30 results about "Zilpaterol" patented technology

The present inventions relates to a method of increasing beef production in cattle with feed additives, comprising feeding cattle with feed, comprising an effective amount of an ionophore in combination with a macrolide antibiotic, and thereafter feeding cattle with feed, comprising zilpaterol and essentially no ionophore or macrolide antibiotic for the succeeding about 20 to 40 days.

The invention discloses a zilpaterol immune colloidal gold detection card and a preparation method thereof, and relates to the beta-epinephrine receptor agonist detection technical field. A test strip in an outer shell of the detection card is composed of a PVC rubber plate, a sample pad, a colloidal gold combined pad, a coating film and a water-absorbing pad; a colloidal gold film is a glass cellulose film containing a zilpaterol monoclonal antibody, and the coating film is a nitrocellulose film; a T line and a C line are arranged on the nitrocellulose film, the T line is coated with a zilpaterol-protein conjugate, and the C line is coated with a sheep anti-mouse IgG antibody. The detection card is effectively used for rapid detection of zilpaterol, is convenient and fast, and has accurate results.

The invention discloses a kit for detecting zilpaterol by the direct competitive ELISA (enzyme-linked immunosorbent assay) method. The kit provided by the invention comprises a zilpaterol specific antibody, an ELISA plate coated with the zilpaterol specific antibody, zilpaterol standard, a zilpaterol HRP (horse radish peroxidase) label and a working solution. The ELISA kit for detecting zipaterolis sensitive, high-rate and accurate, so that the ELISA kit is mainly used for screening large quantities of samples; the major reagent in the kit is provided in the form of working solutions, so thatthe application is convenient, and the kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like; and the kit can rapidly detect the residual zilpaterol in feed and animal products.

This invention relates to a process for the hydrogenation of a ketooxime to selectively form an aminoalcohol stereoisomer, and, in particular, to a process for the hydrogenation of 4,5-dihydro-imidazo[4,5,1-jk][1]benzazepin-2,6,7[1H]-trione-6-oxime or a salt thereof to selectively form a stereoisomer of 6-amino-7-hydroxy-4,5,6, 7-tetrahydro-imidazo[4,5,1-jk][1]-benzazepin-2[1H]-one or a salt thereof. This invention also relates to the use of the 6-amino-7-hydroxy-4,5,6,7-tetrahydro-imidazo[4,5,1-jk][1]-benzazepin-2[1H]-one hydrogenation product or a salt thereof to selectively make a stereoisomer of zilpaterol or a salt thereof, as well as the use of such a zilpaterol stereoisomer or salt in methods of treatment and medicaments for animals.

The invention discloses a method and test strip for detecting zilpaterol, and application of the test strip. The method is a combination of colloidal-gold immunochromatography assay and competitive immunochromatography assay; an antibody used in the method is a colloidal-gold-labeled monoclonal antibody against zilpaterol, and competitive antigen zilpaterol is immobilized on a detection line of the test strip; a to-be-tested sample reacts with the colloidal-gold-labeled monoclonal antibody against zilpaterol at first and then with the competitive antigen; when a red strip does not appear on the detection line, it is determined that the to-be-tested sample contains zilpaterol; and when a red strip appears on the detection line, it is determined that the to-be-tested sample does not contain zilpaterol or has low zilpaterol content which does not exceed detection limit. The test strip comprises a base plate, and a sample absorption pad, a bonding pad, a chromatographic membrane and a water absorbing pad which are all adhered on the base plate and closely connected in sequence, wherein the bonding pad is provided with the colloidal-gold antibody, and the chromatographic membrane is provided with a detection line, a quality control line and the detection line. When the test strip is used to detect a positive sample, no color is displayed on the detection line.

The invention discloses an enzyme-linked immunosorbent assay kit for detecting residual zilpaterol and a use method thereof, and belongs to the technical field of enzyme-linked immunosorbent assay (ELISA). The kit includes an ELISA plate coated with a zilpaterol antigen, a zilpaterol standard substance working solution, a zilpaterol antibody working solution, a zilpaterol enzyme-labeled second antibody working solution, a substrate solution, a stopping liquid, a concentrated washing liquid and a concentrated sample diluent. The kit adopts an indirect competitive ELISA, and residual zilpaterol in a standard substance or a to-be-detected sample and a pre-coated antigen on the ELISA plate jointly compete for the zilpaterol antibody. The method can be used for direct detection of zilpaterol in animal derived foods, urine samples, animal tissues and serum samples, has the characteristics of convenience, rapidness, sensitivity and the like, and is suitable for detection of samples on a large scale; and the sensitivity degree of the kit is 0.1 ng/mL.

