The invention relates to a three-dimensional printing method and device for tissue/organ chip integrated manufacturing. The three-dimensional printing method comprises the following steps: (1), designing the three-dimensional structural drawing of a three-dimensional chip, and converting the three-dimensional structural drawing into a slice layer graphic file format; (2), starting a three-dimensional printing device, and absorbing printing ink into all spraying heads; leading in slice layer graphic files; (3),respectively printing a main body material printing ink, a sacrificial material printing ink and different cell printing ink into pre-designed positions of a bottom plate system through the three-dimensional printing device; (4), repeating the step (3) and accomplish printing of a three-dimensional chip structure layer by layer and in an accumulating manner until the printing to a slice layer graphic file is finished; (5), heating or refrigerating the three-dimensional chip integrally printed, so as to enable a channel sacrificial material to be changed into a sol state; (6), using a pipette to absorb the channel sacrificial material in the sol state, removing the channel sacrificial material, and forming an integrated three-dimensional chip structure; (7), carrying out crosslinking on the cell printing ink material not molten, and carrying out culture medium perfusion.