277results about How to "Improve assay efficiency" patented technology

The invention provides a method for measuring a 2D angle of arrival (AOA) of coherently distributed signals. The method belongs to the technical field of wireless mobile communication technology. The method includes the steps of setting a stereoscopic antenna array composed of three parallel uniform linear arrays; establishing a data vector matrix and a propagator matrix of all the coherently distributed signals; eliminating aperture loss; establishing rotation matrixes and making pairing and combination of characteristic values of the rotation matrixes; and determining a central azimuth angle and a central pitch angle of the distributed signals. In the invention, the propagator is determined with received data firstly, then the three rotation matrixes are determined with the propagator, and finally the central azimuth angle and the central pitch angle of the distributed signals are comprehensively determined through automatically pairing the characteristic values of the three rotation matrixes by using the method in the invention, thereby improving the measuring precision to about 10 times higher than the precision of the prior art. Therefore, the method in the invention has characteristics of simplicity, high reliability, high performance in lowering the calculation processing capacity and complexity degree substantially, high measuring efficiency, high measuring precision degree and so on.

The invention discloses a method for determining four hormones in a rubber tree through high-efficiency liquid chromatography. The method provided by the invention comprises the following steps of: (1) sample pretreatment: to be specific, grinding rubber tree tissues with liquid nitrogen, then placing in leaching liquor for extraction, centrifuging, separating a centrifuged liquid supernatant to obtain an aqueous phase, enabling the aqueous phase to pass through a chromatographic column, and eluting with methyl alcohol to obtain a sample to be tested; and (2) high-efficiency liquid chromatography detection: adopting an inverse-phase C18 chromatographic column, taking a mixed liquid of the methyl alcohol, acetonitrile and phosphate buffer as a mobile phase and 254nm as testing wavelength, and detecting auxin, zeatin, activol and abscisic acid in the sample to be tested obtained in the step (1). The method provided by the invention has the advantages that the four hormones can be detected, the extraction flow time is short, the determination accuracy rate is high, and the determination and analysis efficiency is improved. The method is a good method for researching the high-yield fast-growing mechanism of the Brazil rubber tree and the plant hormones in the cambium differentiation process.

The invention belongs to a measuring method of synthetic aperture radar (SAR) non-fuzzy Doppler center frequency, comprising: compressing range direction pulse; obtaining initial slope; converting a frequency domain and a time domain; obtaining the waveform entropy of a sequence; determining the minimum waveform entropy; and determining the non-fuzzy Doppler center frequency. The invention utilizes the geometrical characteristics of the synthetic aperture radar echo on range time and azimuth time domains and the self information of a mobile platform to repeatedly correct range ambulating trajectory so as to determine the minimum entropy, thus measuring whether the trajectory is well corrected or not; the slop ratio of the corresponding trajectory to the wavelength of an emitted signal is used for obtaining the non-fuzzy Doppler center frequency; and the measured frequency is improved by more than 5 times compared with Laden conversion. Thus, on the aspect of bi-static forward-looking and squinting synthetic aperture radar (SAR) Doppler center frequency, the invention has the characteristics of simple and accurate measurement, short processing time, high efficiency, strong instantaneity and capability of providing accurate and reliable data for subsequent high-precision imaging and motion compensation.

The invention discloses a representation method of oil-gas exploration and oil-gas reservoir by taking vital bacterium abnormality and dead bacterium abnormality of methane-oxidizing bacteria as indicators, comprising the following steps: collecting a sample above an oil-gas exploration and oil-gas reservoir representation area according to the specific grid degree, the sampling depth and the sampling amount, packaging and saving the sample according to the specific condition; adopting a conventional microorganism counting method to obtain the vital bacterium quantity and the dead bacterium quantity of the methane-oxidizing bacteria in the sample; drawing an equal bacterium line of the vital bacteria and an equal bacterium line of the dead bacteria on a map, a topographic map, a geologic map or a topography-geologic map to obtain the vital bacterium abnormality and the dead bacterium abnormality; comprehensively comparing the vital bacterium abnormality and the dead bacterium abnormality; and estimating and predicting the underground oil-gas resource condition of the oil-gas exploration and oil-gas reservoir representation area, or extracting an exploitation scheme of the oil-gas reservoir according to the characteristics of the vital bacterium abnormality and the dead bacterium abnormality and the differences of the vital bacterium abnormality and the dead bacterium abnormality, and combining the results of geological exploration, geochemical exploration and geophysical exploration.

