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30 results about "Zilpaterol" patented technology
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Zilpaterol (zilpaterol hydrochloride; codenamed RU 42173) is a β₂ adrenergic agonist. Under its trade name, Zilmax, it is used to increase the size of cattle and the efficiency of feeding them. Zilmax is produced by Intervet, a subsidiary of Merck & Co., and marketed as a "beef-improvement technology". Zilpaterol is typically fed in the last three to six weeks of cattle's lives, with a brief period (three days in the US) before death for withdrawal, which allows the drug to mostly leave the animal's tissues.
Method of feeding the cattle with feed additives that increases beef production and reduces liver abscess
The present inventions relates to a method of increasing beef production in cattle with feed additives, comprising feeding cattle with feed, comprising an effective amount of an ionophore in combination with a macrolide antibiotic, and thereafter feeding cattle with feed, comprising zilpaterol and essentially no ionophore or macrolide antibiotic for the succeeding about 20 to 40 days.
Owner:INTERVET INT BV
Method of feeding the cattle with feed additives that increases beef production and reduces liver abscess
The present inventions relates to a method of increasing beef production in cattle with feed additives, comprising feeding cattle with feed, comprising an effective amount of an ionophore in combination with a macrolide antibiotic, and thereafter feeding cattle with feed, comprising zilpaterol and essentially no ionophore or macrolide antibiotic for the succeeding about 20 to 40 days.
Owner:INTERVET INT BV
Zilpaterol immune colloidal gold detection card and preparation method thereof
The invention discloses a zilpaterol immune colloidal gold detection card and a preparation method thereof, and relates to the beta-epinephrine receptor agonist detection technical field. A test strip in an outer shell of the detection card is composed of a PVC rubber plate, a sample pad, a colloidal gold combined pad, a coating film and a water-absorbing pad; a colloidal gold film is a glass cellulose film containing a zilpaterol monoclonal antibody, and the coating film is a nitrocellulose film; a T line and a C line are arranged on the nitrocellulose film, the T line is coated with a zilpaterol-protein conjugate, and the C line is coated with a sheep anti-mouse IgG antibody. The detection card is effectively used for rapid detection of zilpaterol, is convenient and fast, and has accurate results.
Owner:JIANGSU WISE SCI & TECH DEV
Kit for detecting zilpaterol by direct competitive ELISA (enzyme-linked immunosorbent assay) method
ActiveCN101571542ALow pre-processing requirementsThe pre-processing process is simpleColor/spectral properties measurementsBiological testingZilpaterolHigh rate
The invention discloses a kit for detecting zilpaterol by the direct competitive ELISA (enzyme-linked immunosorbent assay) method. The kit provided by the invention comprises a zilpaterol specific antibody, an ELISA plate coated with the zilpaterol specific antibody, zilpaterol standard, a zilpaterol HRP (horse radish peroxidase) label and a working solution. The ELISA kit for detecting zipaterolis sensitive, high-rate and accurate, so that the ELISA kit is mainly used for screening large quantities of samples; the major reagent in the kit is provided in the form of working solutions, so thatthe application is convenient, and the kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like; and the kit can rapidly detect the residual zilpaterol in feed and animal products.
Owner:浙江迪恩生物科技股份有限公司
Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-JK][1]-benzazepin-2[1H]-one and zilpaterol
ActiveUS20100173892A1Increased rate of weight gainImprove feeding efficiencyBiocideOrganic chemistryBenzeneZilpaterol
This invention relates to a process for the hydrogenation of a ketooxime to selectively form an aminoalcohol stereoisomer, and, in particular, to a process for the hydrogenation of 4,5-dihydro-imidazo[4,5,1-jk][1]benzazepin-2,6,7[1H]-trione-6-oxime or a salt thereof to selectively form a stereoisomer of 6-amino-7-hydroxy-4,5,6, 7-tetrahydro-imidazo[4,5,1-jk][1]-benzazepin-2[1H]-one or a salt thereof. This invention also relates to the use of the 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-jk][1]-benzazepin-2[1H]-one hydrogenation product or a salt thereof to selectively make a stereoisomer of zilpaterol or a salt thereof, as well as the use of such a zilpaterol stereoisomer or salt in methods of treatment and medicaments for animals.
![Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-JK][1]-benzazepin-2[1H]-one and zilpaterol](https://images-eureka.patsnap.com/patent_img/5f4da205-220c-4b29-8d42-e3c9395edccf/US20100173892A1-20100708-C00001.png "Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-JK][1]-benzazepin-2[1H]-one and zilpaterol")
![Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-JK][1]-benzazepin-2[1H]-one and zilpaterol](https://images-eureka.patsnap.com/patent_img/5f4da205-220c-4b29-8d42-e3c9395edccf/US20100173892A1-20100708-C00002.png "Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-JK][1]-benzazepin-2[1H]-one and zilpaterol")
![Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-JK][1]-benzazepin-2[1H]-one and zilpaterol](https://images-eureka.patsnap.com/patent_img/5f4da205-220c-4b29-8d42-e3c9395edccf/US20100173892A1-20100708-C00003.png "Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-JK][1]-benzazepin-2[1H]-one and zilpaterol")
Owner:INTERVET INT BV
Method and test strip for detecting zilpaterol, and application of test strip
The invention discloses a method and test strip for detecting zilpaterol, and application of the test strip. The method is a combination of colloidal-gold immunochromatography assay and competitive immunochromatography assay; an antibody used in the method is a colloidal-gold-labeled monoclonal antibody against zilpaterol, and competitive antigen zilpaterol is immobilized on a detection line of the test strip; a to-be-tested sample reacts with the colloidal-gold-labeled monoclonal antibody against zilpaterol at first and then with the competitive antigen; when a red strip does not appear on the detection line, it is determined that the to-be-tested sample contains zilpaterol; and when a red strip appears on the detection line, it is determined that the to-be-tested sample does not contain zilpaterol or has low zilpaterol content which does not exceed detection limit. The test strip comprises a base plate, and a sample absorption pad, a bonding pad, a chromatographic membrane and a water absorbing pad which are all adhered on the base plate and closely connected in sequence, wherein the bonding pad is provided with the colloidal-gold antibody, and the chromatographic membrane is provided with a detection line, a quality control line and the detection line. When the test strip is used to detect a positive sample, no color is displayed on the detection line.
Owner:JINAN UNIVERSITY
Enzyme-linked immunosorbent assay kit for detecting residual zilpaterol and use method thereof
InactiveCN103808921APreparing sample for investigationColor/spectral properties measurementsAntigenSerum ige
The invention discloses an enzyme-linked immunosorbent assay kit for detecting residual zilpaterol and a use method thereof, and belongs to the technical field of enzyme-linked immunosorbent assay (ELISA). The kit includes an ELISA plate coated with a zilpaterol antigen, a zilpaterol standard substance working solution, a zilpaterol antibody working solution, a zilpaterol enzyme-labeled second antibody working solution, a substrate solution, a stopping liquid, a concentrated washing liquid and a concentrated sample diluent. The kit adopts an indirect competitive ELISA, and residual zilpaterol in a standard substance or a to-be-detected sample and a pre-coated antigen on the ELISA plate jointly compete for the zilpaterol antibody. The method can be used for direct detection of zilpaterol in animal derived foods, urine samples, animal tissues and serum samples, has the characteristics of convenience, rapidness, sensitivity and the like, and is suitable for detection of samples on a large scale; and the sensitivity degree of the kit is 0.1 ng / mL.
