81 results about "Inosinic acid" patented technology

The invention provides bacillus with a knockout mdr gene and application of the bacillus with the knockout mdr gene, the bacillus with the knockout mdr gene is obtained by guiding a knockout vector into original bacillus to knock out the mdr gene, and the original bacillus is bacillus amyloliquefaciens XH7 or bacillus licheniformis XZ3; the amino acid sequence of the mdr gene encoded in bacillus amyloliquefaciens is as shown in SEQ ID NO. 1, and the amino acid sequence of the mdr gene encoded in bacillus licheniformis is as shown in SEQ ID NO. 2. The recombinant plasmid with the mdr gene knocked out is designed and synthesized for the first time, the recombinant plasmid is transferred into bacillus to promote production of inosinic acid, and a new strategy is provided for increasing the yield of inosinic acid. Compared with bacillus amyloliquefaciens XH7 or bacillus licheniformis XZ3, the inosinic acid yield of the recombinant bacillus XH7 mdr or XZ3 mdr constructed by the invention is at least increased by 75.47% or above.

The invention belongs to the technical field of pork flavor additives, and particularly relates to a precursor substance additive composition for adjusting pork flavor and a preparation method thereof. According to the precursor substance additive composition prepared by the preparation method disclosed by the invention, various effective components such as the folium cortex eucommiae extracting solution, the liquorice root and the hawthorn fruit are scientifically proportioned and are matched with the plant slow-release agent and the blender, so that the gastrointestinal function and the disease resistance of pigs are regulated through a synergistic effect, and the contents of meat flavor pre-substances such as linoleic acid and inosinic acid in pork are increased; the plant slow-release agent is prepared in an embedding emulsion mode, release of active ingredients can be controlled in the digestion process of pigs, lasting nutritional supplement is provided, the anti-oxidation and immune effects of plant extracts are enhanced, the palatability of the pig feed is improved through the blender, the taste is enhanced, meanwhile, the antibacterial effect is enhanced, intestinal health and digestion are promoted, and the effect of improving the immunity of the pigs is achieved. The obtained pork is fresh, tender and juicy, and meets the requirements of consumers for high quality of meat products.

The invention provides a compound feed for promoting beef cattle growth and improving meat quality and a production method thereof, and belongs to the technical field of feed preparation. The compound feed comprises the following components in parts by weight: 100 parts of basal feed, 7-10 parts of a functional additive and 0.3-0.5 part of disodium inosinate, and the functional additive comprises a postbiotic compound, a fatty acid regulating agent, a bacterium enzyme synergist and trace elements, wherein the metagen complex comprises a lactobacillus plantarum metagen extract and a bacillus coagulans metagen extract, the fatty acid regulating agent comprises conjugated linoleic acid glyceride and medium-chain fatty acid, and the bacterial enzyme synergist comprises beta-mannase and bacillus subtilis. According to the compound feed, the growth performance and the meat quality can be synchronously improved, and a reliable processing technical guarantee is provided for improving the growth performance and the meat quality of beef cattle.

A kit for detecting drug-resistant tuberculosis, includes: detection reagents, configured to perform quantitative detection on 17 serum metabolites of patients with tuberculosis, the 17 serum metabolites comprising: taurine, homocysteine, uric acid, ascorbic acid, suberylglycine, uridine, dopamine 4-sulfate, inosinic acid, glyceraldehyde phosphate, [3-methoxy-4-(phosphoryl)phenyl]carbonyl sulfonic acid, nuclomedone, n4-cyclopropyl-6-(2,3-dichlorophenyl)-1,2,3,4-tetrahydropyrimidine-tetrachlorophthalic anhydride, malotilate, fulvic acid, and L-neopterin.

The present invention relates to a novel PTS transporter subunit EIIC variant and a method of producing 5′-inosinic acid using the same. The PTS transporter subunit EIIC variant is obtained by substituting one or more amino acids in the amino acid sequence constituting PTS transporter subunit EIIC to change the activity of the protein, and a recombinant microorganism comprising the PTS transporter subunit EIIC variant is capable of efficiently producing 5′-inosinic acid.

The present invention relates to a novel phosphoenolpyruvate carboxylase variant and a method for producing 5 '-inosinic acid using the same. The phosphoenolpyruvate carboxylase variant has modified protein activity due to the substitution of one or more amino acids in the amino acid sequence constituting the phosphoenolpyruvate carboxylase, and thus the recombinant microorganism comprising the phosphoenolpyruvate carboxylase variant can efficiently produce 5 '-inosinic acid.

