The invention discloses a multi-line laser radar and multi-path camera mixed calibration method. The method comprises the following steps of S1, collecting the original image data of a multi-path camera, the point cloud data of a multi-line laser radar and the point cloud data of a static laser radar; S2, solving an internal reference model of each camera; S3, subjecting images acquired by each camera to de-distortion treatment and obtaining corrected images; S4, registering the point cloud data of the static laser radar into the point cloud coordinate system of the multi-line laser radar; S5, acquiring the position (Xs,Ys,Zs) of each camera in the point cloud coordinate system of the multi-line laser radar in the point cloud data which are well registered in the step S4; S6, selecting the pixel coordinates (u,v) of at least four target objects in the corrected images of each camera and the corresponding three-dimensional coordinates (Xp,Yp,Zp) of the target objects in the point cloud with the multi-line laser radar as an coordinate origin; S7, according to the internal reference model of each camera, the position (Xs,Ys,Zs) of each camera, the pixel coordinates (u,v) of target objects corresponding to the camera, and the corresponding three-dimensional coordinates (Xp,Yp,Zp), establishing a collinear equation. In this way, the attitude angle elements of each camera and the cosines of the camera in nine directions can be figured out. Therefore, the calibration is completed.