34 results about "Chicken sample" patented technology

The invention discloses a method for using an electronic nose to detect the freshness of chicken. The invention aims at providing a method which uses the electronic nose to detect the freshness of chicken, has the advantages of rapidity and simpleness, easy use and objective and reliable detection result, and can reduce the impact of storing conditions to the detection result. The method comprises the following steps: a chicken sample to be detected is put into a sealed container and then heated in water bath with thermostatic control; the heated chicken sample is put into a test room, and then smell sent by the sample is detected by a gas sensor array which consists of four gas sensors of different types; the data which is aquired by the gas sensor array is collected and processed; and then the freshness of chicken is judged according to the processing result of the data. The detection method uses an electronic nose which consists of four gas sensors of different types to detect the chicken sample, and has the advantages of objective, accurate, and fast detection result, good repeatability, simple analysis process and small using amount of sensors, and reduces the extraction of eigenvalues.

The invention relates to a high performance liquid chromatography method for jointly, rapidly and accurately detecting the contents of melanin and inosinic acid in chicken of silky fowl. The method comprises the following steps of: (1) carrying out protease oscillating enzymolysis on a chicken sample for 24 hours, centrifuging to obtain liquid supernatant which serves as inosinic acid to-be-detected solution; (2) orderly washing precipitate which is subjected to enzymolysis and centrifugation with diethyl ether, absolute ethyl alcohol and ultrapure water, removing lipid and impurities, so as to obtain a melanin crude product, oxidizing the crude product into 2, 3, 5-tricarboxylic acid pyrrole under alkaline condition, extracting with diethyl ether, and drying; and (3) dissolving the dry crystal with the to-be-detected inosinic acid solution, loading and detecting the sample on a high performance liquid chromatograph, and quantifying by an external standard method. The high performance liquid chromatography method only needs little sample to simultaneously detect the contents of the melanin and inosinic acid in the chicken sample of silky fowl, moreover, the method is simple in experimental operation steps, saves detection cost, and is beneficial for acceleration of screening excellent silky fowl breed (series) or combination with both outstanding functions and flavor substances.

The invention relates to an indirect chemiluminescent enzyme linked immunosorbent assay (ELISA) kit for detecting residual ractopamine by using a monoclonal antibody, which comprises an ELISA plate with each pore coated with a coating antigen prepared by coupling ractopamine and bovine serum albumin, a ractopamine series standard solution, an ELISA goat-anti-mouse antibody solution, a ractopamine antibody solution, a luminescent solution, a washing solution, a coating solution and a sealing solution. The chemiluminescent ELISA kit carries out chemiluminescent ELISA by using the monoclonal antibody, wherein IC50=0.6 ng/ml. The lowest limit of detection in a chicken sample is 0.10 ng/ml, the coefficient of variation among batches and in a batch is smaller than 13%, and the recovery rate is 88-114%. In the invention, a new system for detecting the residual ractopamine is created by applying the monoclonal antibody and combining chemiluminescence with indirect ELISA, has higher sensitivity and specificity compared with the common ELISA and can play important role in the detection of the residual ractopamine in animal foods (such as milk, animal tissues and urine sample).

The invention discloses a PSE-like chicken judging method based on near infrared spectroscopy in the technical field of food detection. The method comprises the following steps: collecting the near infrared spectrum of a chicken sample, and measuring the pH value after 24h since slaughter; preprocessing spectral data to obtain a target matrix, and dividing the matrix into a calibration set and a prediction set; and establishing a prediction model on the basis of the samples in the calibration set and corresponding spectral data and the measured pH value after 24h since slaughter, and evaluating the prediction accuracy of the model. The method disclosed by the invention is simple and easy and quick in operation; and without special pretreatment on the sample, the method is widely applicable to the chilled products sold on the market as well as the forefront of industrial production.

