23 results about "Inosinic acid" patented technology

The present invention relates to a novel PTS transporter subunit EIIC variant and a method of producing 5′-inosinic acid using the same. The PTS transporter subunit EIIC variant is obtained by substituting one or more amino acids in the amino acid sequence constituting PTS transporter subunit EIIC to change the activity of the protein, and a recombinant microorganism comprising the PTS transporter subunit EIIC variant is capable of efficiently producing 5′-inosinic acid.

The invention discloses oyster powder and a preparation method thereof, and belongs to the technical field of seasonings. The preparation method comprises the following steps: selecting fresh and live oysters, cleaning shells, opening the shells, taking meat, washing, mixing the oyster meat with purified water, slowly cooking at low temperature, then boiling at high temperature, and filtering to obtain oyster juice; mixing the oyster juice with maltodextrin, edible salt, white sugar, monosodium glutamate, disodium 5 '-inosinate and disodium 5'-guanylate, and sequentially performing high-temperature sterilization, compound enzyme enzymolysis and enzyme deactivation; and conveying the powdery precursor raw material to a spray drying tower for drying (with a spiral cyclone guide plate and three-stage temperature control), and performing three-stage discharging after drying to obtain the oyster powder. The method solves the problems of insufficient delicate flavor, poor solubility and high hygroscopicity of the existing oyster powder, and the prepared oyster powder is strong in delicate flavor, good in fluidity and stable in quality, and meets the use and storage requirements of seasonings.

The invention discloses bionic shark fin flavor essence, a probiotic microcapsule, a preparation process of the bionic shark fin flavor essence and the probiotic microcapsule and an instant seasoning. The bionic shark fin flavor essence is prepared from the following components in percentage by weight: 0.5%-1.5% of L-alanine, 0.3%-1.0% of glycine, 0.05%-0.4% of disodium 5 '-inosinate, 0.05%-0.4% of disodium 5'-guanylate and 0.1%-0.8% of sodium succinate. 1%-5% of yeast extract, 2%-8% of mushroom mycelium fermentation liquor, 0.05%-0.3% of ginger oleoresin and 0.03%-0.25% of garlic oleoresin; 20%-55% of propylene glycol and 5%-15% of sorbitol; 0.2%-3% of probiotic microcapsules subjected to embedding treatment; and the balance of deionized water. According to the bionic shark fin flavor essence provided by the invention, through an elaborately designed plant base and fermentation source formula, the dependence of the traditional shark fin flavor on seafood extracts is completely avoided, the seafood allergen risk is completely eradicated, and the consumption requirements of special crowds are met.

The present disclosure relates to the technical field of medicine, and in particular to a pharmaceutical composition, preparation method and use thereof. The pharmaceutical composition includes a first active ingredient, a second active ingredient, and a pharmaceutically acceptable carrier or excipient. The first active ingredient is a microbial agent, including one or more of Staphylococcus aureus, Bordetella pertussis, diphtheria toxoid, tetanus toxoid, Salmonella typhi, and Salmonella paratyphi. The second active ingredient includes polyinosinic acid, polycytidylic acid, and vitamin. The pharmaceutical composition of the present disclosure pertains to an artificial active immunotherapy for tumors. It can “stimulate” the entire immune system, making the therapy of using bacteria to activate the human immune system to kill cancer cells highly stable and reliable. This composition can significantly save and prolong the lives of cancer patients while exhibiting extremely high safety, minimal toxic side effects, and low production costs.

This invention provides a kit for the simultaneous detection of nine flavor nucleotides in livestock and poultry products. The flavor nucleotides are cytidine monophosphate, uridine monophosphate, guanylic acid, inosine monophosphate, adenosine monophosphate, hypoxanthine, inosine, adenosine diphosphate, and adenosine triphosphate. The kit includes: a high-concentration extraction reagent, a standard curve solution, a high-concentration phosphate buffer solution, pH adjustment solution A, and pH adjustment solution B. The high-concentration extraction reagent is perchloric acid; the standard curve solution is a mixed solution of the nine flavor nucleotides; the high-concentration phosphate buffer solution is a solution of potassium dihydrogen phosphate and dipotassium hydrogen phosphate; pH adjustment solution A is sodium hydroxide; and pH adjustment solution B is phosphate. This invention also provides a method for the simultaneous detection of the nine flavor nucleotides using the above kit. This invention enables the simultaneous determination of the content of nine flavor nucleotides in livestock and poultry products. The method is simple, rapid, accurate, and sensitive, and can be used for batch determination, showing good prospects for practical application.

