234 results about "Inosinic acid" patented technology

The present invention provides flavored seasoning compositions containing low-salt substitutes that reduce the quantify of common salt used to flavor foods. More specifically, the compositions contain (a) a flavored seasoning mixture comprising flavoring agents, and flow agents, bulking agents, binding agents and / or preservatives, and (b) a salt substitute containing potassium chloride and bitterness inhibitors taurine and 5′-adenosinic acid, 5′-inosinic acid and / or 5′-guanylic acid. Furthermore, the present invention provides foods containing these compositions, methods of using these compositions and methods for preparing them.

The invention discloses a preparation method of a polyinosinic acid-polycytidylic acid lyophilized powder injection. The method comprises the preparation of a polyinosinic acid-polycytidylic acid solution and a preparation of a lyophilized powder injection thereof. According to the invention, proper amounts of polyinosinic acid and polycytidylic acid are respectively dissolved by using normal saline; the mixtures are mixed and stirred, such that the polyinosinic acid-polycytidylic acid solution is prepared; a lyophilization additive is added and well mixed; and filtering, sub-packaging, and lyophilization are carried out. The prepared injection is suitable for frost preservation under a temperature below 0 DEG C. Therefore, expiration date is postponed, oxidative degradation speed is reduced, toxic and side effect are reduced, and stability is improved.

A salt replacing composition for replacing sodium chloride in food contains a major amount of potassium chloride, in combination with ammonium chloride, sucrose, one or more of disodium inosinate and disodium guanylate, an organic acid, and a salt of glutamic acid. A reduced sodium chloride composition contains the salt replacing composition and sodium chloride. A food containing the salt replacing composition and a process for making the salt replacing composition.

The invention aims to provide veterinary polyinosinic powder which mainly comprises poly cytidine acid, poly inosinic acid and auxiliary material, is prepared through a series of processes, such as mixing, dissolving, granulating, drying, grinding and the like, and is mainly used to prevent and treat the virus diseases of the livestock. Through the clinical therapeutic effect test we find that the medicine is characterized by good therapeutic effect, little dosage and high efficiency in preventing and treating the virus diseases of the livestock, and is suitable to be popularized and applied in the livestock industry.

The invention discloses an antibiotic free, hormone free and harmful additive free biological mixed feed capable of improving quality of meat and suitable for feeding of various meat animals, and a production method thereof. The biological mixed feed comprises the following materials, by weight: 8-10% of biological fermentation cotton seed meal, 3-5% of polypeptide biological blood powder, 80-84% of mixed forage powder, 3-5% of feed additive and 1-5% of plant oil. According to exogenous enzyme and microbial fermentation, macro-molecule protein raw materials are decomposed into polypeptides and small peptides, so as to degrade anti-nutritional factors like non-starch polysaccharides in the forage and increase utilization rate of the forage; meanwhile, abundant B-group vitamin, free amino acid and organic acidity are generated during a fermentation process to promote deposition of flavor amino acid, inosinic acid and unsaturated fatty acid in muscle, so as to enhance quality of meat. Freshwater fish, fryers and growing and fattening pigs fed by the biological mixed feed for animals of the invention have characteristics of fast growth speed, and elastic, chewy, delicious and strongly fragrant fish meat, chicken and pork. The method of the invention is simple, requires no special equipment and is suitable for industrialized production.

The invention provides an edible seasoning and the preparation technique of the edible seasoning, in particular to a concentrated chicken powder and the preparation technique of the chicken powder. Materials used for producing the concentrated chicken powder are: fresh chicken dices, fresh chicken leaf fat, flavour development glutamate, yeast extractive, inosinic acid, guanylic acid, white granulated sugar, salt and monosodium glutamate. The preparation technique of the concentrated chicken powder is: pretreatment of raw materials, dosing, boiling the materials, flanging, drying, cooling, crushing and packing. The flavour development glutamate, the inosinic acid and the guanylic acid are urged to composite to generate multiplier effect of freshness during the technical process. Standard equilibrium is carried out during each time of composition as required by the technical process, so as to improve the taste of the chicken product with much higher corresponding indexes of freshness and flavor than those of ordinary chicken powder.

