4 results about "Chicken sample" patented technology

The application provides a SNP molecular marker and a breeding application method which are significantly related to the content of chicken meat flavor substance (E, E)-2,4-decadienal. The SNP molecular marker is rs316165567, contains a nucleotide sequence with a polymorphism of C / A at 2,519,798 bp of chicken chromosome 14, and when the genotype of the site is AA, the content of (E, E)-2,4-decadienal of the chicken meat sample is higher. The established molecular assisted breeding technology can early select the high-quality chicken flavor quality traits by determining the genotype of the SNP site of the measured chicken, can improve the content of (E, E)-2,4-decadienal of the chicken breast, and has great industrial application value and economic and scientific research value.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker remarkably related to the content of a chicken flavor substance (E, E)-2, 4-decadienal and a breeding application method. The SNP molecular marker is rs316165567 and contains nucleotide sequences with C / A polymorphism at 2nd, 519th and 798bp positions on a chicken chromosome 14, and when the genotype of the site is AA, the content of (E, E)-2, 4-decadienal of a corresponding chicken sample is high. According to the established molecular assisted breeding technology, the flavor quality character of the high-quality broiler chicken is selected in an early stage by determining the genotype of the SNP site of the chicken to be detected, the content of the chicken breast muscle (E, E)-2, 4-decadienal can be increased, and the molecular assisted breeding technology has great industrial application value and economic and scientific research value.

The invention discloses a zhou chicken molecular marker genotype and phenotype correlation analysis and breeding method. The method comprises the following steps: firstly, collecting zhou chicken samples, recording growth, slaughter and reproduction phenotype indexes, extracting genome DNA, screening specific SNP markers on GH, IGF-1 and PRL genes, judging genotypes through PCR amplification and Sanger sequencing, and screening AA (GH), GG (IGF-1) and TT (PRL) dominant genotypes through GLM model correlation analysis; and screening a core parent based on the dominant genotype, and carrying out artificial hybridization, hatching egg incubation, chick breeding, offspring genotype and phenotype double screening and multi-generation breeding to obtain an excellent new strain. According to the new strain, the weight of the 12-week-old cocks is larger than or equal to 1650 g, the weight of the hens is larger than or equal to 1300 g, the slaughter rate is larger than or equal to 88%, the first laying day age of the hens is smaller than or equal to 140 d, the annual egg yield is larger than or equal to 200, the growth speed and the annual egg yield are increased by 25% and 18% respectively compared with a traditional variety, the problems that traditional breeding is long in period and low in accuracy are solved, and accurate and efficient breeding is achieved.

The invention relates to the technical field of food detection, in particular to a method for detecting volatile compounds in chicken breast of white feather chickens by GC-IMS (gas chromatography-ion mobility spectrometry), which comprises the following steps: incubating a chicken sample for 15 minutes under the conditions of 500r / min and 60 DEG C, and then detecting by a gas chromatography-ion mobility spectrometer. Wherein the gas chromatography conditions are as follows: the temperature of a chromatographic column is 60 DEG C, the carrier gas is high-purity helium, the flow is gradually increased from 2.00 mL / min to 100.00 mL / min by adopting a gradient program, and the total operation time is 30 minutes; ion mobility spectrometry conditions include that a tritium ionization source is adopted, the length of a migration tube is 53 mm, the electric field intensity is 500 V / cm, drift gas is high-purity nitrogen, the flow velocity is 150 mL / min, and a positive ion mode is adopted. The method does not need complex pretreatment, has the advantages of high sensitivity, high analysis speed, high separation capacity and the like, can accurately detect 45 volatile flavor substances such as aldehydes, alcohols, ketones, esters and the like in chicken, and provides reliable technical support for chicken quality evaluation and product development.