The present invention relates to an Escherichia coli that efficiently produces 5'-inosinic acid and its application, belonging to the field of bioengineering technology. In order to reduce the degradation of 5'-inosinic acid in Escherichia coli, four phosphatase encoding genes were knocked out using the CRISPR / Cas9 system, and it was found that knocking out the UMP phosphatase encoding gene nagD and the 5'-nucleotidase encoding gene ushA respectively can increase the production of 5'-inosinic acid. Subsequently, in order to inhibit the further conversion of 5'-inosinic acid into guanylate and adenylate, the 5'-inosinic acid dehydrogenase encoding gene guaB was knocked out alone. Finally, in order to enhance the supply and utilization of formyltetrahydrofolate required in the purine synthesis process, the promoter of the serine hydroxymethyltransferase encoding gene glyA was replaced with P tac The recombinant Escherichia coli produced 2113.12 mg / L of 5'-inosinic acid in 48 hours of fermentation, and no antibiotics were required during the fermentation process, indicating that the product has broad application prospects.

The invention belongs to the technical field of bioengineering, and discloses a preparation method of an engineered exosome with a better treatment effect, and the preparation method comprises the following steps: S1, stimulating mesenchymal stem cells by using 10 [mu] g / mL of polyinosinic acid cytidine monophosphate for 1 h, and removing the polyinosinic acid cytidine monophosphate; s2, introducing a DMEM / F12 culture medium containing 10% of fetal calf serum into the mesenchymal stem cells subjected to stimulation treatment; s3, putting the mesenchymal stem cells into a cell incubator, culturing for 24 hours, and separating and purifying the exosome by adopting a conventional method; according to the invention, stem cells are pretreated by polyinosinic acid cytidine monophosphate, the stem cells are induced to secrete exosomes rich in therapeutic factors, and advanced preparation and purification technologies are combined, so that the engineered exosomes which are stable in quality and have a better therapeutic effect on osteoarthritis are successfully prepared.

The invention discloses oyster powder and a preparation method thereof, and belongs to the technical field of seasonings. The preparation method comprises the following steps: selecting fresh and live oysters, cleaning shells, opening the shells, taking meat, washing, mixing the oyster meat with purified water, slowly cooking at low temperature, then boiling at high temperature, and filtering to obtain oyster juice; mixing the oyster juice with maltodextrin, edible salt, white sugar, monosodium glutamate, disodium 5 '-inosinate and disodium 5'-guanylate, and sequentially performing high-temperature sterilization, compound enzyme enzymolysis and enzyme deactivation; and conveying the powdery precursor raw material to a spray drying tower for drying (with a spiral cyclone guide plate and three-stage temperature control), and performing three-stage discharging after drying to obtain the oyster powder. The method solves the problems of insufficient delicate flavor, poor solubility and high hygroscopicity of the existing oyster powder, and the prepared oyster powder is strong in delicate flavor, good in fluidity and stable in quality, and meets the use and storage requirements of seasonings.

The invention provides a method for simultaneously determining various flavor nucleotides in a livestock and poultry meat product and application, and the method comprises the following steps: freeze-drying the livestock and poultry meat product, and then preparing the freeze-dried livestock and poultry meat product into a test solution; measuring the contents of CMP (cytidylic acid), ATP (adenosine triphosphate), GMP (guanine monophosphate), IMP (inosinic acid), ADP (adenosine diphosphate), Hy (hypoxanthine), AMP (adenosine monophosphate) and IN (inosine) in the test solution by adopting HPLC (high performance liquid chromatography); in HPLC, a mobile phase A is a phosphate buffer solution with the volume concentration of 95%, a mobile phase B is methanol with the volume concentration of 5%, gradient elution is carried out, and the gradient elution condition is 0-11 min, 99% phosphate buffer solution and 1% methanol; when 12 min, 80% phosphate buffer solution and 20% methanol are added, and the conditions are maintained for 6-7 min; the phosphate buffer solution is heated to 99% within 2 minutes, and then the temperature is kept for 8-10 minutes.