Methods for producing purine nucleosides, and purine nucleotides, such as inosine and 5′-inosinic acid are provided which include using a bacterium belonging to the genus Bacillus or to the genus Escherichia wherein the purine nucleoside productivity of said bacterium is enhanced by increasing an activity of the YdhL protein. Also disclosed is the amino acid sequence of the YdhL protein from Bacillus amyloliquefaciens and the gene encoding it.

The invention relates to a feed additive of aquaculture industry, in particular to a meat quality flavor modifying agent for grass carp or carp and a preparation method thereof. Each kilogram of modifying agent comprises the following components: 30 to 50g of seaweed extract, 10 to 20g of combination of inosinic acid and guanylic acid, 20 to 30g of carnitine, 15 to 30g of glycin, 10 to 20g of alanine and the balance of zeolite powder. The preparation method comprises the following steps: firstly, crushing solid materials in the seaweed extract, the combination of inosinic acid and guanylic acid, the carnitine, the glycin, the alanine and the zeolite powder materials and sieving the crushed solid materials through a sieve with 80 to 100 meshes for later use; and secondly, mixing and stirring the crushed solid materials in the proportion to ensure that the coefficient of variation CV of the uniformity is less than 5 percent to obtain the product. The modifying agent can effectively improve protein content, content of total amino acids, content of essential amino acids and content of delicious amino acid in muscles of the grass carp and the carp.

The invention provides Chinese herbal medicine additive for improving meat quality of java tilapia, containing by weight of: 22-30% of spatholobus stem, 30-40% of black bean, 10-15% of glycyrrhiza, 5-10% of honeysuckle, 5-10% of radix isatidis and 10-15% of hawthorn. The advantages of the invention comprise: (1) having good phagostimulant effect, (2) substantially reducing fat content and cholesterol level in muscles of java tilapia, substantially increasing flavor substance content of inosinic acid in muscles, improving muscles quality of java tilapia, (3), adding and using conveniently, and directly adding in commercial feedstuff, wherein the components can generates synergistic effect with other additive components in commercial feedstuff, and raise weighting rate and disease resistance of java tilapia, and (4) having stabile raw material resource, low price, simple production process and easy for industrialization production.

The invention belongs to the technical field of special aquaculture, and particularly relates to a special taming feed for mandarin fish, and a mating taming method. The special taming feed for mandarin fish is prepared by mixing the following raw materials in parts by weight: 70 parts of White fish powder, 10 parts of shrimp meal, 5 parts of chicken intestine, 2 parts of a compound feed attractant, 5 parts of cod-liver oil, 1 parts of decavitamin, 2 parts of compound mineral substances and 5 parts of an adhesive, wherein the compound feed attractant is cuttlefish cream and inosinic acid. The invention further provides a progressive mandarin fish taming method. The mandarin fish can be entirely fed with the special feed through taming, the culturing cost and morbidity of mandarin fish can be reduced, and the sustainable development of the mandarin fish aquaculture industry can be realized.

The invention discloses dorking feed and a preparation method thereof. The dorking feed can be prepared by crushing and uniformly mixing wheat bran, distillers dried grains with soluble, maize, soybean meal, cotton dregs, rapeseed meal and other raw materials; adding disodium 5'-inosinate, disodium 5'-guanylate, methionine, threonine and other nutrition agents for uniformly mixing and then modulating by steam to cure cornstarch to a certain extent; and granulating to obtain the finished product. By using the invention, the content of the disodium 5'-inosinate in chicken can be improved and the edible flavor of the dorking can be improved. In addition, the method is simple, safe and reliable and cannot influence the growing vigor and the raising effect of the dorking.