Owner:JIANGSU WISE SCI & TECH DEV
Chemiluminiscence immunoassay kit for detecting zilpaterol
InactiveCN103808933AReduce testing costsThe electrophoretic method is fast and easyChemiluminescene/bioluminescenceBiological testingCarrier proteinChemiluminescence
The invention provides a chemiluminiscence immunoassay kit for detecting zilpaterol, and belongs to the field of immunological detection. The kit comprises a non-transparent white ELISA plate coated with a zilpaterol-carrier protein conjugate, a zilpaterol standard substance, a zilpaterol-peroxidase labeled antibody working solution, a luminescent substrate solution, a concentrated sample diluent and a concentrated washing liquid. The zilpaterol-carrier protein conjugate is obtained by coupling zilpaterol and a carrier protein through a mixed anhydride method or a carbodiimide method, and the concentrated washing liquid contains 0.05% of Twain-20. Compared with a traditional enzyme-linked immunosorbent assay method, the kit has higher sensitivity degree, is short in detection time and low in detection cost, and can be used for detection of the residual amount of zilpaterol in animal urine, blood samples, tissues, viscera and other samples.
Owner:JIANGSU WISE SCI & TECH DEV
A Method of Improving the Efficiency of Beef Production from Bovine Animals
InactiveUS20160022694A1Improve efficiencyAverage daily gain (ADG) of the animal is increasedBiocideAnimal feeding stuffZilpaterol
A method of improving the efficiency of beef production from a bovine animal which is fed multiple times per day, comprising delivering to the animal an effective dose of zilpaterol in one or more but not all of daily feedings and wherein the zilpaterol is thoroughly mixed into the feed before use.
Owner:INTERVET INC
Method for measuring residual quantities of growth promoting agents such as zilpaterol, cimbuterol, clenproperol and bambuterol in beef
InactiveCN105092762AComponent separationPreparing sample for investigationEthyl acetateGlucuronidase
The invention discloses a method for measuring residual quantities of growth promoting agents such as zilpaterol, cimbuterol, clenproperol and bambuterol in beef. The method includes hydrolyzing beef samples by the aid of beta-glucuronidase / aryl sulfatase; centrifuging the beef samples under the condition of pH (potential of hydrogen) of 1.0+ / -0.2; regulating pH of supernatant until the pH reaches 9.5+ / -0.2; sequentially extracting the supernatant by the aid of ethyl acetate and tert-butyl methyl ether; combining organic phases with one another; drying the organic phases by means of blowing; dissolving the organic phases by the aid of formic acid; enabling the organic phases to pass cation exchange solid-phase extraction columns; sequentially leaching the organic phases by the aid of 2% formic acid, water and methanol; eluting the organic phases by the aid of ammonium methanol; drying eluent by means of blowing; dissolving the organic phases by the aid of formic acid and methanol aqueous solution; filtering the organic phases by the aid of films; feeding filter liquid in liquid chromatograph / mass spectrometers to obtain the residual quantities of the growth promoting agents in the beef according to standard curves. The method has the advantages that the four growth promoting agents including the zilpaterol, the cimbuterol, the clenproperol and the bambuterol can be quickly detected by the aid of the method, and residues of the four growth promoting agents which are four types of medicine in the beef can be monitored.
Owner:LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
Method for utilizing CdTe quantum dots to measure Zzilpaterol
ActiveCN108152257ASimple detection operationReduce testing costsFluorescence/phosphorescenceAntigenZilpaterol
The invention discloses a method for utilizing CdTe quantum dots to measure zZilpaterol. The method comprises the steps that a CdTe-zZilpaterol antigen is prepared; an enzyme-labeledELISA plate coatedwith a zZilpaterol antibody is prepared; a Zzilpaterol standard solution and a CdTe-zZilpaterol antigen solution in the series concentrations are added into holes of the enzyme-labeledELISA plate, meanwhile, a blank control group is set, and incubation and washing are conducted; the fluorescence intensity F of each hole in the enzyme-labeledELISA plate and the fluorescence intensity fF0 of the blank control group are measured, the inhibition ratio I is calculated according to the formula that I=(F-F0) / (Fmax-F0), the inhibition ratio serves is treated as the vertical coordinate, the logarithmof the concentration of the zZilpaterol standard solution serves as the horizontal coordinateabscissa, and a standard curve is drawn; and a to-be-detected sample and the CdTe-zZilpaterol antigen solution are added into the enzyme-labeledELISA plate to be subjected to incubation and washing, the fluorescence intensity is measured, the standard curve is substituted in, and the concentration of zZilpaterol in the to-be-detected sample is obtained. Zilpaterol is measured by utilizing the CdTe quantum dots, the detection cost is low, the detection time is short, the sensitivity is high, and the specificity is good.
