A soil respiration measurement cultivation and gas collection device and its measurement method, relating to a soil measurement device and its measurement method, the device comprises a culture bottle, a rubber cap, a narrow neck of a culture bottle, a connecting tube, a culture bottle mouth, and a rubber stopper , two-way valve, gas sampler; the rubber stopper is connected to the top opening of the culture bottle, one end of the communication tube passes through the rubber stopper to connect with the inside of the culture bottle, the other end is connected to the two-way valve, and the rubber cap is connected to the neck of the side wall of the culture bottle , the gas sampler is connected with the two-way valve; the method includes weighing the soil sample, connecting the culture device, covering the rubber stopper, installing the connecting pipe, carrying out airtight culture, gas collection, and measuring CO in the gas. 2 Content; re-cultivation, soil respiration intensity measurement. The design of the whole device of the present invention is simple; the loss caused by steps such as pipetting and titration in the traditional method and the test operation error are avoided in the process of measuring the respiratory intensity of the method, and the measured data is reliable.