The invention discloses a measuring method of carbon, hydrogen and nitrogen contents in biomass fuel. The measuring method comprises the following steps of burning a sample to be measured, collecting mixed gas generated by burning, measuring and calculating full carbon, full hydrogen and full nitrogen percentage contents of the sample to be measured, and calculating the carbon, hydrogen and nitrogen contents in the biomass fuel to be measured. The method has the advantages that the measuring accuracy of the method is higher than that of an infrared-thermal conductance method of a coal sample, the measuring efficiency of the method is higher than the combined measuring efficiency of standard measuring methods, namely a semimicro Kelvin method and a three-section furnace method, and the method is applicable to quickly and efficiently measuring the carbon, hydrogen and nitrogen contents in the biomass fuel, can achieve high accuracy of measuring results and is low in implementation cost.

The invention discloses an automatic measurement device and measurement method of underground coal mine seam gas content. A PLC controller controls the on-off and start-stop of each of an electromagnetic valve I, an electromagnetic valve II, an electromagnetic valve III, an electromagnetic valve IV, an electromagnetic valve V, an anti-explosive servo motor I, an anti-explosive servo motor II, andan anti-explosive high-speed motor in a control system; a central multifunctional processing display is used for estimating the lost gas quantity by sensing the measurement data of a liquid pressure sensor I, a liquid pressure sensor II, a gas pressure sensor, a temperature sensor, a methane concentration sensor, and an intelligent flowmeter; therewith, a coal sample is smashed through a preset program without opening a coal sample tank, and the gas dissipation is reduced; and finally, the system automatically corrects the gas content at each stage, and computes the final coal seam gas contentand issues a report. The one-key operation can be realized through a touch screen, the gas desorption quantity can be automatically metered, the measurement efficiency of the coal seam gas content isgreatly improved, and the determination result is accurate, the structure is simple, and the process operation is convenient.

The invention discloses a method for quantitively detecting methane-oxidizing bacterium. The method comprises the following steps: sampling; washing bacterium with PBS (Phosphate Buffer Solution) by shaking samples; designing and selecting a double-layer microfiltration membrane plate with specific pores according to the number of the samples; and filtering the bacteria, passing the membrane and fixing; and conducting fluorescent dyeing and result analysis. According to the fluorescent strength of samples on the microfiltration membrane plate, the number of the methane-oxidizing bacterium in soil samples or sediment samples can be quantitively calculated, so as to provide technical support for microorganism oil-gas exploration and microorganism oil-gas reservoir characteristics.

The invention relates to a computer vision-based system and a computer-vision based method for automatically measuring the viscosity of colored liquid. The system comprises a BS/U/M miniature suspended level viscometer fixed on a fixed bracket, a BS/U/M miniature suspended level viscometer connected with an exhaust system and a BS/U/M miniature suspended level viscometer connected with a computer vision system. The method comprises the following steps: (a) building a viscometer scale mark position template; (b) performing liquid level detection and tracking; (c) measuring the difference between the time when the liquid level passes through a lower scale mark and the time when the liquid level passes through an upper scale mark; and (d) performing the viscosity calculation to obtain the viscosity of liquid measured. The method performs automatic measurement, overcomes errors of manual measurement, and improves measurement accuracy and measurement efficiency.

A gas chromatography-quadrupole time-of-flight mass spectrometry/flame ionization detection method of flavor components in cigarette mainstream smoke comprises the following steps: collecting the flavor components in the cigarette mainstream smoke through adopting a Cambridge filter, extracting the flavor components with dichloromethane or n-hexane, taking the obtained extract liquid, analyzing the extract liquid, and allowing the obtained sample to undergo chromatographic column separation and to enter a quadrupole time-of-flight mass spectrometer (QTOF MS) and a flame ionization detector (FID) for determination in order to realize accurate qualitative diagnosis and quantification of the flavor components in cigarette mainstream smoke. The gas chromatography-quadrupole time-of-flight mass spectrometry/flame ionization detection method is applied to determination of the flavor components in cigarette mainstream smoke for the first time, and a QTOF MS spectrogram and an FID spectrogram can be simultaneously obtained only through one-shot sample introduction; and the components of a complex sample can be accurately characterized through the QTOF MS, a sample with very wide concentration range can be accurately quantified, and the retention times of the two spectrograms are completely same, so accurate qualitative diagnosis and quantification of the flavor components in cigarette mainstream smoke can be realized.