The invention relates to a microbe cultivated poultry meat optimization feedstuff additive, which is characterized in that the invention takes optimized and treated compound microbes cereal crop byproducts as main culture medium and is produced by two stages of liquid expansion and solid fermentation. All bacteria types are addible bacteria allowed in 658 bulletin launched by Department of Agriculture, the liquid compound cultivation method of bacteria can be used to cultivate acquired bacteria in the same liquid medium, then the product can contain rich immune polypeptide, antioxidants and various vitamins, trace element chelate compound, active fungoid amylose, saponin, triterpene saponin and other substances that clean the free radical in animal body and significantly increase fat growth and inosinic acid content of poultry and improve the meat quality. The product has no toxin and residue with convenience use, and is the new feedstuff additive with safety and high efficiency.

The invention relates to a method for preparing a yeast extract containing 4 to 30 percent of inosinic acid disodium salt and guanylic acid disodium (I+G), which comprises the following steps: a) providing a yeast cream containing 8 weight percent or more of ribonucleic acid; b) killing yeasts in the yeast cream; c) performing centrifugal separation on the yeast cream to obtain a supernatant; d) adding enzyme to the supernatant simultaneously to perform enzymolysis; e) performing heat treatment on the supernatant after the enzymolysis to inactivate the enzyme; and f) concentrating and drying the supernatant with inactivated enzyme to obtain the yeast extract containing 4 to 30 percent of the inosinic acid disodium salt and the guanylic acid disodium. The invention also relates to a yeast extract which is prepared by the method and contains high-content of the I+G. The yeast extract prepared by the method and containing the I+G is rich in disodium nucleotide, integrates the advantages of the yeast extract and disodium 5' ribonucleotide, and has the characteristic of savageness without additives.

The invention discloses feed additive which improves the flavor of sea water cultivating fish. The feed additive consists of four or more than four types in aspartic acid, glutamic acid, glycin, arginine, lycine and L-carnitine by proportion. 0.5 percent to 5 percent of the feed additive which improves the flavor of the seawater cultivating fish of the invention is added in seawater fish feed, so that the total contents of amino acid and flavor developing amino acid required in fish is obviously improved, the content of saturated fatty acid is obviously reduced and the contents of unsaturated fatty acid is obviously improved, wherein, EPA and DHA are obviously improved; the content of inosinic acid in the fish is obviously improved. The feed additive which improves the flavor of sea water cultivating fish of the invention can obviously improve the flavor of the seawater fish, such as large yellow croaker, weever, etc., improve the contents of the flavor developing amino acid and the inosinic acid in the fish, reduce the content of body fat, and improve the content of the unsaturated fatty acid. The additive is safe without public harm and residue; the seawater fish cultivated by the additive meet the green food sanitation requirements.

The invention relates to a freshness detecting method used for royal jelly. In the specific detecting method, the contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), single phosphoric acid adenosine (AMP), inosinic acid (IMP), hypoxanthine riboside (HxR), hypoxanthine (Hx), adenine and adenosine of the royal jelly are quantitatively determined through methods of liquid chromatography or ultraviolet spectrometry, and the like; the ratio (F value) between the accumulation amount of adenine, adenosine, HxR and Hx and the sum of the qualities of the eight substances (degradation products of ATP and nucleic acid)is calculated; the F value of the royal jelly is used for detecting the freshness of the royal jelly and judging the quality of the royal jelly. Compared with a present royal jelly quality detecting method, the freshness detecting method can judge the quality and the freshness of the royal jelly more accurately and sensitively, can improve the quality standard of the present royal jelly and effectively monitor the quality of the royal jelly.

The present invention provides a polynucleotide adjuvant composition and methods of use in eliciting an immune response, in particular a mucosal immune response. The present invention also provides an immunogenic composition comprising the polynucleotide adjuvant composition together with other immunogenic compositions such as an antigen (e.g., as in a vaccine). The present invention further contemplates methods of use of such adjuvant compositions, particularly in eliciting an immune response, in particular a mucosal immune response to an antigenic compound.

