A high-performance liquid chromatography method for simultaneous detection of melanin and inosinic acid content in black-bone chicken

A technology of high performance liquid chromatography and melanin, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex and time-consuming operation, low recovery rate, and low separation degree of thin-layer chromatography, and achieve shortened detection time, The effect of less sample volume and sufficient sample separation
CN103308614BActive Publication Date: 2014-10-01HANGZHOU ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HANGZHOU ACAD OF AGRI SCI
Publication Date
2014-10-01

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Abstract

The invention relates to a high performance liquid chromatography method for jointly, rapidly and accurately detecting the contents of melanin and inosinic acid in chicken of silky fowl. The method comprises the following steps of: (1) carrying out protease oscillating enzymolysis on a chicken sample for 24 hours, centrifuging to obtain liquid supernatant which serves as inosinic acid to-be-detected solution; (2) orderly washing precipitate which is subjected to enzymolysis and centrifugation with diethyl ether, absolute ethyl alcohol and ultrapure water, removing lipid and impurities, so as to obtain a melanin crude product, oxidizing the crude product into 2, 3, 5-tricarboxylic acid pyrrole under alkaline condition, extracting with diethyl ether, and drying; and (3) dissolving the dry crystal with the to-be-detected inosinic acid solution, loading and detecting the sample on a high performance liquid chromatograph, and quantifying by an external standard method. The high performance liquid chromatography method only needs little sample to simultaneously detect the contents of the melanin and inosinic acid in the chicken sample of silky fowl, moreover, the method is simple in experimental operation steps, saves detection cost, and is beneficial for acceleration of screening excellent silky fowl breed (series) or combination with both outstanding functions and flavor substances.
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Description

technical field

[0001] The invention relates to a high-performance liquid chromatography method for combined, rapid and accurate detection of melanin and inosinic acid in black-bone chicken meat, belonging to the field of agricultural analytical chemistry. Background technique

[0002] my country is a big chicken raising country in the world. According to FAO statistics, in 2011, the number of chickens raised in the world was 19.94 billion, and the number of chickens raised in China reached 4.61 billion, accounting for 23.1%. Based on differences in regions and customs, local chicken breeds of various shapes are produced by folk selection, such as Beijing oil chicken, Huiyang bearded chicken, Qingyuan Ma chicken, Taihe silky chicken, etc., which have become precious inheritance left to us by our ancestors. resource. Among them, black-bone chicken is also called black-bone chicken, which has the characteristics of black skin, black bone, and black meat. Replenishes deficien...

Claims

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