Owner:INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
Method for measuring zilpaterol by using CdTe quantum dot
ActiveCN108037105ASimple detection operationReduce testing costsFluorescence/phosphorescenceAntigenZilpaterol
The invention discloses a method for measuring zilpaterol by using a CdTe quantum dot. The method comprises the following steps: preparing a CdTe-zilpaterol antibody; preparing a zilpaterol antigen-coated ELISA plate; adding zilpaterol standard solutions and CdTe-zilpaterol antibody solutions with a series of concentration into each hole of the ELISA plate, setting a blank control group simultaneously, incubating and washing; measuring the fluorescence intensity F of each hole in the ELISA plate, setting the fluorescence intensity of the blank control group as F0, calculating the inhibition rate I which is equal to (F-F0) / (Fmax-F0), and drawing a standard curve by taking the inhibition rate as vertical coordinate and the logarithm of the zilpaterol standard solution concentration as horizontal coordinate; and adding a to-be-detected sample and the CdTe-zilpaterol antibody solution into the ELISA plate, incubating, washing, measuring the fluorescence intensity and substituting into thestandard curve to obtain the zilpaterol concentration of the to-be-detected sample. According to the method, the zilpaterol is measured by the CdTe quantum dot, the detection cost is low, the detection time is short, the sensitivity is high and the specificity is high.
Owner:INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
Zilpaterol compound veterinary composition as well as preparation method and application thereof
ActiveCN108272880ACompatibility is reasonableDefinite curative effectAntibacterial agentsAntiviralsAdditive ingredientVeterinary Drugs
The invention provides a zilpaterol compound veterinary composition. The zilpaterol compound veterinary composition comprises traditional Chinese medicine components and Western medicine components, wherein the traditional Chinese medicine components comprise the following raw material medicines in parts by weight: 15 to 25 parts of astragalus membranaceus, 15 to 25 parts of ephedra, 10 to 20 parts of coptis chinensis, 10 to 20 parts of pyrrosia lingua, 5 to 15 parts of semen armeniacae amarae, 5 to 15 parts of cocklebur fruit and 5 to 10 parts of mint; and the Western medicine components comprise the following raw material medicines in parts by weight: 5 to 7 parts of ribavirin, 3 to 4.5 parts of a zilpaterol and 1 to 3 parts of florfenicol. The invention also provides a preparation method of the veterinary composition as well as application of the veterinary composition to prevention and treatment of canine distemper and poultry leptospirosis. The composition provided by the invention is a traditional Chinese medicine and Western medicine compound preparation and is prepared by selecting and compounding various traditional Chinese herbal medicines and Western medicines, various medicines are reasonable in compatibility, strong synergistic effect is generated after the medicines are composed, and the curative effect can be doubled. The zilpaterol compound veterinary composition can treat both symptoms and root causes, has obvious curative effect and various dosage forms, can perform mixed feeding, mixed drinking and injection, is convenient to use, and has durable medicineeffect and high bioavailability.
Owner:JIANGSU LINGYUN PHARMA
Method for simultaneously detecting 48 stimulants in feed
ActiveCN113588827AGuarantee testing needsHigh sensitivityComponent separationStimulantNandrolone phenylpropionate
The invention relates to a method for simultaneously detecting 48 stimulants in a feed, which comprises the following steps: extracting a sample twice by using methanol, blow-drying the merged supernatant, dissolving the obtained residue by using an acetonitrile aqueous solution, carrying out vortex oscillation and filtering to obtain a to-be-detected solution, determining the stimulant in the to-be-detected solution and the mixed standard working solution by using a high performance liquid chromatography tandem mass spectrometer, comparing the obtained spectrogram information, and judging that a corresponding target object exists in the sample; according to the peak areas of the stimulant to be detected and the corresponding internal standard substance, combining the extraction volume, the sample amount and the dilution ratio of each liquid to be detected in the acquisition process, and determining the contents of zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an external standard method for quantification; determining the content of all stimulants except for zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an internal standard method for quantification.