The invention provides a compound feed for increasing the growing speed and improving the carcass quality of table poultry and a preparation method thereof, which can be used for solving the problems of residual antibiotics and poor carcass quality existing in the conventional table poultry cultivation. By adding a perilla seed extract into daily ration of table poultry, the growing speed of table poultry can be increased remarkably, the immunity of table poultry can be enhanced, lipid metabolism of mechanism small table poultry is improved, muscle rate is increased, abdomen fat percentage is decreased, the non-oxidizability of each position of a carcass is enhanced, and the contents of total reducing sugar, inosinic acid and glutamic acid in legs and chest muscles are increased remarkably. The compound feed is economical, and has a scientific mixture ratio; and the preparation method is simple and practical, and high social value can be created.

The invention discloses a large yellow croaker feed additive for reducing body fat and promoting flavor, which is prepared by uniformly mixing the following raw materials in parts by weight: 2-3 parts of L-carnitine, 20-30 parts of betaine and 60-70 parts of conjugated linoleic acid. After a daily complete formula feed is added with the additive (accounting for 2-3wt% of the daily complete formula feed), the fat content of muscles of large yellow croakers can be significantly reduced, and the contents of flavor compounds (such as flavor amino acids, inosinic acids and trimethylamine oxides) in the muscles of large yellow croakers can be increased; and when the complete formula feed added with the additive is used for feeding large yellow croakers, the fed large yellow croakers are low in fat content, plump in muscle, good in texture, and high in flavor amino acid, inosinic acid and trimethylamine oxide contents of muscles. Thus, the large yellow croakers are enough in freshness, exquisite in meat quality, and good in taste, and can be comparable to wild large yellow croakers.

The invention discloses a fresh ossein condiment and a preparation method thereof. The condiment is prepared from the following raw materials in parts by weight: 200-210 parts of chicken bone meat, 100-110 parts of pig bone meat, 50-55 parts of cattle bone meat, 10-12 parts of scallion, 20-23 parts of ginger, 18-20 parts of garlic, 15-18 parts of inosinic acid, 1-2 parts of guanylic acid, 13-15 parts of methionine, 8-10 parts of glycine, 4-5 parts of a bone breaking agent, 8-10 parts of netmeg, 4-5 parts of clove, 10-12 parts of minced squid meat, 3-4 parts of kelp particles, 5-6 parts of coffee bean powder, 4-5 parts of mung bean juice, 6-7 parts of dry white fungus, 3-4 parts of red bell peppers, 3-5 parts of laver, 8-9 parts of melon juice, 15-18 parts of grapefruit juice, 1-2 parts of lophatherum herb, 1-2 parts of honeysuckle, 0.5-1 part of safflower seed, 1-2 parts of plantain herb, 1-2 parts of rhizoma alismatis leaves, an appropriate amount of water, and 30-40 parts of a nutrient addition agent. The chicken bone meat, the pig bone meat and the cattle bone meat are added to the condiment disclosed by the invention, so that the condiment is rich in nutrition, delicious in taste and novel in technology.

The present invention relates to a microorganism producing inosine, which is one of purine nucleoside, an important material for 5′-inosinic acid synthesis, and method for producing inosine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus producing inosine at high concentration by inactivating the gene encoding nucleoside hydrolase II and by enhancing the expression of the gene encoding 5′-nucleotidase, which still retains the characteristics of Corynebacterium ammoniagenes CJIP2401 (KCCM-10610).

