The invention discloses a test strip for fluorescence immunochromatography of myeloperoxidase. The test strip comprises a base plate, a sample pad, a binding pad, a nitrocellulose membrane and an absorbent pad, and the sample pad, the binding pad, the nitrocellulose membrane and the absorbent pad are assembled on the base plate in a sequential overlapping manner, wherein the absorbent pad and thebinding pad are respectively pressed on two ends of the nitrocellulose membrane in an overlapping manner, and a detection area is formed on the surface of the nitrocellulose membrane; the sample pad is pressed on the binding pad in an overlapping manner, and a myeloperoxidase antibody-fluorescent microsphere compound is immobilized on the binding pad; and the nitrocellulose membrane in the detection area is coated with a detection line formed by a monoclonal antibody for recognizing another epitope of myeloperoxidase and a control line formed by a goat anti-mouse IgG polyclonal antibody. The test strip has the advantages of high sensitivity, high stability, realization of the detection linearity being 3.125-600 ng/ml, no non-specificity, short detection time of 5 min, realization of bedside quick test, and great improvement of the clinical diagnosis efficiency.