54 results about "Pork sample" patented technology

The invention discloses an SNP (single nucleotide polymorphism) marker for identifying MC1R (melanocortin receptor 1) genes of pig breeds and colors of the pig breeds and further discloses an SNP marker primer for identifying the MC1R genes of the pig breeds and the colors of the pig breeds as well as an SNP marker method for identifying the MC1R genes of the pig breeds and the colors of the pig breeds. The molecular genetic marker is not limited by ages, sexes and the like of pigs, can be applied to early breeding of pure breeds of local black hogs and can further be used for performing pig breed and color identification and genetic relationship deduction on pork samples from unknown sources. A screening method is accurate, operation is simple, the cost is low, and the efficiency is high.

The invention discloses a method for quickly detecting a delicious substance inosinic acid in raw and fresh pork based on a Raman spectrum, and relates to the technical field of livestock meat quality and safety detection. The method comprises the following steps: 1, measuring the Raman spectrum of an inosinic acid standard product; 2, obtaining the Raman spectrum of a raw and fresh pork sample; 3, measuring the content of inosinic acid by a chemical method; 4, performing Raman spectrum correction; 5, preferably selecting an inosinic acid feature peak; 6, calculating a feature Raman peak area; 7, constructing a detection model for the content of inosinic acid. According to the method, the delicious substance inosinic acid in the raw and fresh pork can be quickly detected by a microscopic confocal Raman spectrum technology, so that an effective quick pork delicious substance inosinic acid detection method which is easy to operate, low in cost and short in time is searched; the detection method is simple, safe and efficient and can meet the industrial production demand and the requirement of a customer on the quality of a product; therefore, the evaluation requirement on the flavor quality of a pork product in an actual production process is met.

The invention discloses a device for detecting pork heavy metal distribution through tunable resonance laser-induced breakdown spectroscopy. According to the device, a pork sample subjected to dryingand tabletting is subjected to ablation breakdown under action of a pulsed laser beam so that plasma is produced, an OPO laser adjusts output laser wavelength to resonance excitation wavelength of theatom of an element to be detected, a position adjustment mechanism is adjusted to control movement of an OPO focusing lens three-dimensional space so that the OPO laser beam is focused to the centerof the plasma, the atom of the element to be detected absorbs the energy and produces transition so that a fluorescence spectrum is produced, a spherical mirror carries out spatial constraint on the plasma so that many plasmas as possible are focused by a plasma collection achromatic lens assembly and then collected and transmitted to a spectrometer through a fiber optic probe, the spectrometer converts the received spectral signal into an electrical signal and transmits it to a processing terminal and the processing terminal compares the detected spectrum so that fast, accurate and eco-friendly detection is realized.

The invention relates to the field of veterinary drug residue detection, in particular to an analysis method for efficiently simultaneously detecting thiamphenicol, florfenicol and florfenicol amine multiple residues in poultry eggs, poultry meat and pork. The analysis method includes steps of 1), extracting and purifying poultry egg, poultry meat and pork samples; 2), carrying out ultrahigh-performance liquid chromatography-fluorescence detection. The ultrahigh-performance liquid chromatography-fluorescence detection includes a ultrahigh-performance liquid chromatography conditions and b fluorescence detection. The ultrahigh-performance liquid chromatography conditions include that aqueous solution with 0.005 mol / L of NaH2PO4, 0.003 mol / L of sodium lauryl sulfate and 0.05% of triethylamine is used as mobile phase A, and acetonitrile is used as mobile phase B. The analysis method has the advantages that the residues of the thiamphenicol, the florfenicol and the florfenicol amine in thepoultry eggs (chicken eggs, duck eggs, goose eggs, pigeon eggs and quail eggs), the poultry meat (chicken, duck meat and goose meat) and the pork can be efficiently and accurately simultaneously detected by the aid of the analysis method, and the florfenicol amine is metabolite of the florfenicol; the requirements of the Ministry of Agriculture of China, the FDA (Food and Drug Administration) ofthe America and the European Union (EU) on methods for detecting veterinary drug residues can be met.

The invention discloses a color difference meter assessment method for pork color. The method comprises the following steps of: cutting a pork block of which the thickness is not less than 2 centimeters from longissimus dorsi on a pig thoracolumbar spine joint which is fully cooled at the ambient temperature of 0-4 DEG C along a direction which is vertical to the trend of muscles, putting the cut pork block on a red plastic plate with a newly-cut surface facing upwards, wrapping the pork block with a single-layer colorless transparent fresh-keeping film, attaching the film to the pork block to avoid air bubbles and wrinkles, and developing color at the temperature of 0-4 DEG C for 30-60 minutes; and assessing pork color by using a color difference meter. Key points of the method are that: four factors, namely, pork sample thickness, background color, oxygenation temperature and color developing time, which influence the color difference meter assessment method for pork color are researched, so that a standardized operation specification is provided for the color difference meter assessment method of the pork color.