Owner:西安市食品药品检验所西安市药品不良反应监测中心
One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol
The present invention relates to one pot process for the preparation of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid of Formula-I, which is a key intermediate and its use in the preparation of Zilpaterol, which comprises condensation of methyl-4-chloro butyrate with 1-(prop-1-en-2-yl)-1H-benzo[d]imidazol-2(3H)-one in presence of a base and suitable solvent to give corresponding ester derivative, further hydrolyzation and acidification in presence of inorganic solvent to obtain Formula-I.
![One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol](https://images-eureka.patsnap.com/patent_img/229d3f3a-4ac8-42ac-b4f4-fd64e6ab0541/US20210238146A1-C00001.png "One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol")
![One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol](https://images-eureka.patsnap.com/patent_img/229d3f3a-4ac8-42ac-b4f4-fd64e6ab0541/US20210238146A1-C00002.png "One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol")
![One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol](https://images-eureka.patsnap.com/patent_img/229d3f3a-4ac8-42ac-b4f4-fd64e6ab0541/US20210238146A1-C00003.png "One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol")
Owner:RA CHEM PHARMA
One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol
The present invention relates to one pot process for the preparation of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid of Formula-I, which is a key intermediate and its use in the preparation of Zilpaterol, which comprises condensation of methyl-4-chloro butyrate with 1-(prop-1-en-2-yl)-1H-benzo[d]imidazol-2(3H)-one in presence of a base and suitable solvent to give corresponding ester derivative, further hydrolyzation and acidification in presence of inorganic solvent to obtain Formula-I.
![One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol](https://images-eureka.patsnap.com/patent_img/30c9262e-2481-46bd-89dc-542fa80f58f0/US11214551-C00001.png "One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol")
![One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol](https://images-eureka.patsnap.com/patent_img/30c9262e-2481-46bd-89dc-542fa80f58f0/US11214551-C00002.png "One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol")
![One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol](https://images-eureka.patsnap.com/patent_img/30c9262e-2481-46bd-89dc-542fa80f58f0/US11214551-C00003.png "One pot synthesis of 4-(1,2-dihydro-2-oxobenzo[d]imidazol-3-yl)butanoic acid, a key intermediate of zilpaterol")
Owner:RA CHEM PHARMA
Method for simultaneously detecting 48 stimulants in animal body fluid
ActiveCN113588826AGuarantee testing needsShort analysis timeComponent separationStimulantNandrolone phenylpropionate
The invention relates to a method for simultaneously detecting 48 stimulants in animal body fluid, which comprises the following steps: extracting a sample by using an acetonitrile solution of formic acid, removing water by using anhydrous sodium sulfate, blow-drying the obtained supernatant, dissolving residues by using an aqueous solution of acetonitrile, carrying out vortex oscillation filtration to form a solution to be detected, determining the stimulant in the to-be-detected solution and the mixed standard working solution by using a high performance liquid chromatography tandem mass spectrometer, comparing the obtained spectrogram information, and judging that a corresponding target object exists in the sample; according to the peak areas of the stimulant and the corresponding internal standard substance, combining the extraction volume, the sample amount and the dilution ratio of each to-be-detected solution in the acquisition process, and determining the contents of zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an external standard method for quantification, anddetermining the content of all stimulants except for zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an internal standard method for quantification.
