The invention discloses a kit for HPV (
human papillomavirus)
nucleic acid detection based on a universal fluorescent
nanoparticle counting method in combination with
CRISPR / Cas12a recognition, and belongs to the field of
nucleic acid detection.The kit comprises crRNA, Cas12a, a
buffer solution 3.1, an
RNA enzyme inhibitor, Target, HPV-MB and HPV-FNP. According to the kit, the Cas12a
nucleic acid molecule recognition characteristic, the fluorescent
nanoparticle characteristic and the base complementary
pairing hybridization and
magnetic separation principle are utilized, so that the
nucleic acid molecule recognition characteristic of the crRNA, the fluorescent
nanoparticle characteristic and the base complementary
pairing hybridization and
magnetic separation principle are utilized; fluorescent nanoparticle counting and
CRISPR detection are combined for the first time, universal
virus nucleic acid detection is achieved, operation is easy and convenient, observation is easy, and a
CRISPR / Cas probe design method is widened. By taking HPV16 and 18
virus nucleic acids as examples,
nucleic acid detection schematic results show that 1 pM can be detected at least. And the HPV16
virus and the HPV18 pseudovirus are selected as examples to carry out actual sample detection and
verification.