The invention discloses a novel feed added with a composite plant extract and application thereof, and belongs to the technical field of feed preparation, namely breeding. The novel feed is obtained by adding composite plant extracts thymol and cinnamyl aldehyde in a mass ratio of 2: 3 into a basic feed, verification is performed after the Xiaoshan chicken is fed, and it is found that compared with the basic feed, the novel feed can effectively promote growth of the Xiaoshan chicken, improve slaughtering performance of the Xiaoshan chicken, improve meat color and improve the quality of the Xiaoshan chicken. The market competitiveness of muscles is improved; meanwhile, the increase of the content of fatty acid and inosinic acid brings qualitative leap to the flavor of Xiaoshan chicken. The novel feed provided by the invention has a remarkable application prospect in the field of breeding, especially in the field of Xiaoshan chicken breeding.

The present invention relates to a novel PTS transporter subunit EIIC variant and a method of producing 5′-inosinic acid using the same. The PTS transporter subunit EIIC variant is obtained by substituting one or more amino acids in the amino acid sequence constituting PTS transporter subunit EIIC to change the activity of the protein, and a recombinant microorganism comprising the PTS transporter subunit EIIC variant is capable of efficiently producing 5′-inosinic acid.

The present invention relates to a novel mutant of phosphoenolpyruvate carboxylase and a method for producing 5'-inosinic acid using the same. In the phosphoenolpyruvate carboxylase mutant, one or more amino acids in the amino acid sequence constituting phosphoenolpyruvate carboxylase are substituted, resulting in a change in the activity of the protein, and a recombinant microorganism containing the same can efficiently produce 5'-inosinic acid.

The present invention relates to a novel PTS transporter subunit EIIC variant and a method of producing 5'-inosinic acid using the same. The PTS transporter subunit EIIC variant is obtained by substituting one or more amino acids in the amino acid sequence constituting PTS transporter subunit EIIC to change the activity of the protein, and a recombinant microorganism comprising the PTS transporter subunit EIIC variant is capable of efficiently producing 5'-inosinic acid.

The invention discloses bionic shark fin flavor essence, a probiotic microcapsule, a preparation process of the bionic shark fin flavor essence and the probiotic microcapsule and an instant seasoning. The bionic shark fin flavor essence is prepared from the following components in percentage by weight: 0.5%-1.5% of L-alanine, 0.3%-1.0% of glycine, 0.05%-0.4% of disodium 5 '-inosinate, 0.05%-0.4% of disodium 5'-guanylate and 0.1%-0.8% of sodium succinate. 1%-5% of yeast extract, 2%-8% of mushroom mycelium fermentation liquor, 0.05%-0.3% of ginger oleoresin and 0.03%-0.25% of garlic oleoresin; 20%-55% of propylene glycol and 5%-15% of sorbitol; 0.2%-3% of probiotic microcapsules subjected to embedding treatment; and the balance of deionized water. According to the bionic shark fin flavor essence provided by the invention, through an elaborately designed plant base and fermentation source formula, the dependence of the traditional shark fin flavor on seafood extracts is completely avoided, the seafood allergen risk is completely eradicated, and the consumption requirements of special crowds are met.

The present invention relates to a novel 5-dehydro-2-deoxygluconate kinase variant and a method for producing 5 '-inosinic acid using the same. According to the present invention, the 5-dehydro-2-deoxygluconate kinase variant has a modified protein activity due to the substitution of one or more amino acids in the amino acid sequence constituting a 5-dehydro-2-deoxygluconate kinase, and thus a recombinant microorganism comprising the 5-dehydro-2-deoxygluconate kinase variant can efficiently produce 5 '-inosinic acid.

The present invention discloses an SNP molecular marker related to the inosine monophosphate content of blunt snout bream and its application. Such SNP molecular markers are base mutations at the 141st bp, 185th bp, 223rd bp, and 250th bp of the blunt snout bream ampd1 gene fragment sequence. Among them, the base at the 141st bp is A or T, the base at the 185th bp is A or C, the base at the 223rd bp is A or G, and the base at the 250th bp is A or G. When the SNP molecular marker of the present invention is used for assisted breeding or for correlation analysis of the inosine monophosphate content of blunt snout bream, blunt snout bream with high inosine monophosphate content, that is, a homozygous genotype variety, can be quickly and conveniently screened out. This not only lays a solid foundation for marker-assisted breeding selection of blunt snout bream (such as hybrid blunt snout bream and blunt snout bream), but also can improve the quality of blunt snout bream and enhance the production economic benefits.