Owner:西安市食品药品检验所西安市药品不良反应监测中心
A zilpaterol monoclonal antibody hybridoma cell line and its application
ActiveCN111909903BImprove featuresHigh detection sensitivitySerum albuminTissue cultureBALB/cAdjuvant
A zilpaterol monoclonal antibody hybridoma cell line and application thereof belong to the technical field of food safety immunoassay. Zilpaterol monoclonal antibody hybridoma cell line SMB1C2 has been deposited in the General Microbiology Center CGMCC of the Chinese Microbiological Culture Collection Management Committee, classified and named as a monoclonal cell strain, and the date of preservation is November 28, 2019, and the preservation number is CGMCC No. 19170. The complete antigen of zilpaterol was mixed and emulsified with the same amount of Freund's adjuvant, and BALB / c mice were immunized by multi-point subcutaneous injection on the back of the neck, and finally a monoclonal antibody hybridoma cell line was obtained. The monoclonal antibody secreted by this cell line has good specificity and detection sensitivity to zilpaterol (IC 50 The value is 0.5ng / mL), which can realize the detection of zilpaterol residues in milk, muscle tissue and urine, and provides raw materials for the immunological detection of zilpaterol residues in food, which has practical application value.
Owner:JIANGNAN UNIV
A method for determining zilpaterol using cdte quantum dots
ActiveCN108152257BSimple detection operationReduce testing costsFluorescence/phosphorescenceAntigenZilpaterol
Owner:INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
Kit for rapidly detecting content of zilpaterol in food and detection method
The invention discloses a kit for rapidly detecting the content of zilpaterol in food and a detection method, and belongs to the technical field of detection, the kit comprises a detection card, an ID chip card and a specification, the detection card is composed of a test strip and a plastic clamping piece, and the test strip comprises 0.5 mg / mL of DNP-BSA and 1 mg / mL of zilpaterol antigen. A quantum dot fluorescence labeling technology and a competitive immunochromatography principle are adopted, a zilpaterol antigen is coated on a certain zone on a nitrocellulose membrane, after a sample is added into a sample adding hole, the sample moves forwards along the membrane due to the capillary action, and when the sample moves to a zone fixed with an antibody, the nitrocellulose membrane is fixed with the zilpaterol antigen. According to the present invention, the corresponding antigen in the sample competes with the antigen, and the quantum dot can make the region not display the fluorescence signal so as to achieve the specific immunodiagnosis, the detection needs to be equipped with the corresponding instrument, the instrument price is low, the size is small, the operation is simple and convenient, the bedside detection can be achieved, the time consumption is about 3-10 min, the sensitivity is high, and the sample consumption is low.
Owner:深圳容金科技有限公司
Kit for detecting zilpaterol by direct competitive ELISA (enzyme-linked immunosorbent assay) method
ActiveCN101571542BLow pre-processing requirementsThe pre-processing process is simpleColor/spectral properties measurementsBiological testingZilpaterolElisa kit
The invention discloses a kit for detecting zilpaterol by the direct competitive ELISA (enzyme-linked immunosorbent assay) method. The kit provided by the invention comprises a zilpaterol specific antibody, an ELISA plate coated with the zilpaterol specific antibody, zilpaterol standard, a zilpaterol HRP (horse radish peroxidase) label and a working solution. The ELISA kit for detecting zipaterolis sensitive, high-rate and accurate, so that the ELISA kit is mainly used for screening large quantities of samples; the major reagent in the kit is provided in the form of working solutions, so thatthe application is convenient, and the kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like; and the kit can rapidly detect the residual zilpaterol in feed and animal products.
Owner:浙江迪恩生物科技股份有限公司
A method for determination of growth promoter residues of zilpaterol, sibuterol, clenpro and bambuterol in beef
The invention discloses a method for measuring residual quantities of growth promoting agents such as zilpaterol, cimbuterol, clenproperol and bambuterol in beef. The method includes hydrolyzing beef samples by the aid of beta-glucuronidase / aryl sulfatase; centrifuging the beef samples under the condition of pH (potential of hydrogen) of 1.0+ / -0.2; regulating pH of supernatant until the pH reaches 9.5+ / -0.2; sequentially extracting the supernatant by the aid of ethyl acetate and tert-butyl methyl ether; combining organic phases with one another; drying the organic phases by means of blowing; dissolving the organic phases by the aid of formic acid; enabling the organic phases to pass cation exchange solid-phase extraction columns; sequentially leaching the organic phases by the aid of 2% formic acid, water and methanol; eluting the organic phases by the aid of ammonium methanol; drying eluent by means of blowing; dissolving the organic phases by the aid of formic acid and methanol aqueous solution; filtering the organic phases by the aid of films; feeding filter liquid in liquid chromatograph / mass spectrometers to obtain the residual quantities of the growth promoting agents in the beef according to standard curves. The method has the advantages that the four growth promoting agents including the zilpaterol, the cimbuterol, the clenproperol and the bambuterol can be quickly detected by the aid of the method, and residues of the four growth promoting agents which are four types of medicine in the beef can be monitored.