The invention discloses a method for measuring residual quantities of growth promoting agents such as zilpaterol, cimbuterol, clenproperol and bambuterol in beef. The method includes hydrolyzing beef samples by the aid of beta-glucuronidase/aryl sulfatase; centrifuging the beef samples under the condition of pH (potential of hydrogen) of 1.0+/-0.2; regulating pH of supernatant until the pH reaches 9.5+/-0.2; sequentially extracting the supernatant by the aid of ethyl acetate and tert-butyl methyl ether; combining organic phases with one another; drying the organic phases by means of blowing; dissolving the organic phases by the aid of formic acid; enabling the organic phases to pass cation exchange solid-phase extraction columns; sequentially leaching the organic phases by the aid of 2% formic acid, water and methanol; eluting the organic phases by the aid of ammonium methanol; drying eluent by means of blowing; dissolving the organic phases by the aid of formic acid and methanol aqueous solution; filtering the organic phases by the aid of films; feeding filter liquid in liquid chromatograph/mass spectrometers to obtain the residual quantities of the growth promoting agents in the beef according to standard curves. The method has the advantages that the four growth promoting agents including the zilpaterol, the cimbuterol, the clenproperol and the bambuterol can be quickly detected by the aid of the method, and residues of the four growth promoting agents which are four types of medicine in the beef can be monitored.

The invention discloses a method for utilizing CdTe quantum dots to measure zZilpaterol. The method comprises the steps that a CdTe-zZilpaterol antigen is prepared; an enzyme-labeledELISA plate coatedwith a zZilpaterol antibody is prepared; a Zzilpaterol standard solution and a CdTe-zZilpaterol antigen solution in the series concentrations are added into holes of the enzyme-labeledELISA plate, meanwhile, a blank control group is set, and incubation and washing are conducted; the fluorescence intensity F of each hole in the enzyme-labeledELISA plate and the fluorescence intensity fF0 of the blank control group are measured, the inhibition ratio I is calculated according to the formula that I=(F-F0)/(Fmax-F0), the inhibition ratio serves is treated as the vertical coordinate, the logarithmof the concentration of the zZilpaterol standard solution serves as the horizontal coordinateabscissa, and a standard curve is drawn; and a to-be-detected sample and the CdTe-zZilpaterol antigen solution are added into the enzyme-labeledELISA plate to be subjected to incubation and washing, the fluorescence intensity is measured, the standard curve is substituted in, and the concentration of zZilpaterol in the to-be-detected sample is obtained. Zilpaterol is measured by utilizing the CdTe quantum dots, the detection cost is low, the detection time is short, the sensitivity is high, and the specificity is good.

The invention discloses a method for measuring zilpaterol by using a CdTe quantum dot. The method comprises the following steps: preparing a CdTe-zilpaterol antibody; preparing a zilpaterol antigen-coated ELISA plate; adding zilpaterol standard solutions and CdTe-zilpaterol antibody solutions with a series of concentration into each hole of the ELISA plate, setting a blank control group simultaneously, incubating and washing; measuring the fluorescence intensity F of each hole in the ELISA plate, setting the fluorescence intensity of the blank control group as F0, calculating the inhibition rate I which is equal to (F-F0)/(Fmax-F0), and drawing a standard curve by taking the inhibition rate as vertical coordinate and the logarithm of the zilpaterol standard solution concentration as horizontal coordinate; and adding a to-be-detected sample and the CdTe-zilpaterol antibody solution into the ELISA plate, incubating, washing, measuring the fluorescence intensity and substituting into thestandard curve to obtain the zilpaterol concentration of the to-be-detected sample. According to the method, the zilpaterol is measured by the CdTe quantum dot, the detection cost is low, the detection time is short, the sensitivity is high and the specificity is high.