The present invention relates to a novel ABC transporter permease variant and a method for producing 5 '-inosinic acid using the same. The ABC transporter permease variant has a modified protein activity due to the substitution of one or more amino acids in the amino acid sequence constituting the ABC transporter permease, and thus a recombinant microorganism comprising the ABC transporter permease variant can efficiently produce 5 '-inosinic acid.

The application relates to a production method and application of combined fermentation of bupleurum and lonicera japonica residues by bacillus velezensis and pediococcus acidilactici, and belongs to the technical field of biotechnology. The bupleurum and lonicera japonica residues are treated by adopting a process of enzymolysis first and then fermentation, wherein the composite enzyme comprises cellulase, beta-glucanase, xylanase, beta-mannanase and phytase; and the fermentation bacteria comprise bacillus velezensis and pediococcus acidilactici. The fermented bupleurum and lonicera japonica residues provided by the application have a high release amount of active ingredients (chlorogenic acid, forsythoside and baicalin), and the addition ratio in broiler chickens is 2.0%-4.0%. The application effect includes improving growth performance, improving the levels of anti-inflammatory factor IL-10 and serum immunoglobulin IgM of the body, improving the muscle quality and flavor characteristics of the breast muscle, and improving the contents of inosinic acid, intramuscular protein, flavor amino acid and total amino acid. The product can not only improve the yield and quality of livestock products, but also relieve the pressure on the environment caused by solid waste residues, and has a good market prospect.

The application belongs to the technical field of crystallization, and relates to a crystallization method of inosine disodium phosphate with controllable crystal size. The method comprises the following steps: mixing inosine disodium phosphate crude product with water and adjusting pH to obtain inosine disodium phosphate crude product aqueous solution; under the condition of constant temperature stirring, adding anhydrous ethanol to the crystallization system until turbidity appears in the system, stopping the addition of ethanol, then adding inosine disodium phosphate / ethanol suspension liquid to carry out the first crystal growth; after the first crystal growth is completed, continue to add anhydrous ethanol, and at the same time, the temperature of the crystallization system is lowered, and the second crystal growth is carried out under stirring; after the second crystal growth is completed, solid-liquid separation and drying are carried out to obtain inosine disodium phosphate crystals. Through step-by-step crystal growth and regulation, the effective control of the crystal size is realized, the process is stable, the product quality is excellent, the obtained inosine disodium phosphate crystals have controllable particle size, good fluidity and high purity.

The present invention discloses a pharmaceutical composition, its manufacturing method, and its use, wherein the pharmaceutical composition comprises a first active ingredient, a second active ingredient, and a pharmaceutically acceptable carrier or excipient, the first active ingredient being a microbial agent containing one or more of Staphylococcus aureus, Bordetella pertussis, diphtheria toxoid, tetanus toxoid, Salmonella typhi, or Salmonella paratyphi, and the second active ingredient comprising polyinosinic acid, polycytidylic acid, and vitamins. The pharmaceutical composition is an artificial active immunotherapy for tumors, which, by "stimulating" the entire immune system, activates the human immune system using bacteria to kill cancer cells, making the treatment extremely stable and reliable, significantly saving and extending the lives of cancer patients, while simultaneously being extremely safe, having very few toxic side effects, and having low manufacturing costs.

The invention discloses a molecular marker related to a chicken inosinic acid content character and a breeding application method thereof, and belongs to the technical field of molecular breeding. The InDel molecular marker corresponds to a No. 5 chromosome 14, 447 and 547 bp (the genotype is CTGAAACTGAAA, CTGAAAC and CC) in the version sequence information of a chicken reference genome Galusgallus-6. 0 published in NCBI (National Center of Biotechnology Information). The basic group is a favorable genotype with a CTGAAACTGAAA genotype as a site, and the content of inosinic acid in an individual carrying the basic group is obviously higher than that of an individual carrying a CC genotype. The molecular marker is used for molecular-assisted breeding, screening of high inosinic acid individuals can be achieved in the early stage, the production cost is effectively reduced, the genetic improvement progress is promoted, and high economic benefits and application values are achieved.

