231 results about "Inosinic acid" patented technology

The invention discloses an antibiotic free, hormone free and harmful additive free biological mixed feed capable of improving quality of meat and suitable for feeding of various meat animals, and a production method thereof. The biological mixed feed comprises the following materials, by weight: 8-10% of biological fermentation cotton seed meal, 3-5% of polypeptide biological blood powder, 80-84% of mixed forage powder, 3-5% of feed additive and 1-5% of plant oil. According to exogenous enzyme and microbial fermentation, macro-molecule protein raw materials are decomposed into polypeptides and small peptides, so as to degrade anti-nutritional factors like non-starch polysaccharides in the forage and increase utilization rate of the forage; meanwhile, abundant B-group vitamin, free amino acid and organic acidity are generated during a fermentation process to promote deposition of flavor amino acid, inosinic acid and unsaturated fatty acid in muscle, so as to enhance quality of meat. Freshwater fish, fryers and growing and fattening pigs fed by the biological mixed feed for animals of the invention have characteristics of fast growth speed, and elastic, chewy, delicious and strongly fragrant fish meat, chicken and pork. The method of the invention is simple, requires no special equipment and is suitable for industrialized production.

The invention provides an edible seasoning and the preparation technique of the edible seasoning, in particular to a concentrated chicken powder and the preparation technique of the chicken powder. Materials used for producing the concentrated chicken powder are: fresh chicken dices, fresh chicken leaf fat, flavour development glutamate, yeast extractive, inosinic acid, guanylic acid, white granulated sugar, salt and monosodium glutamate. The preparation technique of the concentrated chicken powder is: pretreatment of raw materials, dosing, boiling the materials, flanging, drying, cooling, crushing and packing. The flavour development glutamate, the inosinic acid and the guanylic acid are urged to composite to generate multiplier effect of freshness during the technical process. Standard equilibrium is carried out during each time of composition as required by the technical process, so as to improve the taste of the chicken product with much higher corresponding indexes of freshness and flavor than those of ordinary chicken powder.

Methods for producing purine nucleosides, and purine nucleotides, such as inosine and 5′-inosinic acid are provided which include using a bacterium belonging to the genus Bacillus or to the genus Escherichia wherein the purine nucleoside productivity of said bacterium is enhanced by increasing an activity of the YdhL protein. Also disclosed is the amino acid sequence of the YdhL protein from Bacillus amyloliquefaciens and the gene encoding it.

The invention relates to a feed additive of aquaculture industry, in particular to a meat quality flavor modifying agent for grass carp or carp and a preparation method thereof. Each kilogram of modifying agent comprises the following components: 30 to 50g of seaweed extract, 10 to 20g of combination of inosinic acid and guanylic acid, 20 to 30g of carnitine, 15 to 30g of glycin, 10 to 20g of alanine and the balance of zeolite powder. The preparation method comprises the following steps: firstly, crushing solid materials in the seaweed extract, the combination of inosinic acid and guanylic acid, the carnitine, the glycin, the alanine and the zeolite powder materials and sieving the crushed solid materials through a sieve with 80 to 100 meshes for later use; and secondly, mixing and stirring the crushed solid materials in the proportion to ensure that the coefficient of variation CV of the uniformity is less than 5 percent to obtain the product. The modifying agent can effectively improve protein content, content of total amino acids, content of essential amino acids and content of delicious amino acid in muscles of the grass carp and the carp.

The invention provides Chinese herbal medicine additive for improving meat quality of java tilapia, containing by weight of: 22-30% of spatholobus stem, 30-40% of black bean, 10-15% of glycyrrhiza, 5-10% of honeysuckle, 5-10% of radix isatidis and 10-15% of hawthorn. The advantages of the invention comprise: (1) having good phagostimulant effect, (2) substantially reducing fat content and cholesterol level in muscles of java tilapia, substantially increasing flavor substance content of inosinic acid in muscles, improving muscles quality of java tilapia, (3), adding and using conveniently, and directly adding in commercial feedstuff, wherein the components can generates synergistic effect with other additive components in commercial feedstuff, and raise weighting rate and disease resistance of java tilapia, and (4) having stabile raw material resource, low price, simple production process and easy for industrialization production.

The invention belongs to the technical field of special aquaculture, and particularly relates to a special taming feed for mandarin fish, and a mating taming method. The special taming feed for mandarin fish is prepared by mixing the following raw materials in parts by weight: 70 parts of White fish powder, 10 parts of shrimp meal, 5 parts of chicken intestine, 2 parts of a compound feed attractant, 5 parts of cod-liver oil, 1 parts of decavitamin, 2 parts of compound mineral substances and 5 parts of an adhesive, wherein the compound feed attractant is cuttlefish cream and inosinic acid. The invention further provides a progressive mandarin fish taming method. The mandarin fish can be entirely fed with the special feed through taming, the culturing cost and morbidity of mandarin fish can be reduced, and the sustainable development of the mandarin fish aquaculture industry can be realized.