The invention relates to a method for rapidly detecting beta receptor agonist in pork. The method comprises the steps of: 1) weighing a pork sample, carrying out homogenizing and enzymatic hydrolysis,adding an extracting solution, carrying out vortex oscillation for 10-15 minutes and centrifuging for 5-10 minutes at the speed of 8000rpm to obtain supernatant for later use; 2) After pretreating anMCX (Mixed-mode Cation eXchange) solid phase extraction column, loading the supernatant of the step 1) onto the MCX solid phase extraction column, washing by using deionized water, draining the MCX solid phase under negative pressure, eluting the MCX solid phase extraction column by using eluent, concentrating, drying and diluting by using a 0.1% of formic acid solution to obtain a sample liquid;and 3) loading the sample liquid onto an ultra-high performance liquid chromatograph to detect the content of the beta receptor agonist in the sample liquid. The method disclosed by the invention hasthe advantages that the enzymatic hydrolysis time is significantly shortened by optimizing the enzymatic hydrolysis conditions; the pork sample can be quickly pretreated through extraction and elution; a satisfactory separation effect can be quickly obtained when the sample loading and detection are carried out by using the ultra-high performance liquid chromatograph of the method; and the methodis simple and convenient to operate, fast and high in efficiency and is suitable for the detection of large-batch samples.

The invention discloses a method and a system for rapidly screening and detecting characteristic metabolic volatile matters of meat products, which are characterized in that the characteristic metabolic volatile matters of the meat products are used as research objects, and characteristic information of each metabolic volatile matter is represented by different retention times and ionic strengths of compounds; data visualization is realized by utilizing a MetaboAnalyst data analyzing system and combining metabonomics analyzing methods such as multivariate statistics and the like, so that the characteristic metabolism volatile matters of the pork sample are quickly and accurately screened out. Meanwhile, a portable smart Raman spectrum detection system is developed, integrated, miniaturized and portable Raman spectrum equipment is developed, and a cloud computing model resource library and a Raman spectrum cloud analyzing platform capable of being remotely called are constructed. Raman spectrum data of an independent unknown sample set is collected, and a detection result is obtained by using a cloud computing model. The invention provides a new idea for rapid screening and detection of meat product characteristic metabolism volatile matters, develops a new technical field, and can overcome the limitations of complexity and poor accuracy of the existing detection method.

The invention discloses a visual detecting method for a pork maturity condition. The visual detecting method comprises the following steps: (1) judging a maturity category of a pork sample according to physical and chemical indexes of the pork sample; (2) scanning the pork sample by using a near-infrared hyperspectral meter to obtain a three-dimensional hyperspectral image; (3) extracting spectral images of the pork sample under five characteristic wavelengths; (4) establishing a three-layer neural network; (5) scanning the to-be-detected pork sample by using the near-infrared hyperspectral meter, and obtaining the maturity category result of each pixel of the three-dimensional hyperspectral image of the to-be-detected pork sample by virtue of the three-layer neural network; (6) respectively representing three maturity categories with three colors to obtain a distribution diagram of the pork maturity condition of the to-be-detected pork, and judging the pork maturity condition. The visual detecting method for the pork maturity condition disclosed by the invention can be used for quickly judging the grade of maturity of livestock meat without damages, so that both physical and chemical indexes are not needed to be measured, and a professional training spectral model is also not needed.

The invention discloses a water-saving brushing device for blind roads, which comprises a car body and a water pump; rollers are arranged under the car body; a scrubbing barrel is arranged on the left side of the car body, and the brushing barrel is vertically arranged and the top of the scrubbing barrel is connected to the bottom of the car body. The surface is fixedly connected; the lower end of the brushing cylinder is provided with a scrubbing tank; the brushing cylinder is provided with a brushing roller; the left inner wall of the brushing cylinder is provided with a water spray head; the water spray head is connected with a water outlet pipe, and the water outlet pipe extends To the top of the car body and connected with a water pump. The present invention is equipped with a crushing cylinder and a crushing blade group whose distribution density gradually increases from top to bottom in the crushing cylinder to efficiently crush pork samples, which is convenient for subsequent extrusion of pork; the present invention is provided with a hydraulically driven extrusion disc And the squeeze cylinder, the crushed pork sample is quickly squeezed, and the water content of the pork is measured with a measuring cylinder, which is more efficient than the traditional squeeze of the whole pork sample.

The invention provides a method for detecting the freshness of pork by using FAIMS. The steps include: (1) taking a pork sample to make a pork sample liquid; (2) FAIMS detection: taking a pork sample liquid and adding it to a sampler, controlling the sampler The temperature is 20°C to 90°C to volatilize the pork sample liquid and form an equilibrium gas of trimethylamine, and then pass a carrier gas with a flow rate of 500mL / min to 3500mL / min into the equilibrium gas of trimethylamine to form a mixed gas , input the mixed gas into the FAIMS detector for detection, and control the pressure in the FAIMS detector to be 0.5bar-2.5bar. The method for detecting the freshness of pork by FAIMS described in the present invention obtains the standard for evaluating the freshness of pork by detecting the ion current value of the characteristic peak of trimethylamine in the process of changing the freshness of the pork sample, which has high accuracy and is easy to operate , low detection cost.

The invention discloses a rapid detection method for zilpaterol residues in pork, belonging to the field of food detection. The present invention uses density functional theory and the standard solution of zilpaterol to determine the Raman characteristic peak of zilpaterol; the Raman characteristic peak signal is strong and the characteristic peak of peak shape is 1113cm ‑1 Establish quantitative analysis curve Y=5214.7X‑35.844, correlation coefficient R 2 It is 0.9355; Afterwards, the pork sample is pretreated and extracted with an extraction solvent to obtain a test solution; then, according to the Raman spectrum and the standard curve of the test solution, the concentration of zilpaterol in the test sample is obtained by testing. The minimum detectable concentration of zilpaterol in pork detected by the method of the present invention is 5 mg / L, qualitative and quantitative analysis can be performed on the residue of zilpaterol in pork, and the detection time of a single sample is controlled within 3 minutes.