The application discloses a molecular marker related to a chicken meat inosinic acid content character and a breeding application method thereof, and belongs to the technical field of molecular breeding. The InDel molecular marker corresponds to the sequence information of a chicken reference genome Gallus_gallus-6.0 version sequence information No. 5 chromosome 14,447,547 bp (genotype CTGAAACTGAAA, CTGAAAC, CC). Here, the base is the advantageous genotype of the CTGAAACTGAAA genotype of the site, and the inosinic acid content of the individual carrying the site is significantly higher than that of the individual carrying the CC genotype. Molecular assisted breeding using the molecular marker can realize screening of high inosinic acid individuals at an early stage, effectively reduce production costs, promote genetic improvement progress, and has high economic benefits and application value.

A comprehensive evaluation method for mutton quality belongs to the fields of food science and animal husbandry. This method includes: (1) determination of multi-dimensional indicators: measuring the meat color brightness L... * , redness a * Yellowness b * (1) Shear force, water loss rate, pH value at 45 minutes and 24 hours post-slaughter, muscle fiber diameter, density, total area, area and total number of individual fibers, marbling grade, and key metabolites such as inosinic acid and fatty acids; (2) Standardized scoring conversion: convert the 14 indicators into 1 to 5 points according to preset thresholds; (3) Comprehensive scoring calculation: T=∑Sᵢ(i=1~14); (4) Quality grade classification: Special grade (T≥65), Excellent grade (55≤T<65), Good grade (35≤T<55), Ordinary grade (15≤T<35), Poor grade (T<15). This invention realizes the multi-dimensional integrated evaluation of sensory, texture, physicochemical, microstructure, appearance and flavor quality, and solves the problems of single indicators, inconsistent dimensions and lack of unified standards across parts in existing methods, providing technical support for accurate grading of mutton, market pricing and breed selection.

The invention provides a broiler chicken raising method based on nutrition regulation and environmental stress induction, and belongs to the technical field of livestock breeding, and the broiler chicken raising method comprises the following steps: dividing a raising cycle into four stages of 1-14 days old, 15-28 days old, 29-35 days old and 36-45 days old according to time, and performing nutrition regulation and environmental induction on the four stages, feed containing flaxseed meal and fish oil is fed at the 29-35-day-old stage, choline and betaine are added, periodic mild cold stress treatment is combined, and unsaturated fatty acid is promoted to be deposited into muscles; 80% limited feeding is carried out at the 36-45-day-old stage, meanwhile, a yeast extract, L-glutamine and a flavor enhancer composed of betaine and allicin are supplemented, the content of flavor substances such as inosinic acid and glutamic acid is effectively increased, and the flavor of the broiler chickens is remarkably improved. Meanwhile, muscle fibers are refined and oxidative damage is relieved by reducing the feeding density, adjusting illumination, prolonging the free-ranging time and the like.

The present invention relates to novel phosphoenolpyruvate carboxylase variants and methods of producing 5'-inosinic acid using the same. Due to the substitution of one or more amino acids in the amino acid sequence constituting the phosphoenolpyruvate carboxylase, the phosphoenolpyruvate carboxylase variant has a modified protein activity, and thus a recombinant microorganism comprising the phosphoenolpyruvate carboxylase variant can efficiently produce 5'-inosinic acid.

The invention provides a phagostimulant composition as well as a preparation method and application thereof, and relates to the technical field of aquatic feed phagostimulants. Comprising the following components in parts by weight: 10 to 30 parts of alanyl glycine, 10 to 25 parts of trimethylamine oxide, 10 to 20 parts of a compound of disodium 5 '-inosinate and disodium 5'-guanylate, 40 to 80 parts of a hydrolyzed animal protein substrate, 5 to 15 parts of krill oil, 1 to 5 parts of dimethyl-beta-thiofuran, 2 to 5 parts of betaine and 1 to 3 parts of multivitamins. According to the present invention, by compounding the multiple components, the feeding behavior of the mandarin fish can be effectively guided, the digestion and absorption ability of the mandarin fish can be improved, the stress behaviors such as ammonia nitrogen stress can be relieved, and the physiological homeostasis and the appetite of the mandarin fish can be ensured.