The invention discloses feed additive which improves the flavor of sea water cultivating fish. The feed additive consists of four or more than four types in aspartic acid, glutamic acid, glycin, arginine, lycine and L-carnitine by proportion. 0.5 percent to 5 percent of the feed additive which improves the flavor of the seawater cultivating fish of the invention is added in seawater fish feed, so that the total contents of amino acid and flavor developing amino acid required in fish is obviously improved, the content of saturated fatty acid is obviously reduced and the contents of unsaturated fatty acid is obviously improved, wherein, EPA and DHA are obviously improved; the content of inosinic acid in the fish is obviously improved. The feed additive which improves the flavor of sea water cultivating fish of the invention can obviously improve the flavor of the seawater fish, such as large yellow croaker, weever, etc., improve the contents of the flavor developing amino acid and the inosinic acid in the fish, reduce the content of body fat, and improve the content of the unsaturated fatty acid. The additive is safe without public harm and residue; the seawater fish cultivated by the additive meet the green food sanitation requirements.

The invention relates to a freshness detecting method used for royal jelly. In the specific detecting method, the contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), single phosphoric acid adenosine (AMP), inosinic acid (IMP), hypoxanthine riboside (HxR), hypoxanthine (Hx), adenine and adenosine of the royal jelly are quantitatively determined through methods of liquid chromatography or ultraviolet spectrometry, and the like; the ratio (F value) between the accumulation amount of adenine, adenosine, HxR and Hx and the sum of the qualities of the eight substances (degradation products of ATP and nucleic acid)is calculated; the F value of the royal jelly is used for detecting the freshness of the royal jelly and judging the quality of the royal jelly. Compared with a present royal jelly quality detecting method, the freshness detecting method can judge the quality and the freshness of the royal jelly more accurately and sensitively, can improve the quality standard of the present royal jelly and effectively monitor the quality of the royal jelly.

The invention discloses a large yellow croaker feed additive for reducing body fat and promoting flavor, which is prepared by uniformly mixing the following raw materials in parts by weight: 2-3 parts of L-carnitine, 20-30 parts of betaine and 60-70 parts of conjugated linoleic acid. After a daily complete formula feed is added with the additive (accounting for 2-3wt% of the daily complete formula feed), the fat content of muscles of large yellow croakers can be significantly reduced, and the contents of flavor compounds (such as flavor amino acids, inosinic acids and trimethylamine oxides) in the muscles of large yellow croakers can be increased; and when the complete formula feed added with the additive is used for feeding large yellow croakers, the fed large yellow croakers are low in fat content, plump in muscle, good in texture, and high in flavor amino acid, inosinic acid and trimethylamine oxide contents of muscles. Thus, the large yellow croakers are enough in freshness, exquisite in meat quality, and good in taste, and can be comparable to wild large yellow croakers.

The invention discloses a fresh ossein condiment and a preparation method thereof. The condiment is prepared from the following raw materials in parts by weight: 200-210 parts of chicken bone meat, 100-110 parts of pig bone meat, 50-55 parts of cattle bone meat, 10-12 parts of scallion, 20-23 parts of ginger, 18-20 parts of garlic, 15-18 parts of inosinic acid, 1-2 parts of guanylic acid, 13-15 parts of methionine, 8-10 parts of glycine, 4-5 parts of a bone breaking agent, 8-10 parts of netmeg, 4-5 parts of clove, 10-12 parts of minced squid meat, 3-4 parts of kelp particles, 5-6 parts of coffee bean powder, 4-5 parts of mung bean juice, 6-7 parts of dry white fungus, 3-4 parts of red bell peppers, 3-5 parts of laver, 8-9 parts of melon juice, 15-18 parts of grapefruit juice, 1-2 parts of lophatherum herb, 1-2 parts of honeysuckle, 0.5-1 part of safflower seed, 1-2 parts of plantain herb, 1-2 parts of rhizoma alismatis leaves, an appropriate amount of water, and 30-40 parts of a nutrient addition agent. The chicken bone meat, the pig bone meat and the cattle bone meat are added to the condiment disclosed by the invention, so that the condiment is rich in nutrition, delicious in taste and novel in technology.

The present invention relates to a microorganism producing inosine, which is one of purine nucleoside, an important material for 5′-inosinic acid synthesis, and method for producing inosine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus producing inosine at high concentration by inactivating the gene encoding nucleoside hydrolase II and by enhancing the expression of the gene encoding 5′-nucleotidase, which still retains the characteristics of Corynebacterium ammoniagenes CJIP2401 (KCCM-10610).

The invention discloses an attractant for marine fish and a preparation method for the attractant. The attractant consists of the following components in percentage by weight: 0.5 to 15 percent of cysteine, 10 to 30 percent of glutamic acid, 10 to 30 percent of betaine, 10 to 40 percent of Chinese asafetida, 5 to 20 percent of inosinic acid and flour added till the whole weight percentage is 100 percent. The attractant for the marine fish is prepared according to the ingestion characteristic of the marine fish by adopting multiple amino acids and attractive additives; and the attractant can provide nutrient components required for growth of the marine fish, improve the ingestion amount and reduce the bait coefficient and the raising cost. In addition, the attractant also has the advantages of no toxic hazard, convenience for use and the like. Moreover, the preparation method is simple and convenient.

