The invention relates to a method for measuring forest soil NO2 emission. The method comprises the five following steps: firstly, selecting a sampling point; secondly, collecting a soil sample; thirdly, measuring the content of soil dissolved organic nitrogen (DON); fourthly, establishing a linear relation, wherein a linear relation function of forest soil NO2 emission amount and DON is: Y=0.0435X-0.1361; and fifthly, actually measuring the forest soil NO2 emission amount, wherein the content (mg.kg<-1>) of DON in the forest soil to be measured which is used as X is substituted to the function relation formula to solve for Y, i.e. the forest soil NO2 emission amount with the unit of mg.m<-2>.h<-1>. Compared with a static chamber method, the method is used for calculating the forest soil NO2 emission amount through measuring the DON, the field work load is reduced, the measuring cost is lowered, the batch quantity analysis can be carried out, the measuring work efficiency is improved, the obtained numerical error range is small, and the reliability is better.