Method for simultaneously detecting 48 stimulants in feed

Abstract

The invention relates to a method for simultaneously detecting 48 stimulants in a feed, which comprises the following steps: extracting a sample twice by using methanol, blow-drying the merged supernatant, dissolving the obtained residue by using an acetonitrile aqueous solution, carrying out vortex oscillation and filtering to obtain a to-be-detected solution, determining the stimulant in the to-be-detected solution and the mixed standard working solution by using a high performance liquid chromatography tandem mass spectrometer, comparing the obtained spectrogram information, and judging that a corresponding target object exists in the sample; according to the peak areas of the stimulant to be detected and the corresponding internal standard substance, combining the extraction volume, the sample amount and the dilution ratio of each liquid to be detected in the acquisition process, and determining the contents of zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an external standard method for quantification; determining the content of all stimulants except for zilpaterol, fenoterol, higenamine and nandrolone phenylpropionate by adopting an internal standard method for quantification.

Description

technical field [0001] The invention relates to the technical field of stimulants detection, in particular to a method for simultaneously detecting forty-eight stimulants in feed. Background technique [0002] Doping detection is the most important monitoring link in the sports meeting, because doping will be added to the feed, and after the animals eat the doping-containing feed, it will cause different degrees of residues in the muscles and tissues, and then through the biological chain in the Residues in the human body will lead to different degrees of poisoning in the eaters. In order to maintain the fairness and purity of competitive sports and ensure the health and safety of athletes, the detection of doping agents in feed and feed additives is an effective means to control food safety from the source. [0003] However, there are many kinds of stimulants with large structural differences. At present, there are mainly 48 common stimulants that need to be tested, such a...

AI Technical Summary

Problems solved by technology

[0003] However, there are many types of stimulants with large structural differences. At present, there are mainly 48 common stimulants that need to be tested

[0004] The detection technology of doping itself is also constantly developing. Previously, high-performance liquid chromatography (HPLC), gas chromatography (GC), gas chromatography-mass spectrometry (GC / MSD) and other methods were used for detection, but the above 48 Some of the common stimulants need to be derivatized in the detection process, which will cause problems such as complex operation, long detection cycle, and poor sensitivity, which makes it impossible to simultaneously detect forty-eight common stimulants in feed

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