The invention relates to the field of food science and animal husbandry, in particular to a comprehensive evaluation method for Xueshan chicken quality. The method includes: determining the following 7 meat quality evaluation indexes of a chicken sample, i.e. ATP, IMP, the pH value 1h after slaughter, water-holding capacity, shear force, flesh color and IMF, conversing the measured data average value of each meat quality index into an index value of corresponding grade, bringing the index values into a formula, calculating the proper selection index MQI value of meat quality traits, and determining the chicken quality according to the MQI value score. The method selects 7 main meat quality evaluation indexes, standardizes the determination method, and therefore can acquire more accurate and reliable experimental data; through correlation analysis of all the evaluation indexes, the interrelation among the indexes is determined, thus providing reference for establishment of a unified evaluation method and standard.

The invention provides an amikacin and kanamycin two-in-one fast detection enzyme linked immunosorbent assay kit, which comprises an enzyme labeling plate, an enzyme labeling antibody, a amikacin standard product, substrate color developing liquid, terminating liquid, washing liquid and an amikacin antibody, wherein amikacin hapten-protein conjugates are coated on the enzyme labeling plate. The kit disclosed by the invention can be used for qualitative and quantitative detection of residue of amikacin and kanamycin in egg and chicken samples. According to the method for detecting amikacin and kanamycin in the egg and chicken sample, firstly, the egg and chicken sample to be tested is treated; then, the enzyme linked immunosorbent assay kit is used for detection; finally, a detection result is analyzed. The enzyme linked immunosorbent assay kit provided by the invention has the advantages that the structure is simple; the use is convenient; the carrying is convenient; the detection method is efficient, accurate, simple and convenient; the kit is suitable for the qualitative and quantitative detection of mass samples.

The invention discloses a method for detecting the freshness of the chicken by an electrochemical method. The method comprises the following steps: (1), washing chicken samples with different freshness values in deionized water, sucking the moisture, smashing the chicken samples, weighing the samples, adding a solvent, carrying out ultrasonic extraction, and carrying out filtering to obtain filterliquor; (2), dripping and coating the filter liquor on the surface of a glassy carbon electrode, carrying out drying, immersing the electrode into a buffer solution, forming a three-electrode systemby the immersed electrode, a platinum electrode and a silver/silver chloride electrode, and carrying out electrochemical response spectrum acquisition on the filter liquor by using a linear voltammetry scanning method; (3), carrying out electrochemical response spectrum acquisition on chicken samples with unknown freshness values according to the process in the step (2); and (4), carrying out dataprocessing on collected electrochemical response spectra, extracting feature values, comparing differences of feature values of the samples with the unknown freshness values with samples with the known freshness values, and determining freshness values of the chicken samples. The detecting method has advantages of simple operation, sensitive reaction, low instrument cost, high speed and high accuracy and the like.

The invention discloses a specific primer for detecting Salmonella pullorum, and a kit containing the specific primer and application thereof. According to the invention, the primer for specifically amplifying Salmonella pullorum is designed directed at a specific fragment of Salmonella pullorum; a PCR detection method capable of directly detecting Salmonella pullorum in feces, eggs and chicken samples is established; the method has good specificity, high sensitivity and short detection time and rapidly and accurately detect Salmonella pullorum; and with the method, amplification results of 22 common pathogenic Salmonella serotypes and 8 common non-Salmonella pathogenic bacteria are negative, and Escherichia coli can be well distinguished. The invention provides a rapid and effective technical means for the detection of Salmonella pullorum; and the method has the advantages of simple operation, low requirements on equipment and short detection time, and can meet the demand of most poultry breeding factories for rapid detection of Salmonella pullorum.

The invention discloses a method for rapidly detecting the content of lactic acid bacteria in chicken on line. The method comprises the following steps: acquiring a hyperspectral image of chicken samples in a calibration set, and pretreating the acquired hyperspectral image for recognition of a target region and extraction of an average spectral data of the hyperspectral image; and putting the extracted spectral data into a formula. According to the invention, 21 optimal wavelengths are extracted from 486 full-wave bands to eliminate a large amount of redundant information and extract useful information, so the calculation amount of data analysis is reduced; the precision of a partial least square model is improved; and the demands of meat production enterprises for large-scale on-line production are achieved. Compared with the prior art, the method provided by the invention has the following beneficial effects: a to-be-detected sample is not needed to be pretreated, and non-contact spectral scanning on the to-be-detected sample is performed without destructiveness; the method does not use any chemical agents, is green and saves cost; and the method is easy to operate, saves time,and can realize large-scale on-line detection of the content of the lactic acid bacteria in the chicken.