The present application relates to a microorganism with reduced branched-chain amino acid transporter activity, and a method for producing 5'-inosinic acid using same.

The invention provides a sensor for detecting ATP (adenosine triphosphate) related substances and application of the sensor. The sensor for detecting ATP-related substances has, on the same support, at least one of a reaction unit (1) in which a combination of enzymes capable of converting ATP, ADP, and AMP into xanthine is held on a solid-phase carrier, and a reaction unit (2) in which a combination of enzymes capable of converting inosinic acid into xanthine is held on a solid-phase carrier. And a reaction unit (3) in which a combination of enzymes capable of converting inosine into xanthine is held on a solid-phase carrier, wherein the combination of enzymes in each reaction unit contains xanthine oxidase. According to the present invention, the ATP-related substance can be easily and quickly detected even at the food manufacturing site, and the freshness of fresh food such as aquatic products and meat can be evaluated. In addition, not only K value and Ki value can be measured, but also the total concentration of ATP, ADP, and AMP and the concentration of inosinic acid can be measured.

The invention provides a yeast hydrolysate as well as a preparation method and application thereof, the yeast hydrolysate comprises the following components in percentage by mass: 35-65% of true protein, aromatic amino acid and sulfur-containing amino acid, and the content ratio of the aromatic amino acid to the sulfur-containing amino acid is 2-5; the ratio of the content of the components with the weight-average molecular weight of 200-2000Da to the content of the components with the weight-average molecular weight of more than 2000Da is 4-10, and the ratio of the content of the components with the weight-average molecular weight of 200-2000Da to the content of the components with the weight-average molecular weight of less than 200Da is 4-9; the yeast hydrolysate further comprises disodium nucleotide, the disodium nucleotide comprises disodium inosinate, disodium guanylate, disodium cytidine monophosphate and disodium uridinemonophosphate, and the ratio of the mass sum of the disodium inosinate and the disodium guanylate to the mass sum of the disodium cytidine monophosphate and the disodium uridinemonophosphate to the disodium nucleotide is 1: (0.6-1.2): (1.6-2.2). The feed additive is beneficial for further improving the feed intake of the piglets and reducing the disease incidence of the piglets.

The application provides a kind of for hot processing meat product and its preparation method of inhibiting harm low sodium flavor salt, belong to food processing technical field. The application mixes edible salt, flavor enhancer (including inosinic acid and guanylic acid) and water, obtains mixed solution;The mixed solution is carried out spray drying, and the obtained granular material is annealed, to obtain flavor-enhanced salt compound. The flavor-enhanced salt compound of the application can effectively release components with hydroxyl radical scavenging activity during cooking, and improve local moisture distribution through its unique hygroscopicity, thereby significantly inhibiting the generation of heterocyclic amines, advanced glycation end products and dihydroxyphenylalanine. At the same time, the flavor-enhanced salt compound has fast ion release kinetics and flavor synergy effect, so that the salty taste perception and overall sensory acceptability of roast beef patties are maintained or even improved under the condition of reducing sodium salt addition to 22%.

The present disclosure relates to a microorganism producing 5'-inosinic acid and a method for producing 5'-inosinic acid using same.

This invention discloses a complete feed for improving the sweetness value of the longissimus dorsi muscle of Shawutou pigs and its formulation method. This invention uses barley, wheat, and other bulk raw materials as macronutrient sources to enhance sweetness, silage and stevia residue for feed appetite regulation, and γ-aminobutyric acid (GABA), various vitamins, and minerals as micronutrients to regulate body health and fat metabolism, resulting in a complete feed that improves the sweetness value of the longissimus dorsi muscle of Shawutou pigs. This feed formulation improves the growth performance and feed utilization of Shawutou pigs; optimizes carcass composition, reduces backfat thickness while maintaining ideal intramuscular fat content; and significantly increases the content of sweet amino acids (such as glycine, alanine, serine, and proline), umami amino acids (glutamic acid), and inosinic acid (IMP) in the longissimus dorsi muscle, thereby enhancing the perceived sweetness value of the muscle and providing consumers with distinctive Shawutou pork with a prominent sweetness profile, thus increasing its market value.