The invention provides a polyurethane chemical grouting material and a preparation method thereof. At first a hydroxyl-terminated polyurethane prepolymer is prepared: hydrophilic polyol or small molecular polyether and isocyanate are mixed and stirred at normal temperature, and heating and heat preservation processes, etc. are carried, and when -NCO percent is detected to be near zero by central control, the reaction is stopped, and the hydroxyl-terminated polyurethane prepolymer is prepared. Secondly, the hydrophilic polyurethane prepolymer is prepared: the macromolecular polyether and the hydroxyl-terminated polyurethane prepolymer prepared through the reaction are stirred and mixed evenly in a stirring reaction kettle which is provided with a condenser. At normal temperature, isocyanate is added into the kettle for stirring and heating and heat preservation processes, etc. are carried out; -NCO percent is detected, and the reaction is stopped when the -NCO percent is between 5.6 percent and 6.0 percent. Lastly the polyurethane grouting material is prepared: a diluting agent is added into the prepared polyurethane prepolymer, and the stirring process is started; foam stabilizer, polyurethane special catalyst and plasticizer are added into the prepolymer at the same time. The mono liquid grouting of the polyurethane chemical grouting material of the invention has simple construction, easy operation, and gel is nonpoisonous, and can be used for water shutoff of water supply lines, etc.