The invention discloses an attractant for marine fish and a preparation method for the attractant. The attractant consists of the following components in percentage by weight: 0.5 to 15 percent of cysteine, 10 to 30 percent of glutamic acid, 10 to 30 percent of betaine, 10 to 40 percent of Chinese asafetida, 5 to 20 percent of inosinic acid and flour added till the whole weight percentage is 100 percent. The attractant for the marine fish is prepared according to the ingestion characteristic of the marine fish by adopting multiple amino acids and attractive additives; and the attractant can provide nutrient components required for growth of the marine fish, improve the ingestion amount and reduce the bait coefficient and the raising cost. In addition, the attractant also has the advantages of no toxic hazard, convenience for use and the like. Moreover, the preparation method is simple and convenient.

The invention discloses a technology for fabricating a natural nutritional flavoring agent by taking lentinus edodes stems as a raw material. The pure natural nutritional flavoring agent which contains lentinus edodes and yeast flavoring materials and nutrient ingredients and does not contain sodium salt is fabricated by taking the lentinus edodes stems as the raw material and with a yeast fermentation method. The technology comprises the steps of decomposing the cell walls of the lentinus edodes stems to facilitate release of flavoring materials and nutrient ingredients in the cells, and simultaneously, performing double enzymolysis to decompose protein and nucleic acid. The lentinus edodes stems are fermented by the yeast, cellulose is decomposed, 5'-inosinic acid and 5'-guanylic acid generated by the nucleic acid are degraded by an enzyme technology, L-glutamic acid and L-asparaginic acid generated by the protein are hydrolyzed, and the delicate flavor of the flavoring agent is greatly improved by the synergistic effect of the flavoring amino acid and the flavoring nucleotide. The flavoring agent prepared by the preparation method is rich in a plurality of bioactive substances such as polysaccharides, amino acids, nucleotides and hemiterpene edible and medicinal fungus, namely lentinus edodes and yeast, does not contain sodium salt and any chemical ingredient or toxic substance, and is safe and environmentally friendly.

The invention discloses a phagostimulant for fish larvae and juveniles of spotted maigre and a preparation method thereof. The phagostimulant comprises the following compositions in portion by weight: 0.05 to 0.1 portion of dimethyl-beta- propionic acid thetine, 0.1 to 0.2 portion of kanirin, 0.1 to 0.3 portion of glycine trimethylpropyl, 20 to 30 portions of inosinic acid, 25 to 35 portions of clamworm extracting solution, 1 to 5 portions of betaine, 10 to 15 portions of lactamine, 8 to 10 portions of glycin, 26 to 28 portions of internal organ powder of cuttlefish, and 1 to 3 portions of lycopene. The preparation method of the phagostimulant comprises the following steps: raw materials are weighed according to weight portions and are mixed, and then the clamworm extracting solution produced by the mixing extraction of alcohol is added into the mixture. The phagostimulant has the advantages that the phagostimulant has small using amount, apparent effect, steady sources, low price, and simple production technology, can effectively improve survival rate, reduce the waste of baits, can also improve the high temperature resistance and the anti-anoxia ability of fish, strengthen the endurance of the fish to the change of water environment, and can improve the meat quality of breed varieties.

The invention relates to a yeast seasoning which comprises the ingredients based by parts by weight as follows: 40-75 parts of powdery yeast zymolyte, 10-35 parts of flavor enhancer, 1-3 parts of powder glucose or white sugar, 1-3 parts of inosinic acid, 1-3 parts of guanylic acid, 1-5 parts of table salt, 0.5-5 parts of natural flavoring agents and 10-35 parts of maltodextrin. The yeast seasoning made by taking the yeast zymolyte as a main ingredient has the advantages of being excellent in color, aroma and taste which are ideal and needed by food, and convenient to use.