A set of serum metabolic biomarkers and detection kit for detecting drug-resistant tuberculosis are provided. The set of the serum metabolic biomarkers includes 17 serum metabolic biomarkers. The 17 serum metabolic biomarkers are verified to be associated with tuberculosis based on the level changes of these biomarkers. The 17 metabolites includes taurine, homocysteine, uric acid, ascorbic acid, suberylglycine, uridine, dopamine 4-sulfate, inosinic acid, glyceraldehyde phosphate, [3-methoxy-4-(phosphoryl)phenyl]carbonyl sulfonic acid, nuclomedone, n4-cyclopropyl-6-(2,3-dichlorophenyl)-1,2,3,4-tetrahydropyrimidine-2,4-diimine, 1-[2-chlorine-2-(2,4-dichlorophenyl)ethenyl]-1,2,4-triazole, tetrachloro-phthalic anhydride, malotilate, fulvic acid, and L-neopterin. The serum metabolic biomarkers and detection kit for detecting drug-resistant tuberculosis can assist doctors in accurately diagnosing the disease, which is of great significance for the diagnosis and mass screening for drug-resistant tuberculosis.

The invention provides a construction method and application of bacillus amyloliquefaciens capable of enhancing yhcA expression, and the construction method comprises the following steps: taking bacillus amyloliquefaciens XH7 genome DNA as a template, carrying out PCR amplification on a transport protein coding gene yhcA, and connecting a promoter and a terminator to obtain a target gene segment; respectively carrying out double enzyme digestion on a free plasmid vector and a target gene segment by adopting the restriction enzyme to obtain a linear plasmid segment and an enzyme digestion gene segment; connecting the enzyme-digested gene fragment and the linear plasmid fragment through DNA ligase to obtain a free expression vector; and finally, transferring the free expression vector into a competent cell of bacillus amyloliquefaciens XH7, and adding a resistance selection marker to obtain a positive transformant. The research proves that the enhanced expression of the transporter gene yhcA is also a very effective method for improving the inosinic acid yield of bacillus amyloliquefaciens.

The invention discloses a compound flavor enhancer for improving meat quality of turbots and application of the compound flavor enhancer. The compound flavor enhancer comprises glutamic acid, aspartic acid, succinic acid, inosinic acid and guanylic acid. The compound flavor enhancer is applied to cultivation of turbots, namely, the compound flavor enhancer is used as an additive component of feed, and traditional chilled fish cultivation is replaced by compound feed cultivation added with the compound flavor enhancer. The formula of the feed is scientific in design, nutritional requirements of the turbots can be accurately met, the breeding cost is effectively reduced, and the addition of the compound flavor enhancer can significantly improve the adverse effect on the meat quality of the turbots caused by replacing chilled fresh fish with the compound feed on the premise of not influencing the growth performance; therefore, the meat quality and flavor of the scophthalmus maximus cultured by the compound feed are close to or even reach the level of iced fresh fish culture.

The present disclosure relates to the technical field of medicine, and in particular to a pharmaceutical composition, preparation method and use thereof. The pharmaceutical composition includes a first active ingredient, a second active ingredient, and a pharmaceutically acceptable carrier or excipient. The first active ingredient is a microbial agent, including one or more of Staphylococcus aureus, Bordetella pertussis, diphtheria toxoid, tetanus toxoid, Salmonella typhi, and Salmonella paratyphi. The second active ingredient includes polyinosinic acid, polycytidylic acid, and vitamin. The pharmaceutical composition of the present disclosure pertains to an artificial active immunotherapy for tumors. It can “stimulate” the entire immune system, making the therapy of using bacteria to activate the human immune system to kill cancer cells highly stable and reliable. This composition can significantly save and prolong the lives of cancer patients while exhibiting extremely high safety, minimal toxic side effects, and low production costs.

The present invention relates to a novel PTS transporter subunit EIIC variant and a method for producing 5 '-inosinic acid using the same. The PTS transporter subunit EIIC variant has an altered protein activity by replacing at least one amino acid in the amino acid sequence constituting the PTS transporter subunit EIIC, and thus a recombinant microorganism comprising the PTS transporter subunit EIIC variant can efficiently produce 5 '-inosinic acid.

The present invention relates to a novel 5-dehydro-2-deoxygluconokinase variant and a method of producing 5′-inosinic acid using the same. The 5-dehydro-2-deoxygluconokinase variant is obtained by substituting one or more amino acids in the amino acid sequence constituting 5-dehydro-2-deoxygluconokinase to change the activity of the protein, and a recombinant microorganism comprising the 5-dehydro-2-deoxygluconokinase variant is capable of efficiently producing 5′-inosinic acid.