The invention relates to a trace element spectrum method for tracing a phoenix-tailed chicken, and belongs to the technical field of phoenix-tailed chicken breed and origin traceability. According tothe trace element spectrum method, the varieties and the contents of trace elements in the phoenix-tailed chicken and a white-feather chicken are determinated, and then multiple comparative analysis is performed to obtain the varieties of the trace elements having significant content differences in the phoenix-tailed chicken and the white-feather chicken; then, variables that are effective for variety discrimination are obtained through principal component analysis, system clustering analysis and discriminant analysis, a discriminant model is established, and the validity of the model is verified, so that a trace element spectrum having effective discriminative power for a phoenix-tailed chicken sample and applicable to phoenix-tailed chicken traceability is obtained. By the trace elementspectrum method, the trace element spectrum applicable to the phoenix-tailed chicken breed and origin traceability can be determined, and the phoenix-tailed chicken can be accurately, quickly and effectively discriminated through the discriminant analysis model and the contents of three elements in the sample, so that the trace element spectrum method can be applied to the traceability and the discrimination of the phoenix-tailed chicken very well.

The invention discloses a salmonella enteritidis bacteriophage SEP37, an electrochemical impedance spectroscopy sensor and a detection method thereof, wherein the preservation number of the salmonella enteritidis bacteriophage SEP37 is CCTCC NO: M 20211127. According to the invention, the sensor takes the bacteriophage as a biological recognition element and consists of a measuring device comprising three electrodes, a nitrogen supply device and an electrochemical analyzer, wherein the electrochemical analyzer is communicated with a superior computer; the bacteriophage SEP37 has the characteristic of high specificity; the electrochemical impedance spectroscopy sensor obtained by utilizing the bacteriophage SEP37 is a potential tool capable of quickly and accurately detecting salmonella in various samples; the detection method of the sensor has the characteristics of low detection limit, high specificity, good stability and high detection speed; and when the sensor is used for detecting labeled lake water, lettuce and chicken samples, the result shows that the sensor can quickly and accurately detect and quantify the concentration of bacteria in the samples.

The invention discloses a method for relative quantitative analysis of broiler chicken heat shock proteins HSPA5. The method includes the steps: firstly, extracting the broiler chicken heat shock proteins HSPA5; secondly, performing enzyme digestion on the broiler chicken heat shock proteins HSPA5 to obtain specific peptide fragments; thirdly, detecting parent and daughter ion pairs of the specific peptide fragments by a liquid chromatograph-mass spectrometer to obtain the total signal intensity of daughter ions of the specific peptide fragments, acquiring the signal intensity of the broiler chicken heat shock proteins HSPA5 according to the total signal intensity of the daughter ions of the specific peptide fragments, and relatively quantifying the broiler chicken heat shock proteins HSPA5 in different treated broiler chicken samples by comparing the signal intensity of the broiler chicken heat shock proteins HSPA5 in the different treated broiler chicken samples. Amino acid sequencesof three specific peptide fragments of the broiler chicken heat shock proteins HSPA5 are as shown in SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3. Compared with an antibody-based Western Blot method, therelative quantitative method is stronger in specificity, the cumbersome step of antibody preparation is omitted, and chicken-origin HSPA5 protein detection flux and efficiency are improved.

The invention provides a nicarbazin detection kit. The nicarbazin detection kit comprises a 96-hole elisa plate, an enzyme marker, a nicarbazin marker standard substance, a 20*concentrated washing solution, a 20*concentrated reconstitution fluid, a substrate solution A, a substrate solution B and a stop solution, wherein the 96-hole elisa plate is coated with a nicarbazin hapten carrier protein, the enzyme marker is a nicarbazin specific antibody marked by horse radish peroxidase. The invention also provides an application of the nicarbazin detection kit in detection of chicken samples. The nicarbazin detection kit adopts a one-step method to detect the nicarbazin marker, is high in detection sensitivity, good in accuracy and precision of a detection result, simple in structure, convenientto use, good in portability, low in requirement on detection personnel and suitable for rapid detection of large-batch samples on site.