The invention relates to a chemical fertilizer specially used for rice. The chemical fertilizer is prepared from the following components in percentage by weight: 18-23 parts of urea, 15-20 parts of ammonium bicarbonate, 10-15 parts of potassium chloride, 15-20 parts of potassium sulphate, 5-8 parts of zinc sulfate, 5-8 parts of sodium borate, 2-4 parts of ammonium chloride, 2-4 parts of nitrohumic acid, 2-4 parts of calcium magnesium phosphate, 1-3 parts of disodium guanylate, 1-3 parts of disodium inosinic acid, 1-3 parts of silicate mineral powder and the balance of glycerinum. The chemical fertilizer specially used for the rice, which adopts the technical features, can be applicable to the growth of all stages of the rice by controlling different ratios of the chemical fertilizer to an additive in the components.

The invention discloses a method for quickly detecting a delicious substance inosinic acid in raw and fresh pork based on a Raman spectrum, and relates to the technical field of livestock meat quality and safety detection. The method comprises the following steps: 1, measuring the Raman spectrum of an inosinic acid standard product; 2, obtaining the Raman spectrum of a raw and fresh pork sample; 3, measuring the content of inosinic acid by a chemical method; 4, performing Raman spectrum correction; 5, preferably selecting an inosinic acid feature peak; 6, calculating a feature Raman peak area; 7, constructing a detection model for the content of inosinic acid. According to the method, the delicious substance inosinic acid in the raw and fresh pork can be quickly detected by a microscopic confocal Raman spectrum technology, so that an effective quick pork delicious substance inosinic acid detection method which is easy to operate, low in cost and short in time is searched; the detection method is simple, safe and efficient and can meet the industrial production demand and the requirement of a customer on the quality of a product; therefore, the evaluation requirement on the flavor quality of a pork product in an actual production process is met.

The invention discloses a high-quality broiler chicken inosinic acid and intramuscular fat content related gene polymerization breeding method comprising the following steps: respectively amplifying a gene by using three pairs of primers under the condition of PCR (Polymerase Chain Reaction) by taking two-continuous-generation high-quality broiler chicken genome DNAs (Deoxyribose Nucleic Acids) as templates; judging the sizes of amplification products of the first primer, the second primer and the third primer according to agarose gel electrophoresis, and then, screening the mutation of three alkali bases by using a DNA sequencing technology, wherein the three alkali bases include an exon 9 of an adenylosuccinate lyase gene, a non-coding region of a phosphoribosylglycineamide synthetase-5-aminoimidazole ribonucleotide synthetase-phosphoribosylglycineamide transmethylase gene 5' and an intron 2 of an adipocyte fatty acid binding protein gene; and then, carrying out gene typing and gene frequency analysis on SNPs (Single Nucleotide Polymorphisms) of the three loci by using polyacrylamide gel electrophoresis. By using the high-quality broiler chicken inosinic acid and intramuscular fat content related gene polymerization breeding method, the favorable polymerization of a protogene type can be realized, and meanwhile, the genetic mechanisms of the chicken meat quality, flavor and character can be searched more comprehensively.

The invention relates to feed additives in aquiculture, in particular to a quality improver for low-salt or freshwater aquiculture of Litopenaeus vannamei Boone and a preparation method thereof. one kilogram of the improver comprises the following components: 30-50g of freeze-dried green algae powder, 20-50g of krill powder, 200-300g of eucommia ulmoides powder, 10-20g of inosinic acid, 10-20g ofsodium glutamate and the balance of zeolite powder. By adoption of the quality improver, the content of bromophenol which is a seafood flavor substance in muscle of low-salt or freshwater cultivated Litopenaeus vannamei Boone can be effectively increased, the content of inosinic acid which is a main seafood flavor substance in muscle of Litopenaeus vannamei Boone can be effectively enhanced, the body color of Litopenaeus vannamei Boone can be effectively improved, the contents of protein and flavoring amino acid in muscle of Litopenaeus vannamei Boone can be effectively increased, the attraction effect of feed of Litopenaeus vannamei Boone can be promoted and the growth property of Litopenaeus vannamei Boone can be improved to a certain extent, and the taste and flavor of Litopenaeus vannamei Boone can be improved finally.