Owner:LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-jk][1]-benzazepin-2[1H]-one and zilpaterol
ActiveUS8629134B2Increased rate of weight gainImprove feeding efficiencyBiocideOrganic chemistryZilpaterolEnantioselective synthesis
This invention relates to a process for the hydrogenation of a ketooxime to selectively form an aminoalcohol stereoisomer, and, in particular, to a process for the hydrogenation of 4,5-dihydro-imidazo[4,5,1-jk][1]benzazepin-2,6,7[1H]-trione-6-oxime or a salt thereof to selectively form a stereoisomer of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-jk][1]-benzazepin-2[1H]-one or a salt thereof. This invention also relates to the use of the 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-jk][1]-benzazepin-2[1H]-one hydrogenation product or a salt thereof to selectively make a stereoisomer of zilpaterol or a salt thereof, as well as the use of such a zilpaterol stereoisomer or salt in methods of treatment and medicaments for animals.
![Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-jk][1]-benzazepin-2[1H]-one and zilpaterol](https://images-eureka.patsnap.com/patent_img/ae2ef5ea-84b3-4d24-a143-9a5da01ef593/US08629134-20140114-C00001.png "Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-jk][1]-benzazepin-2[1H]-one and zilpaterol")
![Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-jk][1]-benzazepin-2[1H]-one and zilpaterol](https://images-eureka.patsnap.com/patent_img/ae2ef5ea-84b3-4d24-a143-9a5da01ef593/US08629134-20140114-C00002.png "Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-jk][1]-benzazepin-2[1H]-one and zilpaterol")
![Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-jk][1]-benzazepin-2[1H]-one and zilpaterol](https://images-eureka.patsnap.com/patent_img/ae2ef5ea-84b3-4d24-a143-9a5da01ef593/US08629134-20140114-C00003.png "Enantioselective synthesis of 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-jk][1]-benzazepin-2[1H]-one and zilpaterol")
Owner:INTERVET INT BV
Method for simultaneously detecting 48 stimulants in animal-derived food
ActiveCN113588828AAvoid decompositionGuarantee testing needsComponent separationBiotechnologyNandrolone phenylpropionate
The invention relates to a method for simultaneously detecting 48 stimulants in animal-derived food, which comprises the following steps of: performing extracting and dewatering according to different types of samples, blow-drying the obtained supernate at a proper temperature, performing vortex oscillation and filtration on the residues obtained by dissolving the reagent to form respective to-be-detected solutions, determining the stimulant in the to-be-detected solution and the mixed standard working solution by using a high performance liquid chromatography tandem mass spectrometer, comparing the obtained spectrogram information, and judging that a corresponding target object exists in the sample; according to the peak areas of the stimulant and the corresponding internal standard substance, combining the extraction volume, the sample amount and the dilution ratio of each to-be-detected solution in the acquisition process, and determining the contents of zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an external standard method for quantification, and determining the content of all stimulants except for zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an internal standard method for quantification.
Owner:西安市食品药品检验所西安市药品不良反应监测中心
Pre-mix composition for cattle
ActiveUS9364483B2Increased rate of weight gainImprove meat qualityPowder deliveryFood processingBiotechnologyZilpaterol
The present invention concerns a premix composition for feeding cattle, comprising:a) a premix carrier having an overall particle size comprised between 300 and 400 μm,b) zilpaterol, andc) a surface agent.The present invention is also related to a method of increasing the rate of weight gain in cattle, comprising the administration to the cattle of feed additives consisting of a premix composition such as described, over a period of at least two weeks.