The invention belongs to the technical field of trimethylamine detection, and particularly relates to application of headspace gas chromatography in determination of low-concentration trimethylamine in chicken. The headspace gas chromatography can be used for detecting the content of low-concentration trimethylamine in chicken, and is applied to detection of fresh meat, refrigerated meat and chicken samples stored for a long time. The detection method of the headspace gas chromatography comprises the following steps of preparing alkali liquor, a trichloroacetic acid solution, a trimethylaminestandard stock solution and a trimethylamine standard working solution, drawing a trimethylamine standard curve and detecting a sample extracting solution. By optimizing parameters such as alkali liquor concentration, headspace balance temperature and carrier gas flow in headspace gas chromatography, the peak shape of a detected substance is effectively improved, and the detection sensitivity andrepeatability are improved; the method is suitable for determining the content of trimethylamine in chicken, particularly has an accurate determination effect on the low-concentration content, is short in balance time and high in accuracy, and is suitable for detecting various samples such as raw fresh meat, refrigerated meat and chicken stored for a long time.

The invention discloses a method for quickly detecting a chicken enterobacteriaceae content in an online mode. The method comprises the steps of acquiring a hyperspectral image of a calibration set chicken sample, pretreating the acquired spectral image, then recognizing a target area and extracting average spectral data of the spectral image; substituting the extracted spectral data into a formula. According to the method disclosed by the invention, 17 optimal wavelengths are extracted from 484 full wavebands, so that a lot of redundant information is removed, useful information is extracted,and the calculated amount of data analysis is reduced; thus, accuracy of a partial least squares model is improved, and a large-scale online production requirement of a meat enterprise is achieved. Compared with the prior art, the method disclosed by the invention has the following beneficial effects that a sample to be detected is prevented from being pretreated, only non-contact spectrum scanning is performed on the sample, and no destructiveness is generated to the sample; no chemical reagent is used by the method disclosed by the invention, so that greenness is achieved, and cost is reduced; the method disclosed by the invention is easy to operate, can save time and can achieve large-scale online detection on the chicken enterobacteriaceae content.

The invention discloses a method for detecting indicative polychlorinated biphenyl in chicken, and belongs to the technical field of detection. Acetone is used as an extractant, an anhydrous magnesium sulfate and sodium acetate mixed extracting salt bag is used as extracting salt, and a N-propylethylenediamine, florisil and terminated octadecyl silica gel mixed purifying bag is used as an adsorbent, the indicative polychlorinated biphenyl in the chicken is extracted and purified, and GC-MS/MS is used for detection. The seven kinds of indicative polychlorinated biphenyls are within the linear range of 0-100 [mu]g/L, the correlation coefficients are all 0.9980 or above, the detection limit is 0.1-0.5 [mu]g/kg, the quantification limit is 0.3-1.5 [mu]g/kg, and the sensitivity is high. The method has the advantages of few pretreatment steps, elimination of a matrix effect by using a matrix correction curve, high instrument sensitivity, simplicity, accuracy, high efficiency and the like, and has a good application prospect in batch and rapid detection of the indicative polychlorinated biphenyl in the chicken sample.

The invention discloses a method and a system for detecting staphylococcus aureus in chicken. The method comprises the following steps: acquiring a sample hyperspectral image; selecting a spectral image of a characteristic wavelength based on the sample hyperspectral image, setting a gray threshold, and performing segmentation processing on the selected spectral image to obtain a chicken sample to-be-detected area and a staphylococcus aureus to-be-detected area; extracting hyperspectral data; after the chicken sample hyperspectral data and the staphylococcus aureus hyperspectral data are mixed, characteristic wavelengths are extracted; selecting chicken sample hyperspectral data corresponding to the extracted characteristic wavelength to train a support vector machine model to obtain a staphylococcus aureus detection model; and detecting the staphylococcus aureus in the chicken by using the staphylococcus aureus detection model. The method can be used for simply, quickly and nondestructively detecting the staphylococcus aureus in the chicken product so as to meet the requirements of quality detection in the processing and selling processes of the chicken product.