The invention discloses a phagostimulant for fish larvae and juveniles of spotted maigre and a preparation method thereof. The phagostimulant comprises the following compositions in portion by weight: 0.05 to 0.1 portion of dimethyl-beta- propionic acid thetine, 0.1 to 0.2 portion of kanirin, 0.1 to 0.3 portion of glycine trimethylpropyl, 20 to 30 portions of inosinic acid, 25 to 35 portions of clamworm extracting solution, 1 to 5 portions of betaine, 10 to 15 portions of lactamine, 8 to 10 portions of glycin, 26 to 28 portions of internal organ powder of cuttlefish, and 1 to 3 portions of lycopene. The preparation method of the phagostimulant comprises the following steps: raw materials are weighed according to weight portions and are mixed, and then the clamworm extracting solution produced by the mixing extraction of alcohol is added into the mixture. The phagostimulant has the advantages that the phagostimulant has small using amount, apparent effect, steady sources, low price, and simple production technology, can effectively improve survival rate, reduce the waste of baits, can also improve the high temperature resistance and the anti-anoxia ability of fish, strengthen the endurance of the fish to the change of water environment, and can improve the meat quality of breed varieties.

The invention relates to a chemical fertilizer specially used for rice. The chemical fertilizer is prepared from the following components in percentage by weight: 18-23 parts of urea, 15-20 parts of ammonium bicarbonate, 10-15 parts of potassium chloride, 15-20 parts of potassium sulphate, 5-8 parts of zinc sulfate, 5-8 parts of sodium borate, 2-4 parts of ammonium chloride, 2-4 parts of nitrohumic acid, 2-4 parts of calcium magnesium phosphate, 1-3 parts of disodium guanylate, 1-3 parts of disodium inosinic acid, 1-3 parts of silicate mineral powder and the balance of glycerinum. The chemical fertilizer specially used for the rice, which adopts the technical features, can be applicable to the growth of all stages of the rice by controlling different ratios of the chemical fertilizer to an additive in the components.

The invention discloses a high-quality broiler chicken inosinic acid and intramuscular fat content related gene polymerization breeding method comprising the following steps: respectively amplifying a gene by using three pairs of primers under the condition of PCR (Polymerase Chain Reaction) by taking two-continuous-generation high-quality broiler chicken genome DNAs (Deoxyribose Nucleic Acids) as templates; judging the sizes of amplification products of the first primer, the second primer and the third primer according to agarose gel electrophoresis, and then, screening the mutation of three alkali bases by using a DNA sequencing technology, wherein the three alkali bases include an exon 9 of an adenylosuccinate lyase gene, a non-coding region of a phosphoribosylglycineamide synthetase-5-aminoimidazole ribonucleotide synthetase-phosphoribosylglycineamide transmethylase gene 5' and an intron 2 of an adipocyte fatty acid binding protein gene; and then, carrying out gene typing and gene frequency analysis on SNPs (Single Nucleotide Polymorphisms) of the three loci by using polyacrylamide gel electrophoresis. By using the high-quality broiler chicken inosinic acid and intramuscular fat content related gene polymerization breeding method, the favorable polymerization of a protogene type can be realized, and meanwhile, the genetic mechanisms of the chicken meat quality, flavor and character can be searched more comprehensively.

The invention discloses an instant shark skin, which is produced by mixing, boiling and thickening the following raw materials with the following weight percents: 12 to 15 percent of fishy smell removed sharp skin, 3 to 5 percent of compounding material, and 80 to 85 percent of dripping seasoning. The compounding material is prepared by mushroom, black fungus, white fungus, veiled lady and dried scallop with a wet weight proportion of 3:3:6:6:2 after soaked. The dripping seasoning is prepared by the following dripping materials with the following weight percents and water: 10 to 15 percent of abalone juice, 0.3 to 0.5 percent of fresh chicken powder, 0.3 to 0.5 percent of white spirit, 0.1 to 0.3 percent of inosine monophosphate and guanosine monophosphate, 0.05 to 0.1 percent of salt, 0.01 to 0.05 percent of chicken extract, and 2 to 4 percent of starch. The invention has the advantages of convenient use, high nutritional value and good taste. The invention also discloses a preparation method of the instant sharp skin. The method has the advantage of good fishy smell removing effect; in addition, the compounding material and the dripping seasoning for the preparation of the sharp skin taste delicious and are rich in various nutrients.

The invention discloses a complex seasoning of stock of spareribs, which is mainly prepared by edible salt, monosodium glutamate, crystal sugar, flavoring essence of spareribs, sodium guanylate, disodium inosinate, star anise and hydrolyzed vegetable protein according to a certain weight proportion; the invention has superior sorting, unique formulation and fresh taste and can be used for seasoning of foods with convenience, fastness and good effect.