Owner:LAB VIRBAC A JOINT CO
A kind of synthetic method of zilpaterol hydrochloride
Owner:JIANGSU LINGYUN PHARMA
A method of enhancing performance in broiler chickens
A method of enhancing the performance of chicken comprising administering zilpaterol to the chicken wherein the concentration of zilpaterol is from about 1 ppm to about 13 ppm and is administered every day for a period of about 7 to about 21 days.
Owner:INTERVET INT BV
A method for determining zilpaterol using cdte quantum dots
ActiveCN108037105BSimple detection operationReduce testing costsFluorescence/phosphorescenceAntigenZilpaterol
The invention discloses a method for measuring zilpaterol by using a CdTe quantum dot. The method comprises the following steps: preparing a CdTe-zilpaterol antibody; preparing a zilpaterol antigen-coated ELISA plate; adding zilpaterol standard solutions and CdTe-zilpaterol antibody solutions with a series of concentration into each hole of the ELISA plate, setting a blank control group simultaneously, incubating and washing; measuring the fluorescence intensity F of each hole in the ELISA plate, setting the fluorescence intensity of the blank control group as F0, calculating the inhibition rate I which is equal to (F-F0) / (Fmax-F0), and drawing a standard curve by taking the inhibition rate as vertical coordinate and the logarithm of the zilpaterol standard solution concentration as horizontal coordinate; and adding a to-be-detected sample and the CdTe-zilpaterol antibody solution into the ELISA plate, incubating, washing, measuring the fluorescence intensity and substituting into thestandard curve to obtain the zilpaterol concentration of the to-be-detected sample. According to the method, the zilpaterol is measured by the CdTe quantum dot, the detection cost is low, the detection time is short, the sensitivity is high and the specificity is high.
Owner:INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
A kind of zilpaterol compound veterinary drug composition and its preparation method and application
ActiveCN108272880BCompatibility is reasonableDefinite curative effectAntibacterial agentsAntiviralsVeterinary DrugsPharmaceutical Substances
The invention provides a zilpaterol compound veterinary drug composition, which comprises Chinese medicine ingredients and western medicine ingredients. The Chinese medicine ingredients include the following raw materials in parts by weight: 15-25 parts of Astragalus, 15-25 parts of Ephedra, and 10-25 parts of Coptidis 20 parts, Shi Wei 10-20 parts, bitter almond 5-15 parts, cocklebur 5-15 parts, peppermint 5-10 parts; Western medicine ingredients include the following raw materials: ribavirin 5-7 parts, zippat Luo 3-4.5 parts, Florfenicol 1-3 parts. The invention also provides a preparation method of the veterinary drug composition and its application in preventing and treating canine distemper and poultry leptospirosis. The composition provided by the invention is a compound preparation of Chinese and western medicines, which is compounded by selecting multi-flavored Chinese herbal medicines and western medicines. The compatibility of the various medicines is reasonable, and each medicine produces a strong synergistic effect after being composed, and can double the curative effect. It treats both symptoms and root causes, has definite curative effect, and has various dosage forms, which can be mixed feeding, mixed drinking, and injection. It is convenient to use, durable in efficacy, and high in bioavailability.
Owner:JIANGSU LINGYUN PHARMA
Pre-mix composition for cattle
ActiveUS20150366875A1Increased rate of weight gainImprove meat qualityPowder deliveryBiocideBiotechnologyZilpaterol
The present invention concerns a premix composition for feeding cattle, comprising:a) a premix carrier having an overall particle size comprised between 300 and 400 μm,b) zilpaterol, andc) a surface agent.The present invention is also related to a method of increasing the rate of weight gain in cattle, comprising the administration to the cattle of feed additives consisting of a premix composition such as described, over a period of at least two weeks.
Owner:LAB VIRBAC A JOINT CO
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