The invention provides a zilpaterol compound veterinary composition. The zilpaterol compound veterinary composition comprises traditional Chinese medicine components and Western medicine components, wherein the traditional Chinese medicine components comprise the following raw material medicines in parts by weight: 15 to 25 parts of astragalus membranaceus, 15 to 25 parts of ephedra, 10 to 20 parts of coptis chinensis, 10 to 20 parts of pyrrosia lingua, 5 to 15 parts of semen armeniacae amarae, 5 to 15 parts of cocklebur fruit and 5 to 10 parts of mint; and the Western medicine components comprise the following raw material medicines in parts by weight: 5 to 7 parts of ribavirin, 3 to 4.5 parts of a zilpaterol and 1 to 3 parts of florfenicol. The invention also provides a preparation method of the veterinary composition as well as application of the veterinary composition to prevention and treatment of canine distemper and poultry leptospirosis. The composition provided by the invention is a traditional Chinese medicine and Western medicine compound preparation and is prepared by selecting and compounding various traditional Chinese herbal medicines and Western medicines, various medicines are reasonable in compatibility, strong synergistic effect is generated after the medicines are composed, and the curative effect can be doubled. The zilpaterol compound veterinary composition can treat both symptoms and root causes, has obvious curative effect and various dosage forms, can perform mixed feeding, mixed drinking and injection, is convenient to use, and has durable medicineeffect and high bioavailability.

The invention relates to a method for simultaneously detecting 48 stimulants in animal body fluid, which comprises the following steps: extracting a sample by using an acetonitrile solution of formic acid, removing water by using anhydrous sodium sulfate, blow-drying the obtained supernatant, dissolving residues by using an aqueous solution of acetonitrile, carrying out vortex oscillation filtration to form a solution to be detected, determining the stimulant in the to-be-detected solution and the mixed standard working solution by using a high performance liquid chromatography tandem mass spectrometer, comparing the obtained spectrogram information, and judging that a corresponding target object exists in the sample; according to the peak areas of the stimulant and the corresponding internal standard substance, combining the extraction volume, the sample amount and the dilution ratio of each to-be-detected solution in the acquisition process, and determining the contents of zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an external standard method for quantification, anddetermining the content of all stimulants except for zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an internal standard method for quantification.

The invention discloses a kit for rapidly detecting the content of zilpaterol in food and a detection method, and belongs to the technical field of detection, the kit comprises a detection card, an ID chip card and a specification, the detection card is composed of a test strip and a plastic clamping piece, and the test strip comprises 0.5 mg/mL of DNP-BSA and 1 mg/mL of zilpaterol antigen. A quantum dot fluorescence labeling technology and a competitive immunochromatography principle are adopted, a zilpaterol antigen is coated on a certain zone on a nitrocellulose membrane, after a sample is added into a sample adding hole, the sample moves forwards along the membrane due to the capillary action, and when the sample moves to a zone fixed with an antibody, the nitrocellulose membrane is fixed with the zilpaterol antigen. According to the present invention, the corresponding antigen in the sample competes with the antigen, and the quantum dot can make the region not display the fluorescence signal so as to achieve the specific immunodiagnosis, the detection needs to be equipped with the corresponding instrument, the instrument price is low, the size is small, the operation is simple and convenient, the bedside detection can be achieved, the time consumption is about 3-10 min, the sensitivity is high, and the sample consumption is low.

The invention relates to a method for simultaneously detecting 48 stimulants in animal-derived food, which comprises the following steps of: performing extracting and dewatering according to different types of samples, blow-drying the obtained supernate at a proper temperature, performing vortex oscillation and filtration on the residues obtained by dissolving the reagent to form respective to-be-detected solutions, determining the stimulant in the to-be-detected solution and the mixed standard working solution by using a high performance liquid chromatography tandem mass spectrometer, comparing the obtained spectrogram information, and judging that a corresponding target object exists in the sample; according to the peak areas of the stimulant and the corresponding internal standard substance, combining the extraction volume, the sample amount and the dilution ratio of each to-be-detected solution in the acquisition process, and determining the contents of zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an external standard method for quantification, and determining the content of all stimulants except for zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an internal standard method for quantification.

A method of enhancing the performance of chicken comprising administering zilpaterol to the chicken wherein the concentration of zilpaterol is from about 1 ppm to about 13 ppm and is administered every day for a period of about 7 to about 21 days.

The present invention concerns a premix composition for feeding cattle, comprising:

• • a) a premix carrier having an overall particle size comprised between 300 and 400 μm,
  • b) zilpaterol, and
  • c) a surface agent.

The present invention is also related to a method of increasing the rate of weight gain in cattle, comprising the administration to the cattle of feed additives consisting of a premix composition such as described, over a period of at least two weeks.