The present invention provides an ATP-related substance detection sensor as well as a use thereof, the ATP-related substance detection sensor comprising, on the same support: at least one of a reaction section 1 in which a combination of enzymes capable of converting ATP, ADP, and AMP to xanthine is held on a solid-phase carrier and a reaction section 2 in which a combination of enzymes capable of converting inosinic acid to xanthine is held on a solid-phase carrier; and a reaction section 3 in which a combination of enzymes capable of converting inosine to xanthine is held on a solid-phase carrier. The combination of enzymes in each of the reaction sections includes xanthine oxidase. The present invention allows easy and rapid detection of an ATP-related substance at places including food production sites, for evaluation of the freshness of perishable food such as seafood and meat. Furthermore, the present invention allows, not only the measurement of the K value and the Ki value, but also the measurement of the total concentration of ATP, ADP and AMP, as well as inosinic acid concentration.

The invention belongs to the technical field of feed processing, and particularly relates to a fresh azolla filliculoidas and whole-plant corn mixed feed and a preparation method thereof.The fresh azolla filliculoidas and whole-plant corn mixed feed is prepared from 1400-1600 kg of fresh azolla filliculoidas, 900-1100 kg of whole-plant corn, 550-650 kg of bran and an additive. The main performance is as follows: the content of inosinic acid and total amino acid of pork is increased, the content of crude fat is reduced, so that the quality of the pork is more delicious, deep research finds that the fresh azolla filliculoidas feed is produced by mixing fresh azolla filliculoidas and whole-plant corn, adding strains and carrying out mixed fermentation, and the quality of the pork can be effectively improved by adding the fresh azolla filliculoidas feed into basic ration; and reference and suggestions are provided for pig breeding.

This invention provides a kit for the simultaneous detection of nine flavor nucleotides in livestock and poultry products. The flavor nucleotides are cytidine monophosphate, uridine monophosphate, guanylic acid, inosine monophosphate, adenosine monophosphate, hypoxanthine, inosine, adenosine diphosphate, and adenosine triphosphate. The kit includes: a high-concentration extraction reagent, a standard curve solution, a high-concentration phosphate buffer solution, pH adjustment solution A, and pH adjustment solution B. The high-concentration extraction reagent is perchloric acid; the standard curve solution is a mixed solution of the nine flavor nucleotides; the high-concentration phosphate buffer solution is a solution of potassium dihydrogen phosphate and dipotassium hydrogen phosphate; pH adjustment solution A is sodium hydroxide; and pH adjustment solution B is phosphate. This invention also provides a method for the simultaneous detection of the nine flavor nucleotides using the above kit. This invention enables the simultaneous determination of the content of nine flavor nucleotides in livestock and poultry products. The method is simple, rapid, accurate, and sensitive, and can be used for batch determination, showing good prospects for practical application.

Provided is a fish in which accumulation of inosinic acid is enhanced or accelerated during ripening. In a fish of the present disclosure, the function of the ecto-5′-nucleotidase (nt5e) gene is lost.

The present invention relates to a novel ABC transporter permease variant and a method for producing 5 '-inosinic acid using the same. The ABC transporter permease variant has a modified protein activity due to the substitution of one or more amino acids in the amino acid sequence constituting the ABC transporter permease, and thus a recombinant microorganism comprising the ABC transporter permease variant can efficiently produce 5 '-inosinic acid.

The application relates to a production method and application of combined fermentation of bupleurum and lonicera japonica residues by bacillus velezensis and pediococcus acidilactici, and belongs to the technical field of biotechnology. The bupleurum and lonicera japonica residues are treated by adopting a process of enzymolysis first and then fermentation, wherein the composite enzyme comprises cellulase, beta-glucanase, xylanase, beta-mannanase and phytase; and the fermentation bacteria comprise bacillus velezensis and pediococcus acidilactici. The fermented bupleurum and lonicera japonica residues provided by the application have a high release amount of active ingredients (chlorogenic acid, forsythoside and baicalin), and the addition ratio in broiler chickens is 2.0%-4.0%. The application effect includes improving growth performance, improving the levels of anti-inflammatory factor IL-10 and serum immunoglobulin IgM of the body, improving the muscle quality and flavor characteristics of the breast muscle, and improving the contents of inosinic acid, intramuscular protein, flavor amino acid and total amino acid. The product can not only improve the yield and quality of livestock products, but also relieve the pressure on the environment caused by solid waste residues, and has a good market prospect.