The invention discloses a qualitative and quantitative determination method of lactobacillus plantarum intestinal tract colonization. The qualitative determination method comprises the following steps of (1) conducting total deoxyribonucleic acid (DNA) extraction on excrement samples to be measured and pretreated; (2) conducting polymerase chain reaction (PCR) amplification of total DNA; (3) conducting PCR amplification of a V3 area; (4) conducting denaturing gel gradient electrophoresis on products after the PCR amplification of the V3 area and conducting gel imaging gel analysis; and (5) enabling a stripe of denaturing gradient gel obtained in the step (4) to be compared with a blank group and a comparing marking group. The quantitative determination method comprises the following steps of operating samples to be measured and lactobacillus plantarum series concentration bacterium powder according to the step (1) to the step (4), then building a standard curve for luminance of a stripe on series concentration lactobacillus plantarum denaturing gradient gel and contents, and finally calculating colonization lactobacillus plantarum quantity according to the standard curve.

The present invention relates to an optical fiber distribution type detecting method and the like equipped with a structure for enabling efficient measurement of a temperature distribution or strain distribution. This method regulates a modulation frequency and modulation index for probe light and pumping light opposingly incident on an object from a light source and a phase difference between the probe light and pumping light, thereby successively setting the length and location of search domains in a region to be measured. In particular, a detection process is executed while resetting the search domain length shorter at a predetermined interval of time or when an abnormality is detected. Thus partly changing the distance resolution for a specific region in the course of the detection process enables efficient measurement operations in a short time.

The invention discloses a method for measuring volatile components of coal and an application. The method comprises the following steps: 1) breaking test coal and sieving; 2) weighing a coal sample of set mass, heating and drying to constant mass, and putting the dried coal sample in a dry environment; 3) weighing a moisture content measuring test coal sample of set mass from the coal sample dried in the step 2), heating and drying the moisture content measuring test coal sample, and measuring the moisture content; 4) weighing a volatile component content measuring test coal sample of set mass from the coal sample dried in the step 2), and performing a volatile component content measuring test to measure the weight loss of the coal sample, wherein the ratio of the mass of the moisture content measuring test coal sample and the volatile component content measuring test coal to the sampled mass of the coal sample in the step 2) is 1:(3-20); and 5) calculating the volatile components in the coal sample according to the weight loss and moisture content of the coal sample.

The invention discloses a method for implementing oil-gas exploration and oil-gas reservoir characterization by using living bacteria exception and total bacteria exception of methylosinus trichosporium as indexes. The method comprises the following steps of: acquiring a sample above an oil-gas exploration and oil-gas reservoir characterization area according to the specific grid degree, samplingdepth and sampling quantity, and packing and storing the sample according to the specific conditions; simultaneously acquiring the living bacteria number and the total bacteria number of the methylosinus trichosporium in the sample by adopting a conventional microbe counting method, and drawing an equal living bacteria line and an equal total bacteria line on a map, a topographic map, a geologic map or a topographic-geologic map to obtain the living bacteria exception and the total bacteria exception; comprehensively comparing the living bacteria exception with the total bacteria exception; making a judgment and a prediction or providing an oil-gas reservoir exploitation scheme for the underground oil-gas resource condition of the oil-gas exploration and oil-gas reservoir characterizationarea according to the characteristics, similarities and differences of the living bacteria exception and the total bacteria exception by combining the results of geologic exploration, geochemical exploration and geophysical exploration.

The invention provides a rapid screening method of a coenzyme high-yielding Q10 strain, and relates to the coenzyme Q10. The method comprise: (1) extracting coenzyme Q10 samples from a broth by using supersonic wave; (2) primarily screening high-content coenzyme Q10 strains by using a microplate reader; and (3) secondarily screening by using HPLC. The method is improved on the basis of a conventional UV spectrophotometry, the coenzyme Q10 samples can be obtained through an ultrasonic crushing method for the strain broth, the coenzyme Q10 samples are converted into reduced-form coenzyme Q10 by using a reducing agent, the reduced-form coenzyme Q10 is used as a contrast to eliminate errors caused by proteins, nucleic acids and other impurities in the extracted liquid, and meanwhile the microplate reader is used for rapid detection of the coenzyme Q10 samples in batches, thereby both improving determination efficiency and guaranteeing reliability of the method. Finally, the HPLC method is used for secondary screening of few primarily screened high-yield strains to obtain the high-yielding coenzyme Q10 strain, thereby greatly shortening a breeding period and increasing efficiency of screening the high-yielding coenzyme Q10 strain.

