable for controlling piercing-sucking mouthpart pests in vegetables and fruits, and is especially suitable for controlling aphids, whiteflies, mealybugs, scale insects, plant hoppers, thrips, Empoasca pirisuga Matumura, as well as lepidoptera, coleoptera pests like diamondback moths, cotton bollworms and the like.

The subject application concerns novel materials and methods for controlling non-mammalian pests. In a certain embodiments, the subject materials and methods for the control of coleopteran or lepidopteran pests are provided. The subject application also provides pesticidal proteins and compositions comprising pesticidal proteins that are derived from Beauvaria bassiana. These proteins have molecular weights that are greater than about (or greater than) 5000 daltons. In a preferred embodiment, the subject invention concerns plants cells, plant parts or plants to which the pesticidal proteins, or compositions thereof, have been applied.

The invention relates to the Cry8Na1 gene of Bacillus thuringiensis (Bt), expression protein and application thereof, which belongs to the field of biological control. The preservation number of Bt strains BTQ52-7 is CGMCC No.4188. The amino acid sequence of the insecticidal protein is shown in SEQ ID No.2. Preferred nucleotide sequences of genes encoding the above insecticidal protein is shown in SEQ ID No.1. The above genes with high virulence to coleoptera insects are applicable for transforming micro-organisms and plants. Thus the transformed micro-organisms and plants are resistant to relevant insects. The development of drug resistance of insects to engineering bacteria and transgenic plants can also be overcome or delayed.

The invention discloses organic biological pesticide fertilizer. The organic biological pesticide fertilizer is characterized by comprising biotic components and plant components, is not harmful to human and environment, has double effects of fertilizer and insect pest killing, promotes growth of plant, protect agroecological environment, and can replace harmful chemical pesticide and chemical fertilizer. The pesticide fertilizer has a special function of killing insect pests and preventing diseases, and the comprehensive functions of promoting growth of plant, increasing the set of fruit, and improving yield and quality, and is suitable for rice, wheat, cotton, rape, soybean, peanut, corn, fiber crops, watermelon, fruit trees, vegetables, tea, medicinal materials, pasture, nursery stock and the like and has the effect of increasing the yield of walnut and extremely high sensitivity to lepidoptera, orthoptera, diptera, coleopteran, thysanoptera, hymenoptera and acarina insect pests, such as prodenia litura, asparagus caterpillar, cabbage caterpillar, aphid, walnut contarinia citri barnes and locust.

The invention relates to an insecticidal compound containing clothianidin and bifenthrin as effective components and an application thereof. The insecticidal compound provided by the invention is mainly used in the research and application of controlling whitefly and aphid. The insecticidal compound provided by the invention is characterized by containing the active components of clothianidin and bifenthrin. The compound contains 0.1-50 wt% of clothianidin, 50-0.1 wt% of bifenthrin and the balance being auxiliary components which are allowed and can be accepted in pesticides. The insecticidal compound provided by the invention can be applied to control a plurality of Hemiptera, coleopteran and Lepidoptera insects, such as whitefly, plant hopper, aphid, leafhopper, thrip, Psylla chinensis Yang et Li, Myzus persicae, yellow aphids, Myzus persicae, pod borer and the like, on paddy rice, vegetables, fruit trees and other crops. The insecticidal compound provided by the invention has an outstanding effect of controlling lepidoptera insects and piercing-sucking insects on crops.

The invention relates to a clothianidin synergia solubility granula and a preparation method thereof. The granula is prepared from the following components by weight percent: 1-20 percent of clothianidin, 1-10 percent of a dispersing agent, 1-5 percent of a wetting agent, 2-4 percent of a binder, 1-3 percent of a stabilizer, 1-3 percent of a synergist and the balance of fillings. The preparation method comprises the following steps of: carrying out coarse grinding on the clothianidin, dispersing agent, wetting agent, stabilizer and fillings, stirring and mixing, then carrying out ultrafine grinding to obtain ultrafine powder with the grain size less than 15 micrometers; and adding a binder for pelleting, screening by using a vibrating screen, and drying to obtain a product. The clothianidin synergia solubility granula has the advantages of the solid preparation and liquid pharmaceutical preparations; at the same time, due to the addition of synergist, the clothianidin synergia solubility granula has obviously control efficiency on the common crop injurious insects such as Hemiptera, coleopteran, diptera and certain lepidoptera pests, has the insecticidal efficacy improved by 1.4-1.5 times compared with that of the 50% clothianidin aqueous dispersion granula, and improves the utilization rate of the active constituent clothianidin.

A novel gene encoding a Coleopteran inhibitory Bacillus thuringiensis insecticidal crystal protein is disclosed. The protein, tIC851, is insecticidally active and provides plant protection from at least cotton boll weevil, Anthomomus grandis, when applied to plants in an insecticidally effective composition.

The invention discloses a liquid formulation for trunk injection contained with extract of willow root for landscape plant insect attack and preparation and application thereof. The liquid formulation for trunk injection for landscape plant insect attack comprises the extract of the willow root, emamectin benzoate, surface active agent, anti-decomposing agent and cosolvent. The mass percents of the extract of the willow root, the emamectin benzoate, the surface active agent, the anti-decomposing agent and the cosolvent are 4 to 20%, 0.75 to 1.5%, 1 to 10%, 0.35 to 0.5% and 10 to 20% in sequence, and the margin is water. The liquid formulation for trunk injection for landscape plant insect attack is contained with the extract of the willow root, the insecticidal spectrum is wide, and the functional mechanism is unique, so that the drug resistance of the injurious insects can be solved effectively, features of contacting toxicity, stomach toxicity, systematic action and the like are possessed, drug application is conducted in an anhydrous and non-contact mode, the liquid formulation is safe to human and environment, and purposeful prevention and cure on the injurious insects like hemiptera, coleoptera, homoptera, diptera and some of lepidoptera at the places of forests, urban gardening, gardening trees in a scenic spot, border trees and protection forest are achieved.

The invention discloses a new beauveria bassiana strain XWY-1 which has high poisoning capability on forestry important insects and especially on coleoptera chrysomelidae insect pyrrhalta aenescens. Toxicity tests show that XWY-1 strain has extremely toxicity on coleoptera chrysomelidae insect pyrrhalta aenescens, and therefore beauveria bassiana XWY-1 can be prepared into pesticides for controlling forestry insects, and thus the use amount of pesticides is reduced, the environmental pollution is reduced, natural enemies are protected, and beauveria bassiana XWY-1 has important economical value and application prospect.