The invention discloses a fan performance determination method based on a standard wind speed-power curve. The method comprises the following steps: (S1) multiple sets of wind speed-output power data of multiple different fans are acquired; (S2) wind speed-power curves of all the fans are built according to the multiple sets of wind speed-output power data; (S3) the wind speed-power curves of all the fans are formed to the standard wind speed-power curve; and (S4) the performance determination of all the fans is realized according to the standard wind speed-power curve. As the wind speed-power curves of all the fans are formed to the standard wind speed-power curve, the method can realize the unified determination and comparison of performances of the fans with different brands and model numbers so as to facilitate a generator set to obtain the maximum power generation efficiency.

The invention discloses a method for measuring intrinsic viscosity of a polymer. The method for measuring the intrinsic viscosity of the polymer comprises the following steps of: preparing a polymer solution and calculating an average value t0 of solvent flowing time; calculating an average value t1 of polymer solution flowing time; calculating an average value t2 of the polymer solution flowing time; measuring the flowing time t3, t4 and t5 of the polymer solution at the concentration of 1/2C0, 1/3C0 and 1/4C0 respectively; and calculating an intercept on a longitudinal coordinate, and calculating the intrinsic viscosity of the polymer according to an intrinsic viscosity calculation formula. By the method, the intrinsic viscosity of the polymer can be measured effectively; and the method is high in measuring precision and high in measuring efficiency, and the measuring cost of the intrinsic viscosity of the polymer is reduced.

The invention discloses a clamp for bone fracture connecting plate bending intensity and equivalent bending rigidity determination and a method, and belongs to the technical field of bone fracture connecting plate quality inspection. The clamp comprises an upper clamp and a lower clamp which are used in a mutually matched way, wherein the upper clamp comprises an upper clamp body; two downwards projected inner side roller shafts are arranged on the lower surface of the upper clamp body; the lower surface of each inner side roller shaft is an arc-shaped surface; the lower clamp comprises a lower clamp body; two upwards projected outer side roller shafts are arranged on the upper surface of the lower clamp body; the upper surface of each outer side roller shaft is an arc-shaped surface; three displacement sensors are arranged between the two outer side roller shafts in parallel; the displacement sensors positioned at the two sides correspond to the two inner side roller shafts. The invention also discloses a method for determining the bone fracture connecting plate by using the clamp. The clamp and the method have the advantages that the test efficiency and the test result accuracy are improved; the test cost is reduced.

The invention discloses a rapid determination method for ash content in a lignocellulosic biomass fuel. The method comprises the following steps: firing a measured biomass fuel sample of which the mass is 0.50-075g at the ashing temperature of 575-600 DEG C for 40-50 minutes, recording the mass of the measured biomass fuel sample before and after firing, so as to calculate the air-dried ash content in the measured biomass fuel. By adopting the method, the ash content of the biomass fuel can be quickly and efficiently determined, and the method has the advantages of high measurement result accuracy and low implementation cost. The invention also discloses a determination device for the ash content in the lignocellulosic biomass fuel by using the rapid determination method.

The invention discloses a lipidomics analysis method for toxoplasma gondii based on high performance liquid chromatography tandem high-resolution mass spectrum and application. The lipidomics analysismethod comprises the following steps: using a chloroform-methyl alcohol-aqueous solution for suspending a to-be-detected toxoplasma gondii sample and then performing ultrasonic disruption; centrifuging extracting solution, performing nitrogen blowing and re-dissolving with methyl alcohol, and then filtering and feeding the sample; using a liquid phase C18 chromatographic column for separating; utilizing mobile phases A and B composed of acetonitrile, isopropanol, ammonium acetate, methyl alcohol and water to elute, and then performing detecting in a full-scan manner respectively under positive and negative ion modes using a QTOF high-resolution mass spectrometer; and screening and identifying the detected data according to the chemical formula of target lipid molecules. The method has theadvantages of sensitivity and accuracy, and being independent of standard compound, and can be efficiently used for lipidomics analysis of the toxoplasma gondii.

The invention provides a lipoprotein cholesterol determination reagent and a kit, belonging to the technical field of biochemical detection. The reagent comprises poly-alpha-olefin, cholesterol oxidase and reaction promoter, and further comprises cholesterol esterase, peroxidase, catalase, catalase inhibitor, surface active agent, emulsifier, polyanion, divalent cation, stabilizer, anti-interference agent, color developing agent and preservative. The reagent and the kit provided by the invention can be used for full-automatic biochemical analyzer detection, are simple and convenient to operate, can improve detection efficiency and